Gavrović-Jankulović, Marija

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Authority KeyName Variants
orcid::0000-0002-8591-4391
  • Gavrović-Jankulović, Marija (164)
Projects
Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance Ispitivanje strukture i funkcije biološki važnih makromolekula u fiziološkim i patološkim stanjima
Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring
Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness Molecular properties and modifications of some respiratory and nutritional allergens
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200288 (Innovation Center of the Faculty of Chemistry) Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200168 (University of Belgrade, Faculty of Chemistry) Bioanalytical Chemistry Research Group at the Research Center Borstel
COST Action FA1402 entitled: Improving Allergy Risk Assessment Strategy for New Food Proteins (ImpARAS) COST action [FA1402 ImpARAS]
German Academic Exchange Service DAAD Research [91530210-50015537] High-Performance Computing Infrastructure for South East Europe's Research Communities
Rational design and synthesis of biologically active and coordination compounds and functional materials, relevant for (bio)nanotechnology Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection
Interactions of natural products, their derivatives and coordination compounds with proteins and nucleic acids Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200177 (Immunology Research Centre 'Branislav Janković' Torlak, Belgrade) Preclinical investigation of bioactive substances
Ministry of Education, Science and Technological Development of the Republic of Serbia (No. 451-03-9/2021-14) Ministry of Sciences of Croatia
University of Rijeka [13.11.1.2.03] 451-03-00605/2012-16/51 (Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije)
FEBS Collaborative and Experimental Scholarship for Central and Eastern Europe Beneficial effects of dietary bioactive peptides and polyphenols on cardiovascular health in humans
Clinical-epidemiological research of diseases of public health importance in Serbia The pathogenetic mechanism in hematological malignancies
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200019 (University of Belgrade, Institute for the Application of Nuclear Energy - INEP) Biological effects, nutritional intake and status of folate and polysaturate fatty acid (PUFA): improvement of nutrition in Serbia

Author's Bibliography

Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profling Microbial Glycans

Dragačević, Luka; Lopandić, Zorana; Gavrović-Jankulović, Marija; Živković, Irena; Blagojević, Veljko; Polović, Natalija; Minić, Rajna

(Springer, 2022)

TY  - JOUR
AU  - Dragačević, Luka
AU  - Lopandić, Zorana
AU  - Gavrović-Jankulović, Marija
AU  - Živković, Irena
AU  - Blagojević, Veljko
AU  - Polović, Natalija
AU  - Minić, Rajna
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4887
AB  - The surface of microorganisms is covered with carbohydrates, which makes them unique, self-sustaining glycan probes. Lectins are able to bind to these probes, and this interaction can be exploited for selecting microorganisms or novel lectins. To examine lectin-microorganism interactions, we have previously developed an enzyme-linked lectin sorbent assay (ELLSA) with whole bacterial cells. To further test the validity of this methodology, here we compare it with flow cytometry. For this purpose, we used biotinylated recombinantly produced lectin from Musa acuminata (BanLec), this lectin’s recombinantly produced chimera with green fluorescent protein (BanLec-eGFP) and a lectin from Ricinus communis (RCA120), both biotinylated and FITC labeled. Parallel testing showed equivalent results for the two methods, in terms of the presence or absence of binding, with signal intensity yielding high Pearson correlation coefficient of 0.8 for BanLec and 0.95 for RCA120. The ELLSA method demonstrated multiple advantages, such as reliability and convenience for high-throughput analysis; it also required less lectin and yielded more consistent results. As such, ELLSA proved to be a useful tool for profiling microbial glycan structures or testing novel lectins.
PB  - Springer
T2  - Applied Biochemistry and Biotechnology
T2  - Applied Biochemistry and BiotechnologyAppl Biochem Biotechnol
T1  - Comparison of Enzyme-Linked Lectin Sorbent Assay
and Flow Cytometry for Profling Microbial Glycans
VL  - n/a
DO  - 10.1007/s12010-021-03772-w
ER  - 
@article{
author = "Dragačević, Luka and Lopandić, Zorana and Gavrović-Jankulović, Marija and Živković, Irena and Blagojević, Veljko and Polović, Natalija and Minić, Rajna",
year = "2022",
abstract = "The surface of microorganisms is covered with carbohydrates, which makes them unique, self-sustaining glycan probes. Lectins are able to bind to these probes, and this interaction can be exploited for selecting microorganisms or novel lectins. To examine lectin-microorganism interactions, we have previously developed an enzyme-linked lectin sorbent assay (ELLSA) with whole bacterial cells. To further test the validity of this methodology, here we compare it with flow cytometry. For this purpose, we used biotinylated recombinantly produced lectin from Musa acuminata (BanLec), this lectin’s recombinantly produced chimera with green fluorescent protein (BanLec-eGFP) and a lectin from Ricinus communis (RCA120), both biotinylated and FITC labeled. Parallel testing showed equivalent results for the two methods, in terms of the presence or absence of binding, with signal intensity yielding high Pearson correlation coefficient of 0.8 for BanLec and 0.95 for RCA120. The ELLSA method demonstrated multiple advantages, such as reliability and convenience for high-throughput analysis; it also required less lectin and yielded more consistent results. As such, ELLSA proved to be a useful tool for profiling microbial glycan structures or testing novel lectins.",
publisher = "Springer",
journal = "Applied Biochemistry and Biotechnology, Applied Biochemistry and BiotechnologyAppl Biochem Biotechnol",
title = "Comparison of Enzyme-Linked Lectin Sorbent Assay
and Flow Cytometry for Profling Microbial Glycans",
volume = "n/a",
doi = "10.1007/s12010-021-03772-w"
}
Dragačević, L., Lopandić, Z., Gavrović-Jankulović, M., Živković, I., Blagojević, V., Polović, N.,& Minić, R.. (2022). Comparison of Enzyme-Linked Lectin Sorbent Assay
and Flow Cytometry for Profling Microbial Glycans. in Applied Biochemistry and Biotechnology
Springer., n/a.
https://doi.org/10.1007/s12010-021-03772-w
Dragačević L, Lopandić Z, Gavrović-Jankulović M, Živković I, Blagojević V, Polović N, Minić R. Comparison of Enzyme-Linked Lectin Sorbent Assay
and Flow Cytometry for Profling Microbial Glycans. in Applied Biochemistry and Biotechnology. 2022;n/a.
doi:10.1007/s12010-021-03772-w .
Dragačević, Luka, Lopandić, Zorana, Gavrović-Jankulović, Marija, Živković, Irena, Blagojević, Veljko, Polović, Natalija, Minić, Rajna, "Comparison of Enzyme-Linked Lectin Sorbent Assay
and Flow Cytometry for Profling Microbial Glycans" in Applied Biochemistry and Biotechnology, n/a (2022),
https://doi.org/10.1007/s12010-021-03772-w . .

Supplementary data for the article: Dragačević, L.; Lopandić, Z.; Gavrović-Jankulović, M.; Živković, I.; Blagojević, V.; Polović, N.; Minić, R. Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. Appl Biochem Biotechnol 2022. https://doi.org/10.1007/s12010-021-03772-w.

Dragačević, Luka; Lopandić, Zorana; Gavrović-Jankulović, Marija; Živković, Irena; Blagojević, Veljko; Polović, Natalija; Minić, Rajna

(Springer, 2022)

TY  - DATA
AU  - Dragačević, Luka
AU  - Lopandić, Zorana
AU  - Gavrović-Jankulović, Marija
AU  - Živković, Irena
AU  - Blagojević, Veljko
AU  - Polović, Natalija
AU  - Minić, Rajna
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4888
PB  - Springer
T2  - Applied Biochemistry and Biotechnology
T1  - Supplementary data for the article: Dragačević, L.; Lopandić, Z.; Gavrović-Jankulović, M.; Živković, I.; Blagojević, V.; Polović, N.; Minić, R. Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. Appl Biochem Biotechnol 2022. https://doi.org/10.1007/s12010-021-03772-w.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4888
ER  - 
@misc{
author = "Dragačević, Luka and Lopandić, Zorana and Gavrović-Jankulović, Marija and Živković, Irena and Blagojević, Veljko and Polović, Natalija and Minić, Rajna",
year = "2022",
publisher = "Springer",
journal = "Applied Biochemistry and Biotechnology",
title = "Supplementary data for the article: Dragačević, L.; Lopandić, Z.; Gavrović-Jankulović, M.; Živković, I.; Blagojević, V.; Polović, N.; Minić, R. Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. Appl Biochem Biotechnol 2022. https://doi.org/10.1007/s12010-021-03772-w.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4888"
}
Dragačević, L., Lopandić, Z., Gavrović-Jankulović, M., Živković, I., Blagojević, V., Polović, N.,& Minić, R.. (2022). Supplementary data for the article: Dragačević, L.; Lopandić, Z.; Gavrović-Jankulović, M.; Živković, I.; Blagojević, V.; Polović, N.; Minić, R. Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. Appl Biochem Biotechnol 2022. https://doi.org/10.1007/s12010-021-03772-w.. in Applied Biochemistry and Biotechnology
Springer..
https://hdl.handle.net/21.15107/rcub_cherry_4888
Dragačević L, Lopandić Z, Gavrović-Jankulović M, Živković I, Blagojević V, Polović N, Minić R. Supplementary data for the article: Dragačević, L.; Lopandić, Z.; Gavrović-Jankulović, M.; Živković, I.; Blagojević, V.; Polović, N.; Minić, R. Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. Appl Biochem Biotechnol 2022. https://doi.org/10.1007/s12010-021-03772-w.. in Applied Biochemistry and Biotechnology. 2022;.
https://hdl.handle.net/21.15107/rcub_cherry_4888 .
Dragačević, Luka, Lopandić, Zorana, Gavrović-Jankulović, Marija, Živković, Irena, Blagojević, Veljko, Polović, Natalija, Minić, Rajna, "Supplementary data for the article: Dragačević, L.; Lopandić, Z.; Gavrović-Jankulović, M.; Živković, I.; Blagojević, V.; Polović, N.; Minić, R. Comparison of Enzyme-Linked Lectin Sorbent Assay and Flow Cytometry for Profiling Microbial Glycans. Appl Biochem Biotechnol 2022. https://doi.org/10.1007/s12010-021-03772-w." in Applied Biochemistry and Biotechnology (2022),
https://hdl.handle.net/21.15107/rcub_cherry_4888 .

Screening of potential inhibitors of recombinant S-adenosyl-L-homocysteine hydrolase from banana

Đurašinović, Tatjana; Bazović, Vedrana; Trbojević-Ivić, Jovana; Nešić, Andrijana N.; Gavrović-Jankulović, Marija

(Belgrade : Faculty of Chemistry, 2021)

TY  - CONF
AU  - Đurašinović, Tatjana
AU  - Bazović, Vedrana
AU  - Trbojević-Ivić, Jovana
AU  - Nešić, Andrijana N.
AU  - Gavrović-Jankulović, Marija
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5165
PB  - Belgrade : Faculty of Chemistry
C3  - Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia
T1  - Screening of potential inhibitors of recombinant S-adenosyl-L-homocysteine hydrolase from banana
SP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5165
ER  - 
@conference{
author = "Đurašinović, Tatjana and Bazović, Vedrana and Trbojević-Ivić, Jovana and Nešić, Andrijana N. and Gavrović-Jankulović, Marija",
year = "2021",
publisher = "Belgrade : Faculty of Chemistry",
journal = "Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia",
title = "Screening of potential inhibitors of recombinant S-adenosyl-L-homocysteine hydrolase from banana",
pages = "50",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5165"
}
Đurašinović, T., Bazović, V., Trbojević-Ivić, J., Nešić, A. N.,& Gavrović-Jankulović, M.. (2021). Screening of potential inhibitors of recombinant S-adenosyl-L-homocysteine hydrolase from banana. in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia
Belgrade : Faculty of Chemistry., 50.
https://hdl.handle.net/21.15107/rcub_cherry_5165
Đurašinović T, Bazović V, Trbojević-Ivić J, Nešić AN, Gavrović-Jankulović M. Screening of potential inhibitors of recombinant S-adenosyl-L-homocysteine hydrolase from banana. in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia. 2021;:50.
https://hdl.handle.net/21.15107/rcub_cherry_5165 .
Đurašinović, Tatjana, Bazović, Vedrana, Trbojević-Ivić, Jovana, Nešić, Andrijana N., Gavrović-Jankulović, Marija, "Screening of potential inhibitors of recombinant S-adenosyl-L-homocysteine hydrolase from banana" in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia (2021):50,
https://hdl.handle.net/21.15107/rcub_cherry_5165 .

Expression, purification and characterization of recombinant L-phenylalanine dehydrogenase

Pantović, Jelena; Trbojević-Ivić, Jovana; Nešić, Andrijana N.; Gavrović-Jankulović, Marija

(Belgrade : Faculty of Chemistry, 2021)

TY  - CONF
AU  - Pantović, Jelena
AU  - Trbojević-Ivić, Jovana
AU  - Nešić, Andrijana N.
AU  - Gavrović-Jankulović, Marija
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5166
PB  - Belgrade : Faculty of Chemistry
C3  - Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia
T1  - Expression, purification and characterization of recombinant L-phenylalanine dehydrogenase
SP  - 119
EP  - 120
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5166
ER  - 
@conference{
author = "Pantović, Jelena and Trbojević-Ivić, Jovana and Nešić, Andrijana N. and Gavrović-Jankulović, Marija",
year = "2021",
publisher = "Belgrade : Faculty of Chemistry",
journal = "Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia",
title = "Expression, purification and characterization of recombinant L-phenylalanine dehydrogenase",
pages = "119-120",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5166"
}
Pantović, J., Trbojević-Ivić, J., Nešić, A. N.,& Gavrović-Jankulović, M.. (2021). Expression, purification and characterization of recombinant L-phenylalanine dehydrogenase. in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia
Belgrade : Faculty of Chemistry., 119-120.
https://hdl.handle.net/21.15107/rcub_cherry_5166
Pantović J, Trbojević-Ivić J, Nešić AN, Gavrović-Jankulović M. Expression, purification and characterization of recombinant L-phenylalanine dehydrogenase. in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia. 2021;:119-120.
https://hdl.handle.net/21.15107/rcub_cherry_5166 .
Pantović, Jelena, Trbojević-Ivić, Jovana, Nešić, Andrijana N., Gavrović-Jankulović, Marija, "Expression, purification and characterization of recombinant L-phenylalanine dehydrogenase" in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia (2021):119-120,
https://hdl.handle.net/21.15107/rcub_cherry_5166 .

Small molecules attenuate activation of the NF-kB signaling in epithelial cells by Act d 1 kiwifruit allergen

Zlatanova, Milena; Nešić, Andrijana N.; Trbojević-Ivić, Jovana; Gavrović-Jankulović, Marija

(Belgrade : Faculty of Chemistry, 2021)

TY  - CONF
AU  - Zlatanova, Milena
AU  - Nešić, Andrijana N.
AU  - Trbojević-Ivić, Jovana
AU  - Gavrović-Jankulović, Marija
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5167
PB  - Belgrade : Faculty of Chemistry
C3  - Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia
T1  - Small molecules attenuate activation of the NF-kB signaling in epithelial cells by Act d 1 kiwifruit allergen
SP  - 186
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5167
ER  - 
@conference{
author = "Zlatanova, Milena and Nešić, Andrijana N. and Trbojević-Ivić, Jovana and Gavrović-Jankulović, Marija",
year = "2021",
publisher = "Belgrade : Faculty of Chemistry",
journal = "Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia",
title = "Small molecules attenuate activation of the NF-kB signaling in epithelial cells by Act d 1 kiwifruit allergen",
pages = "186",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5167"
}
Zlatanova, M., Nešić, A. N., Trbojević-Ivić, J.,& Gavrović-Jankulović, M.. (2021). Small molecules attenuate activation of the NF-kB signaling in epithelial cells by Act d 1 kiwifruit allergen. in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia
Belgrade : Faculty of Chemistry., 186.
https://hdl.handle.net/21.15107/rcub_cherry_5167
Zlatanova M, Nešić AN, Trbojević-Ivić J, Gavrović-Jankulović M. Small molecules attenuate activation of the NF-kB signaling in epithelial cells by Act d 1 kiwifruit allergen. in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia. 2021;:186.
https://hdl.handle.net/21.15107/rcub_cherry_5167 .
Zlatanova, Milena, Nešić, Andrijana N., Trbojević-Ivić, Jovana, Gavrović-Jankulović, Marija, "Small molecules attenuate activation of the NF-kB signaling in epithelial cells by Act d 1 kiwifruit allergen" in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia (2021):186,
https://hdl.handle.net/21.15107/rcub_cherry_5167 .

BanLec-eGFP Chimera as a Tool for Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms

Lopandić, Zorana; Dragačević, Luka; Popović, Dragan M.; Anđelković, Uroš; Minić, Rajna; Gavrović-Jankulović, Marija

(MDPI, 2021)

TY  - JOUR
AU  - Lopandić, Zorana
AU  - Dragačević, Luka
AU  - Popović, Dragan M.
AU  - Anđelković, Uroš
AU  - Minić, Rajna
AU  - Gavrović-Jankulović, Marija
PY  - 2021
UR  - https://www.mdpi.com/2218-273X/11/2/180
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4489
AB  - Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi. For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli. BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm). More than 65% of the primary structure was confirmed by mass spectrometry. Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant. BanLec-eGFP exhibited binding to mannose residues on different strains of Salmonella in flow cytometry, with especially pronounced binding to a Salmonella Typhi clinical isolate. BanLec-eGFP can be a useful tool for screening high-mannose glycosylation sites on different microorganisms.
PB  - MDPI
T2  - Biomolecules
T2  - Biomolecules
T1  - BanLec-eGFP Chimera as a Tool for Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms
VL  - 11
IS  - 2
SP  - 180
DO  - 10.3390/biom11020180
ER  - 
@article{
author = "Lopandić, Zorana and Dragačević, Luka and Popović, Dragan M. and Anđelković, Uroš and Minić, Rajna and Gavrović-Jankulović, Marija",
year = "2021",
abstract = "Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi. For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli. BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm). More than 65% of the primary structure was confirmed by mass spectrometry. Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant. BanLec-eGFP exhibited binding to mannose residues on different strains of Salmonella in flow cytometry, with especially pronounced binding to a Salmonella Typhi clinical isolate. BanLec-eGFP can be a useful tool for screening high-mannose glycosylation sites on different microorganisms.",
publisher = "MDPI",
journal = "Biomolecules, Biomolecules",
title = "BanLec-eGFP Chimera as a Tool for Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms",
volume = "11",
number = "2",
pages = "180",
doi = "10.3390/biom11020180"
}
Lopandić, Z., Dragačević, L., Popović, D. M., Anđelković, U., Minić, R.,& Gavrović-Jankulović, M.. (2021). BanLec-eGFP Chimera as a Tool for Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms. in Biomolecules
MDPI., 11(2), 180.
https://doi.org/10.3390/biom11020180
Lopandić Z, Dragačević L, Popović DM, Anđelković U, Minić R, Gavrović-Jankulović M. BanLec-eGFP Chimera as a Tool for Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms. in Biomolecules. 2021;11(2):180.
doi:10.3390/biom11020180 .
Lopandić, Zorana, Dragačević, Luka, Popović, Dragan M., Anđelković, Uroš, Minić, Rajna, Gavrović-Jankulović, Marija, "BanLec-eGFP Chimera as a Tool for Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms" in Biomolecules, 11, no. 2 (2021):180,
https://doi.org/10.3390/biom11020180 . .
4
2
2

A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing

Nešić, Andrijana N.; Čavić, Milena; Popović, Milica M.; Gavrović-Jankulović, Marija

(Springer Nature, 2021)

TY  - JOUR
AU  - Nešić, Andrijana N.
AU  - Čavić, Milena
AU  - Popović, Milica M.
AU  - Gavrović-Jankulović, Marija
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4549
AB  - Actinidin (Act d 1), a highly abundant cysteine protease from kiwifruit, is one of the major contributors to the development of kiwifruit allergy. Many studies have focused on the optimization of Act d 1 purification and its role in the development of food allergies. Testing on cell culture monolayers is a common step in the elucidation of food allergen sensitization. In the case of cysteine proteases, an additional activation step with l-cysteine is required before the testing. Hence, we aimed to evaluate whether l-cysteine already present in commonly used cell culture media would suffice for Act d 1 activation. Successfully activated Act d 1 (98.1% of proteolytic activity, as compared to l-cysteine activated Act d 1) was further tested in two commonly used 2D model systems (Caco-2 and HEK293 cells) to evaluate its role on the mRNA expression of cytokines involved in the innate immunity (IL-1β, IL-6, TNFα, TSLP). Furthermore, the contribution of Act d 1 in the promotion of inflammation through regulation of inducible nitric oxide synthase (iNOS) mRNA expression was also examined. These results demonstrate that activation of cysteine proteases can be achieved without previous enzyme incubation in l-cysteine -containing solution. Act d 1 incubated in cell culture medium was able to modulate gene expression of pro-inflammatory cytokines when tested on two model systems of the epithelial barrier. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.
PB  - Springer Nature
T2  - Molecular Biology Reports
T1  - A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing
VL  - 48
IS  - 5
SP  - 4065
EP  - 4072
DO  - 10.1007/s11033-021-06416-8
ER  - 
@article{
author = "Nešić, Andrijana N. and Čavić, Milena and Popović, Milica M. and Gavrović-Jankulović, Marija",
year = "2021",
abstract = "Actinidin (Act d 1), a highly abundant cysteine protease from kiwifruit, is one of the major contributors to the development of kiwifruit allergy. Many studies have focused on the optimization of Act d 1 purification and its role in the development of food allergies. Testing on cell culture monolayers is a common step in the elucidation of food allergen sensitization. In the case of cysteine proteases, an additional activation step with l-cysteine is required before the testing. Hence, we aimed to evaluate whether l-cysteine already present in commonly used cell culture media would suffice for Act d 1 activation. Successfully activated Act d 1 (98.1% of proteolytic activity, as compared to l-cysteine activated Act d 1) was further tested in two commonly used 2D model systems (Caco-2 and HEK293 cells) to evaluate its role on the mRNA expression of cytokines involved in the innate immunity (IL-1β, IL-6, TNFα, TSLP). Furthermore, the contribution of Act d 1 in the promotion of inflammation through regulation of inducible nitric oxide synthase (iNOS) mRNA expression was also examined. These results demonstrate that activation of cysteine proteases can be achieved without previous enzyme incubation in l-cysteine -containing solution. Act d 1 incubated in cell culture medium was able to modulate gene expression of pro-inflammatory cytokines when tested on two model systems of the epithelial barrier. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.",
publisher = "Springer Nature",
journal = "Molecular Biology Reports",
title = "A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing",
volume = "48",
number = "5",
pages = "4065-4072",
doi = "10.1007/s11033-021-06416-8"
}
Nešić, A. N., Čavić, M., Popović, M. M.,& Gavrović-Jankulović, M.. (2021). A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing. in Molecular Biology Reports
Springer Nature., 48(5), 4065-4072.
https://doi.org/10.1007/s11033-021-06416-8
Nešić AN, Čavić M, Popović MM, Gavrović-Jankulović M. A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing. in Molecular Biology Reports. 2021;48(5):4065-4072.
doi:10.1007/s11033-021-06416-8 .
Nešić, Andrijana N., Čavić, Milena, Popović, Milica M., Gavrović-Jankulović, Marija, "A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing" in Molecular Biology Reports, 48, no. 5 (2021):4065-4072,
https://doi.org/10.1007/s11033-021-06416-8 . .

Recombinant Bet v 1-BanLec chimera modulates functional characteristics of peritoneal murine macrophages by promoting IL-10 secretion

Protić-Rosić, Isidora; Nešić, Andrijana N.; Lukić, Ivana; Miljković, Radmila; Popović, Dragan M.; Atanasković-Marković, Marina; Stojanović, Marijana M.; Gavrović-Jankulović, Marija

(Elsevier, 2021)

TY  - JOUR
AU  - Protić-Rosić, Isidora
AU  - Nešić, Andrijana N.
AU  - Lukić, Ivana
AU  - Miljković, Radmila
AU  - Popović, Dragan M.
AU  - Atanasković-Marković, Marina
AU  - Stojanović, Marijana M.
AU  - Gavrović-Jankulović, Marija
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0161589021001905
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4604
AB  - Allergen-specific immunotherapy (AIT) is a desensitizing treatment for allergic diseases that corrects the underlined pathological immune response to innocuous protein antigens, called allergens. Recombinant allergens employed in the AIT allowed the production of well-defined formulations that possessed consistent quality but were often less efficient than natural allergen extracts. Combining recombinant allergens with an adjuvant or immunomodulatory agent could improve AIT efficacy. This study aimed to perform structural and functional characterization of newly designed recombinant chimera composed of the Bet v 1, the major birch pollen allergen, and Banana Lectin (BanLec), TLR2, and CD14 binding protein, for the application in AIT. rBet v 1-BanLec chimera was designed in silico and expressed as a soluble fraction in Escherichia coli. Purified rBet v 1-BanLec (33.4 kDa) retained BanLec-associated biological activity of carbohydrate-binding and preserved IgE reactive epitopes of Bet v 1. The chimera revealed secondary structures with predominant β sheets. The immunomodulatory capacity of rBet v 1-BanLec tested on macrophages showed changes in myeloperoxidase activity, reduced NO production, and significant alterations in the production of cytokines when compared to both rBanLec and rBet v 1. Comparing to rBet v 1, rBet v 1-BanLec was demonstrated to be more efficient promoter of IL-10 production as well as weaker inducer of NO production and secretion of pro-inflammatory cytokines TNFα, and IL-6. The ability of rBet v 1-BanLec to promote IL-10 in together with the preserved 3D structure of Bet v 1 part implies that the construct might exert a beneficial effect in the allergen-specific immunotherapy.
PB  - Elsevier
T2  - Molecular Immunology
T2  - Molecular ImmunologyMolecular Immunology
T1  - Recombinant Bet v 1-BanLec chimera modulates functional characteristics of peritoneal murine macrophages by promoting IL-10 secretion
VL  - 138
SP  - 58
EP  - 67
DO  - 10.1016/j.molimm.2021.06.015
ER  - 
@article{
author = "Protić-Rosić, Isidora and Nešić, Andrijana N. and Lukić, Ivana and Miljković, Radmila and Popović, Dragan M. and Atanasković-Marković, Marina and Stojanović, Marijana M. and Gavrović-Jankulović, Marija",
year = "2021",
abstract = "Allergen-specific immunotherapy (AIT) is a desensitizing treatment for allergic diseases that corrects the underlined pathological immune response to innocuous protein antigens, called allergens. Recombinant allergens employed in the AIT allowed the production of well-defined formulations that possessed consistent quality but were often less efficient than natural allergen extracts. Combining recombinant allergens with an adjuvant or immunomodulatory agent could improve AIT efficacy. This study aimed to perform structural and functional characterization of newly designed recombinant chimera composed of the Bet v 1, the major birch pollen allergen, and Banana Lectin (BanLec), TLR2, and CD14 binding protein, for the application in AIT. rBet v 1-BanLec chimera was designed in silico and expressed as a soluble fraction in Escherichia coli. Purified rBet v 1-BanLec (33.4 kDa) retained BanLec-associated biological activity of carbohydrate-binding and preserved IgE reactive epitopes of Bet v 1. The chimera revealed secondary structures with predominant β sheets. The immunomodulatory capacity of rBet v 1-BanLec tested on macrophages showed changes in myeloperoxidase activity, reduced NO production, and significant alterations in the production of cytokines when compared to both rBanLec and rBet v 1. Comparing to rBet v 1, rBet v 1-BanLec was demonstrated to be more efficient promoter of IL-10 production as well as weaker inducer of NO production and secretion of pro-inflammatory cytokines TNFα, and IL-6. The ability of rBet v 1-BanLec to promote IL-10 in together with the preserved 3D structure of Bet v 1 part implies that the construct might exert a beneficial effect in the allergen-specific immunotherapy.",
publisher = "Elsevier",
journal = "Molecular Immunology, Molecular ImmunologyMolecular Immunology",
title = "Recombinant Bet v 1-BanLec chimera modulates functional characteristics of peritoneal murine macrophages by promoting IL-10 secretion",
volume = "138",
pages = "58-67",
doi = "10.1016/j.molimm.2021.06.015"
}
Protić-Rosić, I., Nešić, A. N., Lukić, I., Miljković, R., Popović, D. M., Atanasković-Marković, M., Stojanović, M. M.,& Gavrović-Jankulović, M.. (2021). Recombinant Bet v 1-BanLec chimera modulates functional characteristics of peritoneal murine macrophages by promoting IL-10 secretion. in Molecular Immunology
Elsevier., 138, 58-67.
https://doi.org/10.1016/j.molimm.2021.06.015
Protić-Rosić I, Nešić AN, Lukić I, Miljković R, Popović DM, Atanasković-Marković M, Stojanović MM, Gavrović-Jankulović M. Recombinant Bet v 1-BanLec chimera modulates functional characteristics of peritoneal murine macrophages by promoting IL-10 secretion. in Molecular Immunology. 2021;138:58-67.
doi:10.1016/j.molimm.2021.06.015 .
Protić-Rosić, Isidora, Nešić, Andrijana N., Lukić, Ivana, Miljković, Radmila, Popović, Dragan M., Atanasković-Marković, Marina, Stojanović, Marijana M., Gavrović-Jankulović, Marija, "Recombinant Bet v 1-BanLec chimera modulates functional characteristics of peritoneal murine macrophages by promoting IL-10 secretion" in Molecular Immunology, 138 (2021):58-67,
https://doi.org/10.1016/j.molimm.2021.06.015 . .

S-Adenosyl-L-Homocysteine Hydrolase Immobilized on Citric Acid-capped Gallium Oxyhydroxide on SWCNTs Modified Electrode for AdoHcy Impedimetric Sensing

Knežević, Sara; Ognjanović, Miloš; Gavrović-Jankulović, Marija; Đurašinović, Tatjana; Antić, Bratislav; Vranješ-Đurić, Sanja; Stanković, Dalibor

(Wiley, 2021)

TY  - JOUR
AU  - Knežević, Sara
AU  - Ognjanović, Miloš
AU  - Gavrović-Jankulović, Marija
AU  - Đurašinović, Tatjana
AU  - Antić, Bratislav
AU  - Vranješ-Đurić, Sanja
AU  - Stanković, Dalibor
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4720
AB  - S-Adenosyl-L-Homocysteine (AdoHcy) is a crucial pathological factor in homocysteine-associated disorders. We developed an impedimetric approach for single drop S-Adenosyl-L-Homocysteine detection, based on S-Adenosyl-L-Homocysteine hydrolase (SAH), produced in the prokaryotic expression system (E. coli) by recombinant DNA technology, immobilized on citrate acid-coated gallium oxyhydroxide dispersed over single-walled carbon nanotubes (CA/GaO(OH)@SWCNT). The proposed biosensor offers detection of AdoHcy, with a limit of detection (LOD) of 0.17 μM. with operating linear range from 1 to 44 μM. The remarkable stability of gallium oxyhydroxide crystal was further improved by citric acid coating and its optical, electrical and physical anisotropy was skipped using SWCNTs.
PB  - Wiley
T2  - Electroanalysis
T1  - S-Adenosyl-L-Homocysteine Hydrolase Immobilized on Citric Acid-capped Gallium Oxyhydroxide on SWCNTs Modified Electrode for AdoHcy Impedimetric Sensing
VL  - n/a
DO  - 10.1002/elan.202100362
ER  - 
@article{
author = "Knežević, Sara and Ognjanović, Miloš and Gavrović-Jankulović, Marija and Đurašinović, Tatjana and Antić, Bratislav and Vranješ-Đurić, Sanja and Stanković, Dalibor",
year = "2021",
abstract = "S-Adenosyl-L-Homocysteine (AdoHcy) is a crucial pathological factor in homocysteine-associated disorders. We developed an impedimetric approach for single drop S-Adenosyl-L-Homocysteine detection, based on S-Adenosyl-L-Homocysteine hydrolase (SAH), produced in the prokaryotic expression system (E. coli) by recombinant DNA technology, immobilized on citrate acid-coated gallium oxyhydroxide dispersed over single-walled carbon nanotubes (CA/GaO(OH)@SWCNT). The proposed biosensor offers detection of AdoHcy, with a limit of detection (LOD) of 0.17 μM. with operating linear range from 1 to 44 μM. The remarkable stability of gallium oxyhydroxide crystal was further improved by citric acid coating and its optical, electrical and physical anisotropy was skipped using SWCNTs.",
publisher = "Wiley",
journal = "Electroanalysis",
title = "S-Adenosyl-L-Homocysteine Hydrolase Immobilized on Citric Acid-capped Gallium Oxyhydroxide on SWCNTs Modified Electrode for AdoHcy Impedimetric Sensing",
volume = "n/a",
doi = "10.1002/elan.202100362"
}
Knežević, S., Ognjanović, M., Gavrović-Jankulović, M., Đurašinović, T., Antić, B., Vranješ-Đurić, S.,& Stanković, D.. (2021). S-Adenosyl-L-Homocysteine Hydrolase Immobilized on Citric Acid-capped Gallium Oxyhydroxide on SWCNTs Modified Electrode for AdoHcy Impedimetric Sensing. in Electroanalysis
Wiley., n/a.
https://doi.org/10.1002/elan.202100362
Knežević S, Ognjanović M, Gavrović-Jankulović M, Đurašinović T, Antić B, Vranješ-Đurić S, Stanković D. S-Adenosyl-L-Homocysteine Hydrolase Immobilized on Citric Acid-capped Gallium Oxyhydroxide on SWCNTs Modified Electrode for AdoHcy Impedimetric Sensing. in Electroanalysis. 2021;n/a.
doi:10.1002/elan.202100362 .
Knežević, Sara, Ognjanović, Miloš, Gavrović-Jankulović, Marija, Đurašinović, Tatjana, Antić, Bratislav, Vranješ-Đurić, Sanja, Stanković, Dalibor, "S-Adenosyl-L-Homocysteine Hydrolase Immobilized on Citric Acid-capped Gallium Oxyhydroxide on SWCNTs Modified Electrode for AdoHcy Impedimetric Sensing" in Electroanalysis, n/a (2021),
https://doi.org/10.1002/elan.202100362 . .
1

Alteration of Trace Elements in Multinodular Goiter, Thyroid Adenoma, and Thyroid Cancer

Stojsavljević, Aleksandar; Rovčanin, Branislav; Jagodić, Jovana; Krstić, Đurđa D.; Paunović, Ivan; Gavrović-Jankulović, Marija; Manojlović, Dragan D.

(Springer, 2021)

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Jagodić, Jovana
AU  - Krstić, Đurđa D.
AU  - Paunović, Ivan
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan D.
PY  - 2021
UR  - https://doi.org/10.1007/s12011-020-02542-9
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4780
AB  - Modest progress has been made in understanding the role of trace elements as endocrine disruptors. The aim of this study was to examine whether there is a change in the content of trace elements in thyroid disease, as well as whether the ratio of elements could be considered a blood marker for thyroid disease. In addition, this study examined the influence of biological and clinical/pathological parameters on the elemental profile. Blood samples from patients diagnosed with multinodular goiter (MNG), thyroid adenoma (TA), and thyroid cancer (TC) were examined and compared with control samples using chemometric analysis. The concentrations of essential (Mn, Co, Cu, Zn, Se) and toxic elements (Ni, As, Cd, Pb, U) were determined by ICP-MS. This study showed for the first time that the content of Mn, Co, Ni, Cu, Zn, Se, and Pb in pathological blood samples was significantly lower compared to the control, while opposite results were obtained for As, Cd, and U. Based on the classification model, the most important trace metals for discrimination of MNG and TC from the control group (CG) were Co and Zn, while Co, Zn, and Mn influenced the distinction of CG from TA. Moreover, it was found that Cu/Zn and U/Se ratios had significantly increased values in pathological blood samples leading to the possibility of establishing new circulating screening markers. These findings can represent significant translational information since these diseases are widespread and the diagnostic procedure is still difficult in many cases.
PB  - Springer
T2  - Biological Trace Element Research
T1  - Alteration of Trace Elements in Multinodular Goiter, Thyroid Adenoma, and Thyroid Cancer
VL  - 199
IS  - 11
SP  - 4055
EP  - 4065
DO  - 10.1007/s12011-020-02542-9
ER  - 
@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Jagodić, Jovana and Krstić, Đurđa D. and Paunović, Ivan and Gavrović-Jankulović, Marija and Manojlović, Dragan D.",
year = "2021",
abstract = "Modest progress has been made in understanding the role of trace elements as endocrine disruptors. The aim of this study was to examine whether there is a change in the content of trace elements in thyroid disease, as well as whether the ratio of elements could be considered a blood marker for thyroid disease. In addition, this study examined the influence of biological and clinical/pathological parameters on the elemental profile. Blood samples from patients diagnosed with multinodular goiter (MNG), thyroid adenoma (TA), and thyroid cancer (TC) were examined and compared with control samples using chemometric analysis. The concentrations of essential (Mn, Co, Cu, Zn, Se) and toxic elements (Ni, As, Cd, Pb, U) were determined by ICP-MS. This study showed for the first time that the content of Mn, Co, Ni, Cu, Zn, Se, and Pb in pathological blood samples was significantly lower compared to the control, while opposite results were obtained for As, Cd, and U. Based on the classification model, the most important trace metals for discrimination of MNG and TC from the control group (CG) were Co and Zn, while Co, Zn, and Mn influenced the distinction of CG from TA. Moreover, it was found that Cu/Zn and U/Se ratios had significantly increased values in pathological blood samples leading to the possibility of establishing new circulating screening markers. These findings can represent significant translational information since these diseases are widespread and the diagnostic procedure is still difficult in many cases.",
publisher = "Springer",
journal = "Biological Trace Element Research",
title = "Alteration of Trace Elements in Multinodular Goiter, Thyroid Adenoma, and Thyroid Cancer",
volume = "199",
number = "11",
pages = "4055-4065",
doi = "10.1007/s12011-020-02542-9"
}
Stojsavljević, A., Rovčanin, B., Jagodić, J., Krstić, Đ. D., Paunović, I., Gavrović-Jankulović, M.,& Manojlović, D. D.. (2021). Alteration of Trace Elements in Multinodular Goiter, Thyroid Adenoma, and Thyroid Cancer. in Biological Trace Element Research
Springer., 199(11), 4055-4065.
https://doi.org/10.1007/s12011-020-02542-9
Stojsavljević A, Rovčanin B, Jagodić J, Krstić ĐD, Paunović I, Gavrović-Jankulović M, Manojlović DD. Alteration of Trace Elements in Multinodular Goiter, Thyroid Adenoma, and Thyroid Cancer. in Biological Trace Element Research. 2021;199(11):4055-4065.
doi:10.1007/s12011-020-02542-9 .
Stojsavljević, Aleksandar, Rovčanin, Branislav, Jagodić, Jovana, Krstić, Đurđa D., Paunović, Ivan, Gavrović-Jankulović, Marija, Manojlović, Dragan D., "Alteration of Trace Elements in Multinodular Goiter, Thyroid Adenoma, and Thyroid Cancer" in Biological Trace Element Research, 199, no. 11 (2021):4055-4065,
https://doi.org/10.1007/s12011-020-02542-9 . .
6
2
5

The first insight into the trace element status of human adrenal gland accompanied by elemental alterations in adrenal adenomas

Jagodić, Jovana; Rovčanin, Branislav; Paunović, Ivan; Jovanović, Milan; Gavrović-Jankulović, Marija; Manojlović, Dragan D.; Stojsavljević, Aleksandar

(Elsevier, 2021)

TY  - JOUR
AU  - Jagodić, Jovana
AU  - Rovčanin, Branislav
AU  - Paunović, Ivan
AU  - Jovanović, Milan
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan D.
AU  - Stojsavljević, Aleksandar
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4787
AB  - Background
The baseline status of trace metals in adrenal tissue is unresolved, while the elemental profile for any adrenal pathology has not been examined so far. This study aimed to determine the baseline status of important toxic (Ni, As, Cd, Pb, Th, U) and essential trace elements (Mn, Co, Cu, Zn, Se) in healthy adrenal tissues (HATs) as well as to examine whether there are alterations in the elemental composition of adenomatous adrenal tissues (AATs). Furthermore, this study aimed to find potential trace metals that could play a role in the pathogenesis of adrenal adenoma (AA).

Methods
The study included 45 patients diagnosed with AA. Impacts of relevant parameters such as gender, age, smoking habits and nodular sizes were considered. All samples were subjected to microwave digestion and the trace elements were determined by inductively coupled plasma mass spectrometry (ICP-MS).

Results
This is the first study that provided an insight into the elemental status of HATs. It was also shown that AATs had altered trace metal contents. Compared to HATs, the most significant findings were related to the high content of essential (Cu, Mn, Se, Zn) and Pb as a non-essential metal. Although gender, age and smoking habits had a modest effect on metal profiles, the most significant alterations were related to the nodular diameter above 4 cm, indicating that the growth of benign tumor could influence changes in elemental composition.

Conclusion
For the first time the baseline contents of essential and toxic trace metals in HATs were determined. The results of this study may highlight the role of toxic and essential trace metals in AAs and could provide new insights into the molecular basis of pathophysiological changes caused by the hazardous effects of trace metals on adrenal structure and function.
PB  - Elsevier
T2  - Journal of Trace Elements in Medicine and Biology
T1  - The first insight into the trace element status of human adrenal gland accompanied by elemental alterations in adrenal adenomas
VL  - 63
SP  - 126658
DO  - 10.1016/j.jtemb.2020.126658
ER  - 
@article{
author = "Jagodić, Jovana and Rovčanin, Branislav and Paunović, Ivan and Jovanović, Milan and Gavrović-Jankulović, Marija and Manojlović, Dragan D. and Stojsavljević, Aleksandar",
year = "2021",
abstract = "Background
The baseline status of trace metals in adrenal tissue is unresolved, while the elemental profile for any adrenal pathology has not been examined so far. This study aimed to determine the baseline status of important toxic (Ni, As, Cd, Pb, Th, U) and essential trace elements (Mn, Co, Cu, Zn, Se) in healthy adrenal tissues (HATs) as well as to examine whether there are alterations in the elemental composition of adenomatous adrenal tissues (AATs). Furthermore, this study aimed to find potential trace metals that could play a role in the pathogenesis of adrenal adenoma (AA).

Methods
The study included 45 patients diagnosed with AA. Impacts of relevant parameters such as gender, age, smoking habits and nodular sizes were considered. All samples were subjected to microwave digestion and the trace elements were determined by inductively coupled plasma mass spectrometry (ICP-MS).

Results
This is the first study that provided an insight into the elemental status of HATs. It was also shown that AATs had altered trace metal contents. Compared to HATs, the most significant findings were related to the high content of essential (Cu, Mn, Se, Zn) and Pb as a non-essential metal. Although gender, age and smoking habits had a modest effect on metal profiles, the most significant alterations were related to the nodular diameter above 4 cm, indicating that the growth of benign tumor could influence changes in elemental composition.

Conclusion
For the first time the baseline contents of essential and toxic trace metals in HATs were determined. The results of this study may highlight the role of toxic and essential trace metals in AAs and could provide new insights into the molecular basis of pathophysiological changes caused by the hazardous effects of trace metals on adrenal structure and function.",
publisher = "Elsevier",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "The first insight into the trace element status of human adrenal gland accompanied by elemental alterations in adrenal adenomas",
volume = "63",
pages = "126658",
doi = "10.1016/j.jtemb.2020.126658"
}
Jagodić, J., Rovčanin, B., Paunović, I., Jovanović, M., Gavrović-Jankulović, M., Manojlović, D. D.,& Stojsavljević, A.. (2021). The first insight into the trace element status of human adrenal gland accompanied by elemental alterations in adrenal adenomas. in Journal of Trace Elements in Medicine and Biology
Elsevier., 63, 126658.
https://doi.org/10.1016/j.jtemb.2020.126658
Jagodić J, Rovčanin B, Paunović I, Jovanović M, Gavrović-Jankulović M, Manojlović DD, Stojsavljević A. The first insight into the trace element status of human adrenal gland accompanied by elemental alterations in adrenal adenomas. in Journal of Trace Elements in Medicine and Biology. 2021;63:126658.
doi:10.1016/j.jtemb.2020.126658 .
Jagodić, Jovana, Rovčanin, Branislav, Paunović, Ivan, Jovanović, Milan, Gavrović-Jankulović, Marija, Manojlović, Dragan D., Stojsavljević, Aleksandar, "The first insight into the trace element status of human adrenal gland accompanied by elemental alterations in adrenal adenomas" in Journal of Trace Elements in Medicine and Biology, 63 (2021):126658,
https://doi.org/10.1016/j.jtemb.2020.126658 . .
2
2
4

IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein

Lopandić, Zorana; Protić-Rosić, Isidora; Todorović, Aleksandra; Glamočlija, Sofija; Gnjatović, Marija; Ćujic, Danica; Gavrović-Jankulović, Marija

(MDPI, 2021)

TY  - JOUR
AU  - Lopandić, Zorana
AU  - Protić-Rosić, Isidora
AU  - Todorović, Aleksandra
AU  - Glamočlija, Sofija
AU  - Gnjatović, Marija
AU  - Ćujic, Danica
AU  - Gavrović-Jankulović, Marija
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4800
AB  - Diagnostic evaluation of specific antibodies against the SARS-CoV-2 virus is mainly based on spike (S) and nucleocapsid (N) proteins. Despite the critical functions in virus infection and contribution to the pattern of immunodominance in COVID-19, exploitation of the most abundant membrane (M) protein in the SARS-CoV-2 serology tests is minimal. This study investigated the recombinant M protein’s immunoreactivity with the sera from COVID-19 convalescents. In silico designed protein was created from the outer N-terminal part (19 aa) and internal C-terminal tail (101–222 aa) of the M protein (YP_009724393.1) and was recombinantly produced and purified. The designed M protein (16,498.74 Da, pI 8.79) revealed both IgM and IgG reactivity with serum samples from COVID-19 convalescents in Western blot. In ELISA, more than 93% (28/30) of COVID-19 sera were positive for IgM detection, and more than 96% (29/30) were positive for specific IgG detection to M protein. Based on the capacity to provoke an immune response and its strong antigenic properties, as shown here, and the fact that it is also involved in the virion entry into host cells, the M protein of the SARS-CoV-2 virus as a good antigen has the potential in diagnostic purposes and vaccine design.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein
VL  - 22
IS  - 9
SP  - 4951
DO  - 10.3390/ijms22094951
ER  - 
@article{
author = "Lopandić, Zorana and Protić-Rosić, Isidora and Todorović, Aleksandra and Glamočlija, Sofija and Gnjatović, Marija and Ćujic, Danica and Gavrović-Jankulović, Marija",
year = "2021",
abstract = "Diagnostic evaluation of specific antibodies against the SARS-CoV-2 virus is mainly based on spike (S) and nucleocapsid (N) proteins. Despite the critical functions in virus infection and contribution to the pattern of immunodominance in COVID-19, exploitation of the most abundant membrane (M) protein in the SARS-CoV-2 serology tests is minimal. This study investigated the recombinant M protein’s immunoreactivity with the sera from COVID-19 convalescents. In silico designed protein was created from the outer N-terminal part (19 aa) and internal C-terminal tail (101–222 aa) of the M protein (YP_009724393.1) and was recombinantly produced and purified. The designed M protein (16,498.74 Da, pI 8.79) revealed both IgM and IgG reactivity with serum samples from COVID-19 convalescents in Western blot. In ELISA, more than 93% (28/30) of COVID-19 sera were positive for IgM detection, and more than 96% (29/30) were positive for specific IgG detection to M protein. Based on the capacity to provoke an immune response and its strong antigenic properties, as shown here, and the fact that it is also involved in the virion entry into host cells, the M protein of the SARS-CoV-2 virus as a good antigen has the potential in diagnostic purposes and vaccine design.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein",
volume = "22",
number = "9",
pages = "4951",
doi = "10.3390/ijms22094951"
}
Lopandić, Z., Protić-Rosić, I., Todorović, A., Glamočlija, S., Gnjatović, M., Ćujic, D.,& Gavrović-Jankulović, M.. (2021). IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein. in International Journal of Molecular Sciences
MDPI., 22(9), 4951.
https://doi.org/10.3390/ijms22094951
Lopandić Z, Protić-Rosić I, Todorović A, Glamočlija S, Gnjatović M, Ćujic D, Gavrović-Jankulović M. IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein. in International Journal of Molecular Sciences. 2021;22(9):4951.
doi:10.3390/ijms22094951 .
Lopandić, Zorana, Protić-Rosić, Isidora, Todorović, Aleksandra, Glamočlija, Sofija, Gnjatović, Marija, Ćujic, Danica, Gavrović-Jankulović, Marija, "IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein" in International Journal of Molecular Sciences, 22, no. 9 (2021):4951,
https://doi.org/10.3390/ijms22094951 . .
7
1
2

Impact of bee venom and melittin on apoptosis and biotransformation in colorectal carcinoma cell lines

Nikodijević, Danijela D.; Milutinović, Milena G.; Cvetković, Danijela M.; Ćupurdija, Maja Đ.; Jovanović, Milena M.; Mrkić, Ivan V.; Gavrović-Jankulović, Marija; Marković, Snežana D.

(Taylor & Francis, 2021)

TY  - JOUR
AU  - Nikodijević, Danijela D.
AU  - Milutinović, Milena G.
AU  - Cvetković, Danijela M.
AU  - Ćupurdija, Maja Đ.
AU  - Jovanović, Milena M.
AU  - Mrkić, Ivan V.
AU  - Gavrović-Jankulović, Marija
AU  - Marković, Snežana D.
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4974
AB  - This study provides data about anticancer properties of bee venom and its dominant compound, melittin on colorectal carcinoma cells (HCT-116 and SW-480) in regard to their proapoptotic activity and expression of genes involved in biotransformation process. Based on results, they are strong cytotoxins, where the melittin showed also selectivity against cancer cells compared to normal, HaCat. They induce proapoptotic activity by affecting apoptosis signaling molecules (Fas receptors, caspase 9, and members of Bcl-2 family of proteins) and mainly suppress expression of genes involved in their biotransformation, suggesting their ability to develop the resistance of colorectal cancer cells.
PB  - Taylor & Francis
T2  - Toxin Reviews
T1  - Impact of bee venom and melittin on apoptosis and biotransformation in colorectal carcinoma cell lines
VL  - 40
IS  - 4
SP  - 1272
EP  - 1279
DO  - 10.1080/15569543.2019.1680564
ER  - 
@article{
author = "Nikodijević, Danijela D. and Milutinović, Milena G. and Cvetković, Danijela M. and Ćupurdija, Maja Đ. and Jovanović, Milena M. and Mrkić, Ivan V. and Gavrović-Jankulović, Marija and Marković, Snežana D.",
year = "2021",
abstract = "This study provides data about anticancer properties of bee venom and its dominant compound, melittin on colorectal carcinoma cells (HCT-116 and SW-480) in regard to their proapoptotic activity and expression of genes involved in biotransformation process. Based on results, they are strong cytotoxins, where the melittin showed also selectivity against cancer cells compared to normal, HaCat. They induce proapoptotic activity by affecting apoptosis signaling molecules (Fas receptors, caspase 9, and members of Bcl-2 family of proteins) and mainly suppress expression of genes involved in their biotransformation, suggesting their ability to develop the resistance of colorectal cancer cells.",
publisher = "Taylor & Francis",
journal = "Toxin Reviews",
title = "Impact of bee venom and melittin on apoptosis and biotransformation in colorectal carcinoma cell lines",
volume = "40",
number = "4",
pages = "1272-1279",
doi = "10.1080/15569543.2019.1680564"
}
Nikodijević, D. D., Milutinović, M. G., Cvetković, D. M., Ćupurdija, M. Đ., Jovanović, M. M., Mrkić, I. V., Gavrović-Jankulović, M.,& Marković, S. D.. (2021). Impact of bee venom and melittin on apoptosis and biotransformation in colorectal carcinoma cell lines. in Toxin Reviews
Taylor & Francis., 40(4), 1272-1279.
https://doi.org/10.1080/15569543.2019.1680564
Nikodijević DD, Milutinović MG, Cvetković DM, Ćupurdija MĐ, Jovanović MM, Mrkić IV, Gavrović-Jankulović M, Marković SD. Impact of bee venom and melittin on apoptosis and biotransformation in colorectal carcinoma cell lines. in Toxin Reviews. 2021;40(4):1272-1279.
doi:10.1080/15569543.2019.1680564 .
Nikodijević, Danijela D., Milutinović, Milena G., Cvetković, Danijela M., Ćupurdija, Maja Đ., Jovanović, Milena M., Mrkić, Ivan V., Gavrović-Jankulović, Marija, Marković, Snežana D., "Impact of bee venom and melittin on apoptosis and biotransformation in colorectal carcinoma cell lines" in Toxin Reviews, 40, no. 4 (2021):1272-1279,
https://doi.org/10.1080/15569543.2019.1680564 . .
2
3
2

Supplementary data for the article: Nikodijević, D. D.; Milutinović, M. G.; Cvetković, D. M.; Ćupurdija, M. Đ.; Jovanović, M. M.; Mrkić, I. V.; Jankulović-Gavrović, M. Đ.; Marković, S. D. Impact of Bee Venom and Melittin on Apoptosis and Biotransformation in Colorectal Carcinoma Cell Lines. Toxin Reviews 2021, 40 (4), 1272–1279. https://doi.org/10.1080/15569543.2019.1680564.

Nikodijević, Danijela D.; Milutinović, Milena G.; Cvetković, Danijela M.; Ćupurdija, Maja Đ.; Jovanović, Milena M.; Mrkić, Ivan V.; Gavrović-Jankulović, Marija; Marković, Snežana D.

(Taylor & Francis, 2021)

TY  - DATA
AU  - Nikodijević, Danijela D.
AU  - Milutinović, Milena G.
AU  - Cvetković, Danijela M.
AU  - Ćupurdija, Maja Đ.
AU  - Jovanović, Milena M.
AU  - Mrkić, Ivan V.
AU  - Gavrović-Jankulović, Marija
AU  - Marković, Snežana D.
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4975
PB  - Taylor & Francis
T2  - Toxin Reviews
T1  - Supplementary data for the article: Nikodijević, D. D.; Milutinović, M. G.; Cvetković, D. M.; Ćupurdija, M. Đ.; Jovanović, M. M.; Mrkić, I. V.; Jankulović-Gavrović, M. Đ.; Marković, S. D. Impact of Bee Venom and Melittin on Apoptosis and Biotransformation in Colorectal Carcinoma Cell Lines. Toxin Reviews 2021, 40 (4), 1272–1279. https://doi.org/10.1080/15569543.2019.1680564.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4975
ER  - 
@misc{
author = "Nikodijević, Danijela D. and Milutinović, Milena G. and Cvetković, Danijela M. and Ćupurdija, Maja Đ. and Jovanović, Milena M. and Mrkić, Ivan V. and Gavrović-Jankulović, Marija and Marković, Snežana D.",
year = "2021",
publisher = "Taylor & Francis",
journal = "Toxin Reviews",
title = "Supplementary data for the article: Nikodijević, D. D.; Milutinović, M. G.; Cvetković, D. M.; Ćupurdija, M. Đ.; Jovanović, M. M.; Mrkić, I. V.; Jankulović-Gavrović, M. Đ.; Marković, S. D. Impact of Bee Venom and Melittin on Apoptosis and Biotransformation in Colorectal Carcinoma Cell Lines. Toxin Reviews 2021, 40 (4), 1272–1279. https://doi.org/10.1080/15569543.2019.1680564.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4975"
}
Nikodijević, D. D., Milutinović, M. G., Cvetković, D. M., Ćupurdija, M. Đ., Jovanović, M. M., Mrkić, I. V., Gavrović-Jankulović, M.,& Marković, S. D.. (2021). Supplementary data for the article: Nikodijević, D. D.; Milutinović, M. G.; Cvetković, D. M.; Ćupurdija, M. Đ.; Jovanović, M. M.; Mrkić, I. V.; Jankulović-Gavrović, M. Đ.; Marković, S. D. Impact of Bee Venom and Melittin on Apoptosis and Biotransformation in Colorectal Carcinoma Cell Lines. Toxin Reviews 2021, 40 (4), 1272–1279. https://doi.org/10.1080/15569543.2019.1680564.. in Toxin Reviews
Taylor & Francis..
https://hdl.handle.net/21.15107/rcub_cherry_4975
Nikodijević DD, Milutinović MG, Cvetković DM, Ćupurdija MĐ, Jovanović MM, Mrkić IV, Gavrović-Jankulović M, Marković SD. Supplementary data for the article: Nikodijević, D. D.; Milutinović, M. G.; Cvetković, D. M.; Ćupurdija, M. Đ.; Jovanović, M. M.; Mrkić, I. V.; Jankulović-Gavrović, M. Đ.; Marković, S. D. Impact of Bee Venom and Melittin on Apoptosis and Biotransformation in Colorectal Carcinoma Cell Lines. Toxin Reviews 2021, 40 (4), 1272–1279. https://doi.org/10.1080/15569543.2019.1680564.. in Toxin Reviews. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4975 .
Nikodijević, Danijela D., Milutinović, Milena G., Cvetković, Danijela M., Ćupurdija, Maja Đ., Jovanović, Milena M., Mrkić, Ivan V., Gavrović-Jankulović, Marija, Marković, Snežana D., "Supplementary data for the article: Nikodijević, D. D.; Milutinović, M. G.; Cvetković, D. M.; Ćupurdija, M. Đ.; Jovanović, M. M.; Mrkić, I. V.; Jankulović-Gavrović, M. Đ.; Marković, S. D. Impact of Bee Venom and Melittin on Apoptosis and Biotransformation in Colorectal Carcinoma Cell Lines. Toxin Reviews 2021, 40 (4), 1272–1279. https://doi.org/10.1080/15569543.2019.1680564." in Toxin Reviews (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4975 .

Impact of tree pollen distribution on allergic diseases in serbia: Evidence of implementation of allergen immunotherapy to Betula verrucosa

Minić, Rajna; Josipović, Mirjana; Tomić Spirić, Vesna; Gavrović-Jankulović, Marija; Perić Popadić, Aleksandra; Prokopijević, Ivana; Ljubičić, Ana; Stamenković, Danijela; Burazer, Lidija M.

(MDPI, 2020)

TY  - JOUR
AU  - Minić, Rajna
AU  - Josipović, Mirjana
AU  - Tomić Spirić, Vesna
AU  - Gavrović-Jankulović, Marija
AU  - Perić Popadić, Aleksandra
AU  - Prokopijević, Ivana
AU  - Ljubičić, Ana
AU  - Stamenković, Danijela
AU  - Burazer, Lidija M.
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3883
AB  - Background and objectives: The relationship between air pollen quantity and the sensitization of allergic patients is crucial for both the diagnosis and treatment of allergic diseases. Weather conditions influence the distribution of allergenic pollen and increases in pollen concentration may negatively affect the health of allergic patients. The aim of this study was to analyze the implementation of allergen immunotherapy with regard to air pollen concentration. Material and Methods: Here we examined the relationship between Betula air pollen concentration and the usage of Betula verrucosa allergen immunotherapy in Serbia. Examination covered the period from 2015 to 2018. Measurement of airborne pollen concentration was performed with Lanzoni volumetric pollen traps. The evidence of the usage of sublingual allergen immunotherapy (SLIT) was gathered from patients with documented sensitization to specific pollen. Results: During this period tree pollens were represented with 58% ± 21% of all measured air pollen species, while Betula pollen represented 15% ± 8% of all tree pollens. Betula pollination peaked in April. Allergen immunotherapy to Betula verrucosa in Serbia is entirely conducted as sublingual immunotherapy and represents 47.1% ± 1.4% of issued tree pollen SLIT. The use of pollen SLIT increased by 68% from 2015 to 2018, with an even greater increase in usage recorded for Betula SLIT—80%. Conclusions: This analysis shows a clear causative relationship between pollination and the type/prevalence of applied allergen immunotherapy. Information about the flowering seasons of allergenic plants is very important for people who suffer from allergy, for clinical allergologists, as well as for governing authorities. The presented data is of practical importance to the proper timing of immunotherapy initiation and of importance for urban landscaping. The obtained data can be the starting point for the instatement of a thorough epidemiological study and the inclusion of Serbia on the pollen map of Europe.
PB  - MDPI
T2  - Medicina (Lithuania)
T1  - Impact of tree pollen distribution on allergic diseases in serbia: Evidence of implementation of allergen immunotherapy to Betula verrucosa
VL  - 56
IS  - 2
DO  - 10.3390/medicina56020059
ER  - 
@article{
author = "Minić, Rajna and Josipović, Mirjana and Tomić Spirić, Vesna and Gavrović-Jankulović, Marija and Perić Popadić, Aleksandra and Prokopijević, Ivana and Ljubičić, Ana and Stamenković, Danijela and Burazer, Lidija M.",
year = "2020",
abstract = "Background and objectives: The relationship between air pollen quantity and the sensitization of allergic patients is crucial for both the diagnosis and treatment of allergic diseases. Weather conditions influence the distribution of allergenic pollen and increases in pollen concentration may negatively affect the health of allergic patients. The aim of this study was to analyze the implementation of allergen immunotherapy with regard to air pollen concentration. Material and Methods: Here we examined the relationship between Betula air pollen concentration and the usage of Betula verrucosa allergen immunotherapy in Serbia. Examination covered the period from 2015 to 2018. Measurement of airborne pollen concentration was performed with Lanzoni volumetric pollen traps. The evidence of the usage of sublingual allergen immunotherapy (SLIT) was gathered from patients with documented sensitization to specific pollen. Results: During this period tree pollens were represented with 58% ± 21% of all measured air pollen species, while Betula pollen represented 15% ± 8% of all tree pollens. Betula pollination peaked in April. Allergen immunotherapy to Betula verrucosa in Serbia is entirely conducted as sublingual immunotherapy and represents 47.1% ± 1.4% of issued tree pollen SLIT. The use of pollen SLIT increased by 68% from 2015 to 2018, with an even greater increase in usage recorded for Betula SLIT—80%. Conclusions: This analysis shows a clear causative relationship between pollination and the type/prevalence of applied allergen immunotherapy. Information about the flowering seasons of allergenic plants is very important for people who suffer from allergy, for clinical allergologists, as well as for governing authorities. The presented data is of practical importance to the proper timing of immunotherapy initiation and of importance for urban landscaping. The obtained data can be the starting point for the instatement of a thorough epidemiological study and the inclusion of Serbia on the pollen map of Europe.",
publisher = "MDPI",
journal = "Medicina (Lithuania)",
title = "Impact of tree pollen distribution on allergic diseases in serbia: Evidence of implementation of allergen immunotherapy to Betula verrucosa",
volume = "56",
number = "2",
doi = "10.3390/medicina56020059"
}
Minić, R., Josipović, M., Tomić Spirić, V., Gavrović-Jankulović, M., Perić Popadić, A., Prokopijević, I., Ljubičić, A., Stamenković, D.,& Burazer, L. M.. (2020). Impact of tree pollen distribution on allergic diseases in serbia: Evidence of implementation of allergen immunotherapy to Betula verrucosa. in Medicina (Lithuania)
MDPI., 56(2).
https://doi.org/10.3390/medicina56020059
Minić R, Josipović M, Tomić Spirić V, Gavrović-Jankulović M, Perić Popadić A, Prokopijević I, Ljubičić A, Stamenković D, Burazer LM. Impact of tree pollen distribution on allergic diseases in serbia: Evidence of implementation of allergen immunotherapy to Betula verrucosa. in Medicina (Lithuania). 2020;56(2).
doi:10.3390/medicina56020059 .
Minić, Rajna, Josipović, Mirjana, Tomić Spirić, Vesna, Gavrović-Jankulović, Marija, Perić Popadić, Aleksandra, Prokopijević, Ivana, Ljubičić, Ana, Stamenković, Danijela, Burazer, Lidija M., "Impact of tree pollen distribution on allergic diseases in serbia: Evidence of implementation of allergen immunotherapy to Betula verrucosa" in Medicina (Lithuania), 56, no. 2 (2020),
https://doi.org/10.3390/medicina56020059 . .
1
1
1
1

Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors

Stojsavljević, Aleksandar; Vujotić, Ljiljana; Rovčanin, Branislav; Borković-Mitić, Slavica S.; Gavrović-Jankulović, Marija; Manojlović, Dragan D.

(Nature Research, 2020)

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Vujotić, Ljiljana
AU  - Rovčanin, Branislav
AU  - Borković-Mitić, Slavica S.
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan D.
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3885
AB  - The pathogenesis of malignant brain tumors (MBTs) should be better understood due to the evident association between prolonged exposure to metals and increased risk of MBTs. The present research aimed to find trace metals that could contribute to the pathogenesis of MBTs. Essential trace elements (Mn, Co, Zn, Cu, Se) and relevant toxic metals (Al, Ni, As, Sr, Cd, Ce, Pt, Pb, U) in the serum, cell fraction (CF), cerebrospinal fluid (CSF) and cancerous tissue (CT) samples of MBT patients were analyzed. The results were compared with sex- and age-matched control groups. For the first time, this research showed that elemental profiles of serum, CF, CSF and CT samples in MBT patients were significantly altered compared to the appropriate controls, as well as that higher contents of trace elements (particularly Mn, Se, and Pb) could be involved in the pathogenesis of MBTs. However, the most noticeable change found was the elevated U content, indicating its considerable role as a major cerebral discriminator of the presence/absence of MBTs. The U/Se ratio could be considered as an appropriate blood marker in diagnostic MBT evaluation. The reported results could contribute to better understanding of the poorly understood pathogenesis of MBTs. Furthermore, the reported results could highlight a molecular basis for the pathophysiological changes caused by the hazardous effects of trace metals on brain homeostasis.
PB  - Nature Research
T2  - Scientific Reports
T1  - Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors
VL  - 10
IS  - 1
DO  - 10.1038/s41598-020-60774-0
ER  - 
@article{
author = "Stojsavljević, Aleksandar and Vujotić, Ljiljana and Rovčanin, Branislav and Borković-Mitić, Slavica S. and Gavrović-Jankulović, Marija and Manojlović, Dragan D.",
year = "2020",
abstract = "The pathogenesis of malignant brain tumors (MBTs) should be better understood due to the evident association between prolonged exposure to metals and increased risk of MBTs. The present research aimed to find trace metals that could contribute to the pathogenesis of MBTs. Essential trace elements (Mn, Co, Zn, Cu, Se) and relevant toxic metals (Al, Ni, As, Sr, Cd, Ce, Pt, Pb, U) in the serum, cell fraction (CF), cerebrospinal fluid (CSF) and cancerous tissue (CT) samples of MBT patients were analyzed. The results were compared with sex- and age-matched control groups. For the first time, this research showed that elemental profiles of serum, CF, CSF and CT samples in MBT patients were significantly altered compared to the appropriate controls, as well as that higher contents of trace elements (particularly Mn, Se, and Pb) could be involved in the pathogenesis of MBTs. However, the most noticeable change found was the elevated U content, indicating its considerable role as a major cerebral discriminator of the presence/absence of MBTs. The U/Se ratio could be considered as an appropriate blood marker in diagnostic MBT evaluation. The reported results could contribute to better understanding of the poorly understood pathogenesis of MBTs. Furthermore, the reported results could highlight a molecular basis for the pathophysiological changes caused by the hazardous effects of trace metals on brain homeostasis.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors",
volume = "10",
number = "1",
doi = "10.1038/s41598-020-60774-0"
}
Stojsavljević, A., Vujotić, L., Rovčanin, B., Borković-Mitić, S. S., Gavrović-Jankulović, M.,& Manojlović, D. D.. (2020). Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors. in Scientific Reports
Nature Research., 10(1).
https://doi.org/10.1038/s41598-020-60774-0
Stojsavljević A, Vujotić L, Rovčanin B, Borković-Mitić SS, Gavrović-Jankulović M, Manojlović DD. Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors. in Scientific Reports. 2020;10(1).
doi:10.1038/s41598-020-60774-0 .
Stojsavljević, Aleksandar, Vujotić, Ljiljana, Rovčanin, Branislav, Borković-Mitić, Slavica S., Gavrović-Jankulović, Marija, Manojlović, Dragan D., "Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors" in Scientific Reports, 10, no. 1 (2020),
https://doi.org/10.1038/s41598-020-60774-0 . .
12
11
12

Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease

Stojsavljević, Aleksandar; Rovčanin, Branislav; Krstić, Đurđa D.; Borković-Mitić, Slavica S.; Paunović, Ivan; Diklić, Aleksandar; Gavrović-Jankulović, Marija; Manojlović, Dragan D.

(2020)

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa D.
AU  - Borković-Mitić, Slavica S.
AU  - Paunović, Ivan
AU  - Diklić, Aleksandar
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan D.
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3955
AB  - The background level of lead (Pb) in drinking water in Serbia represents a significant health issue. The aim of study was to examine the content of significant toxic and essential trace metals (Mn, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U) in the thyroid tissues with diagnosed colloid goiter (CG). Different variables were investigated (sex, age, smoking habits, goiter size, and previous history of any thyroid disease). The study was designed to compare the goiter tissues (GTs) to sex- and age-matched healthy- and malignant-thyroid tissues (HTTs and MTTs, respectively). All trace metals were quantified by inductively coupled plasma mass spectrometry (ICP-MS). The reduced content of essential elements (Mn and Se) and increased content of toxic metals (Pb, Th, and U) were found in GTs compared with HTTs. The increased content of the same toxic metals separated GTs from MTTs, as well as reduced content of Mn and Cd. Our results indicated that Pb acts as the main goitrogen, which could highlight its role in the unknown etiology of CG disease. Pb/Se ratio could be considered as a relevant parameter for the tissue separation of GTs from HTTs and/or MTTs. This research also provides novel data on the effects of metal pollutants on thyroid health hazards. Graphical Abstract: [Figure not available: see fulltext.].
T2  - Exposure and Health
T1  - Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease
VL  - 12
IS  - 2
SP  - 255
EP  - 264
DO  - 10.1007/s12403-019-00309-9
ER  - 
@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa D. and Borković-Mitić, Slavica S. and Paunović, Ivan and Diklić, Aleksandar and Gavrović-Jankulović, Marija and Manojlović, Dragan D.",
year = "2020",
abstract = "The background level of lead (Pb) in drinking water in Serbia represents a significant health issue. The aim of study was to examine the content of significant toxic and essential trace metals (Mn, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U) in the thyroid tissues with diagnosed colloid goiter (CG). Different variables were investigated (sex, age, smoking habits, goiter size, and previous history of any thyroid disease). The study was designed to compare the goiter tissues (GTs) to sex- and age-matched healthy- and malignant-thyroid tissues (HTTs and MTTs, respectively). All trace metals were quantified by inductively coupled plasma mass spectrometry (ICP-MS). The reduced content of essential elements (Mn and Se) and increased content of toxic metals (Pb, Th, and U) were found in GTs compared with HTTs. The increased content of the same toxic metals separated GTs from MTTs, as well as reduced content of Mn and Cd. Our results indicated that Pb acts as the main goitrogen, which could highlight its role in the unknown etiology of CG disease. Pb/Se ratio could be considered as a relevant parameter for the tissue separation of GTs from HTTs and/or MTTs. This research also provides novel data on the effects of metal pollutants on thyroid health hazards. Graphical Abstract: [Figure not available: see fulltext.].",
journal = "Exposure and Health",
title = "Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease",
volume = "12",
number = "2",
pages = "255-264",
doi = "10.1007/s12403-019-00309-9"
}
Stojsavljević, A., Rovčanin, B., Krstić, Đ. D., Borković-Mitić, S. S., Paunović, I., Diklić, A., Gavrović-Jankulović, M.,& Manojlović, D. D.. (2020). Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease. in Exposure and Health, 12(2), 255-264.
https://doi.org/10.1007/s12403-019-00309-9
Stojsavljević A, Rovčanin B, Krstić ĐD, Borković-Mitić SS, Paunović I, Diklić A, Gavrović-Jankulović M, Manojlović DD. Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease. in Exposure and Health. 2020;12(2):255-264.
doi:10.1007/s12403-019-00309-9 .
Stojsavljević, Aleksandar, Rovčanin, Branislav, Krstić, Đurđa D., Borković-Mitić, Slavica S., Paunović, Ivan, Diklić, Aleksandar, Gavrović-Jankulović, Marija, Manojlović, Dragan D., "Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease" in Exposure and Health, 12, no. 2 (2020):255-264,
https://doi.org/10.1007/s12403-019-00309-9 . .
9
6
7

Significance of arsenic and lead in Hashimoto's thyroiditis demonstrated on thyroid tissue, blood, and urine samples

Stojsavljević, Aleksandar; Rovčanin, Branislav; Jagodić, Jovana; Drašković Radojković, Danijela; Paunović, Ivan; Gavrović-Jankulović, Marija; Manojlović, Dragan D.

(Elsevier, 2020)

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Jagodić, Jovana
AU  - Drašković Radojković, Danijela
AU  - Paunović, Ivan
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan D.
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4032
AB  - Our previous investigation showed significantly increased arsenic (As) content in thyroid tissue samples of patients with Hashimoto's thyroiditis (HT). This research aimed to extend previous findings and provide reliable insight into the close relationship between As and other trace elements with HT by considering a greater number of thyroid tissue samples, accompanied by blood and urine samples. The essential trace elements for thyroid homeostasis (Mn, Cu, Zn, Se) and the main threatening toxic trace elements (Ni, As, Pb, Cd, U) was analyzed by inductively coupled plasma-mass spectrometry (ICP-MS). Relevant parameters that could affect the concentration of trace elements were considered. This research showed that there was a difference in the elemental profile between HT and control samples. The most important findings were related to the elevated As and Pb content in the thyroid tissue and HT blood samples. The obtained negative correlations between As and Pb with Se may explain the antagonistic effect of As and Pb on the extrusion of essential Se from the HT tissue. The reduced Se content in the blood and its increased content in urine samples may further confirm this hypothesis and explain the lack of Se in HT. Furthermore, the reported results may highlight the unresolved molecular basis of HT and could indicate the role of trace element effects on thyroid homeostasis.
PB  - Elsevier
T2  - Environmental Research
T1  - Significance of arsenic and lead in Hashimoto's thyroiditis demonstrated on thyroid tissue, blood, and urine samples
VL  - 186
SP  - 109538
DO  - 10.1016/j.envres.2020.109538
ER  - 
@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Jagodić, Jovana and Drašković Radojković, Danijela and Paunović, Ivan and Gavrović-Jankulović, Marija and Manojlović, Dragan D.",
year = "2020",
abstract = "Our previous investigation showed significantly increased arsenic (As) content in thyroid tissue samples of patients with Hashimoto's thyroiditis (HT). This research aimed to extend previous findings and provide reliable insight into the close relationship between As and other trace elements with HT by considering a greater number of thyroid tissue samples, accompanied by blood and urine samples. The essential trace elements for thyroid homeostasis (Mn, Cu, Zn, Se) and the main threatening toxic trace elements (Ni, As, Pb, Cd, U) was analyzed by inductively coupled plasma-mass spectrometry (ICP-MS). Relevant parameters that could affect the concentration of trace elements were considered. This research showed that there was a difference in the elemental profile between HT and control samples. The most important findings were related to the elevated As and Pb content in the thyroid tissue and HT blood samples. The obtained negative correlations between As and Pb with Se may explain the antagonistic effect of As and Pb on the extrusion of essential Se from the HT tissue. The reduced Se content in the blood and its increased content in urine samples may further confirm this hypothesis and explain the lack of Se in HT. Furthermore, the reported results may highlight the unresolved molecular basis of HT and could indicate the role of trace element effects on thyroid homeostasis.",
publisher = "Elsevier",
journal = "Environmental Research",
title = "Significance of arsenic and lead in Hashimoto's thyroiditis demonstrated on thyroid tissue, blood, and urine samples",
volume = "186",
pages = "109538",
doi = "10.1016/j.envres.2020.109538"
}
Stojsavljević, A., Rovčanin, B., Jagodić, J., Drašković Radojković, D., Paunović, I., Gavrović-Jankulović, M.,& Manojlović, D. D.. (2020). Significance of arsenic and lead in Hashimoto's thyroiditis demonstrated on thyroid tissue, blood, and urine samples. in Environmental Research
Elsevier., 186, 109538.
https://doi.org/10.1016/j.envres.2020.109538
Stojsavljević A, Rovčanin B, Jagodić J, Drašković Radojković D, Paunović I, Gavrović-Jankulović M, Manojlović DD. Significance of arsenic and lead in Hashimoto's thyroiditis demonstrated on thyroid tissue, blood, and urine samples. in Environmental Research. 2020;186:109538.
doi:10.1016/j.envres.2020.109538 .
Stojsavljević, Aleksandar, Rovčanin, Branislav, Jagodić, Jovana, Drašković Radojković, Danijela, Paunović, Ivan, Gavrović-Jankulović, Marija, Manojlović, Dragan D., "Significance of arsenic and lead in Hashimoto's thyroiditis demonstrated on thyroid tissue, blood, and urine samples" in Environmental Research, 186 (2020):109538,
https://doi.org/10.1016/j.envres.2020.109538 . .
12
7
9

A real-life study of the efficacy of sublingual immunotherapy against weed allergies in the Serbian population

Tadić, D.; Popović, Milica M.; Gavrović-Jankulović, Marija; Đurić, Vojislav; Tomić-Špirić, Vesna; Rašković, Sanvila S.; Perić Popadić, Aleksandra

(Elsevier, 2019)

TY  - JOUR
AU  - Tadić, D.
AU  - Popović, Milica M.
AU  - Gavrović-Jankulović, Marija
AU  - Đurić, Vojislav
AU  - Tomić-Špirić, Vesna
AU  - Rašković, Sanvila S.
AU  - Perić Popadić, Aleksandra
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3728
AB  - Introduction: In the Serbian population allergy to weed pollen is the most common type of pollen-associated allergy, ahead of grass and tree pollens. Besides causing discomfort, allergy to pollen is often associated with rhinitis and asthma. Allergen-specific immunotherapy (ASIT) is the only treatment that can lead to potential long-term immune modification while reducing development of new sensitization and halting disease progression. The aim of the present study is to evaluate the efficacy of sublingual immunotherapy (SLIT) to weeds in the adult patient population using vaccine produced by the local Serbian Torlak Institute for virology, vaccines and serum. Methods: Adult patients with a clinical history of allergic rhinitis with and without asthma were included in the study. IgE-mediated sensitization to grass, tree and weed pollens was confirmed by skin prick testing and/or positive specific IgE. Patients were divided into two groups: patients with allergy to tree and grass pollen and patients with allergy to weeds. All patients received SLIT for three years, either with or without additional symptomatic therapy. Results: Three-year SLIT therapy led to significant improvement in several parameters, including skin-prick reactivity, decrease in specific IgE and use of symptomatic therapy, with mild adverse effects and high patient satisfaction concerning therapy. Conclusion: Three-year SLIT is a safe and efficient treatment option for respiratory allergy to weeds. Further observations in a larger number of patients could provide a better epidemiological evaluation of SLIT, but the positive effects we observed in our study may be considered representative despite the small number of patients.
PB  - Elsevier
T2  - Revue Francaise d'Allergologie
T1  - A real-life study of the efficacy of sublingual immunotherapy against weed allergies in the Serbian population
VL  - 59
IS  - 7
SP  - 474
EP  - 480
DO  - 10.1016/j.reval.2019.05.006
ER  - 
@article{
author = "Tadić, D. and Popović, Milica M. and Gavrović-Jankulović, Marija and Đurić, Vojislav and Tomić-Špirić, Vesna and Rašković, Sanvila S. and Perić Popadić, Aleksandra",
year = "2019",
abstract = "Introduction: In the Serbian population allergy to weed pollen is the most common type of pollen-associated allergy, ahead of grass and tree pollens. Besides causing discomfort, allergy to pollen is often associated with rhinitis and asthma. Allergen-specific immunotherapy (ASIT) is the only treatment that can lead to potential long-term immune modification while reducing development of new sensitization and halting disease progression. The aim of the present study is to evaluate the efficacy of sublingual immunotherapy (SLIT) to weeds in the adult patient population using vaccine produced by the local Serbian Torlak Institute for virology, vaccines and serum. Methods: Adult patients with a clinical history of allergic rhinitis with and without asthma were included in the study. IgE-mediated sensitization to grass, tree and weed pollens was confirmed by skin prick testing and/or positive specific IgE. Patients were divided into two groups: patients with allergy to tree and grass pollen and patients with allergy to weeds. All patients received SLIT for three years, either with or without additional symptomatic therapy. Results: Three-year SLIT therapy led to significant improvement in several parameters, including skin-prick reactivity, decrease in specific IgE and use of symptomatic therapy, with mild adverse effects and high patient satisfaction concerning therapy. Conclusion: Three-year SLIT is a safe and efficient treatment option for respiratory allergy to weeds. Further observations in a larger number of patients could provide a better epidemiological evaluation of SLIT, but the positive effects we observed in our study may be considered representative despite the small number of patients.",
publisher = "Elsevier",
journal = "Revue Francaise d'Allergologie",
title = "A real-life study of the efficacy of sublingual immunotherapy against weed allergies in the Serbian population",
volume = "59",
number = "7",
pages = "474-480",
doi = "10.1016/j.reval.2019.05.006"
}
Tadić, D., Popović, M. M., Gavrović-Jankulović, M., Đurić, V., Tomić-Špirić, V., Rašković, S. S.,& Perić Popadić, A.. (2019). A real-life study of the efficacy of sublingual immunotherapy against weed allergies in the Serbian population. in Revue Francaise d'Allergologie
Elsevier., 59(7), 474-480.
https://doi.org/10.1016/j.reval.2019.05.006
Tadić D, Popović MM, Gavrović-Jankulović M, Đurić V, Tomić-Špirić V, Rašković SS, Perić Popadić A. A real-life study of the efficacy of sublingual immunotherapy against weed allergies in the Serbian population. in Revue Francaise d'Allergologie. 2019;59(7):474-480.
doi:10.1016/j.reval.2019.05.006 .
Tadić, D., Popović, Milica M., Gavrović-Jankulović, Marija, Đurić, Vojislav, Tomić-Špirić, Vesna, Rašković, Sanvila S., Perić Popadić, Aleksandra, "A real-life study of the efficacy of sublingual immunotherapy against weed allergies in the Serbian population" in Revue Francaise d'Allergologie, 59, no. 7 (2019):474-480,
https://doi.org/10.1016/j.reval.2019.05.006 . .

The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines

Nešić, Andrijana N.; Čavić, Milena; Popović, Milica M.; Zlatanova, Milena; Pieters, Raymond; Smit, Joost; Gavrović-Jankulović, Marija

(MDPI, 2019)

TY  - JOUR
AU  - Nešić, Andrijana N.
AU  - Čavić, Milena
AU  - Popović, Milica M.
AU  - Zlatanova, Milena
AU  - Pieters, Raymond
AU  - Smit, Joost
AU  - Gavrović-Jankulović, Marija
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3799
AB  - Impairment of the intestinal barrier is one of the key events in the initiation of the sensitization process in food allergy. The aim of this study was to explore the effects of kiwifruit allergen Act d 1 on intestinal permeability and tight junction protein (TJP) gene expression in vivo and to explore its potential to activate the NF-ĸB signaling pathway and to regulate expression of epithelial pro-allergenic cytokines. Influences of Act d 1 on TJP gene expression and pro-allergenic cytokines in the mouse intestine was analyzed by qPCR upon allergen administration by oral gavage. The effect on the in vivo intestinal permeability was assessed in ELISA by measuring the translocation of β-lactoglobulin (BLG) into circulation. The capacity of Act d 1 to activate the NF-ĸB pathway was tested in HEK293 cells by fluorescent microscopy and flow cytometry. Administration of Actinidin (Act d 1) increased intestinal permeability to the BLG. This was accompanied by changes in gene expression of TJP mRNA and pro-allergenic cytokines IL-25, IL-33, and thymic stromal lymphopoietin (TSLP) compared to the control. Act d 1 reduced TEER of the HEK293 monolayer, was positive in an NF-ĸB-reporter HEK293 cell assay, and induced secretion of TSLP. These findings shed more light on the molecular events in the sensitization process of kiwifruit but possibly also of other protease food allergens.
PB  - MDPI
T2  - Biomolecules
T1  - The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines
VL  - 9
IS  - 12
SP  - 816
DO  - 10.3390/biom9120816
ER  - 
@article{
author = "Nešić, Andrijana N. and Čavić, Milena and Popović, Milica M. and Zlatanova, Milena and Pieters, Raymond and Smit, Joost and Gavrović-Jankulović, Marija",
year = "2019",
abstract = "Impairment of the intestinal barrier is one of the key events in the initiation of the sensitization process in food allergy. The aim of this study was to explore the effects of kiwifruit allergen Act d 1 on intestinal permeability and tight junction protein (TJP) gene expression in vivo and to explore its potential to activate the NF-ĸB signaling pathway and to regulate expression of epithelial pro-allergenic cytokines. Influences of Act d 1 on TJP gene expression and pro-allergenic cytokines in the mouse intestine was analyzed by qPCR upon allergen administration by oral gavage. The effect on the in vivo intestinal permeability was assessed in ELISA by measuring the translocation of β-lactoglobulin (BLG) into circulation. The capacity of Act d 1 to activate the NF-ĸB pathway was tested in HEK293 cells by fluorescent microscopy and flow cytometry. Administration of Actinidin (Act d 1) increased intestinal permeability to the BLG. This was accompanied by changes in gene expression of TJP mRNA and pro-allergenic cytokines IL-25, IL-33, and thymic stromal lymphopoietin (TSLP) compared to the control. Act d 1 reduced TEER of the HEK293 monolayer, was positive in an NF-ĸB-reporter HEK293 cell assay, and induced secretion of TSLP. These findings shed more light on the molecular events in the sensitization process of kiwifruit but possibly also of other protease food allergens.",
publisher = "MDPI",
journal = "Biomolecules",
title = "The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines",
volume = "9",
number = "12",
pages = "816",
doi = "10.3390/biom9120816"
}
Nešić, A. N., Čavić, M., Popović, M. M., Zlatanova, M., Pieters, R., Smit, J.,& Gavrović-Jankulović, M.. (2019). The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines. in Biomolecules
MDPI., 9(12), 816.
https://doi.org/10.3390/biom9120816
Nešić AN, Čavić M, Popović MM, Zlatanova M, Pieters R, Smit J, Gavrović-Jankulović M. The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines. in Biomolecules. 2019;9(12):816.
doi:10.3390/biom9120816 .
Nešić, Andrijana N., Čavić, Milena, Popović, Milica M., Zlatanova, Milena, Pieters, Raymond, Smit, Joost, Gavrović-Jankulović, Marija, "The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines" in Biomolecules, 9, no. 12 (2019):816,
https://doi.org/10.3390/biom9120816 . .
1
4
1
4

Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology

Stojsavljević, Aleksandar; Rovčanin, Branislav; Krstić, Đurđa D.; Jagodić, Jovana; Borković-Mitić, Slavica S.; Paunović, Ivan; Živaljević, Vladan; Mitić, Bojan M.; Gavrović-Jankulović, Marija; Manojlović, Dragan D.

(Elsevier, 2019)

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa D.
AU  - Jagodić, Jovana
AU  - Borković-Mitić, Slavica S.
AU  - Paunović, Ivan
AU  - Živaljević, Vladan
AU  - Mitić, Bojan M.
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan D.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3293
AB  - Background: The etiology of papillary thyroid carcinoma (PTC) is unknown and some literature data support the hypothesis that heavy metals, as endocrine disrupters, could play a major role in the pathogenesis of thyroid cancer. This study aimed to estimate the content of selected toxic and essential trace metals (Mn, Co, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U), as well as the selected ratio's (Cu/Zn and Cd/Se) in the malignant thyroid tissues according to sex, age, smoking habits, familial history of any thyroid disease, pathohistological (PH) types of PTC, tumor size, the existence of a thyroid capsular invasion, intrathyroid tumor dissemination, retrosternal thyroid growth, and TNM progress of PTC. Methods: The study included 66 patients with PTC (women/men ratio = 46/20, mean age: 54 ± 14 years). A comparative analysis was made by collecting the healthy thyroid tissues (HTTs) of the same patients, making the total number of samples 132. All trace metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). Results: Metals that significantly separated papillary thyroid tissues (PTTs) from the HTTs were Cd, U and Se (p < 0.05). The obtained negative correlation between Cd and Se in the PTTs could explain extrusion of essential Se caused by increased content of Cd. Only Cd had an influence on the retrosternal thyroid growth, while the essential metals (Mn, Co, and Zn) had an influence on thyroid capsular invasion. Conclusion: It was found that Cd act as the main endocrine disrupter, which could highlight its role in the etiology of PTC. Considering that the Cd/Se ratio significantly separated two studied groups and had an influence on the retrosternal thyroid growth, its altered content could contribute to the better understanding of the molecular basis for pathophysiological changes in the PTC.
PB  - Elsevier
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology
VL  - 55
SP  - 190
EP  - 195
DO  - 10.1016/j.jtemb.2019.06.009
ER  - 
@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa D. and Jagodić, Jovana and Borković-Mitić, Slavica S. and Paunović, Ivan and Živaljević, Vladan and Mitić, Bojan M. and Gavrović-Jankulović, Marija and Manojlović, Dragan D.",
year = "2019",
abstract = "Background: The etiology of papillary thyroid carcinoma (PTC) is unknown and some literature data support the hypothesis that heavy metals, as endocrine disrupters, could play a major role in the pathogenesis of thyroid cancer. This study aimed to estimate the content of selected toxic and essential trace metals (Mn, Co, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U), as well as the selected ratio's (Cu/Zn and Cd/Se) in the malignant thyroid tissues according to sex, age, smoking habits, familial history of any thyroid disease, pathohistological (PH) types of PTC, tumor size, the existence of a thyroid capsular invasion, intrathyroid tumor dissemination, retrosternal thyroid growth, and TNM progress of PTC. Methods: The study included 66 patients with PTC (women/men ratio = 46/20, mean age: 54 ± 14 years). A comparative analysis was made by collecting the healthy thyroid tissues (HTTs) of the same patients, making the total number of samples 132. All trace metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). Results: Metals that significantly separated papillary thyroid tissues (PTTs) from the HTTs were Cd, U and Se (p < 0.05). The obtained negative correlation between Cd and Se in the PTTs could explain extrusion of essential Se caused by increased content of Cd. Only Cd had an influence on the retrosternal thyroid growth, while the essential metals (Mn, Co, and Zn) had an influence on thyroid capsular invasion. Conclusion: It was found that Cd act as the main endocrine disrupter, which could highlight its role in the etiology of PTC. Considering that the Cd/Se ratio significantly separated two studied groups and had an influence on the retrosternal thyroid growth, its altered content could contribute to the better understanding of the molecular basis for pathophysiological changes in the PTC.",
publisher = "Elsevier",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology",
volume = "55",
pages = "190-195",
doi = "10.1016/j.jtemb.2019.06.009"
}
Stojsavljević, A., Rovčanin, B., Krstić, Đ. D., Jagodić, J., Borković-Mitić, S. S., Paunović, I., Živaljević, V., Mitić, B. M., Gavrović-Jankulović, M.,& Manojlović, D. D.. (2019). Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology. in Journal of Trace Elements in Medicine and Biology
Elsevier., 55, 190-195.
https://doi.org/10.1016/j.jtemb.2019.06.009
Stojsavljević A, Rovčanin B, Krstić ĐD, Jagodić J, Borković-Mitić SS, Paunović I, Živaljević V, Mitić BM, Gavrović-Jankulović M, Manojlović DD. Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology. in Journal of Trace Elements in Medicine and Biology. 2019;55:190-195.
doi:10.1016/j.jtemb.2019.06.009 .
Stojsavljević, Aleksandar, Rovčanin, Branislav, Krstić, Đurđa D., Jagodić, Jovana, Borković-Mitić, Slavica S., Paunović, Ivan, Živaljević, Vladan, Mitić, Bojan M., Gavrović-Jankulović, Marija, Manojlović, Dragan D., "Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology" in Journal of Trace Elements in Medicine and Biology, 55 (2019):190-195,
https://doi.org/10.1016/j.jtemb.2019.06.009 . .
1
14
14
15

Supplementary material for the article: Stojsavljević, A.; Rovčanin, B.; Krstić; Jagodić, J.; Borković-Mitić, S.; Paunović, I.; Živaljević, V.; Mitić, B.; Gavrović-Jankulović, M.; Manojlović, D. Cadmium as Main Endocrine Disruptor in Papillary Thyroid Carcinoma and the Significance of Cd/Se Ratio for Thyroid Tissue Pathophysiology. Journal of Trace Elements in Medicine and Biology 2019, 55, 190–195. https://doi.org/10.1016/j.jtemb.2019.06.009

Stojsavljević, Aleksandar; Rovčanin, Branislav; Krstić, Đurđa D.; Jagodić, Jovana; Borković-Mitić, Slavica S.; Paunović, Ivan; Živaljević, Vladan; Mitić, Bojan M.; Gavrović-Jankulović, Marija; Manojlović, Dragan D.

(Elsevier, 2019)

TY  - DATA
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa D.
AU  - Jagodić, Jovana
AU  - Borković-Mitić, Slavica S.
AU  - Paunović, Ivan
AU  - Živaljević, Vladan
AU  - Mitić, Bojan M.
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan D.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3294
PB  - Elsevier
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Supplementary material for the article: Stojsavljević, A.; Rovčanin, B.; Krstić; Jagodić, J.; Borković-Mitić, S.; Paunović, I.; Živaljević, V.; Mitić, B.; Gavrović-Jankulović, M.; Manojlović, D. Cadmium as Main Endocrine Disruptor in Papillary Thyroid Carcinoma and the Significance of Cd/Se Ratio for Thyroid Tissue Pathophysiology. Journal of Trace Elements in Medicine and Biology 2019, 55, 190–195. https://doi.org/10.1016/j.jtemb.2019.06.009
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3294
ER  - 
@misc{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa D. and Jagodić, Jovana and Borković-Mitić, Slavica S. and Paunović, Ivan and Živaljević, Vladan and Mitić, Bojan M. and Gavrović-Jankulović, Marija and Manojlović, Dragan D.",
year = "2019",
publisher = "Elsevier",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Supplementary material for the article: Stojsavljević, A.; Rovčanin, B.; Krstić; Jagodić, J.; Borković-Mitić, S.; Paunović, I.; Živaljević, V.; Mitić, B.; Gavrović-Jankulović, M.; Manojlović, D. Cadmium as Main Endocrine Disruptor in Papillary Thyroid Carcinoma and the Significance of Cd/Se Ratio for Thyroid Tissue Pathophysiology. Journal of Trace Elements in Medicine and Biology 2019, 55, 190–195. https://doi.org/10.1016/j.jtemb.2019.06.009",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3294"
}
Stojsavljević, A., Rovčanin, B., Krstić, Đ. D., Jagodić, J., Borković-Mitić, S. S., Paunović, I., Živaljević, V., Mitić, B. M., Gavrović-Jankulović, M.,& Manojlović, D. D.. (2019). Supplementary material for the article: Stojsavljević, A.; Rovčanin, B.; Krstić; Jagodić, J.; Borković-Mitić, S.; Paunović, I.; Živaljević, V.; Mitić, B.; Gavrović-Jankulović, M.; Manojlović, D. Cadmium as Main Endocrine Disruptor in Papillary Thyroid Carcinoma and the Significance of Cd/Se Ratio for Thyroid Tissue Pathophysiology. Journal of Trace Elements in Medicine and Biology 2019, 55, 190–195. https://doi.org/10.1016/j.jtemb.2019.06.009. in Journal of Trace Elements in Medicine and Biology
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_3294
Stojsavljević A, Rovčanin B, Krstić ĐD, Jagodić J, Borković-Mitić SS, Paunović I, Živaljević V, Mitić BM, Gavrović-Jankulović M, Manojlović DD. Supplementary material for the article: Stojsavljević, A.; Rovčanin, B.; Krstić; Jagodić, J.; Borković-Mitić, S.; Paunović, I.; Živaljević, V.; Mitić, B.; Gavrović-Jankulović, M.; Manojlović, D. Cadmium as Main Endocrine Disruptor in Papillary Thyroid Carcinoma and the Significance of Cd/Se Ratio for Thyroid Tissue Pathophysiology. Journal of Trace Elements in Medicine and Biology 2019, 55, 190–195. https://doi.org/10.1016/j.jtemb.2019.06.009. in Journal of Trace Elements in Medicine and Biology. 2019;.
https://hdl.handle.net/21.15107/rcub_cherry_3294 .
Stojsavljević, Aleksandar, Rovčanin, Branislav, Krstić, Đurđa D., Jagodić, Jovana, Borković-Mitić, Slavica S., Paunović, Ivan, Živaljević, Vladan, Mitić, Bojan M., Gavrović-Jankulović, Marija, Manojlović, Dragan D., "Supplementary material for the article: Stojsavljević, A.; Rovčanin, B.; Krstić; Jagodić, J.; Borković-Mitić, S.; Paunović, I.; Živaljević, V.; Mitić, B.; Gavrović-Jankulović, M.; Manojlović, D. Cadmium as Main Endocrine Disruptor in Papillary Thyroid Carcinoma and the Significance of Cd/Se Ratio for Thyroid Tissue Pathophysiology. Journal of Trace Elements in Medicine and Biology 2019, 55, 190–195. https://doi.org/10.1016/j.jtemb.2019.06.009" in Journal of Trace Elements in Medicine and Biology (2019),
https://hdl.handle.net/21.15107/rcub_cherry_3294 .

Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases

Stojsavljević, Aleksandar; Rovčanin, Branislav; Krstić, Đurđa D.; Borković-Mitić, Slavica S.; Paunović, Ivan; Kodranov, Igor D.; Gavrović-Jankulović, Marija; Manojlović, Dragan D.

(Elsevier, 2019)

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa D.
AU  - Borković-Mitić, Slavica S.
AU  - Paunović, Ivan
AU  - Kodranov, Igor D.
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan D.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3285
AB  - Evaluation of trace metals at level of solid tissue can provide better information than blood or urine and, therefore, could highlight the role of metals in the etiology of organ-specific disease. The current study aimed to establish the baseline content of four essential (Mn, Cu, Zn, Se) and four toxic metals (As, Cd, Pb, U) in the healthy thyroid tissues (HTTs) by considering sex, age and smoking habits. A further aim was to examine whether differences in the content of metals exist in regard to the thyroid diseases, such as benign tumor (BT), Hashimoto's thyroiditis (HT), multinodular goiter (MNG) and thyroid cancer (TC). A total number of investigated tissue samples were 423. All metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). It was found that the content of Cu and U was higher in HTTs of women, while the content of Zn was higher in HTTs of men. Increased content of Zn and decreased content of U was found in the group of HTTs above 50 years compared to a younger group (<50 years). Increased content of Cd, Pb and U distinguish smokers from the non-smokers. In comparison with other population groups worldwide, investigated Serbian population had up to 15 times reduced content of Se. Despite the difference in metal's profile according to biological variables, this study also demonstrated, for the first time, that each thyroid disease has its unique metal's profile. The most altered metal's content was found in tissues with HT. Contrarily, the greatest similarity in metal's content with HTTs was found in BT tissues. Based on the increased content, metal's that dominantly discriminated HTTs from the HT, MNG and TC was As, Pb and Cd, respectively. Reported results could highlight the role of toxic and essential trace metals in the not very well clarified etiology of thyroid diseases and, moreover, could provide a molecular basis for pathophysiological changes of metal's hazardous effects on thyroid health at the tissue level.
PB  - Elsevier
T2  - Ecotoxicology and Environmental Safety
T1  - Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases
VL  - 183
DO  - 10.1016/j.ecoenv.2019.109479
ER  - 
@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa D. and Borković-Mitić, Slavica S. and Paunović, Ivan and Kodranov, Igor D. and Gavrović-Jankulović, Marija and Manojlović, Dragan D.",
year = "2019",
abstract = "Evaluation of trace metals at level of solid tissue can provide better information than blood or urine and, therefore, could highlight the role of metals in the etiology of organ-specific disease. The current study aimed to establish the baseline content of four essential (Mn, Cu, Zn, Se) and four toxic metals (As, Cd, Pb, U) in the healthy thyroid tissues (HTTs) by considering sex, age and smoking habits. A further aim was to examine whether differences in the content of metals exist in regard to the thyroid diseases, such as benign tumor (BT), Hashimoto's thyroiditis (HT), multinodular goiter (MNG) and thyroid cancer (TC). A total number of investigated tissue samples were 423. All metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). It was found that the content of Cu and U was higher in HTTs of women, while the content of Zn was higher in HTTs of men. Increased content of Zn and decreased content of U was found in the group of HTTs above 50 years compared to a younger group (<50 years). Increased content of Cd, Pb and U distinguish smokers from the non-smokers. In comparison with other population groups worldwide, investigated Serbian population had up to 15 times reduced content of Se. Despite the difference in metal's profile according to biological variables, this study also demonstrated, for the first time, that each thyroid disease has its unique metal's profile. The most altered metal's content was found in tissues with HT. Contrarily, the greatest similarity in metal's content with HTTs was found in BT tissues. Based on the increased content, metal's that dominantly discriminated HTTs from the HT, MNG and TC was As, Pb and Cd, respectively. Reported results could highlight the role of toxic and essential trace metals in the not very well clarified etiology of thyroid diseases and, moreover, could provide a molecular basis for pathophysiological changes of metal's hazardous effects on thyroid health at the tissue level.",
publisher = "Elsevier",
journal = "Ecotoxicology and Environmental Safety",
title = "Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases",
volume = "183",
doi = "10.1016/j.ecoenv.2019.109479"
}
Stojsavljević, A., Rovčanin, B., Krstić, Đ. D., Borković-Mitić, S. S., Paunović, I., Kodranov, I. D., Gavrović-Jankulović, M.,& Manojlović, D. D.. (2019). Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases. in Ecotoxicology and Environmental Safety
Elsevier., 183.
https://doi.org/10.1016/j.ecoenv.2019.109479
Stojsavljević A, Rovčanin B, Krstić ĐD, Borković-Mitić SS, Paunović I, Kodranov ID, Gavrović-Jankulović M, Manojlović DD. Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases. in Ecotoxicology and Environmental Safety. 2019;183.
doi:10.1016/j.ecoenv.2019.109479 .
Stojsavljević, Aleksandar, Rovčanin, Branislav, Krstić, Đurđa D., Borković-Mitić, Slavica S., Paunović, Ivan, Kodranov, Igor D., Gavrović-Jankulović, Marija, Manojlović, Dragan D., "Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases" in Ecotoxicology and Environmental Safety, 183 (2019),
https://doi.org/10.1016/j.ecoenv.2019.109479 . .
1
22
16
19

Supplementary data for the article: Stojsavljević, A.; Rovčanin, B.; Krstić, Đ.; Borković-Mitić, S.; Paunović, I.; Kodranov, I.; Gavrović-Jankulović, M.; Manojlović, D. Evaluation of Trace Metals in Thyroid Tissues: Comparative Analysis with Benign and Malignant Thyroid Diseases. Ecotoxicology and Environmental Safety 2019, 183. https://doi.org/10.1016/j.ecoenv.2019.109479

Stojsavljević, Aleksandar; Rovčanin, Branislav; Krstić, Đurđa D.; Borković-Mitić, Slavica S.; Paunović, Ivan; Kodranov, Igor D.; Gavrović-Jankulović, Marija; Manojlović, Dragan D.

(Elsevier, 2019)

TY  - DATA
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa D.
AU  - Borković-Mitić, Slavica S.
AU  - Paunović, Ivan
AU  - Kodranov, Igor D.
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan D.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3286
PB  - Elsevier
T2  - Ecotoxicology and Environmental Safety
T1  - Supplementary data for the article: Stojsavljević, A.; Rovčanin, B.; Krstić, Đ.; Borković-Mitić, S.; Paunović, I.; Kodranov, I.; Gavrović-Jankulović, M.; Manojlović, D. Evaluation of  Trace Metals in Thyroid Tissues: Comparative Analysis with Benign and Malignant Thyroid Diseases. Ecotoxicology and Environmental Safety 2019, 183. https://doi.org/10.1016/j.ecoenv.2019.109479
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3286
ER  - 
@misc{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa D. and Borković-Mitić, Slavica S. and Paunović, Ivan and Kodranov, Igor D. and Gavrović-Jankulović, Marija and Manojlović, Dragan D.",
year = "2019",
publisher = "Elsevier",
journal = "Ecotoxicology and Environmental Safety",
title = "Supplementary data for the article: Stojsavljević, A.; Rovčanin, B.; Krstić, Đ.; Borković-Mitić, S.; Paunović, I.; Kodranov, I.; Gavrović-Jankulović, M.; Manojlović, D. Evaluation of  Trace Metals in Thyroid Tissues: Comparative Analysis with Benign and Malignant Thyroid Diseases. Ecotoxicology and Environmental Safety 2019, 183. https://doi.org/10.1016/j.ecoenv.2019.109479",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3286"
}
Stojsavljević, A., Rovčanin, B., Krstić, Đ. D., Borković-Mitić, S. S., Paunović, I., Kodranov, I. D., Gavrović-Jankulović, M.,& Manojlović, D. D.. (2019). Supplementary data for the article: Stojsavljević, A.; Rovčanin, B.; Krstić, Đ.; Borković-Mitić, S.; Paunović, I.; Kodranov, I.; Gavrović-Jankulović, M.; Manojlović, D. Evaluation of  Trace Metals in Thyroid Tissues: Comparative Analysis with Benign and Malignant Thyroid Diseases. Ecotoxicology and Environmental Safety 2019, 183. https://doi.org/10.1016/j.ecoenv.2019.109479. in Ecotoxicology and Environmental Safety
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_3286
Stojsavljević A, Rovčanin B, Krstić ĐD, Borković-Mitić SS, Paunović I, Kodranov ID, Gavrović-Jankulović M, Manojlović DD. Supplementary data for the article: Stojsavljević, A.; Rovčanin, B.; Krstić, Đ.; Borković-Mitić, S.; Paunović, I.; Kodranov, I.; Gavrović-Jankulović, M.; Manojlović, D. Evaluation of  Trace Metals in Thyroid Tissues: Comparative Analysis with Benign and Malignant Thyroid Diseases. Ecotoxicology and Environmental Safety 2019, 183. https://doi.org/10.1016/j.ecoenv.2019.109479. in Ecotoxicology and Environmental Safety. 2019;.
https://hdl.handle.net/21.15107/rcub_cherry_3286 .
Stojsavljević, Aleksandar, Rovčanin, Branislav, Krstić, Đurđa D., Borković-Mitić, Slavica S., Paunović, Ivan, Kodranov, Igor D., Gavrović-Jankulović, Marija, Manojlović, Dragan D., "Supplementary data for the article: Stojsavljević, A.; Rovčanin, B.; Krstić, Đ.; Borković-Mitić, S.; Paunović, I.; Kodranov, I.; Gavrović-Jankulović, M.; Manojlović, D. Evaluation of  Trace Metals in Thyroid Tissues: Comparative Analysis with Benign and Malignant Thyroid Diseases. Ecotoxicology and Environmental Safety 2019, 183. https://doi.org/10.1016/j.ecoenv.2019.109479" in Ecotoxicology and Environmental Safety (2019),
https://hdl.handle.net/21.15107/rcub_cherry_3286 .

Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model

Nešić, Andrijana N.; Stam, Annemarie; Čavić, Milena; Ten Klooster, Jean Paul; Pieters, Raymond; Smit, Joost; Gavrović-Jankulović, Marija

(2019)

TY  - JOUR
AU  - Nešić, Andrijana N.
AU  - Stam, Annemarie
AU  - Čavić, Milena
AU  - Ten Klooster, Jean Paul
AU  - Pieters, Raymond
AU  - Smit, Joost
AU  - Gavrović-Jankulović, Marija
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3485
AB  - In this study, two intestinal models were employed to assess the modulatory potential of a major kiwifruit allergen on the innate immunity of epithelial cells. Effects of Act d 1 were analyzed in terms of gene expression and structural changes of tight junction (TJ) proteins, as well as up-regulation of pro-inflammatory cytokines in Caco-2 cells and, for the first time, in mouse-derived intestinal 2-dimensional (2D) organoids.

Biologically active Act d 1 induced up-regulation of TJ genes for CLDN-2, CLDN-3, CLDN-4, ZO-1, and on the protein level induced release of pro-inflammatory cytokines IL-1β, TNFα and IL-33 in both employed model systems. In 2D-organoids, active Act d 1 impaired the TJ protein networks of E-cadherin, claudin-3, and ZO-1.

2D-organoids generated from mouse intestine are a promising new model system for the assessment of allergen-induced intestinal cell responses and a useful tool for mitigation of risks associated with novel food proteins.
T2  - Journal of Functional Foods
T1  - Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model
VL  - 62
DO  - 10.1016/j.jff.2019.103556
ER  - 
@article{
author = "Nešić, Andrijana N. and Stam, Annemarie and Čavić, Milena and Ten Klooster, Jean Paul and Pieters, Raymond and Smit, Joost and Gavrović-Jankulović, Marija",
year = "2019",
abstract = "In this study, two intestinal models were employed to assess the modulatory potential of a major kiwifruit allergen on the innate immunity of epithelial cells. Effects of Act d 1 were analyzed in terms of gene expression and structural changes of tight junction (TJ) proteins, as well as up-regulation of pro-inflammatory cytokines in Caco-2 cells and, for the first time, in mouse-derived intestinal 2-dimensional (2D) organoids.

Biologically active Act d 1 induced up-regulation of TJ genes for CLDN-2, CLDN-3, CLDN-4, ZO-1, and on the protein level induced release of pro-inflammatory cytokines IL-1β, TNFα and IL-33 in both employed model systems. In 2D-organoids, active Act d 1 impaired the TJ protein networks of E-cadherin, claudin-3, and ZO-1.

2D-organoids generated from mouse intestine are a promising new model system for the assessment of allergen-induced intestinal cell responses and a useful tool for mitigation of risks associated with novel food proteins.",
journal = "Journal of Functional Foods",
title = "Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model",
volume = "62",
doi = "10.1016/j.jff.2019.103556"
}
Nešić, A. N., Stam, A., Čavić, M., Ten Klooster, J. P., Pieters, R., Smit, J.,& Gavrović-Jankulović, M.. (2019). Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model. in Journal of Functional Foods, 62.
https://doi.org/10.1016/j.jff.2019.103556
Nešić AN, Stam A, Čavić M, Ten Klooster JP, Pieters R, Smit J, Gavrović-Jankulović M. Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model. in Journal of Functional Foods. 2019;62.
doi:10.1016/j.jff.2019.103556 .
Nešić, Andrijana N., Stam, Annemarie, Čavić, Milena, Ten Klooster, Jean Paul, Pieters, Raymond, Smit, Joost, Gavrović-Jankulović, Marija, "Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model" in Journal of Functional Foods, 62 (2019),
https://doi.org/10.1016/j.jff.2019.103556 . .
3
3
3