TY  - JOUR
AU  - Popović, Milica M.
AU  - de Marco, Ario
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2138
AB  - Extracellular vesicles (EVs) play a pivotal role in cell to cell signalling in both physiological and pathological conditions. Based on their biogenesis, three main classes of EVs are recognized: exosomes, microvesicles and apoptotic bodies. Exosomes are cell-derived vesicles (EVs) present in many body fluids (blood, urine, milk, cerebrospinal fluid) ranging in size from 30 to 150 nm. Due to their involvement in numerous physiological and pathological events, cell derived exosomes in bodily fluids represent a unique source of clinically relevant and non-invasive biomarkers. Since biomolecule content present in exosomes reflects the state of the parent cell, exosome analysis and characterization may provide valuable information about the presence of aberrant processes in the cells from which they originated. Because of the large and heterogeneous scientific community working with exosomes, several purification strategies have been applied so far, which yield EV fractions largely differing for quantity and quality. Most of the present exosome isolation approaches based on ultracentrifugation (UC), ultrafiltration (UF) or precipitation, are inefficient and hard to standardize, thereby creating low reproducibility in sample quality and potentially misleading results because highly sensitivity downstream analytical techniques, such as mass spectrometry, can detect even minute traces of co-isolated contaminants. Furthermore, loss of certain exosomal fractions during purification process or damage of exosomal membrane integrity can also alter final protein and RNA profiles. As a consequence, there is a strong interest in consensus principles for the exosome purification and the search for reliable methods for selective isolation of EV sub-populations. In the present manuscript, we critically overview the most commonly used techniques used for exosome preparation such as ultracentrifugation, size-based isolation methods, precipitation and immunoaffinity (IA) and their respective applicability for purification of exosomes from clinically relevant samples.
PB  - Ame Publ Co, Shatin
T2  - TRANSLATIONAL CANCER RESEARCH
T1  - Canonical and selective approaches in exosome purification and their implications for diagnostic accuracy
VL  - 7
DO  - 10.21037/tcr.2017.08.44
ER  - 

@article{
author = "Popović, Milica M. and de Marco, Ario",
year = "2018",
abstract = "Extracellular vesicles (EVs) play a pivotal role in cell to cell signalling in both physiological and pathological conditions. Based on their biogenesis, three main classes of EVs are recognized: exosomes, microvesicles and apoptotic bodies. Exosomes are cell-derived vesicles (EVs) present in many body fluids (blood, urine, milk, cerebrospinal fluid) ranging in size from 30 to 150 nm. Due to their involvement in numerous physiological and pathological events, cell derived exosomes in bodily fluids represent a unique source of clinically relevant and non-invasive biomarkers. Since biomolecule content present in exosomes reflects the state of the parent cell, exosome analysis and characterization may provide valuable information about the presence of aberrant processes in the cells from which they originated. Because of the large and heterogeneous scientific community working with exosomes, several purification strategies have been applied so far, which yield EV fractions largely differing for quantity and quality. Most of the present exosome isolation approaches based on ultracentrifugation (UC), ultrafiltration (UF) or precipitation, are inefficient and hard to standardize, thereby creating low reproducibility in sample quality and potentially misleading results because highly sensitivity downstream analytical techniques, such as mass spectrometry, can detect even minute traces of co-isolated contaminants. Furthermore, loss of certain exosomal fractions during purification process or damage of exosomal membrane integrity can also alter final protein and RNA profiles. As a consequence, there is a strong interest in consensus principles for the exosome purification and the search for reliable methods for selective isolation of EV sub-populations. In the present manuscript, we critically overview the most commonly used techniques used for exosome preparation such as ultracentrifugation, size-based isolation methods, precipitation and immunoaffinity (IA) and their respective applicability for purification of exosomes from clinically relevant samples.",
publisher = "Ame Publ Co, Shatin",
journal = "TRANSLATIONAL CANCER RESEARCH",
title = "Canonical and selective approaches in exosome purification and their implications for diagnostic accuracy",
volume = "7",
doi = "10.21037/tcr.2017.08.44"
}

Popović, M. M.,& de Marco, A.. (2018). Canonical and selective approaches in exosome purification and their implications for diagnostic accuracy. in TRANSLATIONAL CANCER RESEARCH
Ame Publ Co, Shatin., 7.
https://doi.org/10.21037/tcr.2017.08.44

Popović MM, de Marco A. Canonical and selective approaches in exosome purification and their implications for diagnostic accuracy. in TRANSLATIONAL CANCER RESEARCH. 2018;7.
doi:10.21037/tcr.2017.08.44 .

Popović, Milica M., de Marco, Ario, "Canonical and selective approaches in exosome purification and their implications for diagnostic accuracy" in TRANSLATIONAL CANCER RESEARCH, 7 (2018),
https://doi.org/10.21037/tcr.2017.08.44 . .

TY  - JOUR
AU  - Popović, Milica M.
AU  - Gregori, Marco
AU  - Vodnik, Dominik
AU  - Ferlan, Mitja
AU  - Mrak, Tanja
AU  - Straus, Ines
AU  - McDowell, Nathan G.
AU  - Kraigher, Hojka
AU  - de Marco, Ario
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2546
AB  - Drought is an environmental stress that impacts plant productivity. Projections show both an increase in intense rain events and a reduction in the number of rain days, conditions that leads to increased risk of drought. Consequently, the identification of molecular biomarkers suitable for evaluating the impact of water deprivation conditions on forest plant seedlings is of significant value for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of drought stress coupled with variable soil temperature in European beech (Fagus sylvatica L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the specific activities and isoform profile of superoxide dismutases and glutathione peroxidases together with the variation of phenolic compounds enable discrimination between seedlings with different degrees of photosynthetic activity. This approach represents a promising platform for the assessment of drought stress in forest trees and could serve for enhancing selection and breeding practices, allowing for plants that are more tolerant of abiotic stress.
PB  - Canadian Science Publishing, Nrc Research Press, Ottawa
T2  - Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere
T1  - Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica)
VL  - 47
IS  - 11
SP  - 1517
EP  - 1526
DO  - 10.1139/cjfr-2016-0530
ER  - 

@article{
author = "Popović, Milica M. and Gregori, Marco and Vodnik, Dominik and Ferlan, Mitja and Mrak, Tanja and Straus, Ines and McDowell, Nathan G. and Kraigher, Hojka and de Marco, Ario",
year = "2017",
abstract = "Drought is an environmental stress that impacts plant productivity. Projections show both an increase in intense rain events and a reduction in the number of rain days, conditions that leads to increased risk of drought. Consequently, the identification of molecular biomarkers suitable for evaluating the impact of water deprivation conditions on forest plant seedlings is of significant value for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of drought stress coupled with variable soil temperature in European beech (Fagus sylvatica L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the specific activities and isoform profile of superoxide dismutases and glutathione peroxidases together with the variation of phenolic compounds enable discrimination between seedlings with different degrees of photosynthetic activity. This approach represents a promising platform for the assessment of drought stress in forest trees and could serve for enhancing selection and breeding practices, allowing for plants that are more tolerant of abiotic stress.",
publisher = "Canadian Science Publishing, Nrc Research Press, Ottawa",
journal = "Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere",
title = "Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica)",
volume = "47",
number = "11",
pages = "1517-1526",
doi = "10.1139/cjfr-2016-0530"
}

Popović, M. M., Gregori, M., Vodnik, D., Ferlan, M., Mrak, T., Straus, I., McDowell, N. G., Kraigher, H.,& de Marco, A.. (2017). Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica). in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere
Canadian Science Publishing, Nrc Research Press, Ottawa., 47(11), 1517-1526.
https://doi.org/10.1139/cjfr-2016-0530

Popović MM, Gregori M, Vodnik D, Ferlan M, Mrak T, Straus I, McDowell NG, Kraigher H, de Marco A. Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica). in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere. 2017;47(11):1517-1526.
doi:10.1139/cjfr-2016-0530 .

Popović, Milica M., Gregori, Marco, Vodnik, Dominik, Ferlan, Mitja, Mrak, Tanja, Straus, Ines, McDowell, Nathan G., Kraigher, Hojka, de Marco, Ario, "Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica)" in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere, 47, no. 11 (2017):1517-1526,
https://doi.org/10.1139/cjfr-2016-0530 . .

TY  - JOUR
AU  - Popović, Milica M.
AU  - Gregori, Marco
AU  - Vodnik, Dominik
AU  - Ferlan, Mitja
AU  - Mrak, Tanja
AU  - Straus, Ines
AU  - McDowell, Nathan G.
AU  - Kraigher, Hojka
AU  - de Marco, Ario
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2973
AB  - Drought is an environmental stress that impacts plant productivity. Projections show both an increase in intense rain events and a reduction in the number of rain days, conditions that leads to increased risk of drought. Consequently, the identification of molecular biomarkers suitable for evaluating the impact of water deprivation conditions on forest plant seedlings is of significant value for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of drought stress coupled with variable soil temperature in European beech (Fagus sylvatica L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the specific activities and isoform profile of superoxide dismutases and glutathione peroxidases together with the variation of phenolic compounds enable discrimination between seedlings with different degrees of photosynthetic activity. This approach represents a promising platform for the assessment of drought stress in forest trees and could serve for enhancing selection and breeding practices, allowing for plants that are more tolerant of abiotic stress.
PB  - Canadian Science Publishing, Nrc Research Press, Ottawa
T2  - Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere
T1  - Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica)
VL  - 47
IS  - 11
SP  - 1517
EP  - 1526
DO  - 10.1139/cjfr-2016-0530
ER  - 

@article{
author = "Popović, Milica M. and Gregori, Marco and Vodnik, Dominik and Ferlan, Mitja and Mrak, Tanja and Straus, Ines and McDowell, Nathan G. and Kraigher, Hojka and de Marco, Ario",
year = "2017",
abstract = "Drought is an environmental stress that impacts plant productivity. Projections show both an increase in intense rain events and a reduction in the number of rain days, conditions that leads to increased risk of drought. Consequently, the identification of molecular biomarkers suitable for evaluating the impact of water deprivation conditions on forest plant seedlings is of significant value for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of drought stress coupled with variable soil temperature in European beech (Fagus sylvatica L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the specific activities and isoform profile of superoxide dismutases and glutathione peroxidases together with the variation of phenolic compounds enable discrimination between seedlings with different degrees of photosynthetic activity. This approach represents a promising platform for the assessment of drought stress in forest trees and could serve for enhancing selection and breeding practices, allowing for plants that are more tolerant of abiotic stress.",
publisher = "Canadian Science Publishing, Nrc Research Press, Ottawa",
journal = "Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere",
title = "Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica)",
volume = "47",
number = "11",
pages = "1517-1526",
doi = "10.1139/cjfr-2016-0530"
}

Popović, M. M., Gregori, M., Vodnik, D., Ferlan, M., Mrak, T., Straus, I., McDowell, N. G., Kraigher, H.,& de Marco, A.. (2017). Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica). in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere
Canadian Science Publishing, Nrc Research Press, Ottawa., 47(11), 1517-1526.
https://doi.org/10.1139/cjfr-2016-0530

Popović MM, Gregori M, Vodnik D, Ferlan M, Mrak T, Straus I, McDowell NG, Kraigher H, de Marco A. Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica). in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere. 2017;47(11):1517-1526.
doi:10.1139/cjfr-2016-0530 .

Popović, Milica M., Gregori, Marco, Vodnik, Dominik, Ferlan, Mitja, Mrak, Tanja, Straus, Ines, McDowell, Nathan G., Kraigher, Hojka, de Marco, Ario, "Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech (Fagus sylvatica)" in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere, 47, no. 11 (2017):1517-1526,
https://doi.org/10.1139/cjfr-2016-0530 . .

TY  - JOUR
AU  - Popović, Milica M.
AU  - Sustar, Vid
AU  - Gricar, Jozica
AU  - Straus, Ines
AU  - Torkar, Gregor
AU  - Kraigher, Hojka
AU  - de Marco, Ario
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2049
AB  - Climate development models predict alterations that will critically influence plant metabolism in southern and central Europe. Although the molecular players involved in the response to climatic stress factors have been well described in crops, little information is available for forest tree species. Consequently, the identification of molecular biomarkers suitable for evaluating the actual impact of different environmental stress conditions on forest plants would be of great importance for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of temperature stress in European beech (Fagus sylvatica L.) and Scots pine (Pinus sylvestris L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the combined analysis of the specific activities and isoform profile of peroxidases, superoxide dismutases, and glutathione peroxidases coupled with the amount variation of phenolic compounds enabled the discrimination between stressed and control seedlings. This approach represents a promising platform for the assessment of temperature stress in forest trees and could also enhance selection and breeding practices, allowing for plants more tolerant and (or) resistant to abiotic stress.
PB  - Canadian Science Publishing, Nrc Research Press, Ottawa
T2  - Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere
T1  - Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris)
VL  - 46
IS  - 1
SP  - 58
EP  - 66
DO  - 10.1139/cjfr-2015-0274
ER  - 

@article{
author = "Popović, Milica M. and Sustar, Vid and Gricar, Jozica and Straus, Ines and Torkar, Gregor and Kraigher, Hojka and de Marco, Ario",
year = "2016",
abstract = "Climate development models predict alterations that will critically influence plant metabolism in southern and central Europe. Although the molecular players involved in the response to climatic stress factors have been well described in crops, little information is available for forest tree species. Consequently, the identification of molecular biomarkers suitable for evaluating the actual impact of different environmental stress conditions on forest plants would be of great importance for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of temperature stress in European beech (Fagus sylvatica L.) and Scots pine (Pinus sylvestris L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the combined analysis of the specific activities and isoform profile of peroxidases, superoxide dismutases, and glutathione peroxidases coupled with the amount variation of phenolic compounds enabled the discrimination between stressed and control seedlings. This approach represents a promising platform for the assessment of temperature stress in forest trees and could also enhance selection and breeding practices, allowing for plants more tolerant and (or) resistant to abiotic stress.",
publisher = "Canadian Science Publishing, Nrc Research Press, Ottawa",
journal = "Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere",
title = "Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris)",
volume = "46",
number = "1",
pages = "58-66",
doi = "10.1139/cjfr-2015-0274"
}

Popović, M. M., Sustar, V., Gricar, J., Straus, I., Torkar, G., Kraigher, H.,& de Marco, A.. (2016). Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris). in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere
Canadian Science Publishing, Nrc Research Press, Ottawa., 46(1), 58-66.
https://doi.org/10.1139/cjfr-2015-0274

Popović MM, Sustar V, Gricar J, Straus I, Torkar G, Kraigher H, de Marco A. Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris). in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere. 2016;46(1):58-66.
doi:10.1139/cjfr-2015-0274 .

Popović, Milica M., Sustar, Vid, Gricar, Jozica, Straus, Ines, Torkar, Gregor, Kraigher, Hojka, de Marco, Ario, "Identification of environmental stress biomarkers in seedlings of European beech (Fagus sylvatica) and Scots pine (Pinus sylvestris)" in Canadian Journal of Forest Research = Revue Canadienne de La Recherche Forestiere, 46, no. 1 (2016):58-66,
https://doi.org/10.1139/cjfr-2015-0274 . .