TY  - JOUR
AU  - Rašković, Aleksandar
AU  - Martić, Nikola
AU  - Tomas, Ana
AU  - Andrejić-Višnjić, Bojana
AU  - Bosanac, Milana
AU  - Atanasković, Marko
AU  - Nemet, Marko
AU  - Popović, Radmila
AU  - Krstić, Marko
AU  - Vukmirović, Saša
AU  - Stilinović, Nebojša
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6396
AB  - Dyslipidemia and obesity are recognized as two of the major global health issues and main risk factors for coronary heart disease and cerebrovascular disease. In recent years, carob has shown certain antioxidant and anti-dyslipidemic potential. In this study, Wistar rats were fed with a standard and cholesterol-enriched diet and treated orally with carob extract and simvastatin for four weeks. After sacrifice, blood samples were collected for biochemical analysis, and liver tissue was taken for histological and immunohistochemical assessment. Weight gain was significantly higher in groups fed with cholesterol-fortified granules; total cholesterol was found to be significantly lower in the hypercholesterolemic groups treated with simvastatin and simvastatin/carob combined regimens compared with hypercholesterolemic animals treated with saline (p < 0.05). The same was true for low-density lipoprotein cholesterol and the LDL/HDL ratio (p < 0.05). Adiponectin was remarkably higher in animals treated with simvastatin compared to all other groups (p < 0.05). Leptin was significantly lower in groups treated with carob and simvastatin compared to the hypercholesterolemic group treated with saline (p < 0.05). Carob/simvastatin co-administration reduced hepatocyte damage and improved liver morphology. A study confirmed the anti-dyslipidemic, anti-obesity, and hepatoprotective potential of carob pulp alone or in combination with simvastatin in the treatment of high-fat diet-fed rats.
PB  - MDPI
T2  - Pharmaceutics
T1  - Carob Extract (Ceratonia siliqua L.): Effects on Dyslipidemia and Obesity in a High-Fat Diet-Fed Rat Model
VL  - 15
IS  - 11
SP  - 2611
DO  - 10.3390/pharmaceutics15112611
ER  - 

@article{
author = "Rašković, Aleksandar and Martić, Nikola and Tomas, Ana and Andrejić-Višnjić, Bojana and Bosanac, Milana and Atanasković, Marko and Nemet, Marko and Popović, Radmila and Krstić, Marko and Vukmirović, Saša and Stilinović, Nebojša",
year = "2023",
abstract = "Dyslipidemia and obesity are recognized as two of the major global health issues and main risk factors for coronary heart disease and cerebrovascular disease. In recent years, carob has shown certain antioxidant and anti-dyslipidemic potential. In this study, Wistar rats were fed with a standard and cholesterol-enriched diet and treated orally with carob extract and simvastatin for four weeks. After sacrifice, blood samples were collected for biochemical analysis, and liver tissue was taken for histological and immunohistochemical assessment. Weight gain was significantly higher in groups fed with cholesterol-fortified granules; total cholesterol was found to be significantly lower in the hypercholesterolemic groups treated with simvastatin and simvastatin/carob combined regimens compared with hypercholesterolemic animals treated with saline (p < 0.05). The same was true for low-density lipoprotein cholesterol and the LDL/HDL ratio (p < 0.05). Adiponectin was remarkably higher in animals treated with simvastatin compared to all other groups (p < 0.05). Leptin was significantly lower in groups treated with carob and simvastatin compared to the hypercholesterolemic group treated with saline (p < 0.05). Carob/simvastatin co-administration reduced hepatocyte damage and improved liver morphology. A study confirmed the anti-dyslipidemic, anti-obesity, and hepatoprotective potential of carob pulp alone or in combination with simvastatin in the treatment of high-fat diet-fed rats.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Carob Extract (Ceratonia siliqua L.): Effects on Dyslipidemia and Obesity in a High-Fat Diet-Fed Rat Model",
volume = "15",
number = "11",
pages = "2611",
doi = "10.3390/pharmaceutics15112611"
}

Rašković, A., Martić, N., Tomas, A., Andrejić-Višnjić, B., Bosanac, M., Atanasković, M., Nemet, M., Popović, R., Krstić, M., Vukmirović, S.,& Stilinović, N.. (2023). Carob Extract (Ceratonia siliqua L.): Effects on Dyslipidemia and Obesity in a High-Fat Diet-Fed Rat Model. in Pharmaceutics
MDPI., 15(11), 2611.
https://doi.org/10.3390/pharmaceutics15112611

Rašković A, Martić N, Tomas A, Andrejić-Višnjić B, Bosanac M, Atanasković M, Nemet M, Popović R, Krstić M, Vukmirović S, Stilinović N. Carob Extract (Ceratonia siliqua L.): Effects on Dyslipidemia and Obesity in a High-Fat Diet-Fed Rat Model. in Pharmaceutics. 2023;15(11):2611.
doi:10.3390/pharmaceutics15112611 .

Rašković, Aleksandar, Martić, Nikola, Tomas, Ana, Andrejić-Višnjić, Bojana, Bosanac, Milana, Atanasković, Marko, Nemet, Marko, Popović, Radmila, Krstić, Marko, Vukmirović, Saša, Stilinović, Nebojša, "Carob Extract (Ceratonia siliqua L.): Effects on Dyslipidemia and Obesity in a High-Fat Diet-Fed Rat Model" in Pharmaceutics, 15, no. 11 (2023):2611,
https://doi.org/10.3390/pharmaceutics15112611 . .

TY  - JOUR
AU  - Krstić, Marko
AU  - Teslić, Nemanja
AU  - Bošković, Perica
AU  - Obradović, Darija
AU  - Zeković, Zoran P.
AU  - Milić, Anita
AU  - Pavlić, Branimir
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5815
AB  - Garlic (Allium sativum L.) is widely used in various food products and traditional medicine. Besides unique taste and flavour, it is well known for its chemical profile and bioactive potential. The aim of this study was to apply subcritical water extraction (SWE) and pressurized liquid extraction (PLE) for the extraction of bioactive compounds from the Ranco genotype of garlic. Moreover, PLE process was optimized using response surface methodology (RSM) in order to determine effects and optimize ethanol concentration (45–75%), number of cycles (1–3), extraction time (1–3 min) and temperature (70–110 °C) for maximized total phenols content (TP) and antioxidant activity evaluated by various in vitro assays. Furthermore, temperature effect in SWE process on all responses was evaluated, while allicin content (AC), as a major organosulphur compound, was determined in all samples. Results indicated that PLE provided tremendous advantage over SWE in terms of improved yield and antioxidant activity of garlic extracts. Therefore, high-pressure processes could be used as clean and green procedures for the isolation of garlic bioactives.
PB  - MDPI
T2  - Molecules
T1  - Isolation of Garlic Bioactives by Pressurized Liquid and Subcritical Water Extraction
VL  - 28
IS  - 1
SP  - 369
DO  - 10.3390/molecules28010369
ER  - 

@article{
author = "Krstić, Marko and Teslić, Nemanja and Bošković, Perica and Obradović, Darija and Zeković, Zoran P. and Milić, Anita and Pavlić, Branimir",
year = "2023",
abstract = "Garlic (Allium sativum L.) is widely used in various food products and traditional medicine. Besides unique taste and flavour, it is well known for its chemical profile and bioactive potential. The aim of this study was to apply subcritical water extraction (SWE) and pressurized liquid extraction (PLE) for the extraction of bioactive compounds from the Ranco genotype of garlic. Moreover, PLE process was optimized using response surface methodology (RSM) in order to determine effects and optimize ethanol concentration (45–75%), number of cycles (1–3), extraction time (1–3 min) and temperature (70–110 °C) for maximized total phenols content (TP) and antioxidant activity evaluated by various in vitro assays. Furthermore, temperature effect in SWE process on all responses was evaluated, while allicin content (AC), as a major organosulphur compound, was determined in all samples. Results indicated that PLE provided tremendous advantage over SWE in terms of improved yield and antioxidant activity of garlic extracts. Therefore, high-pressure processes could be used as clean and green procedures for the isolation of garlic bioactives.",
publisher = "MDPI",
journal = "Molecules",
title = "Isolation of Garlic Bioactives by Pressurized Liquid and Subcritical Water Extraction",
volume = "28",
number = "1",
pages = "369",
doi = "10.3390/molecules28010369"
}

Krstić, M., Teslić, N., Bošković, P., Obradović, D., Zeković, Z. P., Milić, A.,& Pavlić, B.. (2023). Isolation of Garlic Bioactives by Pressurized Liquid and Subcritical Water Extraction. in Molecules
MDPI., 28(1), 369.
https://doi.org/10.3390/molecules28010369

Krstić M, Teslić N, Bošković P, Obradović D, Zeković ZP, Milić A, Pavlić B. Isolation of Garlic Bioactives by Pressurized Liquid and Subcritical Water Extraction. in Molecules. 2023;28(1):369.
doi:10.3390/molecules28010369 .

Krstić, Marko, Teslić, Nemanja, Bošković, Perica, Obradović, Darija, Zeković, Zoran P., Milić, Anita, Pavlić, Branimir, "Isolation of Garlic Bioactives by Pressurized Liquid and Subcritical Water Extraction" in Molecules, 28, no. 1 (2023):369,
https://doi.org/10.3390/molecules28010369 . .

TY  - JOUR
AU  - Đogo Mračević, Svetlana
AU  - Krstić, Marko
AU  - Lolić, Aleksandar
AU  - Ražić, Slavica
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4986
AB  - A chemical characterization and biological activity of 7 honey types (multifloral, linden, rapeseed, sunflower,
phacelia, acacia and honeydew honey) from different regions of Serbia were presented. The physicochemical
characteristics, mineral content, antioxidant and antimicrobial activity were estimated and discussed. All honeys
showed good nutritional characteristics, according to the adopted criteria of the standard codex for honey. The
concentrations of 15 elements in honey samples were determined using inductively coupled plasma optical
emission spectroscopy (ICP-OES). Limits of quantification were in the range from 0.003 mg/kg (Cd, Cr, Cu, Mn,
and Pb) to 1.50 mg/kg for sodium. Precision was expressed as relative standard deviation and its values were
lower than 1.50%. Accuracy was evaluated with certified reference material (fish protein, DORM 4, NRC and
cooking chocolate, SRM 2384, NIST) and obtained recovery percentages were 71-127%. The content of the most
abundant elements (K, Mg, and Na) and microelements (Al, Cu, Fe, Mn, Ni, Se, Si, and Zn) showed a significant
variability in accordance of their botanical and geographical origin, but also the influence of harvest conditions.
The levels of toxic metals (As, Cd, Cr, and Pb) are much lower than the maximal allowed for these metals
according to European Commission Regulation. The antioxidant activity was evaluated by DPPH
(Diphenylpicrylhydrazyl) assay while antimicrobial activity against Gram positive and Gram negative bacteria
(E. coli and S. aureus) and fungi Candidi albicans was estimated by microdilution assay. The free radical
scavenging assay (%RSA) varies significantly among the honey samples. The highest antioxidant activity was
observed with honeydew honeys (over 75% RSA) and the lowest was observed in the acacia, ranging from 22.96
to 24.57% RSA. All honeys tested in this study exhibited antibacterial activity, with inhibition of bacterial
growth, generally the higher against E. coli (80% for linden honeys) than against S. aureus (mostly much lower
than 50%). Activity against Candidi albicans was almost negligible. The dataset composed of all experimental
results was subjected to a chemometric evaluation and cluster analysis as hierarchical nonsupervised method
was applied. According to the obtained outcome of applied algorithms, both botanical and geographical origin
have important role in the elemental composition, antioxidant and antimicrobial activities.
PB  - Elsevier
T2  - Microchemical Journal
T1  - Comparative study of the cheical composition and biological potential of honey from different regions of Serbia
VL  - 152
SP  - 104420
DO  - 10.1016/j.microc.2019.104420
ER  - 

@article{
author = "Đogo Mračević, Svetlana and Krstić, Marko and Lolić, Aleksandar and Ražić, Slavica",
year = "2020",
abstract = "A chemical characterization and biological activity of 7 honey types (multifloral, linden, rapeseed, sunflower,
phacelia, acacia and honeydew honey) from different regions of Serbia were presented. The physicochemical
characteristics, mineral content, antioxidant and antimicrobial activity were estimated and discussed. All honeys
showed good nutritional characteristics, according to the adopted criteria of the standard codex for honey. The
concentrations of 15 elements in honey samples were determined using inductively coupled plasma optical
emission spectroscopy (ICP-OES). Limits of quantification were in the range from 0.003 mg/kg (Cd, Cr, Cu, Mn,
and Pb) to 1.50 mg/kg for sodium. Precision was expressed as relative standard deviation and its values were
lower than 1.50%. Accuracy was evaluated with certified reference material (fish protein, DORM 4, NRC and
cooking chocolate, SRM 2384, NIST) and obtained recovery percentages were 71-127%. The content of the most
abundant elements (K, Mg, and Na) and microelements (Al, Cu, Fe, Mn, Ni, Se, Si, and Zn) showed a significant
variability in accordance of their botanical and geographical origin, but also the influence of harvest conditions.
The levels of toxic metals (As, Cd, Cr, and Pb) are much lower than the maximal allowed for these metals
according to European Commission Regulation. The antioxidant activity was evaluated by DPPH
(Diphenylpicrylhydrazyl) assay while antimicrobial activity against Gram positive and Gram negative bacteria
(E. coli and S. aureus) and fungi Candidi albicans was estimated by microdilution assay. The free radical
scavenging assay (%RSA) varies significantly among the honey samples. The highest antioxidant activity was
observed with honeydew honeys (over 75% RSA) and the lowest was observed in the acacia, ranging from 22.96
to 24.57% RSA. All honeys tested in this study exhibited antibacterial activity, with inhibition of bacterial
growth, generally the higher against E. coli (80% for linden honeys) than against S. aureus (mostly much lower
than 50%). Activity against Candidi albicans was almost negligible. The dataset composed of all experimental
results was subjected to a chemometric evaluation and cluster analysis as hierarchical nonsupervised method
was applied. According to the obtained outcome of applied algorithms, both botanical and geographical origin
have important role in the elemental composition, antioxidant and antimicrobial activities.",
publisher = "Elsevier",
journal = "Microchemical Journal",
title = "Comparative study of the cheical composition and biological potential of honey from different regions of Serbia",
volume = "152",
pages = "104420",
doi = "10.1016/j.microc.2019.104420"
}

Đogo Mračević, S., Krstić, M., Lolić, A.,& Ražić, S.. (2020). Comparative study of the cheical composition and biological potential of honey from different regions of Serbia. in Microchemical Journal
Elsevier., 152, 104420.
https://doi.org/10.1016/j.microc.2019.104420

Đogo Mračević S, Krstić M, Lolić A, Ražić S. Comparative study of the cheical composition and biological potential of honey from different regions of Serbia. in Microchemical Journal. 2020;152:104420.
doi:10.1016/j.microc.2019.104420 .

Đogo Mračević, Svetlana, Krstić, Marko, Lolić, Aleksandar, Ražić, Slavica, "Comparative study of the cheical composition and biological potential of honey from different regions of Serbia" in Microchemical Journal, 152 (2020):104420,
https://doi.org/10.1016/j.microc.2019.104420 . .

TY  - JOUR
AU  - Marković, Marijana
AU  - Petrović, Jana
AU  - Đogo Mračević, Svetlana
AU  - Lolić, Aleksandar
AU  - Krstić, Marko
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4984
AB  - Uvod: Kofein je prirodni alkaloid, izolovan iz više od 60 biljnih vrsta. Preko 80 % svetske populacije
svakodnevno unosi kofein u organizam kroz konzumaciju kafe, čajeva, čokolade i, u poslednje
vreme, energetskih pića. Ne treba zanemariti činjenicu da kofein, kao farmakološki aktivna
supstanca, ulazi u sastav mnogih lekova koji se na tržištu Republike Srbije izdaju bez lekarskog
recepta. Sem toga, kofein je psihoaktivni stimulans, blag je diuretik i pokazuje interakciju sa nekim
lekovima usled indukcije CYP1 i CYP2 izoenzima, zbog čega je potrebno voditi računa o količini
koja se unese u organizam. U skladu s tim, važno je i da sadržaj kofeina u energetskim pićima odgovara
podacima koje proizvođači navode na deklaraciji.
Cilj: Cilj ovog rada bio je spektrofotometrijsko određivanje sadržaja kofeina u uzorcima energetskih
pića i čajeva proizvođača dostupnih na tržištu R. Srbije.
Materijal i metode: Kofein je određivan metodom ultraljubičaste i vidljive (UV/VIS) spektrofotometrije
u devet vrsta energetskih pića i po četiri uzorka crnog i zelenog čaja.
Rezultati: U uzorcima energetskih pića određeni sadržaj kofeina je u opsegu od 26,81 do 35,44
mg/100 mL i u skladu je s deklarisanim sadržajem. Sadržaj kofeina u uzorcima čajeva varira u
odnosu na vrstu (11,27-22,61 mg/100 mL zeleni i 13,85-22,73 mg/100 mL crni), ali je vezan i za
poreklo, uslove gajenja i proces prerade čajeva.
Zaključak: Ultraljubičasta i vidljiva spektrofotometrija se pokazala kao brza, precizna i jednostavna
metoda za određivanje kofeina u energetskim pićima i čajevima. Imajući u vidu dobijene rezultate,
važno je istaći da unos kofeina kroz uobičajenu dnevnu konzumaciju čajeva i energetskih pića
ne bi trebalo da utiče negativno na zdravlje ljudi. Svakako treba imati u vidu da se ovaj alkaloid
dodatno može uneti i putem drugih izvora, što je posebno važno za određene grupe pacijenata.
AB  - Introduction: Caffeine is a natural alkaloid, isolated from more than 60 plant species. Over 80%  of  the  world’s  population  consumes  caffeine  on  daily  basis  through  the  consumption  of  coffee,  tea,  chocolates  and,  more  recently,  energy  drinks.  As  a  pharmacologically  potent  substance  caffeine  is  an  ingredient  of  many  medicaments  available  on  the  pharmaceutical  market of Republic of Serbia without a doctor’s prescription. Besides that, caffeine acts as a psychoactive  stimulant,  mild  diuretic,  and  displays  an  interaction  with  some  medicaments  due  to  the  induction  of  CYP1  and  CYP2  isoenzymes.  Keeping  in  mind  all  of  the  above,  it  is necessary to take into account the daily caffeine intake, as well as to control its content in energy drinks.Aim:  The  aim  of  this  study  was  spectrophotometric  determination  of  caffeine  content  in  energy drinks and tea, available in markets of the Republic of Serbia. Material  and  Methods:  Caffeine  was  determinated  by  UV/VIS  spectrophotometry  in  nine  types of energy drinks and eight samples of black and green tea. Results: The results showed that caffeine content in energy drink samples ranged from 26.81 to 35.44 mg/100mL and corresponds to declaration. The caffeine content in tea samples varies with the species (11.27-22.61 mg/100mL for green and 13.85-22.73 mg/100mL for black tea), but also with origin, growth conditions and tea processing.Conclusion: The obtained data suggest that UV/VIS spectrophotometry can be used for ro-utine determination and control of caffeine content in energy drinks and tea. It is important to  note  that  caffeine  intake  through  the  usual  daily  consumption  of  tea  and  energy  drinks  should not have a negative impact on human health, but this alkaloid could be additionally consumed through other sources, which is especially important for certain groups of patients.
PB  - Beograd : Medicinski fakultet
T2  - Medicinski podmladak
T1  - Spectrophotometric determination of caffeine in energy drinks and teas
T1  - Spektrofotometrijsko određivanje sadržaja kofeina  u energetskim pićima i čajevima
VL  - 71
IS  - 3
SP  - 8
DO  - 10.5937/mp71-26638
ER  - 

@article{
author = "Marković, Marijana and Petrović, Jana and Đogo Mračević, Svetlana and Lolić, Aleksandar and Krstić, Marko",
year = "2020",
abstract = "Uvod: Kofein je prirodni alkaloid, izolovan iz više od 60 biljnih vrsta. Preko 80 % svetske populacije
svakodnevno unosi kofein u organizam kroz konzumaciju kafe, čajeva, čokolade i, u poslednje
vreme, energetskih pića. Ne treba zanemariti činjenicu da kofein, kao farmakološki aktivna
supstanca, ulazi u sastav mnogih lekova koji se na tržištu Republike Srbije izdaju bez lekarskog
recepta. Sem toga, kofein je psihoaktivni stimulans, blag je diuretik i pokazuje interakciju sa nekim
lekovima usled indukcije CYP1 i CYP2 izoenzima, zbog čega je potrebno voditi računa o količini
koja se unese u organizam. U skladu s tim, važno je i da sadržaj kofeina u energetskim pićima odgovara
podacima koje proizvođači navode na deklaraciji.
Cilj: Cilj ovog rada bio je spektrofotometrijsko određivanje sadržaja kofeina u uzorcima energetskih
pića i čajeva proizvođača dostupnih na tržištu R. Srbije.
Materijal i metode: Kofein je određivan metodom ultraljubičaste i vidljive (UV/VIS) spektrofotometrije
u devet vrsta energetskih pića i po četiri uzorka crnog i zelenog čaja.
Rezultati: U uzorcima energetskih pića određeni sadržaj kofeina je u opsegu od 26,81 do 35,44
mg/100 mL i u skladu je s deklarisanim sadržajem. Sadržaj kofeina u uzorcima čajeva varira u
odnosu na vrstu (11,27-22,61 mg/100 mL zeleni i 13,85-22,73 mg/100 mL crni), ali je vezan i za
poreklo, uslove gajenja i proces prerade čajeva.
Zaključak: Ultraljubičasta i vidljiva spektrofotometrija se pokazala kao brza, precizna i jednostavna
metoda za određivanje kofeina u energetskim pićima i čajevima. Imajući u vidu dobijene rezultate,
važno je istaći da unos kofeina kroz uobičajenu dnevnu konzumaciju čajeva i energetskih pića
ne bi trebalo da utiče negativno na zdravlje ljudi. Svakako treba imati u vidu da se ovaj alkaloid
dodatno može uneti i putem drugih izvora, što je posebno važno za određene grupe pacijenata., Introduction: Caffeine is a natural alkaloid, isolated from more than 60 plant species. Over 80%  of  the  world’s  population  consumes  caffeine  on  daily  basis  through  the  consumption  of  coffee,  tea,  chocolates  and,  more  recently,  energy  drinks.  As  a  pharmacologically  potent  substance  caffeine  is  an  ingredient  of  many  medicaments  available  on  the  pharmaceutical  market of Republic of Serbia without a doctor’s prescription. Besides that, caffeine acts as a psychoactive  stimulant,  mild  diuretic,  and  displays  an  interaction  with  some  medicaments  due  to  the  induction  of  CYP1  and  CYP2  isoenzymes.  Keeping  in  mind  all  of  the  above,  it  is necessary to take into account the daily caffeine intake, as well as to control its content in energy drinks.Aim:  The  aim  of  this  study  was  spectrophotometric  determination  of  caffeine  content  in  energy drinks and tea, available in markets of the Republic of Serbia. Material  and  Methods:  Caffeine  was  determinated  by  UV/VIS  spectrophotometry  in  nine  types of energy drinks and eight samples of black and green tea. Results: The results showed that caffeine content in energy drink samples ranged from 26.81 to 35.44 mg/100mL and corresponds to declaration. The caffeine content in tea samples varies with the species (11.27-22.61 mg/100mL for green and 13.85-22.73 mg/100mL for black tea), but also with origin, growth conditions and tea processing.Conclusion: The obtained data suggest that UV/VIS spectrophotometry can be used for ro-utine determination and control of caffeine content in energy drinks and tea. It is important to  note  that  caffeine  intake  through  the  usual  daily  consumption  of  tea  and  energy  drinks  should not have a negative impact on human health, but this alkaloid could be additionally consumed through other sources, which is especially important for certain groups of patients.",
publisher = "Beograd : Medicinski fakultet",
journal = "Medicinski podmladak",
title = "Spectrophotometric determination of caffeine in energy drinks and teas, Spektrofotometrijsko određivanje sadržaja kofeina  u energetskim pićima i čajevima",
volume = "71",
number = "3",
pages = "8",
doi = "10.5937/mp71-26638"
}

Marković, M., Petrović, J., Đogo Mračević, S., Lolić, A.,& Krstić, M.. (2020). Spectrophotometric determination of caffeine in energy drinks and teas. in Medicinski podmladak
Beograd : Medicinski fakultet., 71(3), 8.
https://doi.org/10.5937/mp71-26638

Marković M, Petrović J, Đogo Mračević S, Lolić A, Krstić M. Spectrophotometric determination of caffeine in energy drinks and teas. in Medicinski podmladak. 2020;71(3):8.
doi:10.5937/mp71-26638 .

Marković, Marijana, Petrović, Jana, Đogo Mračević, Svetlana, Lolić, Aleksandar, Krstić, Marko, "Spectrophotometric determination of caffeine in energy drinks and teas" in Medicinski podmladak, 71, no. 3 (2020):8,
https://doi.org/10.5937/mp71-26638 . .

TY  - JOUR
AU  - Đogo Mračević, Svetlana
AU  - Krstić, Marko
AU  - Lolić, Aleksandar
AU  - Ražić, Slavica
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4995
AB  - A chemical characterization and biological activity of 7 honey types (multifloral, linden, rapeseed, sunflower,phacelia, acacia and honeydew honey) from different regions of Serbia were presented. The physicochemicalcharacteristics, mineral content, antioxidant and antimicrobial activity were estimated and discussed. All honeysshowed good nutritional characteristics, according to the adopted criteria of the standard codex for honey. Theconcentrations of 15 elements in honey samples were determined using inductively coupled plasma opticalemission spectroscopy (ICP-OES). Limits of quantification were in the range from 0.003 mg/kg (Cd, Cr, Cu, Mn,and Pb) to 1.50 mg/kg for sodium. Precision was expressed as relative standard deviation and its values werelower than 1.50%. Accuracy was evaluated with certified reference material (fish protein, DORM 4, NRC andcooking chocolate, SRM 2384, NIST) and obtained recovery percentages were 71-127%. The content of the mostabundant elements (K, Mg, and Na) and microelements (Al, Cu, Fe, Mn, Ni, Se, Si, and Zn) showed a significantvariability in accordance of their botanical and geographical origin, but also the influence of harvest conditions.The levels of toxic metals (As, Cd, Cr, and Pb) are much lower than the maximal allowed for these metalsaccording to European Commission Regulation. The antioxidant activity was evaluated by DPPH(Diphenylpicrylhydrazyl) assay while antimicrobial activity against Gram positive and Gram negative bacteria(E. coli and S. aureus) and fungi Candidi albicans was estimated by microdilution assay. The free radicalscavenging assay (%RSA) varies significantly among the honey samples. The highest antioxidant activity wasobserved with honeydew honeys (over 75% RSA) and the lowest was observed in the acacia, ranging from 22.96to 24.57% RSA. All honeys tested in this study exhibited antibacterial activity, with inhibition of bacterialgrowth, generally the higher against E. coli (80% for linden honeys) than against S. aureus (mostly much lowerthan 50%). Activity against Candidi albicans was almost negligible. The dataset composed of all experimentalresults was subjected to a chemometric evaluation and cluster analysis as hierarchical nonsupervised methodwas applied. According to the obtained outcome of applied algorithms, both botanical and geographical originhave important role in the elemental composition, antioxidant and antimicrobial activities.
PB  - Elsevier
T2  - Microchemical Journal
T1  - Comparative study of the cheical composition and biological potential of honey from different regions of Serbia
VL  - 152
SP  - 104420
DO  - 10.1016/j.microc.2019.104420
ER  - 

@article{
author = "Đogo Mračević, Svetlana and Krstić, Marko and Lolić, Aleksandar and Ražić, Slavica",
year = "2020",
abstract = "A chemical characterization and biological activity of 7 honey types (multifloral, linden, rapeseed, sunflower,phacelia, acacia and honeydew honey) from different regions of Serbia were presented. The physicochemicalcharacteristics, mineral content, antioxidant and antimicrobial activity were estimated and discussed. All honeysshowed good nutritional characteristics, according to the adopted criteria of the standard codex for honey. Theconcentrations of 15 elements in honey samples were determined using inductively coupled plasma opticalemission spectroscopy (ICP-OES). Limits of quantification were in the range from 0.003 mg/kg (Cd, Cr, Cu, Mn,and Pb) to 1.50 mg/kg for sodium. Precision was expressed as relative standard deviation and its values werelower than 1.50%. Accuracy was evaluated with certified reference material (fish protein, DORM 4, NRC andcooking chocolate, SRM 2384, NIST) and obtained recovery percentages were 71-127%. The content of the mostabundant elements (K, Mg, and Na) and microelements (Al, Cu, Fe, Mn, Ni, Se, Si, and Zn) showed a significantvariability in accordance of their botanical and geographical origin, but also the influence of harvest conditions.The levels of toxic metals (As, Cd, Cr, and Pb) are much lower than the maximal allowed for these metalsaccording to European Commission Regulation. The antioxidant activity was evaluated by DPPH(Diphenylpicrylhydrazyl) assay while antimicrobial activity against Gram positive and Gram negative bacteria(E. coli and S. aureus) and fungi Candidi albicans was estimated by microdilution assay. The free radicalscavenging assay (%RSA) varies significantly among the honey samples. The highest antioxidant activity wasobserved with honeydew honeys (over 75% RSA) and the lowest was observed in the acacia, ranging from 22.96to 24.57% RSA. All honeys tested in this study exhibited antibacterial activity, with inhibition of bacterialgrowth, generally the higher against E. coli (80% for linden honeys) than against S. aureus (mostly much lowerthan 50%). Activity against Candidi albicans was almost negligible. The dataset composed of all experimentalresults was subjected to a chemometric evaluation and cluster analysis as hierarchical nonsupervised methodwas applied. According to the obtained outcome of applied algorithms, both botanical and geographical originhave important role in the elemental composition, antioxidant and antimicrobial activities.",
publisher = "Elsevier",
journal = "Microchemical Journal",
title = "Comparative study of the cheical composition and biological potential of honey from different regions of Serbia",
volume = "152",
pages = "104420",
doi = "10.1016/j.microc.2019.104420"
}

Đogo Mračević, S., Krstić, M., Lolić, A.,& Ražić, S.. (2020). Comparative study of the cheical composition and biological potential of honey from different regions of Serbia. in Microchemical Journal
Elsevier., 152, 104420.
https://doi.org/10.1016/j.microc.2019.104420

Đogo Mračević S, Krstić M, Lolić A, Ražić S. Comparative study of the cheical composition and biological potential of honey from different regions of Serbia. in Microchemical Journal. 2020;152:104420.
doi:10.1016/j.microc.2019.104420 .

Đogo Mračević, Svetlana, Krstić, Marko, Lolić, Aleksandar, Ražić, Slavica, "Comparative study of the cheical composition and biological potential of honey from different regions of Serbia" in Microchemical Journal, 152 (2020):104420,
https://doi.org/10.1016/j.microc.2019.104420 . .

TY  - DATA
AU  - Đogo Mračević, Svetlana
AU  - Krstić, Marko
AU  - Lolić, Aleksandar
AU  - Ražić, Slavica
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4996
PB  - Elsevier
T2  - Microchemical Journal
T1  - Supplementary data for the article: Đogo Mračević, S.; Krstić, M.; Lolić, A.; Ražić, S. Comparative Study of the Cheical Composition and Biological Potential of Honey from Different Regions of Serbia. Microchemical Journal 2020, 152, 104420. https://doi.org/10.1016/j.microc.2019.104420.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4996
ER  - 

@misc{
author = "Đogo Mračević, Svetlana and Krstić, Marko and Lolić, Aleksandar and Ražić, Slavica",
year = "2020",
publisher = "Elsevier",
journal = "Microchemical Journal",
title = "Supplementary data for the article: Đogo Mračević, S.; Krstić, M.; Lolić, A.; Ražić, S. Comparative Study of the Cheical Composition and Biological Potential of Honey from Different Regions of Serbia. Microchemical Journal 2020, 152, 104420. https://doi.org/10.1016/j.microc.2019.104420.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4996"
}

Đogo Mračević, S., Krstić, M., Lolić, A.,& Ražić, S.. (2020). Supplementary data for the article: Đogo Mračević, S.; Krstić, M.; Lolić, A.; Ražić, S. Comparative Study of the Cheical Composition and Biological Potential of Honey from Different Regions of Serbia. Microchemical Journal 2020, 152, 104420. https://doi.org/10.1016/j.microc.2019.104420.. in Microchemical Journal
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_4996

Đogo Mračević S, Krstić M, Lolić A, Ražić S. Supplementary data for the article: Đogo Mračević, S.; Krstić, M.; Lolić, A.; Ražić, S. Comparative Study of the Cheical Composition and Biological Potential of Honey from Different Regions of Serbia. Microchemical Journal 2020, 152, 104420. https://doi.org/10.1016/j.microc.2019.104420.. in Microchemical Journal. 2020;.
https://hdl.handle.net/21.15107/rcub_cherry_4996 .

Đogo Mračević, Svetlana, Krstić, Marko, Lolić, Aleksandar, Ražić, Slavica, "Supplementary data for the article: Đogo Mračević, S.; Krstić, M.; Lolić, A.; Ražić, S. Comparative Study of the Cheical Composition and Biological Potential of Honey from Different Regions of Serbia. Microchemical Journal 2020, 152, 104420. https://doi.org/10.1016/j.microc.2019.104420." in Microchemical Journal (2020),
https://hdl.handle.net/21.15107/rcub_cherry_4996 .