TY  - JOUR
AU  - Nedić, Olgica
AU  - Penezić, Ana
AU  - Minić, Simeon
AU  - Radomirović, Mirjana Ž.
AU  - Nikolić, Milan
AU  - Ćirković-Veličković, Tanja
AU  - Gligorijević, Nikola
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6028
AB  - Common to all biological systems and living organisms are molecular interactions, which
may lead to specific physiological events. Most often, a cascade of events occurs, establishing an equilibrium between possibly competing and/or synergistic processes. Biochemical pathways that sustain life depend on multiple intrinsic and extrinsic factors contributing to aging and/or diseases. This article deals with food antioxidants and human proteins from the circulation, their interaction, their effect on the structure, properties, and function of antioxidant-bound proteins, and the possible impact of complex formation on antioxidants. An overview of studies examining interactions between individual antioxidant compounds and major blood proteins is presented with findings. Investigating antioxidant/protein interactions at the level of the human organism and determining antioxidant distribution between proteins and involvement in the particular physiological role is a very complex and challenging task. However, by knowing the role of a particular protein in certain pathology or aging, and the effect exerted by a particular antioxidant bound to it, it is possible to recommend specific food intake or resistance to it to improve the condition or slow down the process.
PB  - MDPI
T2  - Antioxidants
T1  - Food Antioxidants and Their Interaction with Human Proteins
VL  - 12
IS  - 4
SP  - 815
DO  - 10.3390/antiox12040815
ER  - 

@article{
author = "Nedić, Olgica and Penezić, Ana and Minić, Simeon and Radomirović, Mirjana Ž. and Nikolić, Milan and Ćirković-Veličković, Tanja and Gligorijević, Nikola",
year = "2023",
abstract = "Common to all biological systems and living organisms are molecular interactions, which
may lead to specific physiological events. Most often, a cascade of events occurs, establishing an equilibrium between possibly competing and/or synergistic processes. Biochemical pathways that sustain life depend on multiple intrinsic and extrinsic factors contributing to aging and/or diseases. This article deals with food antioxidants and human proteins from the circulation, their interaction, their effect on the structure, properties, and function of antioxidant-bound proteins, and the possible impact of complex formation on antioxidants. An overview of studies examining interactions between individual antioxidant compounds and major blood proteins is presented with findings. Investigating antioxidant/protein interactions at the level of the human organism and determining antioxidant distribution between proteins and involvement in the particular physiological role is a very complex and challenging task. However, by knowing the role of a particular protein in certain pathology or aging, and the effect exerted by a particular antioxidant bound to it, it is possible to recommend specific food intake or resistance to it to improve the condition or slow down the process.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Food Antioxidants and Their Interaction with Human Proteins",
volume = "12",
number = "4",
pages = "815",
doi = "10.3390/antiox12040815"
}

Nedić, O., Penezić, A., Minić, S., Radomirović, M. Ž., Nikolić, M., Ćirković-Veličković, T.,& Gligorijević, N.. (2023). Food Antioxidants and Their Interaction with Human Proteins. in Antioxidants
MDPI., 12(4), 815.
https://doi.org/10.3390/antiox12040815

Nedić O, Penezić A, Minić S, Radomirović MŽ, Nikolić M, Ćirković-Veličković T, Gligorijević N. Food Antioxidants and Their Interaction with Human Proteins. in Antioxidants. 2023;12(4):815.
doi:10.3390/antiox12040815 .

Nedić, Olgica, Penezić, Ana, Minić, Simeon, Radomirović, Mirjana Ž., Nikolić, Milan, Ćirković-Veličković, Tanja, Gligorijević, Nikola, "Food Antioxidants and Their Interaction with Human Proteins" in Antioxidants, 12, no. 4 (2023):815,
https://doi.org/10.3390/antiox12040815 . .

TY  - CONF
AU  - Nedić, Olgica
AU  - Gligorijević, Nikola
AU  - Penezić, Ana
AU  - Minić, Simeon
AU  - Radomirović, Mirjana Ž.
AU  - Nikolić, Milan
AU  - Ćirković-Veličković, Tanja
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6031
AB  - Our research work was focused on interactions between resveratrol (R) and fibrinogen (I), (dihydro)alpha-lipoic acid (ALA) and fibrinogen or albumin, and phycocyanobilin (PCB) and catalase. Resveratrol is found in grapes and berries, leafy greens are a source of ALA and alga Spirulina is a source of PCB. L-P interactions were investigated by following-up structural changes of proteins and/or ligands using spectrometric methods (spectrofluorimetry, CD, FTIR) and by examining the primary role of individual proteins upon ligand binding.
PB  - Belgrade : Faculty of Agriculture
C3  - 1st European Symposium on Phytochemicals in Medicine and Food, 7th-9th September, 2022. In: Book of Abstracts
T1  - Food antioxidants and their interaction with human proteins
SP  - 13
EP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6031
ER  - 

@conference{
author = "Nedić, Olgica and Gligorijević, Nikola and Penezić, Ana and Minić, Simeon and Radomirović, Mirjana Ž. and Nikolić, Milan and Ćirković-Veličković, Tanja",
year = "2022",
abstract = "Our research work was focused on interactions between resveratrol (R) and fibrinogen (I), (dihydro)alpha-lipoic acid (ALA) and fibrinogen or albumin, and phycocyanobilin (PCB) and catalase. Resveratrol is found in grapes and berries, leafy greens are a source of ALA and alga Spirulina is a source of PCB. L-P interactions were investigated by following-up structural changes of proteins and/or ligands using spectrometric methods (spectrofluorimetry, CD, FTIR) and by examining the primary role of individual proteins upon ligand binding.",
publisher = "Belgrade : Faculty of Agriculture",
journal = "1st European Symposium on Phytochemicals in Medicine and Food, 7th-9th September, 2022. In: Book of Abstracts",
title = "Food antioxidants and their interaction with human proteins",
pages = "13-13",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6031"
}

Nedić, O., Gligorijević, N., Penezić, A., Minić, S., Radomirović, M. Ž., Nikolić, M.,& Ćirković-Veličković, T.. (2022). Food antioxidants and their interaction with human proteins. in 1st European Symposium on Phytochemicals in Medicine and Food, 7th-9th September, 2022. In: Book of Abstracts
Belgrade : Faculty of Agriculture., 13-13.
https://hdl.handle.net/21.15107/rcub_cherry_6031

Nedić O, Gligorijević N, Penezić A, Minić S, Radomirović MŽ, Nikolić M, Ćirković-Veličković T. Food antioxidants and their interaction with human proteins. in 1st European Symposium on Phytochemicals in Medicine and Food, 7th-9th September, 2022. In: Book of Abstracts. 2022;:13-13.
https://hdl.handle.net/21.15107/rcub_cherry_6031 .

Nedić, Olgica, Gligorijević, Nikola, Penezić, Ana, Minić, Simeon, Radomirović, Mirjana Ž., Nikolić, Milan, Ćirković-Veličković, Tanja, "Food antioxidants and their interaction with human proteins" in 1st European Symposium on Phytochemicals in Medicine and Food, 7th-9th September, 2022. In: Book of Abstracts (2022):13-13,
https://hdl.handle.net/21.15107/rcub_cherry_6031 .

TY  - CONF
AU  - Gligorijević, Nikola
AU  - Radomirović, Mirjana Ž.
AU  - Rajković, Andreja
AU  - Nedić, Olgica
AU  - Ćirković-Veličković, Tanja
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6033
AB  - The French paradox describes a lower incidence of cardiovascular problems despite a high intake of saturated fats. This phenomenon was associated with higher consumption of red wine, only to be later discovered that the presence of several antioxidants, including resveratrol, are responsible for it. We investigated if resveratrol has a more direct role in protection from harmful oxidation and development of thrombosis, presumably through binding to important proteins of the blood coagulation process. Spectrofluorimetric analysis demonstrated binding of resveratrol to fibrinogen, the main protein in the coagulation process, which also has an important application as a food additive in making of fibrin gels. Various spectroscopic methods have demonstrated that binding of resveratrol does not unfold or destabilize fibrinogen since both near and far-UV CD spectra as well as its melting temperature remained unchanged. A mutually protective effect against the free radical-induced oxidation of resveratrol and fibrinogen was found. The presence of fibrinogen caused a very small masking effect of the antioxidative potential of resveratrol, measured by a reduction of hexacyanoferrate (III), while greatly increasing its solubility in an aqueous environment, thus increasing potential bioavailability and activity of resveratrol in circulation. By direct interaction and protection of fibrinogen, resveratrol may serve as an important antioxidant for prevention of thrombosis. The antioxidative effect of resveratrol may also protect and thus keep the desired characteristics of fibrinogen during the application of this protein as a food additive.
C3  - FoodEnTwin Symposium “Novel analytical approaches in food and environmental sciences”, Belgrade, Serbia, 16th-18th June, 2021
T1  - Resveratrol and fibrinogen interactions
SP  - 15
EP  - 15
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6033
ER  - 

@conference{
author = "Gligorijević, Nikola and Radomirović, Mirjana Ž. and Rajković, Andreja and Nedić, Olgica and Ćirković-Veličković, Tanja",
year = "2021",
abstract = "The French paradox describes a lower incidence of cardiovascular problems despite a high intake of saturated fats. This phenomenon was associated with higher consumption of red wine, only to be later discovered that the presence of several antioxidants, including resveratrol, are responsible for it. We investigated if resveratrol has a more direct role in protection from harmful oxidation and development of thrombosis, presumably through binding to important proteins of the blood coagulation process. Spectrofluorimetric analysis demonstrated binding of resveratrol to fibrinogen, the main protein in the coagulation process, which also has an important application as a food additive in making of fibrin gels. Various spectroscopic methods have demonstrated that binding of resveratrol does not unfold or destabilize fibrinogen since both near and far-UV CD spectra as well as its melting temperature remained unchanged. A mutually protective effect against the free radical-induced oxidation of resveratrol and fibrinogen was found. The presence of fibrinogen caused a very small masking effect of the antioxidative potential of resveratrol, measured by a reduction of hexacyanoferrate (III), while greatly increasing its solubility in an aqueous environment, thus increasing potential bioavailability and activity of resveratrol in circulation. By direct interaction and protection of fibrinogen, resveratrol may serve as an important antioxidant for prevention of thrombosis. The antioxidative effect of resveratrol may also protect and thus keep the desired characteristics of fibrinogen during the application of this protein as a food additive.",
journal = "FoodEnTwin Symposium “Novel analytical approaches in food and environmental sciences”, Belgrade, Serbia, 16th-18th June, 2021",
title = "Resveratrol and fibrinogen interactions",
pages = "15-15",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6033"
}

Gligorijević, N., Radomirović, M. Ž., Rajković, A., Nedić, O.,& Ćirković-Veličković, T.. (2021). Resveratrol and fibrinogen interactions. in FoodEnTwin Symposium “Novel analytical approaches in food and environmental sciences”, Belgrade, Serbia, 16th-18th June, 2021, 15-15.
https://hdl.handle.net/21.15107/rcub_cherry_6033

Gligorijević N, Radomirović MŽ, Rajković A, Nedić O, Ćirković-Veličković T. Resveratrol and fibrinogen interactions. in FoodEnTwin Symposium “Novel analytical approaches in food and environmental sciences”, Belgrade, Serbia, 16th-18th June, 2021. 2021;:15-15.
https://hdl.handle.net/21.15107/rcub_cherry_6033 .

Gligorijević, Nikola, Radomirović, Mirjana Ž., Rajković, Andreja, Nedić, Olgica, Ćirković-Veličković, Tanja, "Resveratrol and fibrinogen interactions" in FoodEnTwin Symposium “Novel analytical approaches in food and environmental sciences”, Belgrade, Serbia, 16th-18th June, 2021 (2021):15-15,
https://hdl.handle.net/21.15107/rcub_cherry_6033 .

TY  - CONF
AU  - Vasović, Tamara
AU  - Gligorijević, Nikola
AU  - Lević, Steva M.
AU  - Miljević, Čedo
AU  - Nedić, Olgica
AU  - Nikolić, Milan
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5134
AB  - Clozapine is an atypical antipsychotic used for the treatment of
schizophrenia. Prescribed daily doses of clozapine may reach
over 900 mg/day. Some studies reported a connection between
clozapine usage and the development of thrombosis. Our in vitro
study aimed to provide insight into molecular bases of this observation, investigating clozapine binding to isolated fibrinogen, the
main protein involved in hemostasis. Fibrinogen/clozapine interaction was confirmed by protein fluorescence quenching, with
affinity constant calculated to be 1.7 9 105 M1 and the number
of binding sites more than one. Direct interactions do not affect
the structure of fibrinogen, as determined by UV-VIS spectrometry and Fourier-transform infrared spectroscopy, nor fibrinogen
melting temperature, examined by fluorescence spectroscopy.
However, clozapine binding affected fibrin formation, by reducing coagulation speed and thickness of fibrin fibers. This behavior suggests that in the presence of clozapine, fibrinogen may
acquire thrombogenic characteristics. Although no difference in
fibrin gel porosity was detected, other factors present in the
blood may act synergistically with altered fibrin formation to
modify fibrin clot, thus increasing the risk for development of
thrombosis in individuals on clozapine treatment. By ORAC and
HORAC antioxidant assays, we found that clozapine efficiently
protects fibrinogen from free-radicals oxidation. Since the effect
of clozapine on fibrin formation is dose-dependent, it seems that
the dosage of the medication could be the main factor that determines if clozapine will have a more positive or negative effect on
fibrinogen and coagulation process in vivo.
PB  - FEBS Open Bio © 2021 FEBS
C3  - FEBS Open Bio, virtual 45th FEBS Congress from 3rd to 8th July 2021 (originally planned to be held in Ljubljana, Slovenia)
T1  - Atypical antipsychotic clozapine binds fibrinogen and affects fibrin formation
VL  - 11
SP  - 181
EP  - 181
DO  - doi:10.1002/2211-5463.13205
ER  - 

@conference{
author = "Vasović, Tamara and Gligorijević, Nikola and Lević, Steva M. and Miljević, Čedo and Nedić, Olgica and Nikolić, Milan",
year = "2021",
abstract = "Clozapine is an atypical antipsychotic used for the treatment of
schizophrenia. Prescribed daily doses of clozapine may reach
over 900 mg/day. Some studies reported a connection between
clozapine usage and the development of thrombosis. Our in vitro
study aimed to provide insight into molecular bases of this observation, investigating clozapine binding to isolated fibrinogen, the
main protein involved in hemostasis. Fibrinogen/clozapine interaction was confirmed by protein fluorescence quenching, with
affinity constant calculated to be 1.7 9 105 M1 and the number
of binding sites more than one. Direct interactions do not affect
the structure of fibrinogen, as determined by UV-VIS spectrometry and Fourier-transform infrared spectroscopy, nor fibrinogen
melting temperature, examined by fluorescence spectroscopy.
However, clozapine binding affected fibrin formation, by reducing coagulation speed and thickness of fibrin fibers. This behavior suggests that in the presence of clozapine, fibrinogen may
acquire thrombogenic characteristics. Although no difference in
fibrin gel porosity was detected, other factors present in the
blood may act synergistically with altered fibrin formation to
modify fibrin clot, thus increasing the risk for development of
thrombosis in individuals on clozapine treatment. By ORAC and
HORAC antioxidant assays, we found that clozapine efficiently
protects fibrinogen from free-radicals oxidation. Since the effect
of clozapine on fibrin formation is dose-dependent, it seems that
the dosage of the medication could be the main factor that determines if clozapine will have a more positive or negative effect on
fibrinogen and coagulation process in vivo.",
publisher = "FEBS Open Bio © 2021 FEBS",
journal = "FEBS Open Bio, virtual 45th FEBS Congress from 3rd to 8th July 2021 (originally planned to be held in Ljubljana, Slovenia)",
title = "Atypical antipsychotic clozapine binds fibrinogen and affects fibrin formation",
volume = "11",
pages = "181-181",
doi = "doi:10.1002/2211-5463.13205"
}

Vasović, T., Gligorijević, N., Lević, S. M., Miljević, Č., Nedić, O.,& Nikolić, M.. (2021). Atypical antipsychotic clozapine binds fibrinogen and affects fibrin formation. in FEBS Open Bio, virtual 45th FEBS Congress from 3rd to 8th July 2021 (originally planned to be held in Ljubljana, Slovenia)
FEBS Open Bio © 2021 FEBS., 11, 181-181.
https://doi.org/doi:10.1002/2211-5463.13205

Vasović T, Gligorijević N, Lević SM, Miljević Č, Nedić O, Nikolić M. Atypical antipsychotic clozapine binds fibrinogen and affects fibrin formation. in FEBS Open Bio, virtual 45th FEBS Congress from 3rd to 8th July 2021 (originally planned to be held in Ljubljana, Slovenia). 2021;11:181-181.
doi:doi:10.1002/2211-5463.13205 .

Vasović, Tamara, Gligorijević, Nikola, Lević, Steva M., Miljević, Čedo, Nedić, Olgica, Nikolić, Milan, "Atypical antipsychotic clozapine binds fibrinogen and affects fibrin formation" in FEBS Open Bio, virtual 45th FEBS Congress from 3rd to 8th July 2021 (originally planned to be held in Ljubljana, Slovenia), 11 (2021):181-181,
https://doi.org/doi:10.1002/2211-5463.13205 . .