Gavrović-Jankulović, Marija

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Authority KeyName Variants
orcid::0000-0002-8591-4391
  • Gavrović-Jankulović, Marija (145)
Projects
Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance Ispitivanje strukture i funkcije biološki važnih makromolekula u fiziološkim i patološkim stanjima
Reinforcement of the Faculty of Chemistry, University of Belgrade, towards becoming a Center of Excellence in the region of WB for Molecular Biotechnology and Food research Application of advanced oxidation processes and nanostructured oxide materials for the removal of pollutants from the environment, development and optimisation of instrumental techniques for efficiency monitoring
Molecular properties and modifications of some respiratory and nutritional allergens Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness
Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering Bioanalytical Chemistry Research Group at the Research Center Borstel
COST Action FA1402 entitled: Improving Allergy Risk Assessment Strategy for New Food Proteins (ImpARAS) COST action [FA1402 ImpARAS]
German Academic Exchange Service DAAD Research [91530210-50015537] High-Performance Computing Infrastructure for South East Europe's Research Communities
Rational design and synthesis of biologically active and coordination compounds and functional materials, relevant for (bio)nanotechnology Production, purification and characterization of enzymes and small molecules and their application as soluble or immobilized in food biotechnology, biofuels production and environmental protection
Interactions of natural products, their derivatives and coordination compounds with proteins and nucleic acids Ministry of Sciences of Croatia
University of Rijeka [13.11.1.2.03] FEBS Collaborative and Experimental Scholarship for Central and Eastern Europe
Beneficial effects of dietary bioactive peptides and polyphenols on cardiovascular health in humans Clinical-epidemiological research of diseases of public health importance in Serbia
The pathogenetic mechanism in hematological malignancies Biological effects, nutritional intake and status of folate and polysaturate fatty acid (PUFA): improvement of nutrition in Serbia
Agrobiodiversity and land-use change in Serbia: an integrated biodiversity assessment of key functional groups of arthropods and plant pathogens Development of new information and communication technologies, based on advanced mathematical methods, with applications in medicine, telecommunications, power systems, protection of national heritage and education
Regulacija antioksidativnog metabolizma biljaka u toku rastenja, infekcije patogenima i delovanja abiotičkog stresa: mehanizmi transporta, signalizacije i otpornosti Institute for Risk Assessment Sciences, University of Utrecht,Netherlands, which was supported by COST Action FA1402.
Magbiovin project (FP7- ERAChairs-Pilot Call-2013, Grant agreement: 621375)

Author's Bibliography

Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease

Stojsavljević, Aleksandar; Rovčanin, Branislav; Krstić, Đurđa D.; Borković-Mitić, Slavica S.; Paunović, Ivan; Diklić, Aleksandar; Gavrović-Jankulović, Marija; Manojlović, Dragan D.

(2020)

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa D.
AU  - Borković-Mitić, Slavica S.
AU  - Paunović, Ivan
AU  - Diklić, Aleksandar
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan D.
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3955
AB  - The background level of lead (Pb) in drinking water in Serbia represents a significant health issue. The aim of study was to examine the content of significant toxic and essential trace metals (Mn, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U) in the thyroid tissues with diagnosed colloid goiter (CG). Different variables were investigated (sex, age, smoking habits, goiter size, and previous history of any thyroid disease). The study was designed to compare the goiter tissues (GTs) to sex- and age-matched healthy- and malignant-thyroid tissues (HTTs and MTTs, respectively). All trace metals were quantified by inductively coupled plasma mass spectrometry (ICP-MS). The reduced content of essential elements (Mn and Se) and increased content of toxic metals (Pb, Th, and U) were found in GTs compared with HTTs. The increased content of the same toxic metals separated GTs from MTTs, as well as reduced content of Mn and Cd. Our results indicated that Pb acts as the main goitrogen, which could highlight its role in the unknown etiology of CG disease. Pb/Se ratio could be considered as a relevant parameter for the tissue separation of GTs from HTTs and/or MTTs. This research also provides novel data on the effects of metal pollutants on thyroid health hazards. Graphical Abstract: [Figure not available: see fulltext.].
T2  - Exposure and Health
T1  - Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease
VL  - 12
IS  - 2
SP  - 255
EP  - 264
DO  - 10.1007/s12403-019-00309-9
ER  - 
@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa D. and Borković-Mitić, Slavica S. and Paunović, Ivan and Diklić, Aleksandar and Gavrović-Jankulović, Marija and Manojlović, Dragan D.",
year = "2020",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3955",
abstract = "The background level of lead (Pb) in drinking water in Serbia represents a significant health issue. The aim of study was to examine the content of significant toxic and essential trace metals (Mn, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U) in the thyroid tissues with diagnosed colloid goiter (CG). Different variables were investigated (sex, age, smoking habits, goiter size, and previous history of any thyroid disease). The study was designed to compare the goiter tissues (GTs) to sex- and age-matched healthy- and malignant-thyroid tissues (HTTs and MTTs, respectively). All trace metals were quantified by inductively coupled plasma mass spectrometry (ICP-MS). The reduced content of essential elements (Mn and Se) and increased content of toxic metals (Pb, Th, and U) were found in GTs compared with HTTs. The increased content of the same toxic metals separated GTs from MTTs, as well as reduced content of Mn and Cd. Our results indicated that Pb acts as the main goitrogen, which could highlight its role in the unknown etiology of CG disease. Pb/Se ratio could be considered as a relevant parameter for the tissue separation of GTs from HTTs and/or MTTs. This research also provides novel data on the effects of metal pollutants on thyroid health hazards. Graphical Abstract: [Figure not available: see fulltext.].",
journal = "Exposure and Health",
title = "Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease",
volume = "12",
number = "2",
pages = "255-264",
doi = "10.1007/s12403-019-00309-9"
}
Stojsavljević, A., Rovčanin, B., Krstić, Đ. D., Borković-Mitić, S. S., Paunović, I., Diklić, A., Gavrović-Jankulović, M.,& Manojlović, D. D. (2020). Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease.
Exposure and Health, 12(2), 255-264.
https://doi.org/10.1007/s12403-019-00309-9
Stojsavljević A, Rovčanin B, Krstić ĐD, Borković-Mitić SS, Paunović I, Diklić A, Gavrović-Jankulović M, Manojlović DD. Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease. Exposure and Health. 2020;12(2):255-264
Stojsavljević Aleksandar, Rovčanin Branislav, Krstić Đurđa D., Borković-Mitić Slavica S., Paunović Ivan, Diklić Aleksandar, Gavrović-Jankulović Marija, Manojlović Dragan D., "Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease" Exposure and Health, 12, no. 2 (2020):255-264,
https://doi.org/10.1007/s12403-019-00309-9 .
5
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Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors

Stojsavljević, Aleksandar; Vujotić, Ljiljana; Rovčanin, Branislav; Borković-Mitić, Slavica S.; Gavrović-Jankulović, Marija; Manojlović, Dragan D.

(Nature Research, 2020)

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Vujotić, Ljiljana
AU  - Rovčanin, Branislav
AU  - Borković-Mitić, Slavica S.
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan D.
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3885
AB  - The pathogenesis of malignant brain tumors (MBTs) should be better understood due to the evident association between prolonged exposure to metals and increased risk of MBTs. The present research aimed to find trace metals that could contribute to the pathogenesis of MBTs. Essential trace elements (Mn, Co, Zn, Cu, Se) and relevant toxic metals (Al, Ni, As, Sr, Cd, Ce, Pt, Pb, U) in the serum, cell fraction (CF), cerebrospinal fluid (CSF) and cancerous tissue (CT) samples of MBT patients were analyzed. The results were compared with sex- and age-matched control groups. For the first time, this research showed that elemental profiles of serum, CF, CSF and CT samples in MBT patients were significantly altered compared to the appropriate controls, as well as that higher contents of trace elements (particularly Mn, Se, and Pb) could be involved in the pathogenesis of MBTs. However, the most noticeable change found was the elevated U content, indicating its considerable role as a major cerebral discriminator of the presence/absence of MBTs. The U/Se ratio could be considered as an appropriate blood marker in diagnostic MBT evaluation. The reported results could contribute to better understanding of the poorly understood pathogenesis of MBTs. Furthermore, the reported results could highlight a molecular basis for the pathophysiological changes caused by the hazardous effects of trace metals on brain homeostasis.
PB  - Nature Research
T2  - Scientific Reports
T1  - Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors
VL  - 10
IS  - 1
DO  - 10.1038/s41598-020-60774-0
ER  - 
@article{
author = "Stojsavljević, Aleksandar and Vujotić, Ljiljana and Rovčanin, Branislav and Borković-Mitić, Slavica S. and Gavrović-Jankulović, Marija and Manojlović, Dragan D.",
year = "2020",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3885",
abstract = "The pathogenesis of malignant brain tumors (MBTs) should be better understood due to the evident association between prolonged exposure to metals and increased risk of MBTs. The present research aimed to find trace metals that could contribute to the pathogenesis of MBTs. Essential trace elements (Mn, Co, Zn, Cu, Se) and relevant toxic metals (Al, Ni, As, Sr, Cd, Ce, Pt, Pb, U) in the serum, cell fraction (CF), cerebrospinal fluid (CSF) and cancerous tissue (CT) samples of MBT patients were analyzed. The results were compared with sex- and age-matched control groups. For the first time, this research showed that elemental profiles of serum, CF, CSF and CT samples in MBT patients were significantly altered compared to the appropriate controls, as well as that higher contents of trace elements (particularly Mn, Se, and Pb) could be involved in the pathogenesis of MBTs. However, the most noticeable change found was the elevated U content, indicating its considerable role as a major cerebral discriminator of the presence/absence of MBTs. The U/Se ratio could be considered as an appropriate blood marker in diagnostic MBT evaluation. The reported results could contribute to better understanding of the poorly understood pathogenesis of MBTs. Furthermore, the reported results could highlight a molecular basis for the pathophysiological changes caused by the hazardous effects of trace metals on brain homeostasis.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors",
volume = "10",
number = "1",
doi = "10.1038/s41598-020-60774-0"
}
Stojsavljević, A., Vujotić, L., Rovčanin, B., Borković-Mitić, S. S., Gavrović-Jankulović, M.,& Manojlović, D. D. (2020). Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors.
Scientific Reports
Nature Research., 10(1).
https://doi.org/10.1038/s41598-020-60774-0
Stojsavljević A, Vujotić L, Rovčanin B, Borković-Mitić SS, Gavrović-Jankulović M, Manojlović DD. Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors. Scientific Reports. 2020;10(1)
Stojsavljević Aleksandar, Vujotić Ljiljana, Rovčanin Branislav, Borković-Mitić Slavica S., Gavrović-Jankulović Marija, Manojlović Dragan D., "Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors" Scientific Reports, 10, no. 1 (2020),
https://doi.org/10.1038/s41598-020-60774-0 .
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Significance of arsenic and lead in Hashimoto's thyroiditis demonstrated on thyroid tissue, blood, and urine samples

Stojsavljević, Aleksandar; Rovčanin, Branislav; Jagodić, Jovana; Drašković Radojković, Danijela; Paunović, Ivan; Gavrović-Jankulović, Marija; Manojlović, Dragan D.

(Elsevier, 2020)

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Jagodić, Jovana
AU  - Drašković Radojković, Danijela
AU  - Paunović, Ivan
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan D.
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4032
AB  - Our previous investigation showed significantly increased arsenic (As) content in thyroid tissue samples of patients with Hashimoto's thyroiditis (HT). This research aimed to extend previous findings and provide reliable insight into the close relationship between As and other trace elements with HT by considering a greater number of thyroid tissue samples, accompanied by blood and urine samples. The essential trace elements for thyroid homeostasis (Mn, Cu, Zn, Se) and the main threatening toxic trace elements (Ni, As, Pb, Cd, U) was analyzed by inductively coupled plasma-mass spectrometry (ICP-MS). Relevant parameters that could affect the concentration of trace elements were considered. This research showed that there was a difference in the elemental profile between HT and control samples. The most important findings were related to the elevated As and Pb content in the thyroid tissue and HT blood samples. The obtained negative correlations between As and Pb with Se may explain the antagonistic effect of As and Pb on the extrusion of essential Se from the HT tissue. The reduced Se content in the blood and its increased content in urine samples may further confirm this hypothesis and explain the lack of Se in HT. Furthermore, the reported results may highlight the unresolved molecular basis of HT and could indicate the role of trace element effects on thyroid homeostasis.
PB  - Elsevier
T2  - Environmental Research
T1  - Significance of arsenic and lead in Hashimoto's thyroiditis demonstrated on thyroid tissue, blood, and urine samples
VL  - 186
SP  - 109538
DO  - 10.1016/j.envres.2020.109538
ER  - 
@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Jagodić, Jovana and Drašković Radojković, Danijela and Paunović, Ivan and Gavrović-Jankulović, Marija and Manojlović, Dragan D.",
year = "2020",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/4032",
abstract = "Our previous investigation showed significantly increased arsenic (As) content in thyroid tissue samples of patients with Hashimoto's thyroiditis (HT). This research aimed to extend previous findings and provide reliable insight into the close relationship between As and other trace elements with HT by considering a greater number of thyroid tissue samples, accompanied by blood and urine samples. The essential trace elements for thyroid homeostasis (Mn, Cu, Zn, Se) and the main threatening toxic trace elements (Ni, As, Pb, Cd, U) was analyzed by inductively coupled plasma-mass spectrometry (ICP-MS). Relevant parameters that could affect the concentration of trace elements were considered. This research showed that there was a difference in the elemental profile between HT and control samples. The most important findings were related to the elevated As and Pb content in the thyroid tissue and HT blood samples. The obtained negative correlations between As and Pb with Se may explain the antagonistic effect of As and Pb on the extrusion of essential Se from the HT tissue. The reduced Se content in the blood and its increased content in urine samples may further confirm this hypothesis and explain the lack of Se in HT. Furthermore, the reported results may highlight the unresolved molecular basis of HT and could indicate the role of trace element effects on thyroid homeostasis.",
publisher = "Elsevier",
journal = "Environmental Research",
title = "Significance of arsenic and lead in Hashimoto's thyroiditis demonstrated on thyroid tissue, blood, and urine samples",
volume = "186",
pages = "109538",
doi = "10.1016/j.envres.2020.109538"
}
Stojsavljević, A., Rovčanin, B., Jagodić, J., Drašković Radojković, D., Paunović, I., Gavrović-Jankulović, M.,& Manojlović, D. D. (2020). Significance of arsenic and lead in Hashimoto's thyroiditis demonstrated on thyroid tissue, blood, and urine samples.
Environmental Research
Elsevier., 186, 109538.
https://doi.org/10.1016/j.envres.2020.109538
Stojsavljević A, Rovčanin B, Jagodić J, Drašković Radojković D, Paunović I, Gavrović-Jankulović M, Manojlović DD. Significance of arsenic and lead in Hashimoto's thyroiditis demonstrated on thyroid tissue, blood, and urine samples. Environmental Research. 2020;186:109538
Stojsavljević Aleksandar, Rovčanin Branislav, Jagodić Jovana, Drašković Radojković Danijela, Paunović Ivan, Gavrović-Jankulović Marija, Manojlović Dragan D., "Significance of arsenic and lead in Hashimoto's thyroiditis demonstrated on thyroid tissue, blood, and urine samples" Environmental Research, 186 (2020):109538,
https://doi.org/10.1016/j.envres.2020.109538 .
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Impact of tree pollen distribution on allergic diseases in serbia: Evidence of implementation of allergen immunotherapy to Betula verrucosa

Minić, Rajna; Josipović, Mirjana; Tomić Spirić, Vesna; Gavrović-Jankulović, Marija; Perić Popadić, Aleksandra; Prokopijević, Ivana; Ljubičić, Ana; Stamenković, Danijela; Burazer, Lidija M.

(MDPI, 2020)

TY  - JOUR
AU  - Minić, Rajna
AU  - Josipović, Mirjana
AU  - Tomić Spirić, Vesna
AU  - Gavrović-Jankulović, Marija
AU  - Perić Popadić, Aleksandra
AU  - Prokopijević, Ivana
AU  - Ljubičić, Ana
AU  - Stamenković, Danijela
AU  - Burazer, Lidija M.
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3883
AB  - Background and objectives: The relationship between air pollen quantity and the sensitization of allergic patients is crucial for both the diagnosis and treatment of allergic diseases. Weather conditions influence the distribution of allergenic pollen and increases in pollen concentration may negatively affect the health of allergic patients. The aim of this study was to analyze the implementation of allergen immunotherapy with regard to air pollen concentration. Material and Methods: Here we examined the relationship between Betula air pollen concentration and the usage of Betula verrucosa allergen immunotherapy in Serbia. Examination covered the period from 2015 to 2018. Measurement of airborne pollen concentration was performed with Lanzoni volumetric pollen traps. The evidence of the usage of sublingual allergen immunotherapy (SLIT) was gathered from patients with documented sensitization to specific pollen. Results: During this period tree pollens were represented with 58% ± 21% of all measured air pollen species, while Betula pollen represented 15% ± 8% of all tree pollens. Betula pollination peaked in April. Allergen immunotherapy to Betula verrucosa in Serbia is entirely conducted as sublingual immunotherapy and represents 47.1% ± 1.4% of issued tree pollen SLIT. The use of pollen SLIT increased by 68% from 2015 to 2018, with an even greater increase in usage recorded for Betula SLIT—80%. Conclusions: This analysis shows a clear causative relationship between pollination and the type/prevalence of applied allergen immunotherapy. Information about the flowering seasons of allergenic plants is very important for people who suffer from allergy, for clinical allergologists, as well as for governing authorities. The presented data is of practical importance to the proper timing of immunotherapy initiation and of importance for urban landscaping. The obtained data can be the starting point for the instatement of a thorough epidemiological study and the inclusion of Serbia on the pollen map of Europe.
PB  - MDPI
T2  - Medicina (Lithuania)
T1  - Impact of tree pollen distribution on allergic diseases in serbia: Evidence of implementation of allergen immunotherapy to Betula verrucosa
VL  - 56
IS  - 2
DO  - 10.3390/medicina56020059
ER  - 
@article{
author = "Minić, Rajna and Josipović, Mirjana and Tomić Spirić, Vesna and Gavrović-Jankulović, Marija and Perić Popadić, Aleksandra and Prokopijević, Ivana and Ljubičić, Ana and Stamenković, Danijela and Burazer, Lidija M.",
year = "2020",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3883",
abstract = "Background and objectives: The relationship between air pollen quantity and the sensitization of allergic patients is crucial for both the diagnosis and treatment of allergic diseases. Weather conditions influence the distribution of allergenic pollen and increases in pollen concentration may negatively affect the health of allergic patients. The aim of this study was to analyze the implementation of allergen immunotherapy with regard to air pollen concentration. Material and Methods: Here we examined the relationship between Betula air pollen concentration and the usage of Betula verrucosa allergen immunotherapy in Serbia. Examination covered the period from 2015 to 2018. Measurement of airborne pollen concentration was performed with Lanzoni volumetric pollen traps. The evidence of the usage of sublingual allergen immunotherapy (SLIT) was gathered from patients with documented sensitization to specific pollen. Results: During this period tree pollens were represented with 58% ± 21% of all measured air pollen species, while Betula pollen represented 15% ± 8% of all tree pollens. Betula pollination peaked in April. Allergen immunotherapy to Betula verrucosa in Serbia is entirely conducted as sublingual immunotherapy and represents 47.1% ± 1.4% of issued tree pollen SLIT. The use of pollen SLIT increased by 68% from 2015 to 2018, with an even greater increase in usage recorded for Betula SLIT—80%. Conclusions: This analysis shows a clear causative relationship between pollination and the type/prevalence of applied allergen immunotherapy. Information about the flowering seasons of allergenic plants is very important for people who suffer from allergy, for clinical allergologists, as well as for governing authorities. The presented data is of practical importance to the proper timing of immunotherapy initiation and of importance for urban landscaping. The obtained data can be the starting point for the instatement of a thorough epidemiological study and the inclusion of Serbia on the pollen map of Europe.",
publisher = "MDPI",
journal = "Medicina (Lithuania)",
title = "Impact of tree pollen distribution on allergic diseases in serbia: Evidence of implementation of allergen immunotherapy to Betula verrucosa",
volume = "56",
number = "2",
doi = "10.3390/medicina56020059"
}
Minić, R., Josipović, M., Tomić Spirić, V., Gavrović-Jankulović, M., Perić Popadić, A., Prokopijević, I., Ljubičić, A., Stamenković, D.,& Burazer, L. M. (2020). Impact of tree pollen distribution on allergic diseases in serbia: Evidence of implementation of allergen immunotherapy to Betula verrucosa.
Medicina (Lithuania)
MDPI., 56(2).
https://doi.org/10.3390/medicina56020059
Minić R, Josipović M, Tomić Spirić V, Gavrović-Jankulović M, Perić Popadić A, Prokopijević I, Ljubičić A, Stamenković D, Burazer LM. Impact of tree pollen distribution on allergic diseases in serbia: Evidence of implementation of allergen immunotherapy to Betula verrucosa. Medicina (Lithuania). 2020;56(2)
Minić Rajna, Josipović Mirjana, Tomić Spirić Vesna, Gavrović-Jankulović Marija, Perić Popadić Aleksandra, Prokopijević Ivana, Ljubičić Ana, Stamenković Danijela, Burazer Lidija M., "Impact of tree pollen distribution on allergic diseases in serbia: Evidence of implementation of allergen immunotherapy to Betula verrucosa" Medicina (Lithuania), 56, no. 2 (2020),
https://doi.org/10.3390/medicina56020059 .
1
1
1
1

Disposable Biosensor Based on Amidase/CeO2/GNR Modified Inkjet-printed CNT Electrodes-droplet Based Paracetamol Detection in Biological Fluids for “Point-of-care” Applications

Stanković, Dalibor; Ognjanović, Miloš; Jović, Milica; Cuplić, Valentina; Lesch, Andreas; Girault, Hubert H.; Gavrović-Jankulović, Marija; Antić, Bratislav

(Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, 2019)

TY  - JOUR
AU  - Stanković, Dalibor
AU  - Ognjanović, Miloš
AU  - Jović, Milica
AU  - Cuplić, Valentina
AU  - Lesch, Andreas
AU  - Girault, Hubert H.
AU  - Gavrović-Jankulović, Marija
AU  - Antić, Bratislav
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3718
AB  - A disposable acetaminophen biosensor based on inkjet-printed CNT electrodes (IJPCNT) modified with amidase/cerium dioxide@graphene nanoribbons composite was developed (ACeO2@GNR/IJPCNT). The enzyme amidase A was used for the first time as a recognition element. Inkjet-printed CNT electrodes served as a basis for the construction of a biosensor that enables droplet detection using 5 μL sample volume. The biosensor showed high selectivity, sensitivity, a low detection limit of 0.18 μM and a wide working linear range from 1 to 100 μM. The proposed approach allows fast and reliable detection of acetaminophen in biological fluids with negligible matrix effect and remarkable reproducibility.
PB  - Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
T2  - Electroanalysis
T1  - Disposable Biosensor Based on Amidase/CeO2/GNR Modified Inkjet-printed CNT Electrodes-droplet Based Paracetamol Detection in Biological Fluids for “Point-of-care” Applications
VL  - 31
IS  - 8
SP  - 1534
EP  - 1542
DO  - 10.1002/elan.201900129
ER  - 
@article{
author = "Stanković, Dalibor and Ognjanović, Miloš and Jović, Milica and Cuplić, Valentina and Lesch, Andreas and Girault, Hubert H. and Gavrović-Jankulović, Marija and Antić, Bratislav",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3718",
abstract = "A disposable acetaminophen biosensor based on inkjet-printed CNT electrodes (IJPCNT) modified with amidase/cerium dioxide@graphene nanoribbons composite was developed (ACeO2@GNR/IJPCNT). The enzyme amidase A was used for the first time as a recognition element. Inkjet-printed CNT electrodes served as a basis for the construction of a biosensor that enables droplet detection using 5 μL sample volume. The biosensor showed high selectivity, sensitivity, a low detection limit of 0.18 μM and a wide working linear range from 1 to 100 μM. The proposed approach allows fast and reliable detection of acetaminophen in biological fluids with negligible matrix effect and remarkable reproducibility.",
publisher = "Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",
journal = "Electroanalysis",
title = "Disposable Biosensor Based on Amidase/CeO2/GNR Modified Inkjet-printed CNT Electrodes-droplet Based Paracetamol Detection in Biological Fluids for “Point-of-care” Applications",
volume = "31",
number = "8",
pages = "1534-1542",
doi = "10.1002/elan.201900129"
}
Stanković, D., Ognjanović, M., Jović, M., Cuplić, V., Lesch, A., Girault, H. H., Gavrović-Jankulović, M.,& Antić, B. (2019). Disposable Biosensor Based on Amidase/CeO2/GNR Modified Inkjet-printed CNT Electrodes-droplet Based Paracetamol Detection in Biological Fluids for “Point-of-care” Applications.
Electroanalysis
Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim., 31(8), 1534-1542.
https://doi.org/10.1002/elan.201900129
Stanković D, Ognjanović M, Jović M, Cuplić V, Lesch A, Girault HH, Gavrović-Jankulović M, Antić B. Disposable Biosensor Based on Amidase/CeO2/GNR Modified Inkjet-printed CNT Electrodes-droplet Based Paracetamol Detection in Biological Fluids for “Point-of-care” Applications. Electroanalysis. 2019;31(8):1534-1542
Stanković Dalibor, Ognjanović Miloš, Jović Milica, Cuplić Valentina, Lesch Andreas, Girault Hubert H., Gavrović-Jankulović Marija, Antić Bratislav, "Disposable Biosensor Based on Amidase/CeO2/GNR Modified Inkjet-printed CNT Electrodes-droplet Based Paracetamol Detection in Biological Fluids for “Point-of-care” Applications" Electroanalysis, 31, no. 8 (2019):1534-1542,
https://doi.org/10.1002/elan.201900129 .
6
4
6

Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases

Stojsavljević, Aleksandar; Rovčanin, Branislav; Krstić, Đurđa D.; Borković-Mitić, Slavica S.; Paunović, Ivan; Kodranov, Igor D.; Gavrović-Jankulović, Marija; Manojlović, Dragan D.

(Elsevier, 2019)

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa D.
AU  - Borković-Mitić, Slavica S.
AU  - Paunović, Ivan
AU  - Kodranov, Igor D.
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan D.
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3285
AB  - Evaluation of trace metals at level of solid tissue can provide better information than blood or urine and, therefore, could highlight the role of metals in the etiology of organ-specific disease. The current study aimed to establish the baseline content of four essential (Mn, Cu, Zn, Se) and four toxic metals (As, Cd, Pb, U) in the healthy thyroid tissues (HTTs) by considering sex, age and smoking habits. A further aim was to examine whether differences in the content of metals exist in regard to the thyroid diseases, such as benign tumor (BT), Hashimoto's thyroiditis (HT), multinodular goiter (MNG) and thyroid cancer (TC). A total number of investigated tissue samples were 423. All metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). It was found that the content of Cu and U was higher in HTTs of women, while the content of Zn was higher in HTTs of men. Increased content of Zn and decreased content of U was found in the group of HTTs above 50 years compared to a younger group (<50 years). Increased content of Cd, Pb and U distinguish smokers from the non-smokers. In comparison with other population groups worldwide, investigated Serbian population had up to 15 times reduced content of Se. Despite the difference in metal's profile according to biological variables, this study also demonstrated, for the first time, that each thyroid disease has its unique metal's profile. The most altered metal's content was found in tissues with HT. Contrarily, the greatest similarity in metal's content with HTTs was found in BT tissues. Based on the increased content, metal's that dominantly discriminated HTTs from the HT, MNG and TC was As, Pb and Cd, respectively. Reported results could highlight the role of toxic and essential trace metals in the not very well clarified etiology of thyroid diseases and, moreover, could provide a molecular basis for pathophysiological changes of metal's hazardous effects on thyroid health at the tissue level.
PB  - Elsevier
T2  - Ecotoxicology and Environmental Safety
T1  - Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases
VL  - 183
DO  - 10.1016/j.ecoenv.2019.109479
ER  - 
@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa D. and Borković-Mitić, Slavica S. and Paunović, Ivan and Kodranov, Igor D. and Gavrović-Jankulović, Marija and Manojlović, Dragan D.",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3285",
abstract = "Evaluation of trace metals at level of solid tissue can provide better information than blood or urine and, therefore, could highlight the role of metals in the etiology of organ-specific disease. The current study aimed to establish the baseline content of four essential (Mn, Cu, Zn, Se) and four toxic metals (As, Cd, Pb, U) in the healthy thyroid tissues (HTTs) by considering sex, age and smoking habits. A further aim was to examine whether differences in the content of metals exist in regard to the thyroid diseases, such as benign tumor (BT), Hashimoto's thyroiditis (HT), multinodular goiter (MNG) and thyroid cancer (TC). A total number of investigated tissue samples were 423. All metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). It was found that the content of Cu and U was higher in HTTs of women, while the content of Zn was higher in HTTs of men. Increased content of Zn and decreased content of U was found in the group of HTTs above 50 years compared to a younger group (<50 years). Increased content of Cd, Pb and U distinguish smokers from the non-smokers. In comparison with other population groups worldwide, investigated Serbian population had up to 15 times reduced content of Se. Despite the difference in metal's profile according to biological variables, this study also demonstrated, for the first time, that each thyroid disease has its unique metal's profile. The most altered metal's content was found in tissues with HT. Contrarily, the greatest similarity in metal's content with HTTs was found in BT tissues. Based on the increased content, metal's that dominantly discriminated HTTs from the HT, MNG and TC was As, Pb and Cd, respectively. Reported results could highlight the role of toxic and essential trace metals in the not very well clarified etiology of thyroid diseases and, moreover, could provide a molecular basis for pathophysiological changes of metal's hazardous effects on thyroid health at the tissue level.",
publisher = "Elsevier",
journal = "Ecotoxicology and Environmental Safety",
title = "Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases",
volume = "183",
doi = "10.1016/j.ecoenv.2019.109479"
}
Stojsavljević, A., Rovčanin, B., Krstić, Đ. D., Borković-Mitić, S. S., Paunović, I., Kodranov, I. D., Gavrović-Jankulović, M.,& Manojlović, D. D. (2019). Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases.
Ecotoxicology and Environmental Safety
Elsevier., 183.
https://doi.org/10.1016/j.ecoenv.2019.109479
Stojsavljević A, Rovčanin B, Krstić ĐD, Borković-Mitić SS, Paunović I, Kodranov ID, Gavrović-Jankulović M, Manojlović DD. Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases. Ecotoxicology and Environmental Safety. 2019;183
Stojsavljević Aleksandar, Rovčanin Branislav, Krstić Đurđa D., Borković-Mitić Slavica S., Paunović Ivan, Kodranov Igor D., Gavrović-Jankulović Marija, Manojlović Dragan D., "Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases" Ecotoxicology and Environmental Safety, 183 (2019),
https://doi.org/10.1016/j.ecoenv.2019.109479 .
1
14
9
13

Supplementary data for the article: Stojsavljević, A.; Rovčanin, B.; Krstić, Đ.; Borković-Mitić, S.; Paunović, I.; Kodranov, I.; Gavrović-Jankulović, M.; Manojlović, D. Evaluation of Trace Metals in Thyroid Tissues: Comparative Analysis with Benign and Malignant Thyroid Diseases. Ecotoxicology and Environmental Safety 2019, 183. https://doi.org/10.1016/j.ecoenv.2019.109479

Stojsavljević, Aleksandar; Rovčanin, Branislav; Krstić, Đurđa D.; Borković-Mitić, Slavica S.; Paunović, Ivan; Kodranov, Igor D.; Gavrović-Jankulović, Marija; Manojlović, Dragan D.

(Elsevier, 2019)

TY  - BOOK
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa D.
AU  - Borković-Mitić, Slavica S.
AU  - Paunović, Ivan
AU  - Kodranov, Igor D.
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan D.
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3286
PB  - Elsevier
T2  - Ecotoxicology and Environmental Safety
T1  - Supplementary data for the article: Stojsavljević, A.; Rovčanin, B.; Krstić, Đ.; Borković-Mitić, S.; Paunović, I.; Kodranov, I.; Gavrović-Jankulović, M.; Manojlović, D. Evaluation of  Trace Metals in Thyroid Tissues: Comparative Analysis with Benign and Malignant Thyroid Diseases. Ecotoxicology and Environmental Safety 2019, 183. https://doi.org/10.1016/j.ecoenv.2019.109479
ER  - 
@book{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa D. and Borković-Mitić, Slavica S. and Paunović, Ivan and Kodranov, Igor D. and Gavrović-Jankulović, Marija and Manojlović, Dragan D.",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3286",
publisher = "Elsevier",
journal = "Ecotoxicology and Environmental Safety",
title = "Supplementary data for the article: Stojsavljević, A.; Rovčanin, B.; Krstić, Đ.; Borković-Mitić, S.; Paunović, I.; Kodranov, I.; Gavrović-Jankulović, M.; Manojlović, D. Evaluation of  Trace Metals in Thyroid Tissues: Comparative Analysis with Benign and Malignant Thyroid Diseases. Ecotoxicology and Environmental Safety 2019, 183. https://doi.org/10.1016/j.ecoenv.2019.109479"
}
Stojsavljević, A., Rovčanin, B., Krstić, Đ. D., Borković-Mitić, S. S., Paunović, I., Kodranov, I. D., Gavrović-Jankulović, M.,& Manojlović, D. D. (2019). Supplementary data for the article: Stojsavljević, A.; Rovčanin, B.; Krstić, Đ.; Borković-Mitić, S.; Paunović, I.; Kodranov, I.; Gavrović-Jankulović, M.; Manojlović, D. Evaluation of  Trace Metals in Thyroid Tissues: Comparative Analysis with Benign and Malignant Thyroid Diseases. Ecotoxicology and Environmental Safety 2019, 183. https://doi.org/10.1016/j.ecoenv.2019.109479.
Ecotoxicology and Environmental Safety
Elsevier..
Stojsavljević A, Rovčanin B, Krstić ĐD, Borković-Mitić SS, Paunović I, Kodranov ID, Gavrović-Jankulović M, Manojlović DD. Supplementary data for the article: Stojsavljević, A.; Rovčanin, B.; Krstić, Đ.; Borković-Mitić, S.; Paunović, I.; Kodranov, I.; Gavrović-Jankulović, M.; Manojlović, D. Evaluation of  Trace Metals in Thyroid Tissues: Comparative Analysis with Benign and Malignant Thyroid Diseases. Ecotoxicology and Environmental Safety 2019, 183. https://doi.org/10.1016/j.ecoenv.2019.109479. Ecotoxicology and Environmental Safety. 2019;
Stojsavljević Aleksandar, Rovčanin Branislav, Krstić Đurđa D., Borković-Mitić Slavica S., Paunović Ivan, Kodranov Igor D., Gavrović-Jankulović Marija, Manojlović Dragan D., "Supplementary data for the article: Stojsavljević, A.; Rovčanin, B.; Krstić, Đ.; Borković-Mitić, S.; Paunović, I.; Kodranov, I.; Gavrović-Jankulović, M.; Manojlović, D. Evaluation of  Trace Metals in Thyroid Tissues: Comparative Analysis with Benign and Malignant Thyroid Diseases. Ecotoxicology and Environmental Safety 2019, 183. https://doi.org/10.1016/j.ecoenv.2019.109479" Ecotoxicology and Environmental Safety (2019)

Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology

Stojsavljević, Aleksandar; Rovčanin, Branislav; Krstić, Đurđa D.; Jagodić, Jovana; Borković-Mitić, Slavica S.; Paunović, Ivan; Živaljević, Vladan; Mitić, Bojan M.; Gavrović-Jankulović, Marija; Manojlović, Dragan D.

(Elsevier, 2019)

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa D.
AU  - Jagodić, Jovana
AU  - Borković-Mitić, Slavica S.
AU  - Paunović, Ivan
AU  - Živaljević, Vladan
AU  - Mitić, Bojan M.
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan D.
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3293
AB  - Background: The etiology of papillary thyroid carcinoma (PTC) is unknown and some literature data support the hypothesis that heavy metals, as endocrine disrupters, could play a major role in the pathogenesis of thyroid cancer. This study aimed to estimate the content of selected toxic and essential trace metals (Mn, Co, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U), as well as the selected ratio's (Cu/Zn and Cd/Se) in the malignant thyroid tissues according to sex, age, smoking habits, familial history of any thyroid disease, pathohistological (PH) types of PTC, tumor size, the existence of a thyroid capsular invasion, intrathyroid tumor dissemination, retrosternal thyroid growth, and TNM progress of PTC. Methods: The study included 66 patients with PTC (women/men ratio = 46/20, mean age: 54 ± 14 years). A comparative analysis was made by collecting the healthy thyroid tissues (HTTs) of the same patients, making the total number of samples 132. All trace metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). Results: Metals that significantly separated papillary thyroid tissues (PTTs) from the HTTs were Cd, U and Se (p < 0.05). The obtained negative correlation between Cd and Se in the PTTs could explain extrusion of essential Se caused by increased content of Cd. Only Cd had an influence on the retrosternal thyroid growth, while the essential metals (Mn, Co, and Zn) had an influence on thyroid capsular invasion. Conclusion: It was found that Cd act as the main endocrine disrupter, which could highlight its role in the etiology of PTC. Considering that the Cd/Se ratio significantly separated two studied groups and had an influence on the retrosternal thyroid growth, its altered content could contribute to the better understanding of the molecular basis for pathophysiological changes in the PTC.
PB  - Elsevier
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology
VL  - 55
SP  - 190
EP  - 195
DO  - 10.1016/j.jtemb.2019.06.009
ER  - 
@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa D. and Jagodić, Jovana and Borković-Mitić, Slavica S. and Paunović, Ivan and Živaljević, Vladan and Mitić, Bojan M. and Gavrović-Jankulović, Marija and Manojlović, Dragan D.",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3293",
abstract = "Background: The etiology of papillary thyroid carcinoma (PTC) is unknown and some literature data support the hypothesis that heavy metals, as endocrine disrupters, could play a major role in the pathogenesis of thyroid cancer. This study aimed to estimate the content of selected toxic and essential trace metals (Mn, Co, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U), as well as the selected ratio's (Cu/Zn and Cd/Se) in the malignant thyroid tissues according to sex, age, smoking habits, familial history of any thyroid disease, pathohistological (PH) types of PTC, tumor size, the existence of a thyroid capsular invasion, intrathyroid tumor dissemination, retrosternal thyroid growth, and TNM progress of PTC. Methods: The study included 66 patients with PTC (women/men ratio = 46/20, mean age: 54 ± 14 years). A comparative analysis was made by collecting the healthy thyroid tissues (HTTs) of the same patients, making the total number of samples 132. All trace metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). Results: Metals that significantly separated papillary thyroid tissues (PTTs) from the HTTs were Cd, U and Se (p < 0.05). The obtained negative correlation between Cd and Se in the PTTs could explain extrusion of essential Se caused by increased content of Cd. Only Cd had an influence on the retrosternal thyroid growth, while the essential metals (Mn, Co, and Zn) had an influence on thyroid capsular invasion. Conclusion: It was found that Cd act as the main endocrine disrupter, which could highlight its role in the etiology of PTC. Considering that the Cd/Se ratio significantly separated two studied groups and had an influence on the retrosternal thyroid growth, its altered content could contribute to the better understanding of the molecular basis for pathophysiological changes in the PTC.",
publisher = "Elsevier",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology",
volume = "55",
pages = "190-195",
doi = "10.1016/j.jtemb.2019.06.009"
}
Stojsavljević, A., Rovčanin, B., Krstić, Đ. D., Jagodić, J., Borković-Mitić, S. S., Paunović, I., Živaljević, V., Mitić, B. M., Gavrović-Jankulović, M.,& Manojlović, D. D. (2019). Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology.
Journal of Trace Elements in Medicine and Biology
Elsevier., 55, 190-195.
https://doi.org/10.1016/j.jtemb.2019.06.009
Stojsavljević A, Rovčanin B, Krstić ĐD, Jagodić J, Borković-Mitić SS, Paunović I, Živaljević V, Mitić BM, Gavrović-Jankulović M, Manojlović DD. Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology. Journal of Trace Elements in Medicine and Biology. 2019;55:190-195
Stojsavljević Aleksandar, Rovčanin Branislav, Krstić Đurđa D., Jagodić Jovana, Borković-Mitić Slavica S., Paunović Ivan, Živaljević Vladan, Mitić Bojan M., Gavrović-Jankulović Marija, Manojlović Dragan D., "Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology" Journal of Trace Elements in Medicine and Biology, 55 (2019):190-195,
https://doi.org/10.1016/j.jtemb.2019.06.009 .
1
13
5
13

Supplementary material for the article: Stojsavljević, A.; Rovčanin, B.; Krstić; Jagodić, J.; Borković-Mitić, S.; Paunović, I.; Živaljević, V.; Mitić, B.; Gavrović-Jankulović, M.; Manojlović, D. Cadmium as Main Endocrine Disruptor in Papillary Thyroid Carcinoma and the Significance of Cd/Se Ratio for Thyroid Tissue Pathophysiology. Journal of Trace Elements in Medicine and Biology 2019, 55, 190–195. https://doi.org/10.1016/j.jtemb.2019.06.009

Stojsavljević, Aleksandar; Rovčanin, Branislav; Krstić, Đurđa D.; Jagodić, Jovana; Borković-Mitić, Slavica S.; Paunović, Ivan; Živaljević, Vladan; Mitić, Bojan M.; Gavrović-Jankulović, Marija; Manojlović, Dragan D.

(Elsevier, 2019)

TY  - BOOK
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa D.
AU  - Jagodić, Jovana
AU  - Borković-Mitić, Slavica S.
AU  - Paunović, Ivan
AU  - Živaljević, Vladan
AU  - Mitić, Bojan M.
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan D.
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3294
PB  - Elsevier
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Supplementary material for the article: Stojsavljević, A.; Rovčanin, B.; Krstić; Jagodić, J.; Borković-Mitić, S.; Paunović, I.; Živaljević, V.; Mitić, B.; Gavrović-Jankulović, M.; Manojlović, D. Cadmium as Main Endocrine Disruptor in Papillary Thyroid Carcinoma and the Significance of Cd/Se Ratio for Thyroid Tissue Pathophysiology. Journal of Trace Elements in Medicine and Biology 2019, 55, 190–195. https://doi.org/10.1016/j.jtemb.2019.06.009
ER  - 
@book{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa D. and Jagodić, Jovana and Borković-Mitić, Slavica S. and Paunović, Ivan and Živaljević, Vladan and Mitić, Bojan M. and Gavrović-Jankulović, Marija and Manojlović, Dragan D.",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3294",
publisher = "Elsevier",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Supplementary material for the article: Stojsavljević, A.; Rovčanin, B.; Krstić; Jagodić, J.; Borković-Mitić, S.; Paunović, I.; Živaljević, V.; Mitić, B.; Gavrović-Jankulović, M.; Manojlović, D. Cadmium as Main Endocrine Disruptor in Papillary Thyroid Carcinoma and the Significance of Cd/Se Ratio for Thyroid Tissue Pathophysiology. Journal of Trace Elements in Medicine and Biology 2019, 55, 190–195. https://doi.org/10.1016/j.jtemb.2019.06.009"
}
Stojsavljević, A., Rovčanin, B., Krstić, Đ. D., Jagodić, J., Borković-Mitić, S. S., Paunović, I., Živaljević, V., Mitić, B. M., Gavrović-Jankulović, M.,& Manojlović, D. D. (2019). Supplementary material for the article: Stojsavljević, A.; Rovčanin, B.; Krstić; Jagodić, J.; Borković-Mitić, S.; Paunović, I.; Živaljević, V.; Mitić, B.; Gavrović-Jankulović, M.; Manojlović, D. Cadmium as Main Endocrine Disruptor in Papillary Thyroid Carcinoma and the Significance of Cd/Se Ratio for Thyroid Tissue Pathophysiology. Journal of Trace Elements in Medicine and Biology 2019, 55, 190–195. https://doi.org/10.1016/j.jtemb.2019.06.009.
Journal of Trace Elements in Medicine and Biology
Elsevier..
Stojsavljević A, Rovčanin B, Krstić ĐD, Jagodić J, Borković-Mitić SS, Paunović I, Živaljević V, Mitić BM, Gavrović-Jankulović M, Manojlović DD. Supplementary material for the article: Stojsavljević, A.; Rovčanin, B.; Krstić; Jagodić, J.; Borković-Mitić, S.; Paunović, I.; Živaljević, V.; Mitić, B.; Gavrović-Jankulović, M.; Manojlović, D. Cadmium as Main Endocrine Disruptor in Papillary Thyroid Carcinoma and the Significance of Cd/Se Ratio for Thyroid Tissue Pathophysiology. Journal of Trace Elements in Medicine and Biology 2019, 55, 190–195. https://doi.org/10.1016/j.jtemb.2019.06.009. Journal of Trace Elements in Medicine and Biology. 2019;
Stojsavljević Aleksandar, Rovčanin Branislav, Krstić Đurđa D., Jagodić Jovana, Borković-Mitić Slavica S., Paunović Ivan, Živaljević Vladan, Mitić Bojan M., Gavrović-Jankulović Marija, Manojlović Dragan D., "Supplementary material for the article: Stojsavljević, A.; Rovčanin, B.; Krstić; Jagodić, J.; Borković-Mitić, S.; Paunović, I.; Živaljević, V.; Mitić, B.; Gavrović-Jankulović, M.; Manojlović, D. Cadmium as Main Endocrine Disruptor in Papillary Thyroid Carcinoma and the Significance of Cd/Se Ratio for Thyroid Tissue Pathophysiology. Journal of Trace Elements in Medicine and Biology 2019, 55, 190–195. https://doi.org/10.1016/j.jtemb.2019.06.009" Journal of Trace Elements in Medicine and Biology (2019)

Hypersensitivity reactions to antiepileptic drugs in children

Atanasković-Marković, Marina; Janković, Jelena; Tmušić, Vladimir; Gavrović-Jankulović, Marija; Ćirković-Veličković, Tanja; Nikolić, Dimitrije; Škorić, Dejan

(John Wiley and Sons Ltd., 2019)

TY  - JOUR
AU  - Atanasković-Marković, Marina
AU  - Janković, Jelena
AU  - Tmušić, Vladimir
AU  - Gavrović-Jankulović, Marija
AU  - Ćirković-Veličković, Tanja
AU  - Nikolić, Dimitrije
AU  - Škorić, Dejan
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3704
AB  - Background: Antiepileptic drugs (AEDs) can cause hypersensitivity reactions in children. These reactions are mainly cutaneous, self-limiting, and benign, but life-threatening severe cutaneous adverse reactions can occur. Infections can lead to skin eruptions and mimic drug hypersensitivity reactions, if a drug is taken at the same time. The aims of our study were to confirm or rule out the diagnosis of hypersensitivity reactions to AEDs in children and to detect an infection which mimics these reactions. Methods: A prospective survey was conducted in a group of 100 children with histories of hypersensitivity reactions to AEDs by performing patch tests, delayed-reading intradermal test, and, in case of negative results, challenge test. In all children, a study was performed to detect infections by viruses or Mycoplasma pneumoniae. Results: Maculopapular exanthema and delayed-appearing urticaria were the most reported hypersensitivity reactions to AEDs. Sixty-six (66%) of 100 children had confirmed hypersensitivity reactions to AEDs. Fifty-nine children had positive patch test. No children had positive challenge tests. The most common AEDs causing hypersensitivity reactions were carbamazepine (45.4%) and lamotrigine (43.6%). Thirty-two children had positive tests for viruses or M pneumoniae, and nine of them had also a positive allergy work-up. Conclusion: Considering that there are no specific tests to distinguish between a viral infection and hypersensitivity reactions to AEDs in the acute phase, a diagnostic work-up should be performed in all children with suspected hypersensitivity reactions to AEDs, as well as infectious agent study, to remove a false label of hypersensitivity.
PB  - John Wiley and Sons Ltd.
T2  - Pediatric Allergy and Immunology
T1  - Hypersensitivity reactions to antiepileptic drugs in children
VL  - 30
IS  - 5
SP  - 547
EP  - 552
DO  - 10.1111/pai.13055
ER  - 
@article{
author = "Atanasković-Marković, Marina and Janković, Jelena and Tmušić, Vladimir and Gavrović-Jankulović, Marija and Ćirković-Veličković, Tanja and Nikolić, Dimitrije and Škorić, Dejan",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3704",
abstract = "Background: Antiepileptic drugs (AEDs) can cause hypersensitivity reactions in children. These reactions are mainly cutaneous, self-limiting, and benign, but life-threatening severe cutaneous adverse reactions can occur. Infections can lead to skin eruptions and mimic drug hypersensitivity reactions, if a drug is taken at the same time. The aims of our study were to confirm or rule out the diagnosis of hypersensitivity reactions to AEDs in children and to detect an infection which mimics these reactions. Methods: A prospective survey was conducted in a group of 100 children with histories of hypersensitivity reactions to AEDs by performing patch tests, delayed-reading intradermal test, and, in case of negative results, challenge test. In all children, a study was performed to detect infections by viruses or Mycoplasma pneumoniae. Results: Maculopapular exanthema and delayed-appearing urticaria were the most reported hypersensitivity reactions to AEDs. Sixty-six (66%) of 100 children had confirmed hypersensitivity reactions to AEDs. Fifty-nine children had positive patch test. No children had positive challenge tests. The most common AEDs causing hypersensitivity reactions were carbamazepine (45.4%) and lamotrigine (43.6%). Thirty-two children had positive tests for viruses or M pneumoniae, and nine of them had also a positive allergy work-up. Conclusion: Considering that there are no specific tests to distinguish between a viral infection and hypersensitivity reactions to AEDs in the acute phase, a diagnostic work-up should be performed in all children with suspected hypersensitivity reactions to AEDs, as well as infectious agent study, to remove a false label of hypersensitivity.",
publisher = "John Wiley and Sons Ltd.",
journal = "Pediatric Allergy and Immunology",
title = "Hypersensitivity reactions to antiepileptic drugs in children",
volume = "30",
number = "5",
pages = "547-552",
doi = "10.1111/pai.13055"
}
Atanasković-Marković, M., Janković, J., Tmušić, V., Gavrović-Jankulović, M., Ćirković-Veličković, T., Nikolić, D.,& Škorić, D. (2019). Hypersensitivity reactions to antiepileptic drugs in children.
Pediatric Allergy and Immunology
John Wiley and Sons Ltd.., 30(5), 547-552.
https://doi.org/10.1111/pai.13055
Atanasković-Marković M, Janković J, Tmušić V, Gavrović-Jankulović M, Ćirković-Veličković T, Nikolić D, Škorić D. Hypersensitivity reactions to antiepileptic drugs in children. Pediatric Allergy and Immunology. 2019;30(5):547-552
Atanasković-Marković Marina, Janković Jelena, Tmušić Vladimir, Gavrović-Jankulović Marija, Ćirković-Veličković Tanja, Nikolić Dimitrije, Škorić Dejan, "Hypersensitivity reactions to antiepileptic drugs in children" Pediatric Allergy and Immunology, 30, no. 5 (2019):547-552,
https://doi.org/10.1111/pai.13055 .
1
7
3
7

Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies

Lozano-Ojalvo, Daniel; Benedé, Sara; Antunes, Celia M.; Bavaro, Simona L.; Bouchaud, Grégory; Costa, Ana; Denery-Papini, Sandra; Díaz-Perales, Araceli; Garrido-Arandia, María; Gavrović-Jankulović, Marija; Hayen, Simone; Martínez-Blanco, Mónica; Molina, Elena; Monaci, Linda; Pieters, Raymond; Villemin, Clelia; Wichers, Harry J.; Wróblewska, Barbara; Willemsen, Linette E.M.; Roggen, Erwin L.; van Bilsen, Jolanda H.M.

(Elsevier, 2019)

TY  - JOUR
AU  - Lozano-Ojalvo, Daniel
AU  - Benedé, Sara
AU  - Antunes, Celia M.
AU  - Bavaro, Simona L.
AU  - Bouchaud, Grégory
AU  - Costa, Ana
AU  - Denery-Papini, Sandra
AU  - Díaz-Perales, Araceli
AU  - Garrido-Arandia, María
AU  - Gavrović-Jankulović, Marija
AU  - Hayen, Simone
AU  - Martínez-Blanco, Mónica
AU  - Molina, Elena
AU  - Monaci, Linda
AU  - Pieters, Raymond
AU  - Villemin, Clelia
AU  - Wichers, Harry J.
AU  - Wróblewska, Barbara
AU  - Willemsen, Linette E.M.
AU  - Roggen, Erwin L.
AU  - van Bilsen, Jolanda H.M.
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2864
AB  - Background: Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. Scope and approach: In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. Key findings and conclusions: The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches.
PB  - Elsevier
T2  - Trends in Food Science and Technology
T1  - Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies
VL  - 85
SP  - 307
EP  - 319
DO  - 10.1016/j.tifs.2019.01.014
ER  - 
@article{
author = "Lozano-Ojalvo, Daniel and Benedé, Sara and Antunes, Celia M. and Bavaro, Simona L. and Bouchaud, Grégory and Costa, Ana and Denery-Papini, Sandra and Díaz-Perales, Araceli and Garrido-Arandia, María and Gavrović-Jankulović, Marija and Hayen, Simone and Martínez-Blanco, Mónica and Molina, Elena and Monaci, Linda and Pieters, Raymond and Villemin, Clelia and Wichers, Harry J. and Wróblewska, Barbara and Willemsen, Linette E.M. and Roggen, Erwin L. and van Bilsen, Jolanda H.M.",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2864",
abstract = "Background: Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. Scope and approach: In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. Key findings and conclusions: The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches.",
publisher = "Elsevier",
journal = "Trends in Food Science and Technology",
title = "Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies",
volume = "85",
pages = "307-319",
doi = "10.1016/j.tifs.2019.01.014"
}
Lozano-Ojalvo, D., Benedé, S., Antunes, C. M., Bavaro, S. L., Bouchaud, G., Costa, A., Denery-Papini, S., Díaz-Perales, A., Garrido-Arandia, M., Gavrović-Jankulović, M., Hayen, S., Martínez-Blanco, M., Molina, E., Monaci, L., Pieters, R., Villemin, C., Wichers, H. J., Wróblewska, B., Willemsen, L. E.M., Roggen, E. L.,& van Bilsen, J. H.M. (2019). Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies.
Trends in Food Science and Technology
Elsevier., 85, 307-319.
https://doi.org/10.1016/j.tifs.2019.01.014
Lozano-Ojalvo D, Benedé S, Antunes CM, Bavaro SL, Bouchaud G, Costa A, Denery-Papini S, Díaz-Perales A, Garrido-Arandia M, Gavrović-Jankulović M, Hayen S, Martínez-Blanco M, Molina E, Monaci L, Pieters R, Villemin C, Wichers HJ, Wróblewska B, Willemsen LE, Roggen EL, van Bilsen JH. Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies. Trends in Food Science and Technology. 2019;85:307-319
Lozano-Ojalvo Daniel, Benedé Sara, Antunes Celia M., Bavaro Simona L., Bouchaud Grégory, Costa Ana, Denery-Papini Sandra, Díaz-Perales Araceli, Garrido-Arandia María, Gavrović-Jankulović Marija, Hayen Simone, Martínez-Blanco Mónica, Molina Elena, Monaci Linda, Pieters Raymond, Villemin Clelia, Wichers Harry J., Wróblewska Barbara, Willemsen Linette E.M., Roggen Erwin L., van Bilsen Jolanda H.M., "Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies" Trends in Food Science and Technology, 85 (2019):307-319,
https://doi.org/10.1016/j.tifs.2019.01.014 .
4
2
3

Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model

Nešić, Andrijana; Stam, Annemarie; Čavić, Milena; Ten Klooster, Jean Paul; Pieters, Raymond; Smit, Joost; Gavrović-Jankulović, Marija

(2019)

TY  - JOUR
AU  - Nešić, Andrijana
AU  - Stam, Annemarie
AU  - Čavić, Milena
AU  - Ten Klooster, Jean Paul
AU  - Pieters, Raymond
AU  - Smit, Joost
AU  - Gavrović-Jankulović, Marija
PY  - 2019
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85071845404&doi=10.1016%2fj.jff.2019.103556&partnerID=40&md5=c210fd8346babe18cc93e9b0994c4a1e
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3485
AB  - In this study, two intestinal models were employed to assess the modulatory potential of a major kiwifruit allergen on the innate immunity of epithelial cells. Effects of Act d 1 were analyzed in terms of gene expression and structural changes of tight junction (TJ) proteins, as well as up-regulation of pro-inflammatory cytokines in Caco-2 cells and, for the first time, in mouse-derived intestinal 2-dimensional (2D) organoids.

Biologically active Act d 1 induced up-regulation of TJ genes for CLDN-2, CLDN-3, CLDN-4, ZO-1, and on the protein level induced release of pro-inflammatory cytokines IL-1β, TNFα and IL-33 in both employed model systems. In 2D-organoids, active Act d 1 impaired the TJ protein networks of E-cadherin, claudin-3, and ZO-1.

2D-organoids generated from mouse intestine are a promising new model system for the assessment of allergen-induced intestinal cell responses and a useful tool for mitigation of risks associated with novel food proteins.
T2  - Journal of Functional Foods
T1  - Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model
VL  - 62
DO  - 10.1016/j.jff.2019.103556
ER  - 
@article{
author = "Nešić, Andrijana and Stam, Annemarie and Čavić, Milena and Ten Klooster, Jean Paul and Pieters, Raymond and Smit, Joost and Gavrović-Jankulović, Marija",
year = "2019",
url = "https://www.scopus.com/inward/record.uri?eid=2-s2.0-85071845404&doi=10.1016%2fj.jff.2019.103556&partnerID=40&md5=c210fd8346babe18cc93e9b0994c4a1e, http://cherry.chem.bg.ac.rs/handle/123456789/3485",
abstract = "In this study, two intestinal models were employed to assess the modulatory potential of a major kiwifruit allergen on the innate immunity of epithelial cells. Effects of Act d 1 were analyzed in terms of gene expression and structural changes of tight junction (TJ) proteins, as well as up-regulation of pro-inflammatory cytokines in Caco-2 cells and, for the first time, in mouse-derived intestinal 2-dimensional (2D) organoids.

Biologically active Act d 1 induced up-regulation of TJ genes for CLDN-2, CLDN-3, CLDN-4, ZO-1, and on the protein level induced release of pro-inflammatory cytokines IL-1β, TNFα and IL-33 in both employed model systems. In 2D-organoids, active Act d 1 impaired the TJ protein networks of E-cadherin, claudin-3, and ZO-1.

2D-organoids generated from mouse intestine are a promising new model system for the assessment of allergen-induced intestinal cell responses and a useful tool for mitigation of risks associated with novel food proteins.",
journal = "Journal of Functional Foods",
title = "Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model",
volume = "62",
doi = "10.1016/j.jff.2019.103556"
}
Nešić, A., Stam, A., Čavić, M., Ten Klooster, J. P., Pieters, R., Smit, J.,& Gavrović-Jankulović, M. (2019). Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model.
Journal of Functional Foods, 62.
https://doi.org/10.1016/j.jff.2019.103556
Nešić A, Stam A, Čavić M, Ten Klooster JP, Pieters R, Smit J, Gavrović-Jankulović M. Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model. Journal of Functional Foods. 2019;62
Nešić Andrijana, Stam Annemarie, Čavić Milena, Ten Klooster Jean Paul, Pieters Raymond, Smit Joost, Gavrović-Jankulović Marija, "Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model" Journal of Functional Foods, 62 (2019),
https://doi.org/10.1016/j.jff.2019.103556 .
2
1
2

A real-life study of the efficacy of sublingual immunotherapy against weed allergies in the Serbian population

Tadić, D.; Popović, Milica; Gavrović-Jankulović, Marija; Đurić, Vojislav; Tomić-Špirić, Vesna; Rašković, Sanvila S.; Perić Popadić, Aleksandra

(Elsevier, 2019)

TY  - JOUR
AU  - Tadić, D.
AU  - Popović, Milica
AU  - Gavrović-Jankulović, Marija
AU  - Đurić, Vojislav
AU  - Tomić-Špirić, Vesna
AU  - Rašković, Sanvila S.
AU  - Perić Popadić, Aleksandra
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3728
AB  - Introduction: In the Serbian population allergy to weed pollen is the most common type of pollen-associated allergy, ahead of grass and tree pollens. Besides causing discomfort, allergy to pollen is often associated with rhinitis and asthma. Allergen-specific immunotherapy (ASIT) is the only treatment that can lead to potential long-term immune modification while reducing development of new sensitization and halting disease progression. The aim of the present study is to evaluate the efficacy of sublingual immunotherapy (SLIT) to weeds in the adult patient population using vaccine produced by the local Serbian Torlak Institute for virology, vaccines and serum. Methods: Adult patients with a clinical history of allergic rhinitis with and without asthma were included in the study. IgE-mediated sensitization to grass, tree and weed pollens was confirmed by skin prick testing and/or positive specific IgE. Patients were divided into two groups: patients with allergy to tree and grass pollen and patients with allergy to weeds. All patients received SLIT for three years, either with or without additional symptomatic therapy. Results: Three-year SLIT therapy led to significant improvement in several parameters, including skin-prick reactivity, decrease in specific IgE and use of symptomatic therapy, with mild adverse effects and high patient satisfaction concerning therapy. Conclusion: Three-year SLIT is a safe and efficient treatment option for respiratory allergy to weeds. Further observations in a larger number of patients could provide a better epidemiological evaluation of SLIT, but the positive effects we observed in our study may be considered representative despite the small number of patients.
PB  - Elsevier
T2  - Revue Francaise d'Allergologie
T1  - A real-life study of the efficacy of sublingual immunotherapy against weed allergies in the Serbian population
VL  - 59
IS  - 7
SP  - 474
EP  - 480
DO  - 10.1016/j.reval.2019.05.006
ER  - 
@article{
author = "Tadić, D. and Popović, Milica and Gavrović-Jankulović, Marija and Đurić, Vojislav and Tomić-Špirić, Vesna and Rašković, Sanvila S. and Perić Popadić, Aleksandra",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3728",
abstract = "Introduction: In the Serbian population allergy to weed pollen is the most common type of pollen-associated allergy, ahead of grass and tree pollens. Besides causing discomfort, allergy to pollen is often associated with rhinitis and asthma. Allergen-specific immunotherapy (ASIT) is the only treatment that can lead to potential long-term immune modification while reducing development of new sensitization and halting disease progression. The aim of the present study is to evaluate the efficacy of sublingual immunotherapy (SLIT) to weeds in the adult patient population using vaccine produced by the local Serbian Torlak Institute for virology, vaccines and serum. Methods: Adult patients with a clinical history of allergic rhinitis with and without asthma were included in the study. IgE-mediated sensitization to grass, tree and weed pollens was confirmed by skin prick testing and/or positive specific IgE. Patients were divided into two groups: patients with allergy to tree and grass pollen and patients with allergy to weeds. All patients received SLIT for three years, either with or without additional symptomatic therapy. Results: Three-year SLIT therapy led to significant improvement in several parameters, including skin-prick reactivity, decrease in specific IgE and use of symptomatic therapy, with mild adverse effects and high patient satisfaction concerning therapy. Conclusion: Three-year SLIT is a safe and efficient treatment option for respiratory allergy to weeds. Further observations in a larger number of patients could provide a better epidemiological evaluation of SLIT, but the positive effects we observed in our study may be considered representative despite the small number of patients.",
publisher = "Elsevier",
journal = "Revue Francaise d'Allergologie",
title = "A real-life study of the efficacy of sublingual immunotherapy against weed allergies in the Serbian population",
volume = "59",
number = "7",
pages = "474-480",
doi = "10.1016/j.reval.2019.05.006"
}
Tadić, D., Popović, M., Gavrović-Jankulović, M., Đurić, V., Tomić-Špirić, V., Rašković, S. S.,& Perić Popadić, A. (2019). A real-life study of the efficacy of sublingual immunotherapy against weed allergies in the Serbian population.
Revue Francaise d'Allergologie
Elsevier., 59(7), 474-480.
https://doi.org/10.1016/j.reval.2019.05.006
Tadić D, Popović M, Gavrović-Jankulović M, Đurić V, Tomić-Špirić V, Rašković SS, Perić Popadić A. A real-life study of the efficacy of sublingual immunotherapy against weed allergies in the Serbian population. Revue Francaise d'Allergologie. 2019;59(7):474-480
Tadić D., Popović Milica, Gavrović-Jankulović Marija, Đurić Vojislav, Tomić-Špirić Vesna, Rašković Sanvila S., Perić Popadić Aleksandra, "A real-life study of the efficacy of sublingual immunotherapy against weed allergies in the Serbian population" Revue Francaise d'Allergologie, 59, no. 7 (2019):474-480,
https://doi.org/10.1016/j.reval.2019.05.006 .

The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines

Nešić, Andrijana N.; Čavić, Milena; Popović, Milica; Zlatanova, Milena; Pieters, Raymond; Smit, Joost; Gavrović-Jankulović, Marija

(MDPI, 2019)

TY  - JOUR
AU  - Nešić, Andrijana N.
AU  - Čavić, Milena
AU  - Popović, Milica
AU  - Zlatanova, Milena
AU  - Pieters, Raymond
AU  - Smit, Joost
AU  - Gavrović-Jankulović, Marija
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3799
AB  - Impairment of the intestinal barrier is one of the key events in the initiation of the sensitization process in food allergy. The aim of this study was to explore the effects of kiwifruit allergen Act d 1 on intestinal permeability and tight junction protein (TJP) gene expression in vivo and to explore its potential to activate the NF-ĸB signaling pathway and to regulate expression of epithelial pro-allergenic cytokines. Influences of Act d 1 on TJP gene expression and pro-allergenic cytokines in the mouse intestine was analyzed by qPCR upon allergen administration by oral gavage. The effect on the in vivo intestinal permeability was assessed in ELISA by measuring the translocation of β-lactoglobulin (BLG) into circulation. The capacity of Act d 1 to activate the NF-ĸB pathway was tested in HEK293 cells by fluorescent microscopy and flow cytometry. Administration of Actinidin (Act d 1) increased intestinal permeability to the BLG. This was accompanied by changes in gene expression of TJP mRNA and pro-allergenic cytokines IL-25, IL-33, and thymic stromal lymphopoietin (TSLP) compared to the control. Act d 1 reduced TEER of the HEK293 monolayer, was positive in an NF-ĸB-reporter HEK293 cell assay, and induced secretion of TSLP. These findings shed more light on the molecular events in the sensitization process of kiwifruit but possibly also of other protease food allergens.
PB  - MDPI
T2  - Biomolecules
T1  - The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines
VL  - 9
IS  - 12
SP  - 816
DO  - 10.3390/biom9120816
ER  - 
@article{
author = "Nešić, Andrijana N. and Čavić, Milena and Popović, Milica and Zlatanova, Milena and Pieters, Raymond and Smit, Joost and Gavrović-Jankulović, Marija",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3799",
abstract = "Impairment of the intestinal barrier is one of the key events in the initiation of the sensitization process in food allergy. The aim of this study was to explore the effects of kiwifruit allergen Act d 1 on intestinal permeability and tight junction protein (TJP) gene expression in vivo and to explore its potential to activate the NF-ĸB signaling pathway and to regulate expression of epithelial pro-allergenic cytokines. Influences of Act d 1 on TJP gene expression and pro-allergenic cytokines in the mouse intestine was analyzed by qPCR upon allergen administration by oral gavage. The effect on the in vivo intestinal permeability was assessed in ELISA by measuring the translocation of β-lactoglobulin (BLG) into circulation. The capacity of Act d 1 to activate the NF-ĸB pathway was tested in HEK293 cells by fluorescent microscopy and flow cytometry. Administration of Actinidin (Act d 1) increased intestinal permeability to the BLG. This was accompanied by changes in gene expression of TJP mRNA and pro-allergenic cytokines IL-25, IL-33, and thymic stromal lymphopoietin (TSLP) compared to the control. Act d 1 reduced TEER of the HEK293 monolayer, was positive in an NF-ĸB-reporter HEK293 cell assay, and induced secretion of TSLP. These findings shed more light on the molecular events in the sensitization process of kiwifruit but possibly also of other protease food allergens.",
publisher = "MDPI",
journal = "Biomolecules",
title = "The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines",
volume = "9",
number = "12",
pages = "816",
doi = "10.3390/biom9120816"
}
Nešić, A. N., Čavić, M., Popović, M., Zlatanova, M., Pieters, R., Smit, J.,& Gavrović-Jankulović, M. (2019). The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines.
Biomolecules
MDPI., 9(12), 816.
https://doi.org/10.3390/biom9120816
Nešić AN, Čavić M, Popović M, Zlatanova M, Pieters R, Smit J, Gavrović-Jankulović M. The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines. Biomolecules. 2019;9(12):816
Nešić Andrijana N., Čavić Milena, Popović Milica, Zlatanova Milena, Pieters Raymond, Smit Joost, Gavrović-Jankulović Marija, "The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines" Biomolecules, 9, no. 12 (2019):816,
https://doi.org/10.3390/biom9120816 .
1
3
3

Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies

Lozano-Ojalvo, Daniel; Benedé, Sara; Antunes, Celia M.; Bavaro, Simona L.; Bouchaud, Grégory; Costa, Ana; Denery-Papini, Sandra; Díaz-Perales, Araceli; Garrido-Arandia, María; Gavrović-Jankulović, Marija; Hayen, Simone; Martínez-Blanco, Mónica; Molina, Elena; Monaci, Linda; Pieters, Raymond; Villemin, Clelia; Wichers, Harry J.; Wróblewska, Barbara; Willemsen, Linette E.M.; Roggen, Erwin L.; van Bilsen, Jolanda H.M.

(Elsevier, 2019)

TY  - JOUR
AU  - Lozano-Ojalvo, Daniel
AU  - Benedé, Sara
AU  - Antunes, Celia M.
AU  - Bavaro, Simona L.
AU  - Bouchaud, Grégory
AU  - Costa, Ana
AU  - Denery-Papini, Sandra
AU  - Díaz-Perales, Araceli
AU  - Garrido-Arandia, María
AU  - Gavrović-Jankulović, Marija
AU  - Hayen, Simone
AU  - Martínez-Blanco, Mónica
AU  - Molina, Elena
AU  - Monaci, Linda
AU  - Pieters, Raymond
AU  - Villemin, Clelia
AU  - Wichers, Harry J.
AU  - Wróblewska, Barbara
AU  - Willemsen, Linette E.M.
AU  - Roggen, Erwin L.
AU  - van Bilsen, Jolanda H.M.
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2852
AB  - Background: Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. Scope and approach: In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. Key findings and conclusions: The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches.
PB  - Elsevier
T2  - Trends in Food Science and Technology
T1  - Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies
VL  - 85
SP  - 307
EP  - 319
DO  - 10.1016/j.tifs.2019.01.014
ER  - 
@article{
author = "Lozano-Ojalvo, Daniel and Benedé, Sara and Antunes, Celia M. and Bavaro, Simona L. and Bouchaud, Grégory and Costa, Ana and Denery-Papini, Sandra and Díaz-Perales, Araceli and Garrido-Arandia, María and Gavrović-Jankulović, Marija and Hayen, Simone and Martínez-Blanco, Mónica and Molina, Elena and Monaci, Linda and Pieters, Raymond and Villemin, Clelia and Wichers, Harry J. and Wróblewska, Barbara and Willemsen, Linette E.M. and Roggen, Erwin L. and van Bilsen, Jolanda H.M.",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2852",
abstract = "Background: Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. Scope and approach: In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. Key findings and conclusions: The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches.",
publisher = "Elsevier",
journal = "Trends in Food Science and Technology",
title = "Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies",
volume = "85",
pages = "307-319",
doi = "10.1016/j.tifs.2019.01.014"
}
Lozano-Ojalvo, D., Benedé, S., Antunes, C. M., Bavaro, S. L., Bouchaud, G., Costa, A., Denery-Papini, S., Díaz-Perales, A., Garrido-Arandia, M., Gavrović-Jankulović, M., Hayen, S., Martínez-Blanco, M., Molina, E., Monaci, L., Pieters, R., Villemin, C., Wichers, H. J., Wróblewska, B., Willemsen, L. E.M., Roggen, E. L.,& van Bilsen, J. H.M. (2019). Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies.
Trends in Food Science and Technology
Elsevier., 85, 307-319.
https://doi.org/10.1016/j.tifs.2019.01.014
Lozano-Ojalvo D, Benedé S, Antunes CM, Bavaro SL, Bouchaud G, Costa A, Denery-Papini S, Díaz-Perales A, Garrido-Arandia M, Gavrović-Jankulović M, Hayen S, Martínez-Blanco M, Molina E, Monaci L, Pieters R, Villemin C, Wichers HJ, Wróblewska B, Willemsen LE, Roggen EL, van Bilsen JH. Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies. Trends in Food Science and Technology. 2019;85:307-319
Lozano-Ojalvo Daniel, Benedé Sara, Antunes Celia M., Bavaro Simona L., Bouchaud Grégory, Costa Ana, Denery-Papini Sandra, Díaz-Perales Araceli, Garrido-Arandia María, Gavrović-Jankulović Marija, Hayen Simone, Martínez-Blanco Mónica, Molina Elena, Monaci Linda, Pieters Raymond, Villemin Clelia, Wichers Harry J., Wróblewska Barbara, Willemsen Linette E.M., Roggen Erwin L., van Bilsen Jolanda H.M., "Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies" Trends in Food Science and Technology, 85 (2019):307-319,
https://doi.org/10.1016/j.tifs.2019.01.014 .
4
2
3

Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy

Mrkić, Ivan; Minić, Rajna; Popović, Dragan; Živković, Irena; Gavrović-Jankulović, Marija

(Elsevier, 2018)

TY  - JOUR
AU  - Mrkić, Ivan
AU  - Minić, Rajna
AU  - Popović, Dragan
AU  - Živković, Irena
AU  - Gavrović-Jankulović, Marija
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2887
AB  - Aim To investigate the immunomodulatory potential of a chimera composed of the receptor-binding domain of hemagglutinin 1 (H1s) from Influenza virus and Der p 2 (D2) allergen for allergen-specific immunotherapy of house-dust mite allergy (HDM). Main methods: H1sD2 chimera and D2 allergen were produced by genetic engineering in E. coli. Recombinant antigens were extracted from inclusion bodies by urea, then refolded and purified by immobilized-metal affinity chromatography (IMAC). Purity was verified by 2D-PAGE and secondary structures were assessed by CD spectroscopy. IgE reactivity of H1sD2 and D2 was tested in western blot with sera from 8 persons with clinical history of HDM allergy. Immunogenicity of H1sD2 and D2 were analyzed in Balb/c mice. Cytokine profile was analyzed by ELISA after stimulation of mouse spleen cells with H1sD2 and D2. Leukocyte population abundance of cells isolated from spleen and lymph node was assessed by flow cytometry. Key findings: Purified recombinant proteins H1sD2 (42 kDa) and D2 (15 kDa) revealed well defined secondary structures, and preserved IgE reactive epitopes. Analysis of supernatants of mouse spleen cells after stimulation with H1sD2 and D2, revealed a qualitatively different cytokine profile from H1sD2 immunized mouse cells (increase in IL10). CD8+ cells were decreased in the lymph node of D2 immunized mice, whereas H1sD2 immunization led to an increase of CD8+ cells in both the lymph node and the spleen. Significance: H1sD2 chimera attenuates Der p 2-inherent Th2 response and directs the immune response toward Th1 and Treg phenotype.
PB  - Elsevier
T2  - Life Sciences
T1  - Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy
VL  - 213
SP  - 158
EP  - 165
DO  - 10.1016/j.lfs.2018.10.036
ER  - 
@article{
author = "Mrkić, Ivan and Minić, Rajna and Popović, Dragan and Živković, Irena and Gavrović-Jankulović, Marija",
year = "2018",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2887",
abstract = "Aim To investigate the immunomodulatory potential of a chimera composed of the receptor-binding domain of hemagglutinin 1 (H1s) from Influenza virus and Der p 2 (D2) allergen for allergen-specific immunotherapy of house-dust mite allergy (HDM). Main methods: H1sD2 chimera and D2 allergen were produced by genetic engineering in E. coli. Recombinant antigens were extracted from inclusion bodies by urea, then refolded and purified by immobilized-metal affinity chromatography (IMAC). Purity was verified by 2D-PAGE and secondary structures were assessed by CD spectroscopy. IgE reactivity of H1sD2 and D2 was tested in western blot with sera from 8 persons with clinical history of HDM allergy. Immunogenicity of H1sD2 and D2 were analyzed in Balb/c mice. Cytokine profile was analyzed by ELISA after stimulation of mouse spleen cells with H1sD2 and D2. Leukocyte population abundance of cells isolated from spleen and lymph node was assessed by flow cytometry. Key findings: Purified recombinant proteins H1sD2 (42 kDa) and D2 (15 kDa) revealed well defined secondary structures, and preserved IgE reactive epitopes. Analysis of supernatants of mouse spleen cells after stimulation with H1sD2 and D2, revealed a qualitatively different cytokine profile from H1sD2 immunized mouse cells (increase in IL10). CD8+ cells were decreased in the lymph node of D2 immunized mice, whereas H1sD2 immunization led to an increase of CD8+ cells in both the lymph node and the spleen. Significance: H1sD2 chimera attenuates Der p 2-inherent Th2 response and directs the immune response toward Th1 and Treg phenotype.",
publisher = "Elsevier",
journal = "Life Sciences",
title = "Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy",
volume = "213",
pages = "158-165",
doi = "10.1016/j.lfs.2018.10.036"
}
Mrkić, I., Minić, R., Popović, D., Živković, I.,& Gavrović-Jankulović, M. (2018). Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy.
Life Sciences
Elsevier., 213, 158-165.
https://doi.org/10.1016/j.lfs.2018.10.036
Mrkić I, Minić R, Popović D, Živković I, Gavrović-Jankulović M. Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy. Life Sciences. 2018;213:158-165
Mrkić Ivan, Minić Rajna, Popović Dragan, Živković Irena, Gavrović-Jankulović Marija, "Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy" Life Sciences, 213 (2018):158-165,
https://doi.org/10.1016/j.lfs.2018.10.036 .
2
2
2

Supplementary material for the article: Nikolić, J.; Nešić, A.; Kull, S.; Schocker, F.; Jappe, U.; Gavrović-Jankulović, M. Employment of Proteomic and Immunological Based Methods for the Identification of Catalase as Novel Allergen from Banana. Journal of Proteomics 2018, 175, 87–94. https://doi.org/10.1016/j.jprot.2018.01.007

Nikolić, Jasna; Nešić, Andrijana N.; Kull, Skadi; Schocker, Frauke; Jappe, Uta; Gavrović-Jankulović, Marija

(Elsevier Science Bv, Amsterdam, 2018)

TY  - BOOK
AU  - Nikolić, Jasna
AU  - Nešić, Andrijana N.
AU  - Kull, Skadi
AU  - Schocker, Frauke
AU  - Jappe, Uta
AU  - Gavrović-Jankulović, Marija
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3203
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Proteomics
T1  - Supplementary material for the article: Nikolić, J.; Nešić, A.; Kull, S.; Schocker, F.; Jappe, U.; Gavrović-Jankulović, M.  Employment of Proteomic and Immunological Based Methods for the Identification of  Catalase as Novel Allergen from Banana. Journal of Proteomics 2018, 175, 87–94.  https://doi.org/10.1016/j.jprot.2018.01.007
ER  - 
@book{
author = "Nikolić, Jasna and Nešić, Andrijana N. and Kull, Skadi and Schocker, Frauke and Jappe, Uta and Gavrović-Jankulović, Marija",
year = "2018",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3203",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Proteomics",
title = "Supplementary material for the article: Nikolić, J.; Nešić, A.; Kull, S.; Schocker, F.; Jappe, U.; Gavrović-Jankulović, M.  Employment of Proteomic and Immunological Based Methods for the Identification of  Catalase as Novel Allergen from Banana. Journal of Proteomics 2018, 175, 87–94.  https://doi.org/10.1016/j.jprot.2018.01.007"
}
Nikolić, J., Nešić, A. N., Kull, S., Schocker, F., Jappe, U.,& Gavrović-Jankulović, M. (2018). Supplementary material for the article: Nikolić, J.; Nešić, A.; Kull, S.; Schocker, F.; Jappe, U.; Gavrović-Jankulović, M.  Employment of Proteomic and Immunological Based Methods for the Identification of  Catalase as Novel Allergen from Banana. Journal of Proteomics 2018, 175, 87–94.  https://doi.org/10.1016/j.jprot.2018.01.007.
Journal of Proteomics
Elsevier Science Bv, Amsterdam..
Nikolić J, Nešić AN, Kull S, Schocker F, Jappe U, Gavrović-Jankulović M. Supplementary material for the article: Nikolić, J.; Nešić, A.; Kull, S.; Schocker, F.; Jappe, U.; Gavrović-Jankulović, M.  Employment of Proteomic and Immunological Based Methods for the Identification of  Catalase as Novel Allergen from Banana. Journal of Proteomics 2018, 175, 87–94.  https://doi.org/10.1016/j.jprot.2018.01.007. Journal of Proteomics. 2018;
Nikolić Jasna, Nešić Andrijana N., Kull Skadi, Schocker Frauke, Jappe Uta, Gavrović-Jankulović Marija, "Supplementary material for the article: Nikolić, J.; Nešić, A.; Kull, S.; Schocker, F.; Jappe, U.; Gavrović-Jankulović, M.  Employment of Proteomic and Immunological Based Methods for the Identification of  Catalase as Novel Allergen from Banana. Journal of Proteomics 2018, 175, 87–94.  https://doi.org/10.1016/j.jprot.2018.01.007" Journal of Proteomics (2018)

Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana

Nikolić, Jasna; Nešić, Andrijana N.; Kull, Skadi; Schocker, Frauke; Jappe, Uta; Gavrović-Jankulović, Marija

(Elsevier Science Bv, Amsterdam, 2018)

TY  - JOUR
AU  - Nikolić, Jasna
AU  - Nešić, Andrijana N.
AU  - Kull, Skadi
AU  - Schocker, Frauke
AU  - Jappe, Uta
AU  - Gavrović-Jankulović, Marija
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2117
AB  - Diagnostic reagents based on food allergen extracts often lack sufficient sensitivity. The introduction of well characterized food allergens in molecular allergy diagnosis has been recognized as valid approach to circumvent unstandardized allergen extracts. Banana fruit (Musa acuminate) is a well-established allergen source which besides six characterized allergens, contains unidentified IgE reactive proteins whose clinical relevance remains undefined. By employment of a combinatorial peptide ligand library (CPLL) methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis, a novel allergen from banana fruit was detected in banana as catalase. A recombinant homologue of natural catalase was produced, isolated and biochemically characterized. The recombinant protein showed IgE reactivity in 7 out of 13 tested patients with suspected allergy to banana in immunoblot. Novel banana fruit allergens should be added as components to allergen-microarrays for the diagnosis and the monitoring of banana allergy. Significance: By employment of CPLL methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis catalase from banana fruit is identified as a novel allergen, with proposed designation as Mus a 7. IgE reactive recombinant Mus a 7 was produced and should be included in a component-resolved allergy diagnosis.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Proteomics
T1  - Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana
VL  - 175
SP  - 87
EP  - 94
DO  - 10.1016/j.jprot.2018.01.007
ER  - 
@article{
author = "Nikolić, Jasna and Nešić, Andrijana N. and Kull, Skadi and Schocker, Frauke and Jappe, Uta and Gavrović-Jankulović, Marija",
year = "2018",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2117",
abstract = "Diagnostic reagents based on food allergen extracts often lack sufficient sensitivity. The introduction of well characterized food allergens in molecular allergy diagnosis has been recognized as valid approach to circumvent unstandardized allergen extracts. Banana fruit (Musa acuminate) is a well-established allergen source which besides six characterized allergens, contains unidentified IgE reactive proteins whose clinical relevance remains undefined. By employment of a combinatorial peptide ligand library (CPLL) methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis, a novel allergen from banana fruit was detected in banana as catalase. A recombinant homologue of natural catalase was produced, isolated and biochemically characterized. The recombinant protein showed IgE reactivity in 7 out of 13 tested patients with suspected allergy to banana in immunoblot. Novel banana fruit allergens should be added as components to allergen-microarrays for the diagnosis and the monitoring of banana allergy. Significance: By employment of CPLL methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis catalase from banana fruit is identified as a novel allergen, with proposed designation as Mus a 7. IgE reactive recombinant Mus a 7 was produced and should be included in a component-resolved allergy diagnosis.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Proteomics",
title = "Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana",
volume = "175",
pages = "87-94",
doi = "10.1016/j.jprot.2018.01.007"
}
Nikolić, J., Nešić, A. N., Kull, S., Schocker, F., Jappe, U.,& Gavrović-Jankulović, M. (2018). Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana.
Journal of Proteomics
Elsevier Science Bv, Amsterdam., 175, 87-94.
https://doi.org/10.1016/j.jprot.2018.01.007
Nikolić J, Nešić AN, Kull S, Schocker F, Jappe U, Gavrović-Jankulović M. Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana. Journal of Proteomics. 2018;175:87-94
Nikolić Jasna, Nešić Andrijana N., Kull Skadi, Schocker Frauke, Jappe Uta, Gavrović-Jankulović Marija, "Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana" Journal of Proteomics, 175 (2018):87-94,
https://doi.org/10.1016/j.jprot.2018.01.007 .
1
7
6
6

Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy

Mrkić, Ivan; Minić, Rajna; Popović, Dragan; Živković, Irena; Gavrović-Jankulović, Marija

(Pergamon-Elsevier Science Ltd, Oxford, 2018)

TY  - JOUR
AU  - Mrkić, Ivan
AU  - Minić, Rajna
AU  - Popović, Dragan
AU  - Živković, Irena
AU  - Gavrović-Jankulović, Marija
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2236
AB  - Aim To investigate the immunomodulatory potential of a chimera composed of the receptor-binding domain of hemagglutinin 1 (H1s) from Influenza virus and Der p 2 (D2) allergen for allergen-specific immunotherapy of house-dust mite allergy (HDM). Main methods: H1sD2 chimera and D2 allergen were produced by genetic engineering in E. coli. Recombinant antigens were extracted from inclusion bodies by urea, then refolded and purified by immobilized-metal affinity chromatography (IMAC). Purity was verified by 2D-PAGE and secondary structures were assessed by CD spectroscopy. IgE reactivity of H1sD2 and D2 was tested in western blot with sera from 8 persons with clinical history of HDM allergy. Immunogenicity of H1sD2 and D2 were analyzed in Balb/c mice. Cytokine profile was analyzed by ELISA after stimulation of mouse spleen cells with H1sD2 and D2. Leukocyte population abundance of cells isolated from spleen and lymph node was assessed by flow cytometry. Key findings: Purified recombinant proteins H1sD2 (42 kDa) and D2 (15 kDa) revealed well defined secondary structures, and preserved IgE reactive epitopes. Analysis of supernatants of mouse spleen cells after stimulation with H1sD2 and D2, revealed a qualitatively different cytokine profile from H1sD2 immunized mouse cells (increase in IL10). CD8+ cells were decreased in the lymph node of D2 immunized mice, whereas H1sD2 immunization led to an increase of CD8+ cells in both the lymph node and the spleen. Significance: H1sD2 chimera attenuates Der p 2-inherent Th2 response and directs the immune response toward Th1 and Treg phenotype.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Life Sciences
T1  - Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy
VL  - 213
SP  - 158
EP  - 165
DO  - 10.1016/j.lfs.2018.10.036
ER  - 
@article{
author = "Mrkić, Ivan and Minić, Rajna and Popović, Dragan and Živković, Irena and Gavrović-Jankulović, Marija",
year = "2018",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2236",
abstract = "Aim To investigate the immunomodulatory potential of a chimera composed of the receptor-binding domain of hemagglutinin 1 (H1s) from Influenza virus and Der p 2 (D2) allergen for allergen-specific immunotherapy of house-dust mite allergy (HDM). Main methods: H1sD2 chimera and D2 allergen were produced by genetic engineering in E. coli. Recombinant antigens were extracted from inclusion bodies by urea, then refolded and purified by immobilized-metal affinity chromatography (IMAC). Purity was verified by 2D-PAGE and secondary structures were assessed by CD spectroscopy. IgE reactivity of H1sD2 and D2 was tested in western blot with sera from 8 persons with clinical history of HDM allergy. Immunogenicity of H1sD2 and D2 were analyzed in Balb/c mice. Cytokine profile was analyzed by ELISA after stimulation of mouse spleen cells with H1sD2 and D2. Leukocyte population abundance of cells isolated from spleen and lymph node was assessed by flow cytometry. Key findings: Purified recombinant proteins H1sD2 (42 kDa) and D2 (15 kDa) revealed well defined secondary structures, and preserved IgE reactive epitopes. Analysis of supernatants of mouse spleen cells after stimulation with H1sD2 and D2, revealed a qualitatively different cytokine profile from H1sD2 immunized mouse cells (increase in IL10). CD8+ cells were decreased in the lymph node of D2 immunized mice, whereas H1sD2 immunization led to an increase of CD8+ cells in both the lymph node and the spleen. Significance: H1sD2 chimera attenuates Der p 2-inherent Th2 response and directs the immune response toward Th1 and Treg phenotype.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Life Sciences",
title = "Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy",
volume = "213",
pages = "158-165",
doi = "10.1016/j.lfs.2018.10.036"
}
Mrkić, I., Minić, R., Popović, D., Živković, I.,& Gavrović-Jankulović, M. (2018). Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy.
Life Sciences
Pergamon-Elsevier Science Ltd, Oxford., 213, 158-165.
https://doi.org/10.1016/j.lfs.2018.10.036
Mrkić I, Minić R, Popović D, Živković I, Gavrović-Jankulović M. Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy. Life Sciences. 2018;213:158-165
Mrkić Ivan, Minić Rajna, Popović Dragan, Živković Irena, Gavrović-Jankulović Marija, "Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy" Life Sciences, 213 (2018):158-165,
https://doi.org/10.1016/j.lfs.2018.10.036 .
2
2
2

Evaluation of Food Allergy in Children by Skin Prick Tests with Commercial Extracts and Fresh Foods, Specific IgE and, Open Oral Food Challenge: Our Five Years Experience in Food Allergy Work-up

Zivanovic, Mirjana; Atanasković-Marković, Marina; Međo, Biljana; Gavrović-Jankulović, Marija; Smiljanić, Katarina; Tmušić, Vladimir; Đurić, Vojislav

(Iranian Scientific Society Medical Entomology, Tehran, 2017)

TY  - JOUR
AU  - Zivanovic, Mirjana
AU  - Atanasković-Marković, Marina
AU  - Međo, Biljana
AU  - Gavrović-Jankulović, Marija
AU  - Smiljanić, Katarina
AU  - Tmušić, Vladimir
AU  - Đurić, Vojislav
PY  - 2017
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2469
AB  - IgE-mediated food allergy affects 6-8% of children. Our study aimed to define the correlations between the results obtained with skin prick tests (SPTs) using commercial extracts and fresh foods, and the correlations between these result and those obtained with specific IgE (sIgE) and/or challenge. Children aged from 2 months to 6 years were recruited prospectively. Overall 571 children were positive to one food. In all children we performed SPT using commercial extracts of suspected food and fresh foods and sIgE. If SPT and sIgE test results did not correspond to the history, we performed open oral food challenge. Sensitivity of SPT with commercial extracts for all tested food was poor (3-35%), while sensitivity of fresh food skin prick tests (FFSPT) was excellent (50-100%), and showed correlation with open oral food challenge (p lt 0.001). Our results suggest that fresh food extracts are more effective in detecting sensitization and with levels of sIgE greater than class 3 could predict clinical reactivity, without the need for potentially hazardous food challenges.
PB  - Iranian Scientific Society Medical Entomology, Tehran
T2  - Iranian Journal of Allergy Asthma and Immunology
T1  - Evaluation of Food Allergy in Children by Skin Prick Tests with Commercial Extracts and Fresh Foods, Specific IgE and, Open Oral Food Challenge: Our Five Years Experience in Food Allergy Work-up
VL  - 16
IS  - 2
SP  - 127
EP  - 132
ER  - 
@article{
author = "Zivanovic, Mirjana and Atanasković-Marković, Marina and Međo, Biljana and Gavrović-Jankulović, Marija and Smiljanić, Katarina and Tmušić, Vladimir and Đurić, Vojislav",
year = "2017",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2469",
abstract = "IgE-mediated food allergy affects 6-8% of children. Our study aimed to define the correlations between the results obtained with skin prick tests (SPTs) using commercial extracts and fresh foods, and the correlations between these result and those obtained with specific IgE (sIgE) and/or challenge. Children aged from 2 months to 6 years were recruited prospectively. Overall 571 children were positive to one food. In all children we performed SPT using commercial extracts of suspected food and fresh foods and sIgE. If SPT and sIgE test results did not correspond to the history, we performed open oral food challenge. Sensitivity of SPT with commercial extracts for all tested food was poor (3-35%), while sensitivity of fresh food skin prick tests (FFSPT) was excellent (50-100%), and showed correlation with open oral food challenge (p lt 0.001). Our results suggest that fresh food extracts are more effective in detecting sensitization and with levels of sIgE greater than class 3 could predict clinical reactivity, without the need for potentially hazardous food challenges.",
publisher = "Iranian Scientific Society Medical Entomology, Tehran",
journal = "Iranian Journal of Allergy Asthma and Immunology",
title = "Evaluation of Food Allergy in Children by Skin Prick Tests with Commercial Extracts and Fresh Foods, Specific IgE and, Open Oral Food Challenge: Our Five Years Experience in Food Allergy Work-up",
volume = "16",
number = "2",
pages = "127-132"
}
Zivanovic, M., Atanasković-Marković, M., Međo, B., Gavrović-Jankulović, M., Smiljanić, K., Tmušić, V.,& Đurić, V. (2017). Evaluation of Food Allergy in Children by Skin Prick Tests with Commercial Extracts and Fresh Foods, Specific IgE and, Open Oral Food Challenge: Our Five Years Experience in Food Allergy Work-up.
Iranian Journal of Allergy Asthma and Immunology
Iranian Scientific Society Medical Entomology, Tehran., 16(2), 127-132.
Zivanovic M, Atanasković-Marković M, Međo B, Gavrović-Jankulović M, Smiljanić K, Tmušić V, Đurić V. Evaluation of Food Allergy in Children by Skin Prick Tests with Commercial Extracts and Fresh Foods, Specific IgE and, Open Oral Food Challenge: Our Five Years Experience in Food Allergy Work-up. Iranian Journal of Allergy Asthma and Immunology. 2017;16(2):127-132
Zivanovic Mirjana, Atanasković-Marković Marina, Međo Biljana, Gavrović-Jankulović Marija, Smiljanić Katarina, Tmušić Vladimir, Đurić Vojislav, "Evaluation of Food Allergy in Children by Skin Prick Tests with Commercial Extracts and Fresh Foods, Specific IgE and, Open Oral Food Challenge: Our Five Years Experience in Food Allergy Work-up" Iranian Journal of Allergy Asthma and Immunology, 16, no. 2 (2017):127-132
5
6

Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells

Nikolić, Jasna; Nešić, Andrijana N.; Čavić, Milena; Đorđević, Neda O.; Andelković, Uroš; Atanasković-Marković, Marina; Drakulić, Branko J.; Gavrović-Jankulović, Marija

(Elsevier Science Bv, Amsterdam, 2017)

TY  - JOUR
AU  - Nikolić, Jasna
AU  - Nešić, Andrijana N.
AU  - Čavić, Milena
AU  - Đorđević, Neda O.
AU  - Andelković, Uroš
AU  - Atanasković-Marković, Marina
AU  - Drakulić, Branko J.
AU  - Gavrović-Jankulović, Marija
PY  - 2017
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2384
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2954
AB  - ackground: Protein oxidation can occur as a consequence of lipid peroxidation during food processing. The aim of this work was to explore the effect of malondialdehyde (MDA) modification of ovalbumin (OVA) on its interaction with T84 intestinal cells. Methods: Molecular dynamics simulation was employed for the prediction of MDA modification in the OVA, while introduced structural changes were evaluated by measurement of carbonyl group content, fluorescence spectra, MS/MS analysis, and IgE reactivity. Effects of MDA modified OVA on T84 epithelial cells were analyzed by gene expression for pro-inflammatory cytokines and protein secretion. Results: Out of 9 predicted, five modified Lys residues were confirmed by MS/MS analysis: (51)TQINKVVR(58), (85)DILNQITKPNDVYSFSLASR(104), (111)YPILPEYLQCVKELYR(126), (187)AFKDEDTQAMPFR(99), (KIKVYLPR284)-K-277, and (IKVYLPR284)-I-278. The introduced MDA modifications influenced profile of IgE reactivity to OVA. Treatment of T84 epithelial cells with OVA and OVA modified with 1 mM MDA, induced up-regulation of pro-inflammatory cytokines (IL-1 beta,IL-25, IL-33, TSLP and TNF alpha), while OVA modification with 10 mM MDA induced down regulation of the cytokine expression profile, except for IL-1 beta. OVA and OVA modified with 1 mM MDA induced secretion of epithelial cells specific cytokine IL-33. Conclusions: This finding indicated that OVA and its MDA modified form have the potential to trigger the innate immunity by inducing up-regulation and secretion of pro-allergenic IL-33 in T84 intestinal epithelial cells. General significance: Interactions of ovalbumin and its MDA modified form with intestinal epithelial cells can induce a specific immunological priming necessary for the downstream activation of innate immunity. (C) 2016 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Biochimica et Biophysica Acta: General Subjects
T1  - Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells
VL  - 1861
IS  - 2
SP  - 126
EP  - 134
DO  - 10.1016/j.bbagen.2016.11.021
ER  - 
@article{
author = "Nikolić, Jasna and Nešić, Andrijana N. and Čavić, Milena and Đorđević, Neda O. and Andelković, Uroš and Atanasković-Marković, Marina and Drakulić, Branko J. and Gavrović-Jankulović, Marija",
year = "2017",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2384, http://cherry.chem.bg.ac.rs/handle/123456789/2954",
abstract = "ackground: Protein oxidation can occur as a consequence of lipid peroxidation during food processing. The aim of this work was to explore the effect of malondialdehyde (MDA) modification of ovalbumin (OVA) on its interaction with T84 intestinal cells. Methods: Molecular dynamics simulation was employed for the prediction of MDA modification in the OVA, while introduced structural changes were evaluated by measurement of carbonyl group content, fluorescence spectra, MS/MS analysis, and IgE reactivity. Effects of MDA modified OVA on T84 epithelial cells were analyzed by gene expression for pro-inflammatory cytokines and protein secretion. Results: Out of 9 predicted, five modified Lys residues were confirmed by MS/MS analysis: (51)TQINKVVR(58), (85)DILNQITKPNDVYSFSLASR(104), (111)YPILPEYLQCVKELYR(126), (187)AFKDEDTQAMPFR(99), (KIKVYLPR284)-K-277, and (IKVYLPR284)-I-278. The introduced MDA modifications influenced profile of IgE reactivity to OVA. Treatment of T84 epithelial cells with OVA and OVA modified with 1 mM MDA, induced up-regulation of pro-inflammatory cytokines (IL-1 beta,IL-25, IL-33, TSLP and TNF alpha), while OVA modification with 10 mM MDA induced down regulation of the cytokine expression profile, except for IL-1 beta. OVA and OVA modified with 1 mM MDA induced secretion of epithelial cells specific cytokine IL-33. Conclusions: This finding indicated that OVA and its MDA modified form have the potential to trigger the innate immunity by inducing up-regulation and secretion of pro-allergenic IL-33 in T84 intestinal epithelial cells. General significance: Interactions of ovalbumin and its MDA modified form with intestinal epithelial cells can induce a specific immunological priming necessary for the downstream activation of innate immunity. (C) 2016 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Biochimica et Biophysica Acta: General Subjects",
title = "Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells",
volume = "1861",
number = "2",
pages = "126-134",
doi = "10.1016/j.bbagen.2016.11.021"
}
Nikolić, J., Nešić, A. N., Čavić, M., Đorđević, N. O., Andelković, U., Atanasković-Marković, M., Drakulić, B. J.,& Gavrović-Jankulović, M. (2017). Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells.
Biochimica et Biophysica Acta: General Subjects
Elsevier Science Bv, Amsterdam., 1861(2), 126-134.
https://doi.org/10.1016/j.bbagen.2016.11.021
Nikolić J, Nešić AN, Čavić M, Đorđević NO, Andelković U, Atanasković-Marković M, Drakulić BJ, Gavrović-Jankulović M. Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells. Biochimica et Biophysica Acta: General Subjects. 2017;1861(2):126-134
Nikolić Jasna, Nešić Andrijana N., Čavić Milena, Đorđević Neda O., Andelković Uroš, Atanasković-Marković Marina, Drakulić Branko J., Gavrović-Jankulović Marija, "Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells" Biochimica et Biophysica Acta: General Subjects, 1861, no. 2 (2017):126-134,
https://doi.org/10.1016/j.bbagen.2016.11.021 .
5
4
5

Omics methods as a tool for investigation of food allergies

Anđelković, Uroš; Gavrović-Jankulović, Marija; Martinovic, Tamara; Josic, Djuro

(Elsevier Sci Ltd, Oxford, 2017)

TY  - JOUR
AU  - Anđelković, Uroš
AU  - Gavrović-Jankulović, Marija
AU  - Martinovic, Tamara
AU  - Josic, Djuro
PY  - 2017
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2963
AB  - Use of foodomics, mostly proteomic and genomic based methods, for study of allergens in food is presented. Immunological methods and nucleic acid-based methods are still most frequently used for diagnosis of allergies and for qualitative and quantitative determination of food allergens. They are sensitive, and can be used for the determination of allergens in trace concentrations. However, lack of specificity and cross-reaction of some antibodies can still be a relevant source of bias. The epitopes of protein allergens with posttranslational modifications and their changes originated during food processing cannot be traced by use of nucleic acid-based strategies. Recent developments of both antibody and nucleic acid-based biosensors, their miniaturization and increasing application of nanotechnology, significantly supported further use of both strategies. Regarding accuracy, reliability and sensitivity, mass spectrometry-based methods bring important advantage over both above presented strategies. Furthermore, the increasing use of mass spectrometry (MS) is discussed. Combined with proper sample preparation, liquid chromatography (LC) and/or different electrophoretic methods, targeted approach in mass spectrometry-based allergen analysis brings an additional strategic advance. However, MS is still rarely used for high-throughput analyses and detection and quantification of allergens for the reasons of price and relatively long time necessary for analysis. Recent developments of new high-resolution instruments are encouraging and enable development in the direction of a high-throughput strategy. Consequently, fast, very sensitive, reliable and accurate detection and quantification of allergens in highly complex samples such as food matrices, and the use of MS in routine determination of allergens can be reached in near future.
PB  - Elsevier Sci Ltd, Oxford
T2  - Trends in Analytical Chemistry / TRAC
T1  - Omics methods as a tool for investigation of food allergies
VL  - 96
SP  - 107
EP  - 115
DO  - 10.1016/j.trac.2017.07.011
ER  - 
@article{
author = "Anđelković, Uroš and Gavrović-Jankulović, Marija and Martinovic, Tamara and Josic, Djuro",
year = "2017",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2963",
abstract = "Use of foodomics, mostly proteomic and genomic based methods, for study of allergens in food is presented. Immunological methods and nucleic acid-based methods are still most frequently used for diagnosis of allergies and for qualitative and quantitative determination of food allergens. They are sensitive, and can be used for the determination of allergens in trace concentrations. However, lack of specificity and cross-reaction of some antibodies can still be a relevant source of bias. The epitopes of protein allergens with posttranslational modifications and their changes originated during food processing cannot be traced by use of nucleic acid-based strategies. Recent developments of both antibody and nucleic acid-based biosensors, their miniaturization and increasing application of nanotechnology, significantly supported further use of both strategies. Regarding accuracy, reliability and sensitivity, mass spectrometry-based methods bring important advantage over both above presented strategies. Furthermore, the increasing use of mass spectrometry (MS) is discussed. Combined with proper sample preparation, liquid chromatography (LC) and/or different electrophoretic methods, targeted approach in mass spectrometry-based allergen analysis brings an additional strategic advance. However, MS is still rarely used for high-throughput analyses and detection and quantification of allergens for the reasons of price and relatively long time necessary for analysis. Recent developments of new high-resolution instruments are encouraging and enable development in the direction of a high-throughput strategy. Consequently, fast, very sensitive, reliable and accurate detection and quantification of allergens in highly complex samples such as food matrices, and the use of MS in routine determination of allergens can be reached in near future.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Trends in Analytical Chemistry / TRAC",
title = "Omics methods as a tool for investigation of food allergies",
volume = "96",
pages = "107-115",
doi = "10.1016/j.trac.2017.07.011"
}
Anđelković, U., Gavrović-Jankulović, M., Martinovic, T.,& Josic, D. (2017). Omics methods as a tool for investigation of food allergies.
Trends in Analytical Chemistry / TRAC
Elsevier Sci Ltd, Oxford., 96, 107-115.
https://doi.org/10.1016/j.trac.2017.07.011
Anđelković U, Gavrović-Jankulović M, Martinovic T, Josic D. Omics methods as a tool for investigation of food allergies. Trends in Analytical Chemistry / TRAC. 2017;96:107-115
Anđelković Uroš, Gavrović-Jankulović Marija, Martinovic Tamara, Josic Djuro, "Omics methods as a tool for investigation of food allergies" Trends in Analytical Chemistry / TRAC, 96 (2017):107-115,
https://doi.org/10.1016/j.trac.2017.07.011 .
3
17
11
14

Supplementary data for article: Marinkovic, E.; Djokic, R.; Lukic, I.; Filipovic, A.; Inic-Kanada, A.; Kosanovic, D.; Gavrovic-Jankulovic, M.; Stojanovic, M. Modulation of Functional Characteristics of Resident and Thioglycollate-Elicited Peritoneal Murine Macrophages by a Recombinant Banana Lectin. PLoS ONE 2017, 12 (2). https://doi.org/10.1371/journal.pone.017246

Marinković, Emilija; Djokić, Radmila; Lukić, Ivana; Filipović, Ana; Inić-Kanada, Aleksandra; Kosanović, Dejana; Gavrović-Jankulović, Marija; Stojanović, Marijana M.

(Public Library Science, San Francisco, 2017)

TY  - BOOK
AU  - Marinković, Emilija
AU  - Djokić, Radmila
AU  - Lukić, Ivana
AU  - Filipović, Ana
AU  - Inić-Kanada, Aleksandra
AU  - Kosanović, Dejana
AU  - Gavrović-Jankulović, Marija
AU  - Stojanović, Marijana M.
PY  - 2017
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3037
PB  - Public Library Science, San Francisco
T2  - PLoS ONE
T1  - Supplementary data for article: Marinkovic, E.; Djokic, R.; Lukic, I.; Filipovic, A.; Inic-Kanada, A.; Kosanovic, D.; Gavrovic-Jankulovic, M.; Stojanovic, M. Modulation of Functional Characteristics of Resident and Thioglycollate-Elicited Peritoneal Murine Macrophages by a Recombinant Banana Lectin. PLoS ONE 2017, 12 (2). https://doi.org/10.1371/journal.pone.017246
ER  - 
@book{
author = "Marinković, Emilija and Djokić, Radmila and Lukić, Ivana and Filipović, Ana and Inić-Kanada, Aleksandra and Kosanović, Dejana and Gavrović-Jankulović, Marija and Stojanović, Marijana M.",
year = "2017",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3037",
publisher = "Public Library Science, San Francisco",
journal = "PLoS ONE",
title = "Supplementary data for article: Marinkovic, E.; Djokic, R.; Lukic, I.; Filipovic, A.; Inic-Kanada, A.; Kosanovic, D.; Gavrovic-Jankulovic, M.; Stojanovic, M. Modulation of Functional Characteristics of Resident and Thioglycollate-Elicited Peritoneal Murine Macrophages by a Recombinant Banana Lectin. PLoS ONE 2017, 12 (2). https://doi.org/10.1371/journal.pone.017246"
}
Marinković, E., Djokić, R., Lukić, I., Filipović, A., Inić-Kanada, A., Kosanović, D., Gavrović-Jankulović, M.,& Stojanović, M. M. (2017). Supplementary data for article: Marinkovic, E.; Djokic, R.; Lukic, I.; Filipovic, A.; Inic-Kanada, A.; Kosanovic, D.; Gavrovic-Jankulovic, M.; Stojanovic, M. Modulation of Functional Characteristics of Resident and Thioglycollate-Elicited Peritoneal Murine Macrophages by a Recombinant Banana Lectin. PLoS ONE 2017, 12 (2). https://doi.org/10.1371/journal.pone.017246.
PLoS ONE
Public Library Science, San Francisco..
Marinković E, Djokić R, Lukić I, Filipović A, Inić-Kanada A, Kosanović D, Gavrović-Jankulović M, Stojanović MM. Supplementary data for article: Marinkovic, E.; Djokic, R.; Lukic, I.; Filipovic, A.; Inic-Kanada, A.; Kosanovic, D.; Gavrovic-Jankulovic, M.; Stojanovic, M. Modulation of Functional Characteristics of Resident and Thioglycollate-Elicited Peritoneal Murine Macrophages by a Recombinant Banana Lectin. PLoS ONE 2017, 12 (2). https://doi.org/10.1371/journal.pone.017246. PLoS ONE. 2017;
Marinković Emilija, Djokić Radmila, Lukić Ivana, Filipović Ana, Inić-Kanada Aleksandra, Kosanović Dejana, Gavrović-Jankulović Marija, Stojanović Marijana M., "Supplementary data for article: Marinkovic, E.; Djokic, R.; Lukic, I.; Filipovic, A.; Inic-Kanada, A.; Kosanovic, D.; Gavrovic-Jankulovic, M.; Stojanovic, M. Modulation of Functional Characteristics of Resident and Thioglycollate-Elicited Peritoneal Murine Macrophages by a Recombinant Banana Lectin. PLoS ONE 2017, 12 (2). https://doi.org/10.1371/journal.pone.017246" PLoS ONE (2017)

Synthesis of medium-chain length capsinoids from coconut oil catalyzed by Candida rugosa lipases

Trbojević-Ivić, Jovana; Milosavić, Nenad; Dimitrijević, Aleksandra; Gavrović-Jankulović, Marija; Bezbradica, Dejan; Kolarski, Dušan; Veličković, Dušan

(Elsevier Sci Ltd, Oxford, 2017)

TY  - JOUR
AU  - Trbojević-Ivić, Jovana
AU  - Milosavić, Nenad
AU  - Dimitrijević, Aleksandra
AU  - Gavrović-Jankulović, Marija
AU  - Bezbradica, Dejan
AU  - Kolarski, Dušan
AU  - Veličković, Dušan
PY  - 2017
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3102
AB  - A commercial preparation of Candida rugosa lipases (CRL) was tested for the production of capsinoids by esterification of vanillyl alcohol (VA) with free fatty acids (FA) and coconut oil (CO) as acyl donors. Screening of FA chain length indicated that C8-C12 FA (the most common FA found in CO triglycerides) are the best acyl-donors, yielding 80-85% of their specific capsinoids. Hence, when CO, which is rich in these FA, was used as the substrate, a mixture of capsinoids (vanillyl caprylate, vanillyl decanoate and vanillyl laurate) was obtained. The findings presented here suggest that our experimental method can be applied for the enrichment of CO with capsinoids, thus giving it additional health promoting properties.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Synthesis of medium-chain length capsinoids from coconut oil catalyzed by Candida rugosa lipases
VL  - 218
SP  - 505
EP  - 508
DO  - 10.1016/j.foodchem.2016.09.049
ER  - 
@article{
author = "Trbojević-Ivić, Jovana and Milosavić, Nenad and Dimitrijević, Aleksandra and Gavrović-Jankulović, Marija and Bezbradica, Dejan and Kolarski, Dušan and Veličković, Dušan",
year = "2017",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3102",
abstract = "A commercial preparation of Candida rugosa lipases (CRL) was tested for the production of capsinoids by esterification of vanillyl alcohol (VA) with free fatty acids (FA) and coconut oil (CO) as acyl donors. Screening of FA chain length indicated that C8-C12 FA (the most common FA found in CO triglycerides) are the best acyl-donors, yielding 80-85% of their specific capsinoids. Hence, when CO, which is rich in these FA, was used as the substrate, a mixture of capsinoids (vanillyl caprylate, vanillyl decanoate and vanillyl laurate) was obtained. The findings presented here suggest that our experimental method can be applied for the enrichment of CO with capsinoids, thus giving it additional health promoting properties.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Synthesis of medium-chain length capsinoids from coconut oil catalyzed by Candida rugosa lipases",
volume = "218",
pages = "505-508",
doi = "10.1016/j.foodchem.2016.09.049"
}
Trbojević-Ivić, J., Milosavić, N., Dimitrijević, A., Gavrović-Jankulović, M., Bezbradica, D., Kolarski, D.,& Veličković, D. (2017). Synthesis of medium-chain length capsinoids from coconut oil catalyzed by Candida rugosa lipases.
Food Chemistry
Elsevier Sci Ltd, Oxford., 218, 505-508.
https://doi.org/10.1016/j.foodchem.2016.09.049
Trbojević-Ivić J, Milosavić N, Dimitrijević A, Gavrović-Jankulović M, Bezbradica D, Kolarski D, Veličković D. Synthesis of medium-chain length capsinoids from coconut oil catalyzed by Candida rugosa lipases. Food Chemistry. 2017;218:505-508
Trbojević-Ivić Jovana, Milosavić Nenad, Dimitrijević Aleksandra, Gavrović-Jankulović Marija, Bezbradica Dejan, Kolarski Dušan, Veličković Dušan, "Synthesis of medium-chain length capsinoids from coconut oil catalyzed by Candida rugosa lipases" Food Chemistry, 218 (2017):505-508,
https://doi.org/10.1016/j.foodchem.2016.09.049 .
12
8
9

Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells

Nikolić, Jasna; Nešić, Andrijana N.; Čavić, Milena; Đorđević, Neda O.; Andelković, Uroš; Atanasković-Marković, Marina; Drakulić, Branko J.; Gavrović-Jankulović, Marija

(Elsevier Science Bv, Amsterdam, 2017)

TY  - JOUR
AU  - Nikolić, Jasna
AU  - Nešić, Andrijana N.
AU  - Čavić, Milena
AU  - Đorđević, Neda O.
AU  - Andelković, Uroš
AU  - Atanasković-Marković, Marina
AU  - Drakulić, Branko J.
AU  - Gavrović-Jankulović, Marija
PY  - 2017
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2384
AB  - Background: Protein oxidation can occur as a consequence of lipid peroxidation during food processing. The aim of this work was to explore the effect of malondialdehyde (MDA) modification of ovalbumin (OVA) on its interaction with T84 intestinal cells. Methods: Molecular dynamics simulation was employed for the prediction of MDA modification in the OVA, while introduced structural changes were evaluated by measurement of carbonyl group content, fluorescence spectra, MS/MS analysis, and IgE reactivity. Effects of MDA modified OVA on T84 epithelial cells were analyzed by gene expression for pro-inflammatory cytokines and protein secretion. Results: Out of 9 predicted, five modified Lys residues were confirmed by MS/MS analysis: (51)TQINKVVR(58), (85)DILNQITKPNDVYSFSLASR(104), (111)YPILPEYLQCVKELYR(126), (187)AFKDEDTQAMPFR(99), (KIKVYLPR284)-K-277, and (IKVYLPR284)-I-278. The introduced MDA modifications influenced profile of IgE reactivity to OVA. Treatment of T84 epithelial cells with OVA and OVA modified with 1 mM MDA, induced up-regulation of pro-inflammatory cytokines (IL-1 beta,IL-25, IL-33, TSLP and TNF alpha), while OVA modification with 10 mM MDA induced down regulation of the cytokine expression profile, except for IL-1 beta. OVA and OVA modified with 1 mM MDA induced secretion of epithelial cells specific cytokine IL-33. Conclusions: This finding indicated that OVA and its MDA modified form have the potential to trigger the innate immunity by inducing up-regulation and secretion of pro-allergenic IL-33 in T84 intestinal epithelial cells. General significance: Interactions of ovalbumin and its MDA modified form with intestinal epithelial cells can induce a specific immunological priming necessary for the downstream activation of innate immunity. (C) 2016 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Biochimica et Biophysica Acta: General Subjects
T1  - Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells
VL  - 1861
IS  - 2
SP  - 126
EP  - 134
DO  - 10.1016/j.bbagen.2016.11.021
ER  - 
@article{
author = "Nikolić, Jasna and Nešić, Andrijana N. and Čavić, Milena and Đorđević, Neda O. and Andelković, Uroš and Atanasković-Marković, Marina and Drakulić, Branko J. and Gavrović-Jankulović, Marija",
year = "2017",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2384",
abstract = "Background: Protein oxidation can occur as a consequence of lipid peroxidation during food processing. The aim of this work was to explore the effect of malondialdehyde (MDA) modification of ovalbumin (OVA) on its interaction with T84 intestinal cells. Methods: Molecular dynamics simulation was employed for the prediction of MDA modification in the OVA, while introduced structural changes were evaluated by measurement of carbonyl group content, fluorescence spectra, MS/MS analysis, and IgE reactivity. Effects of MDA modified OVA on T84 epithelial cells were analyzed by gene expression for pro-inflammatory cytokines and protein secretion. Results: Out of 9 predicted, five modified Lys residues were confirmed by MS/MS analysis: (51)TQINKVVR(58), (85)DILNQITKPNDVYSFSLASR(104), (111)YPILPEYLQCVKELYR(126), (187)AFKDEDTQAMPFR(99), (KIKVYLPR284)-K-277, and (IKVYLPR284)-I-278. The introduced MDA modifications influenced profile of IgE reactivity to OVA. Treatment of T84 epithelial cells with OVA and OVA modified with 1 mM MDA, induced up-regulation of pro-inflammatory cytokines (IL-1 beta,IL-25, IL-33, TSLP and TNF alpha), while OVA modification with 10 mM MDA induced down regulation of the cytokine expression profile, except for IL-1 beta. OVA and OVA modified with 1 mM MDA induced secretion of epithelial cells specific cytokine IL-33. Conclusions: This finding indicated that OVA and its MDA modified form have the potential to trigger the innate immunity by inducing up-regulation and secretion of pro-allergenic IL-33 in T84 intestinal epithelial cells. General significance: Interactions of ovalbumin and its MDA modified form with intestinal epithelial cells can induce a specific immunological priming necessary for the downstream activation of innate immunity. (C) 2016 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Biochimica et Biophysica Acta: General Subjects",
title = "Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells",
volume = "1861",
number = "2",
pages = "126-134",
doi = "10.1016/j.bbagen.2016.11.021"
}
Nikolić, J., Nešić, A. N., Čavić, M., Đorđević, N. O., Andelković, U., Atanasković-Marković, M., Drakulić, B. J.,& Gavrović-Jankulović, M. (2017). Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells.
Biochimica et Biophysica Acta: General Subjects
Elsevier Science Bv, Amsterdam., 1861(2), 126-134.
https://doi.org/10.1016/j.bbagen.2016.11.021
Nikolić J, Nešić AN, Čavić M, Đorđević NO, Andelković U, Atanasković-Marković M, Drakulić BJ, Gavrović-Jankulović M. Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells. Biochimica et Biophysica Acta: General Subjects. 2017;1861(2):126-134
Nikolić Jasna, Nešić Andrijana N., Čavić Milena, Đorđević Neda O., Andelković Uroš, Atanasković-Marković Marina, Drakulić Branko J., Gavrović-Jankulović Marija, "Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells" Biochimica et Biophysica Acta: General Subjects, 1861, no. 2 (2017):126-134,
https://doi.org/10.1016/j.bbagen.2016.11.021 .
5
4
5