TY  - CONF
AU  - Minić, Simeon L.
AU  - Jovanović, Zorana
AU  - Veličlović, Luka
AU  - Gligorijević, Nikola
AU  - Zoumpanioti, Maria
AU  - Nikolić, Milan
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5786
AB  - Stabilization of the vivid colors of phycobiliproteins of micro/macroalgae is a prerequisite
for their greater use in the food industry. Phycocyanin (C-PC) was purified from
cyanobacteria Spirulina (Arthrospira) Pacifica raw extract by ammonium sulfate protein
precipitation and anion ion-exchange chromatography. Purity was confirmed
electrophoretically (SDS-PAGE). The binding of ten selected bioactive polyphenols to CPC (including quercetin, coenzyme Q10, gallic acid, vanillic acid, and resveratrol) was
examined by standard spectroscopic methods. Quercetin is shown to have the strongest
binding (Ka~3x105
 M-1), with stabilization of the secondary protein structure under
physiological conditions.
AB  - Stabilizacija živopisnih boja fikobilinskih proteina mikro/makroalgi preduslov je za
njihovo veće korišćenje u industriji hrane. Fikocijanin (C-PC) je prečišćen iz sirovog
ekstrakta cijanobakterije Spirulina (Arthrospira) Pacifica, taloženjem proteina amonijumsulfatom i anjonskom jonoizmenjivačkom hromatografijom. Čistoća je potvrđena
elektroforetski (SDS-PAGE). Vezivanje deset odabranih bioaktivnih polifenola za C-PC
(uključujući kvarcetin, koenzim Q10, galnu kiselinu, vanilinsku kiselinu i resveratrol)
ispitano je standardnim spektroskopskim metodama. Kvarcetin je pokazao najjače
vezivanje (Ka~3x105
 M-1), uz stabilizaciju sekundarne strukture proteina pod fiziološkim
uslovima.
PB  - Belgrade : Serbian Chemical Society
C3  - 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, 9th-10th June, 2022. In: Book of Abstracts and Proceedings
T1  - Phycocyanin from microalgae Spirulina: purification and binding of selected (poly)phenols
SP  - 62
EP  - 62
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5786
ER  - 

@conference{
author = "Minić, Simeon L. and Jovanović, Zorana and Veličlović, Luka and Gligorijević, Nikola and Zoumpanioti, Maria and Nikolić, Milan",
year = "2022",
abstract = "Stabilization of the vivid colors of phycobiliproteins of micro/macroalgae is a prerequisite
for their greater use in the food industry. Phycocyanin (C-PC) was purified from
cyanobacteria Spirulina (Arthrospira) Pacifica raw extract by ammonium sulfate protein
precipitation and anion ion-exchange chromatography. Purity was confirmed
electrophoretically (SDS-PAGE). The binding of ten selected bioactive polyphenols to CPC (including quercetin, coenzyme Q10, gallic acid, vanillic acid, and resveratrol) was
examined by standard spectroscopic methods. Quercetin is shown to have the strongest
binding (Ka~3x105
 M-1), with stabilization of the secondary protein structure under
physiological conditions., Stabilizacija živopisnih boja fikobilinskih proteina mikro/makroalgi preduslov je za
njihovo veće korišćenje u industriji hrane. Fikocijanin (C-PC) je prečišćen iz sirovog
ekstrakta cijanobakterije Spirulina (Arthrospira) Pacifica, taloženjem proteina amonijumsulfatom i anjonskom jonoizmenjivačkom hromatografijom. Čistoća je potvrđena
elektroforetski (SDS-PAGE). Vezivanje deset odabranih bioaktivnih polifenola za C-PC
(uključujući kvarcetin, koenzim Q10, galnu kiselinu, vanilinsku kiselinu i resveratrol)
ispitano je standardnim spektroskopskim metodama. Kvarcetin je pokazao najjače
vezivanje (Ka~3x105
 M-1), uz stabilizaciju sekundarne strukture proteina pod fiziološkim
uslovima.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, 9th-10th June, 2022. In: Book of Abstracts and Proceedings",
title = "Phycocyanin from microalgae Spirulina: purification and binding of selected (poly)phenols",
pages = "62-62",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5786"
}

Minić, S. L., Jovanović, Z., Veličlović, L., Gligorijević, N., Zoumpanioti, M.,& Nikolić, M.. (2022). Phycocyanin from microalgae Spirulina: purification and binding of selected (poly)phenols. in 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, 9th-10th June, 2022. In: Book of Abstracts and Proceedings
Belgrade : Serbian Chemical Society., 62-62.
https://hdl.handle.net/21.15107/rcub_cherry_5786

Minić SL, Jovanović Z, Veličlović L, Gligorijević N, Zoumpanioti M, Nikolić M. Phycocyanin from microalgae Spirulina: purification and binding of selected (poly)phenols. in 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, 9th-10th June, 2022. In: Book of Abstracts and Proceedings. 2022;:62-62.
https://hdl.handle.net/21.15107/rcub_cherry_5786 .

Minić, Simeon L., Jovanović, Zorana, Veličlović, Luka, Gligorijević, Nikola, Zoumpanioti, Maria, Nikolić, Milan, "Phycocyanin from microalgae Spirulina: purification and binding of selected (poly)phenols" in 58th Meeting of the Serbian Chemical Society, Belgrade, Serbia, 9th-10th June, 2022. In: Book of Abstracts and Proceedings (2022):62-62,
https://hdl.handle.net/21.15107/rcub_cherry_5786 .