TY  - JOUR
AU  - Vukojević, Vesna
AU  - Đurđić, Slađana Z.
AU  - Mutić, Jelena
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3283
AB  - Cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium can be used in lactobionic acid production, biosensor for lactose, biofuel cells, lignocellulose degradation, and wound-healing applications. To make it a better biocatalyst, CDH with higher activity in an immobilized form is desirable. For this purpose, CDH was expressed for the first time on the surface of S. cerevisiae EBY100 cells in an active form as a triple mutant tmCDH (D20N, A64T, V592M) and evolved further for higher activity using resazurin-based fluorescent assay. In order to decrease blank reaction of resazurin with yeast cells and to have linear correlation between enzyme activity on the cell surface and fluorescence signal, the assay was optimized with respect to resazurin concentration (0.1 mM), substrate concentration (10mMlactose and 0.08mMcellobiose), and pH (6.0). Using optimized assay an error prone PCR gene library of tmCDH was screened. Two mutants with 5 (H5) and 7 mutations (H9) were found having two times higher activity than the parent tmCDH enzyme that already had improved activity compared to wild type CDH whose activity could not be detected on the surface of yeast cells.
T2  - Environmental Science and Pollution Research
T1  - Accumulation of U, Th, Pb, V, Rb, and Ag in wild Mushrooms macrolepiota procera (Scop.) Singer from Goc, Serbia
VL  - 26
IS  - 13
SP  - 13147
EP  - 13158
DO  - 10.1007/s11356-019-04723-z
ER  - 

@article{
author = "Vukojević, Vesna and Đurđić, Slađana Z. and Mutić, Jelena",
year = "2019",
abstract = "Cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium can be used in lactobionic acid production, biosensor for lactose, biofuel cells, lignocellulose degradation, and wound-healing applications. To make it a better biocatalyst, CDH with higher activity in an immobilized form is desirable. For this purpose, CDH was expressed for the first time on the surface of S. cerevisiae EBY100 cells in an active form as a triple mutant tmCDH (D20N, A64T, V592M) and evolved further for higher activity using resazurin-based fluorescent assay. In order to decrease blank reaction of resazurin with yeast cells and to have linear correlation between enzyme activity on the cell surface and fluorescence signal, the assay was optimized with respect to resazurin concentration (0.1 mM), substrate concentration (10mMlactose and 0.08mMcellobiose), and pH (6.0). Using optimized assay an error prone PCR gene library of tmCDH was screened. Two mutants with 5 (H5) and 7 mutations (H9) were found having two times higher activity than the parent tmCDH enzyme that already had improved activity compared to wild type CDH whose activity could not be detected on the surface of yeast cells.",
journal = "Environmental Science and Pollution Research",
title = "Accumulation of U, Th, Pb, V, Rb, and Ag in wild Mushrooms macrolepiota procera (Scop.) Singer from Goc, Serbia",
volume = "26",
number = "13",
pages = "13147-13158",
doi = "10.1007/s11356-019-04723-z"
}

Vukojević, V., Đurđić, S. Z.,& Mutić, J.. (2019). Accumulation of U, Th, Pb, V, Rb, and Ag in wild Mushrooms macrolepiota procera (Scop.) Singer from Goc, Serbia. in Environmental Science and Pollution Research, 26(13), 13147-13158.
https://doi.org/10.1007/s11356-019-04723-z

Vukojević V, Đurđić SZ, Mutić J. Accumulation of U, Th, Pb, V, Rb, and Ag in wild Mushrooms macrolepiota procera (Scop.) Singer from Goc, Serbia. in Environmental Science and Pollution Research. 2019;26(13):13147-13158.
doi:10.1007/s11356-019-04723-z .

Vukojević, Vesna, Đurđić, Slađana Z., Mutić, Jelena, "Accumulation of U, Th, Pb, V, Rb, and Ag in wild Mushrooms macrolepiota procera (Scop.) Singer from Goc, Serbia" in Environmental Science and Pollution Research, 26, no. 13 (2019):13147-13158,
https://doi.org/10.1007/s11356-019-04723-z . .

TY  - JOUR
AU  - Dimitrijević, Marija V.
AU  - Mitić, Violeta D.
AU  - Nikolić, Jelena S.
AU  - Djordjevic, Aleksandra S.
AU  - Mutić, Jelena
AU  - Stankov-Jovanović, Vesna P.
AU  - Stojanovic, Gordana S.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2844
AB  - The goal of this research was a comprehensive analysis of four wild edible mushroom species, Cantharellus cinereus, Clavariadelphus pistillaris, Clitocybe nebularis and Hygrocybe punicea, which have not been analyzed so far. Extracts of different polarities have been prepared and evaluated for their antioxidant activities by DPPH, ABTS, FRAP, TRP and CUPRAC methods. For all extracts, total phenolic content was determined. Based on the analysis, it was shown that solvent type had a significant effect on the antioxidant capacities of mushroom extracts, so water extracts showed the highest activity. Furthermore, the analysis includes determination of mineral composition, fatty acid profiles and antimicrobial activity. Unsaturated fatty acids, which are very important for human health, are dominant in the studied mushroom species. Linoleic and oleic acid consist of over 50 % of the total fatty acid composition. Seventeen biologically important and toxic elements have been analyzed by ICP-OES and ICP-MS and results showed that the element concentrations were species-dependent. Also, it has been found that analyzed mushrooms did not show any antimicrobial activity. Chemometric analysis was used to understand the connection between the extracts of different polarities.
PB  - Wiley
T2  - Chemistry and Biodiversity
T1  - First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms
VL  - 16
IS  - 2
SP  - 2
EP  - 11
DO  - 10.1002/cbdv.201800492
ER  - 

@article{
author = "Dimitrijević, Marija V. and Mitić, Violeta D. and Nikolić, Jelena S. and Djordjevic, Aleksandra S. and Mutić, Jelena and Stankov-Jovanović, Vesna P. and Stojanovic, Gordana S.",
year = "2019",
abstract = "The goal of this research was a comprehensive analysis of four wild edible mushroom species, Cantharellus cinereus, Clavariadelphus pistillaris, Clitocybe nebularis and Hygrocybe punicea, which have not been analyzed so far. Extracts of different polarities have been prepared and evaluated for their antioxidant activities by DPPH, ABTS, FRAP, TRP and CUPRAC methods. For all extracts, total phenolic content was determined. Based on the analysis, it was shown that solvent type had a significant effect on the antioxidant capacities of mushroom extracts, so water extracts showed the highest activity. Furthermore, the analysis includes determination of mineral composition, fatty acid profiles and antimicrobial activity. Unsaturated fatty acids, which are very important for human health, are dominant in the studied mushroom species. Linoleic and oleic acid consist of over 50 % of the total fatty acid composition. Seventeen biologically important and toxic elements have been analyzed by ICP-OES and ICP-MS and results showed that the element concentrations were species-dependent. Also, it has been found that analyzed mushrooms did not show any antimicrobial activity. Chemometric analysis was used to understand the connection between the extracts of different polarities.",
publisher = "Wiley",
journal = "Chemistry and Biodiversity",
title = "First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms",
volume = "16",
number = "2",
pages = "2-11",
doi = "10.1002/cbdv.201800492"
}

Dimitrijević, M. V., Mitić, V. D., Nikolić, J. S., Djordjevic, A. S., Mutić, J., Stankov-Jovanović, V. P.,& Stojanovic, G. S.. (2019). First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms. in Chemistry and Biodiversity
Wiley., 16(2), 2-11.
https://doi.org/10.1002/cbdv.201800492

Dimitrijević MV, Mitić VD, Nikolić JS, Djordjevic AS, Mutić J, Stankov-Jovanović VP, Stojanovic GS. First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms. in Chemistry and Biodiversity. 2019;16(2):2-11.
doi:10.1002/cbdv.201800492 .

Dimitrijević, Marija V., Mitić, Violeta D., Nikolić, Jelena S., Djordjevic, Aleksandra S., Mutić, Jelena, Stankov-Jovanović, Vesna P., Stojanovic, Gordana S., "First Report about Mineral Content, Fatty Acids Composition and Biological Activities of Four Wild Edible Mushrooms" in Chemistry and Biodiversity, 16, no. 2 (2019):2-11,
https://doi.org/10.1002/cbdv.201800492 . .

TY  - DATA
AU  - Đorđević, Neda O.
AU  - Novaković, M.
AU  - Pejin, Boris
AU  - Zivkovic, M.
AU  - Savić, Aleksandar
AU  - Mutić, Jelena
AU  - Tešević, Vele
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3015
PB  - Elsevier Science Bv, Amsterdam
T2  - Scientia Horticulturae
T1  - Supplementary data for the article: Đorđević, N.; Novaković, M.; Pejin, B.; Živković, M.; Savić, A.; Mutić, J.; Tešević, V. An Insight into Chemical Composition and Biological Activity of Montenegrin Vranac Red Wine. Scientia Horticulturae 2018, 230, 142–148. https://doi.org/10.1016/j.scienta.2017.11.033
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3015
ER  - 

@misc{
author = "Đorđević, Neda O. and Novaković, M. and Pejin, Boris and Zivkovic, M. and Savić, Aleksandar and Mutić, Jelena and Tešević, Vele",
year = "2018",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Scientia Horticulturae",
title = "Supplementary data for the article: Đorđević, N.; Novaković, M.; Pejin, B.; Živković, M.; Savić, A.; Mutić, J.; Tešević, V. An Insight into Chemical Composition and Biological Activity of Montenegrin Vranac Red Wine. Scientia Horticulturae 2018, 230, 142–148. https://doi.org/10.1016/j.scienta.2017.11.033",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3015"
}

Đorđević, N. O., Novaković, M., Pejin, B., Zivkovic, M., Savić, A., Mutić, J.,& Tešević, V.. (2018). Supplementary data for the article: Đorđević, N.; Novaković, M.; Pejin, B.; Živković, M.; Savić, A.; Mutić, J.; Tešević, V. An Insight into Chemical Composition and Biological Activity of Montenegrin Vranac Red Wine. Scientia Horticulturae 2018, 230, 142–148. https://doi.org/10.1016/j.scienta.2017.11.033. in Scientia Horticulturae
Elsevier Science Bv, Amsterdam..
https://hdl.handle.net/21.15107/rcub_cherry_3015

Đorđević NO, Novaković M, Pejin B, Zivkovic M, Savić A, Mutić J, Tešević V. Supplementary data for the article: Đorđević, N.; Novaković, M.; Pejin, B.; Živković, M.; Savić, A.; Mutić, J.; Tešević, V. An Insight into Chemical Composition and Biological Activity of Montenegrin Vranac Red Wine. Scientia Horticulturae 2018, 230, 142–148. https://doi.org/10.1016/j.scienta.2017.11.033. in Scientia Horticulturae. 2018;.
https://hdl.handle.net/21.15107/rcub_cherry_3015 .

Đorđević, Neda O., Novaković, M., Pejin, Boris, Zivkovic, M., Savić, Aleksandar, Mutić, Jelena, Tešević, Vele, "Supplementary data for the article: Đorđević, N.; Novaković, M.; Pejin, B.; Živković, M.; Savić, A.; Mutić, J.; Tešević, V. An Insight into Chemical Composition and Biological Activity of Montenegrin Vranac Red Wine. Scientia Horticulturae 2018, 230, 142–148. https://doi.org/10.1016/j.scienta.2017.11.033" in Scientia Horticulturae (2018),
https://hdl.handle.net/21.15107/rcub_cherry_3015 .