TY  - JOUR
AU  - Descamps, Julie
AU  - Zhao, Yiran
AU  - Goudeau, Bertrand
AU  - Manojlović, Dragan D.
AU  - Loget, Gabriel
AU  - Šojić, Nešo
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6441
AB  - Electrochemiluminescence (ECL) is evolving rapidly from a purely analytical technique into a powerful microscopy. Herein, we report the imaging of single cells by photoinduced ECL (PECL; λem = 620 nm) stimulated by an incident near-infrared light (λexc = 1050 nm). The cells were grown on a metal–insulator–semiconductor (MIS) n-Si/SiOx/Ir photoanode that exhibited stable and bright PECL emission. The large anti-Stokes shift allowed for the recording of well-resolved images of cells with high sensitivity. PECL microscopy is demonstrated at a remarkably low onset potential of 0.8 V; this contrasts with classic ECL, which is blind at this potential. Two imaging modes are reported: (i) photoinduced positive ECL (PECL+), showing the cell membranes labeled with the [Ru(bpy)3]2+ complex; and (ii) photoinduced shadow label-free ECL (PECL−) of cell morphology, with the luminophore in the solution. Finally, by adding a new dimension with the near-infrared light stimulus, PECL microscopy should find promising applications to image and study single photoactive nanoparticles and biological entities.
PB  - Royal Society of Chemistry
T2  - Chemical Science
T1  - Infrared photoinduced electrochemiluminescence microscopy of single cells
VL  - 15
IS  - 6
SP  - 2055
EP  - 2061
DO  - 10.1039/D3SC05983A
ER  - 

@article{
author = "Descamps, Julie and Zhao, Yiran and Goudeau, Bertrand and Manojlović, Dragan D. and Loget, Gabriel and Šojić, Nešo",
year = "2024",
abstract = "Electrochemiluminescence (ECL) is evolving rapidly from a purely analytical technique into a powerful microscopy. Herein, we report the imaging of single cells by photoinduced ECL (PECL; λem = 620 nm) stimulated by an incident near-infrared light (λexc = 1050 nm). The cells were grown on a metal–insulator–semiconductor (MIS) n-Si/SiOx/Ir photoanode that exhibited stable and bright PECL emission. The large anti-Stokes shift allowed for the recording of well-resolved images of cells with high sensitivity. PECL microscopy is demonstrated at a remarkably low onset potential of 0.8 V; this contrasts with classic ECL, which is blind at this potential. Two imaging modes are reported: (i) photoinduced positive ECL (PECL+), showing the cell membranes labeled with the [Ru(bpy)3]2+ complex; and (ii) photoinduced shadow label-free ECL (PECL−) of cell morphology, with the luminophore in the solution. Finally, by adding a new dimension with the near-infrared light stimulus, PECL microscopy should find promising applications to image and study single photoactive nanoparticles and biological entities.",
publisher = "Royal Society of Chemistry",
journal = "Chemical Science",
title = "Infrared photoinduced electrochemiluminescence microscopy of single cells",
volume = "15",
number = "6",
pages = "2055-2061",
doi = "10.1039/D3SC05983A"
}

Descamps, J., Zhao, Y., Goudeau, B., Manojlović, D. D., Loget, G.,& Šojić, N.. (2024). Infrared photoinduced electrochemiluminescence microscopy of single cells. in Chemical Science
Royal Society of Chemistry., 15(6), 2055-2061.
https://doi.org/10.1039/D3SC05983A

Descamps J, Zhao Y, Goudeau B, Manojlović DD, Loget G, Šojić N. Infrared photoinduced electrochemiluminescence microscopy of single cells. in Chemical Science. 2024;15(6):2055-2061.
doi:10.1039/D3SC05983A .

Descamps, Julie, Zhao, Yiran, Goudeau, Bertrand, Manojlović, Dragan D., Loget, Gabriel, Šojić, Nešo, "Infrared photoinduced electrochemiluminescence microscopy of single cells" in Chemical Science, 15, no. 6 (2024):2055-2061,
https://doi.org/10.1039/D3SC05983A . .

TY  - JOUR
AU  - Sentić, Milica
AU  - Arbault, Stephane
AU  - Goudeau, Bertrand
AU  - Manojlović, Dragan D.
AU  - Kuhn, Alexander
AU  - Bouffier, Laurent
AU  - Šojić, Nešo
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1834
AB  - An electrochemiluminescent (ECL) swimmer driven by bipolar electrochemistry is reported for enzymatic glucose sensing. The chemomechanical motion is induced by localized hydrogen bubble generation. The concomitant oxidation of the luminophore and of the enzymatically-produced NADH leads to ECL emission with a direct glucose-dependent light intensity. We demonstrate herein the local sensing and reporting of glucose in a concentration gradient explored by the ECL swimmer. Such a dynamic sensing approach combines in a synergetic way the wireless propulsion with the enzymatic selectivity using ECL as a readout method at the level of moving objects.
PB  - Royal Soc Chemistry, Cambridge
T2  - Chemical Communications
T1  - Electrochemiluminescent swimmers for dynamic enzymatic sensing
VL  - 50
IS  - 71
SP  - 10202
EP  - 10205
DO  - 10.1039/c4cc04105d
ER  - 

@article{
author = "Sentić, Milica and Arbault, Stephane and Goudeau, Bertrand and Manojlović, Dragan D. and Kuhn, Alexander and Bouffier, Laurent and Šojić, Nešo",
year = "2014",
abstract = "An electrochemiluminescent (ECL) swimmer driven by bipolar electrochemistry is reported for enzymatic glucose sensing. The chemomechanical motion is induced by localized hydrogen bubble generation. The concomitant oxidation of the luminophore and of the enzymatically-produced NADH leads to ECL emission with a direct glucose-dependent light intensity. We demonstrate herein the local sensing and reporting of glucose in a concentration gradient explored by the ECL swimmer. Such a dynamic sensing approach combines in a synergetic way the wireless propulsion with the enzymatic selectivity using ECL as a readout method at the level of moving objects.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Chemical Communications",
title = "Electrochemiluminescent swimmers for dynamic enzymatic sensing",
volume = "50",
number = "71",
pages = "10202-10205",
doi = "10.1039/c4cc04105d"
}

Sentić, M., Arbault, S., Goudeau, B., Manojlović, D. D., Kuhn, A., Bouffier, L.,& Šojić, N.. (2014). Electrochemiluminescent swimmers for dynamic enzymatic sensing. in Chemical Communications
Royal Soc Chemistry, Cambridge., 50(71), 10202-10205.
https://doi.org/10.1039/c4cc04105d

Sentić M, Arbault S, Goudeau B, Manojlović DD, Kuhn A, Bouffier L, Šojić N. Electrochemiluminescent swimmers for dynamic enzymatic sensing. in Chemical Communications. 2014;50(71):10202-10205.
doi:10.1039/c4cc04105d .

Sentić, Milica, Arbault, Stephane, Goudeau, Bertrand, Manojlović, Dragan D., Kuhn, Alexander, Bouffier, Laurent, Šojić, Nešo, "Electrochemiluminescent swimmers for dynamic enzymatic sensing" in Chemical Communications, 50, no. 71 (2014):10202-10205,
https://doi.org/10.1039/c4cc04105d . .