TY  - JOUR
AU  - Lozano-Ojalvo, Daniel
AU  - Benedé, Sara
AU  - Antunes, Celia M.
AU  - Bavaro, Simona L.
AU  - Bouchaud, Grégory
AU  - Costa, Ana
AU  - Denery-Papini, Sandra
AU  - Díaz-Perales, Araceli
AU  - Garrido-Arandia, María
AU  - Gavrović-Jankulović, Marija
AU  - Hayen, Simone
AU  - Martínez-Blanco, Mónica
AU  - Molina, Elena
AU  - Monaci, Linda
AU  - Pieters, Raymond
AU  - Villemin, Clelia
AU  - Wichers, Harry J.
AU  - Wróblewska, Barbara
AU  - Willemsen, Linette E.M.
AU  - Roggen, Erwin L.
AU  - van Bilsen, Jolanda H.M.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2864
AB  - Background: Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. Scope and approach: In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. Key findings and conclusions: The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches.
PB  - Elsevier
T2  - Trends in Food Science and Technology
T1  - Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies
VL  - 85
SP  - 307
EP  - 319
DO  - 10.1016/j.tifs.2019.01.014
ER  - 

@article{
author = "Lozano-Ojalvo, Daniel and Benedé, Sara and Antunes, Celia M. and Bavaro, Simona L. and Bouchaud, Grégory and Costa, Ana and Denery-Papini, Sandra and Díaz-Perales, Araceli and Garrido-Arandia, María and Gavrović-Jankulović, Marija and Hayen, Simone and Martínez-Blanco, Mónica and Molina, Elena and Monaci, Linda and Pieters, Raymond and Villemin, Clelia and Wichers, Harry J. and Wróblewska, Barbara and Willemsen, Linette E.M. and Roggen, Erwin L. and van Bilsen, Jolanda H.M.",
year = "2019",
abstract = "Background: Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. Scope and approach: In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. Key findings and conclusions: The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches.",
publisher = "Elsevier",
journal = "Trends in Food Science and Technology",
title = "Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies",
volume = "85",
pages = "307-319",
doi = "10.1016/j.tifs.2019.01.014"
}

Lozano-Ojalvo, D., Benedé, S., Antunes, C. M., Bavaro, S. L., Bouchaud, G., Costa, A., Denery-Papini, S., Díaz-Perales, A., Garrido-Arandia, M., Gavrović-Jankulović, M., Hayen, S., Martínez-Blanco, M., Molina, E., Monaci, L., Pieters, R., Villemin, C., Wichers, H. J., Wróblewska, B., Willemsen, L. E.M., Roggen, E. L.,& van Bilsen, J. H.M.. (2019). Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies. in Trends in Food Science and Technology
Elsevier., 85, 307-319.
https://doi.org/10.1016/j.tifs.2019.01.014

Lozano-Ojalvo D, Benedé S, Antunes CM, Bavaro SL, Bouchaud G, Costa A, Denery-Papini S, Díaz-Perales A, Garrido-Arandia M, Gavrović-Jankulović M, Hayen S, Martínez-Blanco M, Molina E, Monaci L, Pieters R, Villemin C, Wichers HJ, Wróblewska B, Willemsen LE, Roggen EL, van Bilsen JH. Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies. in Trends in Food Science and Technology. 2019;85:307-319.
doi:10.1016/j.tifs.2019.01.014 .

Lozano-Ojalvo, Daniel, Benedé, Sara, Antunes, Celia M., Bavaro, Simona L., Bouchaud, Grégory, Costa, Ana, Denery-Papini, Sandra, Díaz-Perales, Araceli, Garrido-Arandia, María, Gavrović-Jankulović, Marija, Hayen, Simone, Martínez-Blanco, Mónica, Molina, Elena, Monaci, Linda, Pieters, Raymond, Villemin, Clelia, Wichers, Harry J., Wróblewska, Barbara, Willemsen, Linette E.M., Roggen, Erwin L., van Bilsen, Jolanda H.M., "Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies" in Trends in Food Science and Technology, 85 (2019):307-319,
https://doi.org/10.1016/j.tifs.2019.01.014 . .

TY  - JOUR
AU  - Lozano-Ojalvo, Daniel
AU  - Benedé, Sara
AU  - Antunes, Celia M.
AU  - Bavaro, Simona L.
AU  - Bouchaud, Grégory
AU  - Costa, Ana
AU  - Denery-Papini, Sandra
AU  - Díaz-Perales, Araceli
AU  - Garrido-Arandia, María
AU  - Gavrović-Jankulović, Marija
AU  - Hayen, Simone
AU  - Martínez-Blanco, Mónica
AU  - Molina, Elena
AU  - Monaci, Linda
AU  - Pieters, Raymond
AU  - Villemin, Clelia
AU  - Wichers, Harry J.
AU  - Wróblewska, Barbara
AU  - Willemsen, Linette E.M.
AU  - Roggen, Erwin L.
AU  - van Bilsen, Jolanda H.M.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2852
AB  - Background: Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. Scope and approach: In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. Key findings and conclusions: The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches.
PB  - Elsevier
T2  - Trends in Food Science and Technology
T1  - Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies
VL  - 85
SP  - 307
EP  - 319
DO  - 10.1016/j.tifs.2019.01.014
ER  - 

@article{
author = "Lozano-Ojalvo, Daniel and Benedé, Sara and Antunes, Celia M. and Bavaro, Simona L. and Bouchaud, Grégory and Costa, Ana and Denery-Papini, Sandra and Díaz-Perales, Araceli and Garrido-Arandia, María and Gavrović-Jankulović, Marija and Hayen, Simone and Martínez-Blanco, Mónica and Molina, Elena and Monaci, Linda and Pieters, Raymond and Villemin, Clelia and Wichers, Harry J. and Wróblewska, Barbara and Willemsen, Linette E.M. and Roggen, Erwin L. and van Bilsen, Jolanda H.M.",
year = "2019",
abstract = "Background: Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. Scope and approach: In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. Key findings and conclusions: The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches.",
publisher = "Elsevier",
journal = "Trends in Food Science and Technology",
title = "Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies",
volume = "85",
pages = "307-319",
doi = "10.1016/j.tifs.2019.01.014"
}

Lozano-Ojalvo, D., Benedé, S., Antunes, C. M., Bavaro, S. L., Bouchaud, G., Costa, A., Denery-Papini, S., Díaz-Perales, A., Garrido-Arandia, M., Gavrović-Jankulović, M., Hayen, S., Martínez-Blanco, M., Molina, E., Monaci, L., Pieters, R., Villemin, C., Wichers, H. J., Wróblewska, B., Willemsen, L. E.M., Roggen, E. L.,& van Bilsen, J. H.M.. (2019). Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies. in Trends in Food Science and Technology
Elsevier., 85, 307-319.
https://doi.org/10.1016/j.tifs.2019.01.014

Lozano-Ojalvo D, Benedé S, Antunes CM, Bavaro SL, Bouchaud G, Costa A, Denery-Papini S, Díaz-Perales A, Garrido-Arandia M, Gavrović-Jankulović M, Hayen S, Martínez-Blanco M, Molina E, Monaci L, Pieters R, Villemin C, Wichers HJ, Wróblewska B, Willemsen LE, Roggen EL, van Bilsen JH. Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies. in Trends in Food Science and Technology. 2019;85:307-319.
doi:10.1016/j.tifs.2019.01.014 .

Lozano-Ojalvo, Daniel, Benedé, Sara, Antunes, Celia M., Bavaro, Simona L., Bouchaud, Grégory, Costa, Ana, Denery-Papini, Sandra, Díaz-Perales, Araceli, Garrido-Arandia, María, Gavrović-Jankulović, Marija, Hayen, Simone, Martínez-Blanco, Mónica, Molina, Elena, Monaci, Linda, Pieters, Raymond, Villemin, Clelia, Wichers, Harry J., Wróblewska, Barbara, Willemsen, Linette E.M., Roggen, Erwin L., van Bilsen, Jolanda H.M., "Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies" in Trends in Food Science and Technology, 85 (2019):307-319,
https://doi.org/10.1016/j.tifs.2019.01.014 . .

TY  - JOUR
AU  - Cubells-Baeza, N.
AU  - Verhoeckx, K.C.M.
AU  - Larre, C.
AU  - Denery-Papini, Sandra
AU  - Gavrović-Jankulović, Marija
AU  - Diaz Perales, A.
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/304
AB  - Measurement of protein transport across the intestinal barrier might be a relevant approach in allergenicity risk assessment. Traditionally, studies on protein transport, were performed using stable cell lines cultured as a monolayer. One of the major advantages of these models is their relatively low price and easy handling. However, monolayers lack a physiologically relevant environment (presence of other cell-types and a mucus layer), which may have an effect on transport characteristics and thus correct prediction of protein allergenicity. This paper summarizes the most widely used epithelial models and discusses their benefits and limitations for measuring protein transport and allergic sensitization to food. © 2016 Elsevier Ltd
T2  - Drug Discovery Today: Disease Models
T1  - Applicability of epithelial models in protein permeability/transport studies and food allergy
SP  - 13
EP  - 21
DO  - 10.1016/j.ddmod.2016.08.002
ER  - 

@article{
author = "Cubells-Baeza, N. and Verhoeckx, K.C.M. and Larre, C. and Denery-Papini, Sandra and Gavrović-Jankulović, Marija and Diaz Perales, A.",
year = "2015",
abstract = "Measurement of protein transport across the intestinal barrier might be a relevant approach in allergenicity risk assessment. Traditionally, studies on protein transport, were performed using stable cell lines cultured as a monolayer. One of the major advantages of these models is their relatively low price and easy handling. However, monolayers lack a physiologically relevant environment (presence of other cell-types and a mucus layer), which may have an effect on transport characteristics and thus correct prediction of protein allergenicity. This paper summarizes the most widely used epithelial models and discusses their benefits and limitations for measuring protein transport and allergic sensitization to food. © 2016 Elsevier Ltd",
journal = "Drug Discovery Today: Disease Models",
title = "Applicability of epithelial models in protein permeability/transport studies and food allergy",
pages = "13-21",
doi = "10.1016/j.ddmod.2016.08.002"
}

Cubells-Baeza, N., Verhoeckx, K.C.M., Larre, C., Denery-Papini, S., Gavrović-Jankulović, M.,& Diaz Perales, A.. (2015). Applicability of epithelial models in protein permeability/transport studies and food allergy. in Drug Discovery Today: Disease Models, 13-21.
https://doi.org/10.1016/j.ddmod.2016.08.002

Cubells-Baeza N, Verhoeckx K, Larre C, Denery-Papini S, Gavrović-Jankulović M, Diaz Perales A. Applicability of epithelial models in protein permeability/transport studies and food allergy. in Drug Discovery Today: Disease Models. 2015;:13-21.
doi:10.1016/j.ddmod.2016.08.002 .

Cubells-Baeza, N., Verhoeckx, K.C.M., Larre, C., Denery-Papini, Sandra, Gavrović-Jankulović, Marija, Diaz Perales, A., "Applicability of epithelial models in protein permeability/transport studies and food allergy" in Drug Discovery Today: Disease Models (2015):13-21,
https://doi.org/10.1016/j.ddmod.2016.08.002 . .