TY  - JOUR
AU  - Đekić, Ilija
AU  - Lević, Steva
AU  - Šmigić, Nada
AU  - Bouleau, Arthur
AU  - Ilijević, Konstantin
AU  - Roganović, Jovana
AU  - Rakić, Vesna
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6459
AB  - Titanium dioxide (TiO2) is banned in some countries but its use is still permitted in others. The global food supply chain is therefore challenged with the need to use rapid and reliable testing methods to either detect the presence of TiO2 or to quantify its concentration. The goal of this study was to determine the feasibility of using color, texture profile analysis, Raman microscopy, and X-ray fluorescence (XRF) spectroscopy to detect and quantify TiO2 in fillings used in the pastry and confectionery industry. In this study, two types of fillings were investigated: vanilla based and chocolate based. All fillings were prepared in four different variations – without TiO2 and with three concentrations as follows: 0.25 g*kg-1, 0.5 g*kg-1, or 0.75 g*kg-1 TiO2 per sample. The methods were selected for their ability to analyze the samples in a short period of time. All of the methods showed moderate to high potential for detecting TiO2 in the samples. The results reveal how TiO2 affects the food matrix color and texture. Use of Raman microscopy confirms its detectability, although concentrations of TiO2 do not follow a pattern. X-ray fluorescence spectroscopy showed the greatest potential as it can not only detect TiO2 but can also quantify its concentration in the samples. The highest potential for quantifying the concentration of this food additive was achieved with XRF.
T2  - Journal of the Science of Food and Agriculture
T1  - Challenges and potential for detecting and quantifying titanium dioxide in food
VL  - n/a
DO  - 10.1002/jsfa.13356
ER  - 

@article{
author = "Đekić, Ilija and Lević, Steva and Šmigić, Nada and Bouleau, Arthur and Ilijević, Konstantin and Roganović, Jovana and Rakić, Vesna",
year = "2024",
abstract = "Titanium dioxide (TiO2) is banned in some countries but its use is still permitted in others. The global food supply chain is therefore challenged with the need to use rapid and reliable testing methods to either detect the presence of TiO2 or to quantify its concentration. The goal of this study was to determine the feasibility of using color, texture profile analysis, Raman microscopy, and X-ray fluorescence (XRF) spectroscopy to detect and quantify TiO2 in fillings used in the pastry and confectionery industry. In this study, two types of fillings were investigated: vanilla based and chocolate based. All fillings were prepared in four different variations – without TiO2 and with three concentrations as follows: 0.25 g*kg-1, 0.5 g*kg-1, or 0.75 g*kg-1 TiO2 per sample. The methods were selected for their ability to analyze the samples in a short period of time. All of the methods showed moderate to high potential for detecting TiO2 in the samples. The results reveal how TiO2 affects the food matrix color and texture. Use of Raman microscopy confirms its detectability, although concentrations of TiO2 do not follow a pattern. X-ray fluorescence spectroscopy showed the greatest potential as it can not only detect TiO2 but can also quantify its concentration in the samples. The highest potential for quantifying the concentration of this food additive was achieved with XRF.",
journal = "Journal of the Science of Food and Agriculture",
title = "Challenges and potential for detecting and quantifying titanium dioxide in food",
volume = "n/a",
doi = "10.1002/jsfa.13356"
}

Đekić, I., Lević, S., Šmigić, N., Bouleau, A., Ilijević, K., Roganović, J.,& Rakić, V.. (2024). Challenges and potential for detecting and quantifying titanium dioxide in food. in Journal of the Science of Food and Agriculture, n/a.
https://doi.org/10.1002/jsfa.13356

Đekić I, Lević S, Šmigić N, Bouleau A, Ilijević K, Roganović J, Rakić V. Challenges and potential for detecting and quantifying titanium dioxide in food. in Journal of the Science of Food and Agriculture. 2024;n/a.
doi:10.1002/jsfa.13356 .

Đekić, Ilija, Lević, Steva, Šmigić, Nada, Bouleau, Arthur, Ilijević, Konstantin, Roganović, Jovana, Rakić, Vesna, "Challenges and potential for detecting and quantifying titanium dioxide in food" in Journal of the Science of Food and Agriculture, n/a (2024),
https://doi.org/10.1002/jsfa.13356 . .

TY  - JOUR
AU  - Orlić, Jovana
AU  - Aničić-Urošević, Mira
AU  - Vergel, Konstantin
AU  - Zinicovscaia, Inga
AU  - Stojadinović, Sanja M.
AU  - Gržetić, Ivan
AU  - Ilijević, Konstantin
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5007
AB  - Conventionally used spectrometric techniques of inductively coupled plasma optical emission spectrometry (ICP-OES) and inductively coupled plasma optical emission spectrometry (ICP-MS) usually involve time-consuming sample preparation procedure of a sample dissolution which requires the usage of aggressive and toxic chemicals. The need for suitable and sustainable analytical methods for direct multi-elemental analysis of plant samples has been increased in recent years. Spectrometric techniques for direct sample analysis, instrumental neutron activation analysis (INAA) and X-ray fluorescence (XRF) have been applied in environmental studies and various fields of screening tests. Nevertheless, these techniques are not commonly used for plant sample analysis and their performances need to be evaluated. This research aimed to assess how reliable non-destructive techniques are in the determination of elements in plants compared to conventionally used spectrometric techniques. A total of 49 plant samples of four conifer species (Pinus nigra, Abies alba, Taxus baccata and Larix decidua) were measured using two conventionally applied (ICP-MS, ICP-OES) and two non-destructive techniques (wavelength dispersive XRF (WD-XRF), INAA). The comparison was performed by investigation of relative ratios of concentrations and by correlation analysis. Moreover, precision of the techniques was examined and compared. The quality control included analysis of NIST pine needles certified reference material (1575a) using all examined techniques. Our results suggest that additional analytical and quality control steps are necessary for reaching the highest accuracy of multi-elemental analysis.
T2  - Journal of the Serbian Chemical Society
T1  - Comparison of non-destructive techniques and conventionally used spectrometric techniques for determination of elements in plant samples (coniferous leaves)
VL  - 87
IS  - 1
SP  - 69
EP  - 81
DO  - 10.2298/JSC210921101O
ER  - 

@article{
author = "Orlić, Jovana and Aničić-Urošević, Mira and Vergel, Konstantin and Zinicovscaia, Inga and Stojadinović, Sanja M. and Gržetić, Ivan and Ilijević, Konstantin",
year = "2022",
abstract = "Conventionally used spectrometric techniques of inductively coupled plasma optical emission spectrometry (ICP-OES) and inductively coupled plasma optical emission spectrometry (ICP-MS) usually involve time-consuming sample preparation procedure of a sample dissolution which requires the usage of aggressive and toxic chemicals. The need for suitable and sustainable analytical methods for direct multi-elemental analysis of plant samples has been increased in recent years. Spectrometric techniques for direct sample analysis, instrumental neutron activation analysis (INAA) and X-ray fluorescence (XRF) have been applied in environmental studies and various fields of screening tests. Nevertheless, these techniques are not commonly used for plant sample analysis and their performances need to be evaluated. This research aimed to assess how reliable non-destructive techniques are in the determination of elements in plants compared to conventionally used spectrometric techniques. A total of 49 plant samples of four conifer species (Pinus nigra, Abies alba, Taxus baccata and Larix decidua) were measured using two conventionally applied (ICP-MS, ICP-OES) and two non-destructive techniques (wavelength dispersive XRF (WD-XRF), INAA). The comparison was performed by investigation of relative ratios of concentrations and by correlation analysis. Moreover, precision of the techniques was examined and compared. The quality control included analysis of NIST pine needles certified reference material (1575a) using all examined techniques. Our results suggest that additional analytical and quality control steps are necessary for reaching the highest accuracy of multi-elemental analysis.",
journal = "Journal of the Serbian Chemical Society",
title = "Comparison of non-destructive techniques and conventionally used spectrometric techniques for determination of elements in plant samples (coniferous leaves)",
volume = "87",
number = "1",
pages = "69-81",
doi = "10.2298/JSC210921101O"
}

Orlić, J., Aničić-Urošević, M., Vergel, K., Zinicovscaia, I., Stojadinović, S. M., Gržetić, I.,& Ilijević, K.. (2022). Comparison of non-destructive techniques and conventionally used spectrometric techniques for determination of elements in plant samples (coniferous leaves). in Journal of the Serbian Chemical Society, 87(1), 69-81.
https://doi.org/10.2298/JSC210921101O

Orlić J, Aničić-Urošević M, Vergel K, Zinicovscaia I, Stojadinović SM, Gržetić I, Ilijević K. Comparison of non-destructive techniques and conventionally used spectrometric techniques for determination of elements in plant samples (coniferous leaves). in Journal of the Serbian Chemical Society. 2022;87(1):69-81.
doi:10.2298/JSC210921101O .

Orlić, Jovana, Aničić-Urošević, Mira, Vergel, Konstantin, Zinicovscaia, Inga, Stojadinović, Sanja M., Gržetić, Ivan, Ilijević, Konstantin, "Comparison of non-destructive techniques and conventionally used spectrometric techniques for determination of elements in plant samples (coniferous leaves)" in Journal of the Serbian Chemical Society, 87, no. 1 (2022):69-81,
https://doi.org/10.2298/JSC210921101O . .

TY  - CONF
AU  - Orlić, Jovana
AU  - Aničić Urošević, M.
AU  - Vergel, K.
AU  - Zinicovscaia, I.
AU  - Stojadinović, Sanja M.
AU  - Ilijević, Konstantin
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4912
AB  - The elemental composition of plant matrices has been conventionally determined by spectrometric techniques such as Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES), and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) [1]. Wet mineralization (digestion) of samples requires time, equipment, and usage of aggressive and toxic chemicals which are the main drawbacks of those routinely used techniques [2]. The need for suitable analytical methods for direct and multi-elemental analysis of plant samples has been increased in recent years [3]. Instrumental Neutron Activation Analysis (INAA) is one of the techniques for direct analysis which has been previously applied in environmental studies, nevertheless it is not a commonly used technique for plant samples. X-ray fluorescence (XRF) is another technique with the possibility of performing multi-element analysis directly on solid samples with numerous advantages. Although non-destructive techniques (INAA and XRF) are widely accepted in various fields of screening tests regarding the analytical approach, their performance needs to be evaluated in plant sample analysis. The main aim of this research was to assess how reliable non-destructive techniques are in detecting elements in conifer needles regarding routinely used spectrometric techniques. A total of 49 plant samples of four conifer species (Pinus nigra, Abies alba, Taxus baccata, and Larix decidua) were measured using two routinely used (ICPMS and ICP-OES) and two non-destructive instrumental techniques (WD-XRF and INAA). A quality control program included NIST pine needles certified reference material (1575a) analysis using all examined techniques. The techniques were compared by examination of relative ratio (element concentration measured using investigated analytical techniques divided by concentration determined by ICP-MS (figure 1)) and by correlation. Precision of all examined techniques was additionally investigated. This study confirmed that non-destructive spectroscopic techniques can be successfully applied on plant samples since sample preparation for these techniques is fast and in good accordance with the principles of green chemistry. Investigated standardless XRF method can also produce well-correlated results, compared to other techniques based on calibration standards. Obtained results suggest that the high accuracy of the analysis can be ensured by additional analytical and quality control steps (the use of internal standards, standard addition, etc.).
PB  - Belgrade : Serbian Chemical Society
C3  - Book of Abstracts 21st; European Meeting on Environmental Chemistry
T1  - Comparison of ICP-MS, ICP-OES, INAA, and WDXRF Techniques in Measuring Elements in Coniferous Needles Samples
SP  - 92
EP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4912
ER  - 

@conference{
author = "Orlić, Jovana and Aničić Urošević, M. and Vergel, K. and Zinicovscaia, I. and Stojadinović, Sanja M. and Ilijević, Konstantin",
year = "2021",
abstract = "The elemental composition of plant matrices has been conventionally determined by spectrometric techniques such as Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES), and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) [1]. Wet mineralization (digestion) of samples requires time, equipment, and usage of aggressive and toxic chemicals which are the main drawbacks of those routinely used techniques [2]. The need for suitable analytical methods for direct and multi-elemental analysis of plant samples has been increased in recent years [3]. Instrumental Neutron Activation Analysis (INAA) is one of the techniques for direct analysis which has been previously applied in environmental studies, nevertheless it is not a commonly used technique for plant samples. X-ray fluorescence (XRF) is another technique with the possibility of performing multi-element analysis directly on solid samples with numerous advantages. Although non-destructive techniques (INAA and XRF) are widely accepted in various fields of screening tests regarding the analytical approach, their performance needs to be evaluated in plant sample analysis. The main aim of this research was to assess how reliable non-destructive techniques are in detecting elements in conifer needles regarding routinely used spectrometric techniques. A total of 49 plant samples of four conifer species (Pinus nigra, Abies alba, Taxus baccata, and Larix decidua) were measured using two routinely used (ICPMS and ICP-OES) and two non-destructive instrumental techniques (WD-XRF and INAA). A quality control program included NIST pine needles certified reference material (1575a) analysis using all examined techniques. The techniques were compared by examination of relative ratio (element concentration measured using investigated analytical techniques divided by concentration determined by ICP-MS (figure 1)) and by correlation. Precision of all examined techniques was additionally investigated. This study confirmed that non-destructive spectroscopic techniques can be successfully applied on plant samples since sample preparation for these techniques is fast and in good accordance with the principles of green chemistry. Investigated standardless XRF method can also produce well-correlated results, compared to other techniques based on calibration standards. Obtained results suggest that the high accuracy of the analysis can be ensured by additional analytical and quality control steps (the use of internal standards, standard addition, etc.).",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Book of Abstracts 21st; European Meeting on Environmental Chemistry",
title = "Comparison of ICP-MS, ICP-OES, INAA, and WDXRF Techniques in Measuring Elements in Coniferous Needles Samples",
pages = "92-92",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4912"
}

Orlić, J., Aničić Urošević, M., Vergel, K., Zinicovscaia, I., Stojadinović, S. M.,& Ilijević, K.. (2021). Comparison of ICP-MS, ICP-OES, INAA, and WDXRF Techniques in Measuring Elements in Coniferous Needles Samples. in Book of Abstracts 21st; European Meeting on Environmental Chemistry
Belgrade : Serbian Chemical Society., 92-92.
https://hdl.handle.net/21.15107/rcub_cherry_4912

Orlić J, Aničić Urošević M, Vergel K, Zinicovscaia I, Stojadinović SM, Ilijević K. Comparison of ICP-MS, ICP-OES, INAA, and WDXRF Techniques in Measuring Elements in Coniferous Needles Samples. in Book of Abstracts 21st; European Meeting on Environmental Chemistry. 2021;:92-92.
https://hdl.handle.net/21.15107/rcub_cherry_4912 .

Orlić, Jovana, Aničić Urošević, M., Vergel, K., Zinicovscaia, I., Stojadinović, Sanja M., Ilijević, Konstantin, "Comparison of ICP-MS, ICP-OES, INAA, and WDXRF Techniques in Measuring Elements in Coniferous Needles Samples" in Book of Abstracts 21st; European Meeting on Environmental Chemistry (2021):92-92,
https://hdl.handle.net/21.15107/rcub_cherry_4912 .

TY  - JOUR
AU  - Orlić, Jovana
AU  - Gržetić, Ivan
AU  - Goessler, Walter
AU  - Braeuer, Simone
AU  - Čáslavský, Josef
AU  - Pořízka, Jaromír
AU  - Ilijević, Konstantin
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0168583X21002238
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4614
AB  - This research explores the possibilities and limitations of WD-XRF, applied as a method for quantification of 20 elements in plant material, using spiked cellulose standards for calibration. Three different analytical methods were investigated: 1) standards created from pure spiked cellulose; 2) spiked cellulose mixed with 20% of binder and 3) spiked cellulose applied as a thin layer on an inert carrier. Sensitivity, linearity, limit of detection, limit of quantification, repeatability, intralaboratory reproducibility, and accuracy were determined and compared. The accuracy of the investigated methods was tested by analysis of standard reference materials and comparison with other routinely used analytical techniques (ICP-OES and ICP-MS). The comparison included real plant samples which were collected from the environment characterized by different pollution levels. The accuracy of the semiquantitative standardless method was also considered and compared with other investigated methods. Tested methods can be very precise, with good intralaboratory reproducibility over wide linear range.
PB  - Elsevier
T2  - Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
T1  - Artificial cellulose standards as calibration standards for wavelength-dispersive X-ray fluorescence analysis of elements in plant samples
VL  - 502
SP  - 106
EP  - 117
DO  - 10.1016/j.nimb.2021.06.012
ER  - 

@article{
author = "Orlić, Jovana and Gržetić, Ivan and Goessler, Walter and Braeuer, Simone and Čáslavský, Josef and Pořízka, Jaromír and Ilijević, Konstantin",
year = "2021",
abstract = "This research explores the possibilities and limitations of WD-XRF, applied as a method for quantification of 20 elements in plant material, using spiked cellulose standards for calibration. Three different analytical methods were investigated: 1) standards created from pure spiked cellulose; 2) spiked cellulose mixed with 20% of binder and 3) spiked cellulose applied as a thin layer on an inert carrier. Sensitivity, linearity, limit of detection, limit of quantification, repeatability, intralaboratory reproducibility, and accuracy were determined and compared. The accuracy of the investigated methods was tested by analysis of standard reference materials and comparison with other routinely used analytical techniques (ICP-OES and ICP-MS). The comparison included real plant samples which were collected from the environment characterized by different pollution levels. The accuracy of the semiquantitative standardless method was also considered and compared with other investigated methods. Tested methods can be very precise, with good intralaboratory reproducibility over wide linear range.",
publisher = "Elsevier",
journal = "Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms",
title = "Artificial cellulose standards as calibration standards for wavelength-dispersive X-ray fluorescence analysis of elements in plant samples",
volume = "502",
pages = "106-117",
doi = "10.1016/j.nimb.2021.06.012"
}

Orlić, J., Gržetić, I., Goessler, W., Braeuer, S., Čáslavský, J., Pořízka, J.,& Ilijević, K.. (2021). Artificial cellulose standards as calibration standards for wavelength-dispersive X-ray fluorescence analysis of elements in plant samples. in Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
Elsevier., 502, 106-117.
https://doi.org/10.1016/j.nimb.2021.06.012

Orlić J, Gržetić I, Goessler W, Braeuer S, Čáslavský J, Pořízka J, Ilijević K. Artificial cellulose standards as calibration standards for wavelength-dispersive X-ray fluorescence analysis of elements in plant samples. in Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. 2021;502:106-117.
doi:10.1016/j.nimb.2021.06.012 .

Orlić, Jovana, Gržetić, Ivan, Goessler, Walter, Braeuer, Simone, Čáslavský, Josef, Pořízka, Jaromír, Ilijević, Konstantin, "Artificial cellulose standards as calibration standards for wavelength-dispersive X-ray fluorescence analysis of elements in plant samples" in Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 502 (2021):106-117,
https://doi.org/10.1016/j.nimb.2021.06.012 . .

TY  - DATA
AU  - Orlić, Jovana
AU  - Gržetić, Ivan
AU  - Goessler, Walter
AU  - Braeuer, Simone
AU  - Čáslavský, Josef
AU  - Pořízka, Jaromír
AU  - Ilijević, Konstantin
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0168583X21002238
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4615
AB  - This research explores the possibilities and limitations of WD-XRF, applied as a method for quantification of 20 elements in plant material, using spiked cellulose standards for calibration. Three different analytical methods were investigated: 1) standards created from pure spiked cellulose; 2) spiked cellulose mixed with 20% of binder and 3) spiked cellulose applied as a thin layer on an inert carrier. Sensitivity, linearity, limit of detection, limit of quantification, repeatability, intralaboratory reproducibility, and accuracy were determined and compared. The accuracy of the investigated methods was tested by analysis of standard reference materials and comparison with other routinely used analytical techniques (ICP-OES and ICP-MS). The comparison included real plant samples which were collected from the environment characterized by different pollution levels. The accuracy of the semiquantitative standardless method was also considered and compared with other investigated methods. Tested methods can be very precise, with good intralaboratory reproducibility over wide linear range.
PB  - Elsevier
T2  - Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
T1  - Supplementary data for the article: Orlić, J.; Gržetić, I.; Goessler, W.; Braeuer, S.; Čáslavský, J.; Pořízka, J.; Ilijević, K. Artificial Cellulose Standards as Calibration Standards for Wavelength-Dispersive X-Ray Fluorescence Analysis of Elements in Plant Samples. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 2021, 502, 106–117. https://doi.org/10.1016/j.nimb.2021.06.012.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4615
ER  - 

@misc{
author = "Orlić, Jovana and Gržetić, Ivan and Goessler, Walter and Braeuer, Simone and Čáslavský, Josef and Pořízka, Jaromír and Ilijević, Konstantin",
year = "2021",
abstract = "This research explores the possibilities and limitations of WD-XRF, applied as a method for quantification of 20 elements in plant material, using spiked cellulose standards for calibration. Three different analytical methods were investigated: 1) standards created from pure spiked cellulose; 2) spiked cellulose mixed with 20% of binder and 3) spiked cellulose applied as a thin layer on an inert carrier. Sensitivity, linearity, limit of detection, limit of quantification, repeatability, intralaboratory reproducibility, and accuracy were determined and compared. The accuracy of the investigated methods was tested by analysis of standard reference materials and comparison with other routinely used analytical techniques (ICP-OES and ICP-MS). The comparison included real plant samples which were collected from the environment characterized by different pollution levels. The accuracy of the semiquantitative standardless method was also considered and compared with other investigated methods. Tested methods can be very precise, with good intralaboratory reproducibility over wide linear range.",
publisher = "Elsevier",
journal = "Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms",
title = "Supplementary data for the article: Orlić, J.; Gržetić, I.; Goessler, W.; Braeuer, S.; Čáslavský, J.; Pořízka, J.; Ilijević, K. Artificial Cellulose Standards as Calibration Standards for Wavelength-Dispersive X-Ray Fluorescence Analysis of Elements in Plant Samples. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 2021, 502, 106–117. https://doi.org/10.1016/j.nimb.2021.06.012.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4615"
}

Orlić, J., Gržetić, I., Goessler, W., Braeuer, S., Čáslavský, J., Pořízka, J.,& Ilijević, K.. (2021). Supplementary data for the article: Orlić, J.; Gržetić, I.; Goessler, W.; Braeuer, S.; Čáslavský, J.; Pořízka, J.; Ilijević, K. Artificial Cellulose Standards as Calibration Standards for Wavelength-Dispersive X-Ray Fluorescence Analysis of Elements in Plant Samples. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 2021, 502, 106–117. https://doi.org/10.1016/j.nimb.2021.06.012.. in Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_4615

Orlić J, Gržetić I, Goessler W, Braeuer S, Čáslavský J, Pořízka J, Ilijević K. Supplementary data for the article: Orlić, J.; Gržetić, I.; Goessler, W.; Braeuer, S.; Čáslavský, J.; Pořízka, J.; Ilijević, K. Artificial Cellulose Standards as Calibration Standards for Wavelength-Dispersive X-Ray Fluorescence Analysis of Elements in Plant Samples. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 2021, 502, 106–117. https://doi.org/10.1016/j.nimb.2021.06.012.. in Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4615 .

Orlić, Jovana, Gržetić, Ivan, Goessler, Walter, Braeuer, Simone, Čáslavský, Josef, Pořízka, Jaromír, Ilijević, Konstantin, "Supplementary data for the article: Orlić, J.; Gržetić, I.; Goessler, W.; Braeuer, S.; Čáslavský, J.; Pořízka, J.; Ilijević, K. Artificial Cellulose Standards as Calibration Standards for Wavelength-Dispersive X-Ray Fluorescence Analysis of Elements in Plant Samples. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms 2021, 502, 106–117. https://doi.org/10.1016/j.nimb.2021.06.012." in Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4615 .

TY  - JOUR
AU  - Ilijević, Konstantin
AU  - Vujanović, Dragana
AU  - Orčić, Snežana M.
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Zarić, Nenad M.
AU  - Gržetić, Ivan
AU  - Blagojević, Duško P.
AU  - Čelić, Tatjana V.
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4616
AB  - Honeybee colony losses have been a focus of research in the last years, due to the importance of managed honeybee colonies for economy and ecology. Different unfavorable conditions from the outside environment have a strong impact on the hive health. The majority of losses occur mainly during winter and the exact reason is not completely understood. Only a small number of studies are dealing with content of bioelements, their function and influence on honeybee physiology. The aim of the present study was to determine seasonal and spatial variations in content of bioelements and non-essential elements, in hemolymph and whole body of honeybees originating from three regions with different degrees of urbanization and industrialization. Concentrations of 16 elements were compared: macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). Content of bioelements and non-essential elements in honeybees was under noticeable influence of the surrounding environment, season and degree of honeybee activity. Hemolymph was proven to be helpful in differentiating air pollution from other sources of honeybee exposure. The results of our study demonstrated that bees can be successfully used as biomonitors since we have observed statistically significant differences among observed locations, but unless compared locations are exposed to excessively different pollution pressures, it is essential that all bees should be collected at the same season.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
T1  - Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph
VL  - 239
SP  - 108852
DO  - 10.1016/j.cbpc.2020.108852
ER  - 

@article{
author = "Ilijević, Konstantin and Vujanović, Dragana and Orčić, Snežana M. and Purać, Jelena and Kojić, Danijela and Zarić, Nenad M. and Gržetić, Ivan and Blagojević, Duško P. and Čelić, Tatjana V.",
year = "2021",
abstract = "Honeybee colony losses have been a focus of research in the last years, due to the importance of managed honeybee colonies for economy and ecology. Different unfavorable conditions from the outside environment have a strong impact on the hive health. The majority of losses occur mainly during winter and the exact reason is not completely understood. Only a small number of studies are dealing with content of bioelements, their function and influence on honeybee physiology. The aim of the present study was to determine seasonal and spatial variations in content of bioelements and non-essential elements, in hemolymph and whole body of honeybees originating from three regions with different degrees of urbanization and industrialization. Concentrations of 16 elements were compared: macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). Content of bioelements and non-essential elements in honeybees was under noticeable influence of the surrounding environment, season and degree of honeybee activity. Hemolymph was proven to be helpful in differentiating air pollution from other sources of honeybee exposure. The results of our study demonstrated that bees can be successfully used as biomonitors since we have observed statistically significant differences among observed locations, but unless compared locations are exposed to excessively different pollution pressures, it is essential that all bees should be collected at the same season.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology",
title = "Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph",
volume = "239",
pages = "108852",
doi = "10.1016/j.cbpc.2020.108852"
}

Ilijević, K., Vujanović, D., Orčić, S. M., Purać, J., Kojić, D., Zarić, N. M., Gržetić, I., Blagojević, D. P.,& Čelić, T. V.. (2021). Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Elsevier Inc.., 239, 108852.
https://doi.org/10.1016/j.cbpc.2020.108852

Ilijević K, Vujanović D, Orčić SM, Purać J, Kojić D, Zarić NM, Gržetić I, Blagojević DP, Čelić TV. Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology. 2021;239:108852.
doi:10.1016/j.cbpc.2020.108852 .

Ilijević, Konstantin, Vujanović, Dragana, Orčić, Snežana M., Purać, Jelena, Kojić, Danijela, Zarić, Nenad M., Gržetić, Ivan, Blagojević, Duško P., Čelić, Tatjana V., "Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph" in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 239 (2021):108852,
https://doi.org/10.1016/j.cbpc.2020.108852 . .

TY  - DATA
AU  - Ilijević, Konstantin
AU  - Vujanović, Dragana
AU  - Orčić, Snežana M.
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Zarić, Nenad M.
AU  - Gržetić, Ivan
AU  - Blagojević, Duško P.
AU  - Čelić, Tatjana V.
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4617
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
T1  - Supplementary data for the article: Ilijević, K.; Vujanović, D.; Orčić, S.; Purać, J.; Kojić, D.; Zarić, N.; Gržetić, I.; Blagojević, D. P.; Čelić, T. V. Anthropogenic Influence on Seasonal and Spatial Variation in Bioelements and Non-Essential Elements in Honeybees and Their Hemolymph. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 2021, 239, 108852. https://doi.org/10.1016/j.cbpc.2020.108852.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4617
ER  - 

@misc{
author = "Ilijević, Konstantin and Vujanović, Dragana and Orčić, Snežana M. and Purać, Jelena and Kojić, Danijela and Zarić, Nenad M. and Gržetić, Ivan and Blagojević, Duško P. and Čelić, Tatjana V.",
year = "2021",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology",
title = "Supplementary data for the article: Ilijević, K.; Vujanović, D.; Orčić, S.; Purać, J.; Kojić, D.; Zarić, N.; Gržetić, I.; Blagojević, D. P.; Čelić, T. V. Anthropogenic Influence on Seasonal and Spatial Variation in Bioelements and Non-Essential Elements in Honeybees and Their Hemolymph. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 2021, 239, 108852. https://doi.org/10.1016/j.cbpc.2020.108852.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4617"
}

Ilijević, K., Vujanović, D., Orčić, S. M., Purać, J., Kojić, D., Zarić, N. M., Gržetić, I., Blagojević, D. P.,& Čelić, T. V.. (2021). Supplementary data for the article: Ilijević, K.; Vujanović, D.; Orčić, S.; Purać, J.; Kojić, D.; Zarić, N.; Gržetić, I.; Blagojević, D. P.; Čelić, T. V. Anthropogenic Influence on Seasonal and Spatial Variation in Bioelements and Non-Essential Elements in Honeybees and Their Hemolymph. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 2021, 239, 108852. https://doi.org/10.1016/j.cbpc.2020.108852.. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Elsevier Inc...
https://hdl.handle.net/21.15107/rcub_cherry_4617

Ilijević K, Vujanović D, Orčić SM, Purać J, Kojić D, Zarić NM, Gržetić I, Blagojević DP, Čelić TV. Supplementary data for the article: Ilijević, K.; Vujanović, D.; Orčić, S.; Purać, J.; Kojić, D.; Zarić, N.; Gržetić, I.; Blagojević, D. P.; Čelić, T. V. Anthropogenic Influence on Seasonal and Spatial Variation in Bioelements and Non-Essential Elements in Honeybees and Their Hemolymph. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 2021, 239, 108852. https://doi.org/10.1016/j.cbpc.2020.108852.. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4617 .

Ilijević, Konstantin, Vujanović, Dragana, Orčić, Snežana M., Purać, Jelena, Kojić, Danijela, Zarić, Nenad M., Gržetić, Ivan, Blagojević, Duško P., Čelić, Tatjana V., "Supplementary data for the article: Ilijević, K.; Vujanović, D.; Orčić, S.; Purać, J.; Kojić, D.; Zarić, N.; Gržetić, I.; Blagojević, D. P.; Čelić, T. V. Anthropogenic Influence on Seasonal and Spatial Variation in Bioelements and Non-Essential Elements in Honeybees and Their Hemolymph. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 2021, 239, 108852. https://doi.org/10.1016/j.cbpc.2020.108852." in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4617 .

TY  - JOUR
AU  - Orlić, Jovana
AU  - Gržetić, Ivan
AU  - Ilijević, Konstantin
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0584854721002159
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4618
AB  - XRF (X-ray fluorescence analysis) has been applied as the method for analysis of plant samples, but not in its full potential. This method would be highly suitable for monitoring programs and screening studies. A simple sample preparation procedure makes this technique time and cost-effective, although the absence of adequate calibration standards for some analyzed matrices can be a significant limiting factor. Nevertheless, this obstacle can be overcome by the development of the so-called “standardless method” approach. It is important to explore the possibilities and limitations of such method applied to an analysis of plant materials. The accuracy of XRF analysis is highly dependent on the physical characteristics of the sample, which can be significantly affected by the sample preparation procedure. Variations of 3 crucial sample preparation parameters: the mass of the samples (from 1 to 5 g), binder ratio (from 0 to 25% of wax), and pressing pressure (from 10 to 25 t) were tested on needles obtained from two widespread conifer species: Pinus nigra and Abies alba. For most elements, the correlation between the concentration and the change of the binder ratio was statistically significant, while there was no statistically significant correlation between the concentration and the change of the pellet mass and applied pressure. For both types of needles, an estimated systematic error was always higher than a random error. The difference between two types of errors has been higher among samples with different wax content than in samples with different pellet mass, while the variation of applied pressure did not introduce any significant systematic error. On average the change of wax content inflated the value of systematic error by 18 and 13% for the pine and fir needle samples (respectively), while the change of the sample mass has introduced systematic error in lesser extent, on average it was 11% for pine needles and 10% for fir needle samples. The change of pelletizing pressure affected the results by only 3.9% for both conifer needles.
PB  - Elsevier
T2  - Spectrochimica Acta Part B: Atomic Spectroscopy
T1  - Effect of sample preparation procedure on standardless wavelength dispersive X-ray fluorescence analysis of plant samples
VL  - 184
SP  - 106258
DO  - 10.1016/j.sab.2021.106258
ER  - 

@article{
author = "Orlić, Jovana and Gržetić, Ivan and Ilijević, Konstantin",
year = "2021",
abstract = "XRF (X-ray fluorescence analysis) has been applied as the method for analysis of plant samples, but not in its full potential. This method would be highly suitable for monitoring programs and screening studies. A simple sample preparation procedure makes this technique time and cost-effective, although the absence of adequate calibration standards for some analyzed matrices can be a significant limiting factor. Nevertheless, this obstacle can be overcome by the development of the so-called “standardless method” approach. It is important to explore the possibilities and limitations of such method applied to an analysis of plant materials. The accuracy of XRF analysis is highly dependent on the physical characteristics of the sample, which can be significantly affected by the sample preparation procedure. Variations of 3 crucial sample preparation parameters: the mass of the samples (from 1 to 5 g), binder ratio (from 0 to 25% of wax), and pressing pressure (from 10 to 25 t) were tested on needles obtained from two widespread conifer species: Pinus nigra and Abies alba. For most elements, the correlation between the concentration and the change of the binder ratio was statistically significant, while there was no statistically significant correlation between the concentration and the change of the pellet mass and applied pressure. For both types of needles, an estimated systematic error was always higher than a random error. The difference between two types of errors has been higher among samples with different wax content than in samples with different pellet mass, while the variation of applied pressure did not introduce any significant systematic error. On average the change of wax content inflated the value of systematic error by 18 and 13% for the pine and fir needle samples (respectively), while the change of the sample mass has introduced systematic error in lesser extent, on average it was 11% for pine needles and 10% for fir needle samples. The change of pelletizing pressure affected the results by only 3.9% for both conifer needles.",
publisher = "Elsevier",
journal = "Spectrochimica Acta Part B: Atomic Spectroscopy",
title = "Effect of sample preparation procedure on standardless wavelength dispersive X-ray fluorescence analysis of plant samples",
volume = "184",
pages = "106258",
doi = "10.1016/j.sab.2021.106258"
}

Orlić, J., Gržetić, I.,& Ilijević, K.. (2021). Effect of sample preparation procedure on standardless wavelength dispersive X-ray fluorescence analysis of plant samples. in Spectrochimica Acta Part B: Atomic Spectroscopy
Elsevier., 184, 106258.
https://doi.org/10.1016/j.sab.2021.106258

Orlić J, Gržetić I, Ilijević K. Effect of sample preparation procedure on standardless wavelength dispersive X-ray fluorescence analysis of plant samples. in Spectrochimica Acta Part B: Atomic Spectroscopy. 2021;184:106258.
doi:10.1016/j.sab.2021.106258 .

Orlić, Jovana, Gržetić, Ivan, Ilijević, Konstantin, "Effect of sample preparation procedure on standardless wavelength dispersive X-ray fluorescence analysis of plant samples" in Spectrochimica Acta Part B: Atomic Spectroscopy, 184 (2021):106258,
https://doi.org/10.1016/j.sab.2021.106258 . .

TY  - DATA
AU  - Orlić, Jovana
AU  - Gržetić, Ivan
AU  - Ilijević, Konstantin
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0584854721002159
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4619
PB  - Elsevier
T2  - Spectrochimica Acta Part B: Atomic Spectroscopy
T1  - Supplementary data for the article: Orlić, J.; Gržetić, I.; Ilijević, K. Effect of Sample Preparation Procedure on Standardless Wavelength Dispersive X-Ray Fluorescence Analysis of Plant Samples. Spectrochimica Acta Part B: Atomic Spectroscopy 2021, 184, 106258. https://doi.org/10.1016/j.sab.2021.106258.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4619
ER  - 

@misc{
author = "Orlić, Jovana and Gržetić, Ivan and Ilijević, Konstantin",
year = "2021",
publisher = "Elsevier",
journal = "Spectrochimica Acta Part B: Atomic Spectroscopy",
title = "Supplementary data for the article: Orlić, J.; Gržetić, I.; Ilijević, K. Effect of Sample Preparation Procedure on Standardless Wavelength Dispersive X-Ray Fluorescence Analysis of Plant Samples. Spectrochimica Acta Part B: Atomic Spectroscopy 2021, 184, 106258. https://doi.org/10.1016/j.sab.2021.106258.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4619"
}

Orlić, J., Gržetić, I.,& Ilijević, K.. (2021). Supplementary data for the article: Orlić, J.; Gržetić, I.; Ilijević, K. Effect of Sample Preparation Procedure on Standardless Wavelength Dispersive X-Ray Fluorescence Analysis of Plant Samples. Spectrochimica Acta Part B: Atomic Spectroscopy 2021, 184, 106258. https://doi.org/10.1016/j.sab.2021.106258.. in Spectrochimica Acta Part B: Atomic Spectroscopy
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_4619

Orlić J, Gržetić I, Ilijević K. Supplementary data for the article: Orlić, J.; Gržetić, I.; Ilijević, K. Effect of Sample Preparation Procedure on Standardless Wavelength Dispersive X-Ray Fluorescence Analysis of Plant Samples. Spectrochimica Acta Part B: Atomic Spectroscopy 2021, 184, 106258. https://doi.org/10.1016/j.sab.2021.106258.. in Spectrochimica Acta Part B: Atomic Spectroscopy. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4619 .

Orlić, Jovana, Gržetić, Ivan, Ilijević, Konstantin, "Supplementary data for the article: Orlić, J.; Gržetić, I.; Ilijević, K. Effect of Sample Preparation Procedure on Standardless Wavelength Dispersive X-Ray Fluorescence Analysis of Plant Samples. Spectrochimica Acta Part B: Atomic Spectroscopy 2021, 184, 106258. https://doi.org/10.1016/j.sab.2021.106258." in Spectrochimica Acta Part B: Atomic Spectroscopy (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4619 .

TY  - JOUR
AU  - Ilijević, Konstantin
AU  - Vujanović, Dragana
AU  - Orčić, Snežana M.
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Zarić, Nenad M.
AU  - Gržetić, Ivan
AU  - Blagojević, Duško P.
AU  - Čelić, Tatjana V.
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5759
AB  - Honeybee colony losses have been a focus of research in the last years, due to the importance of managed honeybee colonies for economy and ecology. Different unfavorable conditions from the outside environment have a strong impact on the hive health. The majority of losses occur mainly during winter and the exact reason is not completely understood. Only a small number of studies are dealing with content of bioelements, their function and influence on honeybee physiology. The aim of the present study was to determine seasonal and spatial variations in content of bioelements and non-essential elements, in hemolymph and whole body of honeybees originating from three regions with different degrees of urbanization and industrialization. Concentrations of 16 elements were compared: macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). Content of bioelements and non-essential elements in honeybees was under noticeable influence of the surrounding environment, season and degree of honeybee activity. Hemolymph was proven to be helpful in differentiating air pollution from other sources of honeybee exposure. The results of our study demonstrated that bees can be successfully used as biomonitors since we have observed statistically significant differences among observed locations, but unless compared locations are exposed to excessively different pollution pressures, it is essential that all bees should be collected at the same season.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
T1  - Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph
VL  - 239
SP  - 108852
DO  - 10.1016/j.cbpc.2020.108852
ER  - 

@article{
author = "Ilijević, Konstantin and Vujanović, Dragana and Orčić, Snežana M. and Purać, Jelena and Kojić, Danijela and Zarić, Nenad M. and Gržetić, Ivan and Blagojević, Duško P. and Čelić, Tatjana V.",
year = "2021",
abstract = "Honeybee colony losses have been a focus of research in the last years, due to the importance of managed honeybee colonies for economy and ecology. Different unfavorable conditions from the outside environment have a strong impact on the hive health. The majority of losses occur mainly during winter and the exact reason is not completely understood. Only a small number of studies are dealing with content of bioelements, their function and influence on honeybee physiology. The aim of the present study was to determine seasonal and spatial variations in content of bioelements and non-essential elements, in hemolymph and whole body of honeybees originating from three regions with different degrees of urbanization and industrialization. Concentrations of 16 elements were compared: macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). Content of bioelements and non-essential elements in honeybees was under noticeable influence of the surrounding environment, season and degree of honeybee activity. Hemolymph was proven to be helpful in differentiating air pollution from other sources of honeybee exposure. The results of our study demonstrated that bees can be successfully used as biomonitors since we have observed statistically significant differences among observed locations, but unless compared locations are exposed to excessively different pollution pressures, it is essential that all bees should be collected at the same season.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology",
title = "Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph",
volume = "239",
pages = "108852",
doi = "10.1016/j.cbpc.2020.108852"
}

Ilijević, K., Vujanović, D., Orčić, S. M., Purać, J., Kojić, D., Zarić, N. M., Gržetić, I., Blagojević, D. P.,& Čelić, T. V.. (2021). Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Elsevier Inc.., 239, 108852.
https://doi.org/10.1016/j.cbpc.2020.108852

Ilijević K, Vujanović D, Orčić SM, Purać J, Kojić D, Zarić NM, Gržetić I, Blagojević DP, Čelić TV. Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology. 2021;239:108852.
doi:10.1016/j.cbpc.2020.108852 .

Ilijević, Konstantin, Vujanović, Dragana, Orčić, Snežana M., Purać, Jelena, Kojić, Danijela, Zarić, Nenad M., Gržetić, Ivan, Blagojević, Duško P., Čelić, Tatjana V., "Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph" in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 239 (2021):108852,
https://doi.org/10.1016/j.cbpc.2020.108852 . .

TY  - JOUR
AU  - Orlić, Jovana
AU  - Gržetić, Ivan
AU  - Goessler, Walter
AU  - Braeuer, Simone
AU  - Čáslavský, Josef
AU  - Pořízka, Jaromír
AU  - Ilijević, Konstantin
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0168583X21002238
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5760
AB  - This research explores the possibilities and limitations of WD-XRF, applied as a method for quantification of 20 elements in plant material, using spiked cellulose standards for calibration. Three different analytical methods were investigated: 1) standards created from pure spiked cellulose; 2) spiked cellulose mixed with 20% of binder and 3) spiked cellulose applied as a thin layer on an inert carrier. Sensitivity, linearity, limit of detection, limit of quantification, repeatability, intralaboratory reproducibility, and accuracy were determined and compared. The accuracy of the investigated methods was tested by analysis of standard reference materials and comparison with other routinely used analytical techniques (ICP-OES and ICP-MS). The comparison included real plant samples which were collected from the environment characterized by different pollution levels. The accuracy of the semiquantitative standardless method was also considered and compared with other investigated methods. Tested methods can be very precise, with good intralaboratory reproducibility over wide linear range.
PB  - Elsevier
T2  - Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
T1  - Artificial cellulose standards as calibration standards for wavelength-dispersive X-ray fluorescence analysis of elements in plant samples
VL  - 502
SP  - 106
EP  - 117
DO  - 10.1016/j.nimb.2021.06.012
ER  - 

@article{
author = "Orlić, Jovana and Gržetić, Ivan and Goessler, Walter and Braeuer, Simone and Čáslavský, Josef and Pořízka, Jaromír and Ilijević, Konstantin",
year = "2021",
abstract = "This research explores the possibilities and limitations of WD-XRF, applied as a method for quantification of 20 elements in plant material, using spiked cellulose standards for calibration. Three different analytical methods were investigated: 1) standards created from pure spiked cellulose; 2) spiked cellulose mixed with 20% of binder and 3) spiked cellulose applied as a thin layer on an inert carrier. Sensitivity, linearity, limit of detection, limit of quantification, repeatability, intralaboratory reproducibility, and accuracy were determined and compared. The accuracy of the investigated methods was tested by analysis of standard reference materials and comparison with other routinely used analytical techniques (ICP-OES and ICP-MS). The comparison included real plant samples which were collected from the environment characterized by different pollution levels. The accuracy of the semiquantitative standardless method was also considered and compared with other investigated methods. Tested methods can be very precise, with good intralaboratory reproducibility over wide linear range.",
publisher = "Elsevier",
journal = "Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms",
title = "Artificial cellulose standards as calibration standards for wavelength-dispersive X-ray fluorescence analysis of elements in plant samples",
volume = "502",
pages = "106-117",
doi = "10.1016/j.nimb.2021.06.012"
}

Orlić, J., Gržetić, I., Goessler, W., Braeuer, S., Čáslavský, J., Pořízka, J.,& Ilijević, K.. (2021). Artificial cellulose standards as calibration standards for wavelength-dispersive X-ray fluorescence analysis of elements in plant samples. in Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
Elsevier., 502, 106-117.
https://doi.org/10.1016/j.nimb.2021.06.012

Orlić J, Gržetić I, Goessler W, Braeuer S, Čáslavský J, Pořízka J, Ilijević K. Artificial cellulose standards as calibration standards for wavelength-dispersive X-ray fluorescence analysis of elements in plant samples. in Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. 2021;502:106-117.
doi:10.1016/j.nimb.2021.06.012 .

Orlić, Jovana, Gržetić, Ivan, Goessler, Walter, Braeuer, Simone, Čáslavský, Josef, Pořízka, Jaromír, Ilijević, Konstantin, "Artificial cellulose standards as calibration standards for wavelength-dispersive X-ray fluorescence analysis of elements in plant samples" in Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 502 (2021):106-117,
https://doi.org/10.1016/j.nimb.2021.06.012 . .

TY  - JOUR
AU  - Orlić, Jovana
AU  - Gržetić, Ivan
AU  - Ilijević, Konstantin
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0584854721002159
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5761
AB  - XRF (X-ray fluorescence analysis) has been applied as the method for analysis of plant samples, but not in its full potential. This method would be highly suitable for monitoring programs and screening studies. A simple sample preparation procedure makes this technique time and cost-effective, although the absence of adequate calibration standards for some analyzed matrices can be a significant limiting factor. Nevertheless, this obstacle can be overcome by the development of the so-called “standardless method” approach. It is important to explore the possibilities and limitations of such method applied to an analysis of plant materials. The accuracy of XRF analysis is highly dependent on the physical characteristics of the sample, which can be significantly affected by the sample preparation procedure. Variations of 3 crucial sample preparation parameters: the mass of the samples (from 1 to 5 g), binder ratio (from 0 to 25% of wax), and pressing pressure (from 10 to 25 t) were tested on needles obtained from two widespread conifer species: Pinus nigra and Abies alba. For most elements, the correlation between the concentration and the change of the binder ratio was statistically significant, while there was no statistically significant correlation between the concentration and the change of the pellet mass and applied pressure. For both types of needles, an estimated systematic error was always higher than a random error. The difference between two types of errors has been higher among samples with different wax content than in samples with different pellet mass, while the variation of applied pressure did not introduce any significant systematic error. On average the change of wax content inflated the value of systematic error by 18 and 13% for the pine and fir needle samples (respectively), while the change of the sample mass has introduced systematic error in lesser extent, on average it was 11% for pine needles and 10% for fir needle samples. The change of pelletizing pressure affected the results by only 3.9% for both conifer needles.
PB  - Elsevier
T2  - Spectrochimica Acta Part B: Atomic Spectroscopy
T1  - Effect of sample preparation procedure on standardless wavelength dispersive X-ray fluorescence analysis of plant samples
VL  - 184
SP  - 106258
DO  - 10.1016/j.sab.2021.106258
ER  - 

@article{
author = "Orlić, Jovana and Gržetić, Ivan and Ilijević, Konstantin",
year = "2021",
abstract = "XRF (X-ray fluorescence analysis) has been applied as the method for analysis of plant samples, but not in its full potential. This method would be highly suitable for monitoring programs and screening studies. A simple sample preparation procedure makes this technique time and cost-effective, although the absence of adequate calibration standards for some analyzed matrices can be a significant limiting factor. Nevertheless, this obstacle can be overcome by the development of the so-called “standardless method” approach. It is important to explore the possibilities and limitations of such method applied to an analysis of plant materials. The accuracy of XRF analysis is highly dependent on the physical characteristics of the sample, which can be significantly affected by the sample preparation procedure. Variations of 3 crucial sample preparation parameters: the mass of the samples (from 1 to 5 g), binder ratio (from 0 to 25% of wax), and pressing pressure (from 10 to 25 t) were tested on needles obtained from two widespread conifer species: Pinus nigra and Abies alba. For most elements, the correlation between the concentration and the change of the binder ratio was statistically significant, while there was no statistically significant correlation between the concentration and the change of the pellet mass and applied pressure. For both types of needles, an estimated systematic error was always higher than a random error. The difference between two types of errors has been higher among samples with different wax content than in samples with different pellet mass, while the variation of applied pressure did not introduce any significant systematic error. On average the change of wax content inflated the value of systematic error by 18 and 13% for the pine and fir needle samples (respectively), while the change of the sample mass has introduced systematic error in lesser extent, on average it was 11% for pine needles and 10% for fir needle samples. The change of pelletizing pressure affected the results by only 3.9% for both conifer needles.",
publisher = "Elsevier",
journal = "Spectrochimica Acta Part B: Atomic Spectroscopy",
title = "Effect of sample preparation procedure on standardless wavelength dispersive X-ray fluorescence analysis of plant samples",
volume = "184",
pages = "106258",
doi = "10.1016/j.sab.2021.106258"
}

Orlić, J., Gržetić, I.,& Ilijević, K.. (2021). Effect of sample preparation procedure on standardless wavelength dispersive X-ray fluorescence analysis of plant samples. in Spectrochimica Acta Part B: Atomic Spectroscopy
Elsevier., 184, 106258.
https://doi.org/10.1016/j.sab.2021.106258

Orlić J, Gržetić I, Ilijević K. Effect of sample preparation procedure on standardless wavelength dispersive X-ray fluorescence analysis of plant samples. in Spectrochimica Acta Part B: Atomic Spectroscopy. 2021;184:106258.
doi:10.1016/j.sab.2021.106258 .

Orlić, Jovana, Gržetić, Ivan, Ilijević, Konstantin, "Effect of sample preparation procedure on standardless wavelength dispersive X-ray fluorescence analysis of plant samples" in Spectrochimica Acta Part B: Atomic Spectroscopy, 184 (2021):106258,
https://doi.org/10.1016/j.sab.2021.106258 . .

TY  - CONF
AU  - Ilijević, Konstantin
AU  - Vujanović, D.
AU  - Zarić, N. M.
AU  - Orlić, Jovana
AU  - Orčić, S.
AU  - Purać, J.
AU  - Kojić, D.
AU  - Blagojević, D. P.
AU  - Čelić, T.
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4911
AB  - In our previous research we have explored concentrations of 16 elements in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). These three locations were chosen due to their distinctly different degrees of urbanization and industrialization. Macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) were determined in the whole body of honeybees, but the major novelty of the research was that hemolymph of the bees was analysed as well. Significant spatial but also seasonal variations in content of bioelements and non-essential elements were observed. These findings have raised several important questions which are addressed in our current study. In order to better understand how bees’ environment does affects concentrations of elements mentioned above, dust and pollen collected from the same locations were analysed. They represent 2 major sources of bio elements and toxic elements for the bees: food and atmospheric deposition. For the better understanding of dynamics of investigated elements the scope of our research was further extended to the analysis of bee bread, honey, crops, wax, propolis, larvae and pupae. The samples were digested in accordance with the US EPA SW-846 Method 3052. Closed microwave digestion system (ETHOS 1, Advanced Microwave Digestion System, Milestone, Italy) was used for digestion with 5 to 8 ml of concentrated HNO3  and 1 or 2 ml of concentrated H2 O2  (depending on the mass and type of the sample). Concentrations of: Al, Ba, Cd, Co, Cr, Cu, Ca, Fe, K, Mg, Mn, Na, Ni, Pb, Sr and Zn were determined by ICP-OES (iCAP 6500Duo, Thermo Scientific). Very low concentrations of: Co, Cr, Cd and Pb, which occurred in some samples were confirmed by ICP-MS (iCAP-Q-ICP-MS, Termo Scientific). Ratios between concentrations in the samples from industrial region and urban region were calculated and compared for different matrices. Concentrations of toxic metals such as Pb and Cd were significantly elevated in dust samples from the industrial site, and similar trend was observed for pollen, bee bread, wax, propolis, and the whole bees. Elevation of concentrations was not observed (or it was present in significantly lesser extent) for the samples of honey, larvae and pupae.
PB  - Belgrade : Serbian Chemical Society
C3  - Book of Abstracts 21st; European Meeting on Environmental Chemistry
T1  - Bioelements and Non-Essential Elements in Honeybees and Their Hemolymph, Larvae, Pupae, Honey, Wax, Propolis and Bee Bread
SP  - 81
EP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4911
ER  - 

@conference{
author = "Ilijević, Konstantin and Vujanović, D. and Zarić, N. M. and Orlić, Jovana and Orčić, S. and Purać, J. and Kojić, D. and Blagojević, D. P. and Čelić, T.",
year = "2021",
abstract = "In our previous research we have explored concentrations of 16 elements in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). These three locations were chosen due to their distinctly different degrees of urbanization and industrialization. Macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) were determined in the whole body of honeybees, but the major novelty of the research was that hemolymph of the bees was analysed as well. Significant spatial but also seasonal variations in content of bioelements and non-essential elements were observed. These findings have raised several important questions which are addressed in our current study. In order to better understand how bees’ environment does affects concentrations of elements mentioned above, dust and pollen collected from the same locations were analysed. They represent 2 major sources of bio elements and toxic elements for the bees: food and atmospheric deposition. For the better understanding of dynamics of investigated elements the scope of our research was further extended to the analysis of bee bread, honey, crops, wax, propolis, larvae and pupae. The samples were digested in accordance with the US EPA SW-846 Method 3052. Closed microwave digestion system (ETHOS 1, Advanced Microwave Digestion System, Milestone, Italy) was used for digestion with 5 to 8 ml of concentrated HNO3  and 1 or 2 ml of concentrated H2 O2  (depending on the mass and type of the sample). Concentrations of: Al, Ba, Cd, Co, Cr, Cu, Ca, Fe, K, Mg, Mn, Na, Ni, Pb, Sr and Zn were determined by ICP-OES (iCAP 6500Duo, Thermo Scientific). Very low concentrations of: Co, Cr, Cd and Pb, which occurred in some samples were confirmed by ICP-MS (iCAP-Q-ICP-MS, Termo Scientific). Ratios between concentrations in the samples from industrial region and urban region were calculated and compared for different matrices. Concentrations of toxic metals such as Pb and Cd were significantly elevated in dust samples from the industrial site, and similar trend was observed for pollen, bee bread, wax, propolis, and the whole bees. Elevation of concentrations was not observed (or it was present in significantly lesser extent) for the samples of honey, larvae and pupae.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Book of Abstracts 21st; European Meeting on Environmental Chemistry",
title = "Bioelements and Non-Essential Elements in Honeybees and Their Hemolymph, Larvae, Pupae, Honey, Wax, Propolis and Bee Bread",
pages = "81-81",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4911"
}

Ilijević, K., Vujanović, D., Zarić, N. M., Orlić, J., Orčić, S., Purać, J., Kojić, D., Blagojević, D. P.,& Čelić, T.. (2021). Bioelements and Non-Essential Elements in Honeybees and Their Hemolymph, Larvae, Pupae, Honey, Wax, Propolis and Bee Bread. in Book of Abstracts 21st; European Meeting on Environmental Chemistry
Belgrade : Serbian Chemical Society., 81-81.
https://hdl.handle.net/21.15107/rcub_cherry_4911

Ilijević K, Vujanović D, Zarić NM, Orlić J, Orčić S, Purać J, Kojić D, Blagojević DP, Čelić T. Bioelements and Non-Essential Elements in Honeybees and Their Hemolymph, Larvae, Pupae, Honey, Wax, Propolis and Bee Bread. in Book of Abstracts 21st; European Meeting on Environmental Chemistry. 2021;:81-81.
https://hdl.handle.net/21.15107/rcub_cherry_4911 .

Ilijević, Konstantin, Vujanović, D., Zarić, N. M., Orlić, Jovana, Orčić, S., Purać, J., Kojić, D., Blagojević, D. P., Čelić, T., "Bioelements and Non-Essential Elements in Honeybees and Their Hemolymph, Larvae, Pupae, Honey, Wax, Propolis and Bee Bread" in Book of Abstracts 21st; European Meeting on Environmental Chemistry (2021):81-81,
https://hdl.handle.net/21.15107/rcub_cherry_4911 .

TY  - CONF
AU  - Orlić, Jovana
AU  - Kukobat, L.
AU  - Vidojević, D.
AU  - Filipović, S.
AU  - Kojić, D.
AU  - Blagojević, D. P.
AU  - Ilijević, Konstantin
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4913
AB  - Toxic metals in soil are routinely determined by several analytical spectroscopic techniques (Atomic Absorption Spectrometry AAS, Inductively Coupled Plasma Optical Emission Spectrometry ICP-OES,and Inductively Coupled Plasma Mass Spectrometry ICPMS)[1]. Those techniques measure metals from aqueous samples. Procedures of sample dissolution or extraction typically involve a lengthy process which requires the use of harsh conditions. Sample preparation procedures make these routinely used techniques generally time-consuming and too expensive [2]. On the other side, the need for reliable, economical, and environmental friendly technique for soil composition measuring has been growing in the environmental field, so has the demand for time and cost-efficient analytical methods for soil analysis [3]. X-ray fluorescence spectrometry (XRF) is a multi-element analytical technique for direct, non-destructive analysis of various materials (including soils) with minimal sample preparation. The most attractive advantage of XRF is the wide dynamic range (from mg kg-1 to 100%). A portable X-ray fluorescence spectrometer (PXRF) is also capable of in-situ analysis in a short time (30–120 s) [4]. In situ PXRF analysis provides flexibility and allows rapid collection of data for a large number of samples, andproduces real-time data that can be used for rapid decision making. It is well-known that the physical characteristics of the sample play an important role in obtaining accurate results when it comes to XRF methods. Therefore it is important to determine how reliable in situ PXRF results are. Analytical accuracy and precision could be generally improved if adequate sample preparation procedure is applied compared to in situ measurements. The aim of this research was to determinate in what extent sample preparation procedure changes measured concentrations of elements and is that change the same for all investigated elements. Does soil sample homogenization or further pressing into the compact pellet systematically affect measured concentrations? Soil samples from 32 industrial, potentially contaminated sites were collected from a depth of 10 cm, 30 cm, and 50 cm. Such soils provide wide concentration range of different elements. Samples were first directly analyzed in the field, without any sample preparation using the Thermo Scientific™ Niton™ XL3t GOLDD+ PXRF Analyzer. The second PXRF analysis was performed in the laboratory on the dry,ground, and homogenized soil powder sample. One aliquot of soil powder was digested for AAS analysis, while another aliquot was pressed into a 32 mm diameter pellet and analyzed using PXRF. The quality control program involves comparison of the results with AAS reference technique. Additionally, certified reference materials of stream sediment (STSD-3) and soil (NCS DC 77301) are analyzed with different sample preparation procedures.
PB  - Belgrade : Serbian Chemical Society
C3  - Book of Abstracts 21st; European Meeting on Environmental Chemistry
T1  - Effect of Sample Preparation on Portable X-Ray Fluorescence Spectrometry Analysis of Contaminated Soils
SP  - 93
EP  - 93
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4913
ER  - 

@conference{
author = "Orlić, Jovana and Kukobat, L. and Vidojević, D. and Filipović, S. and Kojić, D. and Blagojević, D. P. and Ilijević, Konstantin",
year = "2021",
abstract = "Toxic metals in soil are routinely determined by several analytical spectroscopic techniques (Atomic Absorption Spectrometry AAS, Inductively Coupled Plasma Optical Emission Spectrometry ICP-OES,and Inductively Coupled Plasma Mass Spectrometry ICPMS)[1]. Those techniques measure metals from aqueous samples. Procedures of sample dissolution or extraction typically involve a lengthy process which requires the use of harsh conditions. Sample preparation procedures make these routinely used techniques generally time-consuming and too expensive [2]. On the other side, the need for reliable, economical, and environmental friendly technique for soil composition measuring has been growing in the environmental field, so has the demand for time and cost-efficient analytical methods for soil analysis [3]. X-ray fluorescence spectrometry (XRF) is a multi-element analytical technique for direct, non-destructive analysis of various materials (including soils) with minimal sample preparation. The most attractive advantage of XRF is the wide dynamic range (from mg kg-1 to 100%). A portable X-ray fluorescence spectrometer (PXRF) is also capable of in-situ analysis in a short time (30–120 s) [4]. In situ PXRF analysis provides flexibility and allows rapid collection of data for a large number of samples, andproduces real-time data that can be used for rapid decision making. It is well-known that the physical characteristics of the sample play an important role in obtaining accurate results when it comes to XRF methods. Therefore it is important to determine how reliable in situ PXRF results are. Analytical accuracy and precision could be generally improved if adequate sample preparation procedure is applied compared to in situ measurements. The aim of this research was to determinate in what extent sample preparation procedure changes measured concentrations of elements and is that change the same for all investigated elements. Does soil sample homogenization or further pressing into the compact pellet systematically affect measured concentrations? Soil samples from 32 industrial, potentially contaminated sites were collected from a depth of 10 cm, 30 cm, and 50 cm. Such soils provide wide concentration range of different elements. Samples were first directly analyzed in the field, without any sample preparation using the Thermo Scientific™ Niton™ XL3t GOLDD+ PXRF Analyzer. The second PXRF analysis was performed in the laboratory on the dry,ground, and homogenized soil powder sample. One aliquot of soil powder was digested for AAS analysis, while another aliquot was pressed into a 32 mm diameter pellet and analyzed using PXRF. The quality control program involves comparison of the results with AAS reference technique. Additionally, certified reference materials of stream sediment (STSD-3) and soil (NCS DC 77301) are analyzed with different sample preparation procedures.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Book of Abstracts 21st; European Meeting on Environmental Chemistry",
title = "Effect of Sample Preparation on Portable X-Ray Fluorescence Spectrometry Analysis of Contaminated Soils",
pages = "93-93",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4913"
}

Orlić, J., Kukobat, L., Vidojević, D., Filipović, S., Kojić, D., Blagojević, D. P.,& Ilijević, K.. (2021). Effect of Sample Preparation on Portable X-Ray Fluorescence Spectrometry Analysis of Contaminated Soils. in Book of Abstracts 21st; European Meeting on Environmental Chemistry
Belgrade : Serbian Chemical Society., 93-93.
https://hdl.handle.net/21.15107/rcub_cherry_4913

Orlić J, Kukobat L, Vidojević D, Filipović S, Kojić D, Blagojević DP, Ilijević K. Effect of Sample Preparation on Portable X-Ray Fluorescence Spectrometry Analysis of Contaminated Soils. in Book of Abstracts 21st; European Meeting on Environmental Chemistry. 2021;:93-93.
https://hdl.handle.net/21.15107/rcub_cherry_4913 .

Orlić, Jovana, Kukobat, L., Vidojević, D., Filipović, S., Kojić, D., Blagojević, D. P., Ilijević, Konstantin, "Effect of Sample Preparation on Portable X-Ray Fluorescence Spectrometry Analysis of Contaminated Soils" in Book of Abstracts 21st; European Meeting on Environmental Chemistry (2021):93-93,
https://hdl.handle.net/21.15107/rcub_cherry_4913 .

TY  - CONF
AU  - Ilijević, Konstantin
AU  - Orlić, Jovana
AU  - Milisavljević, N.
AU  - Zelenović, J.
AU  - Kukobat, L.
AU  - Vidojević, D.
AU  - Zarić, N. M.
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4923
AB  - Kostolac is a town exposed to several serious sources of toxic metals and other inorganic pollutants. They arrive from sources typical for urban environments such as traffic, but also from various heavy industry sources: coal mining, burning of coal in power plants, ash landfills, and steel factory. Toxic metals in the air are concentrated in particulate matter. Their transport and health risks depend strongly on the size of dust particles. Goals of the research were to estimate: 1. how much does traffic contributes to the total pollution load compared to the natural sources and the industry; 2. how is pollution distributed in different fractions of the dust; 3. are there any spatial trends present and is there any correlation between vicinity of pollution sources and concentrations of toxic elements in different fractions of the dust. Samples of dust were collected from 10 locations in July and in September. Each location had one sampling site on a major road with intensive traffic and the other site on auxiliary road with much less traffic, located 10- 20 m away from the major road. The dust was dried, sieved through sieves with 3 different apertures (d=63µm, 250µm and 1000µm) and pressed into 32 mm diameter pellets. The samples were analysed by WD-XRF standardless method. The results showed that Al, P, K, V, Mn, Fe, Co, Zr, Rb and Ti have the highest concentrations in the smallest fraction (d<63µm) and the lowest concentrations in the most coarse fraction with stat. significant differences among concentrations. Concentrations of: Mg, S, Zn and Cu have the same trend as previous group of elements but no stat. significant differences, wile conc. of Si and Ca have the opposite trend. Neither the time of the year nor the intensity of the traffic have had any significant effect to the concentrations, therefore it can be concluded that industrial sources of pollution have significantly higher attribution to the total pollution load than traffic. The trend that toxic elements are more concentrated in the smallest fraction of the dust indicates that the source of the pollution is rather anthropogenic than natural. Concentrations of elements in dust collected on sites from our research were compared to concentrations of the same elements in the soil collected by SEPA (Serbian Environmental Protection Agency). Although locations from both researches were in close proximity, no significant correlation between concentrations was observed. The lack of correlation can be explained by several hypotheses which should be further investigated in future researches.
PB  - Belgrade : Serbian Chemical Society
C3  - Book of Abstracts 21st; European Meeting on Environmental Chemistry
T1  - Toxic Metals in 3 Fractions (d<63µm, d63-250µm and d250-1000µm) of Dust Collected on Roads of Industrial Town Kostolac, Serbia
SP  - 139
EP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4923
ER  - 

@conference{
author = "Ilijević, Konstantin and Orlić, Jovana and Milisavljević, N. and Zelenović, J. and Kukobat, L. and Vidojević, D. and Zarić, N. M.",
year = "2021",
abstract = "Kostolac is a town exposed to several serious sources of toxic metals and other inorganic pollutants. They arrive from sources typical for urban environments such as traffic, but also from various heavy industry sources: coal mining, burning of coal in power plants, ash landfills, and steel factory. Toxic metals in the air are concentrated in particulate matter. Their transport and health risks depend strongly on the size of dust particles. Goals of the research were to estimate: 1. how much does traffic contributes to the total pollution load compared to the natural sources and the industry; 2. how is pollution distributed in different fractions of the dust; 3. are there any spatial trends present and is there any correlation between vicinity of pollution sources and concentrations of toxic elements in different fractions of the dust. Samples of dust were collected from 10 locations in July and in September. Each location had one sampling site on a major road with intensive traffic and the other site on auxiliary road with much less traffic, located 10- 20 m away from the major road. The dust was dried, sieved through sieves with 3 different apertures (d=63µm, 250µm and 1000µm) and pressed into 32 mm diameter pellets. The samples were analysed by WD-XRF standardless method. The results showed that Al, P, K, V, Mn, Fe, Co, Zr, Rb and Ti have the highest concentrations in the smallest fraction (d<63µm) and the lowest concentrations in the most coarse fraction with stat. significant differences among concentrations. Concentrations of: Mg, S, Zn and Cu have the same trend as previous group of elements but no stat. significant differences, wile conc. of Si and Ca have the opposite trend. Neither the time of the year nor the intensity of the traffic have had any significant effect to the concentrations, therefore it can be concluded that industrial sources of pollution have significantly higher attribution to the total pollution load than traffic. The trend that toxic elements are more concentrated in the smallest fraction of the dust indicates that the source of the pollution is rather anthropogenic than natural. Concentrations of elements in dust collected on sites from our research were compared to concentrations of the same elements in the soil collected by SEPA (Serbian Environmental Protection Agency). Although locations from both researches were in close proximity, no significant correlation between concentrations was observed. The lack of correlation can be explained by several hypotheses which should be further investigated in future researches.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Book of Abstracts 21st; European Meeting on Environmental Chemistry",
title = "Toxic Metals in 3 Fractions (d<63µm, d63-250µm and d250-1000µm) of Dust Collected on Roads of Industrial Town Kostolac, Serbia",
pages = "139-139",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4923"
}

Ilijević, K., Orlić, J., Milisavljević, N., Zelenović, J., Kukobat, L., Vidojević, D.,& Zarić, N. M.. (2021). Toxic Metals in 3 Fractions (d<63µm, d63-250µm and d250-1000µm) of Dust Collected on Roads of Industrial Town Kostolac, Serbia. in Book of Abstracts 21st; European Meeting on Environmental Chemistry
Belgrade : Serbian Chemical Society., 139-139.
https://hdl.handle.net/21.15107/rcub_cherry_4923

Ilijević K, Orlić J, Milisavljević N, Zelenović J, Kukobat L, Vidojević D, Zarić NM. Toxic Metals in 3 Fractions (d<63µm, d63-250µm and d250-1000µm) of Dust Collected on Roads of Industrial Town Kostolac, Serbia. in Book of Abstracts 21st; European Meeting on Environmental Chemistry. 2021;:139-139.
https://hdl.handle.net/21.15107/rcub_cherry_4923 .

Ilijević, Konstantin, Orlić, Jovana, Milisavljević, N., Zelenović, J., Kukobat, L., Vidojević, D., Zarić, N. M., "Toxic Metals in 3 Fractions (d<63µm, d63-250µm and d250-1000µm) of Dust Collected on Roads of Industrial Town Kostolac, Serbia" in Book of Abstracts 21st; European Meeting on Environmental Chemistry (2021):139-139,
https://hdl.handle.net/21.15107/rcub_cherry_4923 .

TY  - JOUR
AU  - Životić, Dragana R.
AU  - Cvetković, Olga
AU  - Vulić, Predrag J.
AU  - Gržetić, Ivan
AU  - Simić, Vladimir
AU  - Ilijević, Konstantin
AU  - Dojčinović, Biljana P.
AU  - Erić, Suzana
AU  - Radić, Bogdan
AU  - Stojadinović, Sanja M.
AU  - Trifunović, Snežana S.
PY  - 2019
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2897
AB  - A geochemical and mineralogical study was performed on lignite samples from the Upper Miocene Kovin deposit, hosting three coal seams. The Kovin lignite is characterized by high moisture content, medium to high ash yield, medium to high sulphur content and a relatively low gross and net calorific value. The mineralogical composition, and major and trace element contents were determined by X-ray diffraction, scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDS) analyses, and inductively coupled plasma optical emission spectrometry (ICP-OES). The most abundant minerals in all lignite samples from the three coal seams are clays (illite/smectite), silicates (quartz, plagioclase), sulphates (gypsum/anhydrite) and carbonate (calcite). The other iron-rich minerals are sulphides, oxides and hydroxides (pyrite, mag-netite, haematite, and limonite). In general, mineral matter in the matrix coal consists of illite/ smectite and quartz, while xylite-rich coals, apart from illite/smectite, have a higher content of sulphates and Fe-oxide/hydroxide minerals. The lignite from the Kovin deposit is enriched in As, Cd, Co, Cr, Cu, Ga, Li, Mn, Mo, Ni, Pb, V, Zn, Gd, Tb, Er and Lu in comparison with the Clarke values for brown coals. The statistical analysis of bulk compositional data shows inorganic affinity for the majority of the major and trace elements and possible association with pyrite, illite/ smectite and calcite.
PB  - Crossref
T2  - Geologia Croatica
T1  - Distribution of major and trace elements in the Kovin lignite (Serbia)
VL  - 72
IS  - 1
SP  - 51
EP  - 79
DO  - 10.4154/gc.2019.06
ER  - 

@article{
author = "Životić, Dragana R. and Cvetković, Olga and Vulić, Predrag J. and Gržetić, Ivan and Simić, Vladimir and Ilijević, Konstantin and Dojčinović, Biljana P. and Erić, Suzana and Radić, Bogdan and Stojadinović, Sanja M. and Trifunović, Snežana S.",
year = "2019, 2019",
abstract = "A geochemical and mineralogical study was performed on lignite samples from the Upper Miocene Kovin deposit, hosting three coal seams. The Kovin lignite is characterized by high moisture content, medium to high ash yield, medium to high sulphur content and a relatively low gross and net calorific value. The mineralogical composition, and major and trace element contents were determined by X-ray diffraction, scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDS) analyses, and inductively coupled plasma optical emission spectrometry (ICP-OES). The most abundant minerals in all lignite samples from the three coal seams are clays (illite/smectite), silicates (quartz, plagioclase), sulphates (gypsum/anhydrite) and carbonate (calcite). The other iron-rich minerals are sulphides, oxides and hydroxides (pyrite, mag-netite, haematite, and limonite). In general, mineral matter in the matrix coal consists of illite/ smectite and quartz, while xylite-rich coals, apart from illite/smectite, have a higher content of sulphates and Fe-oxide/hydroxide minerals. The lignite from the Kovin deposit is enriched in As, Cd, Co, Cr, Cu, Ga, Li, Mn, Mo, Ni, Pb, V, Zn, Gd, Tb, Er and Lu in comparison with the Clarke values for brown coals. The statistical analysis of bulk compositional data shows inorganic affinity for the majority of the major and trace elements and possible association with pyrite, illite/ smectite and calcite.",
publisher = "Crossref",
journal = "Geologia Croatica",
title = "Distribution of major and trace elements in the Kovin lignite (Serbia)",
volume = "72",
number = "1",
pages = "51-79",
doi = "10.4154/gc.2019.06"
}

Životić, D. R., Cvetković, O., Vulić, P. J., Gržetić, I., Simić, V., Ilijević, K., Dojčinović, B. P., Erić, S., Radić, B., Stojadinović, S. M.,& Trifunović, S. S.. (2019). Distribution of major and trace elements in the Kovin lignite (Serbia). in Geologia Croatica
Crossref., 72(1), 51-79.
https://doi.org/10.4154/gc.2019.06

Životić DR, Cvetković O, Vulić PJ, Gržetić I, Simić V, Ilijević K, Dojčinović BP, Erić S, Radić B, Stojadinović SM, Trifunović SS. Distribution of major and trace elements in the Kovin lignite (Serbia). in Geologia Croatica. 2019;72(1):51-79.
doi:10.4154/gc.2019.06 .

Životić, Dragana R., Cvetković, Olga, Vulić, Predrag J., Gržetić, Ivan, Simić, Vladimir, Ilijević, Konstantin, Dojčinović, Biljana P., Erić, Suzana, Radić, Bogdan, Stojadinović, Sanja M., Trifunović, Snežana S., "Distribution of major and trace elements in the Kovin lignite (Serbia)" in Geologia Croatica, 72, no. 1 (2019):51-79,
https://doi.org/10.4154/gc.2019.06 . .

TY  - JOUR
AU  - Kojić, Danijela
AU  - Purać, Jelena
AU  - Nikolić, Tatjana V.
AU  - Orčić, Snežana M.
AU  - Vujanović, Dragana
AU  - Ilijević, Konstantin
AU  - Vukašinović, Elvira L.
AU  - Blagojević, Duško P.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3734
AB  - Over the past decades, the number of managed honey bee Apis mellifera L. (Hymenoptera: Apidae) colonies have been decreasing. The majority of losses occur during winter, suggesting that overwintering honey bees are more susceptible to adverse factors. We focused on the oxidative status of overwintering honey bees, particularly at the beginning (November) and end (March) of the wintering period. Colonies from three locations with different anthropogenic influences were selected: Belgrade, an urban zone, Zajača, an industrial zone, and Susek, a rural area. We measured levels of malondialdehyde (MDA), as a marker of lipid peroxidation, as well as the expression and activity of select antioxidative enzymes: Superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). Our results show that enzyme activity and gene expression of antioxidative enzymes are influenced by both sample location and the time of sampling. The majority of analyzed genes had significantly reduced expression, at the end of the overwintering period when higher activities of antioxidative enzymes were also recorded. Among the analyzed parameters, SOD activity and gene expression of microsomal GST isoforms were more affected by local environmental conditions, suggesting the complex role of these enzymes in antioxidative defense and detoxification. The higher MDA levels observed at the end of overwintering for all three locations likely reflects accumulated oxidative damage which could be associated with the aging process, brood rearing and/or the onset flying activity.
PB  - E. Schweizerbart'sche Verlagsbuchhandlung
T2  - Entomologia Generalis
T1  - Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees
VL  - 39
IS  - 1
SP  - 33
EP  - 44
DO  - 10.1127/entomologia/2019/0743
ER  - 

@article{
author = "Kojić, Danijela and Purać, Jelena and Nikolić, Tatjana V. and Orčić, Snežana M. and Vujanović, Dragana and Ilijević, Konstantin and Vukašinović, Elvira L. and Blagojević, Duško P.",
year = "2019",
abstract = "Over the past decades, the number of managed honey bee Apis mellifera L. (Hymenoptera: Apidae) colonies have been decreasing. The majority of losses occur during winter, suggesting that overwintering honey bees are more susceptible to adverse factors. We focused on the oxidative status of overwintering honey bees, particularly at the beginning (November) and end (March) of the wintering period. Colonies from three locations with different anthropogenic influences were selected: Belgrade, an urban zone, Zajača, an industrial zone, and Susek, a rural area. We measured levels of malondialdehyde (MDA), as a marker of lipid peroxidation, as well as the expression and activity of select antioxidative enzymes: Superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). Our results show that enzyme activity and gene expression of antioxidative enzymes are influenced by both sample location and the time of sampling. The majority of analyzed genes had significantly reduced expression, at the end of the overwintering period when higher activities of antioxidative enzymes were also recorded. Among the analyzed parameters, SOD activity and gene expression of microsomal GST isoforms were more affected by local environmental conditions, suggesting the complex role of these enzymes in antioxidative defense and detoxification. The higher MDA levels observed at the end of overwintering for all three locations likely reflects accumulated oxidative damage which could be associated with the aging process, brood rearing and/or the onset flying activity.",
publisher = "E. Schweizerbart'sche Verlagsbuchhandlung",
journal = "Entomologia Generalis",
title = "Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees",
volume = "39",
number = "1",
pages = "33-44",
doi = "10.1127/entomologia/2019/0743"
}

Kojić, D., Purać, J., Nikolić, T. V., Orčić, S. M., Vujanović, D., Ilijević, K., Vukašinović, E. L.,& Blagojević, D. P.. (2019). Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees. in Entomologia Generalis
E. Schweizerbart'sche Verlagsbuchhandlung., 39(1), 33-44.
https://doi.org/10.1127/entomologia/2019/0743

Kojić D, Purać J, Nikolić TV, Orčić SM, Vujanović D, Ilijević K, Vukašinović EL, Blagojević DP. Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees. in Entomologia Generalis. 2019;39(1):33-44.
doi:10.1127/entomologia/2019/0743 .

Kojić, Danijela, Purać, Jelena, Nikolić, Tatjana V., Orčić, Snežana M., Vujanović, Dragana, Ilijević, Konstantin, Vukašinović, Elvira L., Blagojević, Duško P., "Oxidative stress and the activity of antioxidative defense enzymes in overwintering honey bees" in Entomologia Generalis, 39, no. 1 (2019):33-44,
https://doi.org/10.1127/entomologia/2019/0743 . .

TY  - CONF
AU  - Orlić, Jovana
AU  - Ilijević, Konstantin
AU  - Savić, Slađana D.
AU  - Zarić, Nenad M.
AU  - Gržetić, Ivan
PY  - 2018
UR  - https://emec19.sciencesconf.org/data/pages/EMEC_19_Book_of_abstract.pdf
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5919
UR  - http://www.europeanace.com/about/meetings
UR  - https://emec19.sciencesconf.org
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5920
AB  - Plants are known as good biomonitors in contaminated areas due to their tendency to accumulate toxic metals. Methods based on direct solid sample analysis are more favourable because they avoid utilization of aggressive and toxic agents, which is in line with green chemistry principles [1]. Besides nondestructiveness, X-ray fluorescence spectroscopy (XRF) is suitable for plant analysis because it offers wide linearity range (from ppm level to 100 %) and possibility of analysis of almost whole PSE (from Be to Am) [2]. Plant sample preparation procedure for WDXRF analysis includes grinding and drying at 60 C. After obtaining a homogeneous mixture, the plant sample is mixed with a certain amount of binder (Hoechst wax C micropowder) and pressed in a hydraulic press (Retsch PP 25) in order to obtain stable pellet (32 mm diameter). The plants are mostly made of light elements (O, N and C) which are transparent for X-rays. During analysis of elements with a higher atomic number, Xrays penetrate quite deep into the sample. It is important to establish the minimum thickness of the pellet that will provide reliable results during determination of the heavier elements in the plant matrix. Samples are measured under conditions of high vacuum and slightly elevated temperature, and for that reason herbal matrix is prone to physical changes after analysis. It is important to determine ideal ratio between mass of the sample and the binder that will provide a stable pellet without affecting determination of elements which are present in low concentrations. During this study, two types of plant samples were analysed: fir and pine needles. Samples were collected in 2017 during autumn. The influence of pellet mass (thickness) on elements concentration was examined by measuring pellets prepared from 1, 2, 3, 4 or 5 g of plans material. By preparing pellets with: 0, 5, 10, 15, 20 and 25 % of wax, the influence of binder ratio was examined. Analysis was performed on ARL™ PERFORM’X Sequential Wavelength Dispersive XRay Fluorescence Spectrometer (Thermo Fisher Scientific, Switzerland) combined with ARL software program UniQuant [3]. In both types of plant samples the following elements were determined: Na, Mg, Al, Si, P, S, Cl, K, Ca, Ti, Mn, Fe, Ni, Zn, Sr and Zr. Both fir and pine needles show similar trends. As quantity of binder increases, the concentration of Ca and K increases, because wax as binder can contain small quantities of those elements. Repeatability of elements that were found in higher concentrations (Mg, Al, Si, P, S, Cl, K and Ca) is high, while repeatability for low concentration elements (Ti, Fe, Ni, Zn, Sr) decreases as percent of binder increases. Reason for that phenomenon is that dilution with wax is affecting elements in low concentration more than high concentration elements. For light elements results show small impact of pellet mass on the measurements because observed X-rays have low penetration depth. Elements with higher Z number are usually present in smaller concentrations in plants and such measurements are less precise, especially when the smaller pellet mass is analyzed. We can say that UniQuant, as standardless method of analysis, which uses the advanced Fundamental parameters Algorithms for data processing, is well adjusted and able to deal with analysis of different sample masses. When physical properties of pellets are investigated, addition of 20% of wax provides the most stabile pellets with flattest surface. For adequate pellet stability, recommend mass of pellets should be 4 g, but it has been shown that 3 g is acceptable in the case of a small sample quantity. In case when we have less than 3 g, it is recommended to carefully place sample as thin layer on the top of 3 g of boric acid inert carrier.
PB  - Institute of Chemistry of Clermont-Ferrand (ICCF) from the Clermont Auvergne University
PB  - CNRS
PB  - Sigma-Clermont
C3  - 19th European Meeting on Environmental Chemistry, Royat, France, 4-6th December
T1  - Optimization of the plant sample preparation procedure for metal analysis using Wavelength Dispersive X-Ray Spectroscopy (WDXRF)
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5920
ER  - 

@conference{
author = "Orlić, Jovana and Ilijević, Konstantin and Savić, Slađana D. and Zarić, Nenad M. and Gržetić, Ivan",
year = "2018",
abstract = "Plants are known as good biomonitors in contaminated areas due to their tendency to accumulate toxic metals. Methods based on direct solid sample analysis are more favourable because they avoid utilization of aggressive and toxic agents, which is in line with green chemistry principles [1]. Besides nondestructiveness, X-ray fluorescence spectroscopy (XRF) is suitable for plant analysis because it offers wide linearity range (from ppm level to 100 %) and possibility of analysis of almost whole PSE (from Be to Am) [2]. Plant sample preparation procedure for WDXRF analysis includes grinding and drying at 60 C. After obtaining a homogeneous mixture, the plant sample is mixed with a certain amount of binder (Hoechst wax C micropowder) and pressed in a hydraulic press (Retsch PP 25) in order to obtain stable pellet (32 mm diameter). The plants are mostly made of light elements (O, N and C) which are transparent for X-rays. During analysis of elements with a higher atomic number, Xrays penetrate quite deep into the sample. It is important to establish the minimum thickness of the pellet that will provide reliable results during determination of the heavier elements in the plant matrix. Samples are measured under conditions of high vacuum and slightly elevated temperature, and for that reason herbal matrix is prone to physical changes after analysis. It is important to determine ideal ratio between mass of the sample and the binder that will provide a stable pellet without affecting determination of elements which are present in low concentrations. During this study, two types of plant samples were analysed: fir and pine needles. Samples were collected in 2017 during autumn. The influence of pellet mass (thickness) on elements concentration was examined by measuring pellets prepared from 1, 2, 3, 4 or 5 g of plans material. By preparing pellets with: 0, 5, 10, 15, 20 and 25 % of wax, the influence of binder ratio was examined. Analysis was performed on ARL™ PERFORM’X Sequential Wavelength Dispersive XRay Fluorescence Spectrometer (Thermo Fisher Scientific, Switzerland) combined with ARL software program UniQuant [3]. In both types of plant samples the following elements were determined: Na, Mg, Al, Si, P, S, Cl, K, Ca, Ti, Mn, Fe, Ni, Zn, Sr and Zr. Both fir and pine needles show similar trends. As quantity of binder increases, the concentration of Ca and K increases, because wax as binder can contain small quantities of those elements. Repeatability of elements that were found in higher concentrations (Mg, Al, Si, P, S, Cl, K and Ca) is high, while repeatability for low concentration elements (Ti, Fe, Ni, Zn, Sr) decreases as percent of binder increases. Reason for that phenomenon is that dilution with wax is affecting elements in low concentration more than high concentration elements. For light elements results show small impact of pellet mass on the measurements because observed X-rays have low penetration depth. Elements with higher Z number are usually present in smaller concentrations in plants and such measurements are less precise, especially when the smaller pellet mass is analyzed. We can say that UniQuant, as standardless method of analysis, which uses the advanced Fundamental parameters Algorithms for data processing, is well adjusted and able to deal with analysis of different sample masses. When physical properties of pellets are investigated, addition of 20% of wax provides the most stabile pellets with flattest surface. For adequate pellet stability, recommend mass of pellets should be 4 g, but it has been shown that 3 g is acceptable in the case of a small sample quantity. In case when we have less than 3 g, it is recommended to carefully place sample as thin layer on the top of 3 g of boric acid inert carrier.",
publisher = "Institute of Chemistry of Clermont-Ferrand (ICCF) from the Clermont Auvergne University, CNRS, Sigma-Clermont",
journal = "19th European Meeting on Environmental Chemistry, Royat, France, 4-6th December",
title = "Optimization of the plant sample preparation procedure for metal analysis using Wavelength Dispersive X-Ray Spectroscopy (WDXRF)",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5920"
}

Orlić, J., Ilijević, K., Savić, S. D., Zarić, N. M.,& Gržetić, I.. (2018). Optimization of the plant sample preparation procedure for metal analysis using Wavelength Dispersive X-Ray Spectroscopy (WDXRF). in 19th European Meeting on Environmental Chemistry, Royat, France, 4-6th December
Institute of Chemistry of Clermont-Ferrand (ICCF) from the Clermont Auvergne University..
https://hdl.handle.net/21.15107/rcub_cherry_5920

Orlić J, Ilijević K, Savić SD, Zarić NM, Gržetić I. Optimization of the plant sample preparation procedure for metal analysis using Wavelength Dispersive X-Ray Spectroscopy (WDXRF). in 19th European Meeting on Environmental Chemistry, Royat, France, 4-6th December. 2018;.
https://hdl.handle.net/21.15107/rcub_cherry_5920 .

Orlić, Jovana, Ilijević, Konstantin, Savić, Slađana D., Zarić, Nenad M., Gržetić, Ivan, "Optimization of the plant sample preparation procedure for metal analysis using Wavelength Dispersive X-Ray Spectroscopy (WDXRF)" in 19th European Meeting on Environmental Chemistry, Royat, France, 4-6th December (2018),
https://hdl.handle.net/21.15107/rcub_cherry_5920 .

TY  - CONF
AU  - Orlić, Jovana
AU  - Ilijević, Konstantin
AU  - Savić, Slađana D.
AU  - Zarić, Nenad M.
AU  - Gržetić, Ivan
PY  - 2018
UR  - https://emec19.sciencesconf.org/data/pages/EMEC_19_Book_of_abstract.pdf
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5919
UR  - https://emec19.sciencesconf.org
UR  - http://www.europeanace.com/about/meetings
AB  - Plants are known as good biomonitors in contaminated areas due to their tendency to accumulate toxic metals. Methods based on direct solid sample analysis are more favourable because they avoid utilization of aggressive and toxic agents, which is in line with green chemistry principles [1]. Besides nondestructiveness, X-ray fluorescence spectroscopy (XRF) is suitable for plant analysis because it offers wide linearity range (from ppm level to 100 %) and possibility of analysis of almost whole PSE (from Be to Am) [2]. 
Plant sample preparation procedure for WDXRF analysis includes grinding and drying at 60 C. After obtaining a homogeneous mixture, the plant sample is mixed with a certain amount of binder (Hoechst wax C micropowder) and pressed in a hydraulic press (Retsch PP 25) in order to obtain stable pellet (32 mm diameter). 
The plants are mostly made of light elements (O, N and C) which are transparent for X-rays. During analysis of elements with a higher atomic number, Xrays penetrate quite deep into the sample. It is important to establish the minimum thickness of the pellet that will provide reliable results during determination of the heavier elements in the plant matrix. Samples are measured under conditions of high vacuum and slightly elevated temperature, and for that reason herbal matrix is prone to physical changes after analysis. It is important to determine ideal ratio between mass of the sample and the binder that will provide a stable pellet without affecting determination of elements which are present in low concentrations. 
During this study, two types of plant samples were analysed: fir and pine needles. Samples were collected in 2017 during autumn. The influence of pellet mass (thickness) on elements concentration was examined by measuring pellets prepared from 1, 2, 3, 4 or 5 g of plans material. By preparing pellets with: 0, 5, 10, 15, 20 and 25 % of wax, the influence of binder ratio was examined. Analysis was performed on ARL™ PERFORM’X Sequential Wavelength Dispersive XRay Fluorescence Spectrometer (Thermo Fisher Scientific, Switzerland) combined with ARL software program UniQuant [3]. 
In both types of plant samples the following elements were determined: Na, Mg, Al, Si, P, S, Cl, K, Ca, Ti, Mn, Fe, Ni, Zn, Sr and Zr. Both fir and pine needles show similar trends. As quantity of binder increases, the concentration of Ca and K increases, because wax as binder can contain small quantities of those elements. Repeatability of elements that were found in higher concentrations (Mg, Al, Si, P, S, Cl, K and Ca) is high, while repeatability for low concentration elements (Ti, Fe, Ni, Zn, Sr) decreases as percent of binder increases. Reason for that phenomenon is that dilution with wax is affecting elements in low concentration more than high concentration elements. For light elements results show small impact of pellet mass on the measurements because observed X-rays have low penetration depth. Elements with higher Z number are usually present in smaller concentrations in plants and such measurements are less precise, especially when the smaller pellet mass is analyzed. We can say that UniQuant, as standardless method of analysis, which uses the advanced Fundamental parameters Algorithms for data processing, is well adjusted and able to deal with analysis of different sample masses. 
When physical properties of pellets are investigated, addition of 20% of wax provides the most stabile pellets with flattest surface. For adequate pellet stability, recommend mass of pellets should be 4 g, but it has been shown that 3 g is acceptable in the case of a small sample quantity. In case when we have less than 3 g, it is recommended to carefully place sample as thin layer on the top of 3 g of boric acid inert carrier.
PB  - Institute of Chemistry of Clermont-Ferrand (ICCF) from the Clermont Auvergne University
PB  - CNRS
PB  - Sigma-Clermont
C3  - 19th European Meeting on Environmental Chemistry, Royat, France, 4-6th December
T1  - Optimization of the plant sample preparation procedure for metal analysis using Wavelength Dispersive X-Ray Spectroscopy (WDXRF)
IS  - 19
SP  - 134
EP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5919
ER  - 

@conference{
author = "Orlić, Jovana and Ilijević, Konstantin and Savić, Slađana D. and Zarić, Nenad M. and Gržetić, Ivan",
year = "2018",
abstract = "Plants are known as good biomonitors in contaminated areas due to their tendency to accumulate toxic metals. Methods based on direct solid sample analysis are more favourable because they avoid utilization of aggressive and toxic agents, which is in line with green chemistry principles [1]. Besides nondestructiveness, X-ray fluorescence spectroscopy (XRF) is suitable for plant analysis because it offers wide linearity range (from ppm level to 100 %) and possibility of analysis of almost whole PSE (from Be to Am) [2]. 
Plant sample preparation procedure for WDXRF analysis includes grinding and drying at 60 C. After obtaining a homogeneous mixture, the plant sample is mixed with a certain amount of binder (Hoechst wax C micropowder) and pressed in a hydraulic press (Retsch PP 25) in order to obtain stable pellet (32 mm diameter). 
The plants are mostly made of light elements (O, N and C) which are transparent for X-rays. During analysis of elements with a higher atomic number, Xrays penetrate quite deep into the sample. It is important to establish the minimum thickness of the pellet that will provide reliable results during determination of the heavier elements in the plant matrix. Samples are measured under conditions of high vacuum and slightly elevated temperature, and for that reason herbal matrix is prone to physical changes after analysis. It is important to determine ideal ratio between mass of the sample and the binder that will provide a stable pellet without affecting determination of elements which are present in low concentrations. 
During this study, two types of plant samples were analysed: fir and pine needles. Samples were collected in 2017 during autumn. The influence of pellet mass (thickness) on elements concentration was examined by measuring pellets prepared from 1, 2, 3, 4 or 5 g of plans material. By preparing pellets with: 0, 5, 10, 15, 20 and 25 % of wax, the influence of binder ratio was examined. Analysis was performed on ARL™ PERFORM’X Sequential Wavelength Dispersive XRay Fluorescence Spectrometer (Thermo Fisher Scientific, Switzerland) combined with ARL software program UniQuant [3]. 
In both types of plant samples the following elements were determined: Na, Mg, Al, Si, P, S, Cl, K, Ca, Ti, Mn, Fe, Ni, Zn, Sr and Zr. Both fir and pine needles show similar trends. As quantity of binder increases, the concentration of Ca and K increases, because wax as binder can contain small quantities of those elements. Repeatability of elements that were found in higher concentrations (Mg, Al, Si, P, S, Cl, K and Ca) is high, while repeatability for low concentration elements (Ti, Fe, Ni, Zn, Sr) decreases as percent of binder increases. Reason for that phenomenon is that dilution with wax is affecting elements in low concentration more than high concentration elements. For light elements results show small impact of pellet mass on the measurements because observed X-rays have low penetration depth. Elements with higher Z number are usually present in smaller concentrations in plants and such measurements are less precise, especially when the smaller pellet mass is analyzed. We can say that UniQuant, as standardless method of analysis, which uses the advanced Fundamental parameters Algorithms for data processing, is well adjusted and able to deal with analysis of different sample masses. 
When physical properties of pellets are investigated, addition of 20% of wax provides the most stabile pellets with flattest surface. For adequate pellet stability, recommend mass of pellets should be 4 g, but it has been shown that 3 g is acceptable in the case of a small sample quantity. In case when we have less than 3 g, it is recommended to carefully place sample as thin layer on the top of 3 g of boric acid inert carrier.",
publisher = "Institute of Chemistry of Clermont-Ferrand (ICCF) from the Clermont Auvergne University, CNRS, Sigma-Clermont",
journal = "19th European Meeting on Environmental Chemistry, Royat, France, 4-6th December",
title = "Optimization of the plant sample preparation procedure for metal analysis using Wavelength Dispersive X-Ray Spectroscopy (WDXRF)",
number = "19",
pages = "134-134",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5919"
}

Orlić, J., Ilijević, K., Savić, S. D., Zarić, N. M.,& Gržetić, I.. (2018). Optimization of the plant sample preparation procedure for metal analysis using Wavelength Dispersive X-Ray Spectroscopy (WDXRF). in 19th European Meeting on Environmental Chemistry, Royat, France, 4-6th December
Institute of Chemistry of Clermont-Ferrand (ICCF) from the Clermont Auvergne University.(19), 134-134.
https://hdl.handle.net/21.15107/rcub_cherry_5919

Orlić J, Ilijević K, Savić SD, Zarić NM, Gržetić I. Optimization of the plant sample preparation procedure for metal analysis using Wavelength Dispersive X-Ray Spectroscopy (WDXRF). in 19th European Meeting on Environmental Chemistry, Royat, France, 4-6th December. 2018;(19):134-134.
https://hdl.handle.net/21.15107/rcub_cherry_5919 .

Orlić, Jovana, Ilijević, Konstantin, Savić, Slađana D., Zarić, Nenad M., Gržetić, Ivan, "Optimization of the plant sample preparation procedure for metal analysis using Wavelength Dispersive X-Ray Spectroscopy (WDXRF)" in 19th European Meeting on Environmental Chemistry, Royat, France, 4-6th December, no. 19 (2018):134-134,
https://hdl.handle.net/21.15107/rcub_cherry_5919 .

TY  - JOUR
AU  - Zarić, Nenad M.
AU  - Ilijević, Konstantin
AU  - Stanisavljević, Ljubiša
AU  - Gržetić, Ivan
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2179
AB  - Honeybees have been proposed and used as bioindicators for the last few decades, because of their nature. Until now they have mostly been used to determine the present pollution and to distinguish the differences between the sampling locations and the sampling periods. With the use of multivariate statistical methods honeybees can also be used to distinguish the origin of this pollution. In this study the concentrations of Al, Ba, Ca, Cd, Co, Cr, Cu, Fe, Mg, Mn, Na, Ni, Sr and Zn were measured in the bodies of adult honeybees collected from nine different apiaries in Serbia. With the help of the statistical methods it was established that the least polluted area was the one that has no industrial activities or the intense traffic nearby. The most polluted was the urban region, followed by a region close to thermal power plants and ash disposal site. Using PCA and CA the origin of the analyzed metals were proposed. It was suggested that Al, Ba, Ca, Cd, Co, Cr, Cu, Fe, Mn and Ni have anthropogenic origin mainly from the intensive agriculture, traffic and coal combustion.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Use of honeybees (Apis mellifera L.) as bioindicators of spatial variations and origin determination of metal pollution in Serbia
VL  - 83
IS  - 6
SP  - 773
EP  - 784
DO  - 10.2298/JSC171110018Z
ER  - 

@article{
author = "Zarić, Nenad M. and Ilijević, Konstantin and Stanisavljević, Ljubiša and Gržetić, Ivan",
year = "2018",
abstract = "Honeybees have been proposed and used as bioindicators for the last few decades, because of their nature. Until now they have mostly been used to determine the present pollution and to distinguish the differences between the sampling locations and the sampling periods. With the use of multivariate statistical methods honeybees can also be used to distinguish the origin of this pollution. In this study the concentrations of Al, Ba, Ca, Cd, Co, Cr, Cu, Fe, Mg, Mn, Na, Ni, Sr and Zn were measured in the bodies of adult honeybees collected from nine different apiaries in Serbia. With the help of the statistical methods it was established that the least polluted area was the one that has no industrial activities or the intense traffic nearby. The most polluted was the urban region, followed by a region close to thermal power plants and ash disposal site. Using PCA and CA the origin of the analyzed metals were proposed. It was suggested that Al, Ba, Ca, Cd, Co, Cr, Cu, Fe, Mn and Ni have anthropogenic origin mainly from the intensive agriculture, traffic and coal combustion.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Use of honeybees (Apis mellifera L.) as bioindicators of spatial variations and origin determination of metal pollution in Serbia",
volume = "83",
number = "6",
pages = "773-784",
doi = "10.2298/JSC171110018Z"
}

Zarić, N. M., Ilijević, K., Stanisavljević, L.,& Gržetić, I.. (2018). Use of honeybees (Apis mellifera L.) as bioindicators of spatial variations and origin determination of metal pollution in Serbia. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 83(6), 773-784.
https://doi.org/10.2298/JSC171110018Z

Zarić NM, Ilijević K, Stanisavljević L, Gržetić I. Use of honeybees (Apis mellifera L.) as bioindicators of spatial variations and origin determination of metal pollution in Serbia. in Journal of the Serbian Chemical Society. 2018;83(6):773-784.
doi:10.2298/JSC171110018Z .

Zarić, Nenad M., Ilijević, Konstantin, Stanisavljević, Ljubiša, Gržetić, Ivan, "Use of honeybees (Apis mellifera L.) as bioindicators of spatial variations and origin determination of metal pollution in Serbia" in Journal of the Serbian Chemical Society, 83, no. 6 (2018):773-784,
https://doi.org/10.2298/JSC171110018Z . .

TY  - JOUR
AU  - Zarić, Nenad M.
AU  - Deljanin, Isidora
AU  - Ilijević, Konstantin
AU  - Stanisavljević, Ljubiša
AU  - Ristić, Mirjana
AU  - Gržetić, Ivan
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2183
AB  - With the increase in anthropogenic activities metal pollution is also increased and needs to be closely monitored. In this study honeybees were used as bioindicators to monitor metal pollution. Metal pollution in honeybees represents pollution present in air, water and soil. Concentrations of As, Cs, Hg, Mo, Sb, Se, U and V were measured. The aim of this study was to assess spatial and temporal variations of metal concentrations in honeybees. Samples of honeybees were taken at five different regions in Serbia (Belgrade - BG, Pancevo - PA, Pavlis - PV, Mesic - MS, and Kostolac - TPP) during 2014. Spatial variations were observed for Sb, which had higher concentrations in BG compared to all other regions, and for U, with higher concentrations in the TPP region. High concentrations of Sb in BG were attributed to intense traffic, while higher U concentrations in the TPP region are due to the vicinity of coal fired power plants. In order to assess temporal variations at two locations (PA and PV) samples were taken during July and September of 2014 and June, July, August and September of 2015. During 2014 observing months of sampling higher concentrations in July were detected for Sb and U in BG, which is attributed to lifecycle of plants and honeybees. During the same year higher concentrations in September were observed for As, Sb in PA and Hg in PV. This is due to high precipitation during the peak of bee activity in spring/summer of 2014. No differences between months of sampling were detected during 2015. Between 2014 and 2015 statistically significant differences were observed for Hg, Mo and V; all elements had higher concentrations in 2014. This is in accordance with the trend of reduction of metal concentrations in the bodies of honeybees throughout the years in this region.
PB  - Peerj Inc, London
T2  - PEERJ
T1  - Assessment of spatial and temporal variations in trace element concentrations using honeybees (Apis mellifera) as bioindicators
VL  - 6
DO  - 10.7717/peerj.5197
ER  - 

@article{
author = "Zarić, Nenad M. and Deljanin, Isidora and Ilijević, Konstantin and Stanisavljević, Ljubiša and Ristić, Mirjana and Gržetić, Ivan",
year = "2018",
abstract = "With the increase in anthropogenic activities metal pollution is also increased and needs to be closely monitored. In this study honeybees were used as bioindicators to monitor metal pollution. Metal pollution in honeybees represents pollution present in air, water and soil. Concentrations of As, Cs, Hg, Mo, Sb, Se, U and V were measured. The aim of this study was to assess spatial and temporal variations of metal concentrations in honeybees. Samples of honeybees were taken at five different regions in Serbia (Belgrade - BG, Pancevo - PA, Pavlis - PV, Mesic - MS, and Kostolac - TPP) during 2014. Spatial variations were observed for Sb, which had higher concentrations in BG compared to all other regions, and for U, with higher concentrations in the TPP region. High concentrations of Sb in BG were attributed to intense traffic, while higher U concentrations in the TPP region are due to the vicinity of coal fired power plants. In order to assess temporal variations at two locations (PA and PV) samples were taken during July and September of 2014 and June, July, August and September of 2015. During 2014 observing months of sampling higher concentrations in July were detected for Sb and U in BG, which is attributed to lifecycle of plants and honeybees. During the same year higher concentrations in September were observed for As, Sb in PA and Hg in PV. This is due to high precipitation during the peak of bee activity in spring/summer of 2014. No differences between months of sampling were detected during 2015. Between 2014 and 2015 statistically significant differences were observed for Hg, Mo and V; all elements had higher concentrations in 2014. This is in accordance with the trend of reduction of metal concentrations in the bodies of honeybees throughout the years in this region.",
publisher = "Peerj Inc, London",
journal = "PEERJ",
title = "Assessment of spatial and temporal variations in trace element concentrations using honeybees (Apis mellifera) as bioindicators",
volume = "6",
doi = "10.7717/peerj.5197"
}

Zarić, N. M., Deljanin, I., Ilijević, K., Stanisavljević, L., Ristić, M.,& Gržetić, I.. (2018). Assessment of spatial and temporal variations in trace element concentrations using honeybees (Apis mellifera) as bioindicators. in PEERJ
Peerj Inc, London., 6.
https://doi.org/10.7717/peerj.5197

Zarić NM, Deljanin I, Ilijević K, Stanisavljević L, Ristić M, Gržetić I. Assessment of spatial and temporal variations in trace element concentrations using honeybees (Apis mellifera) as bioindicators. in PEERJ. 2018;6.
doi:10.7717/peerj.5197 .

Zarić, Nenad M., Deljanin, Isidora, Ilijević, Konstantin, Stanisavljević, Ljubiša, Ristić, Mirjana, Gržetić, Ivan, "Assessment of spatial and temporal variations in trace element concentrations using honeybees (Apis mellifera) as bioindicators" in PEERJ, 6 (2018),
https://doi.org/10.7717/peerj.5197 . .

TY  - JOUR
AU  - Zarić, Nenad M.
AU  - Deljanin, Isidora
AU  - Ilijević, Konstantin
AU  - Stanisavljević, Ljubiša
AU  - Ristić, Mirjana
AU  - Gržetić, Ivan
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2187
AB  - In this study, honeybees were used to determine spatio-temporal variations and origin sources of Pb. Lead concentrations and isotopic composition were used in combination with selected statistical methods. The sampling was carried out at five different locations in Serbia: urban region (BG), petrochemical industry (PA), suburban region (PV), rural region (MS) and thermal power plant region (TPP) during 2014. At PA and PV locations, samples were taken during multiple years. This is the first use of Kohonen self-organizing map (SOM) in combination with honeybees as bioindicators to determine spatio-temporal variations and origin of Pb pollution. It was observed that during the years Pb concentrations were in decline. Anthropogenic sources are most dominant in BG and TPP, in PA there are mixed sources of natural and anthropogenic origin and in PV Pb is of natural origin. It can be concluded that honeybees in combination with SOM can be used to differentiate between slight changes in spatio-temporal variations of Pb, as well as for source appointment. (C) 2018 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Science of the Total Environment
T1  - Honeybees as sentinels of lead pollution: Spatio-temporal variations and source appointment using stable isotopes and Kohonen self-organizing maps
VL  - 642
SP  - 56
EP  - 62
DO  - 10.1016/j.scitotenv.2018.06.040
ER  - 

@article{
author = "Zarić, Nenad M. and Deljanin, Isidora and Ilijević, Konstantin and Stanisavljević, Ljubiša and Ristić, Mirjana and Gržetić, Ivan",
year = "2018",
abstract = "In this study, honeybees were used to determine spatio-temporal variations and origin sources of Pb. Lead concentrations and isotopic composition were used in combination with selected statistical methods. The sampling was carried out at five different locations in Serbia: urban region (BG), petrochemical industry (PA), suburban region (PV), rural region (MS) and thermal power plant region (TPP) during 2014. At PA and PV locations, samples were taken during multiple years. This is the first use of Kohonen self-organizing map (SOM) in combination with honeybees as bioindicators to determine spatio-temporal variations and origin of Pb pollution. It was observed that during the years Pb concentrations were in decline. Anthropogenic sources are most dominant in BG and TPP, in PA there are mixed sources of natural and anthropogenic origin and in PV Pb is of natural origin. It can be concluded that honeybees in combination with SOM can be used to differentiate between slight changes in spatio-temporal variations of Pb, as well as for source appointment. (C) 2018 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Science of the Total Environment",
title = "Honeybees as sentinels of lead pollution: Spatio-temporal variations and source appointment using stable isotopes and Kohonen self-organizing maps",
volume = "642",
pages = "56-62",
doi = "10.1016/j.scitotenv.2018.06.040"
}

Zarić, N. M., Deljanin, I., Ilijević, K., Stanisavljević, L., Ristić, M.,& Gržetić, I.. (2018). Honeybees as sentinels of lead pollution: Spatio-temporal variations and source appointment using stable isotopes and Kohonen self-organizing maps. in Science of the Total Environment
Elsevier Science Bv, Amsterdam., 642, 56-62.
https://doi.org/10.1016/j.scitotenv.2018.06.040

Zarić NM, Deljanin I, Ilijević K, Stanisavljević L, Ristić M, Gržetić I. Honeybees as sentinels of lead pollution: Spatio-temporal variations and source appointment using stable isotopes and Kohonen self-organizing maps. in Science of the Total Environment. 2018;642:56-62.
doi:10.1016/j.scitotenv.2018.06.040 .

Zarić, Nenad M., Deljanin, Isidora, Ilijević, Konstantin, Stanisavljević, Ljubiša, Ristić, Mirjana, Gržetić, Ivan, "Honeybees as sentinels of lead pollution: Spatio-temporal variations and source appointment using stable isotopes and Kohonen self-organizing maps" in Science of the Total Environment, 642 (2018):56-62,
https://doi.org/10.1016/j.scitotenv.2018.06.040 . .

TY  - DATA
AU  - Zarić, Nenad M.
AU  - Deljanin, Isidora
AU  - Ilijević, Konstantin
AU  - Stanisavljević, Ljubiša
AU  - Ristić, Mirjana
AU  - Gržetić, Ivan
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3174
PB  - Peerj Inc, London
T2  - PEERJ
T1  - Supplementary material for the article: Zaric, N. M.; Deljanin, I.; Ilijević, K.; Stanisavljević, L.; Ristić, M.; Gržetić, I. Assessment of  Spatial and Temporal Variations in Trace Element Concentrations Using Honeybees (Apis  Mellifera) as Bioindicators. PeerJ 2018, 2018 (7). https://doi.org/10.7717/peerj.5197
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3174
ER  - 

@misc{
author = "Zarić, Nenad M. and Deljanin, Isidora and Ilijević, Konstantin and Stanisavljević, Ljubiša and Ristić, Mirjana and Gržetić, Ivan",
year = "2018",
publisher = "Peerj Inc, London",
journal = "PEERJ",
title = "Supplementary material for the article: Zaric, N. M.; Deljanin, I.; Ilijević, K.; Stanisavljević, L.; Ristić, M.; Gržetić, I. Assessment of  Spatial and Temporal Variations in Trace Element Concentrations Using Honeybees (Apis  Mellifera) as Bioindicators. PeerJ 2018, 2018 (7). https://doi.org/10.7717/peerj.5197",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3174"
}

Zarić, N. M., Deljanin, I., Ilijević, K., Stanisavljević, L., Ristić, M.,& Gržetić, I.. (2018). Supplementary material for the article: Zaric, N. M.; Deljanin, I.; Ilijević, K.; Stanisavljević, L.; Ristić, M.; Gržetić, I. Assessment of  Spatial and Temporal Variations in Trace Element Concentrations Using Honeybees (Apis  Mellifera) as Bioindicators. PeerJ 2018, 2018 (7). https://doi.org/10.7717/peerj.5197. in PEERJ
Peerj Inc, London..
https://hdl.handle.net/21.15107/rcub_cherry_3174

Zarić NM, Deljanin I, Ilijević K, Stanisavljević L, Ristić M, Gržetić I. Supplementary material for the article: Zaric, N. M.; Deljanin, I.; Ilijević, K.; Stanisavljević, L.; Ristić, M.; Gržetić, I. Assessment of  Spatial and Temporal Variations in Trace Element Concentrations Using Honeybees (Apis  Mellifera) as Bioindicators. PeerJ 2018, 2018 (7). https://doi.org/10.7717/peerj.5197. in PEERJ. 2018;.
https://hdl.handle.net/21.15107/rcub_cherry_3174 .

Zarić, Nenad M., Deljanin, Isidora, Ilijević, Konstantin, Stanisavljević, Ljubiša, Ristić, Mirjana, Gržetić, Ivan, "Supplementary material for the article: Zaric, N. M.; Deljanin, I.; Ilijević, K.; Stanisavljević, L.; Ristić, M.; Gržetić, I. Assessment of  Spatial and Temporal Variations in Trace Element Concentrations Using Honeybees (Apis  Mellifera) as Bioindicators. PeerJ 2018, 2018 (7). https://doi.org/10.7717/peerj.5197" in PEERJ (2018),
https://hdl.handle.net/21.15107/rcub_cherry_3174 .

TY  - DATA
AU  - Zarić, Nenad M.
AU  - Ilijević, Konstantin
AU  - Stanisavljević, Ljubiša
AU  - Gržetić, Ivan
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3312
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Supplementary data for the article: Zarić, N. M.; Ilijević, K.; Stanisavljević, L.; Gržetić, I. Use of Honeybees (Apis Mellifera L.) as Bioindicators of Spatial Variations and Origin Determination of Metal Pollution in Serbia. Journal of the Serbian Chemical Society 2018, 83 (6), 773–784. https://doi.org/10.2298/JSC171110018Z
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3312
ER  - 

@misc{
author = "Zarić, Nenad M. and Ilijević, Konstantin and Stanisavljević, Ljubiša and Gržetić, Ivan",
year = "2018",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Supplementary data for the article: Zarić, N. M.; Ilijević, K.; Stanisavljević, L.; Gržetić, I. Use of Honeybees (Apis Mellifera L.) as Bioindicators of Spatial Variations and Origin Determination of Metal Pollution in Serbia. Journal of the Serbian Chemical Society 2018, 83 (6), 773–784. https://doi.org/10.2298/JSC171110018Z",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3312"
}

Zarić, N. M., Ilijević, K., Stanisavljević, L.,& Gržetić, I.. (2018). Supplementary data for the article: Zarić, N. M.; Ilijević, K.; Stanisavljević, L.; Gržetić, I. Use of Honeybees (Apis Mellifera L.) as Bioindicators of Spatial Variations and Origin Determination of Metal Pollution in Serbia. Journal of the Serbian Chemical Society 2018, 83 (6), 773–784. https://doi.org/10.2298/JSC171110018Z. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade..
https://hdl.handle.net/21.15107/rcub_cherry_3312

Zarić NM, Ilijević K, Stanisavljević L, Gržetić I. Supplementary data for the article: Zarić, N. M.; Ilijević, K.; Stanisavljević, L.; Gržetić, I. Use of Honeybees (Apis Mellifera L.) as Bioindicators of Spatial Variations and Origin Determination of Metal Pollution in Serbia. Journal of the Serbian Chemical Society 2018, 83 (6), 773–784. https://doi.org/10.2298/JSC171110018Z. in Journal of the Serbian Chemical Society. 2018;.
https://hdl.handle.net/21.15107/rcub_cherry_3312 .

Zarić, Nenad M., Ilijević, Konstantin, Stanisavljević, Ljubiša, Gržetić, Ivan, "Supplementary data for the article: Zarić, N. M.; Ilijević, K.; Stanisavljević, L.; Gržetić, I. Use of Honeybees (Apis Mellifera L.) as Bioindicators of Spatial Variations and Origin Determination of Metal Pollution in Serbia. Journal of the Serbian Chemical Society 2018, 83 (6), 773–784. https://doi.org/10.2298/JSC171110018Z" in Journal of the Serbian Chemical Society (2018),
https://hdl.handle.net/21.15107/rcub_cherry_3312 .

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Jama, Adel
AU  - Jasnić, Nebojša
AU  - Vujovic, Predrag
AU  - Jovanović, Miloš
AU  - Mitić-Ćulafić, Dragana
AU  - Knežević-Vukčević, Jelena
AU  - Cakić Milosević, Maja
AU  - Ilijević, Konstantin
AU  - Popović-Đorđević, Jelena
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2401
AB  - One of the useful properties of probiotic bacteria is their capacity to bind different targets, thus eliminating them through feces. It is supposed that one of these targets could be cadmium, a widespread environmental toxicant that causes various disturbances in biological systems. This study examined the protective effects of probiotic supplementation against cadmium-induced toxicity in the rat. The experiment was conducted in the course of 5 weeks. Animals were divided into four groups: (1) controls, (2) probiotics treated, (3) cadmium treated, and (4) probiotics + cadmium treated. The cadmium concentration was measured in the blood, liver, kidney, and feces, as well as the blood alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as biomarkers of the liver function. Histomorphological changes in the liver and kidney were also determined. Our results revealed that probiotics combined with cadmium increase this metal concentration in feces. As a result, blood, liver, and kidney Cd levels, as well as blood ALT and AST activities were lessened compared to the rat group treated with cadmium only. Besides, probiotics consumed simultaneously with cadmium attenuated histomorphological changes in the liver and kidney caused by cadmium. The rise in lactobacilli number in feces of rats treated simultaneously with cadmium and probiotics results in strong correlation with the increase of Cd concentration in their feces and the decrease of Cd concentration in their blood. We speculate that probiotics actively contribute to cadmium excretion through feces, probably, by its binding to their bacterial cell wall.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Journal of Medicinal Food
T1  - The Protective Effects of Probiotic Bacteria on Cadmium Toxicity in Rats
VL  - 20
IS  - 2
SP  - 189
EP  - 196
DO  - 10.1089/jmf.2016.0090
ER  - 

@article{
author = "Đurašević, Siniša and Jama, Adel and Jasnić, Nebojša and Vujovic, Predrag and Jovanović, Miloš and Mitić-Ćulafić, Dragana and Knežević-Vukčević, Jelena and Cakić Milosević, Maja and Ilijević, Konstantin and Popović-Đorđević, Jelena",
year = "2017",
abstract = "One of the useful properties of probiotic bacteria is their capacity to bind different targets, thus eliminating them through feces. It is supposed that one of these targets could be cadmium, a widespread environmental toxicant that causes various disturbances in biological systems. This study examined the protective effects of probiotic supplementation against cadmium-induced toxicity in the rat. The experiment was conducted in the course of 5 weeks. Animals were divided into four groups: (1) controls, (2) probiotics treated, (3) cadmium treated, and (4) probiotics + cadmium treated. The cadmium concentration was measured in the blood, liver, kidney, and feces, as well as the blood alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as biomarkers of the liver function. Histomorphological changes in the liver and kidney were also determined. Our results revealed that probiotics combined with cadmium increase this metal concentration in feces. As a result, blood, liver, and kidney Cd levels, as well as blood ALT and AST activities were lessened compared to the rat group treated with cadmium only. Besides, probiotics consumed simultaneously with cadmium attenuated histomorphological changes in the liver and kidney caused by cadmium. The rise in lactobacilli number in feces of rats treated simultaneously with cadmium and probiotics results in strong correlation with the increase of Cd concentration in their feces and the decrease of Cd concentration in their blood. We speculate that probiotics actively contribute to cadmium excretion through feces, probably, by its binding to their bacterial cell wall.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Journal of Medicinal Food",
title = "The Protective Effects of Probiotic Bacteria on Cadmium Toxicity in Rats",
volume = "20",
number = "2",
pages = "189-196",
doi = "10.1089/jmf.2016.0090"
}

Đurašević, S., Jama, A., Jasnić, N., Vujovic, P., Jovanović, M., Mitić-Ćulafić, D., Knežević-Vukčević, J., Cakić Milosević, M., Ilijević, K.,& Popović-Đorđević, J.. (2017). The Protective Effects of Probiotic Bacteria on Cadmium Toxicity in Rats. in Journal of Medicinal Food
Mary Ann Liebert, Inc, New Rochelle., 20(2), 189-196.
https://doi.org/10.1089/jmf.2016.0090

Đurašević S, Jama A, Jasnić N, Vujovic P, Jovanović M, Mitić-Ćulafić D, Knežević-Vukčević J, Cakić Milosević M, Ilijević K, Popović-Đorđević J. The Protective Effects of Probiotic Bacteria on Cadmium Toxicity in Rats. in Journal of Medicinal Food. 2017;20(2):189-196.
doi:10.1089/jmf.2016.0090 .

Đurašević, Siniša, Jama, Adel, Jasnić, Nebojša, Vujovic, Predrag, Jovanović, Miloš, Mitić-Ćulafić, Dragana, Knežević-Vukčević, Jelena, Cakić Milosević, Maja, Ilijević, Konstantin, Popović-Đorđević, Jelena, "The Protective Effects of Probiotic Bacteria on Cadmium Toxicity in Rats" in Journal of Medicinal Food, 20, no. 2 (2017):189-196,
https://doi.org/10.1089/jmf.2016.0090 . .