TY  - JOUR
AU  - Djurić, Aleksandra
AU  - Gojgić-Cvijović, Gordana D.
AU  - Jakovljević, Dragica M.
AU  - Kekez, Branka
AU  - Stefanović Kojić, Jovana R.
AU  - Mattinen, Maija-Liisa
AU  - Harju, Inka Elina
AU  - Vrvić, Miroslav M.
AU  - Beškoski, Vladimir
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2538
AB  - Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NMR) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with beta-configuration, where fructose units are linked by beta-2,6-glycosidic bonds, with beta-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan. (C) 2017 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan
VL  - 104
SP  - 311
EP  - 321
DO  - 10.1016/j.ijbiomac.2017.06.034
ER  - 

@article{
author = "Djurić, Aleksandra and Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica M. and Kekez, Branka and Stefanović Kojić, Jovana R. and Mattinen, Maija-Liisa and Harju, Inka Elina and Vrvić, Miroslav M. and Beškoski, Vladimir",
year = "2017",
abstract = "Various microorganisms isolated from polluted environments, such as Pseudomonas sp. and Micrococcus sp. can synthesize exopolysaccharides (EPSs) which are natural, non-toxic and biodegradable polymers. EPSs play a key role in protection of microbial cells under various external influences. For humans, these substances have potential use in many industries. EPSs can be applied as a flavor or a fragrance carrier, an emulsifier, a stabilizer, a prebiotic, an antioxidant or an antitumor agent. In this study, we characterized an environmental microorganism that produces EPS, optimized EPS production by this strain and characterized the EPS produced. Isolate CH-KOV3 was identified as Brachybacterium paraconglomeratum. The sucrose level in the growth medium greatly influenced EPS production, and the highest yield was when the microorganism was incubated in media with 500 g/L, of sucrose. The optimal temperature and pH were 28 degrees C and 7.0, respectively. The nuclear magnetic resonance (NMR) results and GC MS analysis confirmed that the residues were D-fructofuranosyl residues with beta-configuration, where fructose units are linked by beta-2,6-glycosidic bonds, with beta-2,1-linked branches. All these data indicate that the investigated EPS is a levan-type polysaccharide. Thus, it was concluded that Brachybacterium sp. CH-KOV3 could constitute a new source for production of the bioactive polysaccharide, levan. (C) 2017 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan",
volume = "104",
pages = "311-321",
doi = "10.1016/j.ijbiomac.2017.06.034"
}

Djurić, A., Gojgić-Cvijović, G. D., Jakovljević, D. M., Kekez, B., Stefanović Kojić, J. R., Mattinen, M., Harju, I. E., Vrvić, M. M.,& Beškoski, V.. (2017). Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan. in International Journal of Biological Macromolecules
Elsevier Science Bv, Amsterdam., 104, 311-321.
https://doi.org/10.1016/j.ijbiomac.2017.06.034

Djurić A, Gojgić-Cvijović GD, Jakovljević DM, Kekez B, Stefanović Kojić JR, Mattinen M, Harju IE, Vrvić MM, Beškoski V. Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan. in International Journal of Biological Macromolecules. 2017;104:311-321.
doi:10.1016/j.ijbiomac.2017.06.034 .

Djurić, Aleksandra, Gojgić-Cvijović, Gordana D., Jakovljević, Dragica M., Kekez, Branka, Stefanović Kojić, Jovana R., Mattinen, Maija-Liisa, Harju, Inka Elina, Vrvić, Miroslav M., Beškoski, Vladimir, "Brachybacterium sp CH-KOV3 isolated from an oil-polluted environment-a new producer of levan" in International Journal of Biological Macromolecules, 104 (2017):311-321,
https://doi.org/10.1016/j.ijbiomac.2017.06.034 . .

TY  - DATA
AU  - Djurić, Aleksandra
AU  - Gojgić-Cvijović, Gordana D.
AU  - Jakovljević, Dragica M.
AU  - Kekez, Branka
AU  - Stefanović Kojić, Jovana R.
AU  - Mattinen, Maija-Liisa
AU  - Harju, Inka Elina
AU  - Vrvić, Miroslav M.
AU  - Beškoski, Vladimir
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2965
PB  - Elsevier Science Bv, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Supplementary data for article: Djuric, A.; Gojgić-Cvijović, G. D.; Jakovljević, D. M.; Kekez, B.; Kojic, J. S.; Mattinen, M.-L.; Harju, I. E.; Vrvic, M. M.; Beškoski, V. Brachybacterium Sp CH-KOV3 Isolated from an Oil-Polluted Environment-a New Producer of Levan. International Journal of Biological Macromolecules 2017, 104, 311–321. https://doi.org/10.1016/j.ijbiomac.2017.06.034
UR  - https://hdl.handle.net/21.15107/rcub_cherry_2965
ER  - 

@misc{
author = "Djurić, Aleksandra and Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica M. and Kekez, Branka and Stefanović Kojić, Jovana R. and Mattinen, Maija-Liisa and Harju, Inka Elina and Vrvić, Miroslav M. and Beškoski, Vladimir",
year = "2017",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Supplementary data for article: Djuric, A.; Gojgić-Cvijović, G. D.; Jakovljević, D. M.; Kekez, B.; Kojic, J. S.; Mattinen, M.-L.; Harju, I. E.; Vrvic, M. M.; Beškoski, V. Brachybacterium Sp CH-KOV3 Isolated from an Oil-Polluted Environment-a New Producer of Levan. International Journal of Biological Macromolecules 2017, 104, 311–321. https://doi.org/10.1016/j.ijbiomac.2017.06.034",
url = "https://hdl.handle.net/21.15107/rcub_cherry_2965"
}

Djurić, A., Gojgić-Cvijović, G. D., Jakovljević, D. M., Kekez, B., Stefanović Kojić, J. R., Mattinen, M., Harju, I. E., Vrvić, M. M.,& Beškoski, V.. (2017). Supplementary data for article: Djuric, A.; Gojgić-Cvijović, G. D.; Jakovljević, D. M.; Kekez, B.; Kojic, J. S.; Mattinen, M.-L.; Harju, I. E.; Vrvic, M. M.; Beškoski, V. Brachybacterium Sp CH-KOV3 Isolated from an Oil-Polluted Environment-a New Producer of Levan. International Journal of Biological Macromolecules 2017, 104, 311–321. https://doi.org/10.1016/j.ijbiomac.2017.06.034. in International Journal of Biological Macromolecules
Elsevier Science Bv, Amsterdam..
https://hdl.handle.net/21.15107/rcub_cherry_2965

Djurić A, Gojgić-Cvijović GD, Jakovljević DM, Kekez B, Stefanović Kojić JR, Mattinen M, Harju IE, Vrvić MM, Beškoski V. Supplementary data for article: Djuric, A.; Gojgić-Cvijović, G. D.; Jakovljević, D. M.; Kekez, B.; Kojic, J. S.; Mattinen, M.-L.; Harju, I. E.; Vrvic, M. M.; Beškoski, V. Brachybacterium Sp CH-KOV3 Isolated from an Oil-Polluted Environment-a New Producer of Levan. International Journal of Biological Macromolecules 2017, 104, 311–321. https://doi.org/10.1016/j.ijbiomac.2017.06.034. in International Journal of Biological Macromolecules. 2017;.
https://hdl.handle.net/21.15107/rcub_cherry_2965 .

Djurić, Aleksandra, Gojgić-Cvijović, Gordana D., Jakovljević, Dragica M., Kekez, Branka, Stefanović Kojić, Jovana R., Mattinen, Maija-Liisa, Harju, Inka Elina, Vrvić, Miroslav M., Beškoski, Vladimir, "Supplementary data for article: Djuric, A.; Gojgić-Cvijović, G. D.; Jakovljević, D. M.; Kekez, B.; Kojic, J. S.; Mattinen, M.-L.; Harju, I. E.; Vrvic, M. M.; Beškoski, V. Brachybacterium Sp CH-KOV3 Isolated from an Oil-Polluted Environment-a New Producer of Levan. International Journal of Biological Macromolecules 2017, 104, 311–321. https://doi.org/10.1016/j.ijbiomac.2017.06.034" in International Journal of Biological Macromolecules (2017),
https://hdl.handle.net/21.15107/rcub_cherry_2965 .

TY  - JOUR
AU  - Avdalović, Jelena
AU  - Beškoski, Vladimir
AU  - Gojgić-Cvijović, Gordana D.
AU  - Mattinen, Maija-Liisa
AU  - Stojanović, Mirjana D.
AU  - Zildžović, Snežana
AU  - Vrvić, Miroslav M.
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1665
AB  - Procedures for solubilization of phosphorus from insoluble phosphates by acidophilic autotrophs and heterotrophs are considered to be research subjects of great importance in sustainable soil management, and could become, in the future, an alternative to current expensive methods of industrial fertilizer production. Furthermore, this biological production of fertilizer can be considered to be more environmentally friendly than current production methods. The object of this study was to investigate the possibility of phosphorus leaching from phosphate rock apatite using sulphuric acid generated from pyrite by iron-oxidizing Acidithiobacillus sp. B2, and to test the possible application of the mixture of these two minerals to produce a natural phosphate fertilizer. Iron-oxidizing Acidithiobacillus sp. B2 was isolated from copper sulphide mine wastewater (Lake Robule) in Bar, Serbia. The bacterium was identified by 16SrDNA oligonucleotide sequence and characterized using matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF MS). The leaching experiment was carried out in laboratory conditions at 28 degrees C for a period of four weeks. The results showed that up to 34.5% of the inorganic phosphorus was leached from the test mixture when in suspension with iron-oxidizing Acidithiobacillus sp. B2, while at the same time, the degree of leaching from a control suspension (without iron-oxidizing Acidithiobacillus sp. B2) was only up to 3.8%. Due to its low cost and environmental acceptance, treatment of soil with phosphate minerals in combination with pyrite and acidophilic iron- and sulphur-oxidizing bacteria could become an attractive and alternative way to improve the quality of alkaline soils.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Minerals Engineering
T1  - Microbial solubilization of phosphorus from phosphate rock by iron-oxidizing Acidithiobacillus sp B2
VL  - 72
SP  - 17
EP  - 22
DO  - 10.1016/j.mineng.2014.12.010
ER  - 

@article{
author = "Avdalović, Jelena and Beškoski, Vladimir and Gojgić-Cvijović, Gordana D. and Mattinen, Maija-Liisa and Stojanović, Mirjana D. and Zildžović, Snežana and Vrvić, Miroslav M.",
year = "2015",
abstract = "Procedures for solubilization of phosphorus from insoluble phosphates by acidophilic autotrophs and heterotrophs are considered to be research subjects of great importance in sustainable soil management, and could become, in the future, an alternative to current expensive methods of industrial fertilizer production. Furthermore, this biological production of fertilizer can be considered to be more environmentally friendly than current production methods. The object of this study was to investigate the possibility of phosphorus leaching from phosphate rock apatite using sulphuric acid generated from pyrite by iron-oxidizing Acidithiobacillus sp. B2, and to test the possible application of the mixture of these two minerals to produce a natural phosphate fertilizer. Iron-oxidizing Acidithiobacillus sp. B2 was isolated from copper sulphide mine wastewater (Lake Robule) in Bar, Serbia. The bacterium was identified by 16SrDNA oligonucleotide sequence and characterized using matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF MS). The leaching experiment was carried out in laboratory conditions at 28 degrees C for a period of four weeks. The results showed that up to 34.5% of the inorganic phosphorus was leached from the test mixture when in suspension with iron-oxidizing Acidithiobacillus sp. B2, while at the same time, the degree of leaching from a control suspension (without iron-oxidizing Acidithiobacillus sp. B2) was only up to 3.8%. Due to its low cost and environmental acceptance, treatment of soil with phosphate minerals in combination with pyrite and acidophilic iron- and sulphur-oxidizing bacteria could become an attractive and alternative way to improve the quality of alkaline soils.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Minerals Engineering",
title = "Microbial solubilization of phosphorus from phosphate rock by iron-oxidizing Acidithiobacillus sp B2",
volume = "72",
pages = "17-22",
doi = "10.1016/j.mineng.2014.12.010"
}

Avdalović, J., Beškoski, V., Gojgić-Cvijović, G. D., Mattinen, M., Stojanović, M. D., Zildžović, S.,& Vrvić, M. M.. (2015). Microbial solubilization of phosphorus from phosphate rock by iron-oxidizing Acidithiobacillus sp B2. in Minerals Engineering
Pergamon-Elsevier Science Ltd, Oxford., 72, 17-22.
https://doi.org/10.1016/j.mineng.2014.12.010

Avdalović J, Beškoski V, Gojgić-Cvijović GD, Mattinen M, Stojanović MD, Zildžović S, Vrvić MM. Microbial solubilization of phosphorus from phosphate rock by iron-oxidizing Acidithiobacillus sp B2. in Minerals Engineering. 2015;72:17-22.
doi:10.1016/j.mineng.2014.12.010 .

Avdalović, Jelena, Beškoski, Vladimir, Gojgić-Cvijović, Gordana D., Mattinen, Maija-Liisa, Stojanović, Mirjana D., Zildžović, Snežana, Vrvić, Miroslav M., "Microbial solubilization of phosphorus from phosphate rock by iron-oxidizing Acidithiobacillus sp B2" in Minerals Engineering, 72 (2015):17-22,
https://doi.org/10.1016/j.mineng.2014.12.010 . .