TY  - JOUR
AU  - Grujić, Marica
AU  - Dojnov, Biljana
AU  - Potočnik, Ivana
AU  - Atanasova, Lea
AU  - Duduk, Bojan
AU  - Srebotnik, Ewald
AU  - Druzhinina, Irirna S.
AU  - Kubicek, Christian P.
AU  - Vujčić, Zoran
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3768
AB  - Lignocellulosic plant biomass is the world’s most abundant carbon source and has consequently attracted attention as a renewable resource for production of biofuels and commodity chemicals that could replace fossil resources. Due to its recalcitrant nature, it must be pretreated by chemical, physical or biological means prior to hydrolysis, introducing additional costs. In this paper, we tested the hypothesis that fungi which thrive on lignocellulosic material (straw, bark or soil) would be efficient in degrading untreated lignocellulose. Wheat straw was used as a model. We developed a fast and simple screening method for cellulase producers and tested one hundred Trichoderma strains isolated from wheat straw. The most potent strain—UB483FTG2/ TUCIM 4455, was isolated from substrate used for mushroom cultivation and was identified as T. guizhouense. After optimization of growth medium, high cellulase activity was already achieved after 72 h of fermentation on raw wheat straw, while the model cellulase overproducing strain T. reesei QM 9414 took 170 h and reached only 45% of the cellulase activity secreted by T. guizhouense. Maximum production levels were 1.1 U/mL (measured with CMC as cellulase substrate) and 0.7 U/mL (β-glucosidase assay). The T. guizhouense cellulase cocktail hydrolyzed raw wheat straw within 35 h. Our study shows that screening for fungi that successfully compete for special substrates in nature will lead to the isolation of strains with qualitatively and quantitatively superior enzymes needed for their digestion which could be used for industrial purposes.
PB  - Springer Link
T2  - World Journal of Microbiology and Biotechnology
T1  - Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw
VL  - 35
IS  - 12
DO  - 10.1007/s11274-019-2774-y
ER  - 

@article{
author = "Grujić, Marica and Dojnov, Biljana and Potočnik, Ivana and Atanasova, Lea and Duduk, Bojan and Srebotnik, Ewald and Druzhinina, Irirna S. and Kubicek, Christian P. and Vujčić, Zoran",
year = "2019",
abstract = "Lignocellulosic plant biomass is the world’s most abundant carbon source and has consequently attracted attention as a renewable resource for production of biofuels and commodity chemicals that could replace fossil resources. Due to its recalcitrant nature, it must be pretreated by chemical, physical or biological means prior to hydrolysis, introducing additional costs. In this paper, we tested the hypothesis that fungi which thrive on lignocellulosic material (straw, bark or soil) would be efficient in degrading untreated lignocellulose. Wheat straw was used as a model. We developed a fast and simple screening method for cellulase producers and tested one hundred Trichoderma strains isolated from wheat straw. The most potent strain—UB483FTG2/ TUCIM 4455, was isolated from substrate used for mushroom cultivation and was identified as T. guizhouense. After optimization of growth medium, high cellulase activity was already achieved after 72 h of fermentation on raw wheat straw, while the model cellulase overproducing strain T. reesei QM 9414 took 170 h and reached only 45% of the cellulase activity secreted by T. guizhouense. Maximum production levels were 1.1 U/mL (measured with CMC as cellulase substrate) and 0.7 U/mL (β-glucosidase assay). The T. guizhouense cellulase cocktail hydrolyzed raw wheat straw within 35 h. Our study shows that screening for fungi that successfully compete for special substrates in nature will lead to the isolation of strains with qualitatively and quantitatively superior enzymes needed for their digestion which could be used for industrial purposes.",
publisher = "Springer Link",
journal = "World Journal of Microbiology and Biotechnology",
title = "Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw",
volume = "35",
number = "12",
doi = "10.1007/s11274-019-2774-y"
}

Grujić, M., Dojnov, B., Potočnik, I., Atanasova, L., Duduk, B., Srebotnik, E., Druzhinina, I. S., Kubicek, C. P.,& Vujčić, Z.. (2019). Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw. in World Journal of Microbiology and Biotechnology
Springer Link., 35(12).
https://doi.org/10.1007/s11274-019-2774-y

Grujić M, Dojnov B, Potočnik I, Atanasova L, Duduk B, Srebotnik E, Druzhinina IS, Kubicek CP, Vujčić Z. Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw. in World Journal of Microbiology and Biotechnology. 2019;35(12).
doi:10.1007/s11274-019-2774-y .

Grujić, Marica, Dojnov, Biljana, Potočnik, Ivana, Atanasova, Lea, Duduk, Bojan, Srebotnik, Ewald, Druzhinina, Irirna S., Kubicek, Christian P., Vujčić, Zoran, "Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw" in World Journal of Microbiology and Biotechnology, 35, no. 12 (2019),
https://doi.org/10.1007/s11274-019-2774-y . .

TY  - JOUR
AU  - Petric, Marija
AU  - Subotic, Angelina
AU  - Jevremovic, Sladana
AU  - Trifunović-Momcilov, Milana
AU  - Tadić, Vojin
AU  - Grujić, Marica
AU  - Vujčić, Zoran
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2543
AB  - The aim of this study was to determine the enzymatic profile of esterases and peroxidases during early stages of somatic embryogenesis of Fritillaria meleagris L. Somatic embryogenesis was induced using the leaf base as explant on a medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D). Zymography showed the presence of different moieties, six isoforms of esterases and peroxidases, during morphogenesis as compared to control explants. One isoform of esterases was detected only during the process of somatic embryogenesis, and one isoform was detected in control explants. Analysis of esterases with 1-naphthyl butyrate proved that esterases, which participate in somatic embryogenesis of F. meleagris, belong to the family of aryl esterases. For the first time it was proved that five isoforms of esterases, which are involved in morphogenesis of F. meleagris, belong to the family of aryl esterases, while two isoforms are carboxyl esterases. One isoform of carboxyl esterases was visible in control explants. This is also the first description of peroxidases during the morphogenetic process, and of the difference between aryl and carboxyl esterases. More isoforms of esterases during morphogenesis as compared to control explants are probably responsible for some early physiological process during somatic embryogenesis of F. meleagris.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of biological sciences
T1  - Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base
VL  - 69
IS  - 4
SP  - 619
EP  - 625
DO  - 10.2298/ABS161130007P
ER  - 

@article{
author = "Petric, Marija and Subotic, Angelina and Jevremovic, Sladana and Trifunović-Momcilov, Milana and Tadić, Vojin and Grujić, Marica and Vujčić, Zoran",
year = "2017",
abstract = "The aim of this study was to determine the enzymatic profile of esterases and peroxidases during early stages of somatic embryogenesis of Fritillaria meleagris L. Somatic embryogenesis was induced using the leaf base as explant on a medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D). Zymography showed the presence of different moieties, six isoforms of esterases and peroxidases, during morphogenesis as compared to control explants. One isoform of esterases was detected only during the process of somatic embryogenesis, and one isoform was detected in control explants. Analysis of esterases with 1-naphthyl butyrate proved that esterases, which participate in somatic embryogenesis of F. meleagris, belong to the family of aryl esterases. For the first time it was proved that five isoforms of esterases, which are involved in morphogenesis of F. meleagris, belong to the family of aryl esterases, while two isoforms are carboxyl esterases. One isoform of carboxyl esterases was visible in control explants. This is also the first description of peroxidases during the morphogenetic process, and of the difference between aryl and carboxyl esterases. More isoforms of esterases during morphogenesis as compared to control explants are probably responsible for some early physiological process during somatic embryogenesis of F. meleagris.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of biological sciences",
title = "Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base",
volume = "69",
number = "4",
pages = "619-625",
doi = "10.2298/ABS161130007P"
}

Petric, M., Subotic, A., Jevremovic, S., Trifunović-Momcilov, M., Tadić, V., Grujić, M.,& Vujčić, Z.. (2017). Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base. in Archives of biological sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 69(4), 619-625.
https://doi.org/10.2298/ABS161130007P

Petric M, Subotic A, Jevremovic S, Trifunović-Momcilov M, Tadić V, Grujić M, Vujčić Z. Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base. in Archives of biological sciences. 2017;69(4):619-625.
doi:10.2298/ABS161130007P .

Petric, Marija, Subotic, Angelina, Jevremovic, Sladana, Trifunović-Momcilov, Milana, Tadić, Vojin, Grujić, Marica, Vujčić, Zoran, "Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base" in Archives of biological sciences, 69, no. 4 (2017):619-625,
https://doi.org/10.2298/ABS161130007P . .

TY  - THES
AU  - Grujić, Marica
PY  - 2016
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=4911
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:15379/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48824079
UR  - http://nardus.mpn.gov.rs/123456789/8029
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2720
AB  - Ova disertacija se bavi ispitivanjem mogućnosti primene izolata gljive rodaTrichoderma harzianum za produkciju enzima celulaznog kompleksa, optimizacijomuslova za njihovu produkciju upotrebom otpadnih lignoceluloznih materijala, ispitivanjemmehanizma kontorole produkcije i karakterizacijom produkovanih celulaznih enzima.Razvijen je brz i pouzdan test za selekciju endocelulaznih hiperprodukujućihizolata Trichoderma spp., koji je korišćen za analizu sto Trichoderma spp. izolata.Obećavajući produceri su identifikovani do nivoa vrste. Najbolji producer endocelulaza jeidentifikovan kao Trichoderma guizhouensis (UB483FTH2) iz grupe T. harzianum ikorišćen je za produkciju celulaznih enzima.Optimizovani su uslovi za produkciju celulaznog kompleksa enzima u uslovimatečne fermentacije upotrebom slame kao inducibilnog supstrata. Maksimalna produkcijaendocelulaza (28,32 U/g) je dobijena u 3 danu fermentacije, egzocelulaza (0,049 U/g)nakon 5 dana fermentacije i β-glukozidaza (14,1 U/g) u 7 danu fermentacije.Razvijena je zimogramska metoda za istovremenu detekciju različitih klasacelulaznih izoformi, nakon izoelekrofokusiranja. β-glukozidaze su detektovane nakonprintovanja na nitroceluloznoj membrani upotrebom eskulina. Endocelulaze sudetektovane na poliakrilamidnom gelu sa koopolimerizovanom karboski metil celulozom,dok su egzocelulaze detektovane upotrebom 4-metilumberiferil-β-D-celobiozida kaosupstrata.Ispitana je i upotreba iskorišćenog komposta šampinjona kao novog supstrata zaprodukciju celulaznih enzima gajenjem 6 izolata Trichoderma spp. Produkovana jeznačajna količina endocelulaza i β-glukozidaza, uz istovremeno smanjenje početnogsupstrata za 30%.Mehanizam regulacije produkcije celulaznih proteina izolata UB483FTH2 jeanaliziran korelisanjem nivoa transkribovane iRNA za gene xyr1 i lae1, regulatoraprodukcije celulaza, sa produkcijom svih celulaznih enzima. Pronađeno je da eksprimiranigeni, xyr1 i lae1, imaju pozitivan uticaj na produkciju celulaznih enzima i proteina.
AB  - This thesis examines application of isolates of Trichoderma harzianum forproduction of cellulase enzyme complex, optimization of their production usinglignocellulose waste materials, studying of control mechanisms for production andcharacterization of the produced cellulases enzymes.Fast and reliable test for the selection of endocellulase hyper-producing isolates ofTrichoderma spp. has been developed and used for screening one hundred isolates ofTrichoderma spp. The promising isolates have been isolated to species level. The bestendocellulase producer is identified as Trichoderma guizhouensis (UB483FTH) whichbelongs to the Trichoderma harzianum species complex. This strains was used forproduction of cellulase enzyme complex.Cellulase production was optimized using wheat straw as a substrate in conditionsof submerged fermentation. Maximum of endocelullase production (28.32 U/g) wasobtained in 3rd days of fermentation, exocellulase (0.049 U/g) after 5th days and β-glucosidase (14.1 U/g) in 7th days of fermentation.Reliable zymographic method for simultaneous characterization of cellulolyticcomplex enzymes after isoelectric focusing has been developed. β-glucosidase is detectedafter printing on nitrocellulose membrane using esculin as substrate. Endocellulase wasdetected on polyacrilamide gel with copolymerized carboxymethyl-cellulose andexocellulase were detected using 4-metillumberiferil-β-D-celobioside as a substrate.Reuse of spent mushroom compost as a new substrate for the production ofcellulase enzymes by 6 isolates Trichoderma spp. was examined here too. Significantamount of endocellulase and β-glucosidase were produced, while the amount of initialsubstrate decreased by 30%.The regulation mechanism of protein production of isolate UB483FTH2 analysedas a correlation of transcribed level of mRNA for genes xyr1 and lae1 as a regulators ofproduction of cellulase enzymes. It was found that expressed genes xyr1 and lae1 have apositive effect on the production of cellulases proteins and total proteins.
PB  - Универзитет у Београду, Хемијски факултет
T2  - Универзитет у Београду
T1  - Celulaze gljive Trichoderma harzianum: produkcija, kontrola produkcije i karakterizacija eksprimiranih enzima
UR  - https://hdl.handle.net/21.15107/rcub_nardus_8029
ER  - 

@phdthesis{
author = "Grujić, Marica",
year = "2016",
abstract = "Ova disertacija se bavi ispitivanjem mogućnosti primene izolata gljive rodaTrichoderma harzianum za produkciju enzima celulaznog kompleksa, optimizacijomuslova za njihovu produkciju upotrebom otpadnih lignoceluloznih materijala, ispitivanjemmehanizma kontorole produkcije i karakterizacijom produkovanih celulaznih enzima.Razvijen je brz i pouzdan test za selekciju endocelulaznih hiperprodukujućihizolata Trichoderma spp., koji je korišćen za analizu sto Trichoderma spp. izolata.Obećavajući produceri su identifikovani do nivoa vrste. Najbolji producer endocelulaza jeidentifikovan kao Trichoderma guizhouensis (UB483FTH2) iz grupe T. harzianum ikorišćen je za produkciju celulaznih enzima.Optimizovani su uslovi za produkciju celulaznog kompleksa enzima u uslovimatečne fermentacije upotrebom slame kao inducibilnog supstrata. Maksimalna produkcijaendocelulaza (28,32 U/g) je dobijena u 3 danu fermentacije, egzocelulaza (0,049 U/g)nakon 5 dana fermentacije i β-glukozidaza (14,1 U/g) u 7 danu fermentacije.Razvijena je zimogramska metoda za istovremenu detekciju različitih klasacelulaznih izoformi, nakon izoelekrofokusiranja. β-glukozidaze su detektovane nakonprintovanja na nitroceluloznoj membrani upotrebom eskulina. Endocelulaze sudetektovane na poliakrilamidnom gelu sa koopolimerizovanom karboski metil celulozom,dok su egzocelulaze detektovane upotrebom 4-metilumberiferil-β-D-celobiozida kaosupstrata.Ispitana je i upotreba iskorišćenog komposta šampinjona kao novog supstrata zaprodukciju celulaznih enzima gajenjem 6 izolata Trichoderma spp. Produkovana jeznačajna količina endocelulaza i β-glukozidaza, uz istovremeno smanjenje početnogsupstrata za 30%.Mehanizam regulacije produkcije celulaznih proteina izolata UB483FTH2 jeanaliziran korelisanjem nivoa transkribovane iRNA za gene xyr1 i lae1, regulatoraprodukcije celulaza, sa produkcijom svih celulaznih enzima. Pronađeno je da eksprimiranigeni, xyr1 i lae1, imaju pozitivan uticaj na produkciju celulaznih enzima i proteina., This thesis examines application of isolates of Trichoderma harzianum forproduction of cellulase enzyme complex, optimization of their production usinglignocellulose waste materials, studying of control mechanisms for production andcharacterization of the produced cellulases enzymes.Fast and reliable test for the selection of endocellulase hyper-producing isolates ofTrichoderma spp. has been developed and used for screening one hundred isolates ofTrichoderma spp. The promising isolates have been isolated to species level. The bestendocellulase producer is identified as Trichoderma guizhouensis (UB483FTH) whichbelongs to the Trichoderma harzianum species complex. This strains was used forproduction of cellulase enzyme complex.Cellulase production was optimized using wheat straw as a substrate in conditionsof submerged fermentation. Maximum of endocelullase production (28.32 U/g) wasobtained in 3rd days of fermentation, exocellulase (0.049 U/g) after 5th days and β-glucosidase (14.1 U/g) in 7th days of fermentation.Reliable zymographic method for simultaneous characterization of cellulolyticcomplex enzymes after isoelectric focusing has been developed. β-glucosidase is detectedafter printing on nitrocellulose membrane using esculin as substrate. Endocellulase wasdetected on polyacrilamide gel with copolymerized carboxymethyl-cellulose andexocellulase were detected using 4-metillumberiferil-β-D-celobioside as a substrate.Reuse of spent mushroom compost as a new substrate for the production ofcellulase enzymes by 6 isolates Trichoderma spp. was examined here too. Significantamount of endocellulase and β-glucosidase were produced, while the amount of initialsubstrate decreased by 30%.The regulation mechanism of protein production of isolate UB483FTH2 analysedas a correlation of transcribed level of mRNA for genes xyr1 and lae1 as a regulators ofproduction of cellulase enzymes. It was found that expressed genes xyr1 and lae1 have apositive effect on the production of cellulases proteins and total proteins.",
publisher = "Универзитет у Београду, Хемијски факултет",
journal = "Универзитет у Београду",
title = "Celulaze gljive Trichoderma harzianum: produkcija, kontrola produkcije i karakterizacija eksprimiranih enzima",
url = "https://hdl.handle.net/21.15107/rcub_nardus_8029"
}

Grujić, M.. (2016). Celulaze gljive Trichoderma harzianum: produkcija, kontrola produkcije i karakterizacija eksprimiranih enzima. in Универзитет у Београду
Универзитет у Београду, Хемијски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_8029

Grujić M. Celulaze gljive Trichoderma harzianum: produkcija, kontrola produkcije i karakterizacija eksprimiranih enzima. in Универзитет у Београду. 2016;.
https://hdl.handle.net/21.15107/rcub_nardus_8029 .

Grujić, Marica, "Celulaze gljive Trichoderma harzianum: produkcija, kontrola produkcije i karakterizacija eksprimiranih enzima" in Универзитет у Београду (2016),
https://hdl.handle.net/21.15107/rcub_nardus_8029 .

TY  - JOUR
AU  - Dojnov, Biljana
AU  - Grujić, Marica
AU  - Vujčić, Zoran
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3408
AB  - A method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4-methylumbelliferyl--d-cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates.
PB  - Wiley-Blackwell, Hoboken
T2  - Electrophoresis
T1  - Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex
VL  - 36
IS  - 15
SP  - 1724
EP  - 1727
DO  - 10.1002/elps.201400541
ER  - 

@article{
author = "Dojnov, Biljana and Grujić, Marica and Vujčić, Zoran",
year = "2015",
abstract = "A method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4-methylumbelliferyl--d-cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Electrophoresis",
title = "Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex",
volume = "36",
number = "15",
pages = "1724-1727",
doi = "10.1002/elps.201400541"
}

Dojnov, B., Grujić, M.,& Vujčić, Z.. (2015). Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex. in Electrophoresis
Wiley-Blackwell, Hoboken., 36(15), 1724-1727.
https://doi.org/10.1002/elps.201400541

Dojnov B, Grujić M, Vujčić Z. Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex. in Electrophoresis. 2015;36(15):1724-1727.
doi:10.1002/elps.201400541 .

Dojnov, Biljana, Grujić, Marica, Vujčić, Zoran, "Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex" in Electrophoresis, 36, no. 15 (2015):1724-1727,
https://doi.org/10.1002/elps.201400541 . .

TY  - JOUR
AU  - Dojnov, Biljana
AU  - Grujić, Marica
AU  - Percevic, Bojana
AU  - Vujčić, Zoran
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1993
AB  - With the purpose of finding a suitable available inducer in combination with starvation, carbohydrate mixtures from triticale were used and compared with well-known amylase inducers in fungi. Carbohydrate mixtures from triticale induced the production of an amylase cocktail (alpha-amylase and glucoamylase) in Aspergillus niger, unlike induction with well-known inducers that induce only glucoamylase, shown by zymography and TLC analysis of the carbohydrate mixtures before and after fermentation. Glucoamylase production by A. niger was the highest in the presence of the extract obtained after auto-hydrolysis of starch from triticale (95.88 U mL(-1)). Carbohydrate mixtures from triticale induced the production of alpha-amylase in A. oryzae. More alpha-amylase isoforms were detected when using a complex carbohydrate mixture, compared to induction with maltose or starch. A 48-h induction was the most efficient using a triticale extract (101.35 U mL(-1)). Carbohydrates from triticale extracts could be used as very good cheap amylase inducers. Triticale, still not fully utilized, could be taken into consideration as an inducer in amylase production by Aspergillus sp, and in such a way, it could be used as the sole substrate in fermentation.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Enhancement of amylase production by Aspergillus sp using carbohydrates mixtures from triticale
VL  - 80
IS  - 10
SP  - 1279
EP  - 1288
DO  - 10.2298/JSC150317043D
ER  - 

@article{
author = "Dojnov, Biljana and Grujić, Marica and Percevic, Bojana and Vujčić, Zoran",
year = "2015",
abstract = "With the purpose of finding a suitable available inducer in combination with starvation, carbohydrate mixtures from triticale were used and compared with well-known amylase inducers in fungi. Carbohydrate mixtures from triticale induced the production of an amylase cocktail (alpha-amylase and glucoamylase) in Aspergillus niger, unlike induction with well-known inducers that induce only glucoamylase, shown by zymography and TLC analysis of the carbohydrate mixtures before and after fermentation. Glucoamylase production by A. niger was the highest in the presence of the extract obtained after auto-hydrolysis of starch from triticale (95.88 U mL(-1)). Carbohydrate mixtures from triticale induced the production of alpha-amylase in A. oryzae. More alpha-amylase isoforms were detected when using a complex carbohydrate mixture, compared to induction with maltose or starch. A 48-h induction was the most efficient using a triticale extract (101.35 U mL(-1)). Carbohydrates from triticale extracts could be used as very good cheap amylase inducers. Triticale, still not fully utilized, could be taken into consideration as an inducer in amylase production by Aspergillus sp, and in such a way, it could be used as the sole substrate in fermentation.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Enhancement of amylase production by Aspergillus sp using carbohydrates mixtures from triticale",
volume = "80",
number = "10",
pages = "1279-1288",
doi = "10.2298/JSC150317043D"
}

Dojnov, B., Grujić, M., Percevic, B.,& Vujčić, Z.. (2015). Enhancement of amylase production by Aspergillus sp using carbohydrates mixtures from triticale. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 80(10), 1279-1288.
https://doi.org/10.2298/JSC150317043D

Dojnov B, Grujić M, Percevic B, Vujčić Z. Enhancement of amylase production by Aspergillus sp using carbohydrates mixtures from triticale. in Journal of the Serbian Chemical Society. 2015;80(10):1279-1288.
doi:10.2298/JSC150317043D .

Dojnov, Biljana, Grujić, Marica, Percevic, Bojana, Vujčić, Zoran, "Enhancement of amylase production by Aspergillus sp using carbohydrates mixtures from triticale" in Journal of the Serbian Chemical Society, 80, no. 10 (2015):1279-1288,
https://doi.org/10.2298/JSC150317043D . .

TY  - JOUR
AU  - Dojnov, Biljana
AU  - Grujić, Marica
AU  - Vujčić, Zoran
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2011
AB  - Triticale (x Triticosecale, Wittmack), an important industrial crop, with high grain yield, containing high amounts of starch, proteins and also major and minor mineral elements, is not yet sufficiently utilized. The simultaneous production of alpha-amylase and glucoamylase isoforms by Aspergillus niger on triticale grains, without any nutritive supplements, was developed, optimized and scaled up 10 fold for the first time. The specific combination of the examined effects led to the production of a novel glucoamylase isoform. Reduction of particle size, increase in oxygen availability and substrate height lead to an increase of 30 % in the production of amylases. Reduction of the relative humidity from 65 to 30 % increased glucoamylase production 2 fold and alpha-amylase production by 30%. The peak production of alpha-amylase (158 U g(-1)) and glucoamylase (170 U g(-1)) were obtained in Erlenmeyer flasks and in scaled-up trays. The obtained A. niger amylase cocktail was more efficient in raw starch hydrolysis from wheat flour, 29 % more efficient in glucose formation and 10 % more efficient in total reducing sugar formation, than the commercially available amylase cocktail SAN Super 240L, which is widely used in industry.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate
VL  - 80
IS  - 11
SP  - 1375
EP  - 1390
DO  - 10.2298/JSC150317041D
ER  - 

@article{
author = "Dojnov, Biljana and Grujić, Marica and Vujčić, Zoran",
year = "2015",
abstract = "Triticale (x Triticosecale, Wittmack), an important industrial crop, with high grain yield, containing high amounts of starch, proteins and also major and minor mineral elements, is not yet sufficiently utilized. The simultaneous production of alpha-amylase and glucoamylase isoforms by Aspergillus niger on triticale grains, without any nutritive supplements, was developed, optimized and scaled up 10 fold for the first time. The specific combination of the examined effects led to the production of a novel glucoamylase isoform. Reduction of particle size, increase in oxygen availability and substrate height lead to an increase of 30 % in the production of amylases. Reduction of the relative humidity from 65 to 30 % increased glucoamylase production 2 fold and alpha-amylase production by 30%. The peak production of alpha-amylase (158 U g(-1)) and glucoamylase (170 U g(-1)) were obtained in Erlenmeyer flasks and in scaled-up trays. The obtained A. niger amylase cocktail was more efficient in raw starch hydrolysis from wheat flour, 29 % more efficient in glucose formation and 10 % more efficient in total reducing sugar formation, than the commercially available amylase cocktail SAN Super 240L, which is widely used in industry.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate",
volume = "80",
number = "11",
pages = "1375-1390",
doi = "10.2298/JSC150317041D"
}

Dojnov, B., Grujić, M.,& Vujčić, Z.. (2015). Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 80(11), 1375-1390.
https://doi.org/10.2298/JSC150317041D

Dojnov B, Grujić M, Vujčić Z. Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate. in Journal of the Serbian Chemical Society. 2015;80(11):1375-1390.
doi:10.2298/JSC150317041D .

Dojnov, Biljana, Grujić, Marica, Vujčić, Zoran, "Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate" in Journal of the Serbian Chemical Society, 80, no. 11 (2015):1375-1390,
https://doi.org/10.2298/JSC150317041D . .

TY  - JOUR
AU  - Dojnov, Biljana
AU  - Grujić, Marica
AU  - Vujčić, Zoran
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1748
AB  - A method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4-methylumbelliferyl--d-cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates.
PB  - Wiley-Blackwell, Hoboken
T2  - Electrophoresis
T1  - Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex
VL  - 36
IS  - 15
SP  - 1724
EP  - 1727
DO  - 10.1002/elps.201400541
ER  - 

@article{
author = "Dojnov, Biljana and Grujić, Marica and Vujčić, Zoran",
year = "2015",
abstract = "A method for zymographic detection of specific cellulases in a complex (endocellulase, exocellulase, and cellobiase) from crude fermentation extracts, after a single electrophoretic separation, is described in this paper. Cellulases were printed onto a membrane and, subsequently, substrate gel. Cellobiase isoforms were detected on the membrane using esculine as substrate, endocellulase isoforms on substrate gel with copolymerized carboxymethyl cellulose (CMC), while exocellulase isoforms were detected in electrophoresis gel with 4-methylumbelliferyl--d-cellobioside (MUC). This can be a useful additional tool for monitoring and control of fungal cellulase production in industrial processes and fundamental research, screening for particular cellulase producers, or testing of new lignocellulose substrates.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Electrophoresis",
title = "Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex",
volume = "36",
number = "15",
pages = "1724-1727",
doi = "10.1002/elps.201400541"
}

Dojnov, B., Grujić, M.,& Vujčić, Z.. (2015). Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex. in Electrophoresis
Wiley-Blackwell, Hoboken., 36(15), 1724-1727.
https://doi.org/10.1002/elps.201400541

Dojnov B, Grujić M, Vujčić Z. Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex. in Electrophoresis. 2015;36(15):1724-1727.
doi:10.1002/elps.201400541 .

Dojnov, Biljana, Grujić, Marica, Vujčić, Zoran, "Reliable simultaneous zymographic method of characterization of cellulolytic enzymes from fungal cellulase complex" in Electrophoresis, 36, no. 15 (2015):1724-1727,
https://doi.org/10.1002/elps.201400541 . .

TY  - JOUR
AU  - Petric, Marija
AU  - Subotic, Angelina
AU  - Jevremovic, Sladana
AU  - Trifunović-Momcilov, Milana
AU  - Tadić, Vojin
AU  - Grujić, Marica
AU  - Vujčić, Zoran
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2032
AB  - Morphogenesis in vitro is a complex and still poorly defined process. We investigated esterase and peroxidase isoforms detected in bulb scale, during Fritillaria meleagris morphogenesis. Bulbs were grown either at 4 degrees C or on a medium with an increased concentration of sucrose (4.5%) for 30 days. After these pre-treatments, the bulb scales were further grown on nutrient media that contained different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KIN) or thidiazuron (TDZ). Regeneration of somatic embryos and bulblets occurred at the same explant. The highest numbers of somatic embryos and bulblets were regenerated on the medium containing 2,4-D and KIN (1 mg/L each), while morphogenesis was most successful at a TDZ concentration between 0.5 and 1 mg/L. Monitoring of esterases and peroxidases was performed by growing bulb scales on a medium enriched with 2,4-D and KIN or TDZ (1 mg/L), and the number and activity of isoforms were followed every 7 days for 4 weeks. In control explants, six isoforms of esterase were observed. Three isoforms of peroxidase were not detected in the control bulb scale, which has not begun its morphogenesis process. 2015 Academie des sciences. (C) Published by Elsevier Masson SAS. All rights reserved.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Comptes Rendus Biologies
T1  - Esterase and peroxidase isoforms in different stages of morphogenesis in Fritillaria meleagris L. in bulb-scale culture
VL  - 338
IS  - 12
SP  - 793
EP  - 802
DO  - 10.1016/j.crvi.2015.08.002
ER  - 

@article{
author = "Petric, Marija and Subotic, Angelina and Jevremovic, Sladana and Trifunović-Momcilov, Milana and Tadić, Vojin and Grujić, Marica and Vujčić, Zoran",
year = "2015",
abstract = "Morphogenesis in vitro is a complex and still poorly defined process. We investigated esterase and peroxidase isoforms detected in bulb scale, during Fritillaria meleagris morphogenesis. Bulbs were grown either at 4 degrees C or on a medium with an increased concentration of sucrose (4.5%) for 30 days. After these pre-treatments, the bulb scales were further grown on nutrient media that contained different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KIN) or thidiazuron (TDZ). Regeneration of somatic embryos and bulblets occurred at the same explant. The highest numbers of somatic embryos and bulblets were regenerated on the medium containing 2,4-D and KIN (1 mg/L each), while morphogenesis was most successful at a TDZ concentration between 0.5 and 1 mg/L. Monitoring of esterases and peroxidases was performed by growing bulb scales on a medium enriched with 2,4-D and KIN or TDZ (1 mg/L), and the number and activity of isoforms were followed every 7 days for 4 weeks. In control explants, six isoforms of esterase were observed. Three isoforms of peroxidase were not detected in the control bulb scale, which has not begun its morphogenesis process. 2015 Academie des sciences. (C) Published by Elsevier Masson SAS. All rights reserved.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Comptes Rendus Biologies",
title = "Esterase and peroxidase isoforms in different stages of morphogenesis in Fritillaria meleagris L. in bulb-scale culture",
volume = "338",
number = "12",
pages = "793-802",
doi = "10.1016/j.crvi.2015.08.002"
}

Petric, M., Subotic, A., Jevremovic, S., Trifunović-Momcilov, M., Tadić, V., Grujić, M.,& Vujčić, Z.. (2015). Esterase and peroxidase isoforms in different stages of morphogenesis in Fritillaria meleagris L. in bulb-scale culture. in Comptes Rendus Biologies
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 338(12), 793-802.
https://doi.org/10.1016/j.crvi.2015.08.002

Petric M, Subotic A, Jevremovic S, Trifunović-Momcilov M, Tadić V, Grujić M, Vujčić Z. Esterase and peroxidase isoforms in different stages of morphogenesis in Fritillaria meleagris L. in bulb-scale culture. in Comptes Rendus Biologies. 2015;338(12):793-802.
doi:10.1016/j.crvi.2015.08.002 .

Petric, Marija, Subotic, Angelina, Jevremovic, Sladana, Trifunović-Momcilov, Milana, Tadić, Vojin, Grujić, Marica, Vujčić, Zoran, "Esterase and peroxidase isoforms in different stages of morphogenesis in Fritillaria meleagris L. in bulb-scale culture" in Comptes Rendus Biologies, 338, no. 12 (2015):793-802,
https://doi.org/10.1016/j.crvi.2015.08.002 . .

TY  - JOUR
AU  - Grujić, Marica
AU  - Dojnov, Biljana
AU  - Potočnik, Ivana
AU  - Duduk, Bojan
AU  - Vujčić, Zoran
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1974
AB  - Mushroom production is the biggest solid state fermentation industry in the world. Disposal and storage of spent mushroom compost (SMC) that remains after mushroom harvest poses a big economic and environmental problem. Production of industrially important hydrolytic enzymes by fungi on various agro-industrial wastes is a significant, open chapter in biotechnology. This paper proposes a novel application of SMC as substrate for cultivation of fungi in solid state fermentation (SSF) in order to obtain the enzymes cellulase, xylanase, amylase and beta-glucosidase. SMC can be used as a good substrate for cultivation of Trichoderma and Aspergillus without the addition of supplementary (nutritive) elements. Starting amount of SMC was reduced by 30% due to hydrolysis by a complex of cellulolytic enzymes. Material that is left behind is a more suitable fertilizer for horticulture. One fungal isolate was pointed out as a promising producer (Trichoderma atroviride isolate T42). It produced the greatest amount of total protein (0.204 mg mL(-1)), five isoforms of beta-glucosidase and the highest level (12 isoforms) of both endocellulase (0.76 U mL(-1)) and xylanase (2.31 U mL(-1)). The capacity of T42 to produce all examined enzymes in such a high number of isoforms demonstrates successful adaptation to new substrates.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Biodeterioration and Biodegradation
T1  - Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation
VL  - 104
SP  - 290
EP  - 298
DO  - 10.1016/j.ibiod.2015.04.029
ER  - 

@article{
author = "Grujić, Marica and Dojnov, Biljana and Potočnik, Ivana and Duduk, Bojan and Vujčić, Zoran",
year = "2015",
abstract = "Mushroom production is the biggest solid state fermentation industry in the world. Disposal and storage of spent mushroom compost (SMC) that remains after mushroom harvest poses a big economic and environmental problem. Production of industrially important hydrolytic enzymes by fungi on various agro-industrial wastes is a significant, open chapter in biotechnology. This paper proposes a novel application of SMC as substrate for cultivation of fungi in solid state fermentation (SSF) in order to obtain the enzymes cellulase, xylanase, amylase and beta-glucosidase. SMC can be used as a good substrate for cultivation of Trichoderma and Aspergillus without the addition of supplementary (nutritive) elements. Starting amount of SMC was reduced by 30% due to hydrolysis by a complex of cellulolytic enzymes. Material that is left behind is a more suitable fertilizer for horticulture. One fungal isolate was pointed out as a promising producer (Trichoderma atroviride isolate T42). It produced the greatest amount of total protein (0.204 mg mL(-1)), five isoforms of beta-glucosidase and the highest level (12 isoforms) of both endocellulase (0.76 U mL(-1)) and xylanase (2.31 U mL(-1)). The capacity of T42 to produce all examined enzymes in such a high number of isoforms demonstrates successful adaptation to new substrates.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Biodeterioration and Biodegradation",
title = "Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation",
volume = "104",
pages = "290-298",
doi = "10.1016/j.ibiod.2015.04.029"
}

Grujić, M., Dojnov, B., Potočnik, I., Duduk, B.,& Vujčić, Z.. (2015). Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation. in International Biodeterioration and Biodegradation
Elsevier Sci Ltd, Oxford., 104, 290-298.
https://doi.org/10.1016/j.ibiod.2015.04.029

Grujić M, Dojnov B, Potočnik I, Duduk B, Vujčić Z. Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation. in International Biodeterioration and Biodegradation. 2015;104:290-298.
doi:10.1016/j.ibiod.2015.04.029 .

Grujić, Marica, Dojnov, Biljana, Potočnik, Ivana, Duduk, Bojan, Vujčić, Zoran, "Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation" in International Biodeterioration and Biodegradation, 104 (2015):290-298,
https://doi.org/10.1016/j.ibiod.2015.04.029 . .

TY  - JOUR
AU  - Pavlovic, R.
AU  - Grujić, Marica
AU  - Dojnov, Biljana
AU  - Vujčić, Miroslava
AU  - Nenadovic, Vera
AU  - Ivanovic, Jelisaveta
AU  - Vujčić, Zoran
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1300
AB  - The expression and distribution of digestive cellulases along the midgut of Cerambyx cerdo larvae were analyzed for the first time and are presented in this article. Four groups of larvae were examined: larvae developed in the wild; larvae taken from the wild and successively reared on an artificial diet based on polenta; and larvae hatched in the laboratory and reared on two different artificial diets. Seven endocellulase and seven beta-D-glucosidase isoforms were detected in all midgut extracts of C. cerdo with a zymogram after native PAGE. We observed that C. cerdo larvae are capable of producing cellulase isoforms with different PAGE mobilities depending on the nutrient substrate. From our findings it can be assumed that, depending on the distribution of endocellulase and beta-D-glucosidase, cellulose molecules are first fragmented in the anterior and middle midgut by endo-beta-1,4-glucanase; subsequently, the obtained fragments are broken down by beta-D-glucosidase mostly in middle midgut.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of biological sciences
T1  - Influence of Nutrient Substrates on the Expression of Cellulases in Cerambyx Cerdo L. (Coleoptera: Cerambycidae) Larvae
VL  - 64
IS  - 2
SP  - 757
EP  - 765
DO  - 10.2298/ABS1202757P
ER  - 

@article{
author = "Pavlovic, R. and Grujić, Marica and Dojnov, Biljana and Vujčić, Miroslava and Nenadovic, Vera and Ivanovic, Jelisaveta and Vujčić, Zoran",
year = "2012",
abstract = "The expression and distribution of digestive cellulases along the midgut of Cerambyx cerdo larvae were analyzed for the first time and are presented in this article. Four groups of larvae were examined: larvae developed in the wild; larvae taken from the wild and successively reared on an artificial diet based on polenta; and larvae hatched in the laboratory and reared on two different artificial diets. Seven endocellulase and seven beta-D-glucosidase isoforms were detected in all midgut extracts of C. cerdo with a zymogram after native PAGE. We observed that C. cerdo larvae are capable of producing cellulase isoforms with different PAGE mobilities depending on the nutrient substrate. From our findings it can be assumed that, depending on the distribution of endocellulase and beta-D-glucosidase, cellulose molecules are first fragmented in the anterior and middle midgut by endo-beta-1,4-glucanase; subsequently, the obtained fragments are broken down by beta-D-glucosidase mostly in middle midgut.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of biological sciences",
title = "Influence of Nutrient Substrates on the Expression of Cellulases in Cerambyx Cerdo L. (Coleoptera: Cerambycidae) Larvae",
volume = "64",
number = "2",
pages = "757-765",
doi = "10.2298/ABS1202757P"
}

Pavlovic, R., Grujić, M., Dojnov, B., Vujčić, M., Nenadovic, V., Ivanovic, J.,& Vujčić, Z.. (2012). Influence of Nutrient Substrates on the Expression of Cellulases in Cerambyx Cerdo L. (Coleoptera: Cerambycidae) Larvae. in Archives of biological sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 64(2), 757-765.
https://doi.org/10.2298/ABS1202757P

Pavlovic R, Grujić M, Dojnov B, Vujčić M, Nenadovic V, Ivanovic J, Vujčić Z. Influence of Nutrient Substrates on the Expression of Cellulases in Cerambyx Cerdo L. (Coleoptera: Cerambycidae) Larvae. in Archives of biological sciences. 2012;64(2):757-765.
doi:10.2298/ABS1202757P .

Pavlovic, R., Grujić, Marica, Dojnov, Biljana, Vujčić, Miroslava, Nenadovic, Vera, Ivanovic, Jelisaveta, Vujčić, Zoran, "Influence of Nutrient Substrates on the Expression of Cellulases in Cerambyx Cerdo L. (Coleoptera: Cerambycidae) Larvae" in Archives of biological sciences, 64, no. 2 (2012):757-765,
https://doi.org/10.2298/ABS1202757P . .