TY  - JOUR
AU  - Jurišević, Milena
AU  - Jagić, Nikola
AU  - Gajović, Nevena
AU  - Arsenijević, Aleksandar
AU  - Jovanović, Milan
AU  - Milovanović, Marija
AU  - Pantić, Jelena
AU  - Jovanović, Ivan
AU  - Sabo, Tibor
AU  - Radosavljević, Gordana
AU  - Arsenijević, Nebojša
PY  - 2020
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4238
AB  - Background/Aim. O,O'-diethyl-(S,S)-ethylenediamineN,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride (DE-EDCP) has been found to possess promising cytotoxic activity against various tumor cell lines. Also, DE-EDCP reduces tumor progression by several mechanisms such as triggering tumor cell death and inhibition of cell proliferation. The aim of present study was to further evaluate antitumor activity of DE-EDCP by investigating effects on migratory potential of tumor cells and anti-tumor immune response. Methods. Migratory potential of DE-EDCP was evaluated by scratch wound assay. Female BALB/c mice were inoculated with 4T1 breast cancer cells and treatment with DE-EDCP started five days following orthotopic tumor implantation. The frequency and phenotype of tumor-infiltrating natural killer (NK) and natural killer T (NKT) cells were analyzed by flow cytometry. Results. DE-EDCP inhibited migratory potential of highly metastatic 4T1 cells. DE-EDCP facilitated accumulation of CD3+CD49+ NKT cells and CD3-CD49+ NK cells in tumor microenvironment. DE-EDCP treatment led to significant decrement of tumor infiltrating anergic NKT cells expressing cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), killer cell lectin like receptor G1 (KLRG-1) and programmed cell death protein-1 (PD-1). Mice given DE-EDCP had significantly increased percentages of tumoricidal fas ligand (FasL) positive NK cells. Conclusion. DE-EDCP inhibits murine breast cancer progression through direct effects on tumor cells and by facilitating anti-tumor immunity. DE-EDCP enhances accumulation, promotes tumoricidal phenotype and maintenances responsiveness of NK and NKT cells in 4T1 murine breast cancer.
PB  - Military Medical Academy, INI
T2  - Vojnosanitetski pregled
T1  - O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer
VL  - 77
IS  - 7
SP  - 715
EP  - 723
DO  - 10.2298/VSP180723149J
ER  - 

@article{
author = "Jurišević, Milena and Jagić, Nikola and Gajović, Nevena and Arsenijević, Aleksandar and Jovanović, Milan and Milovanović, Marija and Pantić, Jelena and Jovanović, Ivan and Sabo, Tibor and Radosavljević, Gordana and Arsenijević, Nebojša",
year = "2020",
abstract = "Background/Aim. O,O'-diethyl-(S,S)-ethylenediamineN,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride (DE-EDCP) has been found to possess promising cytotoxic activity against various tumor cell lines. Also, DE-EDCP reduces tumor progression by several mechanisms such as triggering tumor cell death and inhibition of cell proliferation. The aim of present study was to further evaluate antitumor activity of DE-EDCP by investigating effects on migratory potential of tumor cells and anti-tumor immune response. Methods. Migratory potential of DE-EDCP was evaluated by scratch wound assay. Female BALB/c mice were inoculated with 4T1 breast cancer cells and treatment with DE-EDCP started five days following orthotopic tumor implantation. The frequency and phenotype of tumor-infiltrating natural killer (NK) and natural killer T (NKT) cells were analyzed by flow cytometry. Results. DE-EDCP inhibited migratory potential of highly metastatic 4T1 cells. DE-EDCP facilitated accumulation of CD3+CD49+ NKT cells and CD3-CD49+ NK cells in tumor microenvironment. DE-EDCP treatment led to significant decrement of tumor infiltrating anergic NKT cells expressing cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), killer cell lectin like receptor G1 (KLRG-1) and programmed cell death protein-1 (PD-1). Mice given DE-EDCP had significantly increased percentages of tumoricidal fas ligand (FasL) positive NK cells. Conclusion. DE-EDCP inhibits murine breast cancer progression through direct effects on tumor cells and by facilitating anti-tumor immunity. DE-EDCP enhances accumulation, promotes tumoricidal phenotype and maintenances responsiveness of NK and NKT cells in 4T1 murine breast cancer.",
publisher = "Military Medical Academy, INI",
journal = "Vojnosanitetski pregled",
title = "O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer",
volume = "77",
number = "7",
pages = "715-723",
doi = "10.2298/VSP180723149J"
}

Jurišević, M., Jagić, N., Gajović, N., Arsenijević, A., Jovanović, M., Milovanović, M., Pantić, J., Jovanović, I., Sabo, T., Radosavljević, G.,& Arsenijević, N.. (2020). O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer. in Vojnosanitetski pregled
Military Medical Academy, INI., 77(7), 715-723.
https://doi.org/10.2298/VSP180723149J

Jurišević M, Jagić N, Gajović N, Arsenijević A, Jovanović M, Milovanović M, Pantić J, Jovanović I, Sabo T, Radosavljević G, Arsenijević N. O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer. in Vojnosanitetski pregled. 2020;77(7):715-723.
doi:10.2298/VSP180723149J .

Jurišević, Milena, Jagić, Nikola, Gajović, Nevena, Arsenijević, Aleksandar, Jovanović, Milan, Milovanović, Marija, Pantić, Jelena, Jovanović, Ivan, Sabo, Tibor, Radosavljević, Gordana, Arsenijević, Nebojša, "O,O'-diethyl-(S,S)-ethylenediamine-N,N'-di-2-(3-cyclohexyl)propanoate dihydrochloride enhances influx of effective NK and NKT cells in murine breast cancer" in Vojnosanitetski pregled, 77, no. 7 (2020):715-723,
https://doi.org/10.2298/VSP180723149J . .

TY  - JOUR
AU  - Jurišević, Milena
AU  - Radosavljević, Gordana
AU  - Arsenijević, Aleksandar
AU  - Milovanović, Marija
AU  - Gajović, Nevena
AU  - Đorđević, Dragana S.
AU  - Milovanović, Jelena
AU  - Stojanović, Bojana
AU  - Ilić, Aleksandar
AU  - Sabo, Tibor
AU  - Kanjevac, Tatjana
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/216
AB  - e design of platinum based drugs is not a new field of interest. Platinum complexes are widely used as anticancer agents and currently, approximately 30 platinum(II) and platinum(IV) entered into some of the phases of clinical trials. A special place in today’s research belongs to platinum complexes with diammine ligands. A large number of edda (ethylenediamine- N, N’-diacetate)-type ligands and their corresponding metal complexes has been successfully synthesized. is article summarizes recent progress in research on edda-type-platinum complexes. Some of these agents achieves better effect compared to the gold standard (cisplatin). It has been shown that there is a possible relationship between the length of the ligand ester group carbon chain and its cytotoxic effect. In most cases the longer the ester chain is the greater is the antitumor activity. Of particular interest are the noticeable effects of some new platinum compound with edda-type ligand on cell lines that are known to have a high level of cisplatin-resistance. Exanimate complexes appear to have a diff erent mode of mechanism of action compared with cisplatin which includes apoptotic and necrotic cell death. ere are indications that further investigations of these compounds may be very useful in overcoming the problems associated global cancer statistic. © 2016, University of Kragujevac, Faculty of Science. All rights reserved.
AB  - Kompleski platine koriste se kao osnova za dizajn novih lekova. Oni su u širokoj upotrebi kao antitumorski agensi i do danas je oko 30 komplesa platine(II) i platine(IV) u nekoj od faza kliničkog ispitivanja. Posebno mesto u današnjim istaživanjima zauzimaju kompleksi metala sa edda ligandima. Uspešno je sintetisan veliki broj novih edda liganda i odgovarajućih kompleksa. Neki od ovih agensa pokazuju bolju aktivnost od zlatnog standarda, cisplatine. Pokazano je da postoji moguća veza između dužine ugljovodiničnog lanca estraske grupe liganda i citotoksičnog efekta. U većini slučajeva dužina lanca direktno korelira sa antitumorskom aktivnošću. Zabeležena je efikasnija citotoksicna aktivnost određenih kompleska platine sa edda ligandima na ćelijskim linijama tumora koji pokazuju odgovarajući stepen rezistencije na cisplatinu. Ispitivani komleksi imaju različit mehanizam dejstva od cisplatine, koji uključuje elemente nekrotične i programirane ćelijske smrti. Postoje nagoveštaji da dalja istraživanja ovih agensa mogu biti značajna za prevazilaženje globalnog problema sa kojim se svet danas suočava, a koji se odnosi na stalni porast osoba obolelih od karcinoma.
T2  - Serbian Journal of Experimental and Clinical Research (ranije: Medicus)
T1  - Platinum complexes with edda ethylenediamine n, n diacetate ligands as potential anticancer agents [Kompleksi platine sa edda etilendiamin N, N’ diacetat ligandima kao potencijalni antitumorski agensi]
T1  - Kompleksi platine sa edda (etilendiamin-N, N'-diacetat) ligandima kao potencijalni antitumorski agensi
VL  - 17
IS  - 4
SP  - 285
EP  - 295
DO  - 10.1515/SJECR-2016-0042
ER  - 

@article{
author = "Jurišević, Milena and Radosavljević, Gordana and Arsenijević, Aleksandar and Milovanović, Marija and Gajović, Nevena and Đorđević, Dragana S. and Milovanović, Jelena and Stojanović, Bojana and Ilić, Aleksandar and Sabo, Tibor and Kanjevac, Tatjana",
year = "2016",
abstract = "e design of platinum based drugs is not a new field of interest. Platinum complexes are widely used as anticancer agents and currently, approximately 30 platinum(II) and platinum(IV) entered into some of the phases of clinical trials. A special place in today’s research belongs to platinum complexes with diammine ligands. A large number of edda (ethylenediamine- N, N’-diacetate)-type ligands and their corresponding metal complexes has been successfully synthesized. is article summarizes recent progress in research on edda-type-platinum complexes. Some of these agents achieves better effect compared to the gold standard (cisplatin). It has been shown that there is a possible relationship between the length of the ligand ester group carbon chain and its cytotoxic effect. In most cases the longer the ester chain is the greater is the antitumor activity. Of particular interest are the noticeable effects of some new platinum compound with edda-type ligand on cell lines that are known to have a high level of cisplatin-resistance. Exanimate complexes appear to have a diff erent mode of mechanism of action compared with cisplatin which includes apoptotic and necrotic cell death. ere are indications that further investigations of these compounds may be very useful in overcoming the problems associated global cancer statistic. © 2016, University of Kragujevac, Faculty of Science. All rights reserved., Kompleski platine koriste se kao osnova za dizajn novih lekova. Oni su u širokoj upotrebi kao antitumorski agensi i do danas je oko 30 komplesa platine(II) i platine(IV) u nekoj od faza kliničkog ispitivanja. Posebno mesto u današnjim istaživanjima zauzimaju kompleksi metala sa edda ligandima. Uspešno je sintetisan veliki broj novih edda liganda i odgovarajućih kompleksa. Neki od ovih agensa pokazuju bolju aktivnost od zlatnog standarda, cisplatine. Pokazano je da postoji moguća veza između dužine ugljovodiničnog lanca estraske grupe liganda i citotoksičnog efekta. U većini slučajeva dužina lanca direktno korelira sa antitumorskom aktivnošću. Zabeležena je efikasnija citotoksicna aktivnost određenih kompleska platine sa edda ligandima na ćelijskim linijama tumora koji pokazuju odgovarajući stepen rezistencije na cisplatinu. Ispitivani komleksi imaju različit mehanizam dejstva od cisplatine, koji uključuje elemente nekrotične i programirane ćelijske smrti. Postoje nagoveštaji da dalja istraživanja ovih agensa mogu biti značajna za prevazilaženje globalnog problema sa kojim se svet danas suočava, a koji se odnosi na stalni porast osoba obolelih od karcinoma.",
journal = "Serbian Journal of Experimental and Clinical Research (ranije: Medicus)",
title = "Platinum complexes with edda ethylenediamine n, n diacetate ligands as potential anticancer agents [Kompleksi platine sa edda etilendiamin N, N’ diacetat ligandima kao potencijalni antitumorski agensi], Kompleksi platine sa edda (etilendiamin-N, N'-diacetat) ligandima kao potencijalni antitumorski agensi",
volume = "17",
number = "4",
pages = "285-295",
doi = "10.1515/SJECR-2016-0042"
}

Jurišević, M., Radosavljević, G., Arsenijević, A., Milovanović, M., Gajović, N., Đorđević, D. S., Milovanović, J., Stojanović, B., Ilić, A., Sabo, T.,& Kanjevac, T.. (2016). Platinum complexes with edda ethylenediamine n, n diacetate ligands as potential anticancer agents [Kompleksi platine sa edda etilendiamin N, N’ diacetat ligandima kao potencijalni antitumorski agensi]. in Serbian Journal of Experimental and Clinical Research (ranije: Medicus), 17(4), 285-295.
https://doi.org/10.1515/SJECR-2016-0042

Jurišević M, Radosavljević G, Arsenijević A, Milovanović M, Gajović N, Đorđević DS, Milovanović J, Stojanović B, Ilić A, Sabo T, Kanjevac T. Platinum complexes with edda ethylenediamine n, n diacetate ligands as potential anticancer agents [Kompleksi platine sa edda etilendiamin N, N’ diacetat ligandima kao potencijalni antitumorski agensi]. in Serbian Journal of Experimental and Clinical Research (ranije: Medicus). 2016;17(4):285-295.
doi:10.1515/SJECR-2016-0042 .

Jurišević, Milena, Radosavljević, Gordana, Arsenijević, Aleksandar, Milovanović, Marija, Gajović, Nevena, Đorđević, Dragana S., Milovanović, Jelena, Stojanović, Bojana, Ilić, Aleksandar, Sabo, Tibor, Kanjevac, Tatjana, "Platinum complexes with edda ethylenediamine n, n diacetate ligands as potential anticancer agents [Kompleksi platine sa edda etilendiamin N, N’ diacetat ligandima kao potencijalni antitumorski agensi]" in Serbian Journal of Experimental and Clinical Research (ranije: Medicus), 17, no. 4 (2016):285-295,
https://doi.org/10.1515/SJECR-2016-0042 . .