Vovk, Irena

Link to this page

Authority KeyName Variants
orcid::0000-0002-4738-2849
  • Vovk, Irena (12)
Projects
Structure-properties relationships of natural and synthetic molecules and their metal complexes Slovenian Research Agency [P1-0005]
Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research Molecular characterization of bacteria from genera Bacillus and Pseudomonas as potential agents for biological control
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200051 (Institute of General and Physical Chemistry, Belgrade) Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200168 (University of Belgrade, Faculty of Chemistry)
Slovenian Research Agency (research core funding No. P1-0005) [BI-RS/14-15-007 (451-03-3095/2014-09/14)]
EN-FIST Centre of Excellence ENFIST Centre of Excellence infrastructure (TLC-MS interface).
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM)
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200116 (University of Belgrade, Faculty of Agriculture) The application of new genotypes and technological innovations for the purpose of improvement of fruit-growing and viticultural production
Scientific and Technological Research Council of Turkey (TUBITAK) Slovenian Research Agency (No. P1-0005).
Slovenian Research Agency (research core funding No. P1- 0005). Slovenian Research Agency (research program P1-0005 “Functional food and food supplements”
specialized funds of the Department of Environmental Health, Faculty of Medicine, University of Rijeka

Author's Bibliography

The Role of Isoflavones in the Prevention of Breast Cancer and Prostate Cancer

Pejčić, Tomislav; Zeković, Milica; Bumbaširević, Uroš; Kalaba, Milica; Vovk, Irena; Bensa, Maja; Popović, Lazar; Tešić, Živoslav Lj.

(MDPI, 2023)

TY  - JOUR
AU  - Pejčić, Tomislav
AU  - Zeković, Milica
AU  - Bumbaširević, Uroš
AU  - Kalaba, Milica
AU  - Vovk, Irena
AU  - Bensa, Maja
AU  - Popović, Lazar
AU  - Tešić, Živoslav Lj.
PY  - 2023
UR  - https://www.mdpi.com/2076-3921/12/2/368
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6181
AB  - This narrative review summarizes epidemiological studies on breast cancer and prostate cancer with an overview of their global incidence distribution to investigate the relationship between these diseases and diet. The biological properties, mechanisms of action, and available data supporting the potential role of isoflavones in the prevention of breast cancer and prostate cancer are discussed. Studies evaluating the effects of isoflavones in tissue cultures of normal and malignant breast and prostate cells, as well as the current body of research regarding the effects of isoflavones attained through multiple modifications of cellular molecular signaling pathways and control of oxidative stress, are summarized. Furthermore, this review compiles literature sources reporting on the following: (1) levels of estrogen in breast and prostate tissue; (2) levels of isoflavones in the normal and malignant tissue of these organs in European and Asian populations; (3) average concentrations of isoflavones in the secretion of these organs (milk and semen). Finally, particular emphasis is placed on studies investigating the effect of isoflavones on tissues via estrogen receptors (ER).
PB  - MDPI
T2  - Antioxidants
T1  - The Role of Isoflavones in the Prevention of Breast Cancer and Prostate Cancer
VL  - 12
IS  - 2
SP  - 368
DO  - 10.3390/antiox12020368
ER  - 
@article{
author = "Pejčić, Tomislav and Zeković, Milica and Bumbaširević, Uroš and Kalaba, Milica and Vovk, Irena and Bensa, Maja and Popović, Lazar and Tešić, Živoslav Lj.",
year = "2023",
abstract = "This narrative review summarizes epidemiological studies on breast cancer and prostate cancer with an overview of their global incidence distribution to investigate the relationship between these diseases and diet. The biological properties, mechanisms of action, and available data supporting the potential role of isoflavones in the prevention of breast cancer and prostate cancer are discussed. Studies evaluating the effects of isoflavones in tissue cultures of normal and malignant breast and prostate cells, as well as the current body of research regarding the effects of isoflavones attained through multiple modifications of cellular molecular signaling pathways and control of oxidative stress, are summarized. Furthermore, this review compiles literature sources reporting on the following: (1) levels of estrogen in breast and prostate tissue; (2) levels of isoflavones in the normal and malignant tissue of these organs in European and Asian populations; (3) average concentrations of isoflavones in the secretion of these organs (milk and semen). Finally, particular emphasis is placed on studies investigating the effect of isoflavones on tissues via estrogen receptors (ER).",
publisher = "MDPI",
journal = "Antioxidants",
title = "The Role of Isoflavones in the Prevention of Breast Cancer and Prostate Cancer",
volume = "12",
number = "2",
pages = "368",
doi = "10.3390/antiox12020368"
}
Pejčić, T., Zeković, M., Bumbaširević, U., Kalaba, M., Vovk, I., Bensa, M., Popović, L.,& Tešić, Ž. Lj.. (2023). The Role of Isoflavones in the Prevention of Breast Cancer and Prostate Cancer. in Antioxidants
MDPI., 12(2), 368.
https://doi.org/10.3390/antiox12020368
Pejčić T, Zeković M, Bumbaširević U, Kalaba M, Vovk I, Bensa M, Popović L, Tešić ŽL. The Role of Isoflavones in the Prevention of Breast Cancer and Prostate Cancer. in Antioxidants. 2023;12(2):368.
doi:10.3390/antiox12020368 .
Pejčić, Tomislav, Zeković, Milica, Bumbaširević, Uroš, Kalaba, Milica, Vovk, Irena, Bensa, Maja, Popović, Lazar, Tešić, Živoslav Lj., "The Role of Isoflavones in the Prevention of Breast Cancer and Prostate Cancer" in Antioxidants, 12, no. 2 (2023):368,
https://doi.org/10.3390/antiox12020368 . .
5
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Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia

Nedić, Nebojša; Nešović, Milica; Radišić, Predrag; Gašić, Uroš M.; Baošić, Rada; Joksimović, Kristina; Pezo, Lato; Tešić, Živoslav Lj.; Vovk, Irena

(Frontiers Media S.A., 2022)

TY  - JOUR
AU  - Nedić, Nebojša
AU  - Nešović, Milica
AU  - Radišić, Predrag
AU  - Gašić, Uroš M.
AU  - Baošić, Rada
AU  - Joksimović, Kristina
AU  - Pezo, Lato
AU  - Tešić, Živoslav Lj.
AU  - Vovk, Irena
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5505
AB  - This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13–4.94), diastase activity (24.20–41.70 DN), acidity (14.60–29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20–3.90 g/100 g), and moisture content (15.01–19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.
PB  - Frontiers Media S.A.
T2  - Frontiers in Nutrition
T2  - Frontiers in Nutrition
T1  - Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia
VL  - 9
SP  - 941463
DO  - 10.3389/fnut.2022.941463
ER  - 
@article{
author = "Nedić, Nebojša and Nešović, Milica and Radišić, Predrag and Gašić, Uroš M. and Baošić, Rada and Joksimović, Kristina and Pezo, Lato and Tešić, Živoslav Lj. and Vovk, Irena",
year = "2022",
abstract = "This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13–4.94), diastase activity (24.20–41.70 DN), acidity (14.60–29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20–3.90 g/100 g), and moisture content (15.01–19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Nutrition, Frontiers in Nutrition",
title = "Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia",
volume = "9",
pages = "941463",
doi = "10.3389/fnut.2022.941463"
}
Nedić, N., Nešović, M., Radišić, P., Gašić, U. M., Baošić, R., Joksimović, K., Pezo, L., Tešić, Ž. Lj.,& Vovk, I.. (2022). Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia. in Frontiers in Nutrition
Frontiers Media S.A.., 9, 941463.
https://doi.org/10.3389/fnut.2022.941463
Nedić N, Nešović M, Radišić P, Gašić UM, Baošić R, Joksimović K, Pezo L, Tešić ŽL, Vovk I. Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia. in Frontiers in Nutrition. 2022;9:941463.
doi:10.3389/fnut.2022.941463 .
Nedić, Nebojša, Nešović, Milica, Radišić, Predrag, Gašić, Uroš M., Baošić, Rada, Joksimović, Kristina, Pezo, Lato, Tešić, Živoslav Lj., Vovk, Irena, "Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia" in Frontiers in Nutrition, 9 (2022):941463,
https://doi.org/10.3389/fnut.2022.941463 . .
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Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species

Dabić Zagorac, Dragana; Fotirić-Akšić, Milica M.; Glavnik, Vesna; Gašić, Uroš M.; Vovk, Irena; Tešić, Živoslav Lj.; Natić, Maja

(2020)

TY  - JOUR
AU  - Dabić Zagorac, Dragana
AU  - Fotirić-Akšić, Milica M.
AU  - Glavnik, Vesna
AU  - Gašić, Uroš M.
AU  - Vovk, Irena
AU  - Tešić, Živoslav Lj.
AU  - Natić, Maja
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4004
AB  - The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components’ scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.
T2  - Journal of Separation Science
T1  - Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species
VL  - 43
IS  - 8
SP  - 1431
EP  - 1439
DO  - 10.1002/jssc.201901271
ER  - 
@article{
author = "Dabić Zagorac, Dragana and Fotirić-Akšić, Milica M. and Glavnik, Vesna and Gašić, Uroš M. and Vovk, Irena and Tešić, Živoslav Lj. and Natić, Maja",
year = "2020",
abstract = "The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components’ scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.",
journal = "Journal of Separation Science",
title = "Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species",
volume = "43",
number = "8",
pages = "1431-1439",
doi = "10.1002/jssc.201901271"
}
Dabić Zagorac, D., Fotirić-Akšić, M. M., Glavnik, V., Gašić, U. M., Vovk, I., Tešić, Ž. Lj.,& Natić, M.. (2020). Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species. in Journal of Separation Science, 43(8), 1431-1439.
https://doi.org/10.1002/jssc.201901271
Dabić Zagorac D, Fotirić-Akšić MM, Glavnik V, Gašić UM, Vovk I, Tešić ŽL, Natić M. Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species. in Journal of Separation Science. 2020;43(8):1431-1439.
doi:10.1002/jssc.201901271 .
Dabić Zagorac, Dragana, Fotirić-Akšić, Milica M., Glavnik, Vesna, Gašić, Uroš M., Vovk, Irena, Tešić, Živoslav Lj., Natić, Maja, "Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species" in Journal of Separation Science, 43, no. 8 (2020):1431-1439,
https://doi.org/10.1002/jssc.201901271 . .
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8

Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen

Mosić, Mirjana D.; Trifković, Jelena; Vovk, Irena; Gašić, Uroš M.; Tešić, Živoslav Lj.; Šikoparija, Branko; Milojković-Opsenica, Dušanka

(MDPI AG, 2019)

TY  - JOUR
AU  - Mosić, Mirjana D.
AU  - Trifković, Jelena
AU  - Vovk, Irena
AU  - Gašić, Uroš M.
AU  - Tešić, Živoslav Lj.
AU  - Šikoparija, Branko
AU  - Milojković-Opsenica, Dušanka
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3774
PB  - MDPI AG
T2  - Biomolecules
T1  - Phenolic Composition Influences the
Health-Promoting Potential of Bee-Pollen
VL  - 9
IS  - 12
DO  - 10.3390/biom9120783
ER  - 
@article{
author = "Mosić, Mirjana D. and Trifković, Jelena and Vovk, Irena and Gašić, Uroš M. and Tešić, Živoslav Lj. and Šikoparija, Branko and Milojković-Opsenica, Dušanka",
year = "2019",
publisher = "MDPI AG",
journal = "Biomolecules",
title = "Phenolic Composition Influences the
Health-Promoting Potential of Bee-Pollen",
volume = "9",
number = "12",
doi = "10.3390/biom9120783"
}
Mosić, M. D., Trifković, J., Vovk, I., Gašić, U. M., Tešić, Ž. Lj., Šikoparija, B.,& Milojković-Opsenica, D.. (2019). Phenolic Composition Influences the
Health-Promoting Potential of Bee-Pollen. in Biomolecules
MDPI AG., 9(12).
https://doi.org/10.3390/biom9120783
Mosić MD, Trifković J, Vovk I, Gašić UM, Tešić ŽL, Šikoparija B, Milojković-Opsenica D. Phenolic Composition Influences the
Health-Promoting Potential of Bee-Pollen. in Biomolecules. 2019;9(12).
doi:10.3390/biom9120783 .
Mosić, Mirjana D., Trifković, Jelena, Vovk, Irena, Gašić, Uroš M., Tešić, Živoslav Lj., Šikoparija, Branko, Milojković-Opsenica, Dušanka, "Phenolic Composition Influences the
Health-Promoting Potential of Bee-Pollen" in Biomolecules, 9, no. 12 (2019),
https://doi.org/10.3390/biom9120783 . .
1
36
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Supplementary data for article: Mosić, M.; Trifković, J.; Vovk, I.; Gašić, U.; Tešić, Ž.; Šikoparija, B.; Milojković-Opsenica, D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen. Biomolecules 2019, 9 (12). https://doi.org/10.3390/biom9120783

Mosić, Mirjana D.; Trifković, Jelena; Vovk, Irena; Gašić, Uroš M.; Tešić, Živoslav Lj.; Šikoparija, Branko; Milojković-Opsenica, Dušanka

(MDPI AG, 2019)

TY  - DATA
AU  - Mosić, Mirjana D.
AU  - Trifković, Jelena
AU  - Vovk, Irena
AU  - Gašić, Uroš M.
AU  - Tešić, Živoslav Lj.
AU  - Šikoparija, Branko
AU  - Milojković-Opsenica, Dušanka
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3775
PB  - MDPI AG
T2  - Biomolecules
T1  - Supplementary data for article:           Mosić, M.; Trifković, J.; Vovk, I.; Gašić, U.; Tešić, Ž.; Šikoparija, B.; Milojković-Opsenica, D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen. Biomolecules 2019, 9 (12). https://doi.org/10.3390/biom9120783
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3775
ER  - 
@misc{
author = "Mosić, Mirjana D. and Trifković, Jelena and Vovk, Irena and Gašić, Uroš M. and Tešić, Živoslav Lj. and Šikoparija, Branko and Milojković-Opsenica, Dušanka",
year = "2019",
publisher = "MDPI AG",
journal = "Biomolecules",
title = "Supplementary data for article:           Mosić, M.; Trifković, J.; Vovk, I.; Gašić, U.; Tešić, Ž.; Šikoparija, B.; Milojković-Opsenica, D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen. Biomolecules 2019, 9 (12). https://doi.org/10.3390/biom9120783",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3775"
}
Mosić, M. D., Trifković, J., Vovk, I., Gašić, U. M., Tešić, Ž. Lj., Šikoparija, B.,& Milojković-Opsenica, D.. (2019). Supplementary data for article:           Mosić, M.; Trifković, J.; Vovk, I.; Gašić, U.; Tešić, Ž.; Šikoparija, B.; Milojković-Opsenica, D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen. Biomolecules 2019, 9 (12). https://doi.org/10.3390/biom9120783. in Biomolecules
MDPI AG..
https://hdl.handle.net/21.15107/rcub_cherry_3775
Mosić MD, Trifković J, Vovk I, Gašić UM, Tešić ŽL, Šikoparija B, Milojković-Opsenica D. Supplementary data for article:           Mosić, M.; Trifković, J.; Vovk, I.; Gašić, U.; Tešić, Ž.; Šikoparija, B.; Milojković-Opsenica, D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen. Biomolecules 2019, 9 (12). https://doi.org/10.3390/biom9120783. in Biomolecules. 2019;.
https://hdl.handle.net/21.15107/rcub_cherry_3775 .
Mosić, Mirjana D., Trifković, Jelena, Vovk, Irena, Gašić, Uroš M., Tešić, Živoslav Lj., Šikoparija, Branko, Milojković-Opsenica, Dušanka, "Supplementary data for article:           Mosić, M.; Trifković, J.; Vovk, I.; Gašić, U.; Tešić, Ž.; Šikoparija, B.; Milojković-Opsenica, D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen. Biomolecules 2019, 9 (12). https://doi.org/10.3390/biom9120783" in Biomolecules (2019),
https://hdl.handle.net/21.15107/rcub_cherry_3775 .

Supplementary data for article: Ristivojević, P.; Trifković, J.; Vovk, I.; Milojković-Opsenica, D. Comparative Study of Different Approaches for Multivariate Image Analysis in HPTLC Fingerprinting of Natural Products Such as Plant Resin. Talanta 2017, 162, 72–79. https://doi.org/10.1016/j.talanta.2016.10.023

Ristivojević, Petar; Trifković, Jelena; Vovk, Irena; Milojković-Opsenica, Dušanka

(Elsevier Science Bv, Amsterdam, 2017)

TY  - DATA
AU  - Ristivojević, Petar
AU  - Trifković, Jelena
AU  - Vovk, Irena
AU  - Milojković-Opsenica, Dušanka
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3065
PB  - Elsevier Science Bv, Amsterdam
T2  - Talanta
T1  - Supplementary data for article:          Ristivojević, P.; Trifković, J.; Vovk, I.; Milojković-Opsenica, D. Comparative Study of Different Approaches for Multivariate Image Analysis in HPTLC Fingerprinting of Natural Products Such as Plant Resin. Talanta 2017, 162, 72–79. https://doi.org/10.1016/j.talanta.2016.10.023
DO  - 10.1016/j.talanta.2016.10.023
ER  - 
@misc{
author = "Ristivojević, Petar and Trifković, Jelena and Vovk, Irena and Milojković-Opsenica, Dušanka",
year = "2017",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Talanta",
title = "Supplementary data for article:          Ristivojević, P.; Trifković, J.; Vovk, I.; Milojković-Opsenica, D. Comparative Study of Different Approaches for Multivariate Image Analysis in HPTLC Fingerprinting of Natural Products Such as Plant Resin. Talanta 2017, 162, 72–79. https://doi.org/10.1016/j.talanta.2016.10.023",
doi = "10.1016/j.talanta.2016.10.023"
}
Ristivojević, P., Trifković, J., Vovk, I.,& Milojković-Opsenica, D.. (2017). Supplementary data for article:          Ristivojević, P.; Trifković, J.; Vovk, I.; Milojković-Opsenica, D. Comparative Study of Different Approaches for Multivariate Image Analysis in HPTLC Fingerprinting of Natural Products Such as Plant Resin. Talanta 2017, 162, 72–79. https://doi.org/10.1016/j.talanta.2016.10.023. in Talanta
Elsevier Science Bv, Amsterdam..
https://doi.org/10.1016/j.talanta.2016.10.023
Ristivojević P, Trifković J, Vovk I, Milojković-Opsenica D. Supplementary data for article:          Ristivojević, P.; Trifković, J.; Vovk, I.; Milojković-Opsenica, D. Comparative Study of Different Approaches for Multivariate Image Analysis in HPTLC Fingerprinting of Natural Products Such as Plant Resin. Talanta 2017, 162, 72–79. https://doi.org/10.1016/j.talanta.2016.10.023. in Talanta. 2017;.
doi:10.1016/j.talanta.2016.10.023 .
Ristivojević, Petar, Trifković, Jelena, Vovk, Irena, Milojković-Opsenica, Dušanka, "Supplementary data for article:          Ristivojević, P.; Trifković, J.; Vovk, I.; Milojković-Opsenica, D. Comparative Study of Different Approaches for Multivariate Image Analysis in HPTLC Fingerprinting of Natural Products Such as Plant Resin. Talanta 2017, 162, 72–79. https://doi.org/10.1016/j.talanta.2016.10.023" in Talanta (2017),
https://doi.org/10.1016/j.talanta.2016.10.023 . .
48
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Comparative study of different approaches for multivariate image analysis in HPTLC fingerprinting of natural products such as plant resin

Ristivojević, Petar; Trifković, Jelena; Vovk, Irena; Milojković-Opsenica, Dušanka

(Elsevier Science Bv, Amsterdam, 2017)

TY  - JOUR
AU  - Ristivojević, Petar
AU  - Trifković, Jelena
AU  - Vovk, Irena
AU  - Milojković-Opsenica, Dušanka
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2359
AB  - Considering the introduction of phytochemical fingerprint analysis, as a method of screening the complex natural products for the presence of most bioactive compounds, use of chemometric classification methods, application of powerful scanning and image capturing and processing devices and algorithms, advancement in development of novel stationary phases as well as various separation modalities, high-performance thin-layer chromatography (HPTLC) fingerprinting is becoming attractive and fruitful field of separation science. Multivariate image analysis is crucial in the light of proper data acquisition. In a current study, different image processing procedures were studied and compared in detail on the example of HPTLC chromatograms of plant resins. In that sense, obtained variables such as gray intensities of pixels along the solvent front, peak area and mean values of peak were used as input data and compared to obtained best classification models. Important steps in image analysis, baseline removal, denoising, target peak alignment and normalization were pointed out. Numerical data set based on mean value of selected bands and intensities of pixels along the solvent front proved to be the most convenient for planar-chromatographic profiling, although required at least the basic knowledge on image processing methodology, and could be proposed for further investigation in HPLTC fingerprinting.
PB  - Elsevier Science Bv, Amsterdam
T2  - Talanta
T1  - Comparative study of different approaches for multivariate image analysis in HPTLC fingerprinting of natural products such as plant resin
VL  - 162
SP  - 72
EP  - 79
DO  - 10.1016/j.talanta.2016.10.023
ER  - 
@article{
author = "Ristivojević, Petar and Trifković, Jelena and Vovk, Irena and Milojković-Opsenica, Dušanka",
year = "2017",
abstract = "Considering the introduction of phytochemical fingerprint analysis, as a method of screening the complex natural products for the presence of most bioactive compounds, use of chemometric classification methods, application of powerful scanning and image capturing and processing devices and algorithms, advancement in development of novel stationary phases as well as various separation modalities, high-performance thin-layer chromatography (HPTLC) fingerprinting is becoming attractive and fruitful field of separation science. Multivariate image analysis is crucial in the light of proper data acquisition. In a current study, different image processing procedures were studied and compared in detail on the example of HPTLC chromatograms of plant resins. In that sense, obtained variables such as gray intensities of pixels along the solvent front, peak area and mean values of peak were used as input data and compared to obtained best classification models. Important steps in image analysis, baseline removal, denoising, target peak alignment and normalization were pointed out. Numerical data set based on mean value of selected bands and intensities of pixels along the solvent front proved to be the most convenient for planar-chromatographic profiling, although required at least the basic knowledge on image processing methodology, and could be proposed for further investigation in HPLTC fingerprinting.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Talanta",
title = "Comparative study of different approaches for multivariate image analysis in HPTLC fingerprinting of natural products such as plant resin",
volume = "162",
pages = "72-79",
doi = "10.1016/j.talanta.2016.10.023"
}
Ristivojević, P., Trifković, J., Vovk, I.,& Milojković-Opsenica, D.. (2017). Comparative study of different approaches for multivariate image analysis in HPTLC fingerprinting of natural products such as plant resin. in Talanta
Elsevier Science Bv, Amsterdam., 162, 72-79.
https://doi.org/10.1016/j.talanta.2016.10.023
Ristivojević P, Trifković J, Vovk I, Milojković-Opsenica D. Comparative study of different approaches for multivariate image analysis in HPTLC fingerprinting of natural products such as plant resin. in Talanta. 2017;162:72-79.
doi:10.1016/j.talanta.2016.10.023 .
Ristivojević, Petar, Trifković, Jelena, Vovk, Irena, Milojković-Opsenica, Dušanka, "Comparative study of different approaches for multivariate image analysis in HPTLC fingerprinting of natural products such as plant resin" in Talanta, 162 (2017):72-79,
https://doi.org/10.1016/j.talanta.2016.10.023 . .
48
36
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43

Quality assessment of marketed chamomile tea products by a validated HPTLC method combined with multivariate analysis

Guzelmeric, Etil; Ristivojević, Petar; Vovk, Irena; Milojković-Opsenica, Dušanka; Yesilada, Erdem

(Elsevier Science Bv, Amsterdam, 2017)

TY  - JOUR
AU  - Guzelmeric, Etil
AU  - Ristivojević, Petar
AU  - Vovk, Irena
AU  - Milojković-Opsenica, Dušanka
AU  - Yesilada, Erdem
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2358
AB  - Chamomile tea composed of dried flower heads of Matricaria recutita L. (Asteraceae) is one of the most popular single ingredient herbal teas. Tea industries, spice shops or public bazaars are mostly supplied chamomile as a raw material via cultivation or through nature-picking. However, one of the drawbacks of nature-picking is adulteration. This could be either due to false authentication of the plant materials by ingenuous pickers or intentional/unintentional substitution with other flowers resembling to chamomile in appearance during harvesting. Therefore, quality control of raw chamomile materials before marketing should be carefully considered not only by quantification of apigenin 7-O-glucoside (active marker) but also by fingerprinting of chemical composition. This work presents both quantification of apigenin 7-O-glucoside and chemical fingerprinting of commercial chamomile tea products obtained from different food stores and spice shops by a validated HPTLC method. In addition, HPTLC profiles of investigated chamomile tea samples were compared with HPLC method stated in the European Pharmacopoeia and it was found that HPTLC method was superior to HPLC method in the field of adulteration confirmation. Therefore, fingerprint profiles performed on the silica gel 60 NH2 F254S HPTLC plates combined with pattern recognition techniques of these marketed products were comparatively evaluated with wild and cultivar chamomile samples and also chamomile-like species from Asteraceae. Consequently, not chamomile tea bags but crude flowers sold on market were found to be adulterated with other plant materials. (C) 2016 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Quality assessment of marketed chamomile tea products by a validated HPTLC method combined with multivariate analysis
VL  - 132
SP  - 35
EP  - 45
DO  - 10.1016/j.jpba.2016.09.030
ER  - 
@article{
author = "Guzelmeric, Etil and Ristivojević, Petar and Vovk, Irena and Milojković-Opsenica, Dušanka and Yesilada, Erdem",
year = "2017",
abstract = "Chamomile tea composed of dried flower heads of Matricaria recutita L. (Asteraceae) is one of the most popular single ingredient herbal teas. Tea industries, spice shops or public bazaars are mostly supplied chamomile as a raw material via cultivation or through nature-picking. However, one of the drawbacks of nature-picking is adulteration. This could be either due to false authentication of the plant materials by ingenuous pickers or intentional/unintentional substitution with other flowers resembling to chamomile in appearance during harvesting. Therefore, quality control of raw chamomile materials before marketing should be carefully considered not only by quantification of apigenin 7-O-glucoside (active marker) but also by fingerprinting of chemical composition. This work presents both quantification of apigenin 7-O-glucoside and chemical fingerprinting of commercial chamomile tea products obtained from different food stores and spice shops by a validated HPTLC method. In addition, HPTLC profiles of investigated chamomile tea samples were compared with HPLC method stated in the European Pharmacopoeia and it was found that HPTLC method was superior to HPLC method in the field of adulteration confirmation. Therefore, fingerprint profiles performed on the silica gel 60 NH2 F254S HPTLC plates combined with pattern recognition techniques of these marketed products were comparatively evaluated with wild and cultivar chamomile samples and also chamomile-like species from Asteraceae. Consequently, not chamomile tea bags but crude flowers sold on market were found to be adulterated with other plant materials. (C) 2016 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Quality assessment of marketed chamomile tea products by a validated HPTLC method combined with multivariate analysis",
volume = "132",
pages = "35-45",
doi = "10.1016/j.jpba.2016.09.030"
}
Guzelmeric, E., Ristivojević, P., Vovk, I., Milojković-Opsenica, D.,& Yesilada, E.. (2017). Quality assessment of marketed chamomile tea products by a validated HPTLC method combined with multivariate analysis. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science Bv, Amsterdam., 132, 35-45.
https://doi.org/10.1016/j.jpba.2016.09.030
Guzelmeric E, Ristivojević P, Vovk I, Milojković-Opsenica D, Yesilada E. Quality assessment of marketed chamomile tea products by a validated HPTLC method combined with multivariate analysis. in Journal of Pharmaceutical and Biomedical Analysis. 2017;132:35-45.
doi:10.1016/j.jpba.2016.09.030 .
Guzelmeric, Etil, Ristivojević, Petar, Vovk, Irena, Milojković-Opsenica, Dušanka, Yesilada, Erdem, "Quality assessment of marketed chamomile tea products by a validated HPTLC method combined with multivariate analysis" in Journal of Pharmaceutical and Biomedical Analysis, 132 (2017):35-45,
https://doi.org/10.1016/j.jpba.2016.09.030 . .
36
27
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32

Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Trifković, J.; Berić, T.; Vovk, I.; Milojkovič-Opsenica, D.; Stanković, S. Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics. PLoS ONE 2016, 11 (6). https://doi.org/10.1371/journal.pone.0157097

Ristivojević, Petar; Dimkić, Ivica; Trifković, Jelena; Berić, Tanja; Vovk, Irena; Milojković-Opsenica, Dušanka; Stanković, Slaviša

(Public Library Science, San Francisco, 2016)

TY  - DATA
AU  - Ristivojević, Petar
AU  - Dimkić, Ivica
AU  - Trifković, Jelena
AU  - Berić, Tanja
AU  - Vovk, Irena
AU  - Milojković-Opsenica, Dušanka
AU  - Stanković, Slaviša
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3345
PB  - Public Library Science, San Francisco
T2  - PLoS One / Public Library of Science
T1  - Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Trifković, J.; Berić, T.; Vovk, I.; Milojkovič-Opsenica, D.; Stanković, S. Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics. PLoS ONE 2016, 11 (6). https://doi.org/10.1371/journal.pone.0157097
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3345
ER  - 
@misc{
author = "Ristivojević, Petar and Dimkić, Ivica and Trifković, Jelena and Berić, Tanja and Vovk, Irena and Milojković-Opsenica, Dušanka and Stanković, Slaviša",
year = "2016",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One / Public Library of Science",
title = "Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Trifković, J.; Berić, T.; Vovk, I.; Milojkovič-Opsenica, D.; Stanković, S. Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics. PLoS ONE 2016, 11 (6). https://doi.org/10.1371/journal.pone.0157097",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3345"
}
Ristivojević, P., Dimkić, I., Trifković, J., Berić, T., Vovk, I., Milojković-Opsenica, D.,& Stanković, S.. (2016). Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Trifković, J.; Berić, T.; Vovk, I.; Milojkovič-Opsenica, D.; Stanković, S. Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics. PLoS ONE 2016, 11 (6). https://doi.org/10.1371/journal.pone.0157097. in PLoS One / Public Library of Science
Public Library Science, San Francisco..
https://hdl.handle.net/21.15107/rcub_cherry_3345
Ristivojević P, Dimkić I, Trifković J, Berić T, Vovk I, Milojković-Opsenica D, Stanković S. Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Trifković, J.; Berić, T.; Vovk, I.; Milojkovič-Opsenica, D.; Stanković, S. Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics. PLoS ONE 2016, 11 (6). https://doi.org/10.1371/journal.pone.0157097. in PLoS One / Public Library of Science. 2016;.
https://hdl.handle.net/21.15107/rcub_cherry_3345 .
Ristivojević, Petar, Dimkić, Ivica, Trifković, Jelena, Berić, Tanja, Vovk, Irena, Milojković-Opsenica, Dušanka, Stanković, Slaviša, "Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Trifković, J.; Berić, T.; Vovk, I.; Milojkovič-Opsenica, D.; Stanković, S. Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics. PLoS ONE 2016, 11 (6). https://doi.org/10.1371/journal.pone.0157097" in PLoS One / Public Library of Science (2016),
https://hdl.handle.net/21.15107/rcub_cherry_3345 .

Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics

Ristivojević, Petar; Dimkić, Ivica; Trifković, Jelena; Berić, Tanja; Vovk, Irena; Milojković-Opsenica, Dušanka; Stanković, Slaviša

(Public Library Science, San Francisco, 2016)

TY  - JOUR
AU  - Ristivojević, Petar
AU  - Dimkić, Ivica
AU  - Trifković, Jelena
AU  - Berić, Tanja
AU  - Vovk, Irena
AU  - Milojković-Opsenica, Dušanka
AU  - Stanković, Slaviša
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2261
AB  - New information has come to light about the biological activity of propolis and the quality of natural products which requires a rapid and reliable assessment method such as High Performance Thin-Layer Chromatography (HPTLC) fingerprinting. This study investigates chromatographic and chemometric approaches for determining the antimicrobial activity of propolis of Serbian origin against various bacterial species. A linear multivariate calibration technique, using Partial Least Squares, was used to extract the relevant information from the chromatographic fingerprints, i.e. to indicate peaks which represent phenolic compounds that are potentially responsible for the antimicrobial capacity of the samples. In addition, direct bioautography was performed to localize the antibacterial activity on chromatograms. The biological activity of the propolis samples against various bacterial species was determined by a minimum inhibitory concentration assay, confirming their affiliation with the European poplar type of propolis and revealing the existence of two types (blue and orange) according to botanical origin. The strongest antibacterial activity was exhibited by sample 26 against Staphylococcus aureus, with a MIC value of 0.5 mg/mL, and Listeria monocytogenes, with a MIC as low as 0.1 mg/mL, which was also the lowest effective concentration observed in our study. Generally, the orange type of propolis shows higher antimicrobial activity compared to the blue type. PLS modelling was performed on the HPTLC data set and the resulting models might qualitatively indicate compounds that play an important role in the activity exhibited by the propolis samples. The most relevant peaks influencing the antimicrobial activity of propolis against all bacterial strains were phenolic compounds at RF values of 0.37, 0.40, 0.45, 0.51, 0.60 and 0.70. The knowledge gained through this study could be important for attributing the antimicrobial activity of propolis to specific chemical compounds, as well as the verification of HPTLC fingerprinting as a reliable method for the identification of compounds that are potentially responsible for antimicrobial activity. This is the first report on the activity of Serbian propolis as determined by several combined methods, including the modelling of antimicrobial activity by HPTLC fingerprinting.
PB  - Public Library Science, San Francisco
T2  - PLoS One / Public Library of Science
T1  - Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics
VL  - 11
IS  - 6
DO  - 10.1371/journal.pone.0157097
ER  - 
@article{
author = "Ristivojević, Petar and Dimkić, Ivica and Trifković, Jelena and Berić, Tanja and Vovk, Irena and Milojković-Opsenica, Dušanka and Stanković, Slaviša",
year = "2016",
abstract = "New information has come to light about the biological activity of propolis and the quality of natural products which requires a rapid and reliable assessment method such as High Performance Thin-Layer Chromatography (HPTLC) fingerprinting. This study investigates chromatographic and chemometric approaches for determining the antimicrobial activity of propolis of Serbian origin against various bacterial species. A linear multivariate calibration technique, using Partial Least Squares, was used to extract the relevant information from the chromatographic fingerprints, i.e. to indicate peaks which represent phenolic compounds that are potentially responsible for the antimicrobial capacity of the samples. In addition, direct bioautography was performed to localize the antibacterial activity on chromatograms. The biological activity of the propolis samples against various bacterial species was determined by a minimum inhibitory concentration assay, confirming their affiliation with the European poplar type of propolis and revealing the existence of two types (blue and orange) according to botanical origin. The strongest antibacterial activity was exhibited by sample 26 against Staphylococcus aureus, with a MIC value of 0.5 mg/mL, and Listeria monocytogenes, with a MIC as low as 0.1 mg/mL, which was also the lowest effective concentration observed in our study. Generally, the orange type of propolis shows higher antimicrobial activity compared to the blue type. PLS modelling was performed on the HPTLC data set and the resulting models might qualitatively indicate compounds that play an important role in the activity exhibited by the propolis samples. The most relevant peaks influencing the antimicrobial activity of propolis against all bacterial strains were phenolic compounds at RF values of 0.37, 0.40, 0.45, 0.51, 0.60 and 0.70. The knowledge gained through this study could be important for attributing the antimicrobial activity of propolis to specific chemical compounds, as well as the verification of HPTLC fingerprinting as a reliable method for the identification of compounds that are potentially responsible for antimicrobial activity. This is the first report on the activity of Serbian propolis as determined by several combined methods, including the modelling of antimicrobial activity by HPTLC fingerprinting.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One / Public Library of Science",
title = "Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics",
volume = "11",
number = "6",
doi = "10.1371/journal.pone.0157097"
}
Ristivojević, P., Dimkić, I., Trifković, J., Berić, T., Vovk, I., Milojković-Opsenica, D.,& Stanković, S.. (2016). Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics. in PLoS One / Public Library of Science
Public Library Science, San Francisco., 11(6).
https://doi.org/10.1371/journal.pone.0157097
Ristivojević P, Dimkić I, Trifković J, Berić T, Vovk I, Milojković-Opsenica D, Stanković S. Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics. in PLoS One / Public Library of Science. 2016;11(6).
doi:10.1371/journal.pone.0157097 .
Ristivojević, Petar, Dimkić, Ivica, Trifković, Jelena, Berić, Tanja, Vovk, Irena, Milojković-Opsenica, Dušanka, Stanković, Slaviša, "Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics" in PLoS One / Public Library of Science, 11, no. 6 (2016),
https://doi.org/10.1371/journal.pone.0157097 . .
1
67
45
70
57

TLC Fingerprinting and Pattern Recognition Methods in the Assessment of Authenticity of Poplar-Type Propolis

Milojković-Opsenica, Dušanka; Ristivojević, Petar; Trifković, Jelena; Vovk, Irena; Lušić, Dražen; Tešić, Živoslav Lj.

(Oxford Univ Press Inc, Cary, 2016)

TY  - JOUR
AU  - Milojković-Opsenica, Dušanka
AU  - Ristivojević, Petar
AU  - Trifković, Jelena
AU  - Vovk, Irena
AU  - Lušić, Dražen
AU  - Tešić, Živoslav Lj.
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2360
AB  - Propolis is a "natural" remedy with prominent biological activity, which is used as dietary supplement. In the absence of clinical studies that would substantiate these claims, information on the biological activity of propolis is valuable. This study comprises chromatographic, image processing and chemometric approach for phenolic profiling of Serbian, Croatian and Slovenian propolis test solutions. Modern thin-layer chromatography equipment in combination with software for image processing was applied for fingerprinting and data acquisition, whereas the principal component analysis was used as pattern recognition method. Characterization of phenolic profile was performed along with the determination of the botanical and geographical origin of propolis. High-performance thin-layer chromatograms reveal that Central and Southeastern European propolis samples are rich in flavonoids. In addition, phenolic compounds proved to be suitable markers for the determination of European propolis authenticity.
PB  - Oxford Univ Press Inc, Cary
T2  - Journal of Chromatographic Science
T1  - TLC Fingerprinting and Pattern Recognition Methods in the Assessment of Authenticity of Poplar-Type Propolis
VL  - 54
IS  - 7
SP  - 1077
EP  - 1083
DO  - 10.1093/chromsci/bmw024
ER  - 
@article{
author = "Milojković-Opsenica, Dušanka and Ristivojević, Petar and Trifković, Jelena and Vovk, Irena and Lušić, Dražen and Tešić, Živoslav Lj.",
year = "2016",
abstract = "Propolis is a "natural" remedy with prominent biological activity, which is used as dietary supplement. In the absence of clinical studies that would substantiate these claims, information on the biological activity of propolis is valuable. This study comprises chromatographic, image processing and chemometric approach for phenolic profiling of Serbian, Croatian and Slovenian propolis test solutions. Modern thin-layer chromatography equipment in combination with software for image processing was applied for fingerprinting and data acquisition, whereas the principal component analysis was used as pattern recognition method. Characterization of phenolic profile was performed along with the determination of the botanical and geographical origin of propolis. High-performance thin-layer chromatograms reveal that Central and Southeastern European propolis samples are rich in flavonoids. In addition, phenolic compounds proved to be suitable markers for the determination of European propolis authenticity.",
publisher = "Oxford Univ Press Inc, Cary",
journal = "Journal of Chromatographic Science",
title = "TLC Fingerprinting and Pattern Recognition Methods in the Assessment of Authenticity of Poplar-Type Propolis",
volume = "54",
number = "7",
pages = "1077-1083",
doi = "10.1093/chromsci/bmw024"
}
Milojković-Opsenica, D., Ristivojević, P., Trifković, J., Vovk, I., Lušić, D.,& Tešić, Ž. Lj.. (2016). TLC Fingerprinting and Pattern Recognition Methods in the Assessment of Authenticity of Poplar-Type Propolis. in Journal of Chromatographic Science
Oxford Univ Press Inc, Cary., 54(7), 1077-1083.
https://doi.org/10.1093/chromsci/bmw024
Milojković-Opsenica D, Ristivojević P, Trifković J, Vovk I, Lušić D, Tešić ŽL. TLC Fingerprinting and Pattern Recognition Methods in the Assessment of Authenticity of Poplar-Type Propolis. in Journal of Chromatographic Science. 2016;54(7):1077-1083.
doi:10.1093/chromsci/bmw024 .
Milojković-Opsenica, Dušanka, Ristivojević, Petar, Trifković, Jelena, Vovk, Irena, Lušić, Dražen, Tešić, Živoslav Lj., "TLC Fingerprinting and Pattern Recognition Methods in the Assessment of Authenticity of Poplar-Type Propolis" in Journal of Chromatographic Science, 54, no. 7 (2016):1077-1083,
https://doi.org/10.1093/chromsci/bmw024 . .
47
29
44
42

Pattern recognition methods and multivariate image analysis in HPTLC fingerprinting of propolis extracts

Ristivojević, Petar; Andrić, Filip; Trifković, Jelena; Vovk, Irena; Stanisavljević, Ljubiša; Tešić, Živoslav Lj.; Milojković-Opsenica, Dušanka

(Wiley-Blackwell, Hoboken, 2014)

TY  - JOUR
AU  - Ristivojević, Petar
AU  - Andrić, Filip
AU  - Trifković, Jelena
AU  - Vovk, Irena
AU  - Stanisavljević, Ljubiša
AU  - Tešić, Živoslav Lj.
AU  - Milojković-Opsenica, Dušanka
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1521
AB  - High-performance thin-layer chromatography (HPTLC) combined with image analysis and pattern recognition methods were used for fingerprinting and classification of 52 propolis samples collected from Serbia and one sample from Croatia. Modern thin-layer chromatography equipment in combination with software for image processing and warping was applied for fingerprinting and data acquisition. The three mostly used chemometric techniques for classification, principal component analysis, cluster analysis and partial least square-discriminant analysis, in combination with simple and fast HPTLC method for fingerprint analysis of propolis, were performed in order to favor and encourage their use in planar chromatography. HPTLC fingerprint analysis of propolis was for the first time performed on amino silica plates. All studied propolis samples have been classified in two major types, orange and blue, supporting the idea of existence of two types of European propolis. Signals at specific R-F values responsible for classification of studied extracts have also been isolated and underlying compounds targeted for further investigation. Copyright (c) 2014 John Wiley & Sons, Ltd. High-performance thin-layer chromatography combined with image analysis and pattern recognition methods were used for fingerprinting and classification of 53 propolis samples. High-performance thin-layer chromatography analysis of propolis was for the first time performed on amino silica plates. All studied propolis samples have been classified in two major types, orange and blue, supporting the idea of existence of two varieties of European propolis. Signals at specific R-F values responsible for classification of studied extracts have also been isolated and underlying compounds targeted for further investigation.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Chemometrics
T1  - Pattern recognition methods and multivariate image analysis in HPTLC fingerprinting of propolis extracts
VL  - 28
IS  - 4
SP  - 301
EP  - 310
DO  - 10.1002/cem.2592
ER  - 
@article{
author = "Ristivojević, Petar and Andrić, Filip and Trifković, Jelena and Vovk, Irena and Stanisavljević, Ljubiša and Tešić, Živoslav Lj. and Milojković-Opsenica, Dušanka",
year = "2014",
abstract = "High-performance thin-layer chromatography (HPTLC) combined with image analysis and pattern recognition methods were used for fingerprinting and classification of 52 propolis samples collected from Serbia and one sample from Croatia. Modern thin-layer chromatography equipment in combination with software for image processing and warping was applied for fingerprinting and data acquisition. The three mostly used chemometric techniques for classification, principal component analysis, cluster analysis and partial least square-discriminant analysis, in combination with simple and fast HPTLC method for fingerprint analysis of propolis, were performed in order to favor and encourage their use in planar chromatography. HPTLC fingerprint analysis of propolis was for the first time performed on amino silica plates. All studied propolis samples have been classified in two major types, orange and blue, supporting the idea of existence of two types of European propolis. Signals at specific R-F values responsible for classification of studied extracts have also been isolated and underlying compounds targeted for further investigation. Copyright (c) 2014 John Wiley & Sons, Ltd. High-performance thin-layer chromatography combined with image analysis and pattern recognition methods were used for fingerprinting and classification of 53 propolis samples. High-performance thin-layer chromatography analysis of propolis was for the first time performed on amino silica plates. All studied propolis samples have been classified in two major types, orange and blue, supporting the idea of existence of two varieties of European propolis. Signals at specific R-F values responsible for classification of studied extracts have also been isolated and underlying compounds targeted for further investigation.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Chemometrics",
title = "Pattern recognition methods and multivariate image analysis in HPTLC fingerprinting of propolis extracts",
volume = "28",
number = "4",
pages = "301-310",
doi = "10.1002/cem.2592"
}
Ristivojević, P., Andrić, F., Trifković, J., Vovk, I., Stanisavljević, L., Tešić, Ž. Lj.,& Milojković-Opsenica, D.. (2014). Pattern recognition methods and multivariate image analysis in HPTLC fingerprinting of propolis extracts. in Journal of Chemometrics
Wiley-Blackwell, Hoboken., 28(4), 301-310.
https://doi.org/10.1002/cem.2592
Ristivojević P, Andrić F, Trifković J, Vovk I, Stanisavljević L, Tešić ŽL, Milojković-Opsenica D. Pattern recognition methods and multivariate image analysis in HPTLC fingerprinting of propolis extracts. in Journal of Chemometrics. 2014;28(4):301-310.
doi:10.1002/cem.2592 .
Ristivojević, Petar, Andrić, Filip, Trifković, Jelena, Vovk, Irena, Stanisavljević, Ljubiša, Tešić, Živoslav Lj., Milojković-Opsenica, Dušanka, "Pattern recognition methods and multivariate image analysis in HPTLC fingerprinting of propolis extracts" in Journal of Chemometrics, 28, no. 4 (2014):301-310,
https://doi.org/10.1002/cem.2592 . .
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