Stanisavljević, Nemanja S.

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  • Stanisavljević, Nemanja S. (4)
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Author's Bibliography

Burkholderia cepacia YtnP and Y2-aiiA lactonases inhibit virulence of Pseudomonas aeruginosa via quorum quenching activity

Malešević, Milka; Stanisavljević, Nemanja S.; Novović, Katarina; Polović, Natalija; Vasiljević, Zorica; Kojić, Milan O.; Jovčić, Branko

(Elsevier, 2020)

TY  - JOUR
AU  - Malešević, Milka
AU  - Stanisavljević, Nemanja S.
AU  - Novović, Katarina
AU  - Polović, Natalija
AU  - Vasiljević, Zorica
AU  - Kojić, Milan O.
AU  - Jovčić, Branko
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4257
AB  - Burkholderia cepacia is well known as the causative agent of infections in humans where often shares niche with other pathogens, like Pseudomonas aeruginosa. Clinical isolate Burkholderia sp. BCC4135 was selected due to its strong quorum quenching (QQ) activity. Whole genome sequencing unveiled this isolate as B. cepacia with unique sequence type ST1485 and a myriad of genes belonging to resistome and virulome. Two QQ lactonases YtnP and Y2-aiiA originated from B. cepacia BCC4135 were cloned, expressed, and functionally characterized. They were active against a broad substrate spectrum of the N-acyl-homoserine lactones (AHLs). The YtnP lactonase was inactive, while Y2-aiiA was active against N-tetradecanoyl-dl-homoserine lactone (C14-HSL) which could imply the difference in their biological roles from the aspect of its quorum sensing (QS) autoregulation and interference with the QS systems of bacteria residing within the same niche. Both YtnP and Y2-aiiA were able to attenuate virulence potential of P. aeruginosa MMA83 clinical isolate declining its biofilm formation and virulence factors production. B. cepacia BCC4135 lactonases interfered with the las, rhl, and even pqs QS circuit of P. aeruginosa MMA83 transcription and the effect of combined enzymes was even more prominent. B. cepacia BCC4135 also employs the CepI/R QS system for governing its own virulence traits and possibly self-regulates the QQ/QS network through the different expression and activity of YtnP and/or Y2-aiiA. Our findings pointed out that BCC4135 lactonases could be exploited as an effective antivirulence drugs against P. aeruginosa and gave us a new insight into B. cepacia QQ/QS machinery.
PB  - Elsevier
T2  - Microbial Pathogenesis
T1  - Burkholderia cepacia YtnP and Y2-aiiA lactonases inhibit virulence of Pseudomonas aeruginosa via quorum quenching activity
VL  - 149
DO  - 10.1016/j.micpath.2020.104561
ER  - 
@article{
author = "Malešević, Milka and Stanisavljević, Nemanja S. and Novović, Katarina and Polović, Natalija and Vasiljević, Zorica and Kojić, Milan O. and Jovčić, Branko",
year = "2020",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/4257",
abstract = "Burkholderia cepacia is well known as the causative agent of infections in humans where often shares niche with other pathogens, like Pseudomonas aeruginosa. Clinical isolate Burkholderia sp. BCC4135 was selected due to its strong quorum quenching (QQ) activity. Whole genome sequencing unveiled this isolate as B. cepacia with unique sequence type ST1485 and a myriad of genes belonging to resistome and virulome. Two QQ lactonases YtnP and Y2-aiiA originated from B. cepacia BCC4135 were cloned, expressed, and functionally characterized. They were active against a broad substrate spectrum of the N-acyl-homoserine lactones (AHLs). The YtnP lactonase was inactive, while Y2-aiiA was active against N-tetradecanoyl-dl-homoserine lactone (C14-HSL) which could imply the difference in their biological roles from the aspect of its quorum sensing (QS) autoregulation and interference with the QS systems of bacteria residing within the same niche. Both YtnP and Y2-aiiA were able to attenuate virulence potential of P. aeruginosa MMA83 clinical isolate declining its biofilm formation and virulence factors production. B. cepacia BCC4135 lactonases interfered with the las, rhl, and even pqs QS circuit of P. aeruginosa MMA83 transcription and the effect of combined enzymes was even more prominent. B. cepacia BCC4135 also employs the CepI/R QS system for governing its own virulence traits and possibly self-regulates the QQ/QS network through the different expression and activity of YtnP and/or Y2-aiiA. Our findings pointed out that BCC4135 lactonases could be exploited as an effective antivirulence drugs against P. aeruginosa and gave us a new insight into B. cepacia QQ/QS machinery.",
publisher = "Elsevier",
journal = "Microbial Pathogenesis",
title = "Burkholderia cepacia YtnP and Y2-aiiA lactonases inhibit virulence of Pseudomonas aeruginosa via quorum quenching activity",
volume = "149",
doi = "10.1016/j.micpath.2020.104561"
}
Malešević, M., Stanisavljević, N. S., Novović, K., Polović, N., Vasiljević, Z., Kojić, M. O.,& Jovčić, B. (2020). Burkholderia cepacia YtnP and Y2-aiiA lactonases inhibit virulence of Pseudomonas aeruginosa via quorum quenching activity.
Microbial Pathogenesis
Elsevier., 149.
https://doi.org/10.1016/j.micpath.2020.104561
Malešević M, Stanisavljević NS, Novović K, Polović N, Vasiljević Z, Kojić MO, Jovčić B. Burkholderia cepacia YtnP and Y2-aiiA lactonases inhibit virulence of Pseudomonas aeruginosa via quorum quenching activity. Microbial Pathogenesis. 2020;149
Malešević Milka, Stanisavljević Nemanja S., Novović Katarina, Polović Natalija, Vasiljević Zorica, Kojić Milan O., Jovčić Branko, "Burkholderia cepacia YtnP and Y2-aiiA lactonases inhibit virulence of Pseudomonas aeruginosa via quorum quenching activity" Microbial Pathogenesis, 149 (2020),
https://doi.org/10.1016/j.micpath.2020.104561 .
1
2
1

Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix

Stanisavljević, Nemanja S.; Soković Bajić, Svetlana; Jovanović, Živko; Matić, Ivana; Tolinački, Maja; Popović, Dušanka; Popović, Nikola; Terzić-Vidojević, Amarela; Golić, Nataša; Beškoski, Vladimir; Samardžić, Jelena

(Frontiers, 2020)

TY  - JOUR
AU  - Stanisavljević, Nemanja S.
AU  - Soković Bajić, Svetlana
AU  - Jovanović, Živko
AU  - Matić, Ivana
AU  - Tolinački, Maja
AU  - Popović, Dušanka
AU  - Popović, Nikola
AU  - Terzić-Vidojević, Amarela
AU  - Golić, Nataša
AU  - Beškoski, Vladimir
AU  - Samardžić, Jelena
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4300
AB  - In this study, for the first time, the comprehensive analysis of antiproliferative and antioxidant activities of ramson, followed by the analysis of its associated microbiota and health-promoting effects of lactic acid bacteria (LAB), was performed. Ramson (Allium ursinum) is recognized as a medicinal plant with a long history of use in traditional medicine due to its antimicrobial and antioxidant activity. In this study the influence of in vitro gastrointestinal digestion on the cytotoxic activity of A. ursinum extracts against human malignant cell lines was demonstrated. Seven sulfur compounds, the degradation products of thiosulfinates, including diallyl disulfide were shown to inhibit proliferation of malignant cells by inducing accumulation within G2/M phase as well as to induce apoptosis through activation of caspase-3 and mitochondrial signaling pathway. Further, the A. ursinum microbiota, particularly LAB with potential probiotic effects, was analyzed by culture-dependent method and culture-independent method [denaturing gradient gel electrophoresis (DGGE)]. The obtained results revealed that the most abundant genera were Streptococcus, Lactobacillus, and Bacillus. The Lactobacillus genus was mainly represented by L. fermentum. The pulsed-field gel electrophoresis (PFGE) analysis revealed the presence of two PFGE pulsotypes. The probiotic potential of the strain L. fermentum BGSR163 belonging to PFGE pulsotype 1 and the strain L. fermentum BGSR227 belonging to the PFGE pulsotype 2 was characterized. The results revealed that both strains are safe for human use, successfully survive the simulated gastrointestinal conditions, have potential to transiently colonize the gastrointestinal tract (GIT) and have a protective immunomodulatory effect, inducing the production of proinflammatory cytokine IL17 and regulatory cytokine IL10, while decreasing the production of proinflammatory cytokine IFN-γ. In conclusion, the results of this study suggest that consumption of A. ursinum might have health-promoting properties, including anticancer effects, while L. fermentum strains isolated from A. ursinum leaves could be used as probiotics for human consumption.
PB  - Frontiers
T2  - Frontiers in Microbiology
T1  - Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix
VL  - 11
DO  - 10.3389/fmicb.2020.601616
ER  - 
@article{
author = "Stanisavljević, Nemanja S. and Soković Bajić, Svetlana and Jovanović, Živko and Matić, Ivana and Tolinački, Maja and Popović, Dušanka and Popović, Nikola and Terzić-Vidojević, Amarela and Golić, Nataša and Beškoski, Vladimir and Samardžić, Jelena",
year = "2020",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/4300",
abstract = "In this study, for the first time, the comprehensive analysis of antiproliferative and antioxidant activities of ramson, followed by the analysis of its associated microbiota and health-promoting effects of lactic acid bacteria (LAB), was performed. Ramson (Allium ursinum) is recognized as a medicinal plant with a long history of use in traditional medicine due to its antimicrobial and antioxidant activity. In this study the influence of in vitro gastrointestinal digestion on the cytotoxic activity of A. ursinum extracts against human malignant cell lines was demonstrated. Seven sulfur compounds, the degradation products of thiosulfinates, including diallyl disulfide were shown to inhibit proliferation of malignant cells by inducing accumulation within G2/M phase as well as to induce apoptosis through activation of caspase-3 and mitochondrial signaling pathway. Further, the A. ursinum microbiota, particularly LAB with potential probiotic effects, was analyzed by culture-dependent method and culture-independent method [denaturing gradient gel electrophoresis (DGGE)]. The obtained results revealed that the most abundant genera were Streptococcus, Lactobacillus, and Bacillus. The Lactobacillus genus was mainly represented by L. fermentum. The pulsed-field gel electrophoresis (PFGE) analysis revealed the presence of two PFGE pulsotypes. The probiotic potential of the strain L. fermentum BGSR163 belonging to PFGE pulsotype 1 and the strain L. fermentum BGSR227 belonging to the PFGE pulsotype 2 was characterized. The results revealed that both strains are safe for human use, successfully survive the simulated gastrointestinal conditions, have potential to transiently colonize the gastrointestinal tract (GIT) and have a protective immunomodulatory effect, inducing the production of proinflammatory cytokine IL17 and regulatory cytokine IL10, while decreasing the production of proinflammatory cytokine IFN-γ. In conclusion, the results of this study suggest that consumption of A. ursinum might have health-promoting properties, including anticancer effects, while L. fermentum strains isolated from A. ursinum leaves could be used as probiotics for human consumption.",
publisher = "Frontiers",
journal = "Frontiers in Microbiology",
title = "Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix",
volume = "11",
doi = "10.3389/fmicb.2020.601616"
}
Stanisavljević, N. S., Soković Bajić, S., Jovanović, Ž., Matić, I., Tolinački, M., Popović, D., Popović, N., Terzić-Vidojević, A., Golić, N., Beškoski, V.,& Samardžić, J. (2020). Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix.
Frontiers in Microbiology
Frontiers., 11.
https://doi.org/10.3389/fmicb.2020.601616
Stanisavljević NS, Soković Bajić S, Jovanović Ž, Matić I, Tolinački M, Popović D, Popović N, Terzić-Vidojević A, Golić N, Beškoski V, Samardžić J. Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix. Frontiers in Microbiology. 2020;11
Stanisavljević Nemanja S., Soković Bajić Svetlana, Jovanović Živko, Matić Ivana, Tolinački Maja, Popović Dušanka, Popović Nikola, Terzić-Vidojević Amarela, Golić Nataša, Beškoski Vladimir, Samardžić Jelena, "Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix" Frontiers in Microbiology, 11 (2020),
https://doi.org/10.3389/fmicb.2020.601616 .
1
2
2

Nutraceutical properties and phytochemical characterization of wild Serbian fruits

Natić, Maja; Pavlović, Aleksandra; Bosco, Fabrizia Lo; Stanisavljević, Nemanja S.; Dabić-Zagorac, Dragana; Fotirić-Akšić, Milica M.; Papetti, Adele

(Springer, 2019)

TY  - JOUR
AU  - Natić, Maja
AU  - Pavlović, Aleksandra
AU  - Bosco, Fabrizia Lo
AU  - Stanisavljević, Nemanja S.
AU  - Dabić-Zagorac, Dragana
AU  - Fotirić-Akšić, Milica M.
AU  - Papetti, Adele
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3755
AB  - Wild fruits grown in Serbia, i.e., elderberry (Sambucus nigra), hawthorn (Crataegus monogyna), cornelian cherry (Cornus mas), and blackthorn (Prunus spinosa), are rich in secondary metabolites. In this study, the polyphenolic composition of wild fruit extracts and their antioxidant capacity were investigated by in vitro assays. Elderberry was characterized by the presence of arbutin (a skin protector), and cornelian cherry by syringic and gallic acids. In blackthorn, at least 11 different phenolic compounds were reported for the first time, including vanillic acid and naringin, the metabolite present in the highest amount. Blackthorn extracts were the richest in polyphenols (11.24–18.70 g GAE/kg FW) and had the highest activity in the DPPH radical test (180.93–267.11 mMTE/mL), while cornelian cherry extracts showed the most effective ferric ion chelating (81.37–90.66%) and antityrosinase inhibition capacities (21.75–74.23%). No sample was able to scavenge NO. Using the principal component analysis, wild fruit samples were classified into four separate clusters due to distinctive phenolic profiles and antioxidant capacity. Our investigation showed how every fruit could be considered unique in terms of its phytonutrient content. Thus, Serbian wild fruits may be a great source of bioactive natural compounds and could be therefore considered particularly useful in food supplement production. Particularly, as a source of natural antioxidants, these species could be used to extend the shelf life of food products and replace synthetic antioxidants, avoiding potential health risks and toxicity.
PB  - Springer
T2  - European Food Research and Technology
T1  - Nutraceutical properties and phytochemical characterization of wild Serbian fruits
VL  - 245
IS  - 2
SP  - 469
EP  - 478
DO  - 10.1007/s00217-018-3178-1
ER  - 
@article{
author = "Natić, Maja and Pavlović, Aleksandra and Bosco, Fabrizia Lo and Stanisavljević, Nemanja S. and Dabić-Zagorac, Dragana and Fotirić-Akšić, Milica M. and Papetti, Adele",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3755",
abstract = "Wild fruits grown in Serbia, i.e., elderberry (Sambucus nigra), hawthorn (Crataegus monogyna), cornelian cherry (Cornus mas), and blackthorn (Prunus spinosa), are rich in secondary metabolites. In this study, the polyphenolic composition of wild fruit extracts and their antioxidant capacity were investigated by in vitro assays. Elderberry was characterized by the presence of arbutin (a skin protector), and cornelian cherry by syringic and gallic acids. In blackthorn, at least 11 different phenolic compounds were reported for the first time, including vanillic acid and naringin, the metabolite present in the highest amount. Blackthorn extracts were the richest in polyphenols (11.24–18.70 g GAE/kg FW) and had the highest activity in the DPPH radical test (180.93–267.11 mMTE/mL), while cornelian cherry extracts showed the most effective ferric ion chelating (81.37–90.66%) and antityrosinase inhibition capacities (21.75–74.23%). No sample was able to scavenge NO. Using the principal component analysis, wild fruit samples were classified into four separate clusters due to distinctive phenolic profiles and antioxidant capacity. Our investigation showed how every fruit could be considered unique in terms of its phytonutrient content. Thus, Serbian wild fruits may be a great source of bioactive natural compounds and could be therefore considered particularly useful in food supplement production. Particularly, as a source of natural antioxidants, these species could be used to extend the shelf life of food products and replace synthetic antioxidants, avoiding potential health risks and toxicity.",
publisher = "Springer",
journal = "European Food Research and Technology",
title = "Nutraceutical properties and phytochemical characterization of wild Serbian fruits",
volume = "245",
number = "2",
pages = "469-478",
doi = "10.1007/s00217-018-3178-1"
}
Natić, M., Pavlović, A., Bosco, F. L., Stanisavljević, N. S., Dabić-Zagorac, D., Fotirić-Akšić, M. M.,& Papetti, A. (2019). Nutraceutical properties and phytochemical characterization of wild Serbian fruits.
European Food Research and Technology
Springer., 245(2), 469-478.
https://doi.org/10.1007/s00217-018-3178-1
Natić M, Pavlović A, Bosco FL, Stanisavljević NS, Dabić-Zagorac D, Fotirić-Akšić MM, Papetti A. Nutraceutical properties and phytochemical characterization of wild Serbian fruits. European Food Research and Technology. 2019;245(2):469-478
Natić Maja, Pavlović Aleksandra, Bosco Fabrizia Lo, Stanisavljević Nemanja S., Dabić-Zagorac Dragana, Fotirić-Akšić Milica M., Papetti Adele, "Nutraceutical properties and phytochemical characterization of wild Serbian fruits" European Food Research and Technology, 245, no. 2 (2019):469-478,
https://doi.org/10.1007/s00217-018-3178-1 .
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9

Supplementary data for article: Natić, M.; Pavlović, A.; Bosco, F. L.; Stanisavljević, N.; Zagorac, D. D.; Akšić, M. F.; Papetti, A. Nutraceutical Properties and Phytochemical Characterization of Wild Serbian Fruits. European Food Research and Technology 2019, 245 (2), 469–478. https://doi.org/10.1007/s00217-018-3178-1

Natić, Maja; Pavlović, Aleksandra; Bosco, Fabrizia Lo; Stanisavljević, Nemanja S.; Dabić-Zagorac, Dragana; Fotirić-Akšić, Milica M.; Papetti, Adele

(Springer, 2019)

TY  - BOOK
AU  - Natić, Maja
AU  - Pavlović, Aleksandra
AU  - Bosco, Fabrizia Lo
AU  - Stanisavljević, Nemanja S.
AU  - Dabić-Zagorac, Dragana
AU  - Fotirić-Akšić, Milica M.
AU  - Papetti, Adele
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3756
PB  - Springer
T2  - European Food Research and Technology
T1  - Supplementary data for article: Natić, M.; Pavlović, A.; Bosco, F. L.; Stanisavljević, N.; Zagorac, D. D.; Akšić, M. F.; Papetti, A. Nutraceutical Properties and Phytochemical Characterization of Wild Serbian Fruits. European Food Research and Technology 2019, 245 (2), 469–478. https://doi.org/10.1007/s00217-018-3178-1
ER  - 
@book{
author = "Natić, Maja and Pavlović, Aleksandra and Bosco, Fabrizia Lo and Stanisavljević, Nemanja S. and Dabić-Zagorac, Dragana and Fotirić-Akšić, Milica M. and Papetti, Adele",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3756",
publisher = "Springer",
journal = "European Food Research and Technology",
title = "Supplementary data for article: Natić, M.; Pavlović, A.; Bosco, F. L.; Stanisavljević, N.; Zagorac, D. D.; Akšić, M. F.; Papetti, A. Nutraceutical Properties and Phytochemical Characterization of Wild Serbian Fruits. European Food Research and Technology 2019, 245 (2), 469–478. https://doi.org/10.1007/s00217-018-3178-1"
}
Natić, M., Pavlović, A., Bosco, F. L., Stanisavljević, N. S., Dabić-Zagorac, D., Fotirić-Akšić, M. M.,& Papetti, A. (2019). Supplementary data for article: Natić, M.; Pavlović, A.; Bosco, F. L.; Stanisavljević, N.; Zagorac, D. D.; Akšić, M. F.; Papetti, A. Nutraceutical Properties and Phytochemical Characterization of Wild Serbian Fruits. European Food Research and Technology 2019, 245 (2), 469–478. https://doi.org/10.1007/s00217-018-3178-1.
European Food Research and Technology
Springer..
Natić M, Pavlović A, Bosco FL, Stanisavljević NS, Dabić-Zagorac D, Fotirić-Akšić MM, Papetti A. Supplementary data for article: Natić, M.; Pavlović, A.; Bosco, F. L.; Stanisavljević, N.; Zagorac, D. D.; Akšić, M. F.; Papetti, A. Nutraceutical Properties and Phytochemical Characterization of Wild Serbian Fruits. European Food Research and Technology 2019, 245 (2), 469–478. https://doi.org/10.1007/s00217-018-3178-1. European Food Research and Technology. 2019;
Natić Maja, Pavlović Aleksandra, Bosco Fabrizia Lo, Stanisavljević Nemanja S., Dabić-Zagorac Dragana, Fotirić-Akšić Milica M., Papetti Adele, "Supplementary data for article: Natić, M.; Pavlović, A.; Bosco, F. L.; Stanisavljević, N.; Zagorac, D. D.; Akšić, M. F.; Papetti, A. Nutraceutical Properties and Phytochemical Characterization of Wild Serbian Fruits. European Food Research and Technology 2019, 245 (2), 469–478. https://doi.org/10.1007/s00217-018-3178-1" European Food Research and Technology (2019)