Duduk, Bojan

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orcid::0000-0002-8109-7400
  • Duduk, Bojan (2)
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Author's Bibliography

Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw

Grujić, Marica; Dojnov, Biljana; Potočnik, Ivana; Atanasova, Lea; Duduk, Bojan; Srebotnik, Ewald; Druzhinina, Irirna S.; Kubicek, Christian P.; Vujčić, Zoran

(Springer Link, 2019)

TY  - JOUR
AU  - Grujić, Marica
AU  - Dojnov, Biljana
AU  - Potočnik, Ivana
AU  - Atanasova, Lea
AU  - Duduk, Bojan
AU  - Srebotnik, Ewald
AU  - Druzhinina, Irirna S.
AU  - Kubicek, Christian P.
AU  - Vujčić, Zoran
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3768
AB  - Lignocellulosic plant biomass is the world’s most abundant carbon source and has consequently attracted attention as a renewable resource for production of biofuels and commodity chemicals that could replace fossil resources. Due to its recalcitrant nature, it must be pretreated by chemical, physical or biological means prior to hydrolysis, introducing additional costs. In this paper, we tested the hypothesis that fungi which thrive on lignocellulosic material (straw, bark or soil) would be efficient in degrading untreated lignocellulose. Wheat straw was used as a model. We developed a fast and simple screening method for cellulase producers and tested one hundred Trichoderma strains isolated from wheat straw. The most potent strain—UB483FTG2/ TUCIM 4455, was isolated from substrate used for mushroom cultivation and was identified as T. guizhouense. After optimization of growth medium, high cellulase activity was already achieved after 72 h of fermentation on raw wheat straw, while the model cellulase overproducing strain T. reesei QM 9414 took 170 h and reached only 45% of the cellulase activity secreted by T. guizhouense. Maximum production levels were 1.1 U/mL (measured with CMC as cellulase substrate) and 0.7 U/mL (β-glucosidase assay). The T. guizhouense cellulase cocktail hydrolyzed raw wheat straw within 35 h. Our study shows that screening for fungi that successfully compete for special substrates in nature will lead to the isolation of strains with qualitatively and quantitatively superior enzymes needed for their digestion which could be used for industrial purposes.
PB  - Springer Link
T2  - World Journal of Microbiology and Biotechnology
T1  - Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw
VL  - 35
IS  - 12
DO  - 10.1007/s11274-019-2774-y
ER  - 
@article{
author = "Grujić, Marica and Dojnov, Biljana and Potočnik, Ivana and Atanasova, Lea and Duduk, Bojan and Srebotnik, Ewald and Druzhinina, Irirna S. and Kubicek, Christian P. and Vujčić, Zoran",
year = "2019",
abstract = "Lignocellulosic plant biomass is the world’s most abundant carbon source and has consequently attracted attention as a renewable resource for production of biofuels and commodity chemicals that could replace fossil resources. Due to its recalcitrant nature, it must be pretreated by chemical, physical or biological means prior to hydrolysis, introducing additional costs. In this paper, we tested the hypothesis that fungi which thrive on lignocellulosic material (straw, bark or soil) would be efficient in degrading untreated lignocellulose. Wheat straw was used as a model. We developed a fast and simple screening method for cellulase producers and tested one hundred Trichoderma strains isolated from wheat straw. The most potent strain—UB483FTG2/ TUCIM 4455, was isolated from substrate used for mushroom cultivation and was identified as T. guizhouense. After optimization of growth medium, high cellulase activity was already achieved after 72 h of fermentation on raw wheat straw, while the model cellulase overproducing strain T. reesei QM 9414 took 170 h and reached only 45% of the cellulase activity secreted by T. guizhouense. Maximum production levels were 1.1 U/mL (measured with CMC as cellulase substrate) and 0.7 U/mL (β-glucosidase assay). The T. guizhouense cellulase cocktail hydrolyzed raw wheat straw within 35 h. Our study shows that screening for fungi that successfully compete for special substrates in nature will lead to the isolation of strains with qualitatively and quantitatively superior enzymes needed for their digestion which could be used for industrial purposes.",
publisher = "Springer Link",
journal = "World Journal of Microbiology and Biotechnology",
title = "Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw",
volume = "35",
number = "12",
doi = "10.1007/s11274-019-2774-y"
}
Grujić, M., Dojnov, B., Potočnik, I., Atanasova, L., Duduk, B., Srebotnik, E., Druzhinina, I. S., Kubicek, C. P.,& Vujčić, Z.. (2019). Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw. in World Journal of Microbiology and Biotechnology
Springer Link., 35(12).
https://doi.org/10.1007/s11274-019-2774-y
Grujić M, Dojnov B, Potočnik I, Atanasova L, Duduk B, Srebotnik E, Druzhinina IS, Kubicek CP, Vujčić Z. Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw. in World Journal of Microbiology and Biotechnology. 2019;35(12).
doi:10.1007/s11274-019-2774-y .
Grujić, Marica, Dojnov, Biljana, Potočnik, Ivana, Atanasova, Lea, Duduk, Bojan, Srebotnik, Ewald, Druzhinina, Irirna S., Kubicek, Christian P., Vujčić, Zoran, "Superior cellulolytic activity of Trichoderma guizhouense on raw wheat straw" in World Journal of Microbiology and Biotechnology, 35, no. 12 (2019),
https://doi.org/10.1007/s11274-019-2774-y . .
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Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation

Grujić, Marica; Dojnov, Biljana; Potočnik, Ivana; Duduk, Bojan; Vujčić, Zoran

(Elsevier Sci Ltd, Oxford, 2015)

TY  - JOUR
AU  - Grujić, Marica
AU  - Dojnov, Biljana
AU  - Potočnik, Ivana
AU  - Duduk, Bojan
AU  - Vujčić, Zoran
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1974
AB  - Mushroom production is the biggest solid state fermentation industry in the world. Disposal and storage of spent mushroom compost (SMC) that remains after mushroom harvest poses a big economic and environmental problem. Production of industrially important hydrolytic enzymes by fungi on various agro-industrial wastes is a significant, open chapter in biotechnology. This paper proposes a novel application of SMC as substrate for cultivation of fungi in solid state fermentation (SSF) in order to obtain the enzymes cellulase, xylanase, amylase and beta-glucosidase. SMC can be used as a good substrate for cultivation of Trichoderma and Aspergillus without the addition of supplementary (nutritive) elements. Starting amount of SMC was reduced by 30% due to hydrolysis by a complex of cellulolytic enzymes. Material that is left behind is a more suitable fertilizer for horticulture. One fungal isolate was pointed out as a promising producer (Trichoderma atroviride isolate T42). It produced the greatest amount of total protein (0.204 mg mL(-1)), five isoforms of beta-glucosidase and the highest level (12 isoforms) of both endocellulase (0.76 U mL(-1)) and xylanase (2.31 U mL(-1)). The capacity of T42 to produce all examined enzymes in such a high number of isoforms demonstrates successful adaptation to new substrates.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Biodeterioration and Biodegradation
T1  - Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation
VL  - 104
SP  - 290
EP  - 298
DO  - 10.1016/j.ibiod.2015.04.029
UR  - Kon_2929
ER  - 
@article{
author = "Grujić, Marica and Dojnov, Biljana and Potočnik, Ivana and Duduk, Bojan and Vujčić, Zoran",
year = "2015",
abstract = "Mushroom production is the biggest solid state fermentation industry in the world. Disposal and storage of spent mushroom compost (SMC) that remains after mushroom harvest poses a big economic and environmental problem. Production of industrially important hydrolytic enzymes by fungi on various agro-industrial wastes is a significant, open chapter in biotechnology. This paper proposes a novel application of SMC as substrate for cultivation of fungi in solid state fermentation (SSF) in order to obtain the enzymes cellulase, xylanase, amylase and beta-glucosidase. SMC can be used as a good substrate for cultivation of Trichoderma and Aspergillus without the addition of supplementary (nutritive) elements. Starting amount of SMC was reduced by 30% due to hydrolysis by a complex of cellulolytic enzymes. Material that is left behind is a more suitable fertilizer for horticulture. One fungal isolate was pointed out as a promising producer (Trichoderma atroviride isolate T42). It produced the greatest amount of total protein (0.204 mg mL(-1)), five isoforms of beta-glucosidase and the highest level (12 isoforms) of both endocellulase (0.76 U mL(-1)) and xylanase (2.31 U mL(-1)). The capacity of T42 to produce all examined enzymes in such a high number of isoforms demonstrates successful adaptation to new substrates.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Biodeterioration and Biodegradation",
title = "Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation",
volume = "104",
pages = "290-298",
doi = "10.1016/j.ibiod.2015.04.029",
url = "Kon_2929"
}
Grujić, M., Dojnov, B., Potočnik, I., Duduk, B.,& Vujčić, Z.. (2015). Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation. in International Biodeterioration and Biodegradation
Elsevier Sci Ltd, Oxford., 104, 290-298.
https://doi.org/10.1016/j.ibiod.2015.04.029
Kon_2929
Grujić M, Dojnov B, Potočnik I, Duduk B, Vujčić Z. Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation. in International Biodeterioration and Biodegradation. 2015;104:290-298.
doi:10.1016/j.ibiod.2015.04.029
Kon_2929 .
Grujić, Marica, Dojnov, Biljana, Potočnik, Ivana, Duduk, Bojan, Vujčić, Zoran, "Spent mushroom compost as substrate for the production of industrially important hydrolytic enzymes by fungi Trichoderma spp. and Aspergillus niger in solid state fermentation" in International Biodeterioration and Biodegradation, 104 (2015):290-298,
https://doi.org/10.1016/j.ibiod.2015.04.029 .,
Kon_2929 .
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