TY  - CONF
AU  - Lazović, Mila Č.
AU  - Jović, Marko D.
AU  - Dimkić, Ivica Z.
AU  - Milojković-Opsenica, Dušanka
AU  - Ristivojević, Petar
AU  - Trifković, Jelena 
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6220
AB  - Natural Deep Eutectic Solvents (NADES) are novel and promising solvents for green extraction of phytochemicals from food and agricultural products. NADES are made of natural origin compounds that are connected via hydrogen bond and have unique tuneable properties1. Numerous studies investigated the extraction of bioactive compounds using NADES resulted in better extraction efficiency compared to conventional solvents, suggesting large potential of these solvents2,3. Low-toxicity, natural origin and affordability of NADES-based extracts enables their application as a preservative in the food industry or functional ingredient of food supplements. The aim of this study was to develop an efficient eco-friendly method for the extraction of phenolic compounds from berries, blueberry, chokeberry and black goji berry, using NADES. This goal will be achieved through investigation of extraction efficiency of applied NADES based on three different approaches, a) chromatographic, through high-performance thin layer chromatography (HPTLC) fingerprint analysis, ultra-high-performance liquid chromatography with a diode array detector and a triple-quadrupole mass spectrometer (UHPLC-DAD-MS/MS) target analysis and bioautography, b) spectroscopic, through quality control parameters, total phenolic content (TPC), total flavonoid content (TFC) and radical scavenging activity (RSA), and c) microbiological, through well diffusion method and minimum inhibitory concentration (MIC).
In this study, 36 NADES mixtures, prepared from primary plant metabolites, were tested as green alternatives for extraction of phenolics from three berries. Different hydrogen-bond acceptors (HBA), such as choline chloride, L-proline, L-glycine, and L-lysine were mixed with various hydrogen-bond donors (HBD), four organic acids, two sugars, glycerol and urea, at various molar ratios for NADES preparation. Guided by the fact that extraction efficiency depends on polarity, viscosity and dissolving ability of solvents4, the influence of HBA, HBD and water content were investigated. Methanol, as conventional solvent, was used as contrastive solvent.
Choline chloride-based NADES in combination with malic acid, glycerol or urea showed as most efficient. All groups of studied NADES showed various extraction capabilities for phenolic compounds based on chromatographic evaluation and spectrophotometric tests. These results indicate great power of NADES solvents towards selective extraction of phenolics and shows that NADES are designer solvents. Appropriate selection of components for NADES preparation can result in selective extraction of specific phenolic compounds, regardless of how similar structures of targeted compounds are or how complex the matrix is, which gives a significant advantage for application of NADES.
C3  - XXII Congress EuroFoodChem, Book of abstracts
T1  - Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6220
ER  - 

@conference{
author = "Lazović, Mila Č. and Jović, Marko D. and Dimkić, Ivica Z. and Milojković-Opsenica, Dušanka and Ristivojević, Petar and Trifković, Jelena ",
year = "2023",
abstract = "Natural Deep Eutectic Solvents (NADES) are novel and promising solvents for green extraction of phytochemicals from food and agricultural products. NADES are made of natural origin compounds that are connected via hydrogen bond and have unique tuneable properties1. Numerous studies investigated the extraction of bioactive compounds using NADES resulted in better extraction efficiency compared to conventional solvents, suggesting large potential of these solvents2,3. Low-toxicity, natural origin and affordability of NADES-based extracts enables their application as a preservative in the food industry or functional ingredient of food supplements. The aim of this study was to develop an efficient eco-friendly method for the extraction of phenolic compounds from berries, blueberry, chokeberry and black goji berry, using NADES. This goal will be achieved through investigation of extraction efficiency of applied NADES based on three different approaches, a) chromatographic, through high-performance thin layer chromatography (HPTLC) fingerprint analysis, ultra-high-performance liquid chromatography with a diode array detector and a triple-quadrupole mass spectrometer (UHPLC-DAD-MS/MS) target analysis and bioautography, b) spectroscopic, through quality control parameters, total phenolic content (TPC), total flavonoid content (TFC) and radical scavenging activity (RSA), and c) microbiological, through well diffusion method and minimum inhibitory concentration (MIC).
In this study, 36 NADES mixtures, prepared from primary plant metabolites, were tested as green alternatives for extraction of phenolics from three berries. Different hydrogen-bond acceptors (HBA), such as choline chloride, L-proline, L-glycine, and L-lysine were mixed with various hydrogen-bond donors (HBD), four organic acids, two sugars, glycerol and urea, at various molar ratios for NADES preparation. Guided by the fact that extraction efficiency depends on polarity, viscosity and dissolving ability of solvents4, the influence of HBA, HBD and water content were investigated. Methanol, as conventional solvent, was used as contrastive solvent.
Choline chloride-based NADES in combination with malic acid, glycerol or urea showed as most efficient. All groups of studied NADES showed various extraction capabilities for phenolic compounds based on chromatographic evaluation and spectrophotometric tests. These results indicate great power of NADES solvents towards selective extraction of phenolics and shows that NADES are designer solvents. Appropriate selection of components for NADES preparation can result in selective extraction of specific phenolic compounds, regardless of how similar structures of targeted compounds are or how complex the matrix is, which gives a significant advantage for application of NADES.",
journal = "XXII Congress EuroFoodChem, Book of abstracts",
title = "Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6220"
}

Lazović, M. Č., Jović, M. D., Dimkić, I. Z., Milojković-Opsenica, D., Ristivojević, P.,& Trifković, J.. (2023). Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries. in XXII Congress EuroFoodChem, Book of abstracts.
https://hdl.handle.net/21.15107/rcub_cherry_6220

Lazović MČ, Jović MD, Dimkić IZ, Milojković-Opsenica D, Ristivojević P, Trifković J. Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries. in XXII Congress EuroFoodChem, Book of abstracts. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6220 .

Lazović, Mila Č., Jović, Marko D., Dimkić, Ivica Z., Milojković-Opsenica, Dušanka, Ristivojević, Petar, Trifković, Jelena , "Potential application of green extracts rich in phenolics for innovative functional foods: Natural deep eutectic solvents as medium for isolation of biocompounds from berries" in XXII Congress EuroFoodChem, Book of abstracts (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6220 .

TY  - CONF
AU  - Lazović, Mila Č.
AU  - Krstić, Đurđa D.
AU  - Jović, Marko D.
AU  - Trifković, Jelena 
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6217
AB  - Blackberry wine is produced by a yeast fermentation of natural saccharides present in blackberry juice. This wine is traditionally consumed in Balkans, mostly as a dessert wine, but in recent years it is gaining more attention due to unique flavour and potential health benefits. Blackberries are a natural source of many minerals and bioactive phytochemicals such as vitamins, folic acid, anthocyanins, and phenolics1,2. Considering biological potential of blackberries and their products aim of this study was to evaluate quality of Serbian blackberry wines. This quality assessment was achieved through determination of: (a) the phenolic profile by high-performance thin layer chromatography (HPTLC); (b) total phenolic (TPC) and anthocyanin content (TAC); (c) in vitro antioxidant activity by DPPH and ABTS radical scavenging assays and total antioxidant capacity (d) in vitro antimicrobial activity by the agar diffusion test; and (e) the content of macro, micro and toxic elements by inductively coupled plasma mass spectrometry (ICP-MS). Twelve blackberry wines from different geographical regions were tested indicating various phenolic profiles and great antioxidant and antimicrobial activity. Strong positive correlation was confirmed between TPC and antioxidant activity determined by DPPH and ABTS assays. Additionally, high levels of antibacterial activity were shown against all six tested strains, but primarily against Staphylococcus aureus and Micrococcus lysodeikticus. Samples were rich in macroelements, such as Ca, K, Mg, Na, P and Zn, and microelements Cu and Fe, while Mn was present in higher concentration only in one sample. Toxic metals Cd and Pb were present in low concertation in all samples. These results indicate great quality of Serbian blackberry wines concerning minerals, phenolics, antioxidant and antimicrobial activity, which support its health potential.
C3  - 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science
T1  - Phenolic and multi-elemental profiles as a tool for quality assessment of Serbian blackberry wines
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6217
ER  - 

@conference{
author = "Lazović, Mila Č. and Krstić, Đurđa D. and Jović, Marko D. and Trifković, Jelena ",
year = "2023",
abstract = "Blackberry wine is produced by a yeast fermentation of natural saccharides present in blackberry juice. This wine is traditionally consumed in Balkans, mostly as a dessert wine, but in recent years it is gaining more attention due to unique flavour and potential health benefits. Blackberries are a natural source of many minerals and bioactive phytochemicals such as vitamins, folic acid, anthocyanins, and phenolics1,2. Considering biological potential of blackberries and their products aim of this study was to evaluate quality of Serbian blackberry wines. This quality assessment was achieved through determination of: (a) the phenolic profile by high-performance thin layer chromatography (HPTLC); (b) total phenolic (TPC) and anthocyanin content (TAC); (c) in vitro antioxidant activity by DPPH and ABTS radical scavenging assays and total antioxidant capacity (d) in vitro antimicrobial activity by the agar diffusion test; and (e) the content of macro, micro and toxic elements by inductively coupled plasma mass spectrometry (ICP-MS). Twelve blackberry wines from different geographical regions were tested indicating various phenolic profiles and great antioxidant and antimicrobial activity. Strong positive correlation was confirmed between TPC and antioxidant activity determined by DPPH and ABTS assays. Additionally, high levels of antibacterial activity were shown against all six tested strains, but primarily against Staphylococcus aureus and Micrococcus lysodeikticus. Samples were rich in macroelements, such as Ca, K, Mg, Na, P and Zn, and microelements Cu and Fe, while Mn was present in higher concentration only in one sample. Toxic metals Cd and Pb were present in low concertation in all samples. These results indicate great quality of Serbian blackberry wines concerning minerals, phenolics, antioxidant and antimicrobial activity, which support its health potential.",
journal = "9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science",
title = "Phenolic and multi-elemental profiles as a tool for quality assessment of Serbian blackberry wines",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6217"
}

Lazović, M. Č., Krstić, Đ. D., Jović, M. D.,& Trifković, J.. (2023). Phenolic and multi-elemental profiles as a tool for quality assessment of Serbian blackberry wines. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science.
https://hdl.handle.net/21.15107/rcub_cherry_6217

Lazović MČ, Krstić ĐD, Jović MD, Trifković J. Phenolic and multi-elemental profiles as a tool for quality assessment of Serbian blackberry wines. in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science. 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6217 .

Lazović, Mila Č., Krstić, Đurđa D., Jović, Marko D., Trifković, Jelena , "Phenolic and multi-elemental profiles as a tool for quality assessment of Serbian blackberry wines" in 9th Conference of Young Chemists of Serbia, Book of Abstracts, 4th November 2023, University of Novi Sad - Faculty of Science (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6217 .

TY  - CONF
AU  - Lazović, Mila Č.
AU  - Jankov, Milica S.
AU  - Trifković, Jelena 
AU  - Cvijetić, Ilija 
AU  - Ristivojević, Petar
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6215
AB  - One of the main principles of green analytical chemistry is to eliminate or reduce the use of organic solvents and reagents, and therefore use of Natural Deep Eutectic Solvents (NADES) as medium in sample extraction was recognized as green alternative to conventional one. In this paper, 19 different natural deep eutectic solvents were used for the extraction of phenolic compounds from Teucrium chamaedrys L. Chemical profiles of the extracts were obtained by applying high-performance thin-layer chromatography (HPTLC), which is a green technique due to the simultaneous analysis of a large number of samples, using small amounts of extracts and minimal amounts of solvents. Greenness of proposed methodology was estimated by NEMI pictogram and analytical eco-scale.
AB  - Jedan od glavnih ciljeva zelene analitičke hemije je eliminisanje ili smanjenje upotrebe organskih rastvarača i reagenasa i zbog toga je primena prirodnih eutektičkih smeša (NADES - natural deep eutectic solvents) kao medijuma za ekstrakciju uzoraka prepoznata kao zelena alternativa. U ovom radu je primenjeno 19 različitih prirodnih eutektičkih smeša za ekstrakciju fenolnih jedinjenja iz biljke podubice. Za hemijsko profilisanje ekstrakata korišćena je visoko-efikasna tankoslojna hromatografija (HPTLC – High-performance thin-layer chromatography) koja predstavlja zelenu tehniku zbog istovremene analize velikog broja uzoraka, uz upotrebu male količine ekstrakata i minimalnih količina rastvarača. Ekološka prihvatljivost predložene metodologije procenjena je primenom Nacionalnog indeksa uticaja metode na životnu sredinu i eko-skale.
C3  - 58. Savetovanje Srpskog hemijskog društva - Knjiga radova
T1  - Green analytical approach for extraction and chemical profiling of phenolic compounds from natural products: A case study of Teucrium chamaedrys L.
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6215
ER  - 

@conference{
author = "Lazović, Mila Č. and Jankov, Milica S. and Trifković, Jelena  and Cvijetić, Ilija  and Ristivojević, Petar and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "One of the main principles of green analytical chemistry is to eliminate or reduce the use of organic solvents and reagents, and therefore use of Natural Deep Eutectic Solvents (NADES) as medium in sample extraction was recognized as green alternative to conventional one. In this paper, 19 different natural deep eutectic solvents were used for the extraction of phenolic compounds from Teucrium chamaedrys L. Chemical profiles of the extracts were obtained by applying high-performance thin-layer chromatography (HPTLC), which is a green technique due to the simultaneous analysis of a large number of samples, using small amounts of extracts and minimal amounts of solvents. Greenness of proposed methodology was estimated by NEMI pictogram and analytical eco-scale., Jedan od glavnih ciljeva zelene analitičke hemije je eliminisanje ili smanjenje upotrebe organskih rastvarača i reagenasa i zbog toga je primena prirodnih eutektičkih smeša (NADES - natural deep eutectic solvents) kao medijuma za ekstrakciju uzoraka prepoznata kao zelena alternativa. U ovom radu je primenjeno 19 različitih prirodnih eutektičkih smeša za ekstrakciju fenolnih jedinjenja iz biljke podubice. Za hemijsko profilisanje ekstrakata korišćena je visoko-efikasna tankoslojna hromatografija (HPTLC – High-performance thin-layer chromatography) koja predstavlja zelenu tehniku zbog istovremene analize velikog broja uzoraka, uz upotrebu male količine ekstrakata i minimalnih količina rastvarača. Ekološka prihvatljivost predložene metodologije procenjena je primenom Nacionalnog indeksa uticaja metode na životnu sredinu i eko-skale.",
journal = "58. Savetovanje Srpskog hemijskog društva - Knjiga radova",
title = "Green analytical approach for extraction and chemical profiling of phenolic compounds from natural products: A case study of Teucrium chamaedrys L.",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6215"
}

Lazović, M. Č., Jankov, M. S., Trifković, J., Cvijetić, I., Ristivojević, P.,& Milojković-Opsenica, D.. (2022). Green analytical approach for extraction and chemical profiling of phenolic compounds from natural products: A case study of Teucrium chamaedrys L.. in 58. Savetovanje Srpskog hemijskog društva - Knjiga radova.
https://hdl.handle.net/21.15107/rcub_cherry_6215

Lazović MČ, Jankov MS, Trifković J, Cvijetić I, Ristivojević P, Milojković-Opsenica D. Green analytical approach for extraction and chemical profiling of phenolic compounds from natural products: A case study of Teucrium chamaedrys L.. in 58. Savetovanje Srpskog hemijskog društva - Knjiga radova. 2022;.
https://hdl.handle.net/21.15107/rcub_cherry_6215 .

Lazović, Mila Č., Jankov, Milica S., Trifković, Jelena , Cvijetić, Ilija , Ristivojević, Petar, Milojković-Opsenica, Dušanka, "Green analytical approach for extraction and chemical profiling of phenolic compounds from natural products: A case study of Teucrium chamaedrys L." in 58. Savetovanje Srpskog hemijskog društva - Knjiga radova (2022),
https://hdl.handle.net/21.15107/rcub_cherry_6215 .

TY  - CONF
AU  - Lazović, Mila Č.
AU  - Ristivojević, Petar
AU  - Trifković, Jelena 
AU  - Milojković-Opsenica, Dušanka
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6216
AB  - Natural Deep Eutectic Solvents (NADES) are new generation of ionic liquids, and promising solvents in green extraction. NADES are made by mixing two or more compounds, hydrogen bond acceptor (HBA) and hydrogen bond donor (HBD), at various molar ratios.1,2 In this paper 28 different NADES are used as eco-friendly alternatives for extraction of phenolic acids, flavonoids and anthocyanins from chokeberry with the aim of extraction efficiency screening. Twelve different compounds were used for preparation of NADES, among them choline-chloride, proline, urea, glycerol, malic acid, tartaric acid, glucose, fructose, etc. Ultrasound extraction was applied as an eco-friendly and highly-efficient technique for isolation of phenolics and solid phase extraction (SPE) was used for purification of extracts and removal of NADES compounds. Different extraction parameters were optimized: solid/ liquid ratio, volume of added water and loading volume of extracts on SPE. Repeatability of extraction procedure was assessed. Chemical profiles of the extracts were obtained by applying high-performance thin-layer chromatography (HPTLC) and mobile phase was optimized for simultaneously separation of anthocyanins and other phenolic compounds. Extraction efficiency of NADES were compered to acidified methanol as conventional solvents and 1% citric acid in water (w/v) which is a generally recognized as safe (GRAS) solvent. Principal component analysis (PCA) was performed to determine the similarity/dissimilarity between studied NADES and identify most important compounds (bands) for separation between them. PCA illustrate that extraction greatly depends on the compositions of eutectic mixture and shows that NADES can be “designer solvents”.
C3  - 8th Conference of Young Chemists of Serbia, Belgrade, Serbia, 29th October, 2022. In: Book of Abstracts
T1  - Green extraction of bioactive compounds from chokeberry: screening of Natural Deep Eutectic Solvents and chemical profiling
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6216
ER  - 

@conference{
author = "Lazović, Mila Č. and Ristivojević, Petar and Trifković, Jelena  and Milojković-Opsenica, Dušanka",
year = "2022",
abstract = "Natural Deep Eutectic Solvents (NADES) are new generation of ionic liquids, and promising solvents in green extraction. NADES are made by mixing two or more compounds, hydrogen bond acceptor (HBA) and hydrogen bond donor (HBD), at various molar ratios.1,2 In this paper 28 different NADES are used as eco-friendly alternatives for extraction of phenolic acids, flavonoids and anthocyanins from chokeberry with the aim of extraction efficiency screening. Twelve different compounds were used for preparation of NADES, among them choline-chloride, proline, urea, glycerol, malic acid, tartaric acid, glucose, fructose, etc. Ultrasound extraction was applied as an eco-friendly and highly-efficient technique for isolation of phenolics and solid phase extraction (SPE) was used for purification of extracts and removal of NADES compounds. Different extraction parameters were optimized: solid/ liquid ratio, volume of added water and loading volume of extracts on SPE. Repeatability of extraction procedure was assessed. Chemical profiles of the extracts were obtained by applying high-performance thin-layer chromatography (HPTLC) and mobile phase was optimized for simultaneously separation of anthocyanins and other phenolic compounds. Extraction efficiency of NADES were compered to acidified methanol as conventional solvents and 1% citric acid in water (w/v) which is a generally recognized as safe (GRAS) solvent. Principal component analysis (PCA) was performed to determine the similarity/dissimilarity between studied NADES and identify most important compounds (bands) for separation between them. PCA illustrate that extraction greatly depends on the compositions of eutectic mixture and shows that NADES can be “designer solvents”.",
journal = "8th Conference of Young Chemists of Serbia, Belgrade, Serbia, 29th October, 2022. In: Book of Abstracts",
title = "Green extraction of bioactive compounds from chokeberry: screening of Natural Deep Eutectic Solvents and chemical profiling",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6216"
}

Lazović, M. Č., Ristivojević, P., Trifković, J.,& Milojković-Opsenica, D.. (2022). Green extraction of bioactive compounds from chokeberry: screening of Natural Deep Eutectic Solvents and chemical profiling. in 8th Conference of Young Chemists of Serbia, Belgrade, Serbia, 29th October, 2022. In: Book of Abstracts.
https://hdl.handle.net/21.15107/rcub_cherry_6216

Lazović MČ, Ristivojević P, Trifković J, Milojković-Opsenica D. Green extraction of bioactive compounds from chokeberry: screening of Natural Deep Eutectic Solvents and chemical profiling. in 8th Conference of Young Chemists of Serbia, Belgrade, Serbia, 29th October, 2022. In: Book of Abstracts. 2022;.
https://hdl.handle.net/21.15107/rcub_cherry_6216 .

Lazović, Mila Č., Ristivojević, Petar, Trifković, Jelena , Milojković-Opsenica, Dušanka, "Green extraction of bioactive compounds from chokeberry: screening of Natural Deep Eutectic Solvents and chemical profiling" in 8th Conference of Young Chemists of Serbia, Belgrade, Serbia, 29th October, 2022. In: Book of Abstracts (2022),
https://hdl.handle.net/21.15107/rcub_cherry_6216 .