@article{
author = "Sajewicz, Mieczyslaw and Staszek, Dorota and Natić, Maja and Wojtal, Lukasz and Waksmundzka-Hajnos, Monika and Kowalska, Teresa",
year = "2011",
abstract = "In the previous paper from this series, we proposed mass spectrometric fingerprinting of a complex and volatile botanical sample upon an example of the essential oil derived from Salvia lavandulifolia. In that paper, we compared two variants of fractionation of such a mixture. A simpler one-dimensional variant consisted of the low-temperature thin-layer chromatographic fractionation coupled with mass spectrometric fingerprinting of each separated fraction (1D LT TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the low-temperature thin-layer chromatography, high-performance liquid chromatography, and mass spectrometric detection (2D LT TLC-LC-MS). In this study, we present an analogous approach to the non-volatile botanical mixtures upon an example of the pharmacologically important phenolic acids and flavonoids selectively extracted from Salvia lavandulifolia. With these non-volatile fractions, the thin-layer chromatographic separations were carried out at ambient temperature (21 +/- 0.5 degrees C). Once again, we compared two variants of fractionation. A simpler one-dimensional variant consisted of the thin-layer chromatographic mode coupled with mass spectrometric fingerprinting of each separated fraction (1D TLC-MS). A more sophisticated variant was the two-dimensional liquid chromatographic system composed of the thin-layer chromatography and high-performance liquid chromatography, with mass spectrometric detection (2D TLC-LC-MS). As expected, the two-dimensional mode proved better performing than the one-dimensional mode (1D TLC-MS). It was concluded that thin-layer chromatography directly or indirectly coupled with mass spectrometric detection can prove very useful in the analysis of the phenolic acid and flavonoid fraction selectively extracted from botanical material.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Liquid Chromatography and Related Technologies",
title = "Tlc-Ms Versus Tlc-Lc-Ms Fingerprints of Herbal Extracts. Part Ii. Phenolic Acids and Flavonoids",
volume = "34",
number = "10-11",
pages = "864-887",
doi = "10.1080/10826076.2011.571131"
}