TY  - JOUR
AU  - Cavic, Milena
AU  - Nešić, Andrijana N.
AU  - Mirjacic Martinovic, Katarina
AU  - Vuletic, Ana
AU  - Besu Zizak, Irina
AU  - Tisma Miletic, Nevena
AU  - Krivokuca, Ana
AU  - Jankovic, Radmila
AU  - Gavrović-Jankulović, Marija
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6247
AB  - This study explored humoral and cellular responses to anti-SARS-CoV-2 BNT162b2 mRNA vaccine in breastfeeding women and naïve and seropositive individuals in the first six months after vaccination.Sixty-one volunteers vaccinated with two doses of the BNT162b2 mRNA vaccine were enrolled in the study. In-house developed ELISA was used for the quantification of SARS-CoV-2 RBD-specific antibodies. Cell surface marker expression and intracellular IFN-γ analysis were carried out by flow cytometry. The concentrations of IFN-γ, IL-6 and TNF were determined by ELISA. A significant rise in anti-RBD IgG antibody levels was observed 14 days after the first vaccine dose (p < 0.0001) in serum and milk. The expression of CD28 on CD4+ T cells was significantly higher compared to baseline (p < 0.05). There was a significant increase (p ≤ 0.05) in B cell lymphocyte subset after revaccination, and increased percentage of CD80+ B cells. The expression of IFN-γ in peripheral blood lymphocytes, CD3+ T cells and serum was significantly increased (p < 0.05). No significant difference in immune response was observed between breastfeeding women and other study participants. The anti-SARS-CoV-2 BNT162b2 mRNA vaccine-induced measurable and durable immune response in breastfeeding women and in naïve and previously infected individuals.
PB  - Nature Research
T2  - Scientific Reports
T1  - Detection of humoral and cellular immune response to anti-SARS-CoV-2 BNT162b2 vaccine in breastfeeding women and naïve and previously infected individuals
VL  - 13
IS  - 1
SP  - 6271
DO  - 10.1038/s41598-023-33516-1
ER  - 

@article{
author = "Cavic, Milena and Nešić, Andrijana N. and Mirjacic Martinovic, Katarina and Vuletic, Ana and Besu Zizak, Irina and Tisma Miletic, Nevena and Krivokuca, Ana and Jankovic, Radmila and Gavrović-Jankulović, Marija",
year = "2023",
abstract = "This study explored humoral and cellular responses to anti-SARS-CoV-2 BNT162b2 mRNA vaccine in breastfeeding women and naïve and seropositive individuals in the first six months after vaccination.Sixty-one volunteers vaccinated with two doses of the BNT162b2 mRNA vaccine were enrolled in the study. In-house developed ELISA was used for the quantification of SARS-CoV-2 RBD-specific antibodies. Cell surface marker expression and intracellular IFN-γ analysis were carried out by flow cytometry. The concentrations of IFN-γ, IL-6 and TNF were determined by ELISA. A significant rise in anti-RBD IgG antibody levels was observed 14 days after the first vaccine dose (p < 0.0001) in serum and milk. The expression of CD28 on CD4+ T cells was significantly higher compared to baseline (p < 0.05). There was a significant increase (p ≤ 0.05) in B cell lymphocyte subset after revaccination, and increased percentage of CD80+ B cells. The expression of IFN-γ in peripheral blood lymphocytes, CD3+ T cells and serum was significantly increased (p < 0.05). No significant difference in immune response was observed between breastfeeding women and other study participants. The anti-SARS-CoV-2 BNT162b2 mRNA vaccine-induced measurable and durable immune response in breastfeeding women and in naïve and previously infected individuals.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Detection of humoral and cellular immune response to anti-SARS-CoV-2 BNT162b2 vaccine in breastfeeding women and naïve and previously infected individuals",
volume = "13",
number = "1",
pages = "6271",
doi = "10.1038/s41598-023-33516-1"
}

Cavic, M., Nešić, A. N., Mirjacic Martinovic, K., Vuletic, A., Besu Zizak, I., Tisma Miletic, N., Krivokuca, A., Jankovic, R.,& Gavrović-Jankulović, M.. (2023). Detection of humoral and cellular immune response to anti-SARS-CoV-2 BNT162b2 vaccine in breastfeeding women and naïve and previously infected individuals. in Scientific Reports
Nature Research., 13(1), 6271.
https://doi.org/10.1038/s41598-023-33516-1

Cavic M, Nešić AN, Mirjacic Martinovic K, Vuletic A, Besu Zizak I, Tisma Miletic N, Krivokuca A, Jankovic R, Gavrović-Jankulović M. Detection of humoral and cellular immune response to anti-SARS-CoV-2 BNT162b2 vaccine in breastfeeding women and naïve and previously infected individuals. in Scientific Reports. 2023;13(1):6271.
doi:10.1038/s41598-023-33516-1 .

Cavic, Milena, Nešić, Andrijana N., Mirjacic Martinovic, Katarina, Vuletic, Ana, Besu Zizak, Irina, Tisma Miletic, Nevena, Krivokuca, Ana, Jankovic, Radmila, Gavrović-Jankulović, Marija, "Detection of humoral and cellular immune response to anti-SARS-CoV-2 BNT162b2 vaccine in breastfeeding women and naïve and previously infected individuals" in Scientific Reports, 13, no. 1 (2023):6271,
https://doi.org/10.1038/s41598-023-33516-1 . .

TY  - JOUR
AU  - Zec, Manja
AU  - Srdić-Rajić, Tatjana
AU  - Krivokuca, Ana
AU  - Jankovic, Radmila
AU  - Todorović, Tamara
AU  - Anđelković, Katarina K.
AU  - Radulović, Siniša
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1877
AB  - The synthesis and chemical characterization of the novel 2,6-diacetylpyridine-bis(selenosemicarbazone) metal complexes of Zn(II), Cd(II) and Ni(II) were published previously. Here we report first evidence on anti-proliferative activity of the complexes and molecular patterns that underlie it. The complexes and the corresponding ligand are shown to be cytotoxic on the panel of nine, malignant and non-malignant cell lines, with the exception of Ni(II) complex that did not achieve IC50 value on any of the cell lines tested. Further experiments on the selected cell lines including A 549, MRC-5, EA.hy 926 and HeLa, have shown that the complexes posses unambiguous property of inducing necrosis in the cells treated for 6 hours, with the ligand and Zn(II) complex being the most active on all cell lines. On the contrary, only small portion of early apoptotic events was detected, under the same experimental condition. This was in complete concordance with the results obtained from Western blot analysis of the treated cells that showed no or slight increase of the protein amounts of two crucial apoptotic mediators: Cytochrome C and Caspase III. We propose the model, under which tested complexes induce necroptosis in treated cells, a recently described type of cell death with necrotic morphological features and acting via caspase independent pathway, and without elevated amounts of intracellular ROS. Endothelial EA.hy 926 cells have proven to be extremely sensitive on the necrosis-inducing effect of the complexes, which could indicate potential anti-angiogenic effect of the novel complexes that is to be investigated.
PB  - Bentham Science Publ Ltd, Sharjah
T2  - Medicinal Chemistry
T1  - Novel Selenosemicarbazone Metal Complexes Exert Anti-tumor Effect via Alternative, Caspase-independent Necroptotic Cell Death
VL  - 10
IS  - 8
SP  - 759
EP  - 771
DO  - 10.2174/1573406410666140327122009
ER  - 

@article{
author = "Zec, Manja and Srdić-Rajić, Tatjana and Krivokuca, Ana and Jankovic, Radmila and Todorović, Tamara and Anđelković, Katarina K. and Radulović, Siniša",
year = "2014",
abstract = "The synthesis and chemical characterization of the novel 2,6-diacetylpyridine-bis(selenosemicarbazone) metal complexes of Zn(II), Cd(II) and Ni(II) were published previously. Here we report first evidence on anti-proliferative activity of the complexes and molecular patterns that underlie it. The complexes and the corresponding ligand are shown to be cytotoxic on the panel of nine, malignant and non-malignant cell lines, with the exception of Ni(II) complex that did not achieve IC50 value on any of the cell lines tested. Further experiments on the selected cell lines including A 549, MRC-5, EA.hy 926 and HeLa, have shown that the complexes posses unambiguous property of inducing necrosis in the cells treated for 6 hours, with the ligand and Zn(II) complex being the most active on all cell lines. On the contrary, only small portion of early apoptotic events was detected, under the same experimental condition. This was in complete concordance with the results obtained from Western blot analysis of the treated cells that showed no or slight increase of the protein amounts of two crucial apoptotic mediators: Cytochrome C and Caspase III. We propose the model, under which tested complexes induce necroptosis in treated cells, a recently described type of cell death with necrotic morphological features and acting via caspase independent pathway, and without elevated amounts of intracellular ROS. Endothelial EA.hy 926 cells have proven to be extremely sensitive on the necrosis-inducing effect of the complexes, which could indicate potential anti-angiogenic effect of the novel complexes that is to be investigated.",
publisher = "Bentham Science Publ Ltd, Sharjah",
journal = "Medicinal Chemistry",
title = "Novel Selenosemicarbazone Metal Complexes Exert Anti-tumor Effect via Alternative, Caspase-independent Necroptotic Cell Death",
volume = "10",
number = "8",
pages = "759-771",
doi = "10.2174/1573406410666140327122009"
}

Zec, M., Srdić-Rajić, T., Krivokuca, A., Jankovic, R., Todorović, T., Anđelković, K. K.,& Radulović, S.. (2014). Novel Selenosemicarbazone Metal Complexes Exert Anti-tumor Effect via Alternative, Caspase-independent Necroptotic Cell Death. in Medicinal Chemistry
Bentham Science Publ Ltd, Sharjah., 10(8), 759-771.
https://doi.org/10.2174/1573406410666140327122009

Zec M, Srdić-Rajić T, Krivokuca A, Jankovic R, Todorović T, Anđelković KK, Radulović S. Novel Selenosemicarbazone Metal Complexes Exert Anti-tumor Effect via Alternative, Caspase-independent Necroptotic Cell Death. in Medicinal Chemistry. 2014;10(8):759-771.
doi:10.2174/1573406410666140327122009 .

Zec, Manja, Srdić-Rajić, Tatjana, Krivokuca, Ana, Jankovic, Radmila, Todorović, Tamara, Anđelković, Katarina K., Radulović, Siniša, "Novel Selenosemicarbazone Metal Complexes Exert Anti-tumor Effect via Alternative, Caspase-independent Necroptotic Cell Death" in Medicinal Chemistry, 10, no. 8 (2014):759-771,
https://doi.org/10.2174/1573406410666140327122009 . .

TY  - JOUR
AU  - Čavić, Milena
AU  - Grozdanović, Milica M.
AU  - Bajić, Aleksandar
AU  - Jankovic, Radmila
AU  - Andjus, Pavle R.
AU  - Gavrović-Jankulović, Marija
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1866
AB  - Actinidin, a kiwifruit cysteine protease, is a marker allergen for genuine sensitization to this food allergen source. Inhalatory cysteine proteases have the capacity for disruption of tight junctions (TJs) enhancing the permeability of the bronchial epithelium. No such properties have been reported for allergenic food proteases so far. The aim was to determine the effect of actinidin on the integrity of T84 monolayers by evaluating its action on the TJ protein occludin. Immunoblot and immunofluorescence were employed for the detection of occludin protein alterations. Gene expression was evaluated by RT-PCR. Breach of occludin network was assessed by measuring transepithelial resistance, blue dextran leakage and passage of allergens from the apical to basolateral compartment. Actinidin exerted direct proteolytic cleavage of occludin; no alteration of occludin gene expression was detected. There was a reduction of occludin staining upon actinidin treatment as a consequence of its degradation and dispersion within the membrane. There was an increase in permeability of the T84 monolayer resulting in reduced transepithelial resistance, blue dextran leakage and passage of allergens actinidin and thaumatin-like protein from the apical to basolateral compartment. Opening of TJs by actinidin may increase intestinal permeability and contribute to the process of sensitization in kiwifruit allergy. (C) 2014 Elsevier Ltd. All rights reserved.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - The effect of kiwifruit (Actinidia deliciosa) cysteine protease actinidin on the occludin tight junction network in T84 intestinal epithelial cells
VL  - 72
SP  - 61
EP  - 68
DO  - 10.1016/j.fct.2014.07.012
ER  - 

@article{
author = "Čavić, Milena and Grozdanović, Milica M. and Bajić, Aleksandar and Jankovic, Radmila and Andjus, Pavle R. and Gavrović-Jankulović, Marija",
year = "2014",
abstract = "Actinidin, a kiwifruit cysteine protease, is a marker allergen for genuine sensitization to this food allergen source. Inhalatory cysteine proteases have the capacity for disruption of tight junctions (TJs) enhancing the permeability of the bronchial epithelium. No such properties have been reported for allergenic food proteases so far. The aim was to determine the effect of actinidin on the integrity of T84 monolayers by evaluating its action on the TJ protein occludin. Immunoblot and immunofluorescence were employed for the detection of occludin protein alterations. Gene expression was evaluated by RT-PCR. Breach of occludin network was assessed by measuring transepithelial resistance, blue dextran leakage and passage of allergens from the apical to basolateral compartment. Actinidin exerted direct proteolytic cleavage of occludin; no alteration of occludin gene expression was detected. There was a reduction of occludin staining upon actinidin treatment as a consequence of its degradation and dispersion within the membrane. There was an increase in permeability of the T84 monolayer resulting in reduced transepithelial resistance, blue dextran leakage and passage of allergens actinidin and thaumatin-like protein from the apical to basolateral compartment. Opening of TJs by actinidin may increase intestinal permeability and contribute to the process of sensitization in kiwifruit allergy. (C) 2014 Elsevier Ltd. All rights reserved.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "The effect of kiwifruit (Actinidia deliciosa) cysteine protease actinidin on the occludin tight junction network in T84 intestinal epithelial cells",
volume = "72",
pages = "61-68",
doi = "10.1016/j.fct.2014.07.012"
}

Čavić, M., Grozdanović, M. M., Bajić, A., Jankovic, R., Andjus, P. R.,& Gavrović-Jankulović, M.. (2014). The effect of kiwifruit (Actinidia deliciosa) cysteine protease actinidin on the occludin tight junction network in T84 intestinal epithelial cells. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 72, 61-68.
https://doi.org/10.1016/j.fct.2014.07.012

Čavić M, Grozdanović MM, Bajić A, Jankovic R, Andjus PR, Gavrović-Jankulović M. The effect of kiwifruit (Actinidia deliciosa) cysteine protease actinidin on the occludin tight junction network in T84 intestinal epithelial cells. in Food and Chemical Toxicology. 2014;72:61-68.
doi:10.1016/j.fct.2014.07.012 .

Čavić, Milena, Grozdanović, Milica M., Bajić, Aleksandar, Jankovic, Radmila, Andjus, Pavle R., Gavrović-Jankulović, Marija, "The effect of kiwifruit (Actinidia deliciosa) cysteine protease actinidin on the occludin tight junction network in T84 intestinal epithelial cells" in Food and Chemical Toxicology, 72 (2014):61-68,
https://doi.org/10.1016/j.fct.2014.07.012 . .

TY  - JOUR
AU  - Filipović, Lana
AU  - Aranđelović, Sandra
AU  - Gligorijević, Nevenka
AU  - Krivokuca, Ana
AU  - Jankovic, Radmila
AU  - Srdić-Rajić, Tatjana
AU  - Rakic, Gordana
AU  - Tešić, Živoslav Lj.
AU  - Radulović, Siniša
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1414
AB  - Background. In our previous study we reported the synthesis and cytotoxicity of two trans-platinum(II) complexes: trans-[PtCl2(3-acetylpyridine)(2)] (1) and trans-[PtCl2(4-acetylpyridine)(2)] (2), revealing significant cytotoxic potential of 2. In order to evaluate the mechanism underlying biological activity of both trans-Pt(II) isomers, comparative studies versus cisplatin were performed in HeLa, MRC-5 and MS1 cells. Materials and methods. The cytotoxic activity of the investigated complexes was determined using SRB assay. The colagenolytic activity was determined using gelatin zymography, while the effect of platinum complexes on matrix metalloproteinases 2 and 9 mRNA expression was evaluated by quantitative real-time PCR. Apoptotic potential and cell cycle alterations were determined by FACS analyses. Western blot analysis was used to evaluate the effect on expression of DNA-repair enzyme ERCC1, and quantitative real-time PCR was used for the ERCC1 mRNA expression analysis. In vitro antiangiogenic potential was determined by tube formation assay. Platinum content in intracellular DNA and proteins was determined by inductively coupled plasma-optical emission spectrometry. Results. Compound 2 displayed an apparent cytoselective profile, and flow cytometry analysis in HeLa cells indicated that 2 exerted antiproliferative effect through apoptosis induction, while 1 induced both apoptosis and necrosis. Action of 1 and 2, as analyzed by quantitative real-time PCR and Western blot, was associated with down-regulation of ERCC1. Both trans-complexes inhibited MMP-9 mRNA expression in HeLa, while 2 significantly abrogated in vitro tubulogenesis in MS1 cells. Conclusions. The ability of 2 to induce multiple and selective in vitro cytotoxic effects encourages further investigations of trans-platinum(II) complexes with substituted pyridines.
PB  - Assoc Radiology & Oncology, Ljubljana
T2  - Radiology and Oncology
T1  - Biological evaluation of transdichloridoplatinum( II) complexes with 3-and 4-acetylpyridine in comparison to cisplatin
VL  - 47
IS  - 4
SP  - 346
EP  - 357
DO  - 10.2478/raon-2013-0050
ER  - 

@article{
author = "Filipović, Lana and Aranđelović, Sandra and Gligorijević, Nevenka and Krivokuca, Ana and Jankovic, Radmila and Srdić-Rajić, Tatjana and Rakic, Gordana and Tešić, Živoslav Lj. and Radulović, Siniša",
year = "2013",
abstract = "Background. In our previous study we reported the synthesis and cytotoxicity of two trans-platinum(II) complexes: trans-[PtCl2(3-acetylpyridine)(2)] (1) and trans-[PtCl2(4-acetylpyridine)(2)] (2), revealing significant cytotoxic potential of 2. In order to evaluate the mechanism underlying biological activity of both trans-Pt(II) isomers, comparative studies versus cisplatin were performed in HeLa, MRC-5 and MS1 cells. Materials and methods. The cytotoxic activity of the investigated complexes was determined using SRB assay. The colagenolytic activity was determined using gelatin zymography, while the effect of platinum complexes on matrix metalloproteinases 2 and 9 mRNA expression was evaluated by quantitative real-time PCR. Apoptotic potential and cell cycle alterations were determined by FACS analyses. Western blot analysis was used to evaluate the effect on expression of DNA-repair enzyme ERCC1, and quantitative real-time PCR was used for the ERCC1 mRNA expression analysis. In vitro antiangiogenic potential was determined by tube formation assay. Platinum content in intracellular DNA and proteins was determined by inductively coupled plasma-optical emission spectrometry. Results. Compound 2 displayed an apparent cytoselective profile, and flow cytometry analysis in HeLa cells indicated that 2 exerted antiproliferative effect through apoptosis induction, while 1 induced both apoptosis and necrosis. Action of 1 and 2, as analyzed by quantitative real-time PCR and Western blot, was associated with down-regulation of ERCC1. Both trans-complexes inhibited MMP-9 mRNA expression in HeLa, while 2 significantly abrogated in vitro tubulogenesis in MS1 cells. Conclusions. The ability of 2 to induce multiple and selective in vitro cytotoxic effects encourages further investigations of trans-platinum(II) complexes with substituted pyridines.",
publisher = "Assoc Radiology & Oncology, Ljubljana",
journal = "Radiology and Oncology",
title = "Biological evaluation of transdichloridoplatinum( II) complexes with 3-and 4-acetylpyridine in comparison to cisplatin",
volume = "47",
number = "4",
pages = "346-357",
doi = "10.2478/raon-2013-0050"
}

Filipović, L., Aranđelović, S., Gligorijević, N., Krivokuca, A., Jankovic, R., Srdić-Rajić, T., Rakic, G., Tešić, Ž. Lj.,& Radulović, S.. (2013). Biological evaluation of transdichloridoplatinum( II) complexes with 3-and 4-acetylpyridine in comparison to cisplatin. in Radiology and Oncology
Assoc Radiology & Oncology, Ljubljana., 47(4), 346-357.
https://doi.org/10.2478/raon-2013-0050

Filipović L, Aranđelović S, Gligorijević N, Krivokuca A, Jankovic R, Srdić-Rajić T, Rakic G, Tešić ŽL, Radulović S. Biological evaluation of transdichloridoplatinum( II) complexes with 3-and 4-acetylpyridine in comparison to cisplatin. in Radiology and Oncology. 2013;47(4):346-357.
doi:10.2478/raon-2013-0050 .

Filipović, Lana, Aranđelović, Sandra, Gligorijević, Nevenka, Krivokuca, Ana, Jankovic, Radmila, Srdić-Rajić, Tatjana, Rakic, Gordana, Tešić, Živoslav Lj., Radulović, Siniša, "Biological evaluation of transdichloridoplatinum( II) complexes with 3-and 4-acetylpyridine in comparison to cisplatin" in Radiology and Oncology, 47, no. 4 (2013):346-357,
https://doi.org/10.2478/raon-2013-0050 . .

TY  - JOUR
AU  - Gligorijević, Nevenka
AU  - Aranđelović, Sandra
AU  - Filipović, Lana
AU  - Jakovljević, Ksenija
AU  - Jankovic, Radmila
AU  - Grgurić-Šipka, Sanja
AU  - Ivanović, Ivanka
AU  - Radulović, Siniša
AU  - Tešić, Živoslav Lj.
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1270
AB  - In our previous study, ruthenium(II)-p-cymene complexes of general formula [(eta(6)-p-cymene)Ru(L)Cl2], L: 3-acetylpyridine (1), 2-amino-5-chloropyridine (2); and [(eta(6)-p-cymene)Ru(HL)Cl], HL: 2,3-pyridinedicarboxylic acid (3), 2,4-pyridinedicarboxylic acid (4), revealed low antiproliferative activity, except complex [(eta(6)-p-cymene)RuCl(picolinic acid)]center dot H2O (5) which exhibited IC50 around 80 mu M. In this study we further investigated in vitro potential of antimetastatic action of ruthenium complexes on HeLa and two endothelial cell lines. Comparison of structure and activity of five complexes indicated heterogenic mode of activity, with regard to the potential of antimetastatic and antiproliferative effect. Replacement of substituted pyridine ligand with picolinic acid (complex 5) around Ru(II) center contributed to complex cytotoxicity and ruthenium DNA binding affinity. Analysis of ruthenium(II) accumulation in DNA and protein fractions of HeLa cells, using ICP-OES revealed significantly higher content of complex 5 in DNA fraction in comparison to the other tested compounds. It also altered cell cycle progression, affected expression of DNA repair enzymes ERCC1 and MSH2, and showed enhanced activity in combination with 3-aminobenzamide. Regardless of their effect on cell growth, Ru(II) complexes exerted antimetastatic effect on several tumor cell lines in vitro, achieved mostly by the effect on cell adhesion, migration and angiogenesis, while picolinate ruthenium(II)-arene additionally exerted inhibitory effect on extracellular matrix degradation. (c) 2011 Elsevier Inc. All rights reserved.
PB  - Elsevier Science Inc, New York
T2  - Journal of Inorganic Biochemistry
T1  - Picolinate ruthenium(II)-arene complex with in vitro antiproliferative and antimetastatic properties: Comparison to a series of ruthenium(II)-arene complexes with similar structure
VL  - 108
SP  - 53
EP  - 61
DO  - 10.1016/j.jinorgbio.2011.12.002
ER  - 

@article{
author = "Gligorijević, Nevenka and Aranđelović, Sandra and Filipović, Lana and Jakovljević, Ksenija and Jankovic, Radmila and Grgurić-Šipka, Sanja and Ivanović, Ivanka and Radulović, Siniša and Tešić, Živoslav Lj.",
year = "2012",
abstract = "In our previous study, ruthenium(II)-p-cymene complexes of general formula [(eta(6)-p-cymene)Ru(L)Cl2], L: 3-acetylpyridine (1), 2-amino-5-chloropyridine (2); and [(eta(6)-p-cymene)Ru(HL)Cl], HL: 2,3-pyridinedicarboxylic acid (3), 2,4-pyridinedicarboxylic acid (4), revealed low antiproliferative activity, except complex [(eta(6)-p-cymene)RuCl(picolinic acid)]center dot H2O (5) which exhibited IC50 around 80 mu M. In this study we further investigated in vitro potential of antimetastatic action of ruthenium complexes on HeLa and two endothelial cell lines. Comparison of structure and activity of five complexes indicated heterogenic mode of activity, with regard to the potential of antimetastatic and antiproliferative effect. Replacement of substituted pyridine ligand with picolinic acid (complex 5) around Ru(II) center contributed to complex cytotoxicity and ruthenium DNA binding affinity. Analysis of ruthenium(II) accumulation in DNA and protein fractions of HeLa cells, using ICP-OES revealed significantly higher content of complex 5 in DNA fraction in comparison to the other tested compounds. It also altered cell cycle progression, affected expression of DNA repair enzymes ERCC1 and MSH2, and showed enhanced activity in combination with 3-aminobenzamide. Regardless of their effect on cell growth, Ru(II) complexes exerted antimetastatic effect on several tumor cell lines in vitro, achieved mostly by the effect on cell adhesion, migration and angiogenesis, while picolinate ruthenium(II)-arene additionally exerted inhibitory effect on extracellular matrix degradation. (c) 2011 Elsevier Inc. All rights reserved.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Inorganic Biochemistry",
title = "Picolinate ruthenium(II)-arene complex with in vitro antiproliferative and antimetastatic properties: Comparison to a series of ruthenium(II)-arene complexes with similar structure",
volume = "108",
pages = "53-61",
doi = "10.1016/j.jinorgbio.2011.12.002"
}

Gligorijević, N., Aranđelović, S., Filipović, L., Jakovljević, K., Jankovic, R., Grgurić-Šipka, S., Ivanović, I., Radulović, S.,& Tešić, Ž. Lj.. (2012). Picolinate ruthenium(II)-arene complex with in vitro antiproliferative and antimetastatic properties: Comparison to a series of ruthenium(II)-arene complexes with similar structure. in Journal of Inorganic Biochemistry
Elsevier Science Inc, New York., 108, 53-61.
https://doi.org/10.1016/j.jinorgbio.2011.12.002

Gligorijević N, Aranđelović S, Filipović L, Jakovljević K, Jankovic R, Grgurić-Šipka S, Ivanović I, Radulović S, Tešić ŽL. Picolinate ruthenium(II)-arene complex with in vitro antiproliferative and antimetastatic properties: Comparison to a series of ruthenium(II)-arene complexes with similar structure. in Journal of Inorganic Biochemistry. 2012;108:53-61.
doi:10.1016/j.jinorgbio.2011.12.002 .

Gligorijević, Nevenka, Aranđelović, Sandra, Filipović, Lana, Jakovljević, Ksenija, Jankovic, Radmila, Grgurić-Šipka, Sanja, Ivanović, Ivanka, Radulović, Siniša, Tešić, Živoslav Lj., "Picolinate ruthenium(II)-arene complex with in vitro antiproliferative and antimetastatic properties: Comparison to a series of ruthenium(II)-arene complexes with similar structure" in Journal of Inorganic Biochemistry, 108 (2012):53-61,
https://doi.org/10.1016/j.jinorgbio.2011.12.002 . .