Maksimović, Vuk

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orcid::0000-0002-9883-186X
  • Maksimović, Vuk (4)
  • Maksimovic, Vuk (1)
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Author's Bibliography

Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose–response relationship

Bartolić, Dragana; Maksimović, Vuk; Maksimović, Jelena D.; Stanković, Mira; Krstović, Saša; Baošić, Rada; Radotić, Ksenija

(Wiley, 2020)

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Maksimović, Vuk
AU  - Maksimović, Jelena D.
AU  - Stanković, Mira
AU  - Krstović, Saša
AU  - Baošić, Rada
AU  - Radotić, Ksenija
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3949
AB  - BACKGROUND: Cereal seeds, such as maize seeds, are frequently contaminated with aflatoxin B1 (AFB1), one of the most dangerous naturally occurring carcinogens. In plants, phenolamides are involved in biotic stress response. The data on variations of phenolamides in AFB1-containing seeds are limited. RESULTS: Five polyamine conjugates, including two spermidine and three putrescine conjugates, were tentatively identified in methanolic extracts, using HPLC-DAD-MS. The ratio of putrescine to spermidine conjugates changed with increasing AFB1 concentration in a logistic dose–response manner, with a ratio of below 1 up to a concentration of 51.51 μg kg−1, and approximately 2.54 and 3 at higher concentrations of 177.4 and 308.13 μg kg−1, respectively. The observed variations of the total antioxidant activity and the total phenolic content may support this biphasic behaviour of the seeds against AFB1 stress. CONCLUSIONS: The obtained data are a contribution to the understanding of the roles of polyamine conjugates in seed defence to increasing AFB1 concentrations. According to our knowledge, this study reports for the first time the biphasic response of maize seeds to increasing AFB1 contamination level, comprising the induction of polyamine conjugate accumulation and variation in the ratio of conjugates. This dose–response relationship may provide useful information in the field of agricultural and food chemistry as an indicator of AFB1 contamination level and, hence, for selecting an appropriate seed quality.
PB  - Wiley
T2  - Journal of the Science of Food and Agriculture
T1  - Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose–response relationship
VL  - 100
IS  - 7
SP  - 2905
EP  - 2910
DO  - 10.1002/jsfa.10317
ER  - 
@article{
author = "Bartolić, Dragana and Maksimović, Vuk and Maksimović, Jelena D. and Stanković, Mira and Krstović, Saša and Baošić, Rada and Radotić, Ksenija",
year = "2020",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3949",
abstract = "BACKGROUND: Cereal seeds, such as maize seeds, are frequently contaminated with aflatoxin B1 (AFB1), one of the most dangerous naturally occurring carcinogens. In plants, phenolamides are involved in biotic stress response. The data on variations of phenolamides in AFB1-containing seeds are limited. RESULTS: Five polyamine conjugates, including two spermidine and three putrescine conjugates, were tentatively identified in methanolic extracts, using HPLC-DAD-MS. The ratio of putrescine to spermidine conjugates changed with increasing AFB1 concentration in a logistic dose–response manner, with a ratio of below 1 up to a concentration of 51.51 μg kg−1, and approximately 2.54 and 3 at higher concentrations of 177.4 and 308.13 μg kg−1, respectively. The observed variations of the total antioxidant activity and the total phenolic content may support this biphasic behaviour of the seeds against AFB1 stress. CONCLUSIONS: The obtained data are a contribution to the understanding of the roles of polyamine conjugates in seed defence to increasing AFB1 concentrations. According to our knowledge, this study reports for the first time the biphasic response of maize seeds to increasing AFB1 contamination level, comprising the induction of polyamine conjugate accumulation and variation in the ratio of conjugates. This dose–response relationship may provide useful information in the field of agricultural and food chemistry as an indicator of AFB1 contamination level and, hence, for selecting an appropriate seed quality.",
publisher = "Wiley",
journal = "Journal of the Science of Food and Agriculture",
title = "Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose–response relationship",
volume = "100",
number = "7",
pages = "2905-2910",
doi = "10.1002/jsfa.10317"
}
Bartolić, D., Maksimović, V., Maksimović, J. D., Stanković, M., Krstović, S., Baošić, R.,& Radotić, K. (2020). Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose–response relationship.
Journal of the Science of Food and Agriculture
Wiley., 100(7), 2905-2910.
https://doi.org/10.1002/jsfa.10317
Bartolić D, Maksimović V, Maksimović JD, Stanković M, Krstović S, Baošić R, Radotić K. Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose–response relationship. Journal of the Science of Food and Agriculture. 2020;100(7):2905-2910
Bartolić Dragana, Maksimović Vuk, Maksimović Jelena D., Stanković Mira, Krstović Saša, Baošić Rada, Radotić Ksenija, "Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose–response relationship" Journal of the Science of Food and Agriculture, 100, no. 7 (2020):2905-2910,
https://doi.org/10.1002/jsfa.10317 .
2
1

Interaction of carbohydrate coated cerium-oxide nanoparticles with wheat and pea: Stress induction potential and effect on development

Milenković, Ivana; Mitrović, Aleksandra D.; Algarra, Manuel; Lázaro-Martínez, Juan M.; Rodríguez-Castellón, Enrique; Maksimović, Vuk; Spasić, Slađana Z.; Beškoski, Vladimir; Radotić, Ksenija

(MDPI, 2019)

TY  - JOUR
AU  - Milenković, Ivana
AU  - Mitrović, Aleksandra D.
AU  - Algarra, Manuel
AU  - Lázaro-Martínez, Juan M.
AU  - Rodríguez-Castellón, Enrique
AU  - Maksimović, Vuk
AU  - Spasić, Slađana Z.
AU  - Beškoski, Vladimir
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3698
AB  - Reports about the influence of cerium-oxide nanoparticles (nCeO2) on plants are contradictory due to their positive and negative effects on plants. Surface modification may affect the interaction of nCeO2 with the environment, and hence its availability to plants. In this study, the uncoated and glucose-, levan-, and pullulan-coated nCeO2 were synthesized and characterized. The aim was to determine whether nontoxic carbohydrates alter the effect of nCeO2 on the seed germination, plant growth, and metabolism of wheat and pea. We applied 200 mgL-1 of nCeO2 on plants during germination (Ger treatment) or three week-growth (Gro treatment) in hydroponics. The plant response to nCeO2 was studied by measuring changes in Ce concentration, total antioxidative activity (TAA), total phenolic content (TPC), and phenolic profile. Our results generally revealed higher Ce concentration in plants after the treatment with coated nanoparticles compared to uncoated ones. Considering all obtained results, Ger treatment had a stronger impact on the later stages of plant development than Gro treatment. The Ger treatment had a stronger impact on TPC and plant elongation, whereas Gro treatment affected more TAA and phenolic profile. Among nanoparticles, levan-coated nCeO2 had the strongest and positive impact on tested plants. Wheat showed higher sensitivity to all treatments.
PB  - MDPI
T2  - Plants
T1  - Interaction of carbohydrate coated cerium-oxide nanoparticles with wheat and pea: Stress induction potential and effect on development
VL  - 8
IS  - 11
DO  - 10.3390/plants8110478
ER  - 
@article{
author = "Milenković, Ivana and Mitrović, Aleksandra D. and Algarra, Manuel and Lázaro-Martínez, Juan M. and Rodríguez-Castellón, Enrique and Maksimović, Vuk and Spasić, Slađana Z. and Beškoski, Vladimir and Radotić, Ksenija",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3698",
abstract = "Reports about the influence of cerium-oxide nanoparticles (nCeO2) on plants are contradictory due to their positive and negative effects on plants. Surface modification may affect the interaction of nCeO2 with the environment, and hence its availability to plants. In this study, the uncoated and glucose-, levan-, and pullulan-coated nCeO2 were synthesized and characterized. The aim was to determine whether nontoxic carbohydrates alter the effect of nCeO2 on the seed germination, plant growth, and metabolism of wheat and pea. We applied 200 mgL-1 of nCeO2 on plants during germination (Ger treatment) or three week-growth (Gro treatment) in hydroponics. The plant response to nCeO2 was studied by measuring changes in Ce concentration, total antioxidative activity (TAA), total phenolic content (TPC), and phenolic profile. Our results generally revealed higher Ce concentration in plants after the treatment with coated nanoparticles compared to uncoated ones. Considering all obtained results, Ger treatment had a stronger impact on the later stages of plant development than Gro treatment. The Ger treatment had a stronger impact on TPC and plant elongation, whereas Gro treatment affected more TAA and phenolic profile. Among nanoparticles, levan-coated nCeO2 had the strongest and positive impact on tested plants. Wheat showed higher sensitivity to all treatments.",
publisher = "MDPI",
journal = "Plants",
title = "Interaction of carbohydrate coated cerium-oxide nanoparticles with wheat and pea: Stress induction potential and effect on development",
volume = "8",
number = "11",
doi = "10.3390/plants8110478"
}
Milenković, I., Mitrović, A. D., Algarra, M., Lázaro-Martínez, J. M., Rodríguez-Castellón, E., Maksimović, V., Spasić, S. Z., Beškoski, V.,& Radotić, K. (2019). Interaction of carbohydrate coated cerium-oxide nanoparticles with wheat and pea: Stress induction potential and effect on development.
Plants
MDPI., 8(11).
https://doi.org/10.3390/plants8110478
Milenković I, Mitrović AD, Algarra M, Lázaro-Martínez JM, Rodríguez-Castellón E, Maksimović V, Spasić SZ, Beškoski V, Radotić K. Interaction of carbohydrate coated cerium-oxide nanoparticles with wheat and pea: Stress induction potential and effect on development. Plants. 2019;8(11)
Milenković Ivana, Mitrović Aleksandra D., Algarra Manuel, Lázaro-Martínez Juan M., Rodríguez-Castellón Enrique, Maksimović Vuk, Spasić Slađana Z., Beškoski Vladimir, Radotić Ksenija, "Interaction of carbohydrate coated cerium-oxide nanoparticles with wheat and pea: Stress induction potential and effect on development" Plants, 8, no. 11 (2019),
https://doi.org/10.3390/plants8110478 .
4
1
2

Supplementary data for the article: Milenković, I.; Mitrović, A.; Algarra, M.; Lázaro-Martínez, J. M.; Rodríguez-Castellón, E.; Maksimović, V.; Spasić, S. Z.; Beškoski, V. P.; Radotić, K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. Plants 2019, 8 (11). https://doi.org/10.3390/plants8110478

Milenković, Ivana; Mitrović, Aleksandra D.; Algarra, Manuel; Lázaro-Martínez, Juan M.; Rodríguez-Castellón, Enrique; Maksimović, Vuk; Spasić, Slađana Z.; Beškoski, Vladimir; Radotić, Ksenija

(MDPI, 2019)

TY  - BOOK
AU  - Milenković, Ivana
AU  - Mitrović, Aleksandra D.
AU  - Algarra, Manuel
AU  - Lázaro-Martínez, Juan M.
AU  - Rodríguez-Castellón, Enrique
AU  - Maksimović, Vuk
AU  - Spasić, Slađana Z.
AU  - Beškoski, Vladimir
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3699
PB  - MDPI
T2  - Plants
T1  - Supplementary data for the article: Milenković, I.; Mitrović, A.; Algarra, M.; Lázaro-Martínez, J. M.; Rodríguez-Castellón, E.; Maksimović, V.; Spasić, S. Z.; Beškoski, V. P.; Radotić, K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. Plants 2019, 8 (11). https://doi.org/10.3390/plants8110478
ER  - 
@book{
author = "Milenković, Ivana and Mitrović, Aleksandra D. and Algarra, Manuel and Lázaro-Martínez, Juan M. and Rodríguez-Castellón, Enrique and Maksimović, Vuk and Spasić, Slađana Z. and Beškoski, Vladimir and Radotić, Ksenija",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3699",
publisher = "MDPI",
journal = "Plants",
title = "Supplementary data for the article: Milenković, I.; Mitrović, A.; Algarra, M.; Lázaro-Martínez, J. M.; Rodríguez-Castellón, E.; Maksimović, V.; Spasić, S. Z.; Beškoski, V. P.; Radotić, K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. Plants 2019, 8 (11). https://doi.org/10.3390/plants8110478"
}
Milenković, I., Mitrović, A. D., Algarra, M., Lázaro-Martínez, J. M., Rodríguez-Castellón, E., Maksimović, V., Spasić, S. Z., Beškoski, V.,& Radotić, K. (2019). Supplementary data for the article: Milenković, I.; Mitrović, A.; Algarra, M.; Lázaro-Martínez, J. M.; Rodríguez-Castellón, E.; Maksimović, V.; Spasić, S. Z.; Beškoski, V. P.; Radotić, K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. Plants 2019, 8 (11). https://doi.org/10.3390/plants8110478.
Plants
MDPI..
Milenković I, Mitrović AD, Algarra M, Lázaro-Martínez JM, Rodríguez-Castellón E, Maksimović V, Spasić SZ, Beškoski V, Radotić K. Supplementary data for the article: Milenković, I.; Mitrović, A.; Algarra, M.; Lázaro-Martínez, J. M.; Rodríguez-Castellón, E.; Maksimović, V.; Spasić, S. Z.; Beškoski, V. P.; Radotić, K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. Plants 2019, 8 (11). https://doi.org/10.3390/plants8110478. Plants. 2019;
Milenković Ivana, Mitrović Aleksandra D., Algarra Manuel, Lázaro-Martínez Juan M., Rodríguez-Castellón Enrique, Maksimović Vuk, Spasić Slađana Z., Beškoski Vladimir, Radotić Ksenija, "Supplementary data for the article: Milenković, I.; Mitrović, A.; Algarra, M.; Lázaro-Martínez, J. M.; Rodríguez-Castellón, E.; Maksimović, V.; Spasić, S. Z.; Beškoski, V. P.; Radotić, K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. Plants 2019, 8 (11). https://doi.org/10.3390/plants8110478" Plants (2019)

Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat

Kostić, Ljiljana; Nikolić, Nina; Samardžić, Jelena; Milisavljevic, Mira; Maksimovic, Vuk; Čakmak, Dragan; Manojlović, Dragan D.; Nikolić, Miroslav

(Springer, New York, 2015)

TY  - JOUR
AU  - Kostić, Ljiljana
AU  - Nikolić, Nina
AU  - Samardžić, Jelena
AU  - Milisavljevic, Mira
AU  - Maksimovic, Vuk
AU  - Čakmak, Dragan
AU  - Manojlović, Dragan D.
AU  - Nikolić, Miroslav
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1676
AB  - We studied the effect of liming and P fertilization of extremely acid soil (accidently acidified by sulfidic mining waste) on P availability and the subsequent adaptive responses of wheat roots. The wheat plants were grown in rhizoboxes allowing precise sampling of rhizosphere and bulk soil for sequential extraction of P fractions and determination of exchangeable Al. Root exudates were collected by pieces of paper for electrophoresis and subjected to HPLC analysis. Expression of organic anions and P-i transporter genes was analyzed by a real-time quantitative PCR. The concomitant application of lime with P fertilization increased the concentrations of plant-available P fractions in both rhizosphere and bulk compartments. The applied soil amendments strongly affected plant growth, biomass partitioning and shoot P accumulation. Liming enhanced root exudation of citrate in P unfertilized plants, while the high malate efflux was maintained until both P deficiency and Al toxicity were eliminated by the amendments. We showed the importance of liming for recovering of P acquisition potential of wheat roots, which can be strongly impaired in acid soils. Our results clearly demonstrated that P-deficient roots not subjected to Al stress in the limed soil can maintain high efflux of malate and even increase efflux of citrate along with the enhanced expression of related anion transporters (TaMATE1 and TaALMT1).
PB  - Springer, New York
T2  - Biology and Fertility of Soils
T1  - Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat
VL  - 51
IS  - 3
SP  - 289
EP  - 298
DO  - 10.1007/s00374-014-0975-y
ER  - 
@article{
author = "Kostić, Ljiljana and Nikolić, Nina and Samardžić, Jelena and Milisavljevic, Mira and Maksimovic, Vuk and Čakmak, Dragan and Manojlović, Dragan D. and Nikolić, Miroslav",
year = "2015",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1676",
abstract = "We studied the effect of liming and P fertilization of extremely acid soil (accidently acidified by sulfidic mining waste) on P availability and the subsequent adaptive responses of wheat roots. The wheat plants were grown in rhizoboxes allowing precise sampling of rhizosphere and bulk soil for sequential extraction of P fractions and determination of exchangeable Al. Root exudates were collected by pieces of paper for electrophoresis and subjected to HPLC analysis. Expression of organic anions and P-i transporter genes was analyzed by a real-time quantitative PCR. The concomitant application of lime with P fertilization increased the concentrations of plant-available P fractions in both rhizosphere and bulk compartments. The applied soil amendments strongly affected plant growth, biomass partitioning and shoot P accumulation. Liming enhanced root exudation of citrate in P unfertilized plants, while the high malate efflux was maintained until both P deficiency and Al toxicity were eliminated by the amendments. We showed the importance of liming for recovering of P acquisition potential of wheat roots, which can be strongly impaired in acid soils. Our results clearly demonstrated that P-deficient roots not subjected to Al stress in the limed soil can maintain high efflux of malate and even increase efflux of citrate along with the enhanced expression of related anion transporters (TaMATE1 and TaALMT1).",
publisher = "Springer, New York",
journal = "Biology and Fertility of Soils",
title = "Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat",
volume = "51",
number = "3",
pages = "289-298",
doi = "10.1007/s00374-014-0975-y"
}
Kostić, L., Nikolić, N., Samardžić, J., Milisavljevic, M., Maksimovic, V., Čakmak, D., Manojlović, D. D.,& Nikolić, M. (2015). Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat.
Biology and Fertility of Soils
Springer, New York., 51(3), 289-298.
https://doi.org/10.1007/s00374-014-0975-y
Kostić L, Nikolić N, Samardžić J, Milisavljevic M, Maksimovic V, Čakmak D, Manojlović DD, Nikolić M. Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat. Biology and Fertility of Soils. 2015;51(3):289-298
Kostić Ljiljana, Nikolić Nina, Samardžić Jelena, Milisavljevic Mira, Maksimovic Vuk, Čakmak Dragan, Manojlović Dragan D., Nikolić Miroslav, "Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat" Biology and Fertility of Soils, 51, no. 3 (2015):289-298,
https://doi.org/10.1007/s00374-014-0975-y .
14
11
14

Characterization of amyloglucosidase immobilized on the copolymer of ethylene glycol dimethacrylate and glycidyl methacrylate in simulated industrial conditions

Milosavić, Nenad B.; Prodanović, Radivoje; Jovanović, Slobodan M.; Maksimović, Vuk; Vujčić, Zoran

(2004)

TY  - JOUR
AU  - Milosavić, Nenad B.
AU  - Prodanović, Radivoje
AU  - Jovanović, Slobodan M.
AU  - Maksimović, Vuk
AU  - Vujčić, Zoran
PY  - 2004
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/87
AB  - The application of amyloglucosidase immobilized on the macroporous co-polymer of ethylene glycol dimethacrylate and glycidyl methacrylate (poly (GMA-co-EGDMA)) in an enzyme reactor was shown. The higher thermostability of immobilized glucoamylases than the soluble one was demonstrated. Immobilized amyloglucosidase obtained by the periodate method shows two times higher thermo stability than the soluble form. Glucoamylases immobilized on poly (GMA-co-EGDMA) have good mechanical and chemical features in the reactor and when applied in a continuous flow reactor for 28 days no changes are observed. In this period periodate immobilized amyloglucosidase shows no decrease in activity. It showed potential for the continuous production of glucose from starch over a prolonged period of time.
AB  - U okviru ovoga rada prikazani su rezultati dobijeni izučavanjem termostabilnosti dva kovalentna imobilizata amiloglukozidaze na makroporoznom poli(GMA-co-EGDMA), kao i njihova primena za hidrolizu škroba pri simuliranim industrijskim uslovima. U svim eksperimentima sa reaktorima, bilo šaržnim, bilo da se radi o reaktoru sa napakovanim slojem kao supstrat korišćen je 20 % (m/m) industrijski hidrolizat škroba. Oba imobilizata su pokazala povećanu termostabilnost u odnosu na rastvoreni enzim, ali je ona značajno veća kod imobilizata dobijenog perjodatnom metodom. Prilikom upotrebe u bač reaktoru imobilizati su deset puta brže dali krajnje DE vrednosti slične onoj koju daje rastvorni enzim. Količina i sastav šećera je po završenoj hidrolizi analiziran i HPLC-om. Određena je operativna stabilnost perjodatno imobilizovane amiloglukozidaze u reaktoru sa napakovnim slojem i pri tome je konstatovano da imobilizat ne gubi aktivnost u trajanju od 28 dana.
T2  - Hemijska industrija
T1  - Characterization of amyloglucosidase immobilized on the copolymer of ethylene glycol dimethacrylate and glycidyl methacrylate in simulated industrial conditions
T1  - Karakterizacija i primena amiloglukozidaze imobilizovane na makroporoznom polkgma-co-egdma) u simuliranim industrijskim uslovima
VL  - 58
IS  - 11
SP  - 493
EP  - 498
DO  - 10.2298/HEMIND0411493M
ER  - 
@article{
author = "Milosavić, Nenad B. and Prodanović, Radivoje and Jovanović, Slobodan M. and Maksimović, Vuk and Vujčić, Zoran",
year = "2004",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/87",
abstract = "The application of amyloglucosidase immobilized on the macroporous co-polymer of ethylene glycol dimethacrylate and glycidyl methacrylate (poly (GMA-co-EGDMA)) in an enzyme reactor was shown. The higher thermostability of immobilized glucoamylases than the soluble one was demonstrated. Immobilized amyloglucosidase obtained by the periodate method shows two times higher thermo stability than the soluble form. Glucoamylases immobilized on poly (GMA-co-EGDMA) have good mechanical and chemical features in the reactor and when applied in a continuous flow reactor for 28 days no changes are observed. In this period periodate immobilized amyloglucosidase shows no decrease in activity. It showed potential for the continuous production of glucose from starch over a prolonged period of time., U okviru ovoga rada prikazani su rezultati dobijeni izučavanjem termostabilnosti dva kovalentna imobilizata amiloglukozidaze na makroporoznom poli(GMA-co-EGDMA), kao i njihova primena za hidrolizu škroba pri simuliranim industrijskim uslovima. U svim eksperimentima sa reaktorima, bilo šaržnim, bilo da se radi o reaktoru sa napakovanim slojem kao supstrat korišćen je 20 % (m/m) industrijski hidrolizat škroba. Oba imobilizata su pokazala povećanu termostabilnost u odnosu na rastvoreni enzim, ali je ona značajno veća kod imobilizata dobijenog perjodatnom metodom. Prilikom upotrebe u bač reaktoru imobilizati su deset puta brže dali krajnje DE vrednosti slične onoj koju daje rastvorni enzim. Količina i sastav šećera je po završenoj hidrolizi analiziran i HPLC-om. Određena je operativna stabilnost perjodatno imobilizovane amiloglukozidaze u reaktoru sa napakovnim slojem i pri tome je konstatovano da imobilizat ne gubi aktivnost u trajanju od 28 dana.",
journal = "Hemijska industrija",
title = "Characterization of amyloglucosidase immobilized on the copolymer of ethylene glycol dimethacrylate and glycidyl methacrylate in simulated industrial conditions, Karakterizacija i primena amiloglukozidaze imobilizovane na makroporoznom polkgma-co-egdma) u simuliranim industrijskim uslovima",
volume = "58",
number = "11",
pages = "493-498",
doi = "10.2298/HEMIND0411493M"
}
Milosavić, N. B., Prodanović, R., Jovanović, S. M., Maksimović, V.,& Vujčić, Z. (2004). Karakterizacija i primena amiloglukozidaze imobilizovane na makroporoznom polkgma-co-egdma) u simuliranim industrijskim uslovima.
Hemijska industrija, 58(11), 493-498.
https://doi.org/10.2298/HEMIND0411493M
Milosavić NB, Prodanović R, Jovanović SM, Maksimović V, Vujčić Z. Karakterizacija i primena amiloglukozidaze imobilizovane na makroporoznom polkgma-co-egdma) u simuliranim industrijskim uslovima. Hemijska industrija. 2004;58(11):493-498
Milosavić Nenad B., Prodanović Radivoje, Jovanović Slobodan M., Maksimović Vuk, Vujčić Zoran, "Karakterizacija i primena amiloglukozidaze imobilizovane na makroporoznom polkgma-co-egdma) u simuliranim industrijskim uslovima" Hemijska industrija, 58, no. 11 (2004):493-498,
https://doi.org/10.2298/HEMIND0411493M .
1