TY  - JOUR
AU  - Galić, Milica
AU  - Ćilerdžić, Jasmina
AU  - Vukojević, Jelena
AU  - Stajić, Mirjana
AU  - Brčeski, Ilija
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/357
AB  - For the last several years, there is an increasing research interest for lignocellulosic biomass because of its renewable nature. Wheat straw, as one of the most abundant plant wastes, could potentially be transformed into various high valued products (food, feed, paper, biofuel). Micromycetes play an important role in lignocellulose conversion owing to their powerfull enzymatic system. They posses different potentials to synthetise lignocelullolytic enzymes and degrade biomass. Therefore, the objectives of this study were determination of Mn-oxidising peroxidases and laccases activities and extent of wheat straw depolymerisation during solid-state fermentation by selected micromycetes species/strains. The highest Mn-dependent-and Mn-independent peroxidases activities were noted in Clonostachys rosea BEOFB 1610m on day 14 of cultivation (210.23 and 303.03 U l–1, respectively), while Alternaria sp. BEOFB 202m was the unique producer of laccase which maximum activity (1558.59 U l–1) was observed after 7 days of wheat straw fermentation. After 21 days of wheat straw depolymerisation, the highest level of lignin degradation was caused by C. rosea BEOFB 1610m (13.67%), while Alternaria sp. BEOFB 202m was the most efficient hemicellulose and cellulose degrader (38.1 and 29.8%, respectively). These data demonstrate that studied fungal species/strains could potentially be used in various biotechnological processes for the plant raw materials transformation. © 2018, Scibulcom Ltd. All rights reserved.
T2  - Journal of Environmental Protection and Ecology
T1  - Potential of selected micromycetes for wheat straw degradation
VL  - 19
IS  - 3
SP  - 1116
EP  - 1122
UR  - https://hdl.handle.net/21.15107/rcub_cherry_357
ER  - 

@article{
author = "Galić, Milica and Ćilerdžić, Jasmina and Vukojević, Jelena and Stajić, Mirjana and Brčeski, Ilija",
year = "2018",
abstract = "For the last several years, there is an increasing research interest for lignocellulosic biomass because of its renewable nature. Wheat straw, as one of the most abundant plant wastes, could potentially be transformed into various high valued products (food, feed, paper, biofuel). Micromycetes play an important role in lignocellulose conversion owing to their powerfull enzymatic system. They posses different potentials to synthetise lignocelullolytic enzymes and degrade biomass. Therefore, the objectives of this study were determination of Mn-oxidising peroxidases and laccases activities and extent of wheat straw depolymerisation during solid-state fermentation by selected micromycetes species/strains. The highest Mn-dependent-and Mn-independent peroxidases activities were noted in Clonostachys rosea BEOFB 1610m on day 14 of cultivation (210.23 and 303.03 U l–1, respectively), while Alternaria sp. BEOFB 202m was the unique producer of laccase which maximum activity (1558.59 U l–1) was observed after 7 days of wheat straw fermentation. After 21 days of wheat straw depolymerisation, the highest level of lignin degradation was caused by C. rosea BEOFB 1610m (13.67%), while Alternaria sp. BEOFB 202m was the most efficient hemicellulose and cellulose degrader (38.1 and 29.8%, respectively). These data demonstrate that studied fungal species/strains could potentially be used in various biotechnological processes for the plant raw materials transformation. © 2018, Scibulcom Ltd. All rights reserved.",
journal = "Journal of Environmental Protection and Ecology",
title = "Potential of selected micromycetes for wheat straw degradation",
volume = "19",
number = "3",
pages = "1116-1122",
url = "https://hdl.handle.net/21.15107/rcub_cherry_357"
}

Galić, M., Ćilerdžić, J., Vukojević, J., Stajić, M.,& Brčeski, I.. (2018). Potential of selected micromycetes for wheat straw degradation. in Journal of Environmental Protection and Ecology, 19(3), 1116-1122.
https://hdl.handle.net/21.15107/rcub_cherry_357

Galić M, Ćilerdžić J, Vukojević J, Stajić M, Brčeski I. Potential of selected micromycetes for wheat straw degradation. in Journal of Environmental Protection and Ecology. 2018;19(3):1116-1122.
https://hdl.handle.net/21.15107/rcub_cherry_357 .

Galić, Milica, Ćilerdžić, Jasmina, Vukojević, Jelena, Stajić, Mirjana, Brčeski, Ilija, "Potential of selected micromycetes for wheat straw degradation" in Journal of Environmental Protection and Ecology, 19, no. 3 (2018):1116-1122,
https://hdl.handle.net/21.15107/rcub_cherry_357 .

TY  - JOUR
AU  - Ćilerdžić, Jasmina
AU  - Galić, Milica
AU  - Ivanović, Žarko
AU  - Brčeski, Ilija
AU  - Vukojević, Jelena
AU  - Stajić, Mirjana
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/346
AB  - Biological pretreatment of the lignocellulosic residues, in which white-rot fungi have a crucial role, has many advantages compared to the chemical, physical, and physico-chemical methods of delignification and therefore attracts increasing scientific attention. Regarding the fact that properties and capacities of the ligninolytic enzymes of Daedaleopsis spp. are still unknown, the aim of this study was to research how nitrogen sources and inducers affect the potential of Daedaleopsis confragosa and Daedaleopsis tricolor to degrade cherry sawdust. NH4NO3, (NH4)2SO4, and peptone were tested as nitrogen sources, while veratryl alcohol, p-anisidine, vanillic acid, and phenylmethylsulfonyl fluoride were the studied inducers. As Mn-dependent peroxidase and laccase were the leader enzymes and cherry sawdust/peptone medium the best stimulator of their activities, the effect of inducers on delignification potential of these species was studied during fermentation of that substrate. Veratryl alcohol was the best stimulator of laccase and phenylmethylsulfonyl fluoride of Mn-dependent peroxidase activity (27,610.0 and 1338.4 U/L, respectively). These inducers also increased cherry sawdust delignification selectivity, particularly in D. tricolor in the presence of phenylmethylsulfonyl fluoride (lignin:hemicellulose:cellulose = 32.1%:0.9%:11.7%). Owing to the presented results, studied species could have an important role in the phase of lignocellulose pretreatment in various biotechnological processes. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Applied Biochemistry and Biotechnology
T1  - Stimulation of Wood Degradation by Daedaleopsis confragosa and D. tricolor
DO  - 10.1007/s12010-018-2884-2
ER  - 

@article{
author = "Ćilerdžić, Jasmina and Galić, Milica and Ivanović, Žarko and Brčeski, Ilija and Vukojević, Jelena and Stajić, Mirjana",
year = "2018",
abstract = "Biological pretreatment of the lignocellulosic residues, in which white-rot fungi have a crucial role, has many advantages compared to the chemical, physical, and physico-chemical methods of delignification and therefore attracts increasing scientific attention. Regarding the fact that properties and capacities of the ligninolytic enzymes of Daedaleopsis spp. are still unknown, the aim of this study was to research how nitrogen sources and inducers affect the potential of Daedaleopsis confragosa and Daedaleopsis tricolor to degrade cherry sawdust. NH4NO3, (NH4)2SO4, and peptone were tested as nitrogen sources, while veratryl alcohol, p-anisidine, vanillic acid, and phenylmethylsulfonyl fluoride were the studied inducers. As Mn-dependent peroxidase and laccase were the leader enzymes and cherry sawdust/peptone medium the best stimulator of their activities, the effect of inducers on delignification potential of these species was studied during fermentation of that substrate. Veratryl alcohol was the best stimulator of laccase and phenylmethylsulfonyl fluoride of Mn-dependent peroxidase activity (27,610.0 and 1338.4 U/L, respectively). These inducers also increased cherry sawdust delignification selectivity, particularly in D. tricolor in the presence of phenylmethylsulfonyl fluoride (lignin:hemicellulose:cellulose = 32.1%:0.9%:11.7%). Owing to the presented results, studied species could have an important role in the phase of lignocellulose pretreatment in various biotechnological processes. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Applied Biochemistry and Biotechnology",
title = "Stimulation of Wood Degradation by Daedaleopsis confragosa and D. tricolor",
doi = "10.1007/s12010-018-2884-2"
}

Ćilerdžić, J., Galić, M., Ivanović, Ž., Brčeski, I., Vukojević, J.,& Stajić, M.. (2018). Stimulation of Wood Degradation by Daedaleopsis confragosa and D. tricolor. in Applied Biochemistry and Biotechnology.
https://doi.org/10.1007/s12010-018-2884-2

Ćilerdžić J, Galić M, Ivanović Ž, Brčeski I, Vukojević J, Stajić M. Stimulation of Wood Degradation by Daedaleopsis confragosa and D. tricolor. in Applied Biochemistry and Biotechnology. 2018;.
doi:10.1007/s12010-018-2884-2 .

Ćilerdžić, Jasmina, Galić, Milica, Ivanović, Žarko, Brčeski, Ilija, Vukojević, Jelena, Stajić, Mirjana, "Stimulation of Wood Degradation by Daedaleopsis confragosa and D. tricolor" in Applied Biochemistry and Biotechnology (2018),
https://doi.org/10.1007/s12010-018-2884-2 . .

TY  - JOUR
AU  - Ćilerdžić, Jasmina
AU  - Galić, Milica
AU  - Vukojević, Jelena
AU  - Brčeski, Ilija
AU  - Stajić, Mirjana
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/321
AB  - Background: Structural component of plant biomass, lignocellulose, is the most abundant renewable resource in nature. Lignin is the most recalcitrant natural aromatic polymer and its degradation presents great challenge. Nowadays, the special attention is given to biological delignification, the process where white-rot fungi take the crucial place owing to strong ligninolytic enzyme system. However, fungal species, even strains, differ in potential to produce high active ligninolytic enzymes and consequently to delignify plant biomass. Therefore, the goals of the study were characterization of Mn-oxidizing peroxidases and laccases of numerous mushrooms as well as determination of their potential to delignify wheat straw, the plant raw material that, according to annual yield, takes the first place in Europe and the second one in the world. Results: During wheat straw fermentation, Lentinus edodes HAI 858 produced the most active Mn-dependent and Mn-independent peroxidases (1443.2 U L-1 and 1045.5 U L-1, respectively), while Pleurotus eryngii HAI 711 was the best laccase producer (7804.3 U L-1). Visualized bends on zymogram confirmed these activities and demonstrated that laccases were the dominant ligninolytic enzymes in the studied species. Ganoderma lucidum BEOFB 435 showed considerable ability to degrade lignin (58.5%) and especially hemicellulose (74.8%), while the cellulose remained almost intact (0.7%). Remarkable selectivity in lignocellulose degradation was also noted in Pleurotus pulmonarius HAI 573 where degraded amounts of lignin, hemicellulose and cellulose were in ratio of 50.4%:15.3%:3.8%. Conclusions: According to the presented results, it can be concluded that white-rot fungi, due to ligninolytic enzymes features and degradation potential, could be important participants in various biotechnological processes including biotransformation of lignocellulose residues/wastes in food, feed, paper and biofuels. © 2017 The Author(s).
T2  - BMC Plant Biology
T1  - Potential of selected fungal species to degrade wheat straw, the most abundant plant raw material in Europe
VL  - 17
DO  - 10.1186/s12870-017-1196-y
ER  - 

@article{
author = "Ćilerdžić, Jasmina and Galić, Milica and Vukojević, Jelena and Brčeski, Ilija and Stajić, Mirjana",
year = "2017",
abstract = "Background: Structural component of plant biomass, lignocellulose, is the most abundant renewable resource in nature. Lignin is the most recalcitrant natural aromatic polymer and its degradation presents great challenge. Nowadays, the special attention is given to biological delignification, the process where white-rot fungi take the crucial place owing to strong ligninolytic enzyme system. However, fungal species, even strains, differ in potential to produce high active ligninolytic enzymes and consequently to delignify plant biomass. Therefore, the goals of the study were characterization of Mn-oxidizing peroxidases and laccases of numerous mushrooms as well as determination of their potential to delignify wheat straw, the plant raw material that, according to annual yield, takes the first place in Europe and the second one in the world. Results: During wheat straw fermentation, Lentinus edodes HAI 858 produced the most active Mn-dependent and Mn-independent peroxidases (1443.2 U L-1 and 1045.5 U L-1, respectively), while Pleurotus eryngii HAI 711 was the best laccase producer (7804.3 U L-1). Visualized bends on zymogram confirmed these activities and demonstrated that laccases were the dominant ligninolytic enzymes in the studied species. Ganoderma lucidum BEOFB 435 showed considerable ability to degrade lignin (58.5%) and especially hemicellulose (74.8%), while the cellulose remained almost intact (0.7%). Remarkable selectivity in lignocellulose degradation was also noted in Pleurotus pulmonarius HAI 573 where degraded amounts of lignin, hemicellulose and cellulose were in ratio of 50.4%:15.3%:3.8%. Conclusions: According to the presented results, it can be concluded that white-rot fungi, due to ligninolytic enzymes features and degradation potential, could be important participants in various biotechnological processes including biotransformation of lignocellulose residues/wastes in food, feed, paper and biofuels. © 2017 The Author(s).",
journal = "BMC Plant Biology",
title = "Potential of selected fungal species to degrade wheat straw, the most abundant plant raw material in Europe",
volume = "17",
doi = "10.1186/s12870-017-1196-y"
}

Ćilerdžić, J., Galić, M., Vukojević, J., Brčeski, I.,& Stajić, M.. (2017). Potential of selected fungal species to degrade wheat straw, the most abundant plant raw material in Europe. in BMC Plant Biology, 17.
https://doi.org/10.1186/s12870-017-1196-y

Ćilerdžić J, Galić M, Vukojević J, Brčeski I, Stajić M. Potential of selected fungal species to degrade wheat straw, the most abundant plant raw material in Europe. in BMC Plant Biology. 2017;17.
doi:10.1186/s12870-017-1196-y .

Ćilerdžić, Jasmina, Galić, Milica, Vukojević, Jelena, Brčeski, Ilija, Stajić, Mirjana, "Potential of selected fungal species to degrade wheat straw, the most abundant plant raw material in Europe" in BMC Plant Biology, 17 (2017),
https://doi.org/10.1186/s12870-017-1196-y . .