TY  - JOUR
AU  - Nikolić, Jasna
AU  - Nešić, Andrijana N.
AU  - Kull, Skadi
AU  - Schocker, Frauke
AU  - Jappe, Uta
AU  - Gavrović-Jankulović, Marija
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2117
AB  - Diagnostic reagents based on food allergen extracts often lack sufficient sensitivity. The introduction of well characterized food allergens in molecular allergy diagnosis has been recognized as valid approach to circumvent unstandardized allergen extracts. Banana fruit (Musa acuminate) is a well-established allergen source which besides six characterized allergens, contains unidentified IgE reactive proteins whose clinical relevance remains undefined. By employment of a combinatorial peptide ligand library (CPLL) methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis, a novel allergen from banana fruit was detected in banana as catalase. A recombinant homologue of natural catalase was produced, isolated and biochemically characterized. The recombinant protein showed IgE reactivity in 7 out of 13 tested patients with suspected allergy to banana in immunoblot. Novel banana fruit allergens should be added as components to allergen-microarrays for the diagnosis and the monitoring of banana allergy. Significance: By employment of CPLL methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis catalase from banana fruit is identified as a novel allergen, with proposed designation as Mus a 7. IgE reactive recombinant Mus a 7 was produced and should be included in a component-resolved allergy diagnosis.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Proteomics
T1  - Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana
VL  - 175
SP  - 87
EP  - 94
DO  - 10.1016/j.jprot.2018.01.007
ER  - 

@article{
author = "Nikolić, Jasna and Nešić, Andrijana N. and Kull, Skadi and Schocker, Frauke and Jappe, Uta and Gavrović-Jankulović, Marija",
year = "2018",
abstract = "Diagnostic reagents based on food allergen extracts often lack sufficient sensitivity. The introduction of well characterized food allergens in molecular allergy diagnosis has been recognized as valid approach to circumvent unstandardized allergen extracts. Banana fruit (Musa acuminate) is a well-established allergen source which besides six characterized allergens, contains unidentified IgE reactive proteins whose clinical relevance remains undefined. By employment of a combinatorial peptide ligand library (CPLL) methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis, a novel allergen from banana fruit was detected in banana as catalase. A recombinant homologue of natural catalase was produced, isolated and biochemically characterized. The recombinant protein showed IgE reactivity in 7 out of 13 tested patients with suspected allergy to banana in immunoblot. Novel banana fruit allergens should be added as components to allergen-microarrays for the diagnosis and the monitoring of banana allergy. Significance: By employment of CPLL methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis catalase from banana fruit is identified as a novel allergen, with proposed designation as Mus a 7. IgE reactive recombinant Mus a 7 was produced and should be included in a component-resolved allergy diagnosis.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Proteomics",
title = "Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana",
volume = "175",
pages = "87-94",
doi = "10.1016/j.jprot.2018.01.007"
}

Nikolić, J., Nešić, A. N., Kull, S., Schocker, F., Jappe, U.,& Gavrović-Jankulović, M.. (2018). Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana. in Journal of Proteomics
Elsevier Science Bv, Amsterdam., 175, 87-94.
https://doi.org/10.1016/j.jprot.2018.01.007

Nikolić J, Nešić AN, Kull S, Schocker F, Jappe U, Gavrović-Jankulović M. Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana. in Journal of Proteomics. 2018;175:87-94.
doi:10.1016/j.jprot.2018.01.007 .

Nikolić, Jasna, Nešić, Andrijana N., Kull, Skadi, Schocker, Frauke, Jappe, Uta, Gavrović-Jankulović, Marija, "Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana" in Journal of Proteomics, 175 (2018):87-94,
https://doi.org/10.1016/j.jprot.2018.01.007 . .

TY  - DATA
AU  - Nikolić, Jasna
AU  - Nešić, Andrijana N.
AU  - Kull, Skadi
AU  - Schocker, Frauke
AU  - Jappe, Uta
AU  - Gavrović-Jankulović, Marija
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3203
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Proteomics
T1  - Supplementary material for the article: Nikolić, J.; Nešić, A.; Kull, S.; Schocker, F.; Jappe, U.; Gavrović-Jankulović, M.  Employment of Proteomic and Immunological Based Methods for the Identification of  Catalase as Novel Allergen from Banana. Journal of Proteomics 2018, 175, 87–94.  https://doi.org/10.1016/j.jprot.2018.01.007
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3203
ER  - 

@misc{
author = "Nikolić, Jasna and Nešić, Andrijana N. and Kull, Skadi and Schocker, Frauke and Jappe, Uta and Gavrović-Jankulović, Marija",
year = "2018",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Proteomics",
title = "Supplementary material for the article: Nikolić, J.; Nešić, A.; Kull, S.; Schocker, F.; Jappe, U.; Gavrović-Jankulović, M.  Employment of Proteomic and Immunological Based Methods for the Identification of  Catalase as Novel Allergen from Banana. Journal of Proteomics 2018, 175, 87–94.  https://doi.org/10.1016/j.jprot.2018.01.007",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3203"
}

Nikolić, J., Nešić, A. N., Kull, S., Schocker, F., Jappe, U.,& Gavrović-Jankulović, M.. (2018). Supplementary material for the article: Nikolić, J.; Nešić, A.; Kull, S.; Schocker, F.; Jappe, U.; Gavrović-Jankulović, M.  Employment of Proteomic and Immunological Based Methods for the Identification of  Catalase as Novel Allergen from Banana. Journal of Proteomics 2018, 175, 87–94.  https://doi.org/10.1016/j.jprot.2018.01.007. in Journal of Proteomics
Elsevier Science Bv, Amsterdam..
https://hdl.handle.net/21.15107/rcub_cherry_3203

Nikolić J, Nešić AN, Kull S, Schocker F, Jappe U, Gavrović-Jankulović M. Supplementary material for the article: Nikolić, J.; Nešić, A.; Kull, S.; Schocker, F.; Jappe, U.; Gavrović-Jankulović, M.  Employment of Proteomic and Immunological Based Methods for the Identification of  Catalase as Novel Allergen from Banana. Journal of Proteomics 2018, 175, 87–94.  https://doi.org/10.1016/j.jprot.2018.01.007. in Journal of Proteomics. 2018;.
https://hdl.handle.net/21.15107/rcub_cherry_3203 .

Nikolić, Jasna, Nešić, Andrijana N., Kull, Skadi, Schocker, Frauke, Jappe, Uta, Gavrović-Jankulović, Marija, "Supplementary material for the article: Nikolić, J.; Nešić, A.; Kull, S.; Schocker, F.; Jappe, U.; Gavrović-Jankulović, M.  Employment of Proteomic and Immunological Based Methods for the Identification of  Catalase as Novel Allergen from Banana. Journal of Proteomics 2018, 175, 87–94.  https://doi.org/10.1016/j.jprot.2018.01.007" in Journal of Proteomics (2018),
https://hdl.handle.net/21.15107/rcub_cherry_3203 .

TY  - JOUR
AU  - Nikolić, Jasna
AU  - Nešić, Andrijana N.
AU  - Čavić, Milena
AU  - Đorđević, Neda O.
AU  - Anđelković, Uroš
AU  - Atanasković-Marković, Marina
AU  - Drakulić, Branko J.
AU  - Gavrović-Jankulović, Marija
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2384
AB  - Background: Protein oxidation can occur as a consequence of lipid peroxidation during food processing. The aim of this work was to explore the effect of malondialdehyde (MDA) modification of ovalbumin (OVA) on its interaction with T84 intestinal cells. Methods: Molecular dynamics simulation was employed for the prediction of MDA modification in the OVA, while introduced structural changes were evaluated by measurement of carbonyl group content, fluorescence spectra, MS/MS analysis, and IgE reactivity. Effects of MDA modified OVA on T84 epithelial cells were analyzed by gene expression for pro-inflammatory cytokines and protein secretion. Results: Out of 9 predicted, five modified Lys residues were confirmed by MS/MS analysis: (51)TQINKVVR(58), (85)DILNQITKPNDVYSFSLASR(104), (111)YPILPEYLQCVKELYR(126), (187)AFKDEDTQAMPFR(99), (KIKVYLPR284)-K-277, and (IKVYLPR284)-I-278. The introduced MDA modifications influenced profile of IgE reactivity to OVA. Treatment of T84 epithelial cells with OVA and OVA modified with 1 mM MDA, induced up-regulation of pro-inflammatory cytokines (IL-1 beta,IL-25, IL-33, TSLP and TNF alpha), while OVA modification with 10 mM MDA induced down regulation of the cytokine expression profile, except for IL-1 beta. OVA and OVA modified with 1 mM MDA induced secretion of epithelial cells specific cytokine IL-33. Conclusions: This finding indicated that OVA and its MDA modified form have the potential to trigger the innate immunity by inducing up-regulation and secretion of pro-allergenic IL-33 in T84 intestinal epithelial cells. General significance: Interactions of ovalbumin and its MDA modified form with intestinal epithelial cells can induce a specific immunological priming necessary for the downstream activation of innate immunity. (C) 2016 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Biochimica et Biophysica Acta: General Subjects
T1  - Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells
VL  - 1861
IS  - 2
SP  - 126
EP  - 134
DO  - 10.1016/j.bbagen.2016.11.021
ER  - 

@article{
author = "Nikolić, Jasna and Nešić, Andrijana N. and Čavić, Milena and Đorđević, Neda O. and Anđelković, Uroš and Atanasković-Marković, Marina and Drakulić, Branko J. and Gavrović-Jankulović, Marija",
year = "2017",
abstract = "Background: Protein oxidation can occur as a consequence of lipid peroxidation during food processing. The aim of this work was to explore the effect of malondialdehyde (MDA) modification of ovalbumin (OVA) on its interaction with T84 intestinal cells. Methods: Molecular dynamics simulation was employed for the prediction of MDA modification in the OVA, while introduced structural changes were evaluated by measurement of carbonyl group content, fluorescence spectra, MS/MS analysis, and IgE reactivity. Effects of MDA modified OVA on T84 epithelial cells were analyzed by gene expression for pro-inflammatory cytokines and protein secretion. Results: Out of 9 predicted, five modified Lys residues were confirmed by MS/MS analysis: (51)TQINKVVR(58), (85)DILNQITKPNDVYSFSLASR(104), (111)YPILPEYLQCVKELYR(126), (187)AFKDEDTQAMPFR(99), (KIKVYLPR284)-K-277, and (IKVYLPR284)-I-278. The introduced MDA modifications influenced profile of IgE reactivity to OVA. Treatment of T84 epithelial cells with OVA and OVA modified with 1 mM MDA, induced up-regulation of pro-inflammatory cytokines (IL-1 beta,IL-25, IL-33, TSLP and TNF alpha), while OVA modification with 10 mM MDA induced down regulation of the cytokine expression profile, except for IL-1 beta. OVA and OVA modified with 1 mM MDA induced secretion of epithelial cells specific cytokine IL-33. Conclusions: This finding indicated that OVA and its MDA modified form have the potential to trigger the innate immunity by inducing up-regulation and secretion of pro-allergenic IL-33 in T84 intestinal epithelial cells. General significance: Interactions of ovalbumin and its MDA modified form with intestinal epithelial cells can induce a specific immunological priming necessary for the downstream activation of innate immunity. (C) 2016 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Biochimica et Biophysica Acta: General Subjects",
title = "Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells",
volume = "1861",
number = "2",
pages = "126-134",
doi = "10.1016/j.bbagen.2016.11.021"
}

Nikolić, J., Nešić, A. N., Čavić, M., Đorđević, N. O., Anđelković, U., Atanasković-Marković, M., Drakulić, B. J.,& Gavrović-Jankulović, M.. (2017). Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells. in Biochimica et Biophysica Acta: General Subjects
Elsevier Science Bv, Amsterdam., 1861(2), 126-134.
https://doi.org/10.1016/j.bbagen.2016.11.021

Nikolić J, Nešić AN, Čavić M, Đorđević NO, Anđelković U, Atanasković-Marković M, Drakulić BJ, Gavrović-Jankulović M. Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells. in Biochimica et Biophysica Acta: General Subjects. 2017;1861(2):126-134.
doi:10.1016/j.bbagen.2016.11.021 .

Nikolić, Jasna, Nešić, Andrijana N., Čavić, Milena, Đorđević, Neda O., Anđelković, Uroš, Atanasković-Marković, Marina, Drakulić, Branko J., Gavrović-Jankulović, Marija, "Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells" in Biochimica et Biophysica Acta: General Subjects, 1861, no. 2 (2017):126-134,
https://doi.org/10.1016/j.bbagen.2016.11.021 . .

TY  - JOUR
AU  - Nikolić, Jasna
AU  - Nešić, Andrijana N.
AU  - Čavić, Milena
AU  - Đorđević, Neda O.
AU  - Anđelković, Uroš
AU  - Atanasković-Marković, Marina
AU  - Drakulić, Branko J.
AU  - Gavrović-Jankulović, Marija
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2384
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2954
AB  - ackground: Protein oxidation can occur as a consequence of lipid peroxidation during food processing. The aim of this work was to explore the effect of malondialdehyde (MDA) modification of ovalbumin (OVA) on its interaction with T84 intestinal cells. Methods: Molecular dynamics simulation was employed for the prediction of MDA modification in the OVA, while introduced structural changes were evaluated by measurement of carbonyl group content, fluorescence spectra, MS/MS analysis, and IgE reactivity. Effects of MDA modified OVA on T84 epithelial cells were analyzed by gene expression for pro-inflammatory cytokines and protein secretion. Results: Out of 9 predicted, five modified Lys residues were confirmed by MS/MS analysis: (51)TQINKVVR(58), (85)DILNQITKPNDVYSFSLASR(104), (111)YPILPEYLQCVKELYR(126), (187)AFKDEDTQAMPFR(99), (KIKVYLPR284)-K-277, and (IKVYLPR284)-I-278. The introduced MDA modifications influenced profile of IgE reactivity to OVA. Treatment of T84 epithelial cells with OVA and OVA modified with 1 mM MDA, induced up-regulation of pro-inflammatory cytokines (IL-1 beta,IL-25, IL-33, TSLP and TNF alpha), while OVA modification with 10 mM MDA induced down regulation of the cytokine expression profile, except for IL-1 beta. OVA and OVA modified with 1 mM MDA induced secretion of epithelial cells specific cytokine IL-33. Conclusions: This finding indicated that OVA and its MDA modified form have the potential to trigger the innate immunity by inducing up-regulation and secretion of pro-allergenic IL-33 in T84 intestinal epithelial cells. General significance: Interactions of ovalbumin and its MDA modified form with intestinal epithelial cells can induce a specific immunological priming necessary for the downstream activation of innate immunity. (C) 2016 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Biochimica et Biophysica Acta: General Subjects
T1  - Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells
VL  - 1861
IS  - 2
SP  - 126
EP  - 134
DO  - 10.1016/j.bbagen.2016.11.021
ER  - 

@article{
author = "Nikolić, Jasna and Nešić, Andrijana N. and Čavić, Milena and Đorđević, Neda O. and Anđelković, Uroš and Atanasković-Marković, Marina and Drakulić, Branko J. and Gavrović-Jankulović, Marija",
year = "2017",
abstract = "ackground: Protein oxidation can occur as a consequence of lipid peroxidation during food processing. The aim of this work was to explore the effect of malondialdehyde (MDA) modification of ovalbumin (OVA) on its interaction with T84 intestinal cells. Methods: Molecular dynamics simulation was employed for the prediction of MDA modification in the OVA, while introduced structural changes were evaluated by measurement of carbonyl group content, fluorescence spectra, MS/MS analysis, and IgE reactivity. Effects of MDA modified OVA on T84 epithelial cells were analyzed by gene expression for pro-inflammatory cytokines and protein secretion. Results: Out of 9 predicted, five modified Lys residues were confirmed by MS/MS analysis: (51)TQINKVVR(58), (85)DILNQITKPNDVYSFSLASR(104), (111)YPILPEYLQCVKELYR(126), (187)AFKDEDTQAMPFR(99), (KIKVYLPR284)-K-277, and (IKVYLPR284)-I-278. The introduced MDA modifications influenced profile of IgE reactivity to OVA. Treatment of T84 epithelial cells with OVA and OVA modified with 1 mM MDA, induced up-regulation of pro-inflammatory cytokines (IL-1 beta,IL-25, IL-33, TSLP and TNF alpha), while OVA modification with 10 mM MDA induced down regulation of the cytokine expression profile, except for IL-1 beta. OVA and OVA modified with 1 mM MDA induced secretion of epithelial cells specific cytokine IL-33. Conclusions: This finding indicated that OVA and its MDA modified form have the potential to trigger the innate immunity by inducing up-regulation and secretion of pro-allergenic IL-33 in T84 intestinal epithelial cells. General significance: Interactions of ovalbumin and its MDA modified form with intestinal epithelial cells can induce a specific immunological priming necessary for the downstream activation of innate immunity. (C) 2016 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Biochimica et Biophysica Acta: General Subjects",
title = "Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells",
volume = "1861",
number = "2",
pages = "126-134",
doi = "10.1016/j.bbagen.2016.11.021"
}

Nikolić, J., Nešić, A. N., Čavić, M., Đorđević, N. O., Anđelković, U., Atanasković-Marković, M., Drakulić, B. J.,& Gavrović-Jankulović, M.. (2017). Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells. in Biochimica et Biophysica Acta: General Subjects
Elsevier Science Bv, Amsterdam., 1861(2), 126-134.
https://doi.org/10.1016/j.bbagen.2016.11.021

Nikolić J, Nešić AN, Čavić M, Đorđević NO, Anđelković U, Atanasković-Marković M, Drakulić BJ, Gavrović-Jankulović M. Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells. in Biochimica et Biophysica Acta: General Subjects. 2017;1861(2):126-134.
doi:10.1016/j.bbagen.2016.11.021 .

Nikolić, Jasna, Nešić, Andrijana N., Čavić, Milena, Đorđević, Neda O., Anđelković, Uroš, Atanasković-Marković, Marina, Drakulić, Branko J., Gavrović-Jankulović, Marija, "Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells" in Biochimica et Biophysica Acta: General Subjects, 1861, no. 2 (2017):126-134,
https://doi.org/10.1016/j.bbagen.2016.11.021 . .

TY  - THES
AU  - Nikolić, Jasna
PY  - 2017
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=5576
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:17052/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=49837839
UR  - http://nardus.mpn.gov.rs/123456789/9168
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2739
AB  - Banana je voće koje se veoma često koristi u ishrani širom sveta. Predstavljajednu od prvih čvrstih namirnica koje se uvode u ishranu kod novorođenčadi, kao ijednu od uobičajnih namirnica u ishrani hospitalizovanih pacijenata. Prijatnog je ukusa ilake svarljivosti, i bogata je nutrijentima. Ipak, pored svih dobrih strana korišćenjabanane u ishrani, ona može biti uzročnik netolerancije i alergijske reakcije.Alergijska reakcija na bananu je u većini slučajeva praćena pojavom blagihlokalnih simptoma, međutim u retkim slučajevima dolazi i do anafilaktičke reakcije.Dijagnostički reagensi koji se rutinski primenjuju u kliničkoj dijagnostici zaalergiju na bananu su se u praksi pokazali kao nepouzdani. Kao pokazatelj važnostiupotrebe pouzdanih dijagnostičkih reagenasa opisani su slučajevi anafilaktičke reakcijenakon konzumiranja banane, gde je pokazano da komercijalno dostupni reagensi nisudovoljno osetljivi da ukažu na prisustvo specifičnih IgE antitela, odnosno da postojirazvijena alergija na bananu. U cilju unapređenja dijagostičkih reagenasa za alergiju nabananu u ovom radu je optimizovan postupak za prečišćavanje tri važna alergena izbanana, označena kao Mus a 2, Mus a 4 i Mus a 5. Dobijeni pojedinačni dijagnostičkireagensi za alergiju na bananu su omogućili detekciju specifičnog IgE-a kod alergičnihosoba i pokazali su se pouzdanijim u odnosu na komercijanli alergenski ekstrakt.U literaturi, kao i u rezultatima naših istraživanja, je pokazano da osim do sadaregistrovanih alergena iz banane (WHO/IUIS baza podataka) postoji još IgE reaktivnihproteina banane koji bi mogli da se koriste radi unapređenja dijagnostifikovanja alergijena ovaj alergeni izvor. Upotrebom kombinatornih biblioteka peptidnih liganada (CPLL)razvijen je 2D imunoblot proteina iz ekstrakta banane. Detektovani su IgE reaktivniproteini banane, među kojima su i neki koji do sada nisu registrovani kao alergeni.Identifikacija proteina sa 2D mape je urađena tehnikom određivanja “proteinskog otiskaprsta" (PMF). Među IgE reaktivnim proteinima banane identifikovana je i katalaza, kojaje proizvedena rekombinantnom DNK tehnologijom i okarakterisana kao novi alergenbanane...
PB  - Универзитет у Београду, Хемијски факултет
T2  - Универзитет у Београду
T1  - Unapređenje dijagnostičkih reagenasa za alergiju na bananu
UR  - https://hdl.handle.net/21.15107/rcub_nardus_9168
ER  - 

@phdthesis{
author = "Nikolić, Jasna",
year = "2017",
abstract = "Banana je voće koje se veoma često koristi u ishrani širom sveta. Predstavljajednu od prvih čvrstih namirnica koje se uvode u ishranu kod novorođenčadi, kao ijednu od uobičajnih namirnica u ishrani hospitalizovanih pacijenata. Prijatnog je ukusa ilake svarljivosti, i bogata je nutrijentima. Ipak, pored svih dobrih strana korišćenjabanane u ishrani, ona može biti uzročnik netolerancije i alergijske reakcije.Alergijska reakcija na bananu je u većini slučajeva praćena pojavom blagihlokalnih simptoma, međutim u retkim slučajevima dolazi i do anafilaktičke reakcije.Dijagnostički reagensi koji se rutinski primenjuju u kliničkoj dijagnostici zaalergiju na bananu su se u praksi pokazali kao nepouzdani. Kao pokazatelj važnostiupotrebe pouzdanih dijagnostičkih reagenasa opisani su slučajevi anafilaktičke reakcijenakon konzumiranja banane, gde je pokazano da komercijalno dostupni reagensi nisudovoljno osetljivi da ukažu na prisustvo specifičnih IgE antitela, odnosno da postojirazvijena alergija na bananu. U cilju unapređenja dijagostičkih reagenasa za alergiju nabananu u ovom radu je optimizovan postupak za prečišćavanje tri važna alergena izbanana, označena kao Mus a 2, Mus a 4 i Mus a 5. Dobijeni pojedinačni dijagnostičkireagensi za alergiju na bananu su omogućili detekciju specifičnog IgE-a kod alergičnihosoba i pokazali su se pouzdanijim u odnosu na komercijanli alergenski ekstrakt.U literaturi, kao i u rezultatima naših istraživanja, je pokazano da osim do sadaregistrovanih alergena iz banane (WHO/IUIS baza podataka) postoji još IgE reaktivnihproteina banane koji bi mogli da se koriste radi unapređenja dijagnostifikovanja alergijena ovaj alergeni izvor. Upotrebom kombinatornih biblioteka peptidnih liganada (CPLL)razvijen je 2D imunoblot proteina iz ekstrakta banane. Detektovani su IgE reaktivniproteini banane, među kojima su i neki koji do sada nisu registrovani kao alergeni.Identifikacija proteina sa 2D mape je urađena tehnikom određivanja “proteinskog otiskaprsta" (PMF). Među IgE reaktivnim proteinima banane identifikovana je i katalaza, kojaje proizvedena rekombinantnom DNK tehnologijom i okarakterisana kao novi alergenbanane...",
publisher = "Универзитет у Београду, Хемијски факултет",
journal = "Универзитет у Београду",
title = "Unapređenje dijagnostičkih reagenasa za alergiju na bananu",
url = "https://hdl.handle.net/21.15107/rcub_nardus_9168"
}

Nikolić, J.. (2017). Unapređenje dijagnostičkih reagenasa za alergiju na bananu. in Универзитет у Београду
Универзитет у Београду, Хемијски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_9168

Nikolić J. Unapređenje dijagnostičkih reagenasa za alergiju na bananu. in Универзитет у Београду. 2017;.
https://hdl.handle.net/21.15107/rcub_nardus_9168 .

Nikolić, Jasna, "Unapređenje dijagnostičkih reagenasa za alergiju na bananu" in Универзитет у Београду (2017),
https://hdl.handle.net/21.15107/rcub_nardus_9168 .

TY  - CHAP
AU  - Nikolić, Jasna
AU  - Popović, Milica M.
AU  - Gavrović-Jankulović, Marija
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/314
AB  - Banana is a perennial herbaceous plant widely cultivated in the tropical and subtropical regions. The pulp of the fruit is a rich source of minerals, vitamins, antioxidants, low glycemic carbohydrates, and fiber, and thereby its consumption has beneficial effects on human health. These nutritional values and its pleasant taste induced the introduction of banana fruit into human diet in infancy and also during convalescence. However, in spite of positive health effects, banana fruit has been recognized as an important food allergen source. The clinical manifestations of banana allergy have usually been associated with mild, local symptoms denoted as oral allergy syndrome. However, more severe reactions, as well as cases of anaphylactic reactions to banana fruit have been registered. IgE reactivity of banana is associated with different proteins, and, so far, only six allergens have been identified and characterized: profilin - actin binding protein (Mus a 1), a class 1 chitinase (Mus a 2), non-specific lipid transfer protein (Mus a 3), thaumatin-like protein (Mus a 4), beta-1,3-glucanase (Mus a 5), and recently registered ascorbate peroxidase (Mus a 6). In this review, we will address the structural features of identified banana allergens and correlate in vitro and in vivo clinical reactivity with their structural homologs from other allergen sources. © 2016 Nova Science Publishers, Inc.
T2  - Bananas: Cultivation, Consumption and Crop Diseases
T1  - Banana as a food allergen source
SP  - 107
EP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_cherry_314
ER  - 

@inbook{
author = "Nikolić, Jasna and Popović, Milica M. and Gavrović-Jankulović, Marija",
year = "2016",
abstract = "Banana is a perennial herbaceous plant widely cultivated in the tropical and subtropical regions. The pulp of the fruit is a rich source of minerals, vitamins, antioxidants, low glycemic carbohydrates, and fiber, and thereby its consumption has beneficial effects on human health. These nutritional values and its pleasant taste induced the introduction of banana fruit into human diet in infancy and also during convalescence. However, in spite of positive health effects, banana fruit has been recognized as an important food allergen source. The clinical manifestations of banana allergy have usually been associated with mild, local symptoms denoted as oral allergy syndrome. However, more severe reactions, as well as cases of anaphylactic reactions to banana fruit have been registered. IgE reactivity of banana is associated with different proteins, and, so far, only six allergens have been identified and characterized: profilin - actin binding protein (Mus a 1), a class 1 chitinase (Mus a 2), non-specific lipid transfer protein (Mus a 3), thaumatin-like protein (Mus a 4), beta-1,3-glucanase (Mus a 5), and recently registered ascorbate peroxidase (Mus a 6). In this review, we will address the structural features of identified banana allergens and correlate in vitro and in vivo clinical reactivity with their structural homologs from other allergen sources. © 2016 Nova Science Publishers, Inc.",
journal = "Bananas: Cultivation, Consumption and Crop Diseases",
booktitle = "Banana as a food allergen source",
pages = "107-126",
url = "https://hdl.handle.net/21.15107/rcub_cherry_314"
}

Nikolić, J., Popović, M. M.,& Gavrović-Jankulović, M.. (2016). Banana as a food allergen source. in Bananas: Cultivation, Consumption and Crop Diseases, 107-126.
https://hdl.handle.net/21.15107/rcub_cherry_314

Nikolić J, Popović MM, Gavrović-Jankulović M. Banana as a food allergen source. in Bananas: Cultivation, Consumption and Crop Diseases. 2016;:107-126.
https://hdl.handle.net/21.15107/rcub_cherry_314 .

Nikolić, Jasna, Popović, Milica M., Gavrović-Jankulović, Marija, "Banana as a food allergen source" in Bananas: Cultivation, Consumption and Crop Diseases (2016):107-126,
https://hdl.handle.net/21.15107/rcub_cherry_314 .

TY  - JOUR
AU  - Jappe, Uta
AU  - Nikolić, Jasna
AU  - Opitz, Annika
AU  - Homann, Arne
AU  - Zabel, Peter
AU  - Gavrović-Jankulović, Marija
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2276
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of the European Academy of Dermatology and Venereology
T1  - Apparent IgE negative anaphylactic reaction to banana combined with kiwi allergy - complementary diagnostic value of purified single banana allergens
VL  - 30
IS  - 7
SP  - 1220
EP  - 1222
DO  - 10.1111/jdv.13146
ER  - 

@article{
author = "Jappe, Uta and Nikolić, Jasna and Opitz, Annika and Homann, Arne and Zabel, Peter and Gavrović-Jankulović, Marija",
year = "2016",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of the European Academy of Dermatology and Venereology",
title = "Apparent IgE negative anaphylactic reaction to banana combined with kiwi allergy - complementary diagnostic value of purified single banana allergens",
volume = "30",
number = "7",
pages = "1220-1222",
doi = "10.1111/jdv.13146"
}

Jappe, U., Nikolić, J., Opitz, A., Homann, A., Zabel, P.,& Gavrović-Jankulović, M.. (2016). Apparent IgE negative anaphylactic reaction to banana combined with kiwi allergy - complementary diagnostic value of purified single banana allergens. in Journal of the European Academy of Dermatology and Venereology
Wiley-Blackwell, Hoboken., 30(7), 1220-1222.
https://doi.org/10.1111/jdv.13146

Jappe U, Nikolić J, Opitz A, Homann A, Zabel P, Gavrović-Jankulović M. Apparent IgE negative anaphylactic reaction to banana combined with kiwi allergy - complementary diagnostic value of purified single banana allergens. in Journal of the European Academy of Dermatology and Venereology. 2016;30(7):1220-1222.
doi:10.1111/jdv.13146 .

Jappe, Uta, Nikolić, Jasna, Opitz, Annika, Homann, Arne, Zabel, Peter, Gavrović-Jankulović, Marija, "Apparent IgE negative anaphylactic reaction to banana combined with kiwi allergy - complementary diagnostic value of purified single banana allergens" in Journal of the European Academy of Dermatology and Venereology, 30, no. 7 (2016):1220-1222,
https://doi.org/10.1111/jdv.13146 . .

TY  - JOUR
AU  - Bonnin, Estelle
AU  - Mutić, Jelena
AU  - Nikolić, Jasna
AU  - Burr, Sally
AU  - Robert, Paul
AU  - Crepeau, Marie-Jeanne
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1697
AB  - Pectin gels and pectin-cellulose binary gels were used as cell wall-mimicking systems to investigate the diffusion ability of a fungal pectin methylesterase. Increasing content of cellulose in the gel appears to result: (i) in longer demethylated blocks thus favouring AaPME processivity, and (ii) in accelerated enzyme kinetics. To better understand this unexpected behaviour, a method was set up to investigate the gel porosity as a function of the cellulose content by following the passive diffusion of three pullulans having different hydrodynamic volumes. Like the enzyme, the pullulans diffused more efficiently in the gels containing the highest proportions of cellulose. Altogether, these results suggest that the gel settled differently during formation according to the respective proportions of the two polysaccharides. With cellulose present, a fraction of pectin would form close interactions with the microfibrils resulting in a larger volume accessible to diffusing molecules. This volume would be related to the cellulose concentration.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Methylesterase behaviour is related to polysaccharide organisation in model systems mimicking cell walls
VL  - 124
SP  - 57
EP  - 65
DO  - 10.1016/j.carbpol.2015.01.074
ER  - 

@article{
author = "Bonnin, Estelle and Mutić, Jelena and Nikolić, Jasna and Burr, Sally and Robert, Paul and Crepeau, Marie-Jeanne",
year = "2015",
abstract = "Pectin gels and pectin-cellulose binary gels were used as cell wall-mimicking systems to investigate the diffusion ability of a fungal pectin methylesterase. Increasing content of cellulose in the gel appears to result: (i) in longer demethylated blocks thus favouring AaPME processivity, and (ii) in accelerated enzyme kinetics. To better understand this unexpected behaviour, a method was set up to investigate the gel porosity as a function of the cellulose content by following the passive diffusion of three pullulans having different hydrodynamic volumes. Like the enzyme, the pullulans diffused more efficiently in the gels containing the highest proportions of cellulose. Altogether, these results suggest that the gel settled differently during formation according to the respective proportions of the two polysaccharides. With cellulose present, a fraction of pectin would form close interactions with the microfibrils resulting in a larger volume accessible to diffusing molecules. This volume would be related to the cellulose concentration.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Methylesterase behaviour is related to polysaccharide organisation in model systems mimicking cell walls",
volume = "124",
pages = "57-65",
doi = "10.1016/j.carbpol.2015.01.074"
}

Bonnin, E., Mutić, J., Nikolić, J., Burr, S., Robert, P.,& Crepeau, M.. (2015). Methylesterase behaviour is related to polysaccharide organisation in model systems mimicking cell walls. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 124, 57-65.
https://doi.org/10.1016/j.carbpol.2015.01.074

Bonnin E, Mutić J, Nikolić J, Burr S, Robert P, Crepeau M. Methylesterase behaviour is related to polysaccharide organisation in model systems mimicking cell walls. in Carbohydrate Polymers. 2015;124:57-65.
doi:10.1016/j.carbpol.2015.01.074 .

Bonnin, Estelle, Mutić, Jelena, Nikolić, Jasna, Burr, Sally, Robert, Paul, Crepeau, Marie-Jeanne, "Methylesterase behaviour is related to polysaccharide organisation in model systems mimicking cell walls" in Carbohydrate Polymers, 124 (2015):57-65,
https://doi.org/10.1016/j.carbpol.2015.01.074 . .

TY  - JOUR
AU  - Nikolić, Jasna
AU  - Mrkić, Ivan
AU  - Grozdanović, Milica
AU  - Popović, Milica M.
AU  - Petersen, Arnd
AU  - Jappe, Uta
AU  - Gavrović-Jankulović, Marija
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1804
AB  - Banana fruit (Musa acuminata) has become an important food allergen source in recent years. So far, 5 IgE reactive banana proteins have been identified, and the major allergens are: Mus a 2 (a class I chitinase, 31 kDa),Mus a4 (thaumatin-like protein, 21 kDa), and Mus a 5 (beta-1,3-glucanase,33 kDa). Due to variations in allergen expression levels, diagnostic reagents for food allergy can be improved by using individual allergen components instead of banana allergen extracts. The purpose of this study was to optimize the purification protocol of the three major allergens present in banana fruit: Mus a 2, Mus a 4 and Mus a 5. By employing a three-step purification protocol (a combination of anion-exchange, cation-exchange and reversed-phase chromatography) three important banana allergens were obtained in sufficient yield and high purity. Characterization of the purified proteins was performed by both biochemical (2-D PAGE, mass fingerprint and N-terminal sequencing) and immunochemical (immunoblot) methods. IgE reactivity to the purified allergens was tested by employing sera of five allergic patients. The purified allergens displayed higher sensitivity in IgE detection than the routinely used extracts. The three purified allergens are good candidates for reagents in component-based diagnosis of banana allergy. (C) 2014 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Chromatography B: Analytical Technologies in the Biomedical and L
T1  - Protocol for simultaneous isolation of three important banana allergens
VL  - 962
SP  - 30
EP  - 36
DO  - 10.1016/j.jchromb.2014.05.020
ER  - 

@article{
author = "Nikolić, Jasna and Mrkić, Ivan and Grozdanović, Milica and Popović, Milica M. and Petersen, Arnd and Jappe, Uta and Gavrović-Jankulović, Marija",
year = "2014",
abstract = "Banana fruit (Musa acuminata) has become an important food allergen source in recent years. So far, 5 IgE reactive banana proteins have been identified, and the major allergens are: Mus a 2 (a class I chitinase, 31 kDa),Mus a4 (thaumatin-like protein, 21 kDa), and Mus a 5 (beta-1,3-glucanase,33 kDa). Due to variations in allergen expression levels, diagnostic reagents for food allergy can be improved by using individual allergen components instead of banana allergen extracts. The purpose of this study was to optimize the purification protocol of the three major allergens present in banana fruit: Mus a 2, Mus a 4 and Mus a 5. By employing a three-step purification protocol (a combination of anion-exchange, cation-exchange and reversed-phase chromatography) three important banana allergens were obtained in sufficient yield and high purity. Characterization of the purified proteins was performed by both biochemical (2-D PAGE, mass fingerprint and N-terminal sequencing) and immunochemical (immunoblot) methods. IgE reactivity to the purified allergens was tested by employing sera of five allergic patients. The purified allergens displayed higher sensitivity in IgE detection than the routinely used extracts. The three purified allergens are good candidates for reagents in component-based diagnosis of banana allergy. (C) 2014 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and L",
title = "Protocol for simultaneous isolation of three important banana allergens",
volume = "962",
pages = "30-36",
doi = "10.1016/j.jchromb.2014.05.020"
}

Nikolić, J., Mrkić, I., Grozdanović, M., Popović, M. M., Petersen, A., Jappe, U.,& Gavrović-Jankulović, M.. (2014). Protocol for simultaneous isolation of three important banana allergens. in Journal of Chromatography B: Analytical Technologies in the Biomedical and L
Elsevier Science Bv, Amsterdam., 962, 30-36.
https://doi.org/10.1016/j.jchromb.2014.05.020

Nikolić J, Mrkić I, Grozdanović M, Popović MM, Petersen A, Jappe U, Gavrović-Jankulović M. Protocol for simultaneous isolation of three important banana allergens. in Journal of Chromatography B: Analytical Technologies in the Biomedical and L. 2014;962:30-36.
doi:10.1016/j.jchromb.2014.05.020 .

Nikolić, Jasna, Mrkić, Ivan, Grozdanović, Milica, Popović, Milica M., Petersen, Arnd, Jappe, Uta, Gavrović-Jankulović, Marija, "Protocol for simultaneous isolation of three important banana allergens" in Journal of Chromatography B: Analytical Technologies in the Biomedical and L, 962 (2014):30-36,
https://doi.org/10.1016/j.jchromb.2014.05.020 . .

TY  - JOUR
AU  - Mrkić, Ivan
AU  - Abughren, Mohamed
AU  - Nikolić, Jasna
AU  - Anđelković, Uroš
AU  - Vassilopoulou, Emilia
AU  - Sinaniotis, Athanassios
AU  - Petersen, Arnd
AU  - Papadopoulos, Nikolaos G.
AU  - Gavrović-Jankulović, Marija
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1774
AB  - Allergy to banana fruit appears to have become an important cause of fruit allergy in Europe. Among five allergens that have been found, beta-1,3-glucanase denoted as Mus a 5 was identified as a candidate allergen for the component-resolved allergy diagnosis of banana allergy. Because of the variations in protein levels in banana fruit, in this study Mus a 5 was produced as a fusion protein with glutathione-S-transferase in Escherichia coli. The recombinant Mus a 5 was purified under native conditions by a combination of affinity, ion-exchange, and reversed phase chromatography. N-terminal sequence was confirmed by Edman degradation and 55 % of the primary structure was identified by mass fingerprint, while the secondary structure was assessed by circular dichroism spectroscopy. IgG reactivity of recombinant protein was shown in 2-D immunoblot with anti-Mus a 5 antibodies, while IgG and IgE binding to natural Mus a 5 was inhibited with the recombinant Mus a 5 in immunoblot inhibition test. IgE reactivity of recombinant Mus a 5 was shown in ELISA within a group of ten persons sensitized to banana fruit. Recombinant Mus a 5 is a novel reagent suitable for the component-resolved allergy diagnosis of banana allergy.
PB  - Humana Press Inc, Totowa
T2  - Molecular Biotechnology
T1  - Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit
VL  - 56
IS  - 6
SP  - 498
EP  - 506
DO  - 10.1007/s12033-013-9719-8
ER  - 

@article{
author = "Mrkić, Ivan and Abughren, Mohamed and Nikolić, Jasna and Anđelković, Uroš and Vassilopoulou, Emilia and Sinaniotis, Athanassios and Petersen, Arnd and Papadopoulos, Nikolaos G. and Gavrović-Jankulović, Marija",
year = "2014",
abstract = "Allergy to banana fruit appears to have become an important cause of fruit allergy in Europe. Among five allergens that have been found, beta-1,3-glucanase denoted as Mus a 5 was identified as a candidate allergen for the component-resolved allergy diagnosis of banana allergy. Because of the variations in protein levels in banana fruit, in this study Mus a 5 was produced as a fusion protein with glutathione-S-transferase in Escherichia coli. The recombinant Mus a 5 was purified under native conditions by a combination of affinity, ion-exchange, and reversed phase chromatography. N-terminal sequence was confirmed by Edman degradation and 55 % of the primary structure was identified by mass fingerprint, while the secondary structure was assessed by circular dichroism spectroscopy. IgG reactivity of recombinant protein was shown in 2-D immunoblot with anti-Mus a 5 antibodies, while IgG and IgE binding to natural Mus a 5 was inhibited with the recombinant Mus a 5 in immunoblot inhibition test. IgE reactivity of recombinant Mus a 5 was shown in ELISA within a group of ten persons sensitized to banana fruit. Recombinant Mus a 5 is a novel reagent suitable for the component-resolved allergy diagnosis of banana allergy.",
publisher = "Humana Press Inc, Totowa",
journal = "Molecular Biotechnology",
title = "Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit",
volume = "56",
number = "6",
pages = "498-506",
doi = "10.1007/s12033-013-9719-8"
}

Mrkić, I., Abughren, M., Nikolić, J., Anđelković, U., Vassilopoulou, E., Sinaniotis, A., Petersen, A., Papadopoulos, N. G.,& Gavrović-Jankulović, M.. (2014). Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit. in Molecular Biotechnology
Humana Press Inc, Totowa., 56(6), 498-506.
https://doi.org/10.1007/s12033-013-9719-8

Mrkić I, Abughren M, Nikolić J, Anđelković U, Vassilopoulou E, Sinaniotis A, Petersen A, Papadopoulos NG, Gavrović-Jankulović M. Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit. in Molecular Biotechnology. 2014;56(6):498-506.
doi:10.1007/s12033-013-9719-8 .

Mrkić, Ivan, Abughren, Mohamed, Nikolić, Jasna, Anđelković, Uroš, Vassilopoulou, Emilia, Sinaniotis, Athanassios, Petersen, Arnd, Papadopoulos, Nikolaos G., Gavrović-Jankulović, Marija, "Molecular Characterization of Recombinant Mus a 5 Allergen from Banana Fruit" in Molecular Biotechnology, 56, no. 6 (2014):498-506,
https://doi.org/10.1007/s12033-013-9719-8 . .

TY  - JOUR
AU  - Klein, H.
AU  - Nikolić, Jasna
AU  - Gavrović-Jankulović, Marija
AU  - Jappe, Uta
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1426
PB  - Dustri-Verlag Dr Karl Feistle, Deisenhofen-Muenchen
T2  - Allergologie
T1  - Anaphylactic reaction to banana: genuine class I food allergy - pollen-food or latex-fruit syndrome?
VL  - 36
IS  - 9
SP  - 385
EP  - 390
DO  - 10.5414/ALX01616
ER  - 

@article{
author = "Klein, H. and Nikolić, Jasna and Gavrović-Jankulović, Marija and Jappe, Uta",
year = "2013",
publisher = "Dustri-Verlag Dr Karl Feistle, Deisenhofen-Muenchen",
journal = "Allergologie",
title = "Anaphylactic reaction to banana: genuine class I food allergy - pollen-food or latex-fruit syndrome?",
volume = "36",
number = "9",
pages = "385-390",
doi = "10.5414/ALX01616"
}

Klein, H., Nikolić, J., Gavrović-Jankulović, M.,& Jappe, U.. (2013). Anaphylactic reaction to banana: genuine class I food allergy - pollen-food or latex-fruit syndrome?. in Allergologie
Dustri-Verlag Dr Karl Feistle, Deisenhofen-Muenchen., 36(9), 385-390.
https://doi.org/10.5414/ALX01616

Klein H, Nikolić J, Gavrović-Jankulović M, Jappe U. Anaphylactic reaction to banana: genuine class I food allergy - pollen-food or latex-fruit syndrome?. in Allergologie. 2013;36(9):385-390.
doi:10.5414/ALX01616 .

Klein, H., Nikolić, Jasna, Gavrović-Jankulović, Marija, Jappe, Uta, "Anaphylactic reaction to banana: genuine class I food allergy - pollen-food or latex-fruit syndrome?" in Allergologie, 36, no. 9 (2013):385-390,
https://doi.org/10.5414/ALX01616 . .