TY  - CHAP
AU  - Lopandić, Zorana
AU  - Protić-Rosić, Isidora
AU  - Trbojević-Ivić, Jovana
AU  - Zlatanova, Milena
AU  - Gavrović-Jankulović, Marija
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6340
AB  - Ovalbumin (OVA) is the most abundant egg white protein. It is a globular, acidic phosphorylated glycoprotein of the serpin family with a molecular weight of 45 kDa. OVA is rich in essential amino acids and upon proteolytic digestion yields bioactive peptides (BAPs), recognized nutraceuticals with hypotensive, antimicrobial, antioxidant, and anticancer properties that contribute to the overall nutritional and health benefits of eggs. OVA is a common choice in the food, biomedical, and pharmaceutical industries due to its useful properties during food processing, capacity to form biocompatible gels, and special properties as an effective transporter for a variety of nutraceuticals and pharmaceuticals. Cellular agriculture is an innovative interdisciplinary approach that bypasses conventional animal husbandry in the production of animal proteins. OVA expressed in Trichoderma reesei (T. reesei) most closely mimics the structural and functional properties of its natural homolog and is therefore considered a sustainable alternative to chicken egg white protein powder.
Egg allergy poses serious concerns for food safety and an important socioeconomic burden to the food sector and public health. OVA has been extensively studied as an important egg allergen in mice and in vitro experimental models, providing fundamental insights into the molecular mechanisms of allergy and identifying new therapeutic targets. This chapter focuses on providing a comprehensive overview of the state-of-the-art of OVA in human nutrition and the food industry. After presenting the structure underlying the functional properties of OVA, we provide a critical perspective on cellular agriculture as a non-poultry production of OVA. Additionally, the detailed nutritional and biotechnological significance of OVA is elaborated. The final part of this chapter provides a comprehensive insight into OVA as a model antigen and food allergen from a food safety perspective.
PB  - Nova Science Publishers, Inc.
T2  - Advances in Health and Disease
T1  - Ovalbumin - Two Sides of the Same Coin
VL  - 74
SP  - 43
EP  - 100
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6340
ER  - 

@inbook{
author = "Lopandić, Zorana and Protić-Rosić, Isidora and Trbojević-Ivić, Jovana and Zlatanova, Milena and Gavrović-Jankulović, Marija",
year = "2023",
abstract = "Ovalbumin (OVA) is the most abundant egg white protein. It is a globular, acidic phosphorylated glycoprotein of the serpin family with a molecular weight of 45 kDa. OVA is rich in essential amino acids and upon proteolytic digestion yields bioactive peptides (BAPs), recognized nutraceuticals with hypotensive, antimicrobial, antioxidant, and anticancer properties that contribute to the overall nutritional and health benefits of eggs. OVA is a common choice in the food, biomedical, and pharmaceutical industries due to its useful properties during food processing, capacity to form biocompatible gels, and special properties as an effective transporter for a variety of nutraceuticals and pharmaceuticals. Cellular agriculture is an innovative interdisciplinary approach that bypasses conventional animal husbandry in the production of animal proteins. OVA expressed in Trichoderma reesei (T. reesei) most closely mimics the structural and functional properties of its natural homolog and is therefore considered a sustainable alternative to chicken egg white protein powder.
Egg allergy poses serious concerns for food safety and an important socioeconomic burden to the food sector and public health. OVA has been extensively studied as an important egg allergen in mice and in vitro experimental models, providing fundamental insights into the molecular mechanisms of allergy and identifying new therapeutic targets. This chapter focuses on providing a comprehensive overview of the state-of-the-art of OVA in human nutrition and the food industry. After presenting the structure underlying the functional properties of OVA, we provide a critical perspective on cellular agriculture as a non-poultry production of OVA. Additionally, the detailed nutritional and biotechnological significance of OVA is elaborated. The final part of this chapter provides a comprehensive insight into OVA as a model antigen and food allergen from a food safety perspective.",
publisher = "Nova Science Publishers, Inc.",
journal = "Advances in Health and Disease",
booktitle = "Ovalbumin - Two Sides of the Same Coin",
volume = "74",
pages = "43-100",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6340"
}

Lopandić, Z., Protić-Rosić, I., Trbojević-Ivić, J., Zlatanova, M.,& Gavrović-Jankulović, M.. (2023). Ovalbumin - Two Sides of the Same Coin. in Advances in Health and Disease
Nova Science Publishers, Inc.., 74, 43-100.
https://hdl.handle.net/21.15107/rcub_cherry_6340

Lopandić Z, Protić-Rosić I, Trbojević-Ivić J, Zlatanova M, Gavrović-Jankulović M. Ovalbumin - Two Sides of the Same Coin. in Advances in Health and Disease. 2023;74:43-100.
https://hdl.handle.net/21.15107/rcub_cherry_6340 .

Lopandić, Zorana, Protić-Rosić, Isidora, Trbojević-Ivić, Jovana, Zlatanova, Milena, Gavrović-Jankulović, Marija, "Ovalbumin - Two Sides of the Same Coin" in Advances in Health and Disease, 74 (2023):43-100,
https://hdl.handle.net/21.15107/rcub_cherry_6340 .

TY  - JOUR
AU  - Knežević, Sara
AU  - Ognjanović, Miloš
AU  - Stanković, Vesna
AU  - Zlatanova, Milena
AU  - Nešić, Andrijana N.
AU  - Gavrović-Jankulović, Marija
AU  - Stanković, Dalibor
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5622
AB  - This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.
PB  - MDPI
T2  - Biosensors
T1  - La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells
VL  - 12
IS  - 9
SP  - 705
DO  - 10.3390/bios12090705
ER  - 

@article{
author = "Knežević, Sara and Ognjanović, Miloš and Stanković, Vesna and Zlatanova, Milena and Nešić, Andrijana N. and Gavrović-Jankulović, Marija and Stanković, Dalibor",
year = "2022",
abstract = "This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.",
publisher = "MDPI",
journal = "Biosensors",
title = "La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells",
volume = "12",
number = "9",
pages = "705",
doi = "10.3390/bios12090705"
}

Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A. N., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors
MDPI., 12(9), 705.
https://doi.org/10.3390/bios12090705

Knežević S, Ognjanović M, Stanković V, Zlatanova M, Nešić AN, Gavrović-Jankulović M, Stanković D. La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors. 2022;12(9):705.
doi:10.3390/bios12090705 .

Knežević, Sara, Ognjanović, Miloš, Stanković, Vesna, Zlatanova, Milena, Nešić, Andrijana N., Gavrović-Jankulović, Marija, Stanković, Dalibor, "La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells" in Biosensors, 12, no. 9 (2022):705,
https://doi.org/10.3390/bios12090705 . .

TY  - DATA
AU  - Knežević, Sara
AU  - Ognjanović, Miloš
AU  - Stanković, Vesna
AU  - Zlatanova, Milena
AU  - Nešić, Andrijana N.
AU  - Gavrović-Jankulović, Marija
AU  - Stanković, Dalibor
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5632
AB  - This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.
PB  - MDPI
T2  - Biosensors
T1  - Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705
VL  - 12
IS  - 9
SP  - 705
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5632
ER  - 

@misc{
author = "Knežević, Sara and Ognjanović, Miloš and Stanković, Vesna and Zlatanova, Milena and Nešić, Andrijana N. and Gavrović-Jankulović, Marija and Stanković, Dalibor",
year = "2022",
abstract = "This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.",
publisher = "MDPI",
journal = "Biosensors",
title = "Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705",
volume = "12",
number = "9",
pages = "705",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5632"
}

Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A. N., Gavrović-Jankulović, M.,& Stanković, D.. (2022). Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705. in Biosensors
MDPI., 12(9), 705.
https://hdl.handle.net/21.15107/rcub_cherry_5632

Knežević S, Ognjanović M, Stanković V, Zlatanova M, Nešić AN, Gavrović-Jankulović M, Stanković D. Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705. in Biosensors. 2022;12(9):705.
https://hdl.handle.net/21.15107/rcub_cherry_5632 .

Knežević, Sara, Ognjanović, Miloš, Stanković, Vesna, Zlatanova, Milena, Nešić, Andrijana N., Gavrović-Jankulović, Marija, Stanković, Dalibor, "Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705" in Biosensors, 12, no. 9 (2022):705,
https://hdl.handle.net/21.15107/rcub_cherry_5632 .

TY  - CONF
AU  - Zlatanova, Milena
AU  - Nešić, Andrijana N.
AU  - Trbojević-Ivić, Jovana
AU  - Gavrović-Jankulović, Marija
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5167
PB  - Belgrade : Faculty of Chemistry
C3  - Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia
T1  - Small molecules attenuate activation of the NF-kB signaling in epithelial cells by Act d 1 kiwifruit allergen
SP  - 186
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5167
ER  - 

@conference{
author = "Zlatanova, Milena and Nešić, Andrijana N. and Trbojević-Ivić, Jovana and Gavrović-Jankulović, Marija",
year = "2021",
publisher = "Belgrade : Faculty of Chemistry",
journal = "Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia",
title = "Small molecules attenuate activation of the NF-kB signaling in epithelial cells by Act d 1 kiwifruit allergen",
pages = "186",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5167"
}

Zlatanova, M., Nešić, A. N., Trbojević-Ivić, J.,& Gavrović-Jankulović, M.. (2021). Small molecules attenuate activation of the NF-kB signaling in epithelial cells by Act d 1 kiwifruit allergen. in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia
Belgrade : Faculty of Chemistry., 186.
https://hdl.handle.net/21.15107/rcub_cherry_5167

Zlatanova M, Nešić AN, Trbojević-Ivić J, Gavrović-Jankulović M. Small molecules attenuate activation of the NF-kB signaling in epithelial cells by Act d 1 kiwifruit allergen. in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia. 2021;:186.
https://hdl.handle.net/21.15107/rcub_cherry_5167 .

Zlatanova, Milena, Nešić, Andrijana N., Trbojević-Ivić, Jovana, Gavrović-Jankulović, Marija, "Small molecules attenuate activation of the NF-kB signaling in epithelial cells by Act d 1 kiwifruit allergen" in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia (2021):186,
https://hdl.handle.net/21.15107/rcub_cherry_5167 .

TY  - THES
AU  - Zlatanova, Milena
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4016
AB  - Alergijske reakcije na hranu predstavljaju reakciju imunog sistema posredovanu IgE antitelima na proteine poreklom iz hrane. Epitelne ćelije gastrointestinalnog trakta predstavljaju prvu barijeru koja dolazi u kontakt sa alergenima hrane i ima važnu ulogu u alergijskoj reakciji. Ranije se smatralo da epitelni sloj predstavlja samo fižičku barijeru koja sprečava prodor stranih supstanci u organizam. Danas je poznato da epitelna braijera ima centralnu ulogu u kontroli imunog odgovora, zahvaljujući sintezi citokina koji promovišu Th2 ćelijski odgovor i aktivaciju lokalnih dendritskih ćelija.
Aktivacija NF-κB transkripcionog faktora i njegova uloga u inflamatornom odgovoru su već prethodno proučavane. On igra ključnu ulogu u ćelijskom odgovoru na razne tipove stresnih situacija, kao što su bakterijske i virusne infekcije, inflamatorne stimuluse, slobodne radikale, dim od cigareta, karcinogene, endotoksine, citokine i mnogi drugi. Pri aktivaciji, NF-κB reguliše ekspresiju od skoro 400 gena, uključujući enzime (COX-2, iNOS), citokine (TNF, IL-1, IL-6, IL-8, hemokine), adhezione molekule, viralne proteine i angiogenetske faktore.
T1  - Aktivacija NF-kB signalnog puta u alergijskom odgovoru na glavni alergen kivija Act d 1
SP  - 2
EP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4016
ER  - 

@mastersthesis{
author = "Zlatanova, Milena",
year = "2020",
abstract = "Alergijske reakcije na hranu predstavljaju reakciju imunog sistema posredovanu IgE antitelima na proteine poreklom iz hrane. Epitelne ćelije gastrointestinalnog trakta predstavljaju prvu barijeru koja dolazi u kontakt sa alergenima hrane i ima važnu ulogu u alergijskoj reakciji. Ranije se smatralo da epitelni sloj predstavlja samo fižičku barijeru koja sprečava prodor stranih supstanci u organizam. Danas je poznato da epitelna braijera ima centralnu ulogu u kontroli imunog odgovora, zahvaljujući sintezi citokina koji promovišu Th2 ćelijski odgovor i aktivaciju lokalnih dendritskih ćelija.
Aktivacija NF-κB transkripcionog faktora i njegova uloga u inflamatornom odgovoru su već prethodno proučavane. On igra ključnu ulogu u ćelijskom odgovoru na razne tipove stresnih situacija, kao što su bakterijske i virusne infekcije, inflamatorne stimuluse, slobodne radikale, dim od cigareta, karcinogene, endotoksine, citokine i mnogi drugi. Pri aktivaciji, NF-κB reguliše ekspresiju od skoro 400 gena, uključujući enzime (COX-2, iNOS), citokine (TNF, IL-1, IL-6, IL-8, hemokine), adhezione molekule, viralne proteine i angiogenetske faktore.",
title = "Aktivacija NF-kB signalnog puta u alergijskom odgovoru na glavni alergen kivija Act d 1",
pages = "2-66",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4016"
}

Zlatanova, M.. (2020). Aktivacija NF-kB signalnog puta u alergijskom odgovoru na glavni alergen kivija Act d 1. , 2-66.
https://hdl.handle.net/21.15107/rcub_cherry_4016

Zlatanova M. Aktivacija NF-kB signalnog puta u alergijskom odgovoru na glavni alergen kivija Act d 1. 2020;:2-66.
https://hdl.handle.net/21.15107/rcub_cherry_4016 .

Zlatanova, Milena, "Aktivacija NF-kB signalnog puta u alergijskom odgovoru na glavni alergen kivija Act d 1" (2020):2-66,
https://hdl.handle.net/21.15107/rcub_cherry_4016 .

TY  - JOUR
AU  - Nešić, Andrijana N.
AU  - Čavić, Milena
AU  - Popović, Milica M.
AU  - Zlatanova, Milena
AU  - Pieters, Raymond
AU  - Smit, Joost
AU  - Gavrović-Jankulović, Marija
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3799
AB  - Impairment of the intestinal barrier is one of the key events in the initiation of the sensitization process in food allergy. The aim of this study was to explore the effects of kiwifruit allergen Act d 1 on intestinal permeability and tight junction protein (TJP) gene expression in vivo and to explore its potential to activate the NF-ĸB signaling pathway and to regulate expression of epithelial pro-allergenic cytokines. Influences of Act d 1 on TJP gene expression and pro-allergenic cytokines in the mouse intestine was analyzed by qPCR upon allergen administration by oral gavage. The effect on the in vivo intestinal permeability was assessed in ELISA by measuring the translocation of β-lactoglobulin (BLG) into circulation. The capacity of Act d 1 to activate the NF-ĸB pathway was tested in HEK293 cells by fluorescent microscopy and flow cytometry. Administration of Actinidin (Act d 1) increased intestinal permeability to the BLG. This was accompanied by changes in gene expression of TJP mRNA and pro-allergenic cytokines IL-25, IL-33, and thymic stromal lymphopoietin (TSLP) compared to the control. Act d 1 reduced TEER of the HEK293 monolayer, was positive in an NF-ĸB-reporter HEK293 cell assay, and induced secretion of TSLP. These findings shed more light on the molecular events in the sensitization process of kiwifruit but possibly also of other protease food allergens.
PB  - MDPI
T2  - Biomolecules
T1  - The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines
VL  - 9
IS  - 12
SP  - 816
DO  - 10.3390/biom9120816
ER  - 

@article{
author = "Nešić, Andrijana N. and Čavić, Milena and Popović, Milica M. and Zlatanova, Milena and Pieters, Raymond and Smit, Joost and Gavrović-Jankulović, Marija",
year = "2019",
abstract = "Impairment of the intestinal barrier is one of the key events in the initiation of the sensitization process in food allergy. The aim of this study was to explore the effects of kiwifruit allergen Act d 1 on intestinal permeability and tight junction protein (TJP) gene expression in vivo and to explore its potential to activate the NF-ĸB signaling pathway and to regulate expression of epithelial pro-allergenic cytokines. Influences of Act d 1 on TJP gene expression and pro-allergenic cytokines in the mouse intestine was analyzed by qPCR upon allergen administration by oral gavage. The effect on the in vivo intestinal permeability was assessed in ELISA by measuring the translocation of β-lactoglobulin (BLG) into circulation. The capacity of Act d 1 to activate the NF-ĸB pathway was tested in HEK293 cells by fluorescent microscopy and flow cytometry. Administration of Actinidin (Act d 1) increased intestinal permeability to the BLG. This was accompanied by changes in gene expression of TJP mRNA and pro-allergenic cytokines IL-25, IL-33, and thymic stromal lymphopoietin (TSLP) compared to the control. Act d 1 reduced TEER of the HEK293 monolayer, was positive in an NF-ĸB-reporter HEK293 cell assay, and induced secretion of TSLP. These findings shed more light on the molecular events in the sensitization process of kiwifruit but possibly also of other protease food allergens.",
publisher = "MDPI",
journal = "Biomolecules",
title = "The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines",
volume = "9",
number = "12",
pages = "816",
doi = "10.3390/biom9120816"
}

Nešić, A. N., Čavić, M., Popović, M. M., Zlatanova, M., Pieters, R., Smit, J.,& Gavrović-Jankulović, M.. (2019). The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines. in Biomolecules
MDPI., 9(12), 816.
https://doi.org/10.3390/biom9120816

Nešić AN, Čavić M, Popović MM, Zlatanova M, Pieters R, Smit J, Gavrović-Jankulović M. The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines. in Biomolecules. 2019;9(12):816.
doi:10.3390/biom9120816 .

Nešić, Andrijana N., Čavić, Milena, Popović, Milica M., Zlatanova, Milena, Pieters, Raymond, Smit, Joost, Gavrović-Jankulović, Marija, "The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines" in Biomolecules, 9, no. 12 (2019):816,
https://doi.org/10.3390/biom9120816 . .