Krstić-Ristivojević, Maja

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Monitoring of in vitro bioavailability and uptake of glycosylated food allergens using cell-based models

Krstić-Ristivojević, Maja

(Универзитет у Београду, Хемијски факултет, 2020)

TY  - BOOK
AU  - Krstić-Ristivojević, Maja
PY  - 2020
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7891
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:23228/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=30780681
UR  - https://nardus.mpn.gov.rs/handle/123456789/17803
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4362
AB  - extensive engagement of researchers in the elucidation of the mechanismsunderlying processes of food digestion, allergen transport, and uptake by the immunecells and its effector immune responses. The development of the in vitro assays andcell-based models has allowed bridging the problem of food allergy research and therespect of the ethical norms in used research procedures.The red meat allergy is a novel type of food allergy characterized by theproduction of an IgE antibody against the carbohydrate galactose-α-1,3-galactose (α-Gal). Glycoproteins from non-primate mammals are rich with an α-Gal as posttranslationalmodification. Also, red meat allergy is characterized by the delayed onsetof symptoms which may be related to the mechanism and the fate of α-Gal carryingproteins in the human gastrointestinal tract. Furthermore, the uptake, processing, andmechanisms of presentation of α-Gal by the immune cells are still unknown. Thereforethis doctoral dissertation aimed to investigate how protein glycosylation by α-Galaffects their susceptibility to gastric digestion, does α-Gal conjugated to proteins affectstheir transport through the Caco-2 cell monolayer, which mimics the gastrointestinallayer, and to examine the influence of α-Gal epitopes on the protein surface on theiruptake and processing by immature monocyte-derived dendritic cells (iMDDCs). Thestudy revealed that the presence of the α-Gal glycosylation on protein surface had animpact on their susceptibility to gastric digestion and the digestion pattern of theobtained protein fragments upon pepsinolysis. Prolonged survival, up to 2h of digestion,was characteristic of the large proteins fragments bearing the α-Gal epitope.Importantly, transport through the Caco-2 monolayer of proteins conjugated to α-Galwas hampered in comparison to unconjugated proteins. Furthermore, differentialcentrifugation of Caco-2 cell lysates upon transport experiments revealed that α-Galcould be detected on the intact protein in the endosomal fraction of the cells...
AB  - Alergije na hranu su rastući problem u ljudskoj populaciji širom sveta irešavanje ovog problema zahteva opsežno angažovanje istraživača u rasvetljavanjumehanizama uključenih u procese varenja hrane, transporta alergena i njihovog unosaod strane imunih ćelija odgovornih za efektorske mehanizme imunoloških odgovora.Ovo je nezamislivo bez razvoja in vitro testova i ćelijskih modela koji premošćujuproblem istraživanja alergija na hranu uz poštovanja etičkih normi u korišćenimistraživačkim metodama.Novu vrstu alergije na hranu, alergiju na crveno meso, karakteriše sintezaimunoglobulina E kao odgovor na prisustvo šećera galaktoza-α-1,3-galaktoza (α-Gal),koji je prisutan na površini glikoproteina primata. Takođe, alergiju na crveno mesokarakteriše odložena pojava simptoma što može biti rezultat promena u mehanizmuobrade proteina koji nose α-Gal u gastrointestinalnom traktu čoveka. Dalje, unos,obrada i mehanizmi prezentacije α-Gal šećera od strane imunih ćelija još uvek nisupoznati. Stoga ciljevi ove doktorske disertacije su ispitivanje kako α-Gal glikozilacijaproteina utiče na njihovu digestiju od strane pepsina, da li α-Gal glikozilacija proteinautiče na njihov transport kroz monosloj Caco-2 ćelija, koji oponaša gastrointestinalniepitel, kao i ispitivanje uticaja α-Gal glikozilacije na površini proteina na njihov unos iobradu od strane nezrelih dendritičnih ćelijama kultivisanih iz monocita (iMDDC). Izdobijenih rezultata moze se zaključiti da prisustvo α-Gal glikozilacije na površiniproteina utiče na njihovu podložnost na digestiju a najviše na obrazac dobijenihfragmenata proteina nakon pepsinolize. Veliki fragmenti proteina koji nose α-Galprisutni su čak i do 2 sata digestije. Takođe, važno je istaći da je transport α-Galglikozilovanih proteina kroz Caco-2 monosloj otežan u poređenju sa neglikozilovanimproteinima. Dalje, diferencijalnim centrifugiranjem lizata aco-2 ćelija nakontranscitoze pokazalo je da je α-Gal prisutan na intaktnim proteinima u endozomalnimfrakcijama ćelija...
PB  - Универзитет у Београду, Хемијски факултет
T2  - Универзитет у Београду
T1  - Monitoring of in vitro bioavailability and uptake of glycosylated food allergens using cell-based models
ER  - 
@phdthesis{
author = "Krstić-Ristivojević, Maja",
year = "2020",
url = "http://eteze.bg.ac.rs/application/showtheses?thesesId=7891, https://fedorabg.bg.ac.rs/fedora/get/o:23228/bdef:Content/download, http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=30780681, https://nardus.mpn.gov.rs/handle/123456789/17803, http://cherry.chem.bg.ac.rs/handle/123456789/4362",
abstract = "extensive engagement of researchers in the elucidation of the mechanismsunderlying processes of food digestion, allergen transport, and uptake by the immunecells and its effector immune responses. The development of the in vitro assays andcell-based models has allowed bridging the problem of food allergy research and therespect of the ethical norms in used research procedures.The red meat allergy is a novel type of food allergy characterized by theproduction of an IgE antibody against the carbohydrate galactose-α-1,3-galactose (α-Gal). Glycoproteins from non-primate mammals are rich with an α-Gal as posttranslationalmodification. Also, red meat allergy is characterized by the delayed onsetof symptoms which may be related to the mechanism and the fate of α-Gal carryingproteins in the human gastrointestinal tract. Furthermore, the uptake, processing, andmechanisms of presentation of α-Gal by the immune cells are still unknown. Thereforethis doctoral dissertation aimed to investigate how protein glycosylation by α-Galaffects their susceptibility to gastric digestion, does α-Gal conjugated to proteins affectstheir transport through the Caco-2 cell monolayer, which mimics the gastrointestinallayer, and to examine the influence of α-Gal epitopes on the protein surface on theiruptake and processing by immature monocyte-derived dendritic cells (iMDDCs). Thestudy revealed that the presence of the α-Gal glycosylation on protein surface had animpact on their susceptibility to gastric digestion and the digestion pattern of theobtained protein fragments upon pepsinolysis. Prolonged survival, up to 2h of digestion,was characteristic of the large proteins fragments bearing the α-Gal epitope.Importantly, transport through the Caco-2 monolayer of proteins conjugated to α-Galwas hampered in comparison to unconjugated proteins. Furthermore, differentialcentrifugation of Caco-2 cell lysates upon transport experiments revealed that α-Galcould be detected on the intact protein in the endosomal fraction of the cells..., Alergije na hranu su rastući problem u ljudskoj populaciji širom sveta irešavanje ovog problema zahteva opsežno angažovanje istraživača u rasvetljavanjumehanizama uključenih u procese varenja hrane, transporta alergena i njihovog unosaod strane imunih ćelija odgovornih za efektorske mehanizme imunoloških odgovora.Ovo je nezamislivo bez razvoja in vitro testova i ćelijskih modela koji premošćujuproblem istraživanja alergija na hranu uz poštovanja etičkih normi u korišćenimistraživačkim metodama.Novu vrstu alergije na hranu, alergiju na crveno meso, karakteriše sintezaimunoglobulina E kao odgovor na prisustvo šećera galaktoza-α-1,3-galaktoza (α-Gal),koji je prisutan na površini glikoproteina primata. Takođe, alergiju na crveno mesokarakteriše odložena pojava simptoma što može biti rezultat promena u mehanizmuobrade proteina koji nose α-Gal u gastrointestinalnom traktu čoveka. Dalje, unos,obrada i mehanizmi prezentacije α-Gal šećera od strane imunih ćelija još uvek nisupoznati. Stoga ciljevi ove doktorske disertacije su ispitivanje kako α-Gal glikozilacijaproteina utiče na njihovu digestiju od strane pepsina, da li α-Gal glikozilacija proteinautiče na njihov transport kroz monosloj Caco-2 ćelija, koji oponaša gastrointestinalniepitel, kao i ispitivanje uticaja α-Gal glikozilacije na površini proteina na njihov unos iobradu od strane nezrelih dendritičnih ćelijama kultivisanih iz monocita (iMDDC). Izdobijenih rezultata moze se zaključiti da prisustvo α-Gal glikozilacije na površiniproteina utiče na njihovu podložnost na digestiju a najviše na obrazac dobijenihfragmenata proteina nakon pepsinolize. Veliki fragmenti proteina koji nose α-Galprisutni su čak i do 2 sata digestije. Takođe, važno je istaći da je transport α-Galglikozilovanih proteina kroz Caco-2 monosloj otežan u poređenju sa neglikozilovanimproteinima. Dalje, diferencijalnim centrifugiranjem lizata aco-2 ćelija nakontranscitoze pokazalo je da je α-Gal prisutan na intaktnim proteinima u endozomalnimfrakcijama ćelija...",
publisher = "Универзитет у Београду, Хемијски факултет",
journal = "Универзитет у Београду",
title = "Monitoring of in vitro bioavailability and uptake of glycosylated food allergens using cell-based models"
}
Krstić-Ristivojević, M. (2020). Monitoring of in vitro bioavailability and uptake of glycosylated food allergens using cell-based models.
Универзитет у Београду
Универзитет у Београду, Хемијски факултет..
Krstić-Ristivojević M. Monitoring of in vitro bioavailability and uptake of glycosylated food allergens using cell-based models. Универзитет у Београду. 2020;
Krstić-Ristivojević Maja, "Monitoring of in vitro bioavailability and uptake of glycosylated food allergens using cell-based models" Универзитет у Београду (2020)

Fatty acids composition of the most common bivalves in Korean diet

Krstić-Ristivojević, Maja; Jovanović, Vesna B.; Ristivojević, Petar; Ćirković-Veličković, Tanja

(Portugal : Sociedade Portuguesa de Química, 2019)

TY  - CONF
AU  - Krstić-Ristivojević, Maja
AU  - Jovanović, Vesna B.
AU  - Ristivojević, Petar
AU  - Ćirković-Veličković, Tanja
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3777
AB  - Consumption of bivalve molluscs, such as oysters, mussels, clams and scallops, makes a significant part of the daily Korean diet. Bivalves provide high quality proteins with all the dietary-essential amino acids, lipids, vitamins, minerals and other bioactive nutrients, which offer a variety of health benefits to the consumer [1]. This food contains less than 5 percent of total fat, so it is considered a low-fat food. Beside the amount of total fat, the proportions of saturated, monounsaturated and polyunsaturated fatty acids (FA) (S, M and P, respectively), as well as ratio of n-3 (ω-3) and n-6 (ω-6) P in food are very important for the health diet [2].
Fourteen species of bivalves Anadara broughtonii (AB), Ruditapes philippinarum (Manila clam (RP)), Tegillarca granosa (TG), Pecten yessoensis (Yesso scallop (YS), Argopecten spp. (small scallop (SS), Chlamys farreri farreri (CF), Cyclina sinensis (CS), Leukoma jedoensis (LJ), Mytilus califorianus (MCa) Mytilus galloprovancialis (MG), Maretrix lusoria (ML), Mactra quadrangularis (MQ), Sinovacula constricta (SC) and Crassostrea gigas (Pacific oyster (PC)) were bought in two fish markets in Incheon, Korea, in order to determine FA composition using GC/EI-MS of fatty acid methyl esters (FAME). The FAME were identified by comparing their retention times with those of the FAME standards or by comparing their mass spectra with those stored in the NIST Mass Spectral Library.
In the bivalve samples, 43 different FA were identified, of which 10 were S, 12 M and 13 P, other FA were 7 methyl-FA and 1 hydroxyl-FA. The P/S ratio and ω-6/ω-3 P ratio are the most significant markers of lipid composition in a healthy diet and both should be close to 1 [3]. Among analysed species, only YS and SS have P/S ratio close to 1 (1,20 and 1.16, respectively), while other species have value between 0.07 and 0.73. The obtained values for ω-6/ω-3 P ratio were from 0.008 to 0.55, which indicates that bivalve molluscs are the valuable source of ω-3 P (EPA and DHA). These ω-3 P play important roles in growth, development, and maintenance of health.
PB  - Portugal : Sociedade Portuguesa de Química
C3  - Book of Abstracts of the XX EuroFoodChem Congress
T1  - Fatty acids composition of the most common bivalves in Korean diet
ER  - 
@conference{
author = "Krstić-Ristivojević, Maja and Jovanović, Vesna B. and Ristivojević, Petar and Ćirković-Veličković, Tanja",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3777",
abstract = "Consumption of bivalve molluscs, such as oysters, mussels, clams and scallops, makes a significant part of the daily Korean diet. Bivalves provide high quality proteins with all the dietary-essential amino acids, lipids, vitamins, minerals and other bioactive nutrients, which offer a variety of health benefits to the consumer [1]. This food contains less than 5 percent of total fat, so it is considered a low-fat food. Beside the amount of total fat, the proportions of saturated, monounsaturated and polyunsaturated fatty acids (FA) (S, M and P, respectively), as well as ratio of n-3 (ω-3) and n-6 (ω-6) P in food are very important for the health diet [2].
Fourteen species of bivalves Anadara broughtonii (AB), Ruditapes philippinarum (Manila clam (RP)), Tegillarca granosa (TG), Pecten yessoensis (Yesso scallop (YS), Argopecten spp. (small scallop (SS), Chlamys farreri farreri (CF), Cyclina sinensis (CS), Leukoma jedoensis (LJ), Mytilus califorianus (MCa) Mytilus galloprovancialis (MG), Maretrix lusoria (ML), Mactra quadrangularis (MQ), Sinovacula constricta (SC) and Crassostrea gigas (Pacific oyster (PC)) were bought in two fish markets in Incheon, Korea, in order to determine FA composition using GC/EI-MS of fatty acid methyl esters (FAME). The FAME were identified by comparing their retention times with those of the FAME standards or by comparing their mass spectra with those stored in the NIST Mass Spectral Library.
In the bivalve samples, 43 different FA were identified, of which 10 were S, 12 M and 13 P, other FA were 7 methyl-FA and 1 hydroxyl-FA. The P/S ratio and ω-6/ω-3 P ratio are the most significant markers of lipid composition in a healthy diet and both should be close to 1 [3]. Among analysed species, only YS and SS have P/S ratio close to 1 (1,20 and 1.16, respectively), while other species have value between 0.07 and 0.73. The obtained values for ω-6/ω-3 P ratio were from 0.008 to 0.55, which indicates that bivalve molluscs are the valuable source of ω-3 P (EPA and DHA). These ω-3 P play important roles in growth, development, and maintenance of health.",
publisher = "Portugal : Sociedade Portuguesa de Química",
journal = "Book of Abstracts of the XX EuroFoodChem Congress",
title = "Fatty acids composition of the most common bivalves in Korean diet"
}
Krstić-Ristivojević, M., Jovanović, V. B., Ristivojević, P.,& Ćirković-Veličković, T. (2019). Fatty acids composition of the most common bivalves in Korean diet.
Book of Abstracts of the XX EuroFoodChem Congress
Portugal : Sociedade Portuguesa de Química..
Krstić-Ristivojević M, Jovanović VB, Ristivojević P, Ćirković-Veličković T. Fatty acids composition of the most common bivalves in Korean diet. Book of Abstracts of the XX EuroFoodChem Congress. 2019;
Krstić-Ristivojević Maja, Jovanović Vesna B., Ristivojević Petar, Ćirković-Veličković Tanja, "Fatty acids composition of the most common bivalves in Korean diet" Book of Abstracts of the XX EuroFoodChem Congress (2019)

alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells

Krstić-Ristivojević, Maja; Grundström, Jeanette; Tran, Thi Anh Thu; Apostolović, Danijela; Radoi, Vlad; Starkhammar, Maria; Vukojević, Vesna; Ćirković-Veličković, Tanja; Hamsten, Carl; van Hage, Marianne

(Nature Publishing Group, London, 2018)

TY  - JOUR
AU  - Krstić-Ristivojević, Maja
AU  - Grundström, Jeanette
AU  - Tran, Thi Anh Thu
AU  - Apostolović, Danijela
AU  - Radoi, Vlad
AU  - Starkhammar, Maria
AU  - Vukojević, Vesna
AU  - Ćirković-Veličković, Tanja
AU  - Hamsten, Carl
AU  - van Hage, Marianne
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2207
AB  - Red meat allergy is characterized by an IgE response against the carbohydrate galactose-alpha-1,3-galactose (alpha-Gal), which is abundantly expressed on glycoproteins from non-primate mammals. The mechanisms of how alpha-Gal is processed and presented to the immune system to initiate an allergic reaction are still unknown. The aim of this study was to reveal whether the presence of alpha-Gal epitopes on the protein surface influence antigen uptake and processing in immature monocyte-derived dendritic cells (iMDDCs). Immature MDDCs were prepared from healthy blood donors and red meat allergic patients. We found an increased internalization of alpha-Gal carrying proteins over time in iMDDCs by flow cytometric analysis, which was independent of the donor allergic status. The uptake of alpha-Gal carrying proteins was significantly higher than the uptake of non-alpha-Gal carrying proteins. Confocal microscopy revealed alpha-Gal carrying proteins scattered around the cytoplasm in most iMDDCs while detection of proteins not carrying alpha-Gal was negligible. Fluorescent detection of protein on SDS-PAGE showed that degradation of alpha-Gal carrying proteins was slower than degradation of non-alpha-Gal carrying proteins. Thus, the presence of alpha-Gal on the protein surface affects both uptake and degradation of the protein, and the results add new knowledge of alpha-Gal as a clinically relevant food allergen.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells
VL  - 8
DO  - 10.1038/s41598-018-30887-8
ER  - 
@article{
author = "Krstić-Ristivojević, Maja and Grundström, Jeanette and Tran, Thi Anh Thu and Apostolović, Danijela and Radoi, Vlad and Starkhammar, Maria and Vukojević, Vesna and Ćirković-Veličković, Tanja and Hamsten, Carl and van Hage, Marianne",
year = "2018",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2207",
abstract = "Red meat allergy is characterized by an IgE response against the carbohydrate galactose-alpha-1,3-galactose (alpha-Gal), which is abundantly expressed on glycoproteins from non-primate mammals. The mechanisms of how alpha-Gal is processed and presented to the immune system to initiate an allergic reaction are still unknown. The aim of this study was to reveal whether the presence of alpha-Gal epitopes on the protein surface influence antigen uptake and processing in immature monocyte-derived dendritic cells (iMDDCs). Immature MDDCs were prepared from healthy blood donors and red meat allergic patients. We found an increased internalization of alpha-Gal carrying proteins over time in iMDDCs by flow cytometric analysis, which was independent of the donor allergic status. The uptake of alpha-Gal carrying proteins was significantly higher than the uptake of non-alpha-Gal carrying proteins. Confocal microscopy revealed alpha-Gal carrying proteins scattered around the cytoplasm in most iMDDCs while detection of proteins not carrying alpha-Gal was negligible. Fluorescent detection of protein on SDS-PAGE showed that degradation of alpha-Gal carrying proteins was slower than degradation of non-alpha-Gal carrying proteins. Thus, the presence of alpha-Gal on the protein surface affects both uptake and degradation of the protein, and the results add new knowledge of alpha-Gal as a clinically relevant food allergen.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells",
volume = "8",
doi = "10.1038/s41598-018-30887-8"
}
Krstić-Ristivojević, M., Grundström, J., Tran, T. A. T., Apostolović, D., Radoi, V., Starkhammar, M., Vukojević, V., Ćirković-Veličković, T., Hamsten, C.,& van Hage, M. (2018). alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells.
Scientific Reports
Nature Publishing Group, London., 8.
https://doi.org/10.1038/s41598-018-30887-8
Krstić-Ristivojević M, Grundström J, Tran TAT, Apostolović D, Radoi V, Starkhammar M, Vukojević V, Ćirković-Veličković T, Hamsten C, van Hage M. alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells. Scientific Reports. 2018;8
Krstić-Ristivojević Maja, Grundström Jeanette, Tran Thi Anh Thu, Apostolović Danijela, Radoi Vlad, Starkhammar Maria, Vukojević Vesna, Ćirković-Veličković Tanja, Hamsten Carl, van Hage Marianne, "alpha-Gal on the protein surface affects uptake and degradation in immature monocyte derived dendritic cells" Scientific Reports, 8 (2018),
https://doi.org/10.1038/s41598-018-30887-8 .
4
7
5
6

Supplementary data for the article: Ristivojević, M. K.; Grundström, J.; Tran, T. A. T.; Apostolovic, D.; Radoi, V.; Starkhammar, M.; Vukojević, V.; Ćirković Veličković, T.; Hamsten, C.; van Hage, M. α-Gal on the Protein Surface Affects Uptake and Degradation in Immature Monocyte Derived Dendritic Cells. Scientific Reports 2018, 8 (1). https://doi.org/10.1038/s41598-018-30887-8

Krstić-Ristivojević, Maja; Grundström, Jeanette; Tran, Thi Anh Thu; Apostolović, Danijela; Radoi, Vlad; Starkhammar, Maria; Vukojević, Vesna; Ćirković-Veličković, Tanja; Hamsten, Carl; van Hage, Marianne

(Nature Publishing Group, London, 2018)

TY  - BOOK
AU  - Krstić-Ristivojević, Maja
AU  - Grundström, Jeanette
AU  - Tran, Thi Anh Thu
AU  - Apostolović, Danijela
AU  - Radoi, Vlad
AU  - Starkhammar, Maria
AU  - Vukojević, Vesna
AU  - Ćirković-Veličković, Tanja
AU  - Hamsten, Carl
AU  - van Hage, Marianne
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3073
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Supplementary data for the article: Ristivojević, M. K.; Grundström, J.; Tran, T. A. T.; Apostolovic, D.; Radoi, V.; Starkhammar, M.; Vukojević, V.; Ćirković Veličković, T.; Hamsten, C.; van Hage, M. α-Gal on the Protein Surface Affects Uptake and Degradation in Immature Monocyte Derived Dendritic Cells. Scientific Reports 2018, 8 (1). https://doi.org/10.1038/s41598-018-30887-8
ER  - 
@book{
author = "Krstić-Ristivojević, Maja and Grundström, Jeanette and Tran, Thi Anh Thu and Apostolović, Danijela and Radoi, Vlad and Starkhammar, Maria and Vukojević, Vesna and Ćirković-Veličković, Tanja and Hamsten, Carl and van Hage, Marianne",
year = "2018",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3073",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Supplementary data for the article: Ristivojević, M. K.; Grundström, J.; Tran, T. A. T.; Apostolovic, D.; Radoi, V.; Starkhammar, M.; Vukojević, V.; Ćirković Veličković, T.; Hamsten, C.; van Hage, M. α-Gal on the Protein Surface Affects Uptake and Degradation in Immature Monocyte Derived Dendritic Cells. Scientific Reports 2018, 8 (1). https://doi.org/10.1038/s41598-018-30887-8"
}
Krstić-Ristivojević, M., Grundström, J., Tran, T. A. T., Apostolović, D., Radoi, V., Starkhammar, M., Vukojević, V., Ćirković-Veličković, T., Hamsten, C.,& van Hage, M. (2018). Supplementary data for the article: Ristivojević, M. K.; Grundström, J.; Tran, T. A. T.; Apostolovic, D.; Radoi, V.; Starkhammar, M.; Vukojević, V.; Ćirković Veličković, T.; Hamsten, C.; van Hage, M. α-Gal on the Protein Surface Affects Uptake and Degradation in Immature Monocyte Derived Dendritic Cells. Scientific Reports 2018, 8 (1). https://doi.org/10.1038/s41598-018-30887-8.
Scientific Reports
Nature Publishing Group, London..
Krstić-Ristivojević M, Grundström J, Tran TAT, Apostolović D, Radoi V, Starkhammar M, Vukojević V, Ćirković-Veličković T, Hamsten C, van Hage M. Supplementary data for the article: Ristivojević, M. K.; Grundström, J.; Tran, T. A. T.; Apostolovic, D.; Radoi, V.; Starkhammar, M.; Vukojević, V.; Ćirković Veličković, T.; Hamsten, C.; van Hage, M. α-Gal on the Protein Surface Affects Uptake and Degradation in Immature Monocyte Derived Dendritic Cells. Scientific Reports 2018, 8 (1). https://doi.org/10.1038/s41598-018-30887-8. Scientific Reports. 2018;
Krstić-Ristivojević Maja, Grundström Jeanette, Tran Thi Anh Thu, Apostolović Danijela, Radoi Vlad, Starkhammar Maria, Vukojević Vesna, Ćirković-Veličković Tanja, Hamsten Carl, van Hage Marianne, "Supplementary data for the article: Ristivojević, M. K.; Grundström, J.; Tran, T. A. T.; Apostolovic, D.; Radoi, V.; Starkhammar, M.; Vukojević, V.; Ćirković Veličković, T.; Hamsten, C.; van Hage, M. α-Gal on the Protein Surface Affects Uptake and Degradation in Immature Monocyte Derived Dendritic Cells. Scientific Reports 2018, 8 (1). https://doi.org/10.1038/s41598-018-30887-8" Scientific Reports (2018)

Supplementary data for article: Apostolovic, D.; Krstic, M.; Mihailovic, J.; Starkhammar, M.; Cirkovic Velickovic, T.; Hamsten, C.; Van Hage, M. Peptidomics of an in Vitro Digested α-Gal Carrying Protein Revealed IgE-Reactive Peptides. Scientific Reports 2017, 7 (1). https://doi.org/10.1038/s41598-017-05355-4

Apostolović, Danijela; Krstić-Ristivojević, Maja; Mihailović-Vesić, Jelena; Starkhammar, Maria; Ćirković-Veličković, Tanja; Hamsten, Carl; van Hage, Marianne

(Nature Publishing Group, London, 2017)

TY  - BOOK
AU  - Apostolović, Danijela
AU  - Krstić-Ristivojević, Maja
AU  - Mihailović-Vesić, Jelena
AU  - Starkhammar, Maria
AU  - Ćirković-Veličković, Tanja
AU  - Hamsten, Carl
AU  - van Hage, Marianne
PY  - 2017
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3020
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Supplementary data for article: Apostolovic, D.; Krstic, M.; Mihailovic, J.; Starkhammar, M.; Cirkovic Velickovic, T.; Hamsten, C.; Van Hage, M. Peptidomics of an in Vitro Digested α-Gal Carrying Protein Revealed IgE-Reactive Peptides. Scientific Reports 2017, 7 (1). https://doi.org/10.1038/s41598-017-05355-4
VL  - 7
IS  - 1
ER  - 
@book{
author = "Apostolović, Danijela and Krstić-Ristivojević, Maja and Mihailović-Vesić, Jelena and Starkhammar, Maria and Ćirković-Veličković, Tanja and Hamsten, Carl and van Hage, Marianne",
year = "2017",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3020",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Supplementary data for article: Apostolovic, D.; Krstic, M.; Mihailovic, J.; Starkhammar, M.; Cirkovic Velickovic, T.; Hamsten, C.; Van Hage, M. Peptidomics of an in Vitro Digested α-Gal Carrying Protein Revealed IgE-Reactive Peptides. Scientific Reports 2017, 7 (1). https://doi.org/10.1038/s41598-017-05355-4",
volume = "7",
number = "1"
}
Apostolović, D., Krstić-Ristivojević, M., Mihailović-Vesić, J., Starkhammar, M., Ćirković-Veličković, T., Hamsten, C.,& van Hage, M. (2017). Supplementary data for article: Apostolovic, D.; Krstic, M.; Mihailovic, J.; Starkhammar, M.; Cirkovic Velickovic, T.; Hamsten, C.; Van Hage, M. Peptidomics of an in Vitro Digested α-Gal Carrying Protein Revealed IgE-Reactive Peptides. Scientific Reports 2017, 7 (1). https://doi.org/10.1038/s41598-017-05355-4.
Scientific Reports
Nature Publishing Group, London., 7(1).
Apostolović D, Krstić-Ristivojević M, Mihailović-Vesić J, Starkhammar M, Ćirković-Veličković T, Hamsten C, van Hage M. Supplementary data for article: Apostolovic, D.; Krstic, M.; Mihailovic, J.; Starkhammar, M.; Cirkovic Velickovic, T.; Hamsten, C.; Van Hage, M. Peptidomics of an in Vitro Digested α-Gal Carrying Protein Revealed IgE-Reactive Peptides. Scientific Reports 2017, 7 (1). https://doi.org/10.1038/s41598-017-05355-4. Scientific Reports. 2017;7(1)
Apostolović Danijela, Krstić-Ristivojević Maja, Mihailović-Vesić Jelena, Starkhammar Maria, Ćirković-Veličković Tanja, Hamsten Carl, van Hage Marianne, "Supplementary data for article: Apostolovic, D.; Krstic, M.; Mihailovic, J.; Starkhammar, M.; Cirkovic Velickovic, T.; Hamsten, C.; Van Hage, M. Peptidomics of an in Vitro Digested α-Gal Carrying Protein Revealed IgE-Reactive Peptides. Scientific Reports 2017, 7 (1). https://doi.org/10.1038/s41598-017-05355-4" Scientific Reports, 7, no. 1 (2017)

Peptidomics of an in vitro digested alpha-Gal carrying protein revealed IgE-reactive peptides

Apostolović, Danijela; Krstić-Ristivojević, Maja; Mihailović-Vesić, Jelena; Starkhammar, Maria; Ćirković-Veličković, Tanja; Hamsten, Carl; van Hage, Marianne

(Nature Publishing Group, London, 2017)

TY  - JOUR
AU  - Apostolović, Danijela
AU  - Krstić-Ristivojević, Maja
AU  - Mihailović-Vesić, Jelena
AU  - Starkhammar, Maria
AU  - Ćirković-Veličković, Tanja
AU  - Hamsten, Carl
AU  - van Hage, Marianne
PY  - 2017
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2486
AB  - The mammalian carbohydrate galactose-alpha 1,3-galactose (alpha-Gal) causes a novel form of food allergy, red meat allergy, where patients experience severe allergic reactions several hours after red meat consumption. Here we explored gastric digestion of alpha-Gal glycoproteins using an in vitro model. Bovine thyroglobulin (BTG), a typical alpha-Gal carrying glycoprotein, was digested with pepsin. The resulting peptides were characterised by SDS PAGE, immunoblot and ImmunoCAP using sera from 20 red meat allergic patients. During pepsinolysis of BTG, a wide range of peptide bands was observed of which 14 to 17 kDa peptides remained stable throughout the gastric phase. The presence of the alpha-Gal epitope on the obtained peptides was demonstrated by an anti-alpha-Gal antibody and IgE from red meat allergic patients. The alpha-Gal digests were able to inhibit up to 86% of IgE reactivity to BTG. Importantly, basophil activation test demonstrated that the allergenic activity of BTG was retained after digestion in all four tested patients. Mass spectrometry-based peptidomics revealed that these peptides represent mostly internal and C-terminal parts of the protein, where the most potent IgE-binding alpha-Gal residues were identified at Asn(1756), Asn(1850) and Asn(2231). Thus allergenic a-Gal epitopes are stable to pepsinolysis, reinforcing their role as clinically relevant food allergens.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Peptidomics of an in vitro digested alpha-Gal carrying protein revealed IgE-reactive peptides
VL  - 7
DO  - 10.1038/s41598-017-05355-4
ER  - 
@article{
author = "Apostolović, Danijela and Krstić-Ristivojević, Maja and Mihailović-Vesić, Jelena and Starkhammar, Maria and Ćirković-Veličković, Tanja and Hamsten, Carl and van Hage, Marianne",
year = "2017",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2486",
abstract = "The mammalian carbohydrate galactose-alpha 1,3-galactose (alpha-Gal) causes a novel form of food allergy, red meat allergy, where patients experience severe allergic reactions several hours after red meat consumption. Here we explored gastric digestion of alpha-Gal glycoproteins using an in vitro model. Bovine thyroglobulin (BTG), a typical alpha-Gal carrying glycoprotein, was digested with pepsin. The resulting peptides were characterised by SDS PAGE, immunoblot and ImmunoCAP using sera from 20 red meat allergic patients. During pepsinolysis of BTG, a wide range of peptide bands was observed of which 14 to 17 kDa peptides remained stable throughout the gastric phase. The presence of the alpha-Gal epitope on the obtained peptides was demonstrated by an anti-alpha-Gal antibody and IgE from red meat allergic patients. The alpha-Gal digests were able to inhibit up to 86% of IgE reactivity to BTG. Importantly, basophil activation test demonstrated that the allergenic activity of BTG was retained after digestion in all four tested patients. Mass spectrometry-based peptidomics revealed that these peptides represent mostly internal and C-terminal parts of the protein, where the most potent IgE-binding alpha-Gal residues were identified at Asn(1756), Asn(1850) and Asn(2231). Thus allergenic a-Gal epitopes are stable to pepsinolysis, reinforcing their role as clinically relevant food allergens.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Peptidomics of an in vitro digested alpha-Gal carrying protein revealed IgE-reactive peptides",
volume = "7",
doi = "10.1038/s41598-017-05355-4"
}
Apostolović, D., Krstić-Ristivojević, M., Mihailović-Vesić, J., Starkhammar, M., Ćirković-Veličković, T., Hamsten, C.,& van Hage, M. (2017). Peptidomics of an in vitro digested alpha-Gal carrying protein revealed IgE-reactive peptides.
Scientific Reports
Nature Publishing Group, London., 7.
https://doi.org/10.1038/s41598-017-05355-4
Apostolović D, Krstić-Ristivojević M, Mihailović-Vesić J, Starkhammar M, Ćirković-Veličković T, Hamsten C, van Hage M. Peptidomics of an in vitro digested alpha-Gal carrying protein revealed IgE-reactive peptides. Scientific Reports. 2017;7
Apostolović Danijela, Krstić-Ristivojević Maja, Mihailović-Vesić Jelena, Starkhammar Maria, Ćirković-Veličković Tanja, Hamsten Carl, van Hage Marianne, "Peptidomics of an in vitro digested alpha-Gal carrying protein revealed IgE-reactive peptides" Scientific Reports, 7 (2017),
https://doi.org/10.1038/s41598-017-05355-4 .
9
11
11

Alpha-gal epitope on protein surface affects uptake and degradation in immature monocyte-derived dendritic cells

Krstić-Ristivojević, Maja; Apostolović, Danijela; Tran, T.; Grundström, Jeanette; Radoi, Vlad; Vukojević, Vesna; Ćirković-Veličković, Tanja; Hamsten, Carl; van Hage, Marianne

(Wiley, Hoboken, 2017)

TY  - CONF
AU  - Krstić-Ristivojević, Maja
AU  - Apostolović, Danijela
AU  - Tran, T.
AU  - Grundström, Jeanette
AU  - Radoi, Vlad
AU  - Vukojević, Vesna
AU  - Ćirković-Veličković, Tanja
AU  - Hamsten, Carl
AU  - van Hage, Marianne
PY  - 2017
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2516
PB  - Wiley, Hoboken
C3  - Allergy
T1  - Alpha-gal epitope on protein surface affects uptake and degradation in immature monocyte-derived dendritic cells
VL  - 72
SP  - 796
EP  - 796
ER  - 
@conference{
author = "Krstić-Ristivojević, Maja and Apostolović, Danijela and Tran, T. and Grundström, Jeanette and Radoi, Vlad and Vukojević, Vesna and Ćirković-Veličković, Tanja and Hamsten, Carl and van Hage, Marianne",
year = "2017",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2516",
publisher = "Wiley, Hoboken",
journal = "Allergy",
title = "Alpha-gal epitope on protein surface affects uptake and degradation in immature monocyte-derived dendritic cells",
volume = "72",
pages = "796-796"
}
Krstić-Ristivojević, M., Apostolović, D., Tran, T., Grundström, J., Radoi, V., Vukojević, V., Ćirković-Veličković, T., Hamsten, C.,& van Hage, M. (2017). Alpha-gal epitope on protein surface affects uptake and degradation in immature monocyte-derived dendritic cells.
Allergy
Wiley, Hoboken., 72, 796-796.
Krstić-Ristivojević M, Apostolović D, Tran T, Grundström J, Radoi V, Vukojević V, Ćirković-Veličković T, Hamsten C, van Hage M. Alpha-gal epitope on protein surface affects uptake and degradation in immature monocyte-derived dendritic cells. Allergy. 2017;72:796-796
Krstić-Ristivojević Maja, Apostolović Danijela, Tran T., Grundström Jeanette, Radoi Vlad, Vukojević Vesna, Ćirković-Veličković Tanja, Hamsten Carl, van Hage Marianne, "Alpha-gal epitope on protein surface affects uptake and degradation in immature monocyte-derived dendritic cells" Allergy, 72 (2017):796-796

Supplementary data for the article: Mihajlovic, L.; Radosavljevic, J.; Nordlund, E.; Krstic, M.; Bohn, T.; Smit, J.; Buchert, J.; Cirkovic Velickovic, T. Peanut Protein Structure, Polyphenol Content and Immune Response to Peanut Proteins: In Vivo Are Modulated by Laccase. Food and Function 2016, 7 (5), 2357–2366. https://doi.org/10.1039/c5fo01325a

Mihajlović-Lalić, Ljiljana; Radosavljević, Jelena; Nordlund, Emilia; Krstić-Ristivojević, Maja; Bohn, Torsten; Smit, Joost; Buchert, Johanna; Ćirković-Veličković, Tanja

(Royal Soc Chemistry, Cambridge, 2016)

TY  - BOOK
AU  - Mihajlović-Lalić, Ljiljana
AU  - Radosavljević, Jelena
AU  - Nordlund, Emilia
AU  - Krstić-Ristivojević, Maja
AU  - Bohn, Torsten
AU  - Smit, Joost
AU  - Buchert, Johanna
AU  - Ćirković-Veličković, Tanja
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3591
PB  - Royal Soc Chemistry, Cambridge
T2  - Food and Function
T1  - Supplementary data for the article: Mihajlovic, L.; Radosavljevic, J.; Nordlund, E.; Krstic, M.; Bohn, T.; Smit, J.; Buchert, J.; Cirkovic Velickovic, T. Peanut Protein Structure, Polyphenol Content and Immune Response to Peanut Proteins: In Vivo Are Modulated by Laccase. Food and Function 2016, 7 (5), 2357–2366. https://doi.org/10.1039/c5fo01325a
ER  - 
@book{
author = "Mihajlović-Lalić, Ljiljana and Radosavljević, Jelena and Nordlund, Emilia and Krstić-Ristivojević, Maja and Bohn, Torsten and Smit, Joost and Buchert, Johanna and Ćirković-Veličković, Tanja",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3591",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food and Function",
title = "Supplementary data for the article: Mihajlovic, L.; Radosavljevic, J.; Nordlund, E.; Krstic, M.; Bohn, T.; Smit, J.; Buchert, J.; Cirkovic Velickovic, T. Peanut Protein Structure, Polyphenol Content and Immune Response to Peanut Proteins: In Vivo Are Modulated by Laccase. Food and Function 2016, 7 (5), 2357–2366. https://doi.org/10.1039/c5fo01325a"
}
Mihajlović-Lalić, L., Radosavljević, J., Nordlund, E., Krstić-Ristivojević, M., Bohn, T., Smit, J., Buchert, J.,& Ćirković-Veličković, T. (2016). Supplementary data for the article: Mihajlovic, L.; Radosavljevic, J.; Nordlund, E.; Krstic, M.; Bohn, T.; Smit, J.; Buchert, J.; Cirkovic Velickovic, T. Peanut Protein Structure, Polyphenol Content and Immune Response to Peanut Proteins: In Vivo Are Modulated by Laccase. Food and Function 2016, 7 (5), 2357–2366. https://doi.org/10.1039/c5fo01325a.
Food and Function
Royal Soc Chemistry, Cambridge..
Mihajlović-Lalić L, Radosavljević J, Nordlund E, Krstić-Ristivojević M, Bohn T, Smit J, Buchert J, Ćirković-Veličković T. Supplementary data for the article: Mihajlovic, L.; Radosavljevic, J.; Nordlund, E.; Krstic, M.; Bohn, T.; Smit, J.; Buchert, J.; Cirkovic Velickovic, T. Peanut Protein Structure, Polyphenol Content and Immune Response to Peanut Proteins: In Vivo Are Modulated by Laccase. Food and Function 2016, 7 (5), 2357–2366. https://doi.org/10.1039/c5fo01325a. Food and Function. 2016;
Mihajlović-Lalić Ljiljana, Radosavljević Jelena, Nordlund Emilia, Krstić-Ristivojević Maja, Bohn Torsten, Smit Joost, Buchert Johanna, Ćirković-Veličković Tanja, "Supplementary data for the article: Mihajlovic, L.; Radosavljevic, J.; Nordlund, E.; Krstic, M.; Bohn, T.; Smit, J.; Buchert, J.; Cirkovic Velickovic, T. Peanut Protein Structure, Polyphenol Content and Immune Response to Peanut Proteins: In Vivo Are Modulated by Laccase. Food and Function 2016, 7 (5), 2357–2366. https://doi.org/10.1039/c5fo01325a" Food and Function (2016)

Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina

Minić, Simeon L.; Stanić-Vučinić, Dragana; Mihailović-Vesić, Jelena; Krstić-Ristivojević, Maja; Nikolić, Milan; Ćirković-Veličković, Tanja

(Elsevier Science Bv, Amsterdam, 2016)

TY  - JOUR
AU  - Minić, Simeon L.
AU  - Stanić-Vučinić, Dragana
AU  - Mihailović-Vesić, Jelena
AU  - Krstić-Ristivojević, Maja
AU  - Nikolić, Milan
AU  - Ćirković-Veličković, Tanja
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2313
AB  - C-phycocyanin, the major protein of cyanobacteria Spirulina, possesses significant antioxidant, anti-cancer, anti-inflammatory and immunomodulatory effects, ascribed to covalently attached linear tetrapyrrole chromophore phycocyanobilin. There are no literature data about structure and biological activities of released peptides with bound chromophore in C-phycocyanin digest. This study aims to identify chromopeptides obtained after pepsin digestion of C-phycocyanin and to examine their bioactivities. C-phycocyanin is rapidly digested by pepsin in simulated gastric fluid. The structure of released chromopeptides was analyzed by high resolution tandem mass spectrometry and peptides varying in size from 2 to 13 amino acid residues were identified in both subunits of C-phycocyanin. Following separation by HPLC, chromopeptides were analyzed for potential bioactivities. It was shown that all five chromopeptide fractions have significant antioxidant and metal-chelating activities and show cytotoxic effect on human cervical adenocarcinoma and epithelial colonic cancer cell lines. In addition, chromopeptides protect human erythrocytes from free radical-induced hemolysis in antioxidative capacity dependant manner. There was a positive correlation between antioxidative potency and other biological activities of chromopeptides. Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin whose activity is mostly related to the antioxidative potency provided by chromophore. (C) 2016 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Proteomics
T1  - Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina
VL  - 147
SP  - 132
EP  - 139
DO  - 10.1016/j.jprot.2016.03.043
ER  - 
@article{
author = "Minić, Simeon L. and Stanić-Vučinić, Dragana and Mihailović-Vesić, Jelena and Krstić-Ristivojević, Maja and Nikolić, Milan and Ćirković-Veličković, Tanja",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2313",
abstract = "C-phycocyanin, the major protein of cyanobacteria Spirulina, possesses significant antioxidant, anti-cancer, anti-inflammatory and immunomodulatory effects, ascribed to covalently attached linear tetrapyrrole chromophore phycocyanobilin. There are no literature data about structure and biological activities of released peptides with bound chromophore in C-phycocyanin digest. This study aims to identify chromopeptides obtained after pepsin digestion of C-phycocyanin and to examine their bioactivities. C-phycocyanin is rapidly digested by pepsin in simulated gastric fluid. The structure of released chromopeptides was analyzed by high resolution tandem mass spectrometry and peptides varying in size from 2 to 13 amino acid residues were identified in both subunits of C-phycocyanin. Following separation by HPLC, chromopeptides were analyzed for potential bioactivities. It was shown that all five chromopeptide fractions have significant antioxidant and metal-chelating activities and show cytotoxic effect on human cervical adenocarcinoma and epithelial colonic cancer cell lines. In addition, chromopeptides protect human erythrocytes from free radical-induced hemolysis in antioxidative capacity dependant manner. There was a positive correlation between antioxidative potency and other biological activities of chromopeptides. Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin whose activity is mostly related to the antioxidative potency provided by chromophore. (C) 2016 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Proteomics",
title = "Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina",
volume = "147",
pages = "132-139",
doi = "10.1016/j.jprot.2016.03.043"
}
Minić, S. L., Stanić-Vučinić, D., Mihailović-Vesić, J., Krstić-Ristivojević, M., Nikolić, M.,& Ćirković-Veličković, T. (2016). Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina.
Journal of Proteomics
Elsevier Science Bv, Amsterdam., 147, 132-139.
https://doi.org/10.1016/j.jprot.2016.03.043
Minić SL, Stanić-Vučinić D, Mihailović-Vesić J, Krstić-Ristivojević M, Nikolić M, Ćirković-Veličković T. Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina. Journal of Proteomics. 2016;147:132-139
Minić Simeon L., Stanić-Vučinić Dragana, Mihailović-Vesić Jelena, Krstić-Ristivojević Maja, Nikolić Milan, Ćirković-Veličković Tanja, "Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina" Journal of Proteomics, 147 (2016):132-139,
https://doi.org/10.1016/j.jprot.2016.03.043 .
7
20
16
18

Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina

Minić, Simeon L.; Stanić-Vučinić, Dragana; Mihailović-Vesić, Jelena; Krstić-Ristivojević, Maja; Nikolić, Milan; Ćirković-Veličković, Tanja

(Elsevier Science Bv, Amsterdam, 2016)

TY  - JOUR
AU  - Minić, Simeon L.
AU  - Stanić-Vučinić, Dragana
AU  - Mihailović-Vesić, Jelena
AU  - Krstić-Ristivojević, Maja
AU  - Nikolić, Milan
AU  - Ćirković-Veličković, Tanja
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3444
AB  - C-phycocyanin, the major protein of cyanobacteria Spirulina, possesses significant antioxidant, anti-cancer, anti-inflammatory and immunomodulatory effects, ascribed to covalently attached linear tetrapyrrole chromophore phycocyanobilin. There are no literature data about structure and biological activities of released peptides with bound chromophore in C-phycocyanin digest. This study aims to identify chromopeptides obtained after pepsin digestion of C-phycocyanin and to examine their bioactivities. C-phycocyanin is rapidly digested by pepsin in simulated gastric fluid. The structure of released chromopeptides was analyzed by high resolution tandem mass spectrometry and peptides varying in size from 2 to 13 amino acid residues were identified in both subunits of C-phycocyanin. Following separation by HPLC, chromopeptides were analyzed for potential bioactivities. It was shown that all five chromopeptide fractions have significant antioxidant and metal-chelating activities and show cytotoxic effect on human cervical adenocarcinoma and epithelial colonic cancer cell lines. In addition, chromopeptides protect human erythrocytes from free radical-induced hemolysis in antioxidative capacity dependant manner. There was a positive correlation between antioxidative potency and other biological activities of chromopeptides. Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin whose activity is mostly related to the antioxidative potency provided by chromophore. (C) 2016 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Proteomics
T1  - Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina
VL  - 147
SP  - 132
EP  - 139
DO  - 10.1016/j.jprot.2016.03.043
ER  - 
@article{
author = "Minić, Simeon L. and Stanić-Vučinić, Dragana and Mihailović-Vesić, Jelena and Krstić-Ristivojević, Maja and Nikolić, Milan and Ćirković-Veličković, Tanja",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3444",
abstract = "C-phycocyanin, the major protein of cyanobacteria Spirulina, possesses significant antioxidant, anti-cancer, anti-inflammatory and immunomodulatory effects, ascribed to covalently attached linear tetrapyrrole chromophore phycocyanobilin. There are no literature data about structure and biological activities of released peptides with bound chromophore in C-phycocyanin digest. This study aims to identify chromopeptides obtained after pepsin digestion of C-phycocyanin and to examine their bioactivities. C-phycocyanin is rapidly digested by pepsin in simulated gastric fluid. The structure of released chromopeptides was analyzed by high resolution tandem mass spectrometry and peptides varying in size from 2 to 13 amino acid residues were identified in both subunits of C-phycocyanin. Following separation by HPLC, chromopeptides were analyzed for potential bioactivities. It was shown that all five chromopeptide fractions have significant antioxidant and metal-chelating activities and show cytotoxic effect on human cervical adenocarcinoma and epithelial colonic cancer cell lines. In addition, chromopeptides protect human erythrocytes from free radical-induced hemolysis in antioxidative capacity dependant manner. There was a positive correlation between antioxidative potency and other biological activities of chromopeptides. Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin whose activity is mostly related to the antioxidative potency provided by chromophore. (C) 2016 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Proteomics",
title = "Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina",
volume = "147",
pages = "132-139",
doi = "10.1016/j.jprot.2016.03.043"
}
Minić, S. L., Stanić-Vučinić, D., Mihailović-Vesić, J., Krstić-Ristivojević, M., Nikolić, M.,& Ćirković-Veličković, T. (2016). Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina.
Journal of Proteomics
Elsevier Science Bv, Amsterdam., 147, 132-139.
https://doi.org/10.1016/j.jprot.2016.03.043
Minić SL, Stanić-Vučinić D, Mihailović-Vesić J, Krstić-Ristivojević M, Nikolić M, Ćirković-Veličković T. Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina. Journal of Proteomics. 2016;147:132-139
Minić Simeon L., Stanić-Vučinić Dragana, Mihailović-Vesić Jelena, Krstić-Ristivojević Maja, Nikolić Milan, Ćirković-Veličković Tanja, "Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina" Journal of Proteomics, 147 (2016):132-139,
https://doi.org/10.1016/j.jprot.2016.03.043 .
7
20
16
18

Supplementary data for the article: Minic, S. L.; Stanic-Vucinic, D.; Mihailovic, J.; Krstic, M.; Nikolic, M. R.; Velickovic, T. C. Digestion by Pepsin Releases Biologically Active Chromopeptides from C-Phycocyanin, a Blue-Colored Biliprotein of Microalga Spirulina. J. Proteomics 2016, 147, 132–139. https://doi.org/10.1016/j.jprot.2016.03.043

Minić, Simeon L.; Stanić-Vučinić, Dragana; Mihailović-Vesić, Jelena; Krstić-Ristivojević, Maja; Nikolić, Milan; Ćirković-Veličković, Tanja

(Elsevier Science Bv, Amsterdam, 2016)

TY  - BOOK
AU  - Minić, Simeon L.
AU  - Stanić-Vučinić, Dragana
AU  - Mihailović-Vesić, Jelena
AU  - Krstić-Ristivojević, Maja
AU  - Nikolić, Milan
AU  - Ćirković-Veličković, Tanja
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3445
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Proteomics
T1  - Supplementary data for the article: Minic, S. L.; Stanic-Vucinic, D.; Mihailovic, J.; Krstic, M.; Nikolic, M. R.; Velickovic, T. C. Digestion by Pepsin Releases Biologically Active Chromopeptides from C-Phycocyanin, a Blue-Colored Biliprotein of Microalga Spirulina. J. Proteomics 2016, 147, 132–139. https://doi.org/10.1016/j.jprot.2016.03.043
ER  - 
@book{
author = "Minić, Simeon L. and Stanić-Vučinić, Dragana and Mihailović-Vesić, Jelena and Krstić-Ristivojević, Maja and Nikolić, Milan and Ćirković-Veličković, Tanja",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3445",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Proteomics",
title = "Supplementary data for the article: Minic, S. L.; Stanic-Vucinic, D.; Mihailovic, J.; Krstic, M.; Nikolic, M. R.; Velickovic, T. C. Digestion by Pepsin Releases Biologically Active Chromopeptides from C-Phycocyanin, a Blue-Colored Biliprotein of Microalga Spirulina. J. Proteomics 2016, 147, 132–139. https://doi.org/10.1016/j.jprot.2016.03.043"
}
Minić, S. L., Stanić-Vučinić, D., Mihailović-Vesić, J., Krstić-Ristivojević, M., Nikolić, M.,& Ćirković-Veličković, T. (2016). Supplementary data for the article: Minic, S. L.; Stanic-Vucinic, D.; Mihailovic, J.; Krstic, M.; Nikolic, M. R.; Velickovic, T. C. Digestion by Pepsin Releases Biologically Active Chromopeptides from C-Phycocyanin, a Blue-Colored Biliprotein of Microalga Spirulina. J. Proteomics 2016, 147, 132–139. https://doi.org/10.1016/j.jprot.2016.03.043.
Journal of Proteomics
Elsevier Science Bv, Amsterdam..
Minić SL, Stanić-Vučinić D, Mihailović-Vesić J, Krstić-Ristivojević M, Nikolić M, Ćirković-Veličković T. Supplementary data for the article: Minic, S. L.; Stanic-Vucinic, D.; Mihailovic, J.; Krstic, M.; Nikolic, M. R.; Velickovic, T. C. Digestion by Pepsin Releases Biologically Active Chromopeptides from C-Phycocyanin, a Blue-Colored Biliprotein of Microalga Spirulina. J. Proteomics 2016, 147, 132–139. https://doi.org/10.1016/j.jprot.2016.03.043. Journal of Proteomics. 2016;
Minić Simeon L., Stanić-Vučinić Dragana, Mihailović-Vesić Jelena, Krstić-Ristivojević Maja, Nikolić Milan, Ćirković-Veličković Tanja, "Supplementary data for the article: Minic, S. L.; Stanic-Vucinic, D.; Mihailovic, J.; Krstic, M.; Nikolic, M. R.; Velickovic, T. C. Digestion by Pepsin Releases Biologically Active Chromopeptides from C-Phycocyanin, a Blue-Colored Biliprotein of Microalga Spirulina. J. Proteomics 2016, 147, 132–139. https://doi.org/10.1016/j.jprot.2016.03.043" Journal of Proteomics (2016)

Peanut protein structure, polyphenol content and immune response to peanut proteins in vivo are modulated by laccase

Mihajlović-Lalić, Ljiljana; Radosavljević, Jelena; Nordlund, Emilia; Krstić-Ristivojević, Maja; Bohn, Torsten; Smit, Joost; Buchert, Johanna; Ćirković-Veličković, Tanja

(Royal Soc Chemistry, Cambridge, 2016)

TY  - JOUR
AU  - Mihajlović-Lalić, Ljiljana
AU  - Radosavljević, Jelena
AU  - Nordlund, Emilia
AU  - Krstić-Ristivojević, Maja
AU  - Bohn, Torsten
AU  - Smit, Joost
AU  - Buchert, Johanna
AU  - Ćirković-Veličković, Tanja
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2254
AB  - Food texture can be improved by enzyme-mediated covalent cross-linking of different food components, such as proteins and carbohydrates. Cross-linking changes the biological and immunological properties of proteins and may change the sensitizing potential of food allergens. In this study we applied a microbial polyphenol oxidase, laccase, to cross-link peanut proteins. The size and morphology of the obtained cross-linked proteins were analyzed by electrophoresis and electron microscopy. Structural changes in proteins were analyzed by CD spectroscopy and by using specific antibodies to major peanut allergens. The bioavailability of peanut proteins was analyzed using a Caco-2 epithelial cell model. The in vivo sensitizing potential of laccase-treated peanut proteins was analyzed using a mouse model of food allergy. Finally, peanut polyphenols were analyzed by UHPLC-MS/MS, before and after the enzymatic reaction with laccase. Laccase treatment of peanut proteins yielded a covalently cross-linked material, with the modified tertiary structure of peanut proteins, improved bioavailability of Ara h 2 (by 70 fold, p  lt  0.05) and modulated allergic immune response in vivo. The modulation of the immune response was related to the increased production of IgG2a antibodies 11 fold (p  lt  0.05) and reduced IL-13 secretion in in vitro cultured splenocytes 7 fold (p  lt  0.05). Analysis of the peanut polyphenol content and profile by HPLC-MS/MS revealed that laccase treatment depleted the peanut extract of polyphenol compounds leaving mostly isorhamnetin derivatives and procyanidin dimer B-type in detectable amounts. Treatment of complex food extracts rich in polyphenols with laccase results in both protein cross-linking and modification of polyphenol compounds. These extensively cross-linked proteins have unchanged potency to induce allergic sensitization in vivo, but certain immunomodulatory changes were observed.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food and Function
T1  - Peanut protein structure, polyphenol content and immune response to peanut proteins in vivo are modulated by laccase
VL  - 7
IS  - 5
SP  - 2357
EP  - 2366
DO  - 10.1039/c5fo01325a
ER  - 
@article{
author = "Mihajlović-Lalić, Ljiljana and Radosavljević, Jelena and Nordlund, Emilia and Krstić-Ristivojević, Maja and Bohn, Torsten and Smit, Joost and Buchert, Johanna and Ćirković-Veličković, Tanja",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2254",
abstract = "Food texture can be improved by enzyme-mediated covalent cross-linking of different food components, such as proteins and carbohydrates. Cross-linking changes the biological and immunological properties of proteins and may change the sensitizing potential of food allergens. In this study we applied a microbial polyphenol oxidase, laccase, to cross-link peanut proteins. The size and morphology of the obtained cross-linked proteins were analyzed by electrophoresis and electron microscopy. Structural changes in proteins were analyzed by CD spectroscopy and by using specific antibodies to major peanut allergens. The bioavailability of peanut proteins was analyzed using a Caco-2 epithelial cell model. The in vivo sensitizing potential of laccase-treated peanut proteins was analyzed using a mouse model of food allergy. Finally, peanut polyphenols were analyzed by UHPLC-MS/MS, before and after the enzymatic reaction with laccase. Laccase treatment of peanut proteins yielded a covalently cross-linked material, with the modified tertiary structure of peanut proteins, improved bioavailability of Ara h 2 (by 70 fold, p  lt  0.05) and modulated allergic immune response in vivo. The modulation of the immune response was related to the increased production of IgG2a antibodies 11 fold (p  lt  0.05) and reduced IL-13 secretion in in vitro cultured splenocytes 7 fold (p  lt  0.05). Analysis of the peanut polyphenol content and profile by HPLC-MS/MS revealed that laccase treatment depleted the peanut extract of polyphenol compounds leaving mostly isorhamnetin derivatives and procyanidin dimer B-type in detectable amounts. Treatment of complex food extracts rich in polyphenols with laccase results in both protein cross-linking and modification of polyphenol compounds. These extensively cross-linked proteins have unchanged potency to induce allergic sensitization in vivo, but certain immunomodulatory changes were observed.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food and Function",
title = "Peanut protein structure, polyphenol content and immune response to peanut proteins in vivo are modulated by laccase",
volume = "7",
number = "5",
pages = "2357-2366",
doi = "10.1039/c5fo01325a"
}
Mihajlović-Lalić, L., Radosavljević, J., Nordlund, E., Krstić-Ristivojević, M., Bohn, T., Smit, J., Buchert, J.,& Ćirković-Veličković, T. (2016). Peanut protein structure, polyphenol content and immune response to peanut proteins in vivo are modulated by laccase.
Food and Function
Royal Soc Chemistry, Cambridge., 7(5), 2357-2366.
https://doi.org/10.1039/c5fo01325a
Mihajlović-Lalić L, Radosavljević J, Nordlund E, Krstić-Ristivojević M, Bohn T, Smit J, Buchert J, Ćirković-Veličković T. Peanut protein structure, polyphenol content and immune response to peanut proteins in vivo are modulated by laccase. Food and Function. 2016;7(5):2357-2366
Mihajlović-Lalić Ljiljana, Radosavljević Jelena, Nordlund Emilia, Krstić-Ristivojević Maja, Bohn Torsten, Smit Joost, Buchert Johanna, Ćirković-Veličković Tanja, "Peanut protein structure, polyphenol content and immune response to peanut proteins in vivo are modulated by laccase" Food and Function, 7, no. 5 (2016):2357-2366,
https://doi.org/10.1039/c5fo01325a .
2
8
4
7

In vitro uptake of alpha-Gal containing protein by human monocyte derived dendritic cells

Tran, Thi Anh Thu; Grundström, Jeanette; Krstić-Ristivojević, Maja; Vukojević, Vesna; Apostolović, Danijela; Hamsten, Carl; Gafvelin, Guro; van Hage, Marianne

(Wiley-Blackwell, Hoboken, 2016)

TY  - CONF
AU  - Tran, Thi Anh Thu
AU  - Grundström, Jeanette
AU  - Krstić-Ristivojević, Maja
AU  - Vukojević, Vesna
AU  - Apostolović, Danijela
AU  - Hamsten, Carl
AU  - Gafvelin, Guro
AU  - van Hage, Marianne
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2309
PB  - Wiley-Blackwell, Hoboken
C3  - Allergy
T1  - In vitro uptake of alpha-Gal containing protein by human monocyte derived dendritic cells
VL  - 71
SP  - 284
EP  - 284
ER  - 
@conference{
author = "Tran, Thi Anh Thu and Grundström, Jeanette and Krstić-Ristivojević, Maja and Vukojević, Vesna and Apostolović, Danijela and Hamsten, Carl and Gafvelin, Guro and van Hage, Marianne",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2309",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "In vitro uptake of alpha-Gal containing protein by human monocyte derived dendritic cells",
volume = "71",
pages = "284-284"
}
Tran, T. A. T., Grundström, J., Krstić-Ristivojević, M., Vukojević, V., Apostolović, D., Hamsten, C., Gafvelin, G.,& van Hage, M. (2016). In vitro uptake of alpha-Gal containing protein by human monocyte derived dendritic cells.
Allergy
Wiley-Blackwell, Hoboken., 71, 284-284.
Tran TAT, Grundström J, Krstić-Ristivojević M, Vukojević V, Apostolović D, Hamsten C, Gafvelin G, van Hage M. In vitro uptake of alpha-Gal containing protein by human monocyte derived dendritic cells. Allergy. 2016;71:284-284
Tran Thi Anh Thu, Grundström Jeanette, Krstić-Ristivojević Maja, Vukojević Vesna, Apostolović Danijela, Hamsten Carl, Gafvelin Guro, van Hage Marianne, "In vitro uptake of alpha-Gal containing protein by human monocyte derived dendritic cells" Allergy, 71 (2016):284-284

alpha-Gal epitope on protein surface decreases transcytosis through Caco-2 monolayer

Krstić-Ristivojević, Maja; Apostolović, Danijela; Grundström, Jeanette; Tran, Thu T. A.; Ćirković-Veličković, Tanja; Hamsten, Carl; van Hage, Marianne

(Wiley-Blackwell, Hoboken, 2016)

TY  - CONF
AU  - Krstić-Ristivojević, Maja
AU  - Apostolović, Danijela
AU  - Grundström, Jeanette
AU  - Tran, Thu T. A.
AU  - Ćirković-Veličković, Tanja
AU  - Hamsten, Carl
AU  - van Hage, Marianne
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2310
PB  - Wiley-Blackwell, Hoboken
C3  - Allergy
T1  - alpha-Gal epitope on protein surface decreases transcytosis through Caco-2 monolayer
VL  - 71
SP  - 402
EP  - 402
ER  - 
@conference{
author = "Krstić-Ristivojević, Maja and Apostolović, Danijela and Grundström, Jeanette and Tran, Thu T. A. and Ćirković-Veličković, Tanja and Hamsten, Carl and van Hage, Marianne",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2310",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "alpha-Gal epitope on protein surface decreases transcytosis through Caco-2 monolayer",
volume = "71",
pages = "402-402"
}
Krstić-Ristivojević, M., Apostolović, D., Grundström, J., Tran, T. T. A., Ćirković-Veličković, T., Hamsten, C.,& van Hage, M. (2016). alpha-Gal epitope on protein surface decreases transcytosis through Caco-2 monolayer.
Allergy
Wiley-Blackwell, Hoboken., 71, 402-402.
Krstić-Ristivojević M, Apostolović D, Grundström J, Tran TTA, Ćirković-Veličković T, Hamsten C, van Hage M. alpha-Gal epitope on protein surface decreases transcytosis through Caco-2 monolayer. Allergy. 2016;71:402-402
Krstić-Ristivojević Maja, Apostolović Danijela, Grundström Jeanette, Tran Thu T. A., Ćirković-Veličković Tanja, Hamsten Carl, van Hage Marianne, "alpha-Gal epitope on protein surface decreases transcytosis through Caco-2 monolayer" Allergy, 71 (2016):402-402

Supplementary data for the article: Mitic-Culafic, D.; Nikolic, B.; Simin, N.; Jasnic, N.; Cetojevic-Simin, D.; Krstic, M.; Knezevic-Vukcevic, J. Effect of Allium Flavum L. and Allium Melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. Plant Food Hum. Nutr. 2016, 71 (1), 28–34. https://doi.org/10.1007/s11130-015-0519-0

Mitić-Ćulafić, Dragana; Nikolić, Biljana; Simin, Nataša; Jasnić, Nebojša; Četojević-Simin, Dragana; Krstić-Ristivojević, Maja; Knežević-Vukčević, Jelena

(Springer, Dordrecht, 2016)

TY  - BOOK
AU  - Mitić-Ćulafić, Dragana
AU  - Nikolić, Biljana
AU  - Simin, Nataša
AU  - Jasnić, Nebojša
AU  - Četojević-Simin, Dragana
AU  - Krstić-Ristivojević, Maja
AU  - Knežević-Vukčević, Jelena
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3482
PB  - Springer, Dordrecht
T2  - Plant Foods for Human Nutrition
T1  - Supplementary data for the article: Mitic-Culafic, D.; Nikolic, B.; Simin, N.; Jasnic, N.; Cetojevic-Simin, D.; Krstic, M.; Knezevic-Vukcevic, J. Effect of Allium Flavum L. and Allium Melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. Plant Food Hum. Nutr. 2016, 71 (1), 28–34. https://doi.org/10.1007/s11130-015-0519-0
ER  - 
@book{
author = "Mitić-Ćulafić, Dragana and Nikolić, Biljana and Simin, Nataša and Jasnić, Nebojša and Četojević-Simin, Dragana and Krstić-Ristivojević, Maja and Knežević-Vukčević, Jelena",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3482",
publisher = "Springer, Dordrecht",
journal = "Plant Foods for Human Nutrition",
title = "Supplementary data for the article: Mitic-Culafic, D.; Nikolic, B.; Simin, N.; Jasnic, N.; Cetojevic-Simin, D.; Krstic, M.; Knezevic-Vukcevic, J. Effect of Allium Flavum L. and Allium Melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. Plant Food Hum. Nutr. 2016, 71 (1), 28–34. https://doi.org/10.1007/s11130-015-0519-0"
}
Mitić-Ćulafić, D., Nikolić, B., Simin, N., Jasnić, N., Četojević-Simin, D., Krstić-Ristivojević, M.,& Knežević-Vukčević, J. (2016). Supplementary data for the article: Mitic-Culafic, D.; Nikolic, B.; Simin, N.; Jasnic, N.; Cetojevic-Simin, D.; Krstic, M.; Knezevic-Vukcevic, J. Effect of Allium Flavum L. and Allium Melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. Plant Food Hum. Nutr. 2016, 71 (1), 28–34. https://doi.org/10.1007/s11130-015-0519-0.
Plant Foods for Human Nutrition
Springer, Dordrecht..
Mitić-Ćulafić D, Nikolić B, Simin N, Jasnić N, Četojević-Simin D, Krstić-Ristivojević M, Knežević-Vukčević J. Supplementary data for the article: Mitic-Culafic, D.; Nikolic, B.; Simin, N.; Jasnic, N.; Cetojevic-Simin, D.; Krstic, M.; Knezevic-Vukcevic, J. Effect of Allium Flavum L. and Allium Melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. Plant Food Hum. Nutr. 2016, 71 (1), 28–34. https://doi.org/10.1007/s11130-015-0519-0. Plant Foods for Human Nutrition. 2016;
Mitić-Ćulafić Dragana, Nikolić Biljana, Simin Nataša, Jasnić Nebojša, Četojević-Simin Dragana, Krstić-Ristivojević Maja, Knežević-Vukčević Jelena, "Supplementary data for the article: Mitic-Culafic, D.; Nikolic, B.; Simin, N.; Jasnic, N.; Cetojevic-Simin, D.; Krstic, M.; Knezevic-Vukcevic, J. Effect of Allium Flavum L. and Allium Melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. Plant Food Hum. Nutr. 2016, 71 (1), 28–34. https://doi.org/10.1007/s11130-015-0519-0" Plant Foods for Human Nutrition (2016)

Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase

Mitić-Ćulafić, Dragana; Nikolić, Biljana; Simin, Nataša; Jasnić, Nebojša; Četojević-Simin, Dragana; Krstić-Ristivojević, Maja; Knežević-Vukčević, Jelena

(Springer, Dordrecht, 2016)

TY  - JOUR
AU  - Mitić-Ćulafić, Dragana
AU  - Nikolić, Biljana
AU  - Simin, Nataša
AU  - Jasnić, Nebojša
AU  - Četojević-Simin, Dragana
AU  - Krstić-Ristivojević, Maja
AU  - Knežević-Vukčević, Jelena
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2065
AB  - Allium flavum L. and Allium melanantherum Pan. are wild growing plants used in traditional diet in Balkan region. While chemical composition and some biological activities of A. flavum have been reported, A. melanantherum, as an endemic in the Balkan Peninsula, has never been comprehensively examined. After chemical characterization of A. melanantherum, we examined the protective effect of methanol extracts of both species against t-butyl hydro-peroxide (t-BOOH)-induced DNA damage and mutagenesis. The bacterial reverse mutation assay was performed on Escherichia coli WP2 oxyR strain. DNA damage was monitored in human fetal lung fibroblasts (MRC-5) with alkaline comet assay. Obtained results indicated that extracts reduced t-BOOH-induced DNA damage up to 70 and 72 % for A. flavum and A. melanantherum extract, respectively, and showed no effect on t-BOOH-induced mutagenesis. Since the results indicated modulatory effect on cell-mediated antioxidative defense, the effect of extracts on total protein content, and superoxide dismutase (SOD) and catalase (CAT) amounts and activities were monitored. Both extracts increased total protein content, while the increase of enzyme amount and activity was obtained only with A. melanantherum extract and restricted to CAT. The activity of CuZnSOD family was not affected, while SOD1 and SOD2 amounts were significantly decreased, indicating potential involvement of extracellular CuZnSOD. Obtained results strongly support the traditional use of A. flavum and A. melanantherum in nutrition and recommend them for further study.
PB  - Springer, Dordrecht
T2  - Plant Foods for Human Nutrition
T1  - Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase
VL  - 71
IS  - 1
SP  - 28
EP  - 34
DO  - 10.1007/s11130-015-0519-0
ER  - 
@article{
author = "Mitić-Ćulafić, Dragana and Nikolić, Biljana and Simin, Nataša and Jasnić, Nebojša and Četojević-Simin, Dragana and Krstić-Ristivojević, Maja and Knežević-Vukčević, Jelena",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2065",
abstract = "Allium flavum L. and Allium melanantherum Pan. are wild growing plants used in traditional diet in Balkan region. While chemical composition and some biological activities of A. flavum have been reported, A. melanantherum, as an endemic in the Balkan Peninsula, has never been comprehensively examined. After chemical characterization of A. melanantherum, we examined the protective effect of methanol extracts of both species against t-butyl hydro-peroxide (t-BOOH)-induced DNA damage and mutagenesis. The bacterial reverse mutation assay was performed on Escherichia coli WP2 oxyR strain. DNA damage was monitored in human fetal lung fibroblasts (MRC-5) with alkaline comet assay. Obtained results indicated that extracts reduced t-BOOH-induced DNA damage up to 70 and 72 % for A. flavum and A. melanantherum extract, respectively, and showed no effect on t-BOOH-induced mutagenesis. Since the results indicated modulatory effect on cell-mediated antioxidative defense, the effect of extracts on total protein content, and superoxide dismutase (SOD) and catalase (CAT) amounts and activities were monitored. Both extracts increased total protein content, while the increase of enzyme amount and activity was obtained only with A. melanantherum extract and restricted to CAT. The activity of CuZnSOD family was not affected, while SOD1 and SOD2 amounts were significantly decreased, indicating potential involvement of extracellular CuZnSOD. Obtained results strongly support the traditional use of A. flavum and A. melanantherum in nutrition and recommend them for further study.",
publisher = "Springer, Dordrecht",
journal = "Plant Foods for Human Nutrition",
title = "Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase",
volume = "71",
number = "1",
pages = "28-34",
doi = "10.1007/s11130-015-0519-0"
}
Mitić-Ćulafić, D., Nikolić, B., Simin, N., Jasnić, N., Četojević-Simin, D., Krstić-Ristivojević, M.,& Knežević-Vukčević, J. (2016). Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase.
Plant Foods for Human Nutrition
Springer, Dordrecht., 71(1), 28-34.
https://doi.org/10.1007/s11130-015-0519-0
Mitić-Ćulafić D, Nikolić B, Simin N, Jasnić N, Četojević-Simin D, Krstić-Ristivojević M, Knežević-Vukčević J. Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. Plant Foods for Human Nutrition. 2016;71(1):28-34
Mitić-Ćulafić Dragana, Nikolić Biljana, Simin Nataša, Jasnić Nebojša, Četojević-Simin Dragana, Krstić-Ristivojević Maja, Knežević-Vukčević Jelena, "Effect of Allium flavum L. and Allium melanantherum Pan. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase" Plant Foods for Human Nutrition, 71, no. 1 (2016):28-34,
https://doi.org/10.1007/s11130-015-0519-0 .
1
6
4
5

Supplementary data for article: Krstić-Ristivojević, M.; Stojadinović, M. M.; Smiljanić, K.; Stanić-Vučinić, D.; Ćirković-Veličković, T. The Anti-Cancer Activity of Green Tea, Coffee and Cocoa Extracts on Human Cervical Adenocarcinoma HeLa Cells Depends on Both pro-Oxidant and Anti-Proliferative Activities of Polyphenols. RSC Advances 2015, 5 (5), 3260–3268. https://doi.org/10.1039/c4ra13230k

Krstić-Ristivojević, Maja; Stojadinović, Marija M.; Smiljanić, Katarina; Stanić-Vučinić, Dragana; Ćirković-Veličković, Tanja

(Royal Soc Chemistry, Cambridge, 2015)

TY  - BOOK
AU  - Krstić-Ristivojević, Maja
AU  - Stojadinović, Marija M.
AU  - Smiljanić, Katarina
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3449
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - Supplementary data for article: Krstić-Ristivojević, M.; Stojadinović, M. M.; Smiljanić, K.; Stanić-Vučinić, D.; Ćirković-Veličković, T. The Anti-Cancer Activity of Green Tea, Coffee and Cocoa Extracts on Human Cervical Adenocarcinoma HeLa Cells Depends on Both pro-Oxidant and Anti-Proliferative Activities of Polyphenols. RSC Advances 2015, 5 (5), 3260–3268. https://doi.org/10.1039/c4ra13230k
ER  - 
@book{
author = "Krstić-Ristivojević, Maja and Stojadinović, Marija M. and Smiljanić, Katarina and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2015",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3449",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "Supplementary data for article: Krstić-Ristivojević, M.; Stojadinović, M. M.; Smiljanić, K.; Stanić-Vučinić, D.; Ćirković-Veličković, T. The Anti-Cancer Activity of Green Tea, Coffee and Cocoa Extracts on Human Cervical Adenocarcinoma HeLa Cells Depends on Both pro-Oxidant and Anti-Proliferative Activities of Polyphenols. RSC Advances 2015, 5 (5), 3260–3268. https://doi.org/10.1039/c4ra13230k"
}
Krstić-Ristivojević, M., Stojadinović, M. M., Smiljanić, K., Stanić-Vučinić, D.,& Ćirković-Veličković, T. (2015). Supplementary data for article: Krstić-Ristivojević, M.; Stojadinović, M. M.; Smiljanić, K.; Stanić-Vučinić, D.; Ćirković-Veličković, T. The Anti-Cancer Activity of Green Tea, Coffee and Cocoa Extracts on Human Cervical Adenocarcinoma HeLa Cells Depends on Both pro-Oxidant and Anti-Proliferative Activities of Polyphenols. RSC Advances 2015, 5 (5), 3260–3268. https://doi.org/10.1039/c4ra13230k.
RSC Advances
Royal Soc Chemistry, Cambridge..
Krstić-Ristivojević M, Stojadinović MM, Smiljanić K, Stanić-Vučinić D, Ćirković-Veličković T. Supplementary data for article: Krstić-Ristivojević, M.; Stojadinović, M. M.; Smiljanić, K.; Stanić-Vučinić, D.; Ćirković-Veličković, T. The Anti-Cancer Activity of Green Tea, Coffee and Cocoa Extracts on Human Cervical Adenocarcinoma HeLa Cells Depends on Both pro-Oxidant and Anti-Proliferative Activities of Polyphenols. RSC Advances 2015, 5 (5), 3260–3268. https://doi.org/10.1039/c4ra13230k. RSC Advances. 2015;
Krstić-Ristivojević Maja, Stojadinović Marija M., Smiljanić Katarina, Stanić-Vučinić Dragana, Ćirković-Veličković Tanja, "Supplementary data for article: Krstić-Ristivojević, M.; Stojadinović, M. M.; Smiljanić, K.; Stanić-Vučinić, D.; Ćirković-Veličković, T. The Anti-Cancer Activity of Green Tea, Coffee and Cocoa Extracts on Human Cervical Adenocarcinoma HeLa Cells Depends on Both pro-Oxidant and Anti-Proliferative Activities of Polyphenols. RSC Advances 2015, 5 (5), 3260–3268. https://doi.org/10.1039/c4ra13230k" RSC Advances (2015)

The anti-cancer activity of green tea, coffee and cocoa extracts on human cervical adenocarcinoma HeLa cells depends on both pro-oxidant and anti-proliferative activities of polyphenols

Krstić-Ristivojević, Maja; Stojadinović, Marija M.; Smiljanić, Katarina; Stanić-Vučinić, Dragana; Ćirković-Veličković, Tanja

(Royal Soc Chemistry, Cambridge, 2015)

TY  - JOUR
AU  - Krstić-Ristivojević, Maja
AU  - Stojadinović, Marija M.
AU  - Smiljanić, Katarina
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1888
AB  - It has been shown before that dietary polyphenols possess cancer chemopreventive effects. As cervical cancer is the second leading genital malignancy in women after breast cancer, the anti-cervical cancer effects of polyphenol extracts of commonly used beverages (green tea, coffee and cocoa) were tested and compared in HeLa cells. All the extracts induced apoptosis of HeLa cells, but green tea was the most potent. However, as opposed to green tea which induced a strong anti-proliferative response in HeLa cells, coffee and cocoa extracts promoted the proliferation of surviving cells. After short-term exposure, green tea and coffee extracts, but not cocoa, induced the formation of intracellular reactive oxygen species. Only the green tea extract increased the production of superoxide anion radicals and decreased reduced glutathione levels. Gene expression of Cu/Zn and Mn-superoxide dismutase or catalase was unaltered in cells treated with extracts, but green tea partially inhibited catalase activity. The cytotoxic activity of green tea and coffee extracts was partially inhibited by vitamin C. The in vitro anti-cervical cancer potency of tested polyphenol extracts is related to their pro-oxidant and anti-proliferative activities and are exhibited in the following order: green tea  gt  coffee  gt  cocoa, with only green tea showing both pro-oxidative and anti-proliferative action.
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - The anti-cancer activity of green tea, coffee and cocoa extracts on human cervical adenocarcinoma HeLa cells depends on both pro-oxidant and anti-proliferative activities of polyphenols
VL  - 5
IS  - 5
SP  - 3260
EP  - 3268
DO  - 10.1039/c4ra13230k
ER  - 
@article{
author = "Krstić-Ristivojević, Maja and Stojadinović, Marija M. and Smiljanić, Katarina and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2015",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1888",
abstract = "It has been shown before that dietary polyphenols possess cancer chemopreventive effects. As cervical cancer is the second leading genital malignancy in women after breast cancer, the anti-cervical cancer effects of polyphenol extracts of commonly used beverages (green tea, coffee and cocoa) were tested and compared in HeLa cells. All the extracts induced apoptosis of HeLa cells, but green tea was the most potent. However, as opposed to green tea which induced a strong anti-proliferative response in HeLa cells, coffee and cocoa extracts promoted the proliferation of surviving cells. After short-term exposure, green tea and coffee extracts, but not cocoa, induced the formation of intracellular reactive oxygen species. Only the green tea extract increased the production of superoxide anion radicals and decreased reduced glutathione levels. Gene expression of Cu/Zn and Mn-superoxide dismutase or catalase was unaltered in cells treated with extracts, but green tea partially inhibited catalase activity. The cytotoxic activity of green tea and coffee extracts was partially inhibited by vitamin C. The in vitro anti-cervical cancer potency of tested polyphenol extracts is related to their pro-oxidant and anti-proliferative activities and are exhibited in the following order: green tea  gt  coffee  gt  cocoa, with only green tea showing both pro-oxidative and anti-proliferative action.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "The anti-cancer activity of green tea, coffee and cocoa extracts on human cervical adenocarcinoma HeLa cells depends on both pro-oxidant and anti-proliferative activities of polyphenols",
volume = "5",
number = "5",
pages = "3260-3268",
doi = "10.1039/c4ra13230k"
}
Krstić-Ristivojević, M., Stojadinović, M. M., Smiljanić, K., Stanić-Vučinić, D.,& Ćirković-Veličković, T. (2015). The anti-cancer activity of green tea, coffee and cocoa extracts on human cervical adenocarcinoma HeLa cells depends on both pro-oxidant and anti-proliferative activities of polyphenols.
RSC Advances
Royal Soc Chemistry, Cambridge., 5(5), 3260-3268.
https://doi.org/10.1039/c4ra13230k
Krstić-Ristivojević M, Stojadinović MM, Smiljanić K, Stanić-Vučinić D, Ćirković-Veličković T. The anti-cancer activity of green tea, coffee and cocoa extracts on human cervical adenocarcinoma HeLa cells depends on both pro-oxidant and anti-proliferative activities of polyphenols. RSC Advances. 2015;5(5):3260-3268
Krstić-Ristivojević Maja, Stojadinović Marija M., Smiljanić Katarina, Stanić-Vučinić Dragana, Ćirković-Veličković Tanja, "The anti-cancer activity of green tea, coffee and cocoa extracts on human cervical adenocarcinoma HeLa cells depends on both pro-oxidant and anti-proliferative activities of polyphenols" RSC Advances, 5, no. 5 (2015):3260-3268,
https://doi.org/10.1039/c4ra13230k .
1
19
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17

Pepsin digestion of C-phycocyanin releases chromopeptides with potent anticancer and antioxidant activities

Minić, Simeon L.; Krstić-Ristivojević, Maja; Apostolović, Danijela; Mihailović-Vesić, Jelena; Stanić-Vučinić, Dragana; Nikolić, Milan; Ćirković-Veličković, Tanja

(Wiley-Blackwell, Hoboken, 2015)

TY  - CONF
AU  - Minić, Simeon L.
AU  - Krstić-Ristivojević, Maja
AU  - Apostolović, Danijela
AU  - Mihailović-Vesić, Jelena
AU  - Stanić-Vučinić, Dragana
AU  - Nikolić, Milan
AU  - Ćirković-Veličković, Tanja
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1976
PB  - Wiley-Blackwell, Hoboken
C3  - FEBS Journal / Federation of European of Biochemical Societies
T1  - Pepsin digestion of C-phycocyanin releases chromopeptides with potent anticancer and antioxidant activities
VL  - 282
SP  - 134
EP  - 135
ER  - 
@conference{
author = "Minić, Simeon L. and Krstić-Ristivojević, Maja and Apostolović, Danijela and Mihailović-Vesić, Jelena and Stanić-Vučinić, Dragana and Nikolić, Milan and Ćirković-Veličković, Tanja",
year = "2015",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1976",
publisher = "Wiley-Blackwell, Hoboken",
journal = "FEBS Journal / Federation of European of Biochemical Societies",
title = "Pepsin digestion of C-phycocyanin releases chromopeptides with potent anticancer and antioxidant activities",
volume = "282",
pages = "134-135"
}
Minić, S. L., Krstić-Ristivojević, M., Apostolović, D., Mihailović-Vesić, J., Stanić-Vučinić, D., Nikolić, M.,& Ćirković-Veličković, T. (2015). Pepsin digestion of C-phycocyanin releases chromopeptides with potent anticancer and antioxidant activities.
FEBS Journal / Federation of European of Biochemical Societies
Wiley-Blackwell, Hoboken., 282, 134-135.
Minić SL, Krstić-Ristivojević M, Apostolović D, Mihailović-Vesić J, Stanić-Vučinić D, Nikolić M, Ćirković-Veličković T. Pepsin digestion of C-phycocyanin releases chromopeptides with potent anticancer and antioxidant activities. FEBS Journal / Federation of European of Biochemical Societies. 2015;282:134-135
Minić Simeon L., Krstić-Ristivojević Maja, Apostolović Danijela, Mihailović-Vesić Jelena, Stanić-Vučinić Dragana, Nikolić Milan, Ćirković-Veličković Tanja, "Pepsin digestion of C-phycocyanin releases chromopeptides with potent anticancer and antioxidant activities" FEBS Journal / Federation of European of Biochemical Societies, 282 (2015):134-135
1

Green tea, coffee and cocoa polyphenols exhibit different effects on HeLa cell viability and proliferation

Krstić-Ristivojević, Maja; Stojadinović, Marija M.; Smiljanić, Katarina; Stanić-Vučinić, Dragana; Ćirković-Veličković, Tanja

(Wiley-Blackwell, Hoboken, 2014)

TY  - CONF
AU  - Krstić-Ristivojević, Maja
AU  - Stojadinović, Marija M.
AU  - Smiljanić, Katarina
AU  - Stanić-Vučinić, Dragana
AU  - Ćirković-Veličković, Tanja
PY  - 2014
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1754
PB  - Wiley-Blackwell, Hoboken
C3  - FEBS Journal / Federation of European of Biochemical Societies
T1  - Green tea, coffee and cocoa polyphenols exhibit different effects on HeLa cell viability and proliferation
VL  - 281
SP  - 72
EP  - 72
ER  - 
@conference{
author = "Krstić-Ristivojević, Maja and Stojadinović, Marija M. and Smiljanić, Katarina and Stanić-Vučinić, Dragana and Ćirković-Veličković, Tanja",
year = "2014",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1754",
publisher = "Wiley-Blackwell, Hoboken",
journal = "FEBS Journal / Federation of European of Biochemical Societies",
title = "Green tea, coffee and cocoa polyphenols exhibit different effects on HeLa cell viability and proliferation",
volume = "281",
pages = "72-72"
}
Krstić-Ristivojević, M., Stojadinović, M. M., Smiljanić, K., Stanić-Vučinić, D.,& Ćirković-Veličković, T. (2014). Green tea, coffee and cocoa polyphenols exhibit different effects on HeLa cell viability and proliferation.
FEBS Journal / Federation of European of Biochemical Societies
Wiley-Blackwell, Hoboken., 281, 72-72.
Krstić-Ristivojević M, Stojadinović MM, Smiljanić K, Stanić-Vučinić D, Ćirković-Veličković T. Green tea, coffee and cocoa polyphenols exhibit different effects on HeLa cell viability and proliferation. FEBS Journal / Federation of European of Biochemical Societies. 2014;281:72-72
Krstić-Ristivojević Maja, Stojadinović Marija M., Smiljanić Katarina, Stanić-Vučinić Dragana, Ćirković-Veličković Tanja, "Green tea, coffee and cocoa polyphenols exhibit different effects on HeLa cell viability and proliferation" FEBS Journal / Federation of European of Biochemical Societies, 281 (2014):72-72

Sensitizing potential of enzymatically cross-linked peanut proteins in a mouse model of peanut allergy

Radosavljević, Jelena; Nordlund, Emilia; Mihajlovic, Luka; Krstić-Ristivojević, Maja; Bohn, Torsten; Buchert, Johanna; Ćirković-Veličković, Tanja; Smit, Joost

(Wiley-Blackwell, Hoboken, 2014)

TY  - JOUR
AU  - Radosavljević, Jelena
AU  - Nordlund, Emilia
AU  - Mihajlovic, Luka
AU  - Krstić-Ristivojević, Maja
AU  - Bohn, Torsten
AU  - Buchert, Johanna
AU  - Ćirković-Veličković, Tanja
AU  - Smit, Joost
PY  - 2014
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1510
AB  - ScopeThe cross-linking of proteins by enzymes to form high-molecular-weight protein, aggregates can be used to tailor the technological or physiological functionality of food products. Aggregation of dietary proteins by food processing may promote allergic sensitization, but the effects of enzymatic cross-linking of dietary proteins on the allergenic potential of food are not known. In this study, the bioavailability and the sensitizing or tolerizing potential of peanut proteins (PE) cross-linked with microbial tyrosinase from Trichoderma reesei and mushroom tyrosinase from Agaricus bisporus, were investigated. Methods and resultsThe impact of cross-linking of PE on the in vitro bioavailability of fluorescein isothiocyanate-labeled peanut proteins was tested in a Caco-2 cell monolayer and by competitive ELISA. The in vivo allergenicity or capacity to induce oral tolerance in mice were measured by serum levels of PE-specific antibodies and T cell cytokine production after exposure to PE and cross-linked PE. ConclusionEnzymatic processing of peanut proteins by the two tyrosinases increased the bioavailability of major peanut allergen Ara h 2, but did not significantly change the allergenic or tolerizing properties of peanut. Enzymatic treatment of peanut proteins yielded cross-linked proteins with preserved molecular and immunological features of peanut allergens.
PB  - Wiley-Blackwell, Hoboken
T2  - Molecular Nutrition and Food Research
T1  - Sensitizing potential of enzymatically cross-linked peanut proteins in a mouse model of peanut allergy
VL  - 58
IS  - 3
SP  - 635
EP  - 646
DO  - 10.1002/mnfr.201300403
ER  - 
@article{
author = "Radosavljević, Jelena and Nordlund, Emilia and Mihajlovic, Luka and Krstić-Ristivojević, Maja and Bohn, Torsten and Buchert, Johanna and Ćirković-Veličković, Tanja and Smit, Joost",
year = "2014",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1510",
abstract = "ScopeThe cross-linking of proteins by enzymes to form high-molecular-weight protein, aggregates can be used to tailor the technological or physiological functionality of food products. Aggregation of dietary proteins by food processing may promote allergic sensitization, but the effects of enzymatic cross-linking of dietary proteins on the allergenic potential of food are not known. In this study, the bioavailability and the sensitizing or tolerizing potential of peanut proteins (PE) cross-linked with microbial tyrosinase from Trichoderma reesei and mushroom tyrosinase from Agaricus bisporus, were investigated. Methods and resultsThe impact of cross-linking of PE on the in vitro bioavailability of fluorescein isothiocyanate-labeled peanut proteins was tested in a Caco-2 cell monolayer and by competitive ELISA. The in vivo allergenicity or capacity to induce oral tolerance in mice were measured by serum levels of PE-specific antibodies and T cell cytokine production after exposure to PE and cross-linked PE. ConclusionEnzymatic processing of peanut proteins by the two tyrosinases increased the bioavailability of major peanut allergen Ara h 2, but did not significantly change the allergenic or tolerizing properties of peanut. Enzymatic treatment of peanut proteins yielded cross-linked proteins with preserved molecular and immunological features of peanut allergens.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Molecular Nutrition and Food Research",
title = "Sensitizing potential of enzymatically cross-linked peanut proteins in a mouse model of peanut allergy",
volume = "58",
number = "3",
pages = "635-646",
doi = "10.1002/mnfr.201300403"
}
Radosavljević, J., Nordlund, E., Mihajlovic, L., Krstić-Ristivojević, M., Bohn, T., Buchert, J., Ćirković-Veličković, T.,& Smit, J. (2014). Sensitizing potential of enzymatically cross-linked peanut proteins in a mouse model of peanut allergy.
Molecular Nutrition and Food Research
Wiley-Blackwell, Hoboken., 58(3), 635-646.
https://doi.org/10.1002/mnfr.201300403
Radosavljević J, Nordlund E, Mihajlovic L, Krstić-Ristivojević M, Bohn T, Buchert J, Ćirković-Veličković T, Smit J. Sensitizing potential of enzymatically cross-linked peanut proteins in a mouse model of peanut allergy. Molecular Nutrition and Food Research. 2014;58(3):635-646
Radosavljević Jelena, Nordlund Emilia, Mihajlovic Luka, Krstić-Ristivojević Maja, Bohn Torsten, Buchert Johanna, Ćirković-Veličković Tanja, Smit Joost, "Sensitizing potential of enzymatically cross-linked peanut proteins in a mouse model of peanut allergy" Molecular Nutrition and Food Research, 58, no. 3 (2014):635-646,
https://doi.org/10.1002/mnfr.201300403 .
8
24
22
24

Second Generation Steroidal 4-Aminoquinolines Are Potent, Dual-Target Inhibitors of the Botulinum Neurotoxin Serotype A Metalloprotease and P. falciparum Malaria

Selaković, Milica; Opsenica, Dejan M.; Burnett, James C.; Gomba, Laura; Nuss, Jonathan E.; Selaković, Života; Konstantinović, Jelena M.; Krstić-Ristivojević, Maja; Šegan, Sandra B.; Zlatović, Mario; Sciotti, Richard J.; Bavari, Sina; Šolaja, Bogdan A.

(Amer Chemical Soc, Washington, 2014)

TY  - JOUR
AU  - Selaković, Milica
AU  - Opsenica, Dejan M.
AU  - Burnett, James C.
AU  - Gomba, Laura
AU  - Nuss, Jonathan E.
AU  - Selaković, Života
AU  - Konstantinović, Jelena M.
AU  - Krstić-Ristivojević, Maja
AU  - Šegan, Sandra B.
AU  - Zlatović, Mario
AU  - Sciotti, Richard J.
AU  - Bavari, Sina
AU  - Šolaja, Bogdan A.
PY  - 2014
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1781
AB  - Significantly more potent second generation 4-amino-7-chloroquinoline (4,7-ACQ) based inhibitors of the botulinum neurotoxin serotype A (BoNT/A) light chain were synthesized. Introducing an amino group at the C(3) position of the cholate component markedly increased potency (IC50 values for such derivatives ranged from 0.81 to 2.27 mu M). Two additional subclasses were prepared: bis(steroidal)-4,7-ACQ derivatives and bis(4,7-ACQ)cholate derivatives; both classes provided inhibitors with nanomolar-range potencies (e.g., the K-i of compound 67 is 0.10 mu M). During BoNT/A challenge using primary neurons, select derivatives protected SNAP-25 by up to 89%. Docking simulations were performed to rationalize the compounds' in vitro potencies. In addition to specific residue contacts, coordination of the enzyme's catalytic zinc and expulsion of the enzyme's catalytic water were a consistent theme. With respect to antimalarial activity, the compounds provided better IC90 activities against chloroquine resistant (CQR) malaria than CQ, and seven compounds were more active than mefloquine against CQR strain W2.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Medicinal Chemistry
T1  - Second Generation Steroidal 4-Aminoquinolines Are Potent, Dual-Target Inhibitors of the Botulinum Neurotoxin Serotype A Metalloprotease and P. falciparum Malaria
VL  - 57
IS  - 10
SP  - 4134
EP  - 4153
DO  - 10.1021/jm500033r
ER  - 
@article{
author = "Selaković, Milica and Opsenica, Dejan M. and Burnett, James C. and Gomba, Laura and Nuss, Jonathan E. and Selaković, Života and Konstantinović, Jelena M. and Krstić-Ristivojević, Maja and Šegan, Sandra B. and Zlatović, Mario and Sciotti, Richard J. and Bavari, Sina and Šolaja, Bogdan A.",
year = "2014",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1781",
abstract = "Significantly more potent second generation 4-amino-7-chloroquinoline (4,7-ACQ) based inhibitors of the botulinum neurotoxin serotype A (BoNT/A) light chain were synthesized. Introducing an amino group at the C(3) position of the cholate component markedly increased potency (IC50 values for such derivatives ranged from 0.81 to 2.27 mu M). Two additional subclasses were prepared: bis(steroidal)-4,7-ACQ derivatives and bis(4,7-ACQ)cholate derivatives; both classes provided inhibitors with nanomolar-range potencies (e.g., the K-i of compound 67 is 0.10 mu M). During BoNT/A challenge using primary neurons, select derivatives protected SNAP-25 by up to 89%. Docking simulations were performed to rationalize the compounds' in vitro potencies. In addition to specific residue contacts, coordination of the enzyme's catalytic zinc and expulsion of the enzyme's catalytic water were a consistent theme. With respect to antimalarial activity, the compounds provided better IC90 activities against chloroquine resistant (CQR) malaria than CQ, and seven compounds were more active than mefloquine against CQR strain W2.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Medicinal Chemistry",
title = "Second Generation Steroidal 4-Aminoquinolines Are Potent, Dual-Target Inhibitors of the Botulinum Neurotoxin Serotype A Metalloprotease and P. falciparum Malaria",
volume = "57",
number = "10",
pages = "4134-4153",
doi = "10.1021/jm500033r"
}
Selaković, M., Opsenica, D. M., Burnett, J. C., Gomba, L., Nuss, J. E., Selaković, Ž., Konstantinović, J. M., Krstić-Ristivojević, M., Šegan, S. B., Zlatović, M., Sciotti, R. J., Bavari, S.,& Šolaja, B. A. (2014). Second Generation Steroidal 4-Aminoquinolines Are Potent, Dual-Target Inhibitors of the Botulinum Neurotoxin Serotype A Metalloprotease and P. falciparum Malaria.
Journal of Medicinal Chemistry
Amer Chemical Soc, Washington., 57(10), 4134-4153.
https://doi.org/10.1021/jm500033r
Selaković M, Opsenica DM, Burnett JC, Gomba L, Nuss JE, Selaković Ž, Konstantinović JM, Krstić-Ristivojević M, Šegan SB, Zlatović M, Sciotti RJ, Bavari S, Šolaja BA. Second Generation Steroidal 4-Aminoquinolines Are Potent, Dual-Target Inhibitors of the Botulinum Neurotoxin Serotype A Metalloprotease and P. falciparum Malaria. Journal of Medicinal Chemistry. 2014;57(10):4134-4153
Selaković Milica, Opsenica Dejan M., Burnett James C., Gomba Laura, Nuss Jonathan E., Selaković Života, Konstantinović Jelena M., Krstić-Ristivojević Maja, Šegan Sandra B., Zlatović Mario, Sciotti Richard J., Bavari Sina, Šolaja Bogdan A., "Second Generation Steroidal 4-Aminoquinolines Are Potent, Dual-Target Inhibitors of the Botulinum Neurotoxin Serotype A Metalloprotease and P. falciparum Malaria" Journal of Medicinal Chemistry, 57, no. 10 (2014):4134-4153,
https://doi.org/10.1021/jm500033r .
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Maghemite and poly-DL-alanine based core-shell multifunctional nanohybrids for environmental protection and biomedicine applications

Covaliu, Cristina Ileana; Paraschiv, Gigel; Biris, Sorin-Stefan; Jitaru, Ioana; Vasile, Eugeniu; Diamandescu, Lucian; Ćirković-Veličković, Tanja; Krstić-Ristivojević, Maja; Ionita, Valentin; Iovu, Horia; Matei, Ecaterina

(Elsevier Science Bv, Amsterdam, 2013)

TY  - JOUR
AU  - Covaliu, Cristina Ileana
AU  - Paraschiv, Gigel
AU  - Biris, Sorin-Stefan
AU  - Jitaru, Ioana
AU  - Vasile, Eugeniu
AU  - Diamandescu, Lucian
AU  - Ćirković-Veličković, Tanja
AU  - Krstić-Ristivojević, Maja
AU  - Ionita, Valentin
AU  - Iovu, Horia
AU  - Matei, Ecaterina
PY  - 2013
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1421
AB  - This paper deals with the synthesis of two nanohybrid materials based on maghemite (gamma-Fe2O3) and poly-DL-alanine using a two-step procedure consisting of maghemite nanoparticles synthesis by microemulsion method and nanohybrids obtaining by coating of maghemite nanoparticles with poly-DL-alanine biopolymer in two different molar ratios (H1:5 and H1: 15). The maghemite and their corresponding nanohybrids were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, X-ray photoemission spectroscopy, Mossbauer spectroscopy, Transmission electron microscopy, High resolution transmission electron microscopy with selected area electron diffraction and Atomic absorption spectroscopy. The two nanohybrids under the investigation have the average particle sizes of 22 nm and 23 nm. The Fourier transform infrared spectroscopy spectra and X-ray photoemission spectroscopy data indicate the existence of some interactions between the maghemite nanoparticles and poly-DL-alanine shell. The saturation magnetization values for maghemite and the two nanohybrids determined by a Vibrating Sample Magnetometer correspond to a typical superparamagnetic behavior suitable for applying in biomedical field. Also, with respect of biomedical application the biological activity of maghemite and its corresponding nanohybrids was investigated on healthy human cells (PBMC) and cancerous cells (HeLa). Furthermore, in order to support the multifunctionality of the gamma-Fe2O3 sample and nanohybrids we also investigated their wastewater treatment properties by measuring the removal efficiency of heavy metal Cd (II) ions.
PB  - Elsevier Science Bv, Amsterdam
T2  - Applied Surface Science
T1  - Maghemite and poly-DL-alanine based core-shell multifunctional nanohybrids for environmental protection and biomedicine applications
VL  - 285
SP  - 86
EP  - 95
DO  - 10.1016/j.apsusc.2013.08.059
ER  - 
@article{
author = "Covaliu, Cristina Ileana and Paraschiv, Gigel and Biris, Sorin-Stefan and Jitaru, Ioana and Vasile, Eugeniu and Diamandescu, Lucian and Ćirković-Veličković, Tanja and Krstić-Ristivojević, Maja and Ionita, Valentin and Iovu, Horia and Matei, Ecaterina",
year = "2013",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1421",
abstract = "This paper deals with the synthesis of two nanohybrid materials based on maghemite (gamma-Fe2O3) and poly-DL-alanine using a two-step procedure consisting of maghemite nanoparticles synthesis by microemulsion method and nanohybrids obtaining by coating of maghemite nanoparticles with poly-DL-alanine biopolymer in two different molar ratios (H1:5 and H1: 15). The maghemite and their corresponding nanohybrids were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, X-ray photoemission spectroscopy, Mossbauer spectroscopy, Transmission electron microscopy, High resolution transmission electron microscopy with selected area electron diffraction and Atomic absorption spectroscopy. The two nanohybrids under the investigation have the average particle sizes of 22 nm and 23 nm. The Fourier transform infrared spectroscopy spectra and X-ray photoemission spectroscopy data indicate the existence of some interactions between the maghemite nanoparticles and poly-DL-alanine shell. The saturation magnetization values for maghemite and the two nanohybrids determined by a Vibrating Sample Magnetometer correspond to a typical superparamagnetic behavior suitable for applying in biomedical field. Also, with respect of biomedical application the biological activity of maghemite and its corresponding nanohybrids was investigated on healthy human cells (PBMC) and cancerous cells (HeLa). Furthermore, in order to support the multifunctionality of the gamma-Fe2O3 sample and nanohybrids we also investigated their wastewater treatment properties by measuring the removal efficiency of heavy metal Cd (II) ions.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Applied Surface Science",
title = "Maghemite and poly-DL-alanine based core-shell multifunctional nanohybrids for environmental protection and biomedicine applications",
volume = "285",
pages = "86-95",
doi = "10.1016/j.apsusc.2013.08.059"
}
Covaliu, C. I., Paraschiv, G., Biris, S., Jitaru, I., Vasile, E., Diamandescu, L., Ćirković-Veličković, T., Krstić-Ristivojević, M., Ionita, V., Iovu, H.,& Matei, E. (2013). Maghemite and poly-DL-alanine based core-shell multifunctional nanohybrids for environmental protection and biomedicine applications.
Applied Surface Science
Elsevier Science Bv, Amsterdam., 285, 86-95.
https://doi.org/10.1016/j.apsusc.2013.08.059
Covaliu CI, Paraschiv G, Biris S, Jitaru I, Vasile E, Diamandescu L, Ćirković-Veličković T, Krstić-Ristivojević M, Ionita V, Iovu H, Matei E. Maghemite and poly-DL-alanine based core-shell multifunctional nanohybrids for environmental protection and biomedicine applications. Applied Surface Science. 2013;285:86-95
Covaliu Cristina Ileana, Paraschiv Gigel, Biris Sorin-Stefan, Jitaru Ioana, Vasile Eugeniu, Diamandescu Lucian, Ćirković-Veličković Tanja, Krstić-Ristivojević Maja, Ionita Valentin, Iovu Horia, Matei Ecaterina, "Maghemite and poly-DL-alanine based core-shell multifunctional nanohybrids for environmental protection and biomedicine applications" Applied Surface Science, 285 (2013):86-95,
https://doi.org/10.1016/j.apsusc.2013.08.059 .
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Two complexes of Co(II) and Pd(II) formed in reaction with a mono-oxazoline derivative. Spectroscopic characterization and cytotoxic evaluation

Alexandru, Maria-Gabriela; Ćirković-Veličković, Tanja; Krstić-Ristivojević, Maja; Hrubaru, Madalina-Marina; Draghici, Constantin

(Elsevier Science Bv, Amsterdam, 2013)

TY  - JOUR
AU  - Alexandru, Maria-Gabriela
AU  - Ćirković-Veličković, Tanja
AU  - Krstić-Ristivojević, Maja
AU  - Hrubaru, Madalina-Marina
AU  - Draghici, Constantin
PY  - 2013
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1356
AB  - Two coordination compounds obtained from 2-(4-ethoxyphenyl)-4,5-dihydro-1H-oxazole, L, [CoCl2(L)(2)] (1) and [OH-CH2-CH2-NH3](2)center dot[PdCl4] (2) were synthesized and characterized through elemental analysis, spectroscopic methods (FTIR, UV-Vis, H-1 NMR) and X-ray diffraction. Cytotoxicity tests on PBMC and HeLa cells were performed in order to evaluate the potential applications of these compounds in medicine. Also, compound (2) exhibits ligand assisted luminescent properties that were determined in solid state.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Molecular Structure
T1  - Two complexes of Co(II) and Pd(II) formed in reaction with a mono-oxazoline derivative. Spectroscopic characterization and cytotoxic evaluation
VL  - 1041
SP  - 55
EP  - 60
DO  - 10.1016/j.molstruc.2013.02.043
ER  - 
@article{
author = "Alexandru, Maria-Gabriela and Ćirković-Veličković, Tanja and Krstić-Ristivojević, Maja and Hrubaru, Madalina-Marina and Draghici, Constantin",
year = "2013",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1356",
abstract = "Two coordination compounds obtained from 2-(4-ethoxyphenyl)-4,5-dihydro-1H-oxazole, L, [CoCl2(L)(2)] (1) and [OH-CH2-CH2-NH3](2)center dot[PdCl4] (2) were synthesized and characterized through elemental analysis, spectroscopic methods (FTIR, UV-Vis, H-1 NMR) and X-ray diffraction. Cytotoxicity tests on PBMC and HeLa cells were performed in order to evaluate the potential applications of these compounds in medicine. Also, compound (2) exhibits ligand assisted luminescent properties that were determined in solid state.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Molecular Structure",
title = "Two complexes of Co(II) and Pd(II) formed in reaction with a mono-oxazoline derivative. Spectroscopic characterization and cytotoxic evaluation",
volume = "1041",
pages = "55-60",
doi = "10.1016/j.molstruc.2013.02.043"
}
Alexandru, M., Ćirković-Veličković, T., Krstić-Ristivojević, M., Hrubaru, M.,& Draghici, C. (2013). Two complexes of Co(II) and Pd(II) formed in reaction with a mono-oxazoline derivative. Spectroscopic characterization and cytotoxic evaluation.
Journal of Molecular Structure
Elsevier Science Bv, Amsterdam., 1041, 55-60.
https://doi.org/10.1016/j.molstruc.2013.02.043
Alexandru M, Ćirković-Veličković T, Krstić-Ristivojević M, Hrubaru M, Draghici C. Two complexes of Co(II) and Pd(II) formed in reaction with a mono-oxazoline derivative. Spectroscopic characterization and cytotoxic evaluation. Journal of Molecular Structure. 2013;1041:55-60
Alexandru Maria-Gabriela, Ćirković-Veličković Tanja, Krstić-Ristivojević Maja, Hrubaru Madalina-Marina, Draghici Constantin, "Two complexes of Co(II) and Pd(II) formed in reaction with a mono-oxazoline derivative. Spectroscopic characterization and cytotoxic evaluation" Journal of Molecular Structure, 1041 (2013):55-60,
https://doi.org/10.1016/j.molstruc.2013.02.043 .
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