TY  - CHAP
AU  - Nikolić, Milan
AU  - Minić, Simeon L.
AU  - Mačvanin, Mirjana T.
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
AU  - Jacob-Lopes, Eduardo
AU  - Queiroz, Maria Isabel
AU  - Zepka, Leila Queiroz
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4893
AB  - The aim of this chapter is to review and discuss methodology and protocols in the analysis of phycobiliproteins (phycocyanins, allophycocyanins, and phycoerythrins) and their chromophores. Due to the presence of multiple covalently bound open-chain tetrapyrrole chromophores, phycobiliproteins are colored and strongly fluorescent molecules, with high absorption coefficients (105 to 106) and excellent fluorescent quantum yield (0.51 up to 0.98). Therefore, a vast number of methods for phycobiliproteins analysis is based on these spectral characteristics, whereas assessment of their bioactivity is related to their exceptional redox and metal-chelating properties. This chapter is dedicated to methods used for isolation and purification, structure analysis, physicochemical properties and stability characterization, quantification, as well as in vitro and in vivo biological activities evaluation. In addition, emerging approaches related to phycobiliproteins analysis are also reviewed including interactions with other biomolecules and ions and identification of phycobiliprotein (chromo)peptides by mass spectrometry.
PB  - Springer International Publishing
T2  - Pigments from Microalgae Handbook
T1  - Analytical Protocols in Phycobiliproteins Analysis
SP  - 179
EP  - 201
DO  - 10.1007/978-3-030-50971-2_8
ER  - 

@inbook{
author = "Nikolić, Milan and Minić, Simeon L. and Mačvanin, Mirjana T. and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja and Jacob-Lopes, Eduardo and Queiroz, Maria Isabel and Zepka, Leila Queiroz",
year = "2020",
abstract = "The aim of this chapter is to review and discuss methodology and protocols in the analysis of phycobiliproteins (phycocyanins, allophycocyanins, and phycoerythrins) and their chromophores. Due to the presence of multiple covalently bound open-chain tetrapyrrole chromophores, phycobiliproteins are colored and strongly fluorescent molecules, with high absorption coefficients (105 to 106) and excellent fluorescent quantum yield (0.51 up to 0.98). Therefore, a vast number of methods for phycobiliproteins analysis is based on these spectral characteristics, whereas assessment of their bioactivity is related to their exceptional redox and metal-chelating properties. This chapter is dedicated to methods used for isolation and purification, structure analysis, physicochemical properties and stability characterization, quantification, as well as in vitro and in vivo biological activities evaluation. In addition, emerging approaches related to phycobiliproteins analysis are also reviewed including interactions with other biomolecules and ions and identification of phycobiliprotein (chromo)peptides by mass spectrometry.",
publisher = "Springer International Publishing",
journal = "Pigments from Microalgae Handbook",
booktitle = "Analytical Protocols in Phycobiliproteins Analysis",
pages = "179-201",
doi = "10.1007/978-3-030-50971-2_8"
}

Nikolić, M., Minić, S. L., Mačvanin, M. T., Stanić-Vučinić, D., Ćirković-Veličković, T., Jacob-Lopes, E., Queiroz, M. I.,& Zepka, L. Q.. (2020). Analytical Protocols in Phycobiliproteins Analysis. in Pigments from Microalgae Handbook
Springer International Publishing., 179-201.
https://doi.org/10.1007/978-3-030-50971-2_8

Nikolić M, Minić SL, Mačvanin MT, Stanić-Vučinić D, Ćirković-Veličković T, Jacob-Lopes E, Queiroz MI, Zepka LQ. Analytical Protocols in Phycobiliproteins Analysis. in Pigments from Microalgae Handbook. 2020;:179-201.
doi:10.1007/978-3-030-50971-2_8 .

Nikolić, Milan, Minić, Simeon L., Mačvanin, Mirjana T., Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, Jacob-Lopes, Eduardo, Queiroz, Maria Isabel, Zepka, Leila Queiroz, "Analytical Protocols in Phycobiliproteins Analysis" in Pigments from Microalgae Handbook (2020):179-201,
https://doi.org/10.1007/978-3-030-50971-2_8 . .

TY  - CHAP
AU  - Nikolić, Milan
AU  - Minić, Simeon L.
AU  - Mačvanin, Mirjana T.
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
AU  - Jacob-Lopes, Eduardo
AU  - Queiroz, Maria Isabel
AU  - Zepka, Leila Queiroz
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4892
AB  - The aim of this chapter is to review and discuss methodology and protocols in the analysis of phycobiliproteins (phycocyanins, allophycocyanins, and phycoerythrins) and their chromophores. Due to the presence of multiple covalently bound open-chain tetrapyrrole chromophores, phycobiliproteins are colored and strongly fluorescent molecules, with high absorption coefficients (105 to 106) and excellent fluorescent quantum yield (0.51 up to 0.98). Therefore, a vast number of methods for phycobiliproteins analysis is based on these spectral characteristics, whereas assessment of their bioactivity is related to their exceptional redox and metal-chelating properties. This chapter is dedicated to methods used for isolation and purification, structure analysis, physicochemical properties and stability characterization, quantification, as well as in vitro and in vivo biological activities evaluation. In addition, emerging approaches related to phycobiliproteins analysis are also reviewed including interactions with other biomolecules and ions and identification of phycobiliprotein (chromo)peptides by mass spectrometry.
PB  - Springer International Publishing
T2  - Pigments from Microalgae Handbook
T1  - Analytical Protocols in Phycobiliproteins Analysis
SP  - 179
EP  - 201
DO  - 10.1007/978-3-030-50971-2_8
ER  - 

@inbook{
author = "Nikolić, Milan and Minić, Simeon L. and Mačvanin, Mirjana T. and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja and Jacob-Lopes, Eduardo and Queiroz, Maria Isabel and Zepka, Leila Queiroz",
year = "2020",
abstract = "The aim of this chapter is to review and discuss methodology and protocols in the analysis of phycobiliproteins (phycocyanins, allophycocyanins, and phycoerythrins) and their chromophores. Due to the presence of multiple covalently bound open-chain tetrapyrrole chromophores, phycobiliproteins are colored and strongly fluorescent molecules, with high absorption coefficients (105 to 106) and excellent fluorescent quantum yield (0.51 up to 0.98). Therefore, a vast number of methods for phycobiliproteins analysis is based on these spectral characteristics, whereas assessment of their bioactivity is related to their exceptional redox and metal-chelating properties. This chapter is dedicated to methods used for isolation and purification, structure analysis, physicochemical properties and stability characterization, quantification, as well as in vitro and in vivo biological activities evaluation. In addition, emerging approaches related to phycobiliproteins analysis are also reviewed including interactions with other biomolecules and ions and identification of phycobiliprotein (chromo)peptides by mass spectrometry.",
publisher = "Springer International Publishing",
journal = "Pigments from Microalgae Handbook",
booktitle = "Analytical Protocols in Phycobiliproteins Analysis",
pages = "179-201",
doi = "10.1007/978-3-030-50971-2_8"
}

Nikolić, M., Minić, S. L., Mačvanin, M. T., Stanić-Vučinić, D., Ćirković-Veličković, T., Jacob-Lopes, E., Queiroz, M. I.,& Zepka, L. Q.. (2020). Analytical Protocols in Phycobiliproteins Analysis. in Pigments from Microalgae Handbook
Springer International Publishing., 179-201.
https://doi.org/10.1007/978-3-030-50971-2_8

Nikolić M, Minić SL, Mačvanin MT, Stanić-Vučinić D, Ćirković-Veličković T, Jacob-Lopes E, Queiroz MI, Zepka LQ. Analytical Protocols in Phycobiliproteins Analysis. in Pigments from Microalgae Handbook. 2020;:179-201.
doi:10.1007/978-3-030-50971-2_8 .

Nikolić, Milan, Minić, Simeon L., Mačvanin, Mirjana T., Stanić-Vučinić, Dragana, Ćirković-Veličković, Tanja, Jacob-Lopes, Eduardo, Queiroz, Maria Isabel, Zepka, Leila Queiroz, "Analytical Protocols in Phycobiliproteins Analysis" in Pigments from Microalgae Handbook (2020):179-201,
https://doi.org/10.1007/978-3-030-50971-2_8 . .