TY  - JOUR
AU  - Bulatović, Sandra
AU  - Ilić, Mila V.
AU  - Šolević-Knudsen, Tatjana
AU  - Milić, Jelena
AU  - Pucarević, Mira
AU  - Jovančićević, Branimir
AU  - Vrvić, Miroslav M.
PY  - 2022
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4753
AB  - The oil pollutant in the Sava River aquifer in the residential area of Belgrade, Serbia was investigated in order to analyze the extent, origin and spatial distribution of the pollution, with the aim to estimate potential human health risks from exposure to the compounds detected. Analytical methods indicated that the dominant compounds in this oil pollutant were gasoline range organic compounds. Benzene, toluene, ethylbenzene and xylenes (BTEX) were identified as compounds of concern and quantified by headspace gas chromatography. The concentrations of benzene measured at all sampling points were higher than the remediation value while the maximum concentrations of BTEX quantified were among the highest concentrations of these compounds reported in the petroleum-contaminated aquifers in the world. The assessment of the human health risks from exposure to BTEX-covered industrial scenario for adult receptors and residential scenario for adult receptors and children. The exposure routes analyzed were dermal contact with and ingestion of contaminated water, considering both cancer and non-cancer effects. The analysis of the lifetime incremental cancer risk indicated the potential for adverse health effects for human exposure at the investigated location, and because of that it was interpreted as an unacceptable risk level or risks of high priority which required immediate consideration for remedial measures at this location. A complete set of mitigation measures was proposed including: groundwater decontamination treatment, installation of filters for tap water, development of the system for monitoring of BTEX in the groundwater and development of the emergency response capacities at this location.
PB  - Springer
T2  - Environmental Geochemistry and Health
T1  - Evaluation of potential human health risks from exposure to volatile organic compounds in contaminated urban groundwater in the Sava river aquifer, Belgrade, Serbia
VL  - 44
IS  - 10
SP  - 3451
EP  - 3472
DO  - 10.1007/s10653-021-01119-2
ER  - 

@article{
author = "Bulatović, Sandra and Ilić, Mila V. and Šolević-Knudsen, Tatjana and Milić, Jelena and Pucarević, Mira and Jovančićević, Branimir and Vrvić, Miroslav M.",
year = "2022",
abstract = "The oil pollutant in the Sava River aquifer in the residential area of Belgrade, Serbia was investigated in order to analyze the extent, origin and spatial distribution of the pollution, with the aim to estimate potential human health risks from exposure to the compounds detected. Analytical methods indicated that the dominant compounds in this oil pollutant were gasoline range organic compounds. Benzene, toluene, ethylbenzene and xylenes (BTEX) were identified as compounds of concern and quantified by headspace gas chromatography. The concentrations of benzene measured at all sampling points were higher than the remediation value while the maximum concentrations of BTEX quantified were among the highest concentrations of these compounds reported in the petroleum-contaminated aquifers in the world. The assessment of the human health risks from exposure to BTEX-covered industrial scenario for adult receptors and residential scenario for adult receptors and children. The exposure routes analyzed were dermal contact with and ingestion of contaminated water, considering both cancer and non-cancer effects. The analysis of the lifetime incremental cancer risk indicated the potential for adverse health effects for human exposure at the investigated location, and because of that it was interpreted as an unacceptable risk level or risks of high priority which required immediate consideration for remedial measures at this location. A complete set of mitigation measures was proposed including: groundwater decontamination treatment, installation of filters for tap water, development of the system for monitoring of BTEX in the groundwater and development of the emergency response capacities at this location.",
publisher = "Springer",
journal = "Environmental Geochemistry and Health",
title = "Evaluation of potential human health risks from exposure to volatile organic compounds in contaminated urban groundwater in the Sava river aquifer, Belgrade, Serbia",
volume = "44",
number = "10",
pages = "3451-3472",
doi = "10.1007/s10653-021-01119-2"
}

Bulatović, S., Ilić, M. V., Šolević-Knudsen, T., Milić, J., Pucarević, M., Jovančićević, B.,& Vrvić, M. M.. (2022). Evaluation of potential human health risks from exposure to volatile organic compounds in contaminated urban groundwater in the Sava river aquifer, Belgrade, Serbia. in Environmental Geochemistry and Health
Springer., 44(10), 3451-3472.
https://doi.org/10.1007/s10653-021-01119-2

Bulatović S, Ilić MV, Šolević-Knudsen T, Milić J, Pucarević M, Jovančićević B, Vrvić MM. Evaluation of potential human health risks from exposure to volatile organic compounds in contaminated urban groundwater in the Sava river aquifer, Belgrade, Serbia. in Environmental Geochemistry and Health. 2022;44(10):3451-3472.
doi:10.1007/s10653-021-01119-2 .

Bulatović, Sandra, Ilić, Mila V., Šolević-Knudsen, Tatjana, Milić, Jelena, Pucarević, Mira, Jovančićević, Branimir, Vrvić, Miroslav M., "Evaluation of potential human health risks from exposure to volatile organic compounds in contaminated urban groundwater in the Sava river aquifer, Belgrade, Serbia" in Environmental Geochemistry and Health, 44, no. 10 (2022):3451-3472,
https://doi.org/10.1007/s10653-021-01119-2 . .

TY  - JOUR
AU  - Beškoski, Vladimir
AU  - Gojgić-Cvijović, Gordana D.
AU  - Milic, Jelena S.
AU  - Ilić, Mila V.
AU  - Miletić, Srđan B.
AU  - Jovančićević, Branimir
AU  - Vrvić, Miroslav M.
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1317
AB  - The contamination of soil and water with petroleum and its products occurs due to accidental spills during exploitation, transport, processing, storing and use. In order to control the environmental risks caused by petroleum products a variety of techniques based on physical, chemical and biological methods have been used. Biological methods are considered to have a comparative advantage as cost effective and environmentally friendly technologies. Bioremediation, defined as the use of biological systems to destroy and reduce the concentrations of hazardous waste from contaminated sites, is an evolving technology for the removal and degradation of petroleum hydrocarbons as well as industrial solvents, phenols and pesticides. Microorganisms are the main bioremediation agents due to their diverse metabolic capacities. In order to enhance the rate of pollutant degradation the technology optimizes the conditions for the growth of microorganisms present in soil by aeration, nutrient addition and, if necessary, by adding separately prepared microbial cultures. The other factors that influence the efficiency of process are temperature, humidity, presence of surfactants, soil pH, mineral composition, content of organic substance of soil as well as type and concentration of contaminant. This paper presents a review of our ex situ bioremediation procedures successfully implemented on the industrial level. This technology was used for treatment of soils contaminated by crude oil and its derivatives originated from refinery as well as soils polluted with oil fuel and transformer oil.
AB  - Bioremedijacija je ekonomski isplativa, zelena tehnologija kojom se zagađujuće supstance biološkim putem transformišu u netoksična jedinjenja ili se potpuno razgrađuju do ugljendioksida i vode. Kao biološki agensi se najčešće koriste mikroorganizmi. Da bi se povećala brzina biološke razgradnje bioremedijacijom se optimizuju uslovi za rast mikroorganizama prisutnih u zemljištu aeracijom, dodavanjem hranljivih supstanci, najčešće soli azota i fosfora, a ako je potrebno i dodavanjem posebno pripremljenih kultura mikroorganizama. U ovom radu je dat i pregled naših ex situ bioremedijacionih postupaka na industrijskom nivou korišćenih za tretman zemljišta kontaminiranog naftnim ugljovodonicima kao što je zemljište iz rafinerije i zemljišta kontaminirana mazutom ili transformatorskim uljem.
PB  - Assoc Chemical Engineers Serbia, Belgrade
T2  - Hemijska industrija
T1  - Bioremediation of Soil Polluted with Crude Oil and Its Derivatives: Microorganisms, Degradation Pathways, Technologies
T1  - Bioremedijacija zemljišta kontaminiranog naftom i naftnim derivatima - mikroorganizmi, putanje razgradnje, tehnologije
VL  - 66
IS  - 2
SP  - 275
EP  - 289
DO  - 10.2298/HEMIND110824084B
ER  - 

@article{
author = "Beškoski, Vladimir and Gojgić-Cvijović, Gordana D. and Milic, Jelena S. and Ilić, Mila V. and Miletić, Srđan B. and Jovančićević, Branimir and Vrvić, Miroslav M.",
year = "2012",
abstract = "The contamination of soil and water with petroleum and its products occurs due to accidental spills during exploitation, transport, processing, storing and use. In order to control the environmental risks caused by petroleum products a variety of techniques based on physical, chemical and biological methods have been used. Biological methods are considered to have a comparative advantage as cost effective and environmentally friendly technologies. Bioremediation, defined as the use of biological systems to destroy and reduce the concentrations of hazardous waste from contaminated sites, is an evolving technology for the removal and degradation of petroleum hydrocarbons as well as industrial solvents, phenols and pesticides. Microorganisms are the main bioremediation agents due to their diverse metabolic capacities. In order to enhance the rate of pollutant degradation the technology optimizes the conditions for the growth of microorganisms present in soil by aeration, nutrient addition and, if necessary, by adding separately prepared microbial cultures. The other factors that influence the efficiency of process are temperature, humidity, presence of surfactants, soil pH, mineral composition, content of organic substance of soil as well as type and concentration of contaminant. This paper presents a review of our ex situ bioremediation procedures successfully implemented on the industrial level. This technology was used for treatment of soils contaminated by crude oil and its derivatives originated from refinery as well as soils polluted with oil fuel and transformer oil., Bioremedijacija je ekonomski isplativa, zelena tehnologija kojom se zagađujuće supstance biološkim putem transformišu u netoksična jedinjenja ili se potpuno razgrađuju do ugljendioksida i vode. Kao biološki agensi se najčešće koriste mikroorganizmi. Da bi se povećala brzina biološke razgradnje bioremedijacijom se optimizuju uslovi za rast mikroorganizama prisutnih u zemljištu aeracijom, dodavanjem hranljivih supstanci, najčešće soli azota i fosfora, a ako je potrebno i dodavanjem posebno pripremljenih kultura mikroorganizama. U ovom radu je dat i pregled naših ex situ bioremedijacionih postupaka na industrijskom nivou korišćenih za tretman zemljišta kontaminiranog naftnim ugljovodonicima kao što je zemljište iz rafinerije i zemljišta kontaminirana mazutom ili transformatorskim uljem.",
publisher = "Assoc Chemical Engineers Serbia, Belgrade",
journal = "Hemijska industrija",
title = "Bioremediation of Soil Polluted with Crude Oil and Its Derivatives: Microorganisms, Degradation Pathways, Technologies, Bioremedijacija zemljišta kontaminiranog naftom i naftnim derivatima - mikroorganizmi, putanje razgradnje, tehnologije",
volume = "66",
number = "2",
pages = "275-289",
doi = "10.2298/HEMIND110824084B"
}

Beškoski, V., Gojgić-Cvijović, G. D., Milic, J. S., Ilić, M. V., Miletić, S. B., Jovančićević, B.,& Vrvić, M. M.. (2012). Bioremediation of Soil Polluted with Crude Oil and Its Derivatives: Microorganisms, Degradation Pathways, Technologies. in Hemijska industrija
Assoc Chemical Engineers Serbia, Belgrade., 66(2), 275-289.
https://doi.org/10.2298/HEMIND110824084B

Beškoski V, Gojgić-Cvijović GD, Milic JS, Ilić MV, Miletić SB, Jovančićević B, Vrvić MM. Bioremediation of Soil Polluted with Crude Oil and Its Derivatives: Microorganisms, Degradation Pathways, Technologies. in Hemijska industrija. 2012;66(2):275-289.
doi:10.2298/HEMIND110824084B .

Beškoski, Vladimir, Gojgić-Cvijović, Gordana D., Milic, Jelena S., Ilić, Mila V., Miletić, Srđan B., Jovančićević, Branimir, Vrvić, Miroslav M., "Bioremediation of Soil Polluted with Crude Oil and Its Derivatives: Microorganisms, Degradation Pathways, Technologies" in Hemijska industrija, 66, no. 2 (2012):275-289,
https://doi.org/10.2298/HEMIND110824084B . .

TY  - JOUR
AU  - Milic, J. S.
AU  - Beškoski, Vladimir
AU  - Ranđelović, Danijela
AU  - Stojanovic, J.
AU  - Vrvić, Miroslav M.
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1541
AB  - This study visually documents the mechanical contact and interaction between the bacterial cells of two biogeocenotically different strains of Acidithiobacillus ferrooxidans (At. ferrooxidans) and oil shale containing pyrite. Atomic force microscopy (AFM) imaging was used to visualise initial interaction between the microorganisms and the surface minerals of an oil shale and to evaluate bacterial effects in the first hours of the bioleaching process. Acidithiobacillus ferrooxidans was attached to the shale surface already after 2 h, and after 48 h, numerous cells covered the surface with a biofilm. After 5 day incubation with At. ferrooxidans, AFM imaging revealed ellipsoid etched pits that represent footprints left by detached cells. Combining AFM surface imaging and leaching analysis following bacterial colonisation of oil shale layers demonstrates that an initial attachment to the surface is necessary for the leaching and that later on, once a sufficient concentration of Fe2+ ions in the solution is achieved, cells detach to become free cells, and leaching occurs primarily by the Fe3+. This experiment confirmed that microorganisms isolated from sites in which a particular substrate is found will demonstrate stronger binding to that substrate.
PB  - Technical Faculty, Bor-Serbia, Bor
T2  - Journal of mining and metallurgy, Section B: Metallurgy
T1  - Visualisation of the Interaction Between Acidithiobacillus Ferrooxidans and Oil Shale by Atomic Force Microscopy
VL  - 48
IS  - 2
SP  - 207
EP  - 217
DO  - 10.2298/JMMB110923016M
ER  - 

@article{
author = "Milic, J. S. and Beškoski, Vladimir and Ranđelović, Danijela and Stojanovic, J. and Vrvić, Miroslav M.",
year = "2012",
abstract = "This study visually documents the mechanical contact and interaction between the bacterial cells of two biogeocenotically different strains of Acidithiobacillus ferrooxidans (At. ferrooxidans) and oil shale containing pyrite. Atomic force microscopy (AFM) imaging was used to visualise initial interaction between the microorganisms and the surface minerals of an oil shale and to evaluate bacterial effects in the first hours of the bioleaching process. Acidithiobacillus ferrooxidans was attached to the shale surface already after 2 h, and after 48 h, numerous cells covered the surface with a biofilm. After 5 day incubation with At. ferrooxidans, AFM imaging revealed ellipsoid etched pits that represent footprints left by detached cells. Combining AFM surface imaging and leaching analysis following bacterial colonisation of oil shale layers demonstrates that an initial attachment to the surface is necessary for the leaching and that later on, once a sufficient concentration of Fe2+ ions in the solution is achieved, cells detach to become free cells, and leaching occurs primarily by the Fe3+. This experiment confirmed that microorganisms isolated from sites in which a particular substrate is found will demonstrate stronger binding to that substrate.",
publisher = "Technical Faculty, Bor-Serbia, Bor",
journal = "Journal of mining and metallurgy, Section B: Metallurgy",
title = "Visualisation of the Interaction Between Acidithiobacillus Ferrooxidans and Oil Shale by Atomic Force Microscopy",
volume = "48",
number = "2",
pages = "207-217",
doi = "10.2298/JMMB110923016M"
}

Milic, J. S., Beškoski, V., Ranđelović, D., Stojanovic, J.,& Vrvić, M. M.. (2012). Visualisation of the Interaction Between Acidithiobacillus Ferrooxidans and Oil Shale by Atomic Force Microscopy. in Journal of mining and metallurgy, Section B: Metallurgy
Technical Faculty, Bor-Serbia, Bor., 48(2), 207-217.
https://doi.org/10.2298/JMMB110923016M

Milic JS, Beškoski V, Ranđelović D, Stojanovic J, Vrvić MM. Visualisation of the Interaction Between Acidithiobacillus Ferrooxidans and Oil Shale by Atomic Force Microscopy. in Journal of mining and metallurgy, Section B: Metallurgy. 2012;48(2):207-217.
doi:10.2298/JMMB110923016M .

Milic, J. S., Beškoski, Vladimir, Ranđelović, Danijela, Stojanovic, J., Vrvić, Miroslav M., "Visualisation of the Interaction Between Acidithiobacillus Ferrooxidans and Oil Shale by Atomic Force Microscopy" in Journal of mining and metallurgy, Section B: Metallurgy, 48, no. 2 (2012):207-217,
https://doi.org/10.2298/JMMB110923016M . .

TY  - JOUR
AU  - Milić, Jelena
AU  - Gojgić-Cvijović, Gordana D.
AU  - Ilić, Mila V.
AU  - Šolević, Tatjana
AU  - Beškoski, Vladimir
AU  - Jovančićević, Branimir
AU  - Milović, Ana
AU  - Vrvić, Miroslav M.
PY  - 2007
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/81
AB  - Bioremediation is emerging as a promising technology for the treatment of soil and groundwater contamination. The technology is very effective in dealing with petroleum hydrocarbon contamination. However, bioremediation is a site-specific process and biodegradation by naturally occurring populations of microorganisms could be a major mechanism for the removal of petroleum from the environment. The procedure needs to be optimized in laboratory conditions and that’s why laboratory experiments are required before full-scale remediation can be successfully applied. The aim of this study was to examine the bioremediation potential of soil contaminated with crude oil. Two samples are examined using a biostimulation and bioventilation of zymogenous microbial consortia. TPH (total petroleum hydrocarbons) content in samples (1S and 2S) was: 103.2 and 412.6 g/kg of dry soil, respectively. We have also tested influence of one commercial surfactant on microbial population and TPH biodegradation. Experiments were performed in Erlenmeyer flasks (solid phase:mineral base = 1:9 m/V) on the shaker for 12 weeks. The microbial number is determined by dilution method on agar plates incubated on 26°C. Nutrient agar was used for total microbial number and mineral base medium (1 g/L NH4NO3, 0,25 g/L K2HPO4, 5 % soil extract, 1 % agar) with 2000 ppm diesel fuel for microbes which decompose hydrocarbons. The gas chromatography was used for determination of TPH content. During the test it has been achieved TPH biodegradation up to 86 % for 1S and 82 % for 2S, and number of hydrocarbon-decomposing microbes was significantly increased. Results from the test with surfactant showed even more TPH reduction. On basis of laboratory tests, biostimulation and bioventilation approaches could be successfully applied on industrial-scale bioremediation treatment.
AB  - Cilj ovog rada je bio da se ispita bioremedijacioni potencijal zemljišta kontaminiranog naftom. Ispitana su dva uzorka u uslovima biostimulacije i bioventilacije zimogenih konzorcijuma mikroorganizama. Sadržaj TPH (total petroleum hydrocarbons) u uzorcima (1S i 2S) bio je: 103,2, odnosno 412,6 g/kg suvog zemljišta. Takođe je ispitan i uticaj jednog komercijalnog surfaktanta na populaciju mikroorganizama i biodegradaciju TPH. Tokom trajanja testa postignuta je biodegradacija TPH od 86 % za 1S i 82 % za 2S, kao i značajno povećanje broja mikroorganizama, koji razgrađuju ugljovodonike. Rezultati testa sa surfaktantom su pokazali još veće smanjenje TPH. Na osnovu laboratorijskih testova, biostimulacija i bioventilacija se mogu uspešno primeniti za bioremedijacione postupke u industrijskim uslovima.
T2  - Zbornik radova Tehnološkog fakulteta, Leskovac
T1  - Bioremediation potential of contaminated soil
T1  - Bioremedijacioni potencijal kontaminiranog zemljišta
IS  - 18
SP  - 65
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_cherry_81
ER  - 

@article{
author = "Milić, Jelena and Gojgić-Cvijović, Gordana D. and Ilić, Mila V. and Šolević, Tatjana and Beškoski, Vladimir and Jovančićević, Branimir and Milović, Ana and Vrvić, Miroslav M.",
year = "2007",
abstract = "Bioremediation is emerging as a promising technology for the treatment of soil and groundwater contamination. The technology is very effective in dealing with petroleum hydrocarbon contamination. However, bioremediation is a site-specific process and biodegradation by naturally occurring populations of microorganisms could be a major mechanism for the removal of petroleum from the environment. The procedure needs to be optimized in laboratory conditions and that’s why laboratory experiments are required before full-scale remediation can be successfully applied. The aim of this study was to examine the bioremediation potential of soil contaminated with crude oil. Two samples are examined using a biostimulation and bioventilation of zymogenous microbial consortia. TPH (total petroleum hydrocarbons) content in samples (1S and 2S) was: 103.2 and 412.6 g/kg of dry soil, respectively. We have also tested influence of one commercial surfactant on microbial population and TPH biodegradation. Experiments were performed in Erlenmeyer flasks (solid phase:mineral base = 1:9 m/V) on the shaker for 12 weeks. The microbial number is determined by dilution method on agar plates incubated on 26°C. Nutrient agar was used for total microbial number and mineral base medium (1 g/L NH4NO3, 0,25 g/L K2HPO4, 5 % soil extract, 1 % agar) with 2000 ppm diesel fuel for microbes which decompose hydrocarbons. The gas chromatography was used for determination of TPH content. During the test it has been achieved TPH biodegradation up to 86 % for 1S and 82 % for 2S, and number of hydrocarbon-decomposing microbes was significantly increased. Results from the test with surfactant showed even more TPH reduction. On basis of laboratory tests, biostimulation and bioventilation approaches could be successfully applied on industrial-scale bioremediation treatment., Cilj ovog rada je bio da se ispita bioremedijacioni potencijal zemljišta kontaminiranog naftom. Ispitana su dva uzorka u uslovima biostimulacije i bioventilacije zimogenih konzorcijuma mikroorganizama. Sadržaj TPH (total petroleum hydrocarbons) u uzorcima (1S i 2S) bio je: 103,2, odnosno 412,6 g/kg suvog zemljišta. Takođe je ispitan i uticaj jednog komercijalnog surfaktanta na populaciju mikroorganizama i biodegradaciju TPH. Tokom trajanja testa postignuta je biodegradacija TPH od 86 % za 1S i 82 % za 2S, kao i značajno povećanje broja mikroorganizama, koji razgrađuju ugljovodonike. Rezultati testa sa surfaktantom su pokazali još veće smanjenje TPH. Na osnovu laboratorijskih testova, biostimulacija i bioventilacija se mogu uspešno primeniti za bioremedijacione postupke u industrijskim uslovima.",
journal = "Zbornik radova Tehnološkog fakulteta, Leskovac",
title = "Bioremediation potential of contaminated soil, Bioremedijacioni potencijal kontaminiranog zemljišta",
number = "18",
pages = "65-69",
url = "https://hdl.handle.net/21.15107/rcub_cherry_81"
}

Milić, J., Gojgić-Cvijović, G. D., Ilić, M. V., Šolević, T., Beškoski, V., Jovančićević, B., Milović, A.,& Vrvić, M. M.. (2007). Bioremediation potential of contaminated soil. in Zbornik radova Tehnološkog fakulteta, Leskovac(18), 65-69.
https://hdl.handle.net/21.15107/rcub_cherry_81

Milić J, Gojgić-Cvijović GD, Ilić MV, Šolević T, Beškoski V, Jovančićević B, Milović A, Vrvić MM. Bioremediation potential of contaminated soil. in Zbornik radova Tehnološkog fakulteta, Leskovac. 2007;(18):65-69.
https://hdl.handle.net/21.15107/rcub_cherry_81 .

Milić, Jelena, Gojgić-Cvijović, Gordana D., Ilić, Mila V., Šolević, Tatjana, Beškoski, Vladimir, Jovančićević, Branimir, Milović, Ana, Vrvić, Miroslav M., "Bioremediation potential of contaminated soil" in Zbornik radova Tehnološkog fakulteta, Leskovac, no. 18 (2007):65-69,
https://hdl.handle.net/21.15107/rcub_cherry_81 .