Ćirković-Veličković, Tanja

Link to this page

Authority KeyName Variants
orcid::0000-0003-2559-5234
  • Ćirković-Veličković, Tanja (355)
  • Ćirković Veličković, Tanja (4)
Projects
Molecular properties and modifications of some respiratory and nutritional allergens FoodEnTwin-Twinning of research activities for the frontier research in the fields of food, nutrition and environmental omics
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200168 (University of Belgrade, Faculty of Chemistry) Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research
Imptox (An innovative analytical platform to investigate the effect and toxicity of micro and nano plastics combined with environmental contaminants on the risk of allergic disease in preclinical and clinical) Ispitivanje strukture i funkcije biološki važnih makromolekula u fiziološkim i patološkim stanjima
Ghent University Global Campus, Belgian Special Research Fund BOF StG No. 01N01718. CAPSIDO – Developement of the assays for detection of SARS Cov-2 virus capsid proteins in biological fluids of COVID19 patients
Serbian Academy of Sciences and Arts Project F-26. Serbian Academy of Sciences and Arts GA No. F-26.
Swedish Research Council King Gustaf V 80th Birthday Foundation
Magnus Bergvall Foundation Swedish Asthma and Allergy Associations Research Foundation
Swedish Cancer and Allergy Foundation Swedish Heart-Lung Foundation
Rational design and synthesis of biologically active and coordination compounds and functional materials, relevant for (bio)nanotechnology Stockholm County Council
Hesselman Foundation Structure-properties relationships of natural and synthetic molecules and their metal complexes
Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance Karolinska Institutet
COST Action [FA 1005] ShellPCR - Development of Elisa and Immuno-PCR for Sensitive and Specific Detection of Shellfish Tropomyosin
Serbian Academy of Sciences and Arts, grant number F-26 Swedish Association for Allergology
Belgian Special Research Fund BOF StG No. 01N01718 Belgian Special Research Fund BOF StG No. 01N01718.
Center for Inflammatory Diseases Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring

Author's Bibliography

Insights into oxidative protein modifications in shellfish allergens: Impact of GI digestion following thermal treatment

Đukić, Teodora; Khulal, Urmila; Smiljanić, Katarina; Vasović, Tamara; Aćimović, Jelena; Rajković, Andreja; Ćirković Veličković, Tanja

(University of Belgrade – Faculty of Chemistry, 2024)

TY  - CONF
AU  - Đukić, Teodora
AU  - Khulal, Urmila
AU  - Smiljanić, Katarina
AU  - Vasović, Tamara
AU  - Aćimović, Jelena
AU  - Rajković, Andreja
AU  - Ćirković Veličković, Tanja
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6539
AB  - Protein modifications (PMs) are covalent changes occurring on amino acid (AA) side chains of
proteins via enzymatic action or spontaneously. They increase the protein structure complexity
from the level of the genome to the proteome, and can determine their activity and, interactions1.
It has been shown, on myofibrillar fish proteins that oxidative modifications depending on their
saturation can decrease or enhance protein digestability2. Thus in this study shellfish meat samples
were subjected to cooking followed by simulated INFOGEST in vitro gastrointestinal digestion
protocol. Mass Spectrometry and in gel based proteomics, were used to detect PMs and digestion
resistan peptides of raw and cooked shellfish meat samples with a foucs on major shellfish allergens:
sarcoplasmic calcium-binding protein (SBP) and tropomyosin (TPM). Lastly, identified PMs
were marked on TPM alignment from shrimp, oyster and abalone species, to gain insight into their
pattern on the known IgE binding epitopes. Focusing on oxidative PMs, mostly on oxidation of Met,
it was confirmed that, in all shellfish samples, PMs on TPM and SBP were more predominant after
cooking. For instance in abalone, double oxidation of Met was only detected in cooked samples
(sulfone M68 and M81). Another indicator of oxidative stress, 4-hydroxynonenal (HNE), was identified
on shrimp TPM and, notably HNE was 21 times more abundant in the cooked sample. Study
highlights the susceptibility of immunodominant epitopes in major shellfish allergens to oxidative
PMs, which could impact interactions with the immune system in sensitive individuals. Significant
resistance to digestion was demonstrated by paramyosin fragments in abalone and oyster suggesting
the need for further analysis of their allergenicity. SBP of raw shrimp was strikingly resistant
to both gastric and intestinal digestion. Moreover, our results indicate that oxidative modifications
may decrease the stability of proteins like shrimp TPM, making them more digestible in thermally
treated samples, highlighting a potential strategy for reducing allergenicity.
PB  - University of Belgrade – Faculty of Chemistry
C3  - VII Symposium of the Serbian Proteomics Association – SePA “Application of proteomics in biomedicine”, Belgrade, Serbia June 6th, 2024
T1  - Insights into oxidative protein modifications in shellfish allergens: Impact of GI digestion following thermal treatment
SP  - 10
EP  - 10
ER  - 
@conference{
author = "Đukić, Teodora and Khulal, Urmila and Smiljanić, Katarina and Vasović, Tamara and Aćimović, Jelena and Rajković, Andreja and Ćirković Veličković, Tanja",
year = "2024",
abstract = "Protein modifications (PMs) are covalent changes occurring on amino acid (AA) side chains of
proteins via enzymatic action or spontaneously. They increase the protein structure complexity
from the level of the genome to the proteome, and can determine their activity and, interactions1.
It has been shown, on myofibrillar fish proteins that oxidative modifications depending on their
saturation can decrease or enhance protein digestability2. Thus in this study shellfish meat samples
were subjected to cooking followed by simulated INFOGEST in vitro gastrointestinal digestion
protocol. Mass Spectrometry and in gel based proteomics, were used to detect PMs and digestion
resistan peptides of raw and cooked shellfish meat samples with a foucs on major shellfish allergens:
sarcoplasmic calcium-binding protein (SBP) and tropomyosin (TPM). Lastly, identified PMs
were marked on TPM alignment from shrimp, oyster and abalone species, to gain insight into their
pattern on the known IgE binding epitopes. Focusing on oxidative PMs, mostly on oxidation of Met,
it was confirmed that, in all shellfish samples, PMs on TPM and SBP were more predominant after
cooking. For instance in abalone, double oxidation of Met was only detected in cooked samples
(sulfone M68 and M81). Another indicator of oxidative stress, 4-hydroxynonenal (HNE), was identified
on shrimp TPM and, notably HNE was 21 times more abundant in the cooked sample. Study
highlights the susceptibility of immunodominant epitopes in major shellfish allergens to oxidative
PMs, which could impact interactions with the immune system in sensitive individuals. Significant
resistance to digestion was demonstrated by paramyosin fragments in abalone and oyster suggesting
the need for further analysis of their allergenicity. SBP of raw shrimp was strikingly resistant
to both gastric and intestinal digestion. Moreover, our results indicate that oxidative modifications
may decrease the stability of proteins like shrimp TPM, making them more digestible in thermally
treated samples, highlighting a potential strategy for reducing allergenicity.",
publisher = "University of Belgrade – Faculty of Chemistry",
journal = "VII Symposium of the Serbian Proteomics Association – SePA “Application of proteomics in biomedicine”, Belgrade, Serbia June 6th, 2024",
title = "Insights into oxidative protein modifications in shellfish allergens: Impact of GI digestion following thermal treatment",
pages = "10-10"
}
Đukić, T., Khulal, U., Smiljanić, K., Vasović, T., Aćimović, J., Rajković, A.,& Ćirković Veličković, T.. (2024). Insights into oxidative protein modifications in shellfish allergens: Impact of GI digestion following thermal treatment. in VII Symposium of the Serbian Proteomics Association – SePA “Application of proteomics in biomedicine”, Belgrade, Serbia June 6th, 2024
University of Belgrade – Faculty of Chemistry., 10-10.
Đukić T, Khulal U, Smiljanić K, Vasović T, Aćimović J, Rajković A, Ćirković Veličković T. Insights into oxidative protein modifications in shellfish allergens: Impact of GI digestion following thermal treatment. in VII Symposium of the Serbian Proteomics Association – SePA “Application of proteomics in biomedicine”, Belgrade, Serbia June 6th, 2024. 2024;:10-10..
Đukić, Teodora, Khulal, Urmila, Smiljanić, Katarina, Vasović, Tamara, Aćimović, Jelena, Rajković, Andreja, Ćirković Veličković, Tanja, "Insights into oxidative protein modifications in shellfish allergens: Impact of GI digestion following thermal treatment" in VII Symposium of the Serbian Proteomics Association – SePA “Application of proteomics in biomedicine”, Belgrade, Serbia June 6th, 2024 (2024):10-10.

Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties

Gligorijević, Nikola; Lujić, Tamara; Mutić, Tamara; Vasović, Tamara; Aćimović, Jelena; de Guzman, Maria Krishna; Stanić-Vučinić, Dragana; Ćirković-Veličković, Tanja

(Elsevier, 2024)

TY  - JOUR
AU  - Gligorijević, Nikola
AU  - Lujić, Tamara
AU  - Mutić, Tamara
AU  - Vasović, Tamara
AU  - Aćimović, Jelena
AU  - de Guzman, Maria Krishna
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6510
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T1  - Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties
VL  - 267
SP  - 131564
DO  - 10.1016/j.ijbiomac.2024.131564
ER  - 
@article{
author = "Gligorijević, Nikola and Lujić, Tamara and Mutić, Tamara and Vasović, Tamara and Aćimović, Jelena and de Guzman, Maria Krishna and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2024",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules",
title = "Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties",
volume = "267",
pages = "131564",
doi = "10.1016/j.ijbiomac.2024.131564"
}
Gligorijević, N., Lujić, T., Mutić, T., Vasović, T., Aćimović, J., de Guzman, M. K., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2024). Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties. in International Journal of Biological Macromolecules
Elsevier., 267, 131564.
https://doi.org/10.1016/j.ijbiomac.2024.131564
Gligorijević N, Lujić T, Mutić T, Vasović T, Aćimović J, de Guzman MK, Stanić-Vučinić D, Ćirković-Veličković T. Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties. in International Journal of Biological Macromolecules. 2024;267:131564.
doi:10.1016/j.ijbiomac.2024.131564 .
Gligorijević, Nikola, Lujić, Tamara, Mutić, Tamara, Vasović, Tamara, Aćimović, Jelena, de Guzman, Maria Krishna, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties" in International Journal of Biological Macromolecules, 267 (2024):131564,
https://doi.org/10.1016/j.ijbiomac.2024.131564 . .

Research data no. 1 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets

Mutić, Tamara; Stanić-Vučinić, Dragana; Mutić, Jelena; Ilić, Miloš; Jovanović, Vesna; Aćimović, Jelena; Anđelković, Boban; Krishna de Guzman, Maria; Anđelković, Mirjana; Turkalj, Mirjana; Ćirković-Veličković, Tanja

(2024)

TY  - DATA
AU  - Mutić, Tamara
AU  - Stanić-Vučinić, Dragana
AU  - Mutić, Jelena
AU  - Ilić, Miloš
AU  - Jovanović, Vesna
AU  - Aćimović, Jelena
AU  - Anđelković, Boban
AU  - Krishna de Guzman, Maria
AU  - Anđelković, Mirjana
AU  - Turkalj, Mirjana
AU  - Ćirković-Veličković, Tanja
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6484
AB  - The experimental data for the results shown in the manuscript "Microplastic contamination of edible parts of commercially important mussels, clams and shrimps in different markets". Statistical investigation of the presence of microplastics in bivalve species using microFTIR. This dataset includes samples from various origins, including Sebia, South Korea, Croatia and Belgium, and includes species as diverse as shrimp, mussels and clams.
T1  - Research data no. 1 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6484
ER  - 
@misc{
author = "Mutić, Tamara and Stanić-Vučinić, Dragana and Mutić, Jelena and Ilić, Miloš and Jovanović, Vesna and Aćimović, Jelena and Anđelković, Boban and Krishna de Guzman, Maria and Anđelković, Mirjana and Turkalj, Mirjana and Ćirković-Veličković, Tanja",
year = "2024",
abstract = "The experimental data for the results shown in the manuscript "Microplastic contamination of edible parts of commercially important mussels, clams and shrimps in different markets". Statistical investigation of the presence of microplastics in bivalve species using microFTIR. This dataset includes samples from various origins, including Sebia, South Korea, Croatia and Belgium, and includes species as diverse as shrimp, mussels and clams.",
title = "Research data no. 1 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6484"
}
Mutić, T., Stanić-Vučinić, D., Mutić, J., Ilić, M., Jovanović, V., Aćimović, J., Anđelković, B., Krishna de Guzman, M., Anđelković, M., Turkalj, M.,& Ćirković-Veličković, T.. (2024). Research data no. 1 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets. .
https://hdl.handle.net/21.15107/rcub_cherry_6484
Mutić T, Stanić-Vučinić D, Mutić J, Ilić M, Jovanović V, Aćimović J, Anđelković B, Krishna de Guzman M, Anđelković M, Turkalj M, Ćirković-Veličković T. Research data no. 1 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets. 2024;.
https://hdl.handle.net/21.15107/rcub_cherry_6484 .
Mutić, Tamara, Stanić-Vučinić, Dragana, Mutić, Jelena, Ilić, Miloš, Jovanović, Vesna, Aćimović, Jelena, Anđelković, Boban, Krishna de Guzman, Maria, Anđelković, Mirjana, Turkalj, Mirjana, Ćirković-Veličković, Tanja, "Research data no. 1 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets" (2024),
https://hdl.handle.net/21.15107/rcub_cherry_6484 .

Research data no. 2 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties

Gligorijević, Nikola; Lujić, Tamara; Mutić, Tamara; Vasović, Tamara; Aćimović, Jelena; de Guzman, Maria Krishna; Stanić-Vučinić, Dragana; Ćirković-Veličković, Tanja

(2024)

TY  - DATA
AU  - Gligorijević, Nikola
AU  - Lujić, Tamara
AU  - Mutić, Tamara
AU  - Vasović, Tamara
AU  - Aćimović, Jelena
AU  - de Guzman, Maria Krishna
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6469
AB  - Research data for the unpublished paper: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties. Densitometry analysis of ovalbumin band intensity after digestion in the presence of microplastics on SDS-PAGE gels.
T1  - Research data no. 2 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6469
ER  - 
@misc{
author = "Gligorijević, Nikola and Lujić, Tamara and Mutić, Tamara and Vasović, Tamara and Aćimović, Jelena and de Guzman, Maria Krishna and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2024",
abstract = "Research data for the unpublished paper: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties. Densitometry analysis of ovalbumin band intensity after digestion in the presence of microplastics on SDS-PAGE gels.",
title = "Research data no. 2 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6469"
}
Gligorijević, N., Lujić, T., Mutić, T., Vasović, T., Aćimović, J., de Guzman, M. K., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2024). Research data no. 2 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties. .
https://hdl.handle.net/21.15107/rcub_cherry_6469
Gligorijević N, Lujić T, Mutić T, Vasović T, Aćimović J, de Guzman MK, Stanić-Vučinić D, Ćirković-Veličković T. Research data no. 2 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties. 2024;.
https://hdl.handle.net/21.15107/rcub_cherry_6469 .
Gligorijević, Nikola, Lujić, Tamara, Mutić, Tamara, Vasović, Tamara, Aćimović, Jelena, de Guzman, Maria Krishna, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Research data no. 2 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties" (2024),
https://hdl.handle.net/21.15107/rcub_cherry_6469 .

Research data no. 1 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties

Gligorijević, Nikola; Lujić, Tamara; Mutić, Tamara; Vasović, Tamara; de Guzman, Maria Krishna; Aćimović, Jelena; Stanić-Vučinić, Dragana; Ćirković-Veličković, Tanja

(2024)

TY  - DATA
AU  - Gligorijević, Nikola
AU  - Lujić, Tamara
AU  - Mutić, Tamara
AU  - Vasović, Tamara
AU  - de Guzman, Maria Krishna
AU  - Aćimović, Jelena
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6465
AB  - Data used for the analysis of pepsin interaction with polystyrene microplastic of 10 and 100 µm in size. Binding isotherms data were used for the construction of binding isotherms from which binding between pepsin and polystyrene microplastic was described. Structural features of pepsin in the presence of polystyrene were analysed by CD spectrometry and spectrofluorimetry.
T1  - Research data no. 1 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6465
ER  - 
@misc{
author = "Gligorijević, Nikola and Lujić, Tamara and Mutić, Tamara and Vasović, Tamara and de Guzman, Maria Krishna and Aćimović, Jelena and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2024",
abstract = "Data used for the analysis of pepsin interaction with polystyrene microplastic of 10 and 100 µm in size. Binding isotherms data were used for the construction of binding isotherms from which binding between pepsin and polystyrene microplastic was described. Structural features of pepsin in the presence of polystyrene were analysed by CD spectrometry and spectrofluorimetry.",
title = "Research data no. 1 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6465"
}
Gligorijević, N., Lujić, T., Mutić, T., Vasović, T., de Guzman, M. K., Aćimović, J., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2024). Research data no. 1 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties. .
https://hdl.handle.net/21.15107/rcub_cherry_6465
Gligorijević N, Lujić T, Mutić T, Vasović T, de Guzman MK, Aćimović J, Stanić-Vučinić D, Ćirković-Veličković T. Research data no. 1 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties. 2024;.
https://hdl.handle.net/21.15107/rcub_cherry_6465 .
Gligorijević, Nikola, Lujić, Tamara, Mutić, Tamara, Vasović, Tamara, de Guzman, Maria Krishna, Aćimović, Jelena, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Research data no. 1 for the manuscript: Ovalbumin interaction with polystyrene and polyethylene terephthalate microplastics alters its structural properties" (2024),
https://hdl.handle.net/21.15107/rcub_cherry_6465 .

Protein modifications screening of raw and thermally treated meat gastrointestinal digesta

Khulal, Urmila; Đukić, Teodora; Smiljanić, Katarina; Vasović, Tamara; Aćimović, Jelena; Rajković, Andreja; Ćirković-Veličković, Tanja

(Elsevier, 2024)

TY  - JOUR
AU  - Khulal, Urmila
AU  - Đukić, Teodora
AU  - Smiljanić, Katarina
AU  - Vasović, Tamara
AU  - Aćimović, Jelena
AU  - Rajković, Andreja
AU  - Ćirković-Veličković, Tanja
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6437
AB  - Meat samples were subjected to thermal processing combined with simulated INFOGEST in vitro gastrointestinal (GI) digestion. Protein modifications (PMs) were screened with commercially available PM-specific antibodies. Specific proteins at 20, 37, 50, and 65 kDa react to more than 3 PM-specific antibodies among meat proteins. Lysine methylation and methionine oxidation were the most prominent PMs in WB. Mass spectrometry confirmed bands at ≈20 kDa as allergenic proteins: sarcoplasmic calcium-binding protein in oyster, 37 kDa as tropomyosin in shrimp, oyster, and abalone. MS-identified PMs of shellfish allergens were aligned to the IgE binding epitopes. GI digestion-resistant peptides of shellfish proteins were identified as paramyosins in oyster and abalone and SBP in shrimp. Our results point to the high susceptibility of immunodominant epitopes of major shellfish allergens to PMs. In TPM, saturation of oxidative modification increases with thermal processing resulting in higher susceptibility of TPM to gastric digestion.
PB  - Elsevier
T2  - Journal of Functional Foods
T1  - Protein modifications screening of raw and thermally treated meat gastrointestinal digesta
VL  - 113
SP  - 106052
DO  - 10.1016/j.jff.2024.106052
ER  - 
@article{
author = "Khulal, Urmila and Đukić, Teodora and Smiljanić, Katarina and Vasović, Tamara and Aćimović, Jelena and Rajković, Andreja and Ćirković-Veličković, Tanja",
year = "2024",
abstract = "Meat samples were subjected to thermal processing combined with simulated INFOGEST in vitro gastrointestinal (GI) digestion. Protein modifications (PMs) were screened with commercially available PM-specific antibodies. Specific proteins at 20, 37, 50, and 65 kDa react to more than 3 PM-specific antibodies among meat proteins. Lysine methylation and methionine oxidation were the most prominent PMs in WB. Mass spectrometry confirmed bands at ≈20 kDa as allergenic proteins: sarcoplasmic calcium-binding protein in oyster, 37 kDa as tropomyosin in shrimp, oyster, and abalone. MS-identified PMs of shellfish allergens were aligned to the IgE binding epitopes. GI digestion-resistant peptides of shellfish proteins were identified as paramyosins in oyster and abalone and SBP in shrimp. Our results point to the high susceptibility of immunodominant epitopes of major shellfish allergens to PMs. In TPM, saturation of oxidative modification increases with thermal processing resulting in higher susceptibility of TPM to gastric digestion.",
publisher = "Elsevier",
journal = "Journal of Functional Foods",
title = "Protein modifications screening of raw and thermally treated meat gastrointestinal digesta",
volume = "113",
pages = "106052",
doi = "10.1016/j.jff.2024.106052"
}
Khulal, U., Đukić, T., Smiljanić, K., Vasović, T., Aćimović, J., Rajković, A.,& Ćirković-Veličković, T.. (2024). Protein modifications screening of raw and thermally treated meat gastrointestinal digesta. in Journal of Functional Foods
Elsevier., 113, 106052.
https://doi.org/10.1016/j.jff.2024.106052
Khulal U, Đukić T, Smiljanić K, Vasović T, Aćimović J, Rajković A, Ćirković-Veličković T. Protein modifications screening of raw and thermally treated meat gastrointestinal digesta. in Journal of Functional Foods. 2024;113:106052.
doi:10.1016/j.jff.2024.106052 .
Khulal, Urmila, Đukić, Teodora, Smiljanić, Katarina, Vasović, Tamara, Aćimović, Jelena, Rajković, Andreja, Ćirković-Veličković, Tanja, "Protein modifications screening of raw and thermally treated meat gastrointestinal digesta" in Journal of Functional Foods, 113 (2024):106052,
https://doi.org/10.1016/j.jff.2024.106052 . .

Mass spectrometry analysis reveals impact of peanut roasting on post-translational modifications of key allergens and their hinderance of trypsin digestion

Đukić, Teodora; Smiljanić, Katarina; Prodić, Ivana; Ćirković Veličković, Tanja

(Faculty of Chemistry, Serbian Chemical Society, 2023)

TY  - CONF
AU  - Đukić, Teodora
AU  - Smiljanić, Katarina
AU  - Prodić, Ivana
AU  - Ćirković Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6538
AB  - A bottom-up proteomic study, using high-resolution tandem mass spectrometry (HRMS) and, PEAKS Studio X+ was performed to investigate the impact of peanut roasting on readily soluble allergens and their post-translational modification (PTM) profiles. Among four major peanut allergen groups, we found that Ara h 3 prevails in raw peanut extract (PE), similar to Ara h 1, while the opposite is true for Ara h 6, which is enriched in roasted PE; Ara h 2 bands are near the same intensity. HRMS detected more than 40 different types of modification in raw and roasted samples. Distinct variations in the types and occurrence of specific amino acid PTMs were identified between allergens present in raw and roasted samples. Roasting affected the most frequent modifications by enrichment of Ox, HyP, carbamoylation (KR), and deamidation. The PMs could also be mapped to the regions of IgE-binding epitopes of Ara h 1-3 and Ara h 6. As porcine trypsin is used for HRMS sample preparation and is also a digestive protease, hindrance effects to trypsin efficacy regarding PTMs were assessed. Roasting caused dihydroxylation and formylation PTMs with hindrance effects to trypsin efficacy, while methylation on several K/R showed opposite effects. In the case of methylated R342, results suggested facilitation of tryptic performance at modified residue compared to unmodified counterpart, while in the rest of the seven genuine sequences containing modified residues, the trend was opposite. Further exploration of how different PTMs could affect digestion efficiencies of major gastric and intestinal peptidases is currently in works and a much-needed assessment to better understand the role of PTMs.
PB  - Faculty of Chemistry, Serbian Chemical Society
C3  - Book of Abstracts, Serbian Biochemical Society Twelfth Conference "Bicehemistry in Biotechnology", September 21st, 2023
T1  - Mass spectrometry analysis reveals impact of peanut roasting on post-translational modifications of key allergens and  their hinderance of trypsin digestion
SP  - 146
EP  - 146
ER  - 
@conference{
author = "Đukić, Teodora and Smiljanić, Katarina and Prodić, Ivana and Ćirković Veličković, Tanja",
year = "2023",
abstract = "A bottom-up proteomic study, using high-resolution tandem mass spectrometry (HRMS) and, PEAKS Studio X+ was performed to investigate the impact of peanut roasting on readily soluble allergens and their post-translational modification (PTM) profiles. Among four major peanut allergen groups, we found that Ara h 3 prevails in raw peanut extract (PE), similar to Ara h 1, while the opposite is true for Ara h 6, which is enriched in roasted PE; Ara h 2 bands are near the same intensity. HRMS detected more than 40 different types of modification in raw and roasted samples. Distinct variations in the types and occurrence of specific amino acid PTMs were identified between allergens present in raw and roasted samples. Roasting affected the most frequent modifications by enrichment of Ox, HyP, carbamoylation (KR), and deamidation. The PMs could also be mapped to the regions of IgE-binding epitopes of Ara h 1-3 and Ara h 6. As porcine trypsin is used for HRMS sample preparation and is also a digestive protease, hindrance effects to trypsin efficacy regarding PTMs were assessed. Roasting caused dihydroxylation and formylation PTMs with hindrance effects to trypsin efficacy, while methylation on several K/R showed opposite effects. In the case of methylated R342, results suggested facilitation of tryptic performance at modified residue compared to unmodified counterpart, while in the rest of the seven genuine sequences containing modified residues, the trend was opposite. Further exploration of how different PTMs could affect digestion efficiencies of major gastric and intestinal peptidases is currently in works and a much-needed assessment to better understand the role of PTMs.",
publisher = "Faculty of Chemistry, Serbian Chemical Society",
journal = "Book of Abstracts, Serbian Biochemical Society Twelfth Conference "Bicehemistry in Biotechnology", September 21st, 2023",
title = "Mass spectrometry analysis reveals impact of peanut roasting on post-translational modifications of key allergens and  their hinderance of trypsin digestion",
pages = "146-146"
}
Đukić, T., Smiljanić, K., Prodić, I.,& Ćirković Veličković, T.. (2023). Mass spectrometry analysis reveals impact of peanut roasting on post-translational modifications of key allergens and  their hinderance of trypsin digestion. in Book of Abstracts, Serbian Biochemical Society Twelfth Conference "Bicehemistry in Biotechnology", September 21st, 2023
Faculty of Chemistry, Serbian Chemical Society., 146-146.
Đukić T, Smiljanić K, Prodić I, Ćirković Veličković T. Mass spectrometry analysis reveals impact of peanut roasting on post-translational modifications of key allergens and  their hinderance of trypsin digestion. in Book of Abstracts, Serbian Biochemical Society Twelfth Conference "Bicehemistry in Biotechnology", September 21st, 2023. 2023;:146-146..
Đukić, Teodora, Smiljanić, Katarina, Prodić, Ivana, Ćirković Veličković, Tanja, "Mass spectrometry analysis reveals impact of peanut roasting on post-translational modifications of key allergens and  their hinderance of trypsin digestion" in Book of Abstracts, Serbian Biochemical Society Twelfth Conference "Bicehemistry in Biotechnology", September 21st, 2023 (2023):146-146.

Supplementary material for: Abdelhameed, S. A. M., de Azambuja, F., Vasović, T., Savić, N. D., Ćirković-Veličković, T.,& Parac-Vogt, T. N.. (2023). Regioselective protein oxidative cleavage enabled by enzyme-like recognition of an inorganic metal oxo cluster ligand. in Nature Communications Springer Nature., 14(1), 486. https://doi.org/10.1038/s41467-023-36085-z

Abdelhameed, Shorok A. M.; de Azambuja, Francisco; Vasović, Tamara; Savić, Nada D.; Ćirković-Veličković, Tanja; Parac-Vogt, Tatjana N.

(Springer Nature, 2023)

TY  - DATA
AU  - Abdelhameed, Shorok A. M.
AU  - de Azambuja, Francisco
AU  - Vasović, Tamara
AU  - Savić, Nada D.
AU  - Ćirković-Veličković, Tanja
AU  - Parac-Vogt, Tatjana N.
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5816
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5840
AB  - Oxidative modifications of proteins are key to many applications in biotechnology. Metal-catalyzed oxidation reactions efficiently oxidize proteins but with low selectivity, and are highly dependent on the protein surface residues to direct the reaction. Herein, we demonstrate that discrete inorganic ligands such as polyoxometalates enable an efficient and selective protein oxidative cleavage. In the presence of ascorbate (1 mM), the Cu-substituted polyoxometalate K8[Cu2+(H2O)(α2-P2W17O61)], (CuIIWD, 0.05 mM) selectively cleave hen egg white lysozyme under physiological conditions (pH =7.5, 37 °C) producing only four bands in the gel electropherogram (12.7, 11, 10, and 5 kDa). Liquid chromatography/mass spectrometry analysis reveals a regioselective cleavage in the vicinity of crystallographic CuIIWD/lysozyme interaction sites. Mechanistically, polyoxometalate is critical to position the Cu at the protein surface and limit the generation of oxidative species to the proximity of binding sites. Ultimately, this study outlines the potential of discrete, designable metal oxo clusters as catalysts for the selective modification of proteins through radical mechanisms under non-denaturing conditions.
PB  - Springer Nature
T2  - Nature Communications
T1  - Supplementary material for: Abdelhameed, S. A. M., de Azambuja, F., Vasović, T., Savić, N. D., Ćirković-Veličković, T.,& Parac-Vogt, T. N.. (2023). Regioselective protein oxidative cleavage enabled by enzyme-like recognition of an inorganic metal oxo cluster ligand. in Nature Communications Springer Nature., 14(1), 486. https://doi.org/10.1038/s41467-023-36085-z
VL  - 14
IS  - 1
SP  - 486
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5840
ER  - 
@misc{
author = "Abdelhameed, Shorok A. M. and de Azambuja, Francisco and Vasović, Tamara and Savić, Nada D. and Ćirković-Veličković, Tanja and Parac-Vogt, Tatjana N.",
year = "2023",
abstract = "Oxidative modifications of proteins are key to many applications in biotechnology. Metal-catalyzed oxidation reactions efficiently oxidize proteins but with low selectivity, and are highly dependent on the protein surface residues to direct the reaction. Herein, we demonstrate that discrete inorganic ligands such as polyoxometalates enable an efficient and selective protein oxidative cleavage. In the presence of ascorbate (1 mM), the Cu-substituted polyoxometalate K8[Cu2+(H2O)(α2-P2W17O61)], (CuIIWD, 0.05 mM) selectively cleave hen egg white lysozyme under physiological conditions (pH =7.5, 37 °C) producing only four bands in the gel electropherogram (12.7, 11, 10, and 5 kDa). Liquid chromatography/mass spectrometry analysis reveals a regioselective cleavage in the vicinity of crystallographic CuIIWD/lysozyme interaction sites. Mechanistically, polyoxometalate is critical to position the Cu at the protein surface and limit the generation of oxidative species to the proximity of binding sites. Ultimately, this study outlines the potential of discrete, designable metal oxo clusters as catalysts for the selective modification of proteins through radical mechanisms under non-denaturing conditions.",
publisher = "Springer Nature",
journal = "Nature Communications",
title = "Supplementary material for: Abdelhameed, S. A. M., de Azambuja, F., Vasović, T., Savić, N. D., Ćirković-Veličković, T.,& Parac-Vogt, T. N.. (2023). Regioselective protein oxidative cleavage enabled by enzyme-like recognition of an inorganic metal oxo cluster ligand. in Nature Communications Springer Nature., 14(1), 486. https://doi.org/10.1038/s41467-023-36085-z",
volume = "14",
number = "1",
pages = "486",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5840"
}
Abdelhameed, S. A. M., de Azambuja, F., Vasović, T., Savić, N. D., Ćirković-Veličković, T.,& Parac-Vogt, T. N.. (2023). Supplementary material for: Abdelhameed, S. A. M., de Azambuja, F., Vasović, T., Savić, N. D., Ćirković-Veličković, T.,& Parac-Vogt, T. N.. (2023). Regioselective protein oxidative cleavage enabled by enzyme-like recognition of an inorganic metal oxo cluster ligand. in Nature Communications Springer Nature., 14(1), 486. https://doi.org/10.1038/s41467-023-36085-z. in Nature Communications
Springer Nature., 14(1), 486.
https://hdl.handle.net/21.15107/rcub_cherry_5840
Abdelhameed SAM, de Azambuja F, Vasović T, Savić ND, Ćirković-Veličković T, Parac-Vogt TN. Supplementary material for: Abdelhameed, S. A. M., de Azambuja, F., Vasović, T., Savić, N. D., Ćirković-Veličković, T.,& Parac-Vogt, T. N.. (2023). Regioselective protein oxidative cleavage enabled by enzyme-like recognition of an inorganic metal oxo cluster ligand. in Nature Communications Springer Nature., 14(1), 486. https://doi.org/10.1038/s41467-023-36085-z. in Nature Communications. 2023;14(1):486.
https://hdl.handle.net/21.15107/rcub_cherry_5840 .
Abdelhameed, Shorok A. M., de Azambuja, Francisco, Vasović, Tamara, Savić, Nada D., Ćirković-Veličković, Tanja, Parac-Vogt, Tatjana N., "Supplementary material for: Abdelhameed, S. A. M., de Azambuja, F., Vasović, T., Savić, N. D., Ćirković-Veličković, T.,& Parac-Vogt, T. N.. (2023). Regioselective protein oxidative cleavage enabled by enzyme-like recognition of an inorganic metal oxo cluster ligand. in Nature Communications Springer Nature., 14(1), 486. https://doi.org/10.1038/s41467-023-36085-z" in Nature Communications, 14, no. 1 (2023):486,
https://hdl.handle.net/21.15107/rcub_cherry_5840 .

Comparative digestion of thermally treated vertebrates and invertebrates allergen pairs in real food matrix

Khulal, Urmila; Stojadinović, Marija M.; Prodić, Ivana; Rajković, Andrea; Ćirković-Veličković, Tanja

(Elsevier, 2023)

TY  - JOUR
AU  - Khulal, Urmila
AU  - Stojadinović, Marija M.
AU  - Prodić, Ivana
AU  - Rajković, Andrea
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5765
AB  - The digestion stability of allergen pairs, tropomyosin, TM (fish and seafood allergen), and myosin light chain, MLC (chicken meat allergen) is compared among vertebrates and invertebrates in raw and cooked food matrix under standardized simulated in vitro gastrointestinal (GI) digestion. SDS-PAGE followed by multiple TM and MLC-specific antibodies in semidry WB revealed pepsin resistance of invertebrate TMs (abalone, oyster, shrimp) under diet-relevant conditions (raw, cooked). Vertebrate TMs (chicken, pork, beef) were less stable to digestion except that the raw chicken TM was observed pepsin resistant (not diet-relevant). Vertebrate (chicken) MLC was thermally stable. A new 28 kDa protein bound to anti-MLC antibody in cooked chicken and pork; could be the aggregates of MLC. Raw shrimp MLC showed pepsin resistance among invertebrates. A good correlation was observed between combined resistance of TM and MLC to GI digestion following the diet-relevant thermal treatment and reported protein allergenicity among vertebrates and invertebrates.
PB  - Elsevier
T2  - Food Chemistry
T1  - Comparative digestion of thermally treated vertebrates and invertebrates allergen pairs in real food matrix
VL  - 405
SP  - 134981
DO  - 10.1016/j.foodchem.2022.134981
ER  - 
@article{
author = "Khulal, Urmila and Stojadinović, Marija M. and Prodić, Ivana and Rajković, Andrea and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "The digestion stability of allergen pairs, tropomyosin, TM (fish and seafood allergen), and myosin light chain, MLC (chicken meat allergen) is compared among vertebrates and invertebrates in raw and cooked food matrix under standardized simulated in vitro gastrointestinal (GI) digestion. SDS-PAGE followed by multiple TM and MLC-specific antibodies in semidry WB revealed pepsin resistance of invertebrate TMs (abalone, oyster, shrimp) under diet-relevant conditions (raw, cooked). Vertebrate TMs (chicken, pork, beef) were less stable to digestion except that the raw chicken TM was observed pepsin resistant (not diet-relevant). Vertebrate (chicken) MLC was thermally stable. A new 28 kDa protein bound to anti-MLC antibody in cooked chicken and pork; could be the aggregates of MLC. Raw shrimp MLC showed pepsin resistance among invertebrates. A good correlation was observed between combined resistance of TM and MLC to GI digestion following the diet-relevant thermal treatment and reported protein allergenicity among vertebrates and invertebrates.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Comparative digestion of thermally treated vertebrates and invertebrates allergen pairs in real food matrix",
volume = "405",
pages = "134981",
doi = "10.1016/j.foodchem.2022.134981"
}
Khulal, U., Stojadinović, M. M., Prodić, I., Rajković, A.,& Ćirković-Veličković, T.. (2023). Comparative digestion of thermally treated vertebrates and invertebrates allergen pairs in real food matrix. in Food Chemistry
Elsevier., 405, 134981.
https://doi.org/10.1016/j.foodchem.2022.134981
Khulal U, Stojadinović MM, Prodić I, Rajković A, Ćirković-Veličković T. Comparative digestion of thermally treated vertebrates and invertebrates allergen pairs in real food matrix. in Food Chemistry. 2023;405:134981.
doi:10.1016/j.foodchem.2022.134981 .
Khulal, Urmila, Stojadinović, Marija M., Prodić, Ivana, Rajković, Andrea, Ćirković-Veličković, Tanja, "Comparative digestion of thermally treated vertebrates and invertebrates allergen pairs in real food matrix" in Food Chemistry, 405 (2023):134981,
https://doi.org/10.1016/j.foodchem.2022.134981 . .
2
4
3
2

Occurrence of microplastics in korean mussel (Mytilus unguiculatus) collected from local fish markets in South Korea and identification using micro-FTIR spectroscopy

Mutić, Tamara; Ilić, Miloš; Mutić, Jelena; Ćirković Veličković, Tanja

(Belgrade : Serbian Chemical Society, 2023)

TY  - CONF
AU  - Mutić, Tamara
AU  - Ilić, Miloš
AU  - Mutić, Jelena
AU  - Ćirković Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6520
AB  - Microplastics (MPs) have been considered a new type of pollutant in the marine environment and have attracted widespread attention worldwide in recent years. Plastic
particles with particle size less than 5 mm are defined as MPs [1].
Mussels were used as an indicator of marine environments contamination in biomonitoring programs [2]. The aim of this study was to determine the abundance of MPs in Korean or hard-shelled mussel, one of the most economically and ecologically important bivalves in South Korea, collected from seafood market intended for human consumption. After extensive removal of biological material by alkaline, enzymatic and oxidative digestion, MPs were characterized by micro-FTIR spectroscopy, which is a proven method for the identification of all types of polymers and enables reliable differentiation between substances of natural and synthetic origin. In total, we found 0.53 MPs per individual which is less than 0.3 MPs per gram of soft tissue. Major types of isolated MPs with their size and shape are shown in Figure 1.
PB  - Belgrade : Serbian Chemical Society
PB  - Belgrade : Serbian Young Chemists’ Club
C3  - 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Sciences
T1  - Occurrence of microplastics in korean mussel (Mytilus unguiculatus) collected from local fish markets in South Korea and identification using micro-FTIR spectroscopy
SP  - 86
EP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6520
ER  - 
@conference{
author = "Mutić, Tamara and Ilić, Miloš and Mutić, Jelena and Ćirković Veličković, Tanja",
year = "2023",
abstract = "Microplastics (MPs) have been considered a new type of pollutant in the marine environment and have attracted widespread attention worldwide in recent years. Plastic
particles with particle size less than 5 mm are defined as MPs [1].
Mussels were used as an indicator of marine environments contamination in biomonitoring programs [2]. The aim of this study was to determine the abundance of MPs in Korean or hard-shelled mussel, one of the most economically and ecologically important bivalves in South Korea, collected from seafood market intended for human consumption. After extensive removal of biological material by alkaline, enzymatic and oxidative digestion, MPs were characterized by micro-FTIR spectroscopy, which is a proven method for the identification of all types of polymers and enables reliable differentiation between substances of natural and synthetic origin. In total, we found 0.53 MPs per individual which is less than 0.3 MPs per gram of soft tissue. Major types of isolated MPs with their size and shape are shown in Figure 1.",
publisher = "Belgrade : Serbian Chemical Society, Belgrade : Serbian Young Chemists’ Club",
journal = "9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Sciences",
title = "Occurrence of microplastics in korean mussel (Mytilus unguiculatus) collected from local fish markets in South Korea and identification using micro-FTIR spectroscopy",
pages = "86-86",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6520"
}
Mutić, T., Ilić, M., Mutić, J.,& Ćirković Veličković, T.. (2023). Occurrence of microplastics in korean mussel (Mytilus unguiculatus) collected from local fish markets in South Korea and identification using micro-FTIR spectroscopy. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Sciences
Belgrade : Serbian Chemical Society., 86-86.
https://hdl.handle.net/21.15107/rcub_cherry_6520
Mutić T, Ilić M, Mutić J, Ćirković Veličković T. Occurrence of microplastics in korean mussel (Mytilus unguiculatus) collected from local fish markets in South Korea and identification using micro-FTIR spectroscopy. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Sciences. 2023;:86-86.
https://hdl.handle.net/21.15107/rcub_cherry_6520 .
Mutić, Tamara, Ilić, Miloš, Mutić, Jelena, Ćirković Veličković, Tanja, "Occurrence of microplastics in korean mussel (Mytilus unguiculatus) collected from local fish markets in South Korea and identification using micro-FTIR spectroscopy" in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Sciences (2023):86-86,
https://hdl.handle.net/21.15107/rcub_cherry_6520 .

Chemical characterization and quantification of microplastics particles from mussel samples based on Micro-FTIR spectroscopy

Mutić, Tamara; Anđelković, Boban; Stanić-Vučinić, Dragana; Turkalj, Mirjana; Ilić, Miloš; Ćirković Veličković, Tanja

(Belgrade : Serbian Chemical Society, 2023)

TY  - CONF
AU  - Mutić, Tamara
AU  - Anđelković, Boban
AU  - Stanić-Vučinić, Dragana
AU  - Turkalj, Mirjana
AU  - Ilić, Miloš
AU  - Ćirković Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6519
AB  - Seafood has been widely recognized as an integral part of a balanced and healthy diet, thanks to its high nutritional value and associated multiple health benefits. Seafood contamination with microplastics (MPs) have recently been highlighted as an emerging concern for global food security.1
According to definition, polymer particles with a diameter of less than 5 mm are referred to as microplastics particles (MPs)2. Most bivalves, as well as mussels, are filter feeders and can accumulate particles in their bodies. By consuming seafood, MP particles can be included in the human food chain. Most of the studies analyzed MPs content in environmental samples, instead of point-of-sales samples.
The aim of this study was to determine the abundance of microplastics in mussels collected from Croatia seafood market intended for human consumption. 5 clean laboratory criteria (100% cotton lab coat, clean glassware, filtered solutions, rinsed the outside of animals before dissection, and limited air exposure of samples) were fulfilled by our study. Digestion and manipulation of the sample was conducted in a clean hood. Procedural controls were included for each measurement. After extensive removal of biological material by alkaline, enzymatic and oxidation digestion, MPs were characterized by micro-Fourier-transform infrared spectroscopy (Micro-FTIR). It is a proven method for the identification of all types of polymers and allows a reliable differentiation between substances of natural and synthetic origin. Our approach included one individual per sample for micro-FTIR analysis, no sub-grouping of the sample for chemical characterization (100% of particles isolated from each sample have been analyzed by micro- FTIR) and manual confirmation of all recorded spectra with more than 60% match to the polymer material in the spectral library (Thermo-Fisher; using the OMNIC PictaTM software). Soft tissue from inside the shell was not extensively washed to allow estimation of MPs intake during consumption.
Almost half of total samples (sample size=27) did not contain microplastic particles (52%). The highest number of particles per individual was 4 (Fig. 1). In total, we found 23 MPs particles in 27 individuals or 0.85 MPs per individual, range 0-4, and less than 0.3 MPs/g of soft tissue. In this research, major types of isolated MPs were polystyrene
(PS), poly(ethylene terephthalate) (PET), polypropylene (PP) and polyethylene (PE), shown in Table 1. PE and PS are the most popular plastic materials used in consumer products and they have shorter service lives than other types of plastics.
PB  - Belgrade : Serbian Chemical Society
C3  - Book of Abstracts of the XXII EuroFoodChem Congress, Belgrade, Serbia, 14-16 June 2023
T1  - Chemical characterization and quantification of microplastics particles from mussel samples based on Micro-FTIR spectroscopy
SP  - 281
EP  - 281
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6519
ER  - 
@conference{
author = "Mutić, Tamara and Anđelković, Boban and Stanić-Vučinić, Dragana and Turkalj, Mirjana and Ilić, Miloš and Ćirković Veličković, Tanja",
year = "2023",
abstract = "Seafood has been widely recognized as an integral part of a balanced and healthy diet, thanks to its high nutritional value and associated multiple health benefits. Seafood contamination with microplastics (MPs) have recently been highlighted as an emerging concern for global food security.1
According to definition, polymer particles with a diameter of less than 5 mm are referred to as microplastics particles (MPs)2. Most bivalves, as well as mussels, are filter feeders and can accumulate particles in their bodies. By consuming seafood, MP particles can be included in the human food chain. Most of the studies analyzed MPs content in environmental samples, instead of point-of-sales samples.
The aim of this study was to determine the abundance of microplastics in mussels collected from Croatia seafood market intended for human consumption. 5 clean laboratory criteria (100% cotton lab coat, clean glassware, filtered solutions, rinsed the outside of animals before dissection, and limited air exposure of samples) were fulfilled by our study. Digestion and manipulation of the sample was conducted in a clean hood. Procedural controls were included for each measurement. After extensive removal of biological material by alkaline, enzymatic and oxidation digestion, MPs were characterized by micro-Fourier-transform infrared spectroscopy (Micro-FTIR). It is a proven method for the identification of all types of polymers and allows a reliable differentiation between substances of natural and synthetic origin. Our approach included one individual per sample for micro-FTIR analysis, no sub-grouping of the sample for chemical characterization (100% of particles isolated from each sample have been analyzed by micro- FTIR) and manual confirmation of all recorded spectra with more than 60% match to the polymer material in the spectral library (Thermo-Fisher; using the OMNIC PictaTM software). Soft tissue from inside the shell was not extensively washed to allow estimation of MPs intake during consumption.
Almost half of total samples (sample size=27) did not contain microplastic particles (52%). The highest number of particles per individual was 4 (Fig. 1). In total, we found 23 MPs particles in 27 individuals or 0.85 MPs per individual, range 0-4, and less than 0.3 MPs/g of soft tissue. In this research, major types of isolated MPs were polystyrene
(PS), poly(ethylene terephthalate) (PET), polypropylene (PP) and polyethylene (PE), shown in Table 1. PE and PS are the most popular plastic materials used in consumer products and they have shorter service lives than other types of plastics.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Book of Abstracts of the XXII EuroFoodChem Congress, Belgrade, Serbia, 14-16 June 2023",
title = "Chemical characterization and quantification of microplastics particles from mussel samples based on Micro-FTIR spectroscopy",
pages = "281-281",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6519"
}
Mutić, T., Anđelković, B., Stanić-Vučinić, D., Turkalj, M., Ilić, M.,& Ćirković Veličković, T.. (2023). Chemical characterization and quantification of microplastics particles from mussel samples based on Micro-FTIR spectroscopy. in Book of Abstracts of the XXII EuroFoodChem Congress, Belgrade, Serbia, 14-16 June 2023
Belgrade : Serbian Chemical Society., 281-281.
https://hdl.handle.net/21.15107/rcub_cherry_6519
Mutić T, Anđelković B, Stanić-Vučinić D, Turkalj M, Ilić M, Ćirković Veličković T. Chemical characterization and quantification of microplastics particles from mussel samples based on Micro-FTIR spectroscopy. in Book of Abstracts of the XXII EuroFoodChem Congress, Belgrade, Serbia, 14-16 June 2023. 2023;:281-281.
https://hdl.handle.net/21.15107/rcub_cherry_6519 .
Mutić, Tamara, Anđelković, Boban, Stanić-Vučinić, Dragana, Turkalj, Mirjana, Ilić, Miloš, Ćirković Veličković, Tanja, "Chemical characterization and quantification of microplastics particles from mussel samples based on Micro-FTIR spectroscopy" in Book of Abstracts of the XXII EuroFoodChem Congress, Belgrade, Serbia, 14-16 June 2023 (2023):281-281,
https://hdl.handle.net/21.15107/rcub_cherry_6519 .

Food allergen bio-corona on microplastics

Ćirković-Veličković, Tanja; Stanić-Vučinić, Dragana

(TNO and UMC Utrecht, the Netherlands, 2023)

TY  - CONF
AU  - Ćirković-Veličković, Tanja
AU  - Stanić-Vučinić, Dragana
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6522
AB  - Human exposure to nano- and microplastics (NMPs) is a reality, and contamination of food with NMPs
has become an issue worldwide. Due to their small size and pervasive nature, NMPs may enter the
human body through various routes, ingestion being the most relevant route for food and beverages
contaminated with NMPs. The presence of NMPs in the GIT allows them to reach different parts of the
body, where they may have both physical and chemical effects.
GIT is also the first point of contact of food allergens and NMPs. To clarify effects of food allergens
adsorption and bio-corona formation on NMPs, we have examined binding and structural changes of
several allergenic food proteins (ovalbumine, beta-lactoglobuline, tropomyosine) to the polystyrene,
polypropylene, and polyethylene terephthalate microplastics. Our results show high binding affinity of
food allergens to the plastic particles and structural and functional changes in the allergenic food
proteins bound in soft and hard corona. Furthermore, presence of NMPs in the digestive fluid may
influence digestion of allergenic food proteins. Our study on the pepsin digestion of milk proteins in the
presence of PS showed that cow’s milk proteins (caseins) and their larger fragments preferentially bind
to the hard corona of PS-MPs during the gastric digestion of milk leading to prolonged survival of larger
peptides (particularly of αS2-casein) in the simulated gastric fluid. In future studies, it will be particularly
relevant to further study impact of nanoplastics on allergenic proteins digestion and bioavailability.
Larger surface area of nanoplastics in comparison to microplastics may provide more anchoring points
for proteins and digestive enzymes to tightly bind in the hard corona, causing structural changes and
possibly impacting digestive enzymes’ function. In the future, particular attention should be given to
infant, elderly, and groups already showing digestion impairment, as most of the studies conducted so
far consider only healthy adult digestion. However, data so far obtained at the molecular level suggest
adsorption and structural changes in allergenic food proteins, particularly in bio-corona around NMPs
and impairment of function of digestive enzymes. Studies are warranted on the impact of NMPs/allergen
co-exposure on allergenicity and sensitizing potential of allergenic foods contaminated with NMP to fully
understand possible impact of NMPs on development and severity of food allergies.
PB  - TNO and UMC Utrecht, the Netherlands
C3  - The 3rd international conference: Food Allergy Forum - Towards a Food Allergy-Free World, Netherland, Amsterdam, September 27-29, 2023
T1  - Food allergen bio-corona on microplastics
SP  - 17
EP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6522
ER  - 
@conference{
author = "Ćirković-Veličković, Tanja and Stanić-Vučinić, Dragana",
year = "2023",
abstract = "Human exposure to nano- and microplastics (NMPs) is a reality, and contamination of food with NMPs
has become an issue worldwide. Due to their small size and pervasive nature, NMPs may enter the
human body through various routes, ingestion being the most relevant route for food and beverages
contaminated with NMPs. The presence of NMPs in the GIT allows them to reach different parts of the
body, where they may have both physical and chemical effects.
GIT is also the first point of contact of food allergens and NMPs. To clarify effects of food allergens
adsorption and bio-corona formation on NMPs, we have examined binding and structural changes of
several allergenic food proteins (ovalbumine, beta-lactoglobuline, tropomyosine) to the polystyrene,
polypropylene, and polyethylene terephthalate microplastics. Our results show high binding affinity of
food allergens to the plastic particles and structural and functional changes in the allergenic food
proteins bound in soft and hard corona. Furthermore, presence of NMPs in the digestive fluid may
influence digestion of allergenic food proteins. Our study on the pepsin digestion of milk proteins in the
presence of PS showed that cow’s milk proteins (caseins) and their larger fragments preferentially bind
to the hard corona of PS-MPs during the gastric digestion of milk leading to prolonged survival of larger
peptides (particularly of αS2-casein) in the simulated gastric fluid. In future studies, it will be particularly
relevant to further study impact of nanoplastics on allergenic proteins digestion and bioavailability.
Larger surface area of nanoplastics in comparison to microplastics may provide more anchoring points
for proteins and digestive enzymes to tightly bind in the hard corona, causing structural changes and
possibly impacting digestive enzymes’ function. In the future, particular attention should be given to
infant, elderly, and groups already showing digestion impairment, as most of the studies conducted so
far consider only healthy adult digestion. However, data so far obtained at the molecular level suggest
adsorption and structural changes in allergenic food proteins, particularly in bio-corona around NMPs
and impairment of function of digestive enzymes. Studies are warranted on the impact of NMPs/allergen
co-exposure on allergenicity and sensitizing potential of allergenic foods contaminated with NMP to fully
understand possible impact of NMPs on development and severity of food allergies.",
publisher = "TNO and UMC Utrecht, the Netherlands",
journal = "The 3rd international conference: Food Allergy Forum - Towards a Food Allergy-Free World, Netherland, Amsterdam, September 27-29, 2023",
title = "Food allergen bio-corona on microplastics",
pages = "17-17",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6522"
}
Ćirković-Veličković, T.,& Stanić-Vučinić, D.. (2023). Food allergen bio-corona on microplastics. in The 3rd international conference: Food Allergy Forum - Towards a Food Allergy-Free World, Netherland, Amsterdam, September 27-29, 2023
TNO and UMC Utrecht, the Netherlands., 17-17.
https://hdl.handle.net/21.15107/rcub_cherry_6522
Ćirković-Veličković T, Stanić-Vučinić D. Food allergen bio-corona on microplastics. in The 3rd international conference: Food Allergy Forum - Towards a Food Allergy-Free World, Netherland, Amsterdam, September 27-29, 2023. 2023;:17-17.
https://hdl.handle.net/21.15107/rcub_cherry_6522 .
Ćirković-Veličković, Tanja, Stanić-Vučinić, Dragana, "Food allergen bio-corona on microplastics" in The 3rd international conference: Food Allergy Forum - Towards a Food Allergy-Free World, Netherland, Amsterdam, September 27-29, 2023 (2023):17-17,
https://hdl.handle.net/21.15107/rcub_cherry_6522 .

Research data no.1 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703

Đapović, Milica; Apostolović, Danijela; Postić, Vojislava; Lujić, Tamara; Jovanović, Vesna; Stanić-Vučinić, Dragana; Van Hage, Marianne; Maslak, Veselin; Ćirković-Veličković, Tanja

(MDPI, 2023)

TY  - DATA
AU  - Đapović, Milica
AU  - Apostolović, Danijela
AU  - Postić, Vojislava
AU  - Lujić, Tamara
AU  - Jovanović, Vesna
AU  - Stanić-Vučinić, Dragana
AU  - Van Hage, Marianne
AU  - Maslak, Veselin
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6460
AB  - Percentage of hemolysis of RBC in the presence of washed PET NPs and
unwashed NPs (with SDS) determined by incubation of human red blood cells (RBCs) from
three healthy donors. Output from the  Shimadzu UV/ViS 1800 (Kyoto, Japan) nspectrometer - raw spectral data and the calculation of percentages of hemolysis (procesesd data).
PB  - MDPI
T2  - Polymers
T1  - Research data no.1 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers
MDPI., 15(24), 4703.
https://doi.org/10.3390/polym15244703
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6460
ER  - 
@misc{
author = "Đapović, Milica and Apostolović, Danijela and Postić, Vojislava and Lujić, Tamara and Jovanović, Vesna and Stanić-Vučinić, Dragana and Van Hage, Marianne and Maslak, Veselin and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "Percentage of hemolysis of RBC in the presence of washed PET NPs and
unwashed NPs (with SDS) determined by incubation of human red blood cells (RBCs) from
three healthy donors. Output from the  Shimadzu UV/ViS 1800 (Kyoto, Japan) nspectrometer - raw spectral data and the calculation of percentages of hemolysis (procesesd data).",
publisher = "MDPI",
journal = "Polymers",
title = "Research data no.1 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers
MDPI., 15(24), 4703.
https://doi.org/10.3390/polym15244703",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6460"
}
Đapović, M., Apostolović, D., Postić, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., Van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Research data no.1 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers
MDPI., 15(24), 4703.
https://doi.org/10.3390/polym15244703. in Polymers
MDPI..
https://hdl.handle.net/21.15107/rcub_cherry_6460
Đapović M, Apostolović D, Postić V, Lujić T, Jovanović V, Stanić-Vučinić D, Van Hage M, Maslak V, Ćirković-Veličković T. Research data no.1 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers
MDPI., 15(24), 4703.
https://doi.org/10.3390/polym15244703. in Polymers. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6460 .
Đapović, Milica, Apostolović, Danijela, Postić, Vojislava, Lujić, Tamara, Jovanović, Vesna, Stanić-Vučinić, Dragana, Van Hage, Marianne, Maslak, Veselin, Ćirković-Veličković, Tanja, "Research data no.1 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers
MDPI., 15(24), 4703.
https://doi.org/10.3390/polym15244703" in Polymers (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6460 .

Isolation of microplastic particles from samples from the marine ecosystem and characterization using μFTIR spectroscopy

Mutić, Tamara; Anđelković, Boban; Ilić, Miloš; Mutić, Jelena; Ćirković-Veličković, Tanja

(Beograd : Srpsko hemijsko društvo, 2023)

TY  - CONF
AU  - Mutić, Tamara
AU  - Anđelković, Boban
AU  - Ilić, Miloš
AU  - Mutić, Jelena
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6513
AB  - Tokom protekle decenije došlo je do velikog naučnog i javnog interesovanja za uticaj plastike na životnu sredinu, žive organizme, uključujući ljude i živi svet u morskim ekosistemima. [1]
 Izdvajanje plastičnih čestica iz uzorka radi njihove detekcije i dalje karakterizacije predstavlja veliki izazov jer su često pomešne sa velikim količinama mineralnih i biogenih organskih supstanci. Primenjeni protokoli treba da ostave intaktne sintetičke polimere prvenstveno u pogledu njihove hemijske strukture, a zatim i u pogledu njihove težine, zapremine, oblika i, ako je potrebno, boje. Očuvanje hemijske strukture ili površinske strukture i morfologije polimera u velikoj meri zavisi od odabira adekvatne metode digestije. [2]
 U ovom istraživanju ekstrakcija mikroplastike (MP) iz morskih organizama se zasnivala na višestepenoj digestiji, kao i na kombinovanju hemijske i enzimske digestije. Prvi stepen je hemijska digestija, koja podrazumeva upotrebu jakih alkalnih uslova sredine (KOH koncentracije 10 %), a drugi stepen ekstrakcije je digestija sa dva enzima, prvo pepsinom i zatim pankreatinom. Poslednji korak koji je od velike važnosti za dalja spekralna ispitivanja je bila upotreba oksidativnih reagenasa, odnosno vodonik peroksida, koncentracije 15 %.
 Analizirani su standardi čestica MP vrste PP veličine 500 μm, PVC veličine 120 μm, PS veličine 500 μm i PET veličine 120 μm. Čestice su dodate pre digestije (spike), a nakon što su prošle digestiju po optimizovanom protokolu su analizirane primenom μFTIR- spektrometra.
 Kod čestica PP, PVC i PS ne dolazi do promene strukture i nalažene su čestice sa velikim procentom podudaranja nakon digestije. Rezultati prikazani u Tabeli 1 pokazuju da se određeni tipovi plastike izloženi hemijskoj i enzimskoj digestiji, kao i eventualnom taloženju biološkog materijala, identifikuju sa manjim stepenom podudaranja sa bazama podataka, nego pre tretmana. I pored toga, procenat podudaranja sa bazama je iznosio za PP do 90 %, za PVC do 94 %, za PS do 68 % i za PET do 70 %.
Podaci prikazani na slici 1 predstavljaju šematski prikaz toka analize koja uključuje višestepenu analizu uzoraka, prebacivanje digestovane materije sa mikroplastičnim česticama i karakterizaciju μFTIR spektrometrom.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 9th Symposium Chemistry and Environmental Protection EnviroChem2023 with International Participation, Book of Abstracts, Kladovo, June 4-7, 2023
T1  - Isolation of microplastic particles from samples from the marine ecosystem and characterization using μFTIR spectroscopy
T1  - Izolovanje mikroplastičnih čestica iz uzoraka poreklom iz morskog ekosistema
i karakterizacija primenom µFTIR spektroskopije
SP  - 89
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6513
ER  - 
@conference{
author = "Mutić, Tamara and Anđelković, Boban and Ilić, Miloš and Mutić, Jelena and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "Tokom protekle decenije došlo je do velikog naučnog i javnog interesovanja za uticaj plastike na životnu sredinu, žive organizme, uključujući ljude i živi svet u morskim ekosistemima. [1]
 Izdvajanje plastičnih čestica iz uzorka radi njihove detekcije i dalje karakterizacije predstavlja veliki izazov jer su često pomešne sa velikim količinama mineralnih i biogenih organskih supstanci. Primenjeni protokoli treba da ostave intaktne sintetičke polimere prvenstveno u pogledu njihove hemijske strukture, a zatim i u pogledu njihove težine, zapremine, oblika i, ako je potrebno, boje. Očuvanje hemijske strukture ili površinske strukture i morfologije polimera u velikoj meri zavisi od odabira adekvatne metode digestije. [2]
 U ovom istraživanju ekstrakcija mikroplastike (MP) iz morskih organizama se zasnivala na višestepenoj digestiji, kao i na kombinovanju hemijske i enzimske digestije. Prvi stepen je hemijska digestija, koja podrazumeva upotrebu jakih alkalnih uslova sredine (KOH koncentracije 10 %), a drugi stepen ekstrakcije je digestija sa dva enzima, prvo pepsinom i zatim pankreatinom. Poslednji korak koji je od velike važnosti za dalja spekralna ispitivanja je bila upotreba oksidativnih reagenasa, odnosno vodonik peroksida, koncentracije 15 %.
 Analizirani su standardi čestica MP vrste PP veličine 500 μm, PVC veličine 120 μm, PS veličine 500 μm i PET veličine 120 μm. Čestice su dodate pre digestije (spike), a nakon što su prošle digestiju po optimizovanom protokolu su analizirane primenom μFTIR- spektrometra.
 Kod čestica PP, PVC i PS ne dolazi do promene strukture i nalažene su čestice sa velikim procentom podudaranja nakon digestije. Rezultati prikazani u Tabeli 1 pokazuju da se određeni tipovi plastike izloženi hemijskoj i enzimskoj digestiji, kao i eventualnom taloženju biološkog materijala, identifikuju sa manjim stepenom podudaranja sa bazama podataka, nego pre tretmana. I pored toga, procenat podudaranja sa bazama je iznosio za PP do 90 %, za PVC do 94 %, za PS do 68 % i za PET do 70 %.
Podaci prikazani na slici 1 predstavljaju šematski prikaz toka analize koja uključuje višestepenu analizu uzoraka, prebacivanje digestovane materije sa mikroplastičnim česticama i karakterizaciju μFTIR spektrometrom.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "9th Symposium Chemistry and Environmental Protection EnviroChem2023 with International Participation, Book of Abstracts, Kladovo, June 4-7, 2023",
title = "Isolation of microplastic particles from samples from the marine ecosystem and characterization using μFTIR spectroscopy, Izolovanje mikroplastičnih čestica iz uzoraka poreklom iz morskog ekosistema
i karakterizacija primenom µFTIR spektroskopije",
pages = "89-90",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6513"
}
Mutić, T., Anđelković, B., Ilić, M., Mutić, J.,& Ćirković-Veličković, T.. (2023). Isolation of microplastic particles from samples from the marine ecosystem and characterization using μFTIR spectroscopy. in 9th Symposium Chemistry and Environmental Protection EnviroChem2023 with International Participation, Book of Abstracts, Kladovo, June 4-7, 2023
Beograd : Srpsko hemijsko društvo., 89-90.
https://hdl.handle.net/21.15107/rcub_cherry_6513
Mutić T, Anđelković B, Ilić M, Mutić J, Ćirković-Veličković T. Isolation of microplastic particles from samples from the marine ecosystem and characterization using μFTIR spectroscopy. in 9th Symposium Chemistry and Environmental Protection EnviroChem2023 with International Participation, Book of Abstracts, Kladovo, June 4-7, 2023. 2023;:89-90.
https://hdl.handle.net/21.15107/rcub_cherry_6513 .
Mutić, Tamara, Anđelković, Boban, Ilić, Miloš, Mutić, Jelena, Ćirković-Veličković, Tanja, "Isolation of microplastic particles from samples from the marine ecosystem and characterization using μFTIR spectroscopy" in 9th Symposium Chemistry and Environmental Protection EnviroChem2023 with International Participation, Book of Abstracts, Kladovo, June 4-7, 2023 (2023):89-90,
https://hdl.handle.net/21.15107/rcub_cherry_6513 .

Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion

Krishna de Guzman, Maria; Stanić-Vučinić, Dragana; Gligorijević, Nikola; Wimmer, Lukas; Gasparyan, Manvel; Lujić, Tamara; Vasović, Tamara; Dailey, Lea Ann; Van Haute, Sam; Ćirković-Veličković, Tanja

(Elsevier, 2023)

TY  - JOUR
AU  - Krishna de Guzman, Maria
AU  - Stanić-Vučinić, Dragana
AU  - Gligorijević, Nikola
AU  - Wimmer, Lukas
AU  - Gasparyan, Manvel
AU  - Lujić, Tamara
AU  - Vasović, Tamara
AU  - Dailey, Lea Ann
AU  - Van Haute, Sam
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6320
AB  - Human ingestion of microplastics (MPs) is common and inevitable due to the widespread contamination of food items, but implications on the gastric digestion of food proteins are still unknown. In this study, the interactions between pepsin and polystyrene (PS) MPs were evaluated by investigating enzyme activity and conformation in a simulated human gastric environment in the presence or absence of PS MPs. The impact on food digestion was also assessed by monitoring the kinetics of protein hydrolysis through static in vitro gastric digestion of cow's milk contaminated with PS. The binding of pepsin to PS showed that the surface chemistry of MPs dictates binding affinity. The key contributor to pepsin adsorption seems to be π−π interactions between the aromatic residues and the PS phenyl rings. During quick exposure (10 min) of pepsin to increasing concentrations (222, 2219, 22188 particles/mL) of 10 μm PS (PS10) and 100 μm PS (PS100), total enzymatic activities were not affected remarkably. However, upon prolonged exposure at 1 and 2 h, preferential binding of pepsin to the small, low zeta-potential PS caused structural changes in the protein which led to a significant reduction of its activity. Digestion of cow's milk mixed with PS10 resulted in transient accumulation of larger peptides (10–35 kDa) and reduced bioavailability of short peptides (2–9 kDa) in the gastric phase. This, however, was only observed at extremely high PS10 concentration (0.3 mg/mL or 5.46E+05 particles/mL). The digestion of milk peptides, bound preferentially over pepsin within the hard corona on the PS10 surface, was delayed up to 15 min in comparison to bulk protein digestion. Intact caseins, otherwise rapidly digested, remained bound to PS10 in the hard corona for up to 15 min. This work presents valuable insights regarding the interaction of MPs, food proteins, and pepsin, and their dynamics during gastric digestion.
PB  - Elsevier
T2  - Environmental Pollution
T1  - Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion
VL  - 335
SP  - 122282
DO  - 10.1016/j.envpol.2023.122282
ER  - 
@article{
author = "Krishna de Guzman, Maria and Stanić-Vučinić, Dragana and Gligorijević, Nikola and Wimmer, Lukas and Gasparyan, Manvel and Lujić, Tamara and Vasović, Tamara and Dailey, Lea Ann and Van Haute, Sam and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "Human ingestion of microplastics (MPs) is common and inevitable due to the widespread contamination of food items, but implications on the gastric digestion of food proteins are still unknown. In this study, the interactions between pepsin and polystyrene (PS) MPs were evaluated by investigating enzyme activity and conformation in a simulated human gastric environment in the presence or absence of PS MPs. The impact on food digestion was also assessed by monitoring the kinetics of protein hydrolysis through static in vitro gastric digestion of cow's milk contaminated with PS. The binding of pepsin to PS showed that the surface chemistry of MPs dictates binding affinity. The key contributor to pepsin adsorption seems to be π−π interactions between the aromatic residues and the PS phenyl rings. During quick exposure (10 min) of pepsin to increasing concentrations (222, 2219, 22188 particles/mL) of 10 μm PS (PS10) and 100 μm PS (PS100), total enzymatic activities were not affected remarkably. However, upon prolonged exposure at 1 and 2 h, preferential binding of pepsin to the small, low zeta-potential PS caused structural changes in the protein which led to a significant reduction of its activity. Digestion of cow's milk mixed with PS10 resulted in transient accumulation of larger peptides (10–35 kDa) and reduced bioavailability of short peptides (2–9 kDa) in the gastric phase. This, however, was only observed at extremely high PS10 concentration (0.3 mg/mL or 5.46E+05 particles/mL). The digestion of milk peptides, bound preferentially over pepsin within the hard corona on the PS10 surface, was delayed up to 15 min in comparison to bulk protein digestion. Intact caseins, otherwise rapidly digested, remained bound to PS10 in the hard corona for up to 15 min. This work presents valuable insights regarding the interaction of MPs, food proteins, and pepsin, and their dynamics during gastric digestion.",
publisher = "Elsevier",
journal = "Environmental Pollution",
title = "Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion",
volume = "335",
pages = "122282",
doi = "10.1016/j.envpol.2023.122282"
}
Krishna de Guzman, M., Stanić-Vučinić, D., Gligorijević, N., Wimmer, L., Gasparyan, M., Lujić, T., Vasović, T., Dailey, L. A., Van Haute, S.,& Ćirković-Veličković, T.. (2023). Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion. in Environmental Pollution
Elsevier., 335, 122282.
https://doi.org/10.1016/j.envpol.2023.122282
Krishna de Guzman M, Stanić-Vučinić D, Gligorijević N, Wimmer L, Gasparyan M, Lujić T, Vasović T, Dailey LA, Van Haute S, Ćirković-Veličković T. Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion. in Environmental Pollution. 2023;335:122282.
doi:10.1016/j.envpol.2023.122282 .
Krishna de Guzman, Maria, Stanić-Vučinić, Dragana, Gligorijević, Nikola, Wimmer, Lukas, Gasparyan, Manvel, Lujić, Tamara, Vasović, Tamara, Dailey, Lea Ann, Van Haute, Sam, Ćirković-Veličković, Tanja, "Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion" in Environmental Pollution, 335 (2023):122282,
https://doi.org/10.1016/j.envpol.2023.122282 . .
1
3
3
1

Research data for: Krishna de Guzman, M., Stanić-Vučinić, D., Gligorijević, N., Wimmer, L., Gasparyan, M., Lujić, T., Vasović, T., Dailey, L. A., Van Haute, S.,& Ćirković-Veličković, T.. (2023). Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion. in Environmental Pollution Elsevier., 335, 122282. https://doi.org/10.1016/j.envpol.2023.122282

de Guzman, Maria Krishna; Stanić-Vučinić, Dragana; Gligorijević, Nikola; Wimmer, Lukas; Gasparyan, Manvel; Lujić, Tamara; Vasović, Tamara; Dailey, Lea Ann; Van Haute, Sam; Ćirković-Veličković, Tanja

(2023)

TY  - DATA
AU  - de Guzman, Maria Krishna
AU  - Stanić-Vučinić, Dragana
AU  - Gligorijević, Nikola
AU  - Wimmer, Lukas
AU  - Gasparyan, Manvel
AU  - Lujić, Tamara
AU  - Vasović, Tamara
AU  - Dailey, Lea Ann
AU  - Van Haute, Sam
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6463
AB  - The analysis of structural changes of pepsin in the presence of polystyrene microplastic of 10 and 100 µm in size in simulated gastric fluid. Data obtained from J-815 CD spectropolarimeter, and further analyzed by Origin and Excel software.
T2  - Environmental Pollution
T1  - Research data for: Krishna de Guzman, M., Stanić-Vučinić, D., Gligorijević, N., Wimmer, L., Gasparyan, M., Lujić, T., Vasović, T., Dailey, L. A., Van Haute, S.,& Ćirković-Veličković, T.. (2023). Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion. in Environmental Pollution Elsevier., 335, 122282. https://doi.org/10.1016/j.envpol.2023.122282
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6463
ER  - 
@misc{
author = "de Guzman, Maria Krishna and Stanić-Vučinić, Dragana and Gligorijević, Nikola and Wimmer, Lukas and Gasparyan, Manvel and Lujić, Tamara and Vasović, Tamara and Dailey, Lea Ann and Van Haute, Sam and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "The analysis of structural changes of pepsin in the presence of polystyrene microplastic of 10 and 100 µm in size in simulated gastric fluid. Data obtained from J-815 CD spectropolarimeter, and further analyzed by Origin and Excel software.",
journal = "Environmental Pollution",
title = "Research data for: Krishna de Guzman, M., Stanić-Vučinić, D., Gligorijević, N., Wimmer, L., Gasparyan, M., Lujić, T., Vasović, T., Dailey, L. A., Van Haute, S.,& Ćirković-Veličković, T.. (2023). Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion. in Environmental Pollution Elsevier., 335, 122282. https://doi.org/10.1016/j.envpol.2023.122282",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6463"
}
de Guzman, M. K., Stanić-Vučinić, D., Gligorijević, N., Wimmer, L., Gasparyan, M., Lujić, T., Vasović, T., Dailey, L. A., Van Haute, S.,& Ćirković-Veličković, T.. (2023). Research data for: Krishna de Guzman, M., Stanić-Vučinić, D., Gligorijević, N., Wimmer, L., Gasparyan, M., Lujić, T., Vasović, T., Dailey, L. A., Van Haute, S.,& Ćirković-Veličković, T.. (2023). Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion. in Environmental Pollution Elsevier., 335, 122282. https://doi.org/10.1016/j.envpol.2023.122282. in Environmental Pollution.
https://hdl.handle.net/21.15107/rcub_cherry_6463
de Guzman MK, Stanić-Vučinić D, Gligorijević N, Wimmer L, Gasparyan M, Lujić T, Vasović T, Dailey LA, Van Haute S, Ćirković-Veličković T. Research data for: Krishna de Guzman, M., Stanić-Vučinić, D., Gligorijević, N., Wimmer, L., Gasparyan, M., Lujić, T., Vasović, T., Dailey, L. A., Van Haute, S.,& Ćirković-Veličković, T.. (2023). Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion. in Environmental Pollution Elsevier., 335, 122282. https://doi.org/10.1016/j.envpol.2023.122282. in Environmental Pollution. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6463 .
de Guzman, Maria Krishna, Stanić-Vučinić, Dragana, Gligorijević, Nikola, Wimmer, Lukas, Gasparyan, Manvel, Lujić, Tamara, Vasović, Tamara, Dailey, Lea Ann, Van Haute, Sam, Ćirković-Veličković, Tanja, "Research data for: Krishna de Guzman, M., Stanić-Vučinić, D., Gligorijević, N., Wimmer, L., Gasparyan, M., Lujić, T., Vasović, T., Dailey, L. A., Van Haute, S.,& Ćirković-Veličković, T.. (2023). Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion. in Environmental Pollution Elsevier., 335, 122282. https://doi.org/10.1016/j.envpol.2023.122282" in Environmental Pollution (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6463 .

Research data no. 2 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703

Đapović, Milica; Apostolović, Danijela; Poštić, Vojislava; Lujić, Tamara; Jovanović, Vesna; Stanić-Vučinić, Dragana; van Hage, Marianne; Maslak, Veselin; Ćirković-Veličković, Tanja

(MDPI, 2023)

TY  - DATA
AU  - Đapović, Milica
AU  - Apostolović, Danijela
AU  - Poštić, Vojislava
AU  - Lujić, Tamara
AU  - Jovanović, Vesna
AU  - Stanić-Vučinić, Dragana
AU  - van Hage, Marianne
AU  - Maslak, Veselin
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6464
AB  - Determination of hydrodynamic diameter of PET NPs washed and dispersed in water, BSA (0.05%), and SDS (0.1%), and NPs unwashed and dispersed in water.  Zeta potential, mobility, and conductivity determination in PET NPs at different stages of purification. Output from the Malvern zetasizer Nano-ZS ZEN 3600 (Malvern Panalytical, Malvern, UK).
PB  - MDPI
T2  - Polymers
T1  - Research data no. 2 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6464
ER  - 
@misc{
author = "Đapović, Milica and Apostolović, Danijela and Poštić, Vojislava and Lujić, Tamara and Jovanović, Vesna and Stanić-Vučinić, Dragana and van Hage, Marianne and Maslak, Veselin and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "Determination of hydrodynamic diameter of PET NPs washed and dispersed in water, BSA (0.05%), and SDS (0.1%), and NPs unwashed and dispersed in water.  Zeta potential, mobility, and conductivity determination in PET NPs at different stages of purification. Output from the Malvern zetasizer Nano-ZS ZEN 3600 (Malvern Panalytical, Malvern, UK).",
publisher = "MDPI",
journal = "Polymers",
title = "Research data no. 2 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6464"
}
Đapović, M., Apostolović, D., Poštić, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Research data no. 2 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703. in Polymers
MDPI..
https://hdl.handle.net/21.15107/rcub_cherry_6464
Đapović M, Apostolović D, Poštić V, Lujić T, Jovanović V, Stanić-Vučinić D, van Hage M, Maslak V, Ćirković-Veličković T. Research data no. 2 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703. in Polymers. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6464 .
Đapović, Milica, Apostolović, Danijela, Poštić, Vojislava, Lujić, Tamara, Jovanović, Vesna, Stanić-Vučinić, Dragana, van Hage, Marianne, Maslak, Veselin, Ćirković-Veličković, Tanja, "Research data no. 2 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703" in Polymers (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6464 .

Research data no. 3 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703

Đapović, Milica; Apostolović, Danijela; Poštić, Vojislava; Lujić, Tamara; Jovanović, Vesna; Stanić-Vučinić, Dragana; van Hage, Marianne; Maslak, Veselin; Ćirković-Veličković, Tanja

(MDPI, 2023)

TY  - DATA
AU  - Đapović, Milica
AU  - Apostolović, Danijela
AU  - Poštić, Vojislava
AU  - Lujić, Tamara
AU  - Jovanović, Vesna
AU  - Stanić-Vučinić, Dragana
AU  - van Hage, Marianne
AU  - Maslak, Veselin
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6467
AB  - The presence of the ionic surfactant sodium dodecyl sulfate (SDS) was characterized by 1H NMR, where the relative ratio of NP/surfactant was monitored on the basis of the chemical shifts characteristic of PET and SDS. 1H NMR spectra of the NPs preparation before and during all the washing steps. Determination of SDS Level in Corona of PET NPs by 1H NMR. Output from the Varian/Agilent NMR 400 MHz. NMR spectra were processed in Mnova software.
PB  - MDPI
T2  - Polymers
T1  - Research data no. 3 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6467
ER  - 
@misc{
author = "Đapović, Milica and Apostolović, Danijela and Poštić, Vojislava and Lujić, Tamara and Jovanović, Vesna and Stanić-Vučinić, Dragana and van Hage, Marianne and Maslak, Veselin and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "The presence of the ionic surfactant sodium dodecyl sulfate (SDS) was characterized by 1H NMR, where the relative ratio of NP/surfactant was monitored on the basis of the chemical shifts characteristic of PET and SDS. 1H NMR spectra of the NPs preparation before and during all the washing steps. Determination of SDS Level in Corona of PET NPs by 1H NMR. Output from the Varian/Agilent NMR 400 MHz. NMR spectra were processed in Mnova software.",
publisher = "MDPI",
journal = "Polymers",
title = "Research data no. 3 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6467"
}
Đapović, M., Apostolović, D., Poštić, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Research data no. 3 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703. in Polymers
MDPI..
https://hdl.handle.net/21.15107/rcub_cherry_6467
Đapović M, Apostolović D, Poštić V, Lujić T, Jovanović V, Stanić-Vučinić D, van Hage M, Maslak V, Ćirković-Veličković T. Research data no. 3 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703. in Polymers. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6467 .
Đapović, Milica, Apostolović, Danijela, Poštić, Vojislava, Lujić, Tamara, Jovanović, Vesna, Stanić-Vučinić, Dragana, van Hage, Marianne, Maslak, Veselin, Ćirković-Veličković, Tanja, "Research data no. 3 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703" in Polymers (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6467 .

Research data no. 4 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703

Đapović, Milica; Apostolović, Danijela; Poštić, Vojislava; Lujić, Tamara; Jovanović, Vesna; Stanić-Vučinić, Dragana; van Hage, Marianne; Maslak, Veselin; Ćirković-Veličković, Tanja

(MDPI, 2023)

TY  - DATA
AU  - Đapović, Milica
AU  - Apostolović, Danijela
AU  - Poštić, Vojislava
AU  - Lujić, Tamara
AU  - Jovanović, Vesna
AU  - Stanić-Vučinić, Dragana
AU  - van Hage, Marianne
AU  - Maslak, Veselin
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6468
AB  - Size distributions (by intensity percentage) of NPs washed suspended in different dispersants, water,BSA (0.05%),SDS (0.1 %). Distributions data from different combined fractions: from NP-10 to NP-30; from NP-40 to NP-60 and from NP-70 to NP-90. Output from the Malvern zetasizer Nano-ZS ZEN 3600 (Malvern Panalytical, Malvern, UK).
PB  - MDPI
T2  - Polymers
T1  - Research data no. 4 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6468
ER  - 
@misc{
author = "Đapović, Milica and Apostolović, Danijela and Poštić, Vojislava and Lujić, Tamara and Jovanović, Vesna and Stanić-Vučinić, Dragana and van Hage, Marianne and Maslak, Veselin and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "Size distributions (by intensity percentage) of NPs washed suspended in different dispersants, water,BSA (0.05%),SDS (0.1 %). Distributions data from different combined fractions: from NP-10 to NP-30; from NP-40 to NP-60 and from NP-70 to NP-90. Output from the Malvern zetasizer Nano-ZS ZEN 3600 (Malvern Panalytical, Malvern, UK).",
publisher = "MDPI",
journal = "Polymers",
title = "Research data no. 4 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6468"
}
Đapović, M., Apostolović, D., Poštić, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Research data no. 4 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703. in Polymers
MDPI..
https://hdl.handle.net/21.15107/rcub_cherry_6468
Đapović M, Apostolović D, Poštić V, Lujić T, Jovanović V, Stanić-Vučinić D, van Hage M, Maslak V, Ćirković-Veličković T. Research data no. 4 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703. in Polymers. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6468 .
Đapović, Milica, Apostolović, Danijela, Poštić, Vojislava, Lujić, Tamara, Jovanović, Vesna, Stanić-Vučinić, Dragana, van Hage, Marianne, Maslak, Veselin, Ćirković-Veličković, Tanja, "Research data no. 4 for: Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers MDPI., 15(24), 4703. https://doi.org/10.3390/polym15244703" in Polymers (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6468 .

Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes

Djapovic, Milica; Apostolović, Danijela; Postic, Vojislava; Lujić, Tamara; Jovanović, Vesna; Stanić-Vučinić, Dragana; van Hage, Marianne; Maslak, Veselin; Ćirković-Veličković, Tanja

(MDPI, 2023)

TY  - JOUR
AU  - Djapovic, Milica
AU  - Apostolović, Danijela
AU  - Postic, Vojislava
AU  - Lujić, Tamara
AU  - Jovanović, Vesna
AU  - Stanić-Vučinić, Dragana
AU  - van Hage, Marianne
AU  - Maslak, Veselin
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6433
AB  - Manufactured nanoplastic particles (NPs) are indispensable for in vitro and in vivo testing and a health risk assessment of this emerging environmental contaminant is needed. The high surface area and inherent hydrophobicity of plastic materials makes the production of NPs devoid of any contaminants very challenging. In this study, we produced nanoprecipitated polyethylene terephthalate (PET) NPs (300 nm hydrodynamic diameter) with an overall yield of 0.76%. The presence of the ionic surfactant sodium dodecyl sulfate (SDS) was characterized by 1H NMR, where the relative ratio of NP/surfactant was monitored on the basis of the chemical shifts characteristic of PET and SDS. For a wide range of surfactant/NP ratios (17:100 to 1.2:100), the measured zeta potential changed from −42.10 to −34.93 mV, but with an NP concentration up to 100 μg/mL, no clear differences were observed in the cellular assays performed in protein-rich media on primary human cells. The remaining impurities contributed to the outcome of the biological assays applied in protein-free buffers, such as human red blood cell hemolysis. The presence of SDS increased the NP-induced hemolysis by 1.5% in protein-rich buffer and by 7.5% in protein-free buffer. As the size, shape, zeta potential, and contaminants of NPs may all be relevant parameters for the biological effects of NPs, the relative quantification of impurities exemplified in our work by the application of 1H NMR for PET NPs and the ionic surfactant SDS could be a valuable auxiliary method in the quality control of manufactured NPs.
PB  - MDPI
T2  - Polymers
T1  - Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes
VL  - 15
IS  - 24
SP  - 4703
DO  - 10.3390/polym15244703
ER  - 
@article{
author = "Djapovic, Milica and Apostolović, Danijela and Postic, Vojislava and Lujić, Tamara and Jovanović, Vesna and Stanić-Vučinić, Dragana and van Hage, Marianne and Maslak, Veselin and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "Manufactured nanoplastic particles (NPs) are indispensable for in vitro and in vivo testing and a health risk assessment of this emerging environmental contaminant is needed. The high surface area and inherent hydrophobicity of plastic materials makes the production of NPs devoid of any contaminants very challenging. In this study, we produced nanoprecipitated polyethylene terephthalate (PET) NPs (300 nm hydrodynamic diameter) with an overall yield of 0.76%. The presence of the ionic surfactant sodium dodecyl sulfate (SDS) was characterized by 1H NMR, where the relative ratio of NP/surfactant was monitored on the basis of the chemical shifts characteristic of PET and SDS. For a wide range of surfactant/NP ratios (17:100 to 1.2:100), the measured zeta potential changed from −42.10 to −34.93 mV, but with an NP concentration up to 100 μg/mL, no clear differences were observed in the cellular assays performed in protein-rich media on primary human cells. The remaining impurities contributed to the outcome of the biological assays applied in protein-free buffers, such as human red blood cell hemolysis. The presence of SDS increased the NP-induced hemolysis by 1.5% in protein-rich buffer and by 7.5% in protein-free buffer. As the size, shape, zeta potential, and contaminants of NPs may all be relevant parameters for the biological effects of NPs, the relative quantification of impurities exemplified in our work by the application of 1H NMR for PET NPs and the ionic surfactant SDS could be a valuable auxiliary method in the quality control of manufactured NPs.",
publisher = "MDPI",
journal = "Polymers",
title = "Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes",
volume = "15",
number = "24",
pages = "4703",
doi = "10.3390/polym15244703"
}
Djapovic, M., Apostolović, D., Postic, V., Lujić, T., Jovanović, V., Stanić-Vučinić, D., van Hage, M., Maslak, V.,& Ćirković-Veličković, T.. (2023). Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers
MDPI., 15(24), 4703.
https://doi.org/10.3390/polym15244703
Djapovic M, Apostolović D, Postic V, Lujić T, Jovanović V, Stanić-Vučinić D, van Hage M, Maslak V, Ćirković-Veličković T. Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes. in Polymers. 2023;15(24):4703.
doi:10.3390/polym15244703 .
Djapovic, Milica, Apostolović, Danijela, Postic, Vojislava, Lujić, Tamara, Jovanović, Vesna, Stanić-Vučinić, Dragana, van Hage, Marianne, Maslak, Veselin, Ćirković-Veličković, Tanja, "Characterization of Nanoprecipitated PET Nanoplastics by 1H NMR and Impact of Residual Ionic Surfactant on Viability of Human Primary Mononuclear Cells and Hemolysis of Erythrocytes" in Polymers, 15, no. 24 (2023):4703,
https://doi.org/10.3390/polym15244703 . .
1

The effect of food processing and packaging of clams on the content of tropomyosin

Jovanović, Vesna; Radomirović, Mirjana Ž.; Krstić-Ristivojević, Maja; Stanić-Vučinić, Dragana; Ćirković-Veličković, Tanja

(Beograd : Srpsko hemijsko društvo, 2023)

TY  - CONF
AU  - Jovanović, Vesna
AU  - Radomirović, Mirjana Ž.
AU  - Krstić-Ristivojević, Maja
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6023
AB  - In the last several decades, the trend of seafood consumption has significantly increased not only in the countries with a tradition of seafood consumption, but also in other ones [1]. The increase in the world's population and the awareness of healthy food, the globalization of markets, and the development of aquaculture are some of the factors that have led to this trend. The aquaculture of shellfish like clams, mussels, oysters and scallops has been very developed all around the world and the food products based on them have become part of the daily diet for many consumers. In addition, these food products are considered healthy food because of the high content of proteins and essential fatty acids, but their consumption may carry some risks of food allergy. Tropomyosin from shellfish (TPM) is the major allergen responsible for the development of anaphylaxis in persons with food allergy. The content of TPM in shellfish and its bioavailability from food products can have potential influence on the sensitization of consumers to TPM. It is known that food processing can change the bioavailability of food allergens [2]. The main goal of this study was the investigation of how processing and packaging of shellfish samples can affect the content of TPM in them.
For this study, clam Venerupis philippinarum was chosen as the species with the highest world aquaculture production [1]. After the purchasing of live clams, the animals were separated into 5 groups for the next treatments: fresh live (control group), freshly removed inner content was kept at +4°C for 3 days (three days` shelf-life), frozen in a plastic bag and kept at -20 °C during 7 days, marinated and kept in a glass jar at room temperature during 8 days and freshly boiled. After processing and packaging of samples, the total protein extracts were prepared in 10 mM sodium phosphate buffer pH 7.4 1M NaCl, 1 mM PMSF and the concentration of total proteins was determined by BCA method. The concentration of TPM in the total protein extracts was determined using a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) using in-house prepared clams` TPM standard. The content of TPM (μg) in the samples was expressed per mg of extracted soluble proteins, individual animal and grams of soft wet tissue.
The cooked samples have significantly higher TPM content expressed per gram of soft wet tissue compared to all other treatments. Food processing such as freezing, marinating, or extending the shelf-life at 4°C by 3 days has very little effect on the change in TPM content per gram of soft wet tissue compared to the fresh samples. The processing of clams, like cooking or marinating, caused the content of total soluble extracted proteins to be three to four times lower compared to the other three treatments. In these samples the obtained ratio of the total TPM/ total soluble extracted proteins ratio was the highest. This result can be explained by the fact that TPM is thermostable and stays soluble after cooking, while other proteins become insoluble because of denaturation. The lower ratio of TPM/ total soluble extracted proteins was found in marinated samples compared to the cooked samples. The lowest total tropomyosin/ total soluble extracted proteins ratio was found in 3 days’ shelf-life. Treatments like cooking, marinating and keeping the inner content of the shell at +4°C can significantly affect extractability of proteins, particularly affecting the ratio of major allergen TPM in the total protein extracts. Further studies are needed to examine bioaccessibility of TPM in different biologically relevant fluids (gastric/intestinal) and during digestion in relation to the processing conditions.
PB  - Beograd : Srpsko hemijsko društvo
C3  - XXII EuroFoodChem conference, Book of Abstracts, 14th-16th June, 2023.
T1  - The effect of food processing and packaging of clams on the content of tropomyosin
SP  - 240
EP  - 240
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6023
ER  - 
@conference{
author = "Jovanović, Vesna and Radomirović, Mirjana Ž. and Krstić-Ristivojević, Maja and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "In the last several decades, the trend of seafood consumption has significantly increased not only in the countries with a tradition of seafood consumption, but also in other ones [1]. The increase in the world's population and the awareness of healthy food, the globalization of markets, and the development of aquaculture are some of the factors that have led to this trend. The aquaculture of shellfish like clams, mussels, oysters and scallops has been very developed all around the world and the food products based on them have become part of the daily diet for many consumers. In addition, these food products are considered healthy food because of the high content of proteins and essential fatty acids, but their consumption may carry some risks of food allergy. Tropomyosin from shellfish (TPM) is the major allergen responsible for the development of anaphylaxis in persons with food allergy. The content of TPM in shellfish and its bioavailability from food products can have potential influence on the sensitization of consumers to TPM. It is known that food processing can change the bioavailability of food allergens [2]. The main goal of this study was the investigation of how processing and packaging of shellfish samples can affect the content of TPM in them.
For this study, clam Venerupis philippinarum was chosen as the species with the highest world aquaculture production [1]. After the purchasing of live clams, the animals were separated into 5 groups for the next treatments: fresh live (control group), freshly removed inner content was kept at +4°C for 3 days (three days` shelf-life), frozen in a plastic bag and kept at -20 °C during 7 days, marinated and kept in a glass jar at room temperature during 8 days and freshly boiled. After processing and packaging of samples, the total protein extracts were prepared in 10 mM sodium phosphate buffer pH 7.4 1M NaCl, 1 mM PMSF and the concentration of total proteins was determined by BCA method. The concentration of TPM in the total protein extracts was determined using a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) using in-house prepared clams` TPM standard. The content of TPM (μg) in the samples was expressed per mg of extracted soluble proteins, individual animal and grams of soft wet tissue.
The cooked samples have significantly higher TPM content expressed per gram of soft wet tissue compared to all other treatments. Food processing such as freezing, marinating, or extending the shelf-life at 4°C by 3 days has very little effect on the change in TPM content per gram of soft wet tissue compared to the fresh samples. The processing of clams, like cooking or marinating, caused the content of total soluble extracted proteins to be three to four times lower compared to the other three treatments. In these samples the obtained ratio of the total TPM/ total soluble extracted proteins ratio was the highest. This result can be explained by the fact that TPM is thermostable and stays soluble after cooking, while other proteins become insoluble because of denaturation. The lower ratio of TPM/ total soluble extracted proteins was found in marinated samples compared to the cooked samples. The lowest total tropomyosin/ total soluble extracted proteins ratio was found in 3 days’ shelf-life. Treatments like cooking, marinating and keeping the inner content of the shell at +4°C can significantly affect extractability of proteins, particularly affecting the ratio of major allergen TPM in the total protein extracts. Further studies are needed to examine bioaccessibility of TPM in different biologically relevant fluids (gastric/intestinal) and during digestion in relation to the processing conditions.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "XXII EuroFoodChem conference, Book of Abstracts, 14th-16th June, 2023.",
title = "The effect of food processing and packaging of clams on the content of tropomyosin",
pages = "240-240",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6023"
}
Jovanović, V., Radomirović, M. Ž., Krstić-Ristivojević, M., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2023). The effect of food processing and packaging of clams on the content of tropomyosin. in XXII EuroFoodChem conference, Book of Abstracts, 14th-16th June, 2023.
Beograd : Srpsko hemijsko društvo., 240-240.
https://hdl.handle.net/21.15107/rcub_cherry_6023
Jovanović V, Radomirović MŽ, Krstić-Ristivojević M, Stanić-Vučinić D, Ćirković-Veličković T. The effect of food processing and packaging of clams on the content of tropomyosin. in XXII EuroFoodChem conference, Book of Abstracts, 14th-16th June, 2023.. 2023;:240-240.
https://hdl.handle.net/21.15107/rcub_cherry_6023 .
Jovanović, Vesna, Radomirović, Mirjana Ž., Krstić-Ristivojević, Maja, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "The effect of food processing and packaging of clams on the content of tropomyosin" in XXII EuroFoodChem conference, Book of Abstracts, 14th-16th June, 2023. (2023):240-240,
https://hdl.handle.net/21.15107/rcub_cherry_6023 .

Tropomyosin quantification in seafood samples-right choice of standard makes a difference

Krstić-Ristivojević, Maja; Jovanović, Vesna; Radomirović, Mirjana Ž.; Trifunović, Olga; Stanić-Vučinić, Dragana; Ćirković-Veličković, Tanja

(Beograd : Srpsko hemijsko društvo, 2023)

TY  - CONF
AU  - Krstić-Ristivojević, Maja
AU  - Jovanović, Vesna
AU  - Radomirović, Mirjana Ž.
AU  - Trifunović, Olga
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6024
AB  - In the last 50 years, the annual per capita consumption of seafood products worldwide has more than doubled, from almost 10 kg in 1960 to over 20 kg in 2014. Seafood protein is an essential part of the diet in many countries, particularly where total protein intake is low [1]. However, as defined by the European Community, fish, and shellfish tropomyosins (TPM) are major allergens and major causes of anaphylaxis [2]. The increasing prevalence of food allergies is consistent with the increasing pollution of soil and water with plastic particles. To investigate the potential link between increasing plastic pollution and increasing food allergy prevalence, we aim to develop methods for precise and accurate monitoring of allergens and plastic in real seafood samples.
TPM was isolated from shrimp (Litopenaeus vannamei), clams (Venerupis philippinarum), and mussels (Mytilus galloprovincialis). The obtained in-house TPM proteins from three different sources were resolved using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The concentration of TPM in seashell samples from different geographical origin was determined using a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with prior optimization of adequate TPM standard curve using commercial and non-commercial in-house prepared TPM standards.
TPM standards resolved via 2D-PAGE revealed the presence of two isoforms of shrimp and mussels TPM standard, one dominant and one less abundant isoform. Two isoforms from both seafood sources, shrimp and mussels, are slightly different in molecular weight and pI value. As for the TPM standard obtained from clams, the 2D electrophoregram showed possibly eight isoforms with small differences in mass and pI values. Furthermore, the presence of three dominant isoforms can be observed that differ slightly in molecular mass, while other isoforms also differ in pI value. The ELISA results, regarding TPM standard curve optimization, showed that in both the commercial shrimp TPM and in-house shrimp TPM standards, sigmoidal concentration dependence is present in a range of 50 to 0.05 ng/ml, using serial double dilutions. On the other hand, TPM standards isolated from mussels and clams show sigmoidal concentration dependence in the range of 45 to 0.044 μg/ml with using the identical combination of capture and detection antibodies and serial double dilutions. TPM concentrations in clams and mussel samples extrapolated from standard curves of commercial shrimp TPM standard and corresponding in-house TPM standards are presented in Table 1.
Differences in TPM concentration of the same sample using different TPM standards differ from 40 to 600 times, which strongly indicates that the right choice of TPM standard is a critical step for accurate and precise determination of TPM concentration in seafood samples.
PB  - Beograd : Srpsko hemijsko društvo
C3  - XXII EuroFoodChem conference, 14th-16th June, 2023. In: Book of Abstracts
T1  - Tropomyosin quantification in seafood samples-right choice of standard makes a difference
SP  - 132
EP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6024
ER  - 
@conference{
author = "Krstić-Ristivojević, Maja and Jovanović, Vesna and Radomirović, Mirjana Ž. and Trifunović, Olga and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "In the last 50 years, the annual per capita consumption of seafood products worldwide has more than doubled, from almost 10 kg in 1960 to over 20 kg in 2014. Seafood protein is an essential part of the diet in many countries, particularly where total protein intake is low [1]. However, as defined by the European Community, fish, and shellfish tropomyosins (TPM) are major allergens and major causes of anaphylaxis [2]. The increasing prevalence of food allergies is consistent with the increasing pollution of soil and water with plastic particles. To investigate the potential link between increasing plastic pollution and increasing food allergy prevalence, we aim to develop methods for precise and accurate monitoring of allergens and plastic in real seafood samples.
TPM was isolated from shrimp (Litopenaeus vannamei), clams (Venerupis philippinarum), and mussels (Mytilus galloprovincialis). The obtained in-house TPM proteins from three different sources were resolved using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The concentration of TPM in seashell samples from different geographical origin was determined using a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with prior optimization of adequate TPM standard curve using commercial and non-commercial in-house prepared TPM standards.
TPM standards resolved via 2D-PAGE revealed the presence of two isoforms of shrimp and mussels TPM standard, one dominant and one less abundant isoform. Two isoforms from both seafood sources, shrimp and mussels, are slightly different in molecular weight and pI value. As for the TPM standard obtained from clams, the 2D electrophoregram showed possibly eight isoforms with small differences in mass and pI values. Furthermore, the presence of three dominant isoforms can be observed that differ slightly in molecular mass, while other isoforms also differ in pI value. The ELISA results, regarding TPM standard curve optimization, showed that in both the commercial shrimp TPM and in-house shrimp TPM standards, sigmoidal concentration dependence is present in a range of 50 to 0.05 ng/ml, using serial double dilutions. On the other hand, TPM standards isolated from mussels and clams show sigmoidal concentration dependence in the range of 45 to 0.044 μg/ml with using the identical combination of capture and detection antibodies and serial double dilutions. TPM concentrations in clams and mussel samples extrapolated from standard curves of commercial shrimp TPM standard and corresponding in-house TPM standards are presented in Table 1.
Differences in TPM concentration of the same sample using different TPM standards differ from 40 to 600 times, which strongly indicates that the right choice of TPM standard is a critical step for accurate and precise determination of TPM concentration in seafood samples.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "XXII EuroFoodChem conference, 14th-16th June, 2023. In: Book of Abstracts",
title = "Tropomyosin quantification in seafood samples-right choice of standard makes a difference",
pages = "132-132",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6024"
}
Krstić-Ristivojević, M., Jovanović, V., Radomirović, M. Ž., Trifunović, O., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2023). Tropomyosin quantification in seafood samples-right choice of standard makes a difference. in XXII EuroFoodChem conference, 14th-16th June, 2023. In: Book of Abstracts
Beograd : Srpsko hemijsko društvo., 132-132.
https://hdl.handle.net/21.15107/rcub_cherry_6024
Krstić-Ristivojević M, Jovanović V, Radomirović MŽ, Trifunović O, Stanić-Vučinić D, Ćirković-Veličković T. Tropomyosin quantification in seafood samples-right choice of standard makes a difference. in XXII EuroFoodChem conference, 14th-16th June, 2023. In: Book of Abstracts. 2023;:132-132.
https://hdl.handle.net/21.15107/rcub_cherry_6024 .
Krstić-Ristivojević, Maja, Jovanović, Vesna, Radomirović, Mirjana Ž., Trifunović, Olga, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Tropomyosin quantification in seafood samples-right choice of standard makes a difference" in XXII EuroFoodChem conference, 14th-16th June, 2023. In: Book of Abstracts (2023):132-132,
https://hdl.handle.net/21.15107/rcub_cherry_6024 .

Exploring and strengthening the potential of R-phycocyanin from Nori flakes as a food colourant

Veličković, Luka; Simović, Ana; Gligorijević, Nikola; Thureau, Aurélien; Obradović, Milica; Vasović, Tamara; Sotiroudis, Georgios; Zoumpanioti, Maria; Brûlet, Annie; Ćirković-Veličković, Tanja; Combet, Sophie; Nikolić, Milan; Minić, Simeon

(Elsevier, 2023)

TY  - JOUR
AU  - Veličković, Luka
AU  - Simović, Ana
AU  - Gligorijević, Nikola
AU  - Thureau, Aurélien
AU  - Obradović, Milica
AU  - Vasović, Tamara
AU  - Sotiroudis, Georgios
AU  - Zoumpanioti, Maria
AU  - Brûlet, Annie
AU  - Ćirković-Veličković, Tanja
AU  - Combet, Sophie
AU  - Nikolić, Milan
AU  - Minić, Simeon
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6273
AB  - This study aimed to purify, characterise and stabilise the natural food colourant, R-phycocyanin (R-PC), from the red algae Porphyra spp. (Nori). We purified R-PC from dried Nori flakes with a high purity ratio (A618/A280 ≥ 3.4) in native form (α-helix content 53%). SAXS measurements revealed that R-PC is trimeric ((αβ)3) in solution. The thermal denaturation of α-helix revealed one transition (Tm at 52 °C), while the pH stability study showed R-PC is stable in the pH range 4–8. The thermal treatment of R-PC at 60 °C has detrimental and irreversible effects on R-PC colour and antioxidant capacity (22 % of residual capacity). However, immobilisation of R-PC within calcium alginate beads completely preserves R-PC colour and mainly retains its antioxidant ability (78 % of residual capacity). Results give new insights into the stability of R-PC and preservation of its purple colour and bioactivity by encapsulation in calcium alginate beads.
PB  - Elsevier
T2  - Food Chemistry
T1  - Exploring and strengthening the potential of R-phycocyanin from Nori flakes as a food colourant
VL  - 426
SP  - 136669
DO  - 10.1016/j.foodchem.2023.136669
ER  - 
@article{
author = "Veličković, Luka and Simović, Ana and Gligorijević, Nikola and Thureau, Aurélien and Obradović, Milica and Vasović, Tamara and Sotiroudis, Georgios and Zoumpanioti, Maria and Brûlet, Annie and Ćirković-Veličković, Tanja and Combet, Sophie and Nikolić, Milan and Minić, Simeon",
year = "2023",
abstract = "This study aimed to purify, characterise and stabilise the natural food colourant, R-phycocyanin (R-PC), from the red algae Porphyra spp. (Nori). We purified R-PC from dried Nori flakes with a high purity ratio (A618/A280 ≥ 3.4) in native form (α-helix content 53%). SAXS measurements revealed that R-PC is trimeric ((αβ)3) in solution. The thermal denaturation of α-helix revealed one transition (Tm at 52 °C), while the pH stability study showed R-PC is stable in the pH range 4–8. The thermal treatment of R-PC at 60 °C has detrimental and irreversible effects on R-PC colour and antioxidant capacity (22 % of residual capacity). However, immobilisation of R-PC within calcium alginate beads completely preserves R-PC colour and mainly retains its antioxidant ability (78 % of residual capacity). Results give new insights into the stability of R-PC and preservation of its purple colour and bioactivity by encapsulation in calcium alginate beads.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Exploring and strengthening the potential of R-phycocyanin from Nori flakes as a food colourant",
volume = "426",
pages = "136669",
doi = "10.1016/j.foodchem.2023.136669"
}
Veličković, L., Simović, A., Gligorijević, N., Thureau, A., Obradović, M., Vasović, T., Sotiroudis, G., Zoumpanioti, M., Brûlet, A., Ćirković-Veličković, T., Combet, S., Nikolić, M.,& Minić, S.. (2023). Exploring and strengthening the potential of R-phycocyanin from Nori flakes as a food colourant. in Food Chemistry
Elsevier., 426, 136669.
https://doi.org/10.1016/j.foodchem.2023.136669
Veličković L, Simović A, Gligorijević N, Thureau A, Obradović M, Vasović T, Sotiroudis G, Zoumpanioti M, Brûlet A, Ćirković-Veličković T, Combet S, Nikolić M, Minić S. Exploring and strengthening the potential of R-phycocyanin from Nori flakes as a food colourant. in Food Chemistry. 2023;426:136669.
doi:10.1016/j.foodchem.2023.136669 .
Veličković, Luka, Simović, Ana, Gligorijević, Nikola, Thureau, Aurélien, Obradović, Milica, Vasović, Tamara, Sotiroudis, Georgios, Zoumpanioti, Maria, Brûlet, Annie, Ćirković-Veličković, Tanja, Combet, Sophie, Nikolić, Milan, Minić, Simeon, "Exploring and strengthening the potential of R-phycocyanin from Nori flakes as a food colourant" in Food Chemistry, 426 (2023):136669,
https://doi.org/10.1016/j.foodchem.2023.136669 . .
2
2

Proteomic insight into allergenic food corona on polyethylene terephthalate microplastics

Lujić, Tamara; Gligorijević, Nikola; Jovanović, Vesna; Aćimović, Jelena; Mitić, Dragana; Vasović, Tamara; Stojadinović, Marija; Stanić-Vučinić, Dragana; Ćirković-Veličković, Tanja

(Italian Proteomics Association, 2023)

TY  - CONF
AU  - Lujić, Tamara
AU  - Gligorijević, Nikola
AU  - Jovanović, Vesna
AU  - Aćimović, Jelena
AU  - Mitić, Dragana
AU  - Vasović, Tamara
AU  - Stojadinović, Marija
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6424
AB  - Microplastics is abundant in the environment, food and beverages and get ingested by humans. Its complex interplay with proteins lead to formation of corona. Tightly bound proteins represent hard corona, while weaker binding partners are found in soft corona. Separation of hard and soft corona of allergenic proteins of shrimps, eggs and cow’s milk, tropomyosin (TPM), ovalbumin (OVA) and beta-lactoglobulin (BLG) and identification of binding partners by proteomics was aim of our study.
Allergenic proteins were purified from egg white, shrimps and cow’s milk. Binding to polyethylene terephthalate microplastics (PET) (70-100 m) was probed at pH 7 for purified allergens and egg white proteins. After establishment of binding equilibrium, soft and hard corona were separated and analyzed by SDS PAGE, followed by identification of bound proteins by nanoLC-HRMS. Binding of all allergenic proteins was observed in both soft and hard corona. Soft corona contains exclusively intact, full length OVA, TPM and BLG. Hard corona is enriched for truncated OVA and oligomers of TPM. OVA fragments are partially or fully enfolded and have higher level of exposed hydrophobic patches resulting in higher affinity for PET microplastics. In comparison to OVA and TPM, hard corona of BLG is less abundant under similar conditions. BLG is compact globular protein with lower level of exposed hydrophobic patches in comparison to ovalbumin and tropomyosin. In hard corona, trace amounts of contaminating alfa-lactalbumin become enriched. In the presence of egg white protein extract OVA forms both SC and HC on microplastics, being the dominant protein of hard corona (with ovotransferrin). Lysozyme and ovomucin are present only in hard corona. Both proteins are known for their strong bioactivity and represent a small fraction of total egg white proteins.
Our results show that allergenic proteins form hard corona on PET microplastics. Among egg white proteins, minor proteins such as lysozyme and ovomucin become enriched. Denaturing effect of strong binding to microplastics may change functional characteristics of allergens and bioactive proteins of foods and should be further investigated in functional assays.
Acknowledgment: This study was supported by IMPTOX European Union's Horizon 2020 research and innovation program (grant number 965173).
PB  - Italian Proteomics Association
C3  - ItPA HPS and SePA XVII International Congress: Proteomics and Metabolomics towards Global Health, November 29th – December 1st, 2023, Roma, Italy
T1  - Proteomic insight into allergenic food corona on polyethylene terephthalate microplastics
SP  - 11
EP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6424
ER  - 
@conference{
author = "Lujić, Tamara and Gligorijević, Nikola and Jovanović, Vesna and Aćimović, Jelena and Mitić, Dragana and Vasović, Tamara and Stojadinović, Marija and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "Microplastics is abundant in the environment, food and beverages and get ingested by humans. Its complex interplay with proteins lead to formation of corona. Tightly bound proteins represent hard corona, while weaker binding partners are found in soft corona. Separation of hard and soft corona of allergenic proteins of shrimps, eggs and cow’s milk, tropomyosin (TPM), ovalbumin (OVA) and beta-lactoglobulin (BLG) and identification of binding partners by proteomics was aim of our study.
Allergenic proteins were purified from egg white, shrimps and cow’s milk. Binding to polyethylene terephthalate microplastics (PET) (70-100 m) was probed at pH 7 for purified allergens and egg white proteins. After establishment of binding equilibrium, soft and hard corona were separated and analyzed by SDS PAGE, followed by identification of bound proteins by nanoLC-HRMS. Binding of all allergenic proteins was observed in both soft and hard corona. Soft corona contains exclusively intact, full length OVA, TPM and BLG. Hard corona is enriched for truncated OVA and oligomers of TPM. OVA fragments are partially or fully enfolded and have higher level of exposed hydrophobic patches resulting in higher affinity for PET microplastics. In comparison to OVA and TPM, hard corona of BLG is less abundant under similar conditions. BLG is compact globular protein with lower level of exposed hydrophobic patches in comparison to ovalbumin and tropomyosin. In hard corona, trace amounts of contaminating alfa-lactalbumin become enriched. In the presence of egg white protein extract OVA forms both SC and HC on microplastics, being the dominant protein of hard corona (with ovotransferrin). Lysozyme and ovomucin are present only in hard corona. Both proteins are known for their strong bioactivity and represent a small fraction of total egg white proteins.
Our results show that allergenic proteins form hard corona on PET microplastics. Among egg white proteins, minor proteins such as lysozyme and ovomucin become enriched. Denaturing effect of strong binding to microplastics may change functional characteristics of allergens and bioactive proteins of foods and should be further investigated in functional assays.
Acknowledgment: This study was supported by IMPTOX European Union's Horizon 2020 research and innovation program (grant number 965173).",
publisher = "Italian Proteomics Association",
journal = "ItPA HPS and SePA XVII International Congress: Proteomics and Metabolomics towards Global Health, November 29th – December 1st, 2023, Roma, Italy",
title = "Proteomic insight into allergenic food corona on polyethylene terephthalate microplastics",
pages = "11-11",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6424"
}
Lujić, T., Gligorijević, N., Jovanović, V., Aćimović, J., Mitić, D., Vasović, T., Stojadinović, M., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2023). Proteomic insight into allergenic food corona on polyethylene terephthalate microplastics. in ItPA HPS and SePA XVII International Congress: Proteomics and Metabolomics towards Global Health, November 29th – December 1st, 2023, Roma, Italy
Italian Proteomics Association., 11-11.
https://hdl.handle.net/21.15107/rcub_cherry_6424
Lujić T, Gligorijević N, Jovanović V, Aćimović J, Mitić D, Vasović T, Stojadinović M, Stanić-Vučinić D, Ćirković-Veličković T. Proteomic insight into allergenic food corona on polyethylene terephthalate microplastics. in ItPA HPS and SePA XVII International Congress: Proteomics and Metabolomics towards Global Health, November 29th – December 1st, 2023, Roma, Italy. 2023;:11-11.
https://hdl.handle.net/21.15107/rcub_cherry_6424 .
Lujić, Tamara, Gligorijević, Nikola, Jovanović, Vesna, Aćimović, Jelena, Mitić, Dragana, Vasović, Tamara, Stojadinović, Marija, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Proteomic insight into allergenic food corona on polyethylene terephthalate microplastics" in ItPA HPS and SePA XVII International Congress: Proteomics and Metabolomics towards Global Health, November 29th – December 1st, 2023, Roma, Italy (2023):11-11,
https://hdl.handle.net/21.15107/rcub_cherry_6424 .

Redox status of critical disulfides of SARS-CoV-2 receptor-binding-domain exposed to bioactive chomophore phycocyanobilin

Simović, Ana; Bićanin, Maša; Radomirović, Mirjana Ž.; Aćimovič, Jelena; Mitić, Dragana; Stanić-Vučinić, Dragana; Ćirković-Veličković, Tanja

(Italian Proteomics Association, 2023)

TY  - CONF
AU  - Simović, Ana
AU  - Bićanin, Maša
AU  - Radomirović, Mirjana Ž.
AU  - Aćimovič, Jelena
AU  - Mitić, Dragana
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6418
AB  - The emergence of the novel coronavirus SARS-CoV-2 has attracted the attention of the whole scientific community. However, as there are significant concerns regarding the effectiveness of vaccines and drugs against novel SARS-CoV-2 variants, naturally derived broad-spectrum of antivirals seems to be precious adjuvant agents to assist in combat against this disease. Phycocyanobilin (PCB) is an open-chain tetrapyrrole chromophore of phycocyanin (PC), chromoprotein derived from Spirullina, with strong anti-oxidative action. The role of disulfide bonds and thiol-disulfide balance in RBD is considered to play a significant role in the binding of S protein to ACE2 receptor. In RBD, in contrast to C480–C488 disulfide, which is thermodynamically stable, C379–C432 and C391–C525 disulfides are in dynamic equilibrium with their thiol states and, thus these two pairs of disulfides are more sensitive to changes in redox poise. Our study aimed to investigate impact of PCB on disulfide balance of RBD by redox proteomics and to investigate structural changes in the protein exposed to PCB. The effect of PCB on RBD secondary structures was examined by far-UV CD spectroscopy after titration of RBD with increasing concentrations of PCB. The presence of PCB had a pronounced effect on the spectral shape. RBD is dominantly composed of random coils and β-sheets. In the presence of PCB a slight increase of α-helical and random coils content, while the content of β-sheets and β-turns is decreased. Mapping redox-active disulfides and reactive cysteines in recombinant SARS-CoV-2 RBD was done using redox proteomics on both recombinant RBD and PCB-exposed RBD. A mass shift caused by alkylation of free Cys residues was detected on three Cys residues demonstrating disulfides C379–C432 and 432-391 to be semi-stable in both RBD and PCB-exposed RBD. Our results demonstrate that RBD exposed to PCB undergo structural changes but does not change the redox state of its critical semi-stable disulfides.
Acknowledgment: The authors acknowledge support of the special Research Fund (BOF) of Ghent University (grant number 01N01718) and the Ministry of Science, Innovation and technological development of the Republic of Serbia (Contract number: 451-03-68/2023-14/200168).
PB  - Italian Proteomics Association
C3  - ItPA HPS and SePA XVII International Congress: Proteomics and Metabolomics towards Global Health, November 29th – December 1st, 2023, Roma, Italy
T1  - Redox status of critical disulfides of SARS-CoV-2 receptor-binding-domain exposed to bioactive chomophore phycocyanobilin
SP  - 63
EP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6418
ER  - 
@conference{
author = "Simović, Ana and Bićanin, Maša and Radomirović, Mirjana Ž. and Aćimovič, Jelena and Mitić, Dragana and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "The emergence of the novel coronavirus SARS-CoV-2 has attracted the attention of the whole scientific community. However, as there are significant concerns regarding the effectiveness of vaccines and drugs against novel SARS-CoV-2 variants, naturally derived broad-spectrum of antivirals seems to be precious adjuvant agents to assist in combat against this disease. Phycocyanobilin (PCB) is an open-chain tetrapyrrole chromophore of phycocyanin (PC), chromoprotein derived from Spirullina, with strong anti-oxidative action. The role of disulfide bonds and thiol-disulfide balance in RBD is considered to play a significant role in the binding of S protein to ACE2 receptor. In RBD, in contrast to C480–C488 disulfide, which is thermodynamically stable, C379–C432 and C391–C525 disulfides are in dynamic equilibrium with their thiol states and, thus these two pairs of disulfides are more sensitive to changes in redox poise. Our study aimed to investigate impact of PCB on disulfide balance of RBD by redox proteomics and to investigate structural changes in the protein exposed to PCB. The effect of PCB on RBD secondary structures was examined by far-UV CD spectroscopy after titration of RBD with increasing concentrations of PCB. The presence of PCB had a pronounced effect on the spectral shape. RBD is dominantly composed of random coils and β-sheets. In the presence of PCB a slight increase of α-helical and random coils content, while the content of β-sheets and β-turns is decreased. Mapping redox-active disulfides and reactive cysteines in recombinant SARS-CoV-2 RBD was done using redox proteomics on both recombinant RBD and PCB-exposed RBD. A mass shift caused by alkylation of free Cys residues was detected on three Cys residues demonstrating disulfides C379–C432 and 432-391 to be semi-stable in both RBD and PCB-exposed RBD. Our results demonstrate that RBD exposed to PCB undergo structural changes but does not change the redox state of its critical semi-stable disulfides.
Acknowledgment: The authors acknowledge support of the special Research Fund (BOF) of Ghent University (grant number 01N01718) and the Ministry of Science, Innovation and technological development of the Republic of Serbia (Contract number: 451-03-68/2023-14/200168).",
publisher = "Italian Proteomics Association",
journal = "ItPA HPS and SePA XVII International Congress: Proteomics and Metabolomics towards Global Health, November 29th – December 1st, 2023, Roma, Italy",
title = "Redox status of critical disulfides of SARS-CoV-2 receptor-binding-domain exposed to bioactive chomophore phycocyanobilin",
pages = "63-63",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6418"
}
Simović, A., Bićanin, M., Radomirović, M. Ž., Aćimovič, J., Mitić, D., Stanić-Vučinić, D.,& Ćirković-Veličković, T.. (2023). Redox status of critical disulfides of SARS-CoV-2 receptor-binding-domain exposed to bioactive chomophore phycocyanobilin. in ItPA HPS and SePA XVII International Congress: Proteomics and Metabolomics towards Global Health, November 29th – December 1st, 2023, Roma, Italy
Italian Proteomics Association., 63-63.
https://hdl.handle.net/21.15107/rcub_cherry_6418
Simović A, Bićanin M, Radomirović MŽ, Aćimovič J, Mitić D, Stanić-Vučinić D, Ćirković-Veličković T. Redox status of critical disulfides of SARS-CoV-2 receptor-binding-domain exposed to bioactive chomophore phycocyanobilin. in ItPA HPS and SePA XVII International Congress: Proteomics and Metabolomics towards Global Health, November 29th – December 1st, 2023, Roma, Italy. 2023;:63-63.
https://hdl.handle.net/21.15107/rcub_cherry_6418 .
Simović, Ana, Bićanin, Maša, Radomirović, Mirjana Ž., Aćimovič, Jelena, Mitić, Dragana, Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, "Redox status of critical disulfides of SARS-CoV-2 receptor-binding-domain exposed to bioactive chomophore phycocyanobilin" in ItPA HPS and SePA XVII International Congress: Proteomics and Metabolomics towards Global Health, November 29th – December 1st, 2023, Roma, Italy (2023):63-63,
https://hdl.handle.net/21.15107/rcub_cherry_6418 .