TY  - JOUR
AU  - Bogdanović, Jelena
AU  - Milosavljević, Nenad
AU  - Vujčić, Zoran
AU  - Radotić, Ksenija
PY  - 2004
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/221
AB  - We studied the effect of nonionic detergent Tween 80 in extraction buffer on the activity and isoenzyme pattern of peroxidase from omorika needles. The activity of soluble peroxidases was studied using UV-VIS spectrophotometry and isoelectrofocusing. Soluble fraction obtained using extraction buffer with detergent had a higher activity and more isoenzymes in IEF pattern in comparison with the corresponding extract not including detergent in extraction medium.
AB  - Ispitivan je uticaj nejonskog detergenta Tween 80 u ekstrakcionom puferu na aktivnost i izoenzimski sastav peroksidaza u četinama omorike (Picea omorika (Pančić) Purkinye). Aktivnost unutarćelijske i vanćelijske slobodne peroksidaze ispitivana je korišćenjem UV-VIS spektrofotometrije i izoelektrofokusiranja. Ekstrakti dobijeni korišćenjem ekstrakcionog pufera sa detergentom Tween 80 imaju veću peroksidaznu aktivnost i veći broj izoenzima dobijenih izoelektrofokusiranjem u poređenju sa ekstraktima čiji ekstrakcioni pufer nije sadržao nejonski detergent.
T2  - Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta
T1  - Preparation of extracts from needles of omorika (Picea omorika (Pančić) Purkinye) for peroxidase activity and isoenzyme profile analyses
T1  - Priprema ekstrakta omorike za analizu aktivnosti peroksidaze i izoenzima
VL  - 40
IS  - 1-3
SP  - 159
EP  - 164
UR  - https://hdl.handle.net/21.15107/rcub_cherry_221
ER  - 

@article{
author = "Bogdanović, Jelena and Milosavljević, Nenad and Vujčić, Zoran and Radotić, Ksenija",
year = "2004",
abstract = "We studied the effect of nonionic detergent Tween 80 in extraction buffer on the activity and isoenzyme pattern of peroxidase from omorika needles. The activity of soluble peroxidases was studied using UV-VIS spectrophotometry and isoelectrofocusing. Soluble fraction obtained using extraction buffer with detergent had a higher activity and more isoenzymes in IEF pattern in comparison with the corresponding extract not including detergent in extraction medium., Ispitivan je uticaj nejonskog detergenta Tween 80 u ekstrakcionom puferu na aktivnost i izoenzimski sastav peroksidaza u četinama omorike (Picea omorika (Pančić) Purkinye). Aktivnost unutarćelijske i vanćelijske slobodne peroksidaze ispitivana je korišćenjem UV-VIS spektrofotometrije i izoelektrofokusiranja. Ekstrakti dobijeni korišćenjem ekstrakcionog pufera sa detergentom Tween 80 imaju veću peroksidaznu aktivnost i veći broj izoenzima dobijenih izoelektrofokusiranjem u poređenju sa ekstraktima čiji ekstrakcioni pufer nije sadržao nejonski detergent.",
journal = "Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta",
title = "Preparation of extracts from needles of omorika (Picea omorika (Pančić) Purkinye) for peroxidase activity and isoenzyme profile analyses, Priprema ekstrakta omorike za analizu aktivnosti peroksidaze i izoenzima",
volume = "40",
number = "1-3",
pages = "159-164",
url = "https://hdl.handle.net/21.15107/rcub_cherry_221"
}

Bogdanović, J., Milosavljević, N., Vujčić, Z.,& Radotić, K.. (2004). Preparation of extracts from needles of omorika (Picea omorika (Pančić) Purkinye) for peroxidase activity and isoenzyme profile analyses. in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta, 40(1-3), 159-164.
https://hdl.handle.net/21.15107/rcub_cherry_221

Bogdanović J, Milosavljević N, Vujčić Z, Radotić K. Preparation of extracts from needles of omorika (Picea omorika (Pančić) Purkinye) for peroxidase activity and isoenzyme profile analyses. in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta. 2004;40(1-3):159-164.
https://hdl.handle.net/21.15107/rcub_cherry_221 .

Bogdanović, Jelena, Milosavljević, Nenad, Vujčić, Zoran, Radotić, Ksenija, "Preparation of extracts from needles of omorika (Picea omorika (Pančić) Purkinye) for peroxidase activity and isoenzyme profile analyses" in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta, 40, no. 1-3 (2004):159-164,
https://hdl.handle.net/21.15107/rcub_cherry_221 .