TY  - JOUR
AU  - Milojkov, Dušan V.
AU  - Radosavljević-Mihajlović, Ana S.
AU  - Stanić, Vojislav Đ.
AU  - Nastasijević, Branislav J.
AU  - Radotić, Ksenija
AU  - Janković-Častvan, Ivona
AU  - Živković-Radovanović, Vukosava
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6057
AB  - Nanomaterials based on metal–doped fluorapatite (FAP) have attracted considerable interest as potential next–generation antimicrobial agents. In this study, Cu2+–doped FAP nanocrystals have been successfully synthesized by a neutralization method at room temperature. Their structural, optical, antimicrobial, and hemcompatible properties have been investigated. XRD, FTIR, FESEM, and N2 adsorption–desorption studies indicate the formation of single–phase FAP mesoporous nanopowders, composed of rod–like particles. TEM images confirmed the formation of nanorodes with a length of 60 nm and a width of about 18 nm. Rietveld analysis shows that the Cu2+ ions preferentially substitute Ca2 (6 h) sites in the hexagonal fluorapatite crystal structure. Fluorescence spectroscopy accompanied by MCR–ALS method confirms substitution of Cu2+ ions in FAP crystal lattice with extracting additional d–d band transition at green color from FAP broadband self–activated luminescence in violet–blue color. Antimicrobial studies conducted on Staphylococcus aureus, Escherichia coli and Micrococcus lysodeikticus showed that FAP nanopowder with the highest Cu2+ content have strong bacteriostatic action on Staphylococcus aureus bacterial strain in mediums containing nutrition matters. In addition, this sample in comparison to pure FAP achieved a high percentage of relative reduction of bacterial population for all three species, being >90% in most cases. Fungistatic action is noticed too, throwgh the slowing down mycelium growth of fungus Aspergillus niger, Aspergillus flavus and Penicillium roqueforti and reduction of sporulation of Aspergillus niger species. Cu2+–doped FAP nanocrystals shows a synergistic antimicrobial effect with Cu2+ and F− ions. Concerning the potential biomedical applications, the hemolysis ratios of the Cu2+–doped FAP samples were below 5%. The obtained results pointed out the possible use of the synthesized nanocrystals as broad–spectrum antimicrobial agents for various biomedical and health care preparations.
T2  - Journal of Photochemistry and Photobiology B: Biology
T1  - Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents
VL  - 239
SP  - 112649
DO  - 10.1016/j.jphotobiol.2023.112649
ER  - 

@article{
author = "Milojkov, Dušan V. and Radosavljević-Mihajlović, Ana S. and Stanić, Vojislav Đ. and Nastasijević, Branislav J. and Radotić, Ksenija and Janković-Častvan, Ivona and Živković-Radovanović, Vukosava",
year = "2023",
abstract = "Nanomaterials based on metal–doped fluorapatite (FAP) have attracted considerable interest as potential next–generation antimicrobial agents. In this study, Cu2+–doped FAP nanocrystals have been successfully synthesized by a neutralization method at room temperature. Their structural, optical, antimicrobial, and hemcompatible properties have been investigated. XRD, FTIR, FESEM, and N2 adsorption–desorption studies indicate the formation of single–phase FAP mesoporous nanopowders, composed of rod–like particles. TEM images confirmed the formation of nanorodes with a length of 60 nm and a width of about 18 nm. Rietveld analysis shows that the Cu2+ ions preferentially substitute Ca2 (6 h) sites in the hexagonal fluorapatite crystal structure. Fluorescence spectroscopy accompanied by MCR–ALS method confirms substitution of Cu2+ ions in FAP crystal lattice with extracting additional d–d band transition at green color from FAP broadband self–activated luminescence in violet–blue color. Antimicrobial studies conducted on Staphylococcus aureus, Escherichia coli and Micrococcus lysodeikticus showed that FAP nanopowder with the highest Cu2+ content have strong bacteriostatic action on Staphylococcus aureus bacterial strain in mediums containing nutrition matters. In addition, this sample in comparison to pure FAP achieved a high percentage of relative reduction of bacterial population for all three species, being >90% in most cases. Fungistatic action is noticed too, throwgh the slowing down mycelium growth of fungus Aspergillus niger, Aspergillus flavus and Penicillium roqueforti and reduction of sporulation of Aspergillus niger species. Cu2+–doped FAP nanocrystals shows a synergistic antimicrobial effect with Cu2+ and F− ions. Concerning the potential biomedical applications, the hemolysis ratios of the Cu2+–doped FAP samples were below 5%. The obtained results pointed out the possible use of the synthesized nanocrystals as broad–spectrum antimicrobial agents for various biomedical and health care preparations.",
journal = "Journal of Photochemistry and Photobiology B: Biology",
title = "Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents",
volume = "239",
pages = "112649",
doi = "10.1016/j.jphotobiol.2023.112649"
}

Milojkov, D. V., Radosavljević-Mihajlović, A. S., Stanić, V. Đ., Nastasijević, B. J., Radotić, K., Janković-Častvan, I.,& Živković-Radovanović, V.. (2023). Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents. in Journal of Photochemistry and Photobiology B: Biology, 239, 112649.
https://doi.org/10.1016/j.jphotobiol.2023.112649

Milojkov DV, Radosavljević-Mihajlović AS, Stanić VĐ, Nastasijević BJ, Radotić K, Janković-Častvan I, Živković-Radovanović V. Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents. in Journal of Photochemistry and Photobiology B: Biology. 2023;239:112649.
doi:10.1016/j.jphotobiol.2023.112649 .

Milojkov, Dušan V., Radosavljević-Mihajlović, Ana S., Stanić, Vojislav Đ., Nastasijević, Branislav J., Radotić, Ksenija, Janković-Častvan, Ivona, Živković-Radovanović, Vukosava, "Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents" in Journal of Photochemistry and Photobiology B: Biology, 239 (2023):112649,
https://doi.org/10.1016/j.jphotobiol.2023.112649 . .

TY  - JOUR
AU  - Stanić, Vojislav
AU  - Radosavljević-Mihajlović, Ana S.
AU  - Živković-Radovanović, Vukosava
AU  - Nastasijevic, Branislav
AU  - Marinovic-Cincovic, Milena
AU  - Marković, Jelena
AU  - Budimir, Milica D.
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1679
AB  - Silver doped fluorapatite nanopowders were synthesised by neutralization method, which consists of dissolving Ag2O in solution of HF and H3PO4 and addition to suspension of Ca(OH)(2). The powder XRD, SEM and FTIR studies indicated the formation of a fluorapatite nanomaterials with average length of the particles is about 80 nm and a width of about 15 nm. The FUR studies show that carbonate content in samples is very small and carbonte ions substitute both phosphate and hydroxyl groups in the crystal structure of samples, forming AB-type fluorapatite. Antibacterial studies have demonstrated that all Ag+-doped fluorapatite samples exhibit bactericidal effect against pathogens: Staphylococcus aureus, Micrococcus luteus and Kllebsiela pneumoniae. Antibacterial activity increased with the increase of Ag+ in the samples. The atomic force microscopy studies revealed extensive damage to the bacterial cell envelops in the presence of AW-doped fluorapatite particles which may lead to their death. The synthesized Ag+-doped fluorapatite nanomaterials are promising as antibacterial biomaterials in orthopedics and dentistry. (C) 2015 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Applied Surface Science
T1  - Synthesis, structural characterisation and antibacterial activity of Ag+-doped fluorapatite nanomaterials prepared by neutralization method
VL  - 337
SP  - 72
EP  - 80
DO  - 10.1016/j.apsusc.2015.02.065
ER  - 

@article{
author = "Stanić, Vojislav and Radosavljević-Mihajlović, Ana S. and Živković-Radovanović, Vukosava and Nastasijevic, Branislav and Marinovic-Cincovic, Milena and Marković, Jelena and Budimir, Milica D.",
year = "2015",
abstract = "Silver doped fluorapatite nanopowders were synthesised by neutralization method, which consists of dissolving Ag2O in solution of HF and H3PO4 and addition to suspension of Ca(OH)(2). The powder XRD, SEM and FTIR studies indicated the formation of a fluorapatite nanomaterials with average length of the particles is about 80 nm and a width of about 15 nm. The FUR studies show that carbonate content in samples is very small and carbonte ions substitute both phosphate and hydroxyl groups in the crystal structure of samples, forming AB-type fluorapatite. Antibacterial studies have demonstrated that all Ag+-doped fluorapatite samples exhibit bactericidal effect against pathogens: Staphylococcus aureus, Micrococcus luteus and Kllebsiela pneumoniae. Antibacterial activity increased with the increase of Ag+ in the samples. The atomic force microscopy studies revealed extensive damage to the bacterial cell envelops in the presence of AW-doped fluorapatite particles which may lead to their death. The synthesized Ag+-doped fluorapatite nanomaterials are promising as antibacterial biomaterials in orthopedics and dentistry. (C) 2015 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Applied Surface Science",
title = "Synthesis, structural characterisation and antibacterial activity of Ag+-doped fluorapatite nanomaterials prepared by neutralization method",
volume = "337",
pages = "72-80",
doi = "10.1016/j.apsusc.2015.02.065"
}

Stanić, V., Radosavljević-Mihajlović, A. S., Živković-Radovanović, V., Nastasijevic, B., Marinovic-Cincovic, M., Marković, J.,& Budimir, M. D.. (2015). Synthesis, structural characterisation and antibacterial activity of Ag+-doped fluorapatite nanomaterials prepared by neutralization method. in Applied Surface Science
Elsevier Science Bv, Amsterdam., 337, 72-80.
https://doi.org/10.1016/j.apsusc.2015.02.065

Stanić V, Radosavljević-Mihajlović AS, Živković-Radovanović V, Nastasijevic B, Marinovic-Cincovic M, Marković J, Budimir MD. Synthesis, structural characterisation and antibacterial activity of Ag+-doped fluorapatite nanomaterials prepared by neutralization method. in Applied Surface Science. 2015;337:72-80.
doi:10.1016/j.apsusc.2015.02.065 .

Stanić, Vojislav, Radosavljević-Mihajlović, Ana S., Živković-Radovanović, Vukosava, Nastasijevic, Branislav, Marinovic-Cincovic, Milena, Marković, Jelena, Budimir, Milica D., "Synthesis, structural characterisation and antibacterial activity of Ag+-doped fluorapatite nanomaterials prepared by neutralization method" in Applied Surface Science, 337 (2015):72-80,
https://doi.org/10.1016/j.apsusc.2015.02.065 . .