TY  - CONF
AU  - Vidović, Marija
AU  - Battisti, Ilaria
AU  - Pantelić, Ana
AU  - Morina, Filis
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović Jovanović, Sonja
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5560
AB  - Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a long
period and fully resume metabolic functions upon rewatering in a very short period, even within
48 h. The goal of this study was to identify key candidates and pathways involved in desiccation tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics,
proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of the
cell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chain
were performed. In total, 1192 different protein groups were quantified by TMT-based comparative quantitative proteomics. Among them, 408 protein groups showed a statistically significant
difference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, the
majority of proteins related to photosynthetic processes were less abundant, while chlorophyll
fluorescence measurements implied shifting from linear photosynthetic transport (PET) to cyclic
electron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) were
reduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins (GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins),
were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wall
polymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/
HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific process orchestrated by several metabolic pathways and regulatory networks acting at the transcript, protein, metabolite and physiological levels.
C3  - 4th International Conference on Plant Biology, Book of Abstracts
T1  - Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects
SP  - 27
EP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5560
ER  - 

@conference{
author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Morina, Filis and Arrigoni, Giorgio and Masi, Antonio and Veljović Jovanović, Sonja",
year = "2022",
abstract = "Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a long
period and fully resume metabolic functions upon rewatering in a very short period, even within
48 h. The goal of this study was to identify key candidates and pathways involved in desiccation tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics,
proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of the
cell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chain
were performed. In total, 1192 different protein groups were quantified by TMT-based comparative quantitative proteomics. Among them, 408 protein groups showed a statistically significant
difference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, the
majority of proteins related to photosynthetic processes were less abundant, while chlorophyll
fluorescence measurements implied shifting from linear photosynthetic transport (PET) to cyclic
electron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) were
reduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins (GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins),
were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wall
polymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/
HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific process orchestrated by several metabolic pathways and regulatory networks acting at the transcript, protein, metabolite and physiological levels.",
journal = "4th International Conference on Plant Biology, Book of Abstracts",
title = "Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects",
pages = "27-27",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5560"
}

Vidović, M., Battisti, I., Pantelić, A., Morina, F., Arrigoni, G., Masi, A.,& Veljović Jovanović, S.. (2022). Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects. in 4th International Conference on Plant Biology, Book of Abstracts, 27-27.
https://hdl.handle.net/21.15107/rcub_cherry_5560

Vidović M, Battisti I, Pantelić A, Morina F, Arrigoni G, Masi A, Veljović Jovanović S. Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects. in 4th International Conference on Plant Biology, Book of Abstracts. 2022;:27-27.
https://hdl.handle.net/21.15107/rcub_cherry_5560 .

Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Morina, Filis, Arrigoni, Giorgio, Masi, Antonio, Veljović Jovanović, Sonja, "Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects" in 4th International Conference on Plant Biology, Book of Abstracts (2022):27-27,
https://hdl.handle.net/21.15107/rcub_cherry_5560 .

TY  - DATA
AU  - Pantelić, Ana
AU  - Stevanovic, Strahinja
AU  - Milic-Komic, Sonja
AU  - Kilibarda, Natasa
AU  - Vidović, Marija
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4882
AB  - Ramonda serbica de novo transcriptome database
PB  - MDPI
T2  - International Journal of Molecular Science
T1  - Ramonda serbica de novo transcriptome database related to the article: Pantelic, A.; Stevanović, S.; Milic-Komic, S.; Kilibarda, N.; Vidovic, M. Characterization and expression analysis of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves
VL  - n/a
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4882
ER  - 

@misc{
author = "Pantelić, Ana and Stevanovic, Strahinja and Milic-Komic, Sonja and Kilibarda, Natasa and Vidović, Marija",
year = "2022",
abstract = "Ramonda serbica de novo transcriptome database",
publisher = "MDPI",
journal = "International Journal of Molecular Science",
title = "Ramonda serbica de novo transcriptome database related to the article: Pantelic, A.; Stevanović, S.; Milic-Komic, S.; Kilibarda, N.; Vidovic, M. Characterization and expression analysis of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves",
volume = "n/a",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4882"
}

Pantelić, A., Stevanovic, S., Milic-Komic, S., Kilibarda, N.,& Vidović, M.. (2022). Ramonda serbica de novo transcriptome database related to the article: Pantelic, A.; Stevanović, S.; Milic-Komic, S.; Kilibarda, N.; Vidovic, M. Characterization and expression analysis of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves. in International Journal of Molecular Science
MDPI., n/a.
https://hdl.handle.net/21.15107/rcub_cherry_4882

Pantelić A, Stevanovic S, Milic-Komic S, Kilibarda N, Vidović M. Ramonda serbica de novo transcriptome database related to the article: Pantelic, A.; Stevanović, S.; Milic-Komic, S.; Kilibarda, N.; Vidovic, M. Characterization and expression analysis of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves. in International Journal of Molecular Science. 2022;n/a.
https://hdl.handle.net/21.15107/rcub_cherry_4882 .

Pantelić, Ana, Stevanovic, Strahinja, Milic-Komic, Sonja, Kilibarda, Natasa, Vidović, Marija, "Ramonda serbica de novo transcriptome database related to the article: Pantelic, A.; Stevanović, S.; Milic-Komic, S.; Kilibarda, N.; Vidovic, M. Characterization and expression analysis of the late embryogenesis abundant (LEA) proteins family in hydrated and desiccated Ramonda serbica Panc. leaves" in International Journal of Molecular Science, n/a (2022),
https://hdl.handle.net/21.15107/rcub_cherry_4882 .

TY  - CONF
AU  - Vidović, Marija
AU  - Stevanović, Strahinja
AU  - Pantelić, Ana
AU  - Veljović-Jovanović, Sonja
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4510
AB  - Introduction: Ramonda serbica Panc. is a resurrection plant that can survive a long period of severe dehydrationdesiccation.
Desiccation induces cellular membrane integrity loss, protein aggregation, and denaturation, as well as
accelerated generation of reactive oxygen species. However, R. serbica can fully recover its metabolic functions
already one day upon watering [1].
Aim: to obtain more insight into the mechanisms of desiccation tolerance in R. serbica by differential de novo
transcriptomics of hydrated (HL) and desiccated leaves (DL).
C3  - Belgrade BioInformatics Conference 2021, 21-25 June, Vinča, Serbia
T1  - De Novo Transcriptome Sequencing of Ramonda serbica: Identification of the Candidate Genes Involved in the Desiccation Tolerance
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4510
ER  - 

@conference{
author = "Vidović, Marija and Stevanović, Strahinja and Pantelić, Ana and Veljović-Jovanović, Sonja",
year = "2021",
abstract = "Introduction: Ramonda serbica Panc. is a resurrection plant that can survive a long period of severe dehydrationdesiccation.
Desiccation induces cellular membrane integrity loss, protein aggregation, and denaturation, as well as
accelerated generation of reactive oxygen species. However, R. serbica can fully recover its metabolic functions
already one day upon watering [1].
Aim: to obtain more insight into the mechanisms of desiccation tolerance in R. serbica by differential de novo
transcriptomics of hydrated (HL) and desiccated leaves (DL).",
journal = "Belgrade BioInformatics Conference 2021, 21-25 June, Vinča, Serbia",
title = "De Novo Transcriptome Sequencing of Ramonda serbica: Identification of the Candidate Genes Involved in the Desiccation Tolerance",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4510"
}

Vidović, M., Stevanović, S., Pantelić, A.,& Veljović-Jovanović, S.. (2021). De Novo Transcriptome Sequencing of Ramonda serbica: Identification of the Candidate Genes Involved in the Desiccation Tolerance. in Belgrade BioInformatics Conference 2021, 21-25 June, Vinča, Serbia.
https://hdl.handle.net/21.15107/rcub_cherry_4510

Vidović M, Stevanović S, Pantelić A, Veljović-Jovanović S. De Novo Transcriptome Sequencing of Ramonda serbica: Identification of the Candidate Genes Involved in the Desiccation Tolerance. in Belgrade BioInformatics Conference 2021, 21-25 June, Vinča, Serbia. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4510 .

Vidović, Marija, Stevanović, Strahinja, Pantelić, Ana, Veljović-Jovanović, Sonja, "De Novo Transcriptome Sequencing of Ramonda serbica: Identification of the Candidate Genes Involved in the Desiccation Tolerance" in Belgrade BioInformatics Conference 2021, 21-25 June, Vinča, Serbia (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4510 .

TY  - CONF
AU  - Pantelić, Ana
AU  - Stevanović, Strahinja
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4503
UR  - http://ojs.pmf.uns.ac.rs/index.php/dbe_serbica/index
AB  - An extreme loss of cellular water or desiccation (5-10% of relative water content) leads to protein denaturation, aggregation and degradation, and affects the fluidity of membrane lipids resulting in loss of membrane integrity [1]. The essential constituents of vegetative desiccation tolerance in so-called resurrection plants are late embryogenesis abundant proteins (LEAPs). This heterogeneous group of anhydrobiosis-related intrinsically disordered proteins forms mostly random conformation when fully hydrated, turning into compact α-helices during desiccation [2]. Based on in vitro studies, LEAPs can be involved in water binding, ion sequestration, stabilization of both membrane and enzymes during freezing or drying, while by forming intracellular proteinaceous condensates they increase structural integrity and intracellular viscosity of cells during desiccation.
Here, we identify 164 members of LEA gene family in endemic and relict resurrection species Ramonda serbica by integrating previously done de novo transcriptome and homologues protein motifs. Identified LEAPs were classification into six groups according to Protein family (PFAM) database and the most populated group was LEA4 containing 47% of total identified LEAPs. By using four secondary structure predictors, we showed that this group exhibited a high propensity to form amphipathic α-helices (81% of total sequence length is predicted to form α-helical structure). This implies that charged residues might be exposed to the solvent, while hydrophobic amino acids might interact with lipid bilayers or with other target proteins in the cell. In addition, as predicted by several bioinformatic tools, more than 70% of identified LEAPs were found to be highly disordered (~64%). Structural characterization of LEAPs is a key to understand their function and regulation of their intrinsic structural disorder-to-order transition during desiccation. These findings will promote transformative advancements in various fields, such as the development of new strategies in neurodegenerative disorders, cell preservation technology and the improvement of crop drought tolerance.
PB  - Novi Sad : Faculty of Sciences, Department of Biology and Ecology
C3  - Biologia Serbica
T1  - De Novo Transcriptome Sequencing of Ramonda serbica: Identification of Late Embryogenesis Abundant Proteins
VL  - 43
IS  - 1 (spec. ed.)
SP  - 65
EP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4503
ER  - 

@conference{
author = "Pantelić, Ana and Stevanović, Strahinja and Kilibarda, Nataša and Vidović, Marija",
year = "2021",
abstract = "An extreme loss of cellular water or desiccation (5-10% of relative water content) leads to protein denaturation, aggregation and degradation, and affects the fluidity of membrane lipids resulting in loss of membrane integrity [1]. The essential constituents of vegetative desiccation tolerance in so-called resurrection plants are late embryogenesis abundant proteins (LEAPs). This heterogeneous group of anhydrobiosis-related intrinsically disordered proteins forms mostly random conformation when fully hydrated, turning into compact α-helices during desiccation [2]. Based on in vitro studies, LEAPs can be involved in water binding, ion sequestration, stabilization of both membrane and enzymes during freezing or drying, while by forming intracellular proteinaceous condensates they increase structural integrity and intracellular viscosity of cells during desiccation.
Here, we identify 164 members of LEA gene family in endemic and relict resurrection species Ramonda serbica by integrating previously done de novo transcriptome and homologues protein motifs. Identified LEAPs were classification into six groups according to Protein family (PFAM) database and the most populated group was LEA4 containing 47% of total identified LEAPs. By using four secondary structure predictors, we showed that this group exhibited a high propensity to form amphipathic α-helices (81% of total sequence length is predicted to form α-helical structure). This implies that charged residues might be exposed to the solvent, while hydrophobic amino acids might interact with lipid bilayers or with other target proteins in the cell. In addition, as predicted by several bioinformatic tools, more than 70% of identified LEAPs were found to be highly disordered (~64%). Structural characterization of LEAPs is a key to understand their function and regulation of their intrinsic structural disorder-to-order transition during desiccation. These findings will promote transformative advancements in various fields, such as the development of new strategies in neurodegenerative disorders, cell preservation technology and the improvement of crop drought tolerance.",
publisher = "Novi Sad : Faculty of Sciences, Department of Biology and Ecology",
journal = "Biologia Serbica",
title = "De Novo Transcriptome Sequencing of Ramonda serbica: Identification of Late Embryogenesis Abundant Proteins",
volume = "43",
number = "1 (spec. ed.)",
pages = "65-65",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4503"
}

Pantelić, A., Stevanović, S., Kilibarda, N.,& Vidović, M.. (2021). De Novo Transcriptome Sequencing of Ramonda serbica: Identification of Late Embryogenesis Abundant Proteins. in Biologia Serbica
Novi Sad : Faculty of Sciences, Department of Biology and Ecology., 43(1 (spec. ed.)), 65-65.
https://hdl.handle.net/21.15107/rcub_cherry_4503

Pantelić A, Stevanović S, Kilibarda N, Vidović M. De Novo Transcriptome Sequencing of Ramonda serbica: Identification of Late Embryogenesis Abundant Proteins. in Biologia Serbica. 2021;43(1 (spec. ed.)):65-65.
https://hdl.handle.net/21.15107/rcub_cherry_4503 .

Pantelić, Ana, Stevanović, Strahinja, Kilibarda, Nataša, Vidović, Marija, "De Novo Transcriptome Sequencing of Ramonda serbica: Identification of Late Embryogenesis Abundant Proteins" in Biologia Serbica, 43, no. 1 (spec. ed.) (2021):65-65,
https://hdl.handle.net/21.15107/rcub_cherry_4503 .

TY  - THES
AU  - Pantelić, Ana
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4602
AB  - Cilj ove master teze je optimizacija procesa dobijanja prečišćenog proteina zastupljenog u kasnoj fazi embriogeneze (LEAP) iz biljke vaskrsnice Ramonda serbica tehnologijom rekombinantne DNK. Ova procedura obezbeđuje značajne količine LEA proteina radi daljih strukturnih i funkcionalnih analiza, prevashodno za ispitivanje potencijalnih interakcija sa rekombinantnim humanim α-sinukelinom, dobijenog u mom Završnom radu. Detalji vezani za opis tehnika rekombinantne DNK tehnologije i potencijalnu problematiku su opisani u pomenutom Završnom radu.
T1  - Optimizacija protokola proizvodnje proteina zastupljenog u kasnoj fazi embriogeneze (RsLEA_30) iz biljke vaskrsnice Ramonda serbica tehnikom rekombinantne DNK u bakteriji Escherichia coli
SP  - 2
EP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4602
ER  - 

@mastersthesis{
author = "Pantelić, Ana",
year = "2021",
abstract = "Cilj ove master teze je optimizacija procesa dobijanja prečišćenog proteina zastupljenog u kasnoj fazi embriogeneze (LEAP) iz biljke vaskrsnice Ramonda serbica tehnologijom rekombinantne DNK. Ova procedura obezbeđuje značajne količine LEA proteina radi daljih strukturnih i funkcionalnih analiza, prevashodno za ispitivanje potencijalnih interakcija sa rekombinantnim humanim α-sinukelinom, dobijenog u mom Završnom radu. Detalji vezani za opis tehnika rekombinantne DNK tehnologije i potencijalnu problematiku su opisani u pomenutom Završnom radu.",
title = "Optimizacija protokola proizvodnje proteina zastupljenog u kasnoj fazi embriogeneze (RsLEA_30) iz biljke vaskrsnice Ramonda serbica tehnikom rekombinantne DNK u bakteriji Escherichia coli",
pages = "2-94",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4602"
}

Pantelić, A.. (2021). Optimizacija protokola proizvodnje proteina zastupljenog u kasnoj fazi embriogeneze (RsLEA_30) iz biljke vaskrsnice Ramonda serbica tehnikom rekombinantne DNK u bakteriji Escherichia coli. , 2-94.
https://hdl.handle.net/21.15107/rcub_cherry_4602

Pantelić A. Optimizacija protokola proizvodnje proteina zastupljenog u kasnoj fazi embriogeneze (RsLEA_30) iz biljke vaskrsnice Ramonda serbica tehnikom rekombinantne DNK u bakteriji Escherichia coli. 2021;:2-94.
https://hdl.handle.net/21.15107/rcub_cherry_4602 .

Pantelić, Ana, "Optimizacija protokola proizvodnje proteina zastupljenog u kasnoj fazi embriogeneze (RsLEA_30) iz biljke vaskrsnice Ramonda serbica tehnikom rekombinantne DNK u bakteriji Escherichia coli" (2021):2-94,
https://hdl.handle.net/21.15107/rcub_cherry_4602 .

TY  - GEN
AU  - Pantelić, Ana
AU  - Stevanović, Strahinja
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4506
PB  - Belgrade BioInformatics
T2  - Belgrade BioInformatics Conference 2021
T1  - De Novo Transcriptome Sequencing of Ramonda serbica : Identification of Late Embryogenesis Abundant Proteins
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4506
ER  - 

@misc{
author = "Pantelić, Ana and Stevanović, Strahinja and Kilibarda, Nataša and Vidović, Marija",
year = "2021",
publisher = "Belgrade BioInformatics",
journal = "Belgrade BioInformatics Conference 2021",
title = "De Novo Transcriptome Sequencing of Ramonda serbica : Identification of Late Embryogenesis Abundant Proteins",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4506"
}

Pantelić, A., Stevanović, S., Kilibarda, N.,& Vidović, M.. (2021). De Novo Transcriptome Sequencing of Ramonda serbica : Identification of Late Embryogenesis Abundant Proteins. in Belgrade BioInformatics Conference 2021
Belgrade BioInformatics..
https://hdl.handle.net/21.15107/rcub_cherry_4506

Pantelić A, Stevanović S, Kilibarda N, Vidović M. De Novo Transcriptome Sequencing of Ramonda serbica : Identification of Late Embryogenesis Abundant Proteins. in Belgrade BioInformatics Conference 2021. 2021;.
https://hdl.handle.net/21.15107/rcub_cherry_4506 .

Pantelić, Ana, Stevanović, Strahinja, Kilibarda, Nataša, Vidović, Marija, "De Novo Transcriptome Sequencing of Ramonda serbica : Identification of Late Embryogenesis Abundant Proteins" in Belgrade BioInformatics Conference 2021 (2021),
https://hdl.handle.net/21.15107/rcub_cherry_4506 .

TY  - THES
AU  - Pantelić, Ana
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4145
AB  - U ovom radu optimizovani su uslovi proizvodnje α-sinukleina u fuziji sa fluorescentnim mCerulean3 proteinom. Za ekspresiju proteina u E. coli su korišćena dva medijuma (LB sa glicerolom kao izvorom ugljenika i autoindukcioni medijum). Može se očekivati da se iz ćelija gajenih na LB medijumu prečisti >20 mg/L proteina, dok autoindukcioni medijum, iako daje nižu koncentraciju proteina u ćeliji, daje veću količinu biomase, pa se može očekivati čak i preko 100 mg/L proteina iz bakterija gajenih u ovom medijumu. Takođe, nakon jednog prečišćavanja na IMAC dobijeni protein je bio oko 70% čistoće.
U ovom radu kao metoda za liziranje korišćena je sonikacija uz odmrzavanje i zamrzavanje. Međutim, najverovatnije da ćelije nisu bile u potpunosti lizirane, pa bi verovatno za dalje izolovanje bilo više puta ponoviti ultrazvučni tretman uz zamrzavanja i odmrzavanje. S obzirom na prisustvo His taga u ovom konstruktu, prečišćavanje IMAC-om je metoda prvog izbora koja obezbeđuje visok stepen prečišćenosti u jednom koraku. Potrebno bi bilo optimizovati uslove prečišćavanja, npr. promenom pufera za ispiranje nevezanih proteina ili promenom metalnog jona (npr. korišćenje Co2+) vezanog za helirajući matriks.
Iako postoji mogućnost oligomerizacije α-sinukleina u fuziji sa fluorescentnim mCerulean3 proteinom, do nje, pod uslovima u kojima je ovde eksprimiran protein, ne dolazi. S obzirom da fuzioni partner mCerulean3 fluorescira, vizuelizacija i praćenje hromatografskih koraka su jednostavni za izvođenje.
T1  - Optimizacija uslova ekspresije humanog α-sinukleina fuzonisanog sa mCerulean3 fluorescentnim proteinom u bakteriji Escherichia coli
SP  - 2
EP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_cherry_4145
ER  - 

@misc{
author = "Pantelić, Ana",
year = "2020",
abstract = "U ovom radu optimizovani su uslovi proizvodnje α-sinukleina u fuziji sa fluorescentnim mCerulean3 proteinom. Za ekspresiju proteina u E. coli su korišćena dva medijuma (LB sa glicerolom kao izvorom ugljenika i autoindukcioni medijum). Može se očekivati da se iz ćelija gajenih na LB medijumu prečisti >20 mg/L proteina, dok autoindukcioni medijum, iako daje nižu koncentraciju proteina u ćeliji, daje veću količinu biomase, pa se može očekivati čak i preko 100 mg/L proteina iz bakterija gajenih u ovom medijumu. Takođe, nakon jednog prečišćavanja na IMAC dobijeni protein je bio oko 70% čistoće.
U ovom radu kao metoda za liziranje korišćena je sonikacija uz odmrzavanje i zamrzavanje. Međutim, najverovatnije da ćelije nisu bile u potpunosti lizirane, pa bi verovatno za dalje izolovanje bilo više puta ponoviti ultrazvučni tretman uz zamrzavanja i odmrzavanje. S obzirom na prisustvo His taga u ovom konstruktu, prečišćavanje IMAC-om je metoda prvog izbora koja obezbeđuje visok stepen prečišćenosti u jednom koraku. Potrebno bi bilo optimizovati uslove prečišćavanja, npr. promenom pufera za ispiranje nevezanih proteina ili promenom metalnog jona (npr. korišćenje Co2+) vezanog za helirajući matriks.
Iako postoji mogućnost oligomerizacije α-sinukleina u fuziji sa fluorescentnim mCerulean3 proteinom, do nje, pod uslovima u kojima je ovde eksprimiran protein, ne dolazi. S obzirom da fuzioni partner mCerulean3 fluorescira, vizuelizacija i praćenje hromatografskih koraka su jednostavni za izvođenje.",
title = "Optimizacija uslova ekspresije humanog α-sinukleina fuzonisanog sa mCerulean3 fluorescentnim proteinom u bakteriji Escherichia coli",
pages = "2-53",
url = "https://hdl.handle.net/21.15107/rcub_cherry_4145"
}

Pantelić, A.. (2020). Optimizacija uslova ekspresije humanog α-sinukleina fuzonisanog sa mCerulean3 fluorescentnim proteinom u bakteriji Escherichia coli. , 2-53.
https://hdl.handle.net/21.15107/rcub_cherry_4145

Pantelić A. Optimizacija uslova ekspresije humanog α-sinukleina fuzonisanog sa mCerulean3 fluorescentnim proteinom u bakteriji Escherichia coli. 2020;:2-53.
https://hdl.handle.net/21.15107/rcub_cherry_4145 .

Pantelić, Ana, "Optimizacija uslova ekspresije humanog α-sinukleina fuzonisanog sa mCerulean3 fluorescentnim proteinom u bakteriji Escherichia coli" (2020):2-53,
https://hdl.handle.net/21.15107/rcub_cherry_4145 .

TY  - JOUR
AU  - Relić, Dubravka
AU  - Đorđević, Dragana S.
AU  - Sakan, Sanja M.
AU  - Anđelković, Ivan
AU  - Pantelić, Ana
AU  - Stanković, Ratomir
AU  - Popović, Aleksandar R.
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1380
AB  - In this paper, the main objective was fractionation of Cd, Cu, Ni, Pb, Zn, Ca, Fe, and K in certificate material and sediment samples gathered from and around the Petrochemical Industry using the conventional, microwave and ultrasonic sequential extraction. Microwave oven and ultrasound bath were used as an energy source for achieving faster extraction. Additional heating and boiling of samples were avoided by using lower power and shorter time for microwave and ultrasound extraction. Precision and accuracy of procedure were evaluated by using certificate material (BCR701). Acceptable accuracy of metals (87.0-111.3 %) was achieved for all three-step sequential of conventional extraction protocol. An accuracy of the fourth step has been verified with two certificate materials: BCR143R and 146R. The range of total extracted metal concentrations from sediments was similar for all three extraction techniques. A significant high percentage of Cd, Cu, and Zn were obtained after extraction of the exchangeable and acid soluble sediment fraction. Principal component analysis of values obtained after determination of risk assessment code using conventional and ultrasound sequential extraction show similarity of these values. Accuracy, recovery, and risk assessment code values imply that ultrasound sequential extraction is a more suitable, accelerated sequential extraction procedure (30 min per extraction step) than microwave extraction in applied conditions.
PB  - Springer, Dordrecht
T2  - Environmental Monitoring and Assessment
T1  - Conventional, microwave, and ultrasound sequential extractions for the fractionation of metals in sediments within the Petrochemical Industry, Serbia
VL  - 185
IS  - 9
SP  - 7627
EP  - 7645
DO  - 10.1007/s10661-013-3124-4
ER  - 

@article{
author = "Relić, Dubravka and Đorđević, Dragana S. and Sakan, Sanja M. and Anđelković, Ivan and Pantelić, Ana and Stanković, Ratomir and Popović, Aleksandar R.",
year = "2013",
abstract = "In this paper, the main objective was fractionation of Cd, Cu, Ni, Pb, Zn, Ca, Fe, and K in certificate material and sediment samples gathered from and around the Petrochemical Industry using the conventional, microwave and ultrasonic sequential extraction. Microwave oven and ultrasound bath were used as an energy source for achieving faster extraction. Additional heating and boiling of samples were avoided by using lower power and shorter time for microwave and ultrasound extraction. Precision and accuracy of procedure were evaluated by using certificate material (BCR701). Acceptable accuracy of metals (87.0-111.3 %) was achieved for all three-step sequential of conventional extraction protocol. An accuracy of the fourth step has been verified with two certificate materials: BCR143R and 146R. The range of total extracted metal concentrations from sediments was similar for all three extraction techniques. A significant high percentage of Cd, Cu, and Zn were obtained after extraction of the exchangeable and acid soluble sediment fraction. Principal component analysis of values obtained after determination of risk assessment code using conventional and ultrasound sequential extraction show similarity of these values. Accuracy, recovery, and risk assessment code values imply that ultrasound sequential extraction is a more suitable, accelerated sequential extraction procedure (30 min per extraction step) than microwave extraction in applied conditions.",
publisher = "Springer, Dordrecht",
journal = "Environmental Monitoring and Assessment",
title = "Conventional, microwave, and ultrasound sequential extractions for the fractionation of metals in sediments within the Petrochemical Industry, Serbia",
volume = "185",
number = "9",
pages = "7627-7645",
doi = "10.1007/s10661-013-3124-4"
}

Relić, D., Đorđević, D. S., Sakan, S. M., Anđelković, I., Pantelić, A., Stanković, R.,& Popović, A. R.. (2013). Conventional, microwave, and ultrasound sequential extractions for the fractionation of metals in sediments within the Petrochemical Industry, Serbia. in Environmental Monitoring and Assessment
Springer, Dordrecht., 185(9), 7627-7645.
https://doi.org/10.1007/s10661-013-3124-4

Relić D, Đorđević DS, Sakan SM, Anđelković I, Pantelić A, Stanković R, Popović AR. Conventional, microwave, and ultrasound sequential extractions for the fractionation of metals in sediments within the Petrochemical Industry, Serbia. in Environmental Monitoring and Assessment. 2013;185(9):7627-7645.
doi:10.1007/s10661-013-3124-4 .

Relić, Dubravka, Đorđević, Dragana S., Sakan, Sanja M., Anđelković, Ivan, Pantelić, Ana, Stanković, Ratomir, Popović, Aleksandar R., "Conventional, microwave, and ultrasound sequential extractions for the fractionation of metals in sediments within the Petrochemical Industry, Serbia" in Environmental Monitoring and Assessment, 185, no. 9 (2013):7627-7645,
https://doi.org/10.1007/s10661-013-3124-4 . .