Weitz, David A.

Link to this page

Authority KeyName Variants
6f06ba34-baf1-4083-a7b8-3107f6c5a859
  • Weitz, David A. (9)
Projects

Author's Bibliography

A high.throughput screening system based on droplet microfluidics for glucose oxidase gene libraries

Prodanović, Radivoje; Lloyd Ung, W.; Ilić Đurđić, Karla; Fischer, Rainer; Weitz, David A.; Ostafe, Raluca

(MDPI, 2020)

TY  - JOUR
AU  - Prodanović, Radivoje
AU  - Lloyd Ung, W.
AU  - Ilić Đurđić, Karla
AU  - Fischer, Rainer
AU  - Weitz, David A.
AU  - Ostafe, Raluca
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3950
AB  - Glucose oxidase (GOx) is an important industrial enzyme that can be optimized for specific applications by mutagenesis and activity.based screening. To increase the efficiency of this approach, we have developed a new ultrahigh.throughput screening platform based on a microfluidic lab.on.chip device that allows the sorting of GOx mutants from a saturation mutagenesis library expressed on the surface of yeast cells. GOx activity was measured by monitoring the fluorescence of water microdroplets dispersed in perfluorinated oil. The signal was generated via a series of coupled enzyme reactions leading to the formation of fluorescein. Using this new method, we were able to enrich the yeast cell population by more than 35.fold for GOx mutants with higher than wild.type activity after two rounds of sorting, almost double the efficiency of our previously described flow cytometry platform. We identified and characterized novel GOx mutants, the most promising of which (M6) contained a combination of six point mutations that increased the catalytic constant kcat by 2.1.fold compared to wild.type GOx and by 1.4.fold compared to a parental GOx variant. The new microfluidic platform for GOx was therefore more sensitive than flow cytometry and supports comprehensive screens of gene libraries containing multiple mutations per gene. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
PB  - MDPI
T2  - Molecules
T1  - A high.throughput screening system based on droplet microfluidics for glucose oxidase gene libraries
VL  - 25
IS  - 10
DO  - 10.3390/molecules25102418
ER  - 
@article{
author = "Prodanović, Radivoje and Lloyd Ung, W. and Ilić Đurđić, Karla and Fischer, Rainer and Weitz, David A. and Ostafe, Raluca",
year = "2020",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3950",
abstract = "Glucose oxidase (GOx) is an important industrial enzyme that can be optimized for specific applications by mutagenesis and activity.based screening. To increase the efficiency of this approach, we have developed a new ultrahigh.throughput screening platform based on a microfluidic lab.on.chip device that allows the sorting of GOx mutants from a saturation mutagenesis library expressed on the surface of yeast cells. GOx activity was measured by monitoring the fluorescence of water microdroplets dispersed in perfluorinated oil. The signal was generated via a series of coupled enzyme reactions leading to the formation of fluorescein. Using this new method, we were able to enrich the yeast cell population by more than 35.fold for GOx mutants with higher than wild.type activity after two rounds of sorting, almost double the efficiency of our previously described flow cytometry platform. We identified and characterized novel GOx mutants, the most promising of which (M6) contained a combination of six point mutations that increased the catalytic constant kcat by 2.1.fold compared to wild.type GOx and by 1.4.fold compared to a parental GOx variant. The new microfluidic platform for GOx was therefore more sensitive than flow cytometry and supports comprehensive screens of gene libraries containing multiple mutations per gene. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).",
publisher = "MDPI",
journal = "Molecules",
title = "A high.throughput screening system based on droplet microfluidics for glucose oxidase gene libraries",
volume = "25",
number = "10",
doi = "10.3390/molecules25102418"
}
Prodanović, R., Lloyd Ung, W., Ilić Đurđić, K., Fischer, R., Weitz, D. A.,& Ostafe, R. (2020). A high.throughput screening system based on droplet microfluidics for glucose oxidase gene libraries.
Molecules
MDPI., 25(10).
https://doi.org/10.3390/molecules25102418
Prodanović R, Lloyd Ung W, Ilić Đurđić K, Fischer R, Weitz DA, Ostafe R. A high.throughput screening system based on droplet microfluidics for glucose oxidase gene libraries. Molecules. 2020;25(10)
Prodanović Radivoje, Lloyd Ung W., Ilić Đurđić Karla, Fischer Rainer, Weitz David A., Ostafe Raluca, "A high.throughput screening system based on droplet microfluidics for glucose oxidase gene libraries" Molecules, 25, no. 10 (2020),
https://doi.org/10.3390/molecules25102418 .
2
2
1
2

Supplementary data for the article: Chen, Q.; Utech, S.; Chen, D.; Prodanovic, R.; Lin, J.-M.; Weitz, D. A. Controlled Assembly of Heterotypic Cells in a Core-Shell Scaffold: Organ in a Droplet. Lab on a Chip 2016, 16 (8), 1346–1349. https://doi.org/10.1039/c6lc00231e

Chen, Qiushui; Utech, Stefanie; Chen, Dong; Prodanović, Radivoje; Lin, Jin-Ming; Weitz, David A.

(Royal Soc Chemistry, Cambridge, 2016)

TY  - BOOK
AU  - Chen, Qiushui
AU  - Utech, Stefanie
AU  - Chen, Dong
AU  - Prodanović, Radivoje
AU  - Lin, Jin-Ming
AU  - Weitz, David A.
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3427
PB  - Royal Soc Chemistry, Cambridge
T2  - Lab on A Chip
T1  - Supplementary data for the article: Chen, Q.; Utech, S.; Chen, D.; Prodanovic, R.; Lin, J.-M.; Weitz, D. A. Controlled Assembly of Heterotypic Cells in a Core-Shell Scaffold: Organ in a Droplet. Lab on a Chip 2016, 16 (8), 1346–1349. https://doi.org/10.1039/c6lc00231e
ER  - 
@book{
author = "Chen, Qiushui and Utech, Stefanie and Chen, Dong and Prodanović, Radivoje and Lin, Jin-Ming and Weitz, David A.",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3427",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Lab on A Chip",
title = "Supplementary data for the article: Chen, Q.; Utech, S.; Chen, D.; Prodanovic, R.; Lin, J.-M.; Weitz, D. A. Controlled Assembly of Heterotypic Cells in a Core-Shell Scaffold: Organ in a Droplet. Lab on a Chip 2016, 16 (8), 1346–1349. https://doi.org/10.1039/c6lc00231e"
}
Chen, Q., Utech, S., Chen, D., Prodanović, R., Lin, J.,& Weitz, D. A. (2016). Supplementary data for the article: Chen, Q.; Utech, S.; Chen, D.; Prodanovic, R.; Lin, J.-M.; Weitz, D. A. Controlled Assembly of Heterotypic Cells in a Core-Shell Scaffold: Organ in a Droplet. Lab on a Chip 2016, 16 (8), 1346–1349. https://doi.org/10.1039/c6lc00231e.
Lab on A Chip
Royal Soc Chemistry, Cambridge..
Chen Q, Utech S, Chen D, Prodanović R, Lin J, Weitz DA. Supplementary data for the article: Chen, Q.; Utech, S.; Chen, D.; Prodanovic, R.; Lin, J.-M.; Weitz, D. A. Controlled Assembly of Heterotypic Cells in a Core-Shell Scaffold: Organ in a Droplet. Lab on a Chip 2016, 16 (8), 1346–1349. https://doi.org/10.1039/c6lc00231e. Lab on A Chip. 2016;
Chen Qiushui, Utech Stefanie, Chen Dong, Prodanović Radivoje, Lin Jin-Ming, Weitz David A., "Supplementary data for the article: Chen, Q.; Utech, S.; Chen, D.; Prodanovic, R.; Lin, J.-M.; Weitz, D. A. Controlled Assembly of Heterotypic Cells in a Core-Shell Scaffold: Organ in a Droplet. Lab on a Chip 2016, 16 (8), 1346–1349. https://doi.org/10.1039/c6lc00231e" Lab on A Chip (2016)

Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet

Chen, Qiushui; Utech, Stefanie; Chen, Dong; Prodanović, Radivoje; Lin, Jin-Ming; Weitz, David A.

(Royal Soc Chemistry, Cambridge, 2016)

TY  - JOUR
AU  - Chen, Qiushui
AU  - Utech, Stefanie
AU  - Chen, Dong
AU  - Prodanović, Radivoje
AU  - Lin, Jin-Ming
AU  - Weitz, David A.
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1917
AB  - This paper reports a droplet-based microfluidic approach to fabricate a large number of monodisperse, portable microtissues, each in an individual drop. We use water-water-oil double emulsions as templates and spatially assemble hepatocytes in the core and fibroblasts in the shell, forming a 3D liver model in a drop.
PB  - Royal Soc Chemistry, Cambridge
T2  - Lab on A Chip
T1  - Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet
VL  - 16
IS  - 8
SP  - 1346
EP  - 1349
DO  - 10.1039/c6lc00231e
ER  - 
@article{
author = "Chen, Qiushui and Utech, Stefanie and Chen, Dong and Prodanović, Radivoje and Lin, Jin-Ming and Weitz, David A.",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1917",
abstract = "This paper reports a droplet-based microfluidic approach to fabricate a large number of monodisperse, portable microtissues, each in an individual drop. We use water-water-oil double emulsions as templates and spatially assemble hepatocytes in the core and fibroblasts in the shell, forming a 3D liver model in a drop.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Lab on A Chip",
title = "Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet",
volume = "16",
number = "8",
pages = "1346-1349",
doi = "10.1039/c6lc00231e"
}
Chen, Q., Utech, S., Chen, D., Prodanović, R., Lin, J.,& Weitz, D. A. (2016). Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet.
Lab on A Chip
Royal Soc Chemistry, Cambridge., 16(8), 1346-1349.
https://doi.org/10.1039/c6lc00231e
Chen Q, Utech S, Chen D, Prodanović R, Lin J, Weitz DA. Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet. Lab on A Chip. 2016;16(8):1346-1349
Chen Qiushui, Utech Stefanie, Chen Dong, Prodanović Radivoje, Lin Jin-Ming, Weitz David A., "Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet" Lab on A Chip, 16, no. 8 (2016):1346-1349,
https://doi.org/10.1039/c6lc00231e .
6
109
84
89

Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet

Chen, Qiushui; Utech, Stefanie; Chen, Dong; Prodanović, Radivoje; Lin, Jin-Ming; Weitz, David A.

(Royal Soc Chemistry, Cambridge, 2016)

TY  - JOUR
AU  - Chen, Qiushui
AU  - Utech, Stefanie
AU  - Chen, Dong
AU  - Prodanović, Radivoje
AU  - Lin, Jin-Ming
AU  - Weitz, David A.
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2820
AB  - This paper reports a droplet-based microfluidic approach to fabricate a large number of monodisperse, portable microtissues, each in an individual drop. We use water-water-oil double emulsions as templates and spatially assemble hepatocytes in the core and fibroblasts in the shell, forming a 3D liver model in a drop.
PB  - Royal Soc Chemistry, Cambridge
T2  - Lab on A Chip
T1  - Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet
VL  - 16
IS  - 8
SP  - 1346
EP  - 1349
DO  - 10.1039/c6lc00231e
ER  - 
@article{
author = "Chen, Qiushui and Utech, Stefanie and Chen, Dong and Prodanović, Radivoje and Lin, Jin-Ming and Weitz, David A.",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2820",
abstract = "This paper reports a droplet-based microfluidic approach to fabricate a large number of monodisperse, portable microtissues, each in an individual drop. We use water-water-oil double emulsions as templates and spatially assemble hepatocytes in the core and fibroblasts in the shell, forming a 3D liver model in a drop.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Lab on A Chip",
title = "Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet",
volume = "16",
number = "8",
pages = "1346-1349",
doi = "10.1039/c6lc00231e"
}
Chen, Q., Utech, S., Chen, D., Prodanović, R., Lin, J.,& Weitz, D. A. (2016). Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet.
Lab on A Chip
Royal Soc Chemistry, Cambridge., 16(8), 1346-1349.
https://doi.org/10.1039/c6lc00231e
Chen Q, Utech S, Chen D, Prodanović R, Lin J, Weitz DA. Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet. Lab on A Chip. 2016;16(8):1346-1349
Chen Qiushui, Utech Stefanie, Chen Dong, Prodanović Radivoje, Lin Jin-Ming, Weitz David A., "Controlled assembly of heterotypic cells in a core-shell scaffold: organ in a droplet" Lab on A Chip, 16, no. 8 (2016):1346-1349,
https://doi.org/10.1039/c6lc00231e .
6
109
84
90

Supplementary data for article: Utech, S.; Prodanovic, R.; Mao, A. S.; Ostafe, R.; Mooney, D. J.; Weitz, D. A. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials 2015, 4 (11), 1628–1633. https://doi.org/10.1002/adhm.201500021

Utech, Stefanie; Prodanović, Radivoje; Mao, Angelo S.; Ostafe, Raluca; Mooney, David J.; Weitz, David A.

(Wiley-Blackwell, Hoboken, 2015)

TY  - BOOK
AU  - Utech, Stefanie
AU  - Prodanović, Radivoje
AU  - Mao, Angelo S.
AU  - Ostafe, Raluca
AU  - Mooney, David J.
AU  - Weitz, David A.
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3383
PB  - Wiley-Blackwell, Hoboken
T2  - Advanced Healthcare Materials
T1  - Supplementary data for article: Utech, S.; Prodanovic, R.; Mao, A. S.; Ostafe, R.; Mooney, D. J.; Weitz, D. A. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials 2015, 4 (11), 1628–1633. https://doi.org/10.1002/adhm.201500021
ER  - 
@book{
author = "Utech, Stefanie and Prodanović, Radivoje and Mao, Angelo S. and Ostafe, Raluca and Mooney, David J. and Weitz, David A.",
year = "2015",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3383",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Advanced Healthcare Materials",
title = "Supplementary data for article: Utech, S.; Prodanovic, R.; Mao, A. S.; Ostafe, R.; Mooney, D. J.; Weitz, D. A. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials 2015, 4 (11), 1628–1633. https://doi.org/10.1002/adhm.201500021"
}
Utech, S., Prodanović, R., Mao, A. S., Ostafe, R., Mooney, D. J.,& Weitz, D. A. (2015). Supplementary data for article: Utech, S.; Prodanovic, R.; Mao, A. S.; Ostafe, R.; Mooney, D. J.; Weitz, D. A. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials 2015, 4 (11), 1628–1633. https://doi.org/10.1002/adhm.201500021.
Advanced Healthcare Materials
Wiley-Blackwell, Hoboken..
Utech S, Prodanović R, Mao AS, Ostafe R, Mooney DJ, Weitz DA. Supplementary data for article: Utech, S.; Prodanovic, R.; Mao, A. S.; Ostafe, R.; Mooney, D. J.; Weitz, D. A. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials 2015, 4 (11), 1628–1633. https://doi.org/10.1002/adhm.201500021. Advanced Healthcare Materials. 2015;
Utech Stefanie, Prodanović Radivoje, Mao Angelo S., Ostafe Raluca, Mooney David J., Weitz David A., "Supplementary data for article: Utech, S.; Prodanovic, R.; Mao, A. S.; Ostafe, R.; Mooney, D. J.; Weitz, D. A. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials 2015, 4 (11), 1628–1633. https://doi.org/10.1002/adhm.201500021" Advanced Healthcare Materials (2015)

Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture

Utech, Stefanie; Prodanović, Radivoje; Mao, Angelo S.; Ostafe, Raluca; Mooney, David J.; Weitz, David A.

(Wiley-Blackwell, Hoboken, 2015)

TY  - JOUR
AU  - Utech, Stefanie
AU  - Prodanović, Radivoje
AU  - Mao, Angelo S.
AU  - Ostafe, Raluca
AU  - Mooney, David J.
AU  - Weitz, David A.
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2785
AB  - Monodisperse alginate microgels (10–50 μm) are created via droplet‐based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments.
PB  - Wiley-Blackwell, Hoboken
T2  - Advanced Healthcare Materials
T1  - Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture
VL  - 4
IS  - 11
SP  - 1628
EP  - 1633
DO  - 10.1002/adhm.201500021
ER  - 
@article{
author = "Utech, Stefanie and Prodanović, Radivoje and Mao, Angelo S. and Ostafe, Raluca and Mooney, David J. and Weitz, David A.",
year = "2015",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2785",
abstract = "Monodisperse alginate microgels (10–50 μm) are created via droplet‐based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Advanced Healthcare Materials",
title = "Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture",
volume = "4",
number = "11",
pages = "1628-1633",
doi = "10.1002/adhm.201500021"
}
Utech, S., Prodanović, R., Mao, A. S., Ostafe, R., Mooney, D. J.,& Weitz, D. A. (2015). Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture.
Advanced Healthcare Materials
Wiley-Blackwell, Hoboken., 4(11), 1628-1633.
https://doi.org/10.1002/adhm.201500021
Utech S, Prodanović R, Mao AS, Ostafe R, Mooney DJ, Weitz DA. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials. 2015;4(11):1628-1633
Utech Stefanie, Prodanović Radivoje, Mao Angelo S., Ostafe Raluca, Mooney David J., Weitz David A., "Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture" Advanced Healthcare Materials, 4, no. 11 (2015):1628-1633,
https://doi.org/10.1002/adhm.201500021 .
176
145
165

Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture

Utech, Stefanie; Prodanović, Radivoje; Mao, Angelo S.; Ostafe, Raluca; Mooney, David J.; Weitz, David A.

(Wiley-Blackwell, Hoboken, 2015)

TY  - JOUR
AU  - Utech, Stefanie
AU  - Prodanović, Radivoje
AU  - Mao, Angelo S.
AU  - Ostafe, Raluca
AU  - Mooney, David J.
AU  - Weitz, David A.
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1751
PB  - Wiley-Blackwell, Hoboken
T2  - Advanced Healthcare Materials
T1  - Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture
VL  - 4
IS  - 11
SP  - 1628
EP  - 1633
DO  - 10.1002/adhm.201500021
ER  - 
@article{
author = "Utech, Stefanie and Prodanović, Radivoje and Mao, Angelo S. and Ostafe, Raluca and Mooney, David J. and Weitz, David A.",
year = "2015",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1751",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Advanced Healthcare Materials",
title = "Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture",
volume = "4",
number = "11",
pages = "1628-1633",
doi = "10.1002/adhm.201500021"
}
Utech, S., Prodanović, R., Mao, A. S., Ostafe, R., Mooney, D. J.,& Weitz, D. A. (2015). Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture.
Advanced Healthcare Materials
Wiley-Blackwell, Hoboken., 4(11), 1628-1633.
https://doi.org/10.1002/adhm.201500021
Utech S, Prodanović R, Mao AS, Ostafe R, Mooney DJ, Weitz DA. Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture. Advanced Healthcare Materials. 2015;4(11):1628-1633
Utech Stefanie, Prodanović Radivoje, Mao Angelo S., Ostafe Raluca, Mooney David J., Weitz David A., "Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture" Advanced Healthcare Materials, 4, no. 11 (2015):1628-1633,
https://doi.org/10.1002/adhm.201500021 .
176
145
162

Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771

Ostafe, Raluca; Prodanović, Radivoje; Ung, Lloyd W.; Weitz, David A.; Fischer, Rainer

(Amer Inst Physics, Melville, 2014)

TY  - BOOK
AU  - Ostafe, Raluca
AU  - Prodanović, Radivoje
AU  - Ung, Lloyd W.
AU  - Weitz, David A.
AU  - Fischer, Rainer
PY  - 2014
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3666
PB  - Amer Inst Physics, Melville
T2  - Biomicrofluidics
T1  - Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771
ER  - 
@book{
author = "Ostafe, Raluca and Prodanović, Radivoje and Ung, Lloyd W. and Weitz, David A. and Fischer, Rainer",
year = "2014",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3666",
publisher = "Amer Inst Physics, Melville",
journal = "Biomicrofluidics",
title = "Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771"
}
Ostafe, R., Prodanović, R., Ung, L. W., Weitz, D. A.,& Fischer, R. (2014). Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771.
Biomicrofluidics
Amer Inst Physics, Melville..
Ostafe R, Prodanović R, Ung LW, Weitz DA, Fischer R. Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771. Biomicrofluidics. 2014;
Ostafe Raluca, Prodanović Radivoje, Ung Lloyd W., Weitz David A., Fischer Rainer, "Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771" Biomicrofluidics (2014)

A high-throughput cellulase screening system based on droplet microfluidics

Ostafe, Raluca; Prodanović, Radivoje; Ung, Lloyd W.; Weitz, David A.; Fischer, Rainer

(Amer Inst Physics, Melville, 2014)

TY  - JOUR
AU  - Ostafe, Raluca
AU  - Prodanović, Radivoje
AU  - Ung, Lloyd W.
AU  - Weitz, David A.
AU  - Fischer, Rainer
PY  - 2014
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1873
AB  - A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells. (C) 2014 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.
PB  - Amer Inst Physics, Melville
T2  - Biomicrofluidics
T1  - A high-throughput cellulase screening system based on droplet microfluidics
VL  - 8
IS  - 4
DO  - 10.1063/1.4886771
ER  - 
@article{
author = "Ostafe, Raluca and Prodanović, Radivoje and Ung, Lloyd W. and Weitz, David A. and Fischer, Rainer",
year = "2014",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1873",
abstract = "A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells. (C) 2014 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.",
publisher = "Amer Inst Physics, Melville",
journal = "Biomicrofluidics",
title = "A high-throughput cellulase screening system based on droplet microfluidics",
volume = "8",
number = "4",
doi = "10.1063/1.4886771"
}
Ostafe, R., Prodanović, R., Ung, L. W., Weitz, D. A.,& Fischer, R. (2014). A high-throughput cellulase screening system based on droplet microfluidics.
Biomicrofluidics
Amer Inst Physics, Melville., 8(4).
https://doi.org/10.1063/1.4886771
Ostafe R, Prodanović R, Ung LW, Weitz DA, Fischer R. A high-throughput cellulase screening system based on droplet microfluidics. Biomicrofluidics. 2014;8(4)
Ostafe Raluca, Prodanović Radivoje, Ung Lloyd W., Weitz David A., Fischer Rainer, "A high-throughput cellulase screening system based on droplet microfluidics" Biomicrofluidics, 8, no. 4 (2014),
https://doi.org/10.1063/1.4886771 .
4
44
34
38