TY  - DATA
AU  - Sentić, Milica
AU  - Virgilio, Francesca
AU  - Zanut, Alessandra
AU  - Manojlović, Dragan D.
AU  - Arbault, Stephane
AU  - Tormen, Massimo
AU  - Šojić, Nešo
AU  - Ugo, Paolo
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3566
PB  - Springer Heidelberg, Heidelberg
T2  - Analytical and Bioanalytical Chemistry
T1  - Supplementary data for the article: Sentic, M.; Virgilio, F.; Zanut, A.; Manojlovic, D.; Arbault, S.; Tormen, M.; Sojic, N.; Ugo, P. Microscopic Imaging and Tuning of Electrogenerated Chemiluminescence with Boron-Doped Diamond Nanoelectrode Arrays. Anal. Bioanal. Chem. 2016, 408 (25), 7085–7094. https://doi.org/10.1007/s00216-016-9504-1
DO  - 10.1007/s00216-016-9504-1
ER  - 

@misc{
author = "Sentić, Milica and Virgilio, Francesca and Zanut, Alessandra and Manojlović, Dragan D. and Arbault, Stephane and Tormen, Massimo and Šojić, Nešo and Ugo, Paolo",
year = "2016",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Analytical and Bioanalytical Chemistry",
title = "Supplementary data for the article: Sentic, M.; Virgilio, F.; Zanut, A.; Manojlovic, D.; Arbault, S.; Tormen, M.; Sojic, N.; Ugo, P. Microscopic Imaging and Tuning of Electrogenerated Chemiluminescence with Boron-Doped Diamond Nanoelectrode Arrays. Anal. Bioanal. Chem. 2016, 408 (25), 7085–7094. https://doi.org/10.1007/s00216-016-9504-1",
doi = "10.1007/s00216-016-9504-1"
}

Sentić, M., Virgilio, F., Zanut, A., Manojlović, D. D., Arbault, S., Tormen, M., Šojić, N.,& Ugo, P.. (2016). Supplementary data for the article: Sentic, M.; Virgilio, F.; Zanut, A.; Manojlovic, D.; Arbault, S.; Tormen, M.; Sojic, N.; Ugo, P. Microscopic Imaging and Tuning of Electrogenerated Chemiluminescence with Boron-Doped Diamond Nanoelectrode Arrays. Anal. Bioanal. Chem. 2016, 408 (25), 7085–7094. https://doi.org/10.1007/s00216-016-9504-1. in Analytical and Bioanalytical Chemistry
Springer Heidelberg, Heidelberg..
https://doi.org/10.1007/s00216-016-9504-1

Sentić M, Virgilio F, Zanut A, Manojlović DD, Arbault S, Tormen M, Šojić N, Ugo P. Supplementary data for the article: Sentic, M.; Virgilio, F.; Zanut, A.; Manojlovic, D.; Arbault, S.; Tormen, M.; Sojic, N.; Ugo, P. Microscopic Imaging and Tuning of Electrogenerated Chemiluminescence with Boron-Doped Diamond Nanoelectrode Arrays. Anal. Bioanal. Chem. 2016, 408 (25), 7085–7094. https://doi.org/10.1007/s00216-016-9504-1. in Analytical and Bioanalytical Chemistry. 2016;.
doi:10.1007/s00216-016-9504-1 .

Sentić, Milica, Virgilio, Francesca, Zanut, Alessandra, Manojlović, Dragan D., Arbault, Stephane, Tormen, Massimo, Šojić, Nešo, Ugo, Paolo, "Supplementary data for the article: Sentic, M.; Virgilio, F.; Zanut, A.; Manojlovic, D.; Arbault, S.; Tormen, M.; Sojic, N.; Ugo, P. Microscopic Imaging and Tuning of Electrogenerated Chemiluminescence with Boron-Doped Diamond Nanoelectrode Arrays. Anal. Bioanal. Chem. 2016, 408 (25), 7085–7094. https://doi.org/10.1007/s00216-016-9504-1" in Analytical and Bioanalytical Chemistry (2016),
https://doi.org/10.1007/s00216-016-9504-1 . .

TY  - JOUR
AU  - Sentić, Milica
AU  - Virgilio, Francesca
AU  - Zanut, Alessandra
AU  - Manojlović, Dragan D.
AU  - Arbault, Stephane
AU  - Tormen, Massimo
AU  - Šojić, Nešo
AU  - Ugo, Paolo
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2311
AB  - Nanoelectrode arrays (NEAs) are increasingly applied for a variety of electroanalytical applications; however, very few studies dealt with the use of NEAs as an electrochemical generator of electrogenerated chemiluminescence (ECL). In the present study, arrays of nanodisc and nanoband electrodes with different dimensions and inter-electrode distances were fabricated by e-beam lithography on a polycarbonate layer deposited on boron-doped diamond (BDD) substrates. In particular, NEAs with 16 different geometries were fabricated on the same BDD sample substrate obtaining a multiple nanoelectrode and ultramicroelectrode array platform (MNEAP). After electrochemical and morphological characterization, the MNEAP was used to capture simultaneously with a single image the characteristic behaviour of ECL emission from all the 16 arrays. Experiments were performed using Ru(bpy)(3) (2+) as the ECL luminophore and tri-n-propylamine (TPrA) as the co-reactant. With a relatively limited number of experiments, such an imaging procedure allowed to study the role that geometrical and mechanistic parameters play on ECL generation at NEAs. In particular, at high concentrations of TPrA, well-separated individual ECL spots or bands revealed an ECL signal which forms a pattern matching the nanofabricated structure. The analysis of the imaging data indicated that the thickness of the ECL-emitting zone at each nanoelectrode scales inversely with the co-reactant concentration, while significantly stronger ECL signals were detected for NEAs operating under overlap conditions.
PB  - Springer Heidelberg, Heidelberg
T2  - Analytical and Bioanalytical Chemistry
T1  - Microscopic imaging and tuning of electrogenerated chemiluminescence with boron-doped diamond nanoelectrode arrays
VL  - 408
IS  - 25
SP  - 7085
EP  - 7094
DO  - 10.1007/s00216-016-9504-1
ER  - 

@article{
author = "Sentić, Milica and Virgilio, Francesca and Zanut, Alessandra and Manojlović, Dragan D. and Arbault, Stephane and Tormen, Massimo and Šojić, Nešo and Ugo, Paolo",
year = "2016",
abstract = "Nanoelectrode arrays (NEAs) are increasingly applied for a variety of electroanalytical applications; however, very few studies dealt with the use of NEAs as an electrochemical generator of electrogenerated chemiluminescence (ECL). In the present study, arrays of nanodisc and nanoband electrodes with different dimensions and inter-electrode distances were fabricated by e-beam lithography on a polycarbonate layer deposited on boron-doped diamond (BDD) substrates. In particular, NEAs with 16 different geometries were fabricated on the same BDD sample substrate obtaining a multiple nanoelectrode and ultramicroelectrode array platform (MNEAP). After electrochemical and morphological characterization, the MNEAP was used to capture simultaneously with a single image the characteristic behaviour of ECL emission from all the 16 arrays. Experiments were performed using Ru(bpy)(3) (2+) as the ECL luminophore and tri-n-propylamine (TPrA) as the co-reactant. With a relatively limited number of experiments, such an imaging procedure allowed to study the role that geometrical and mechanistic parameters play on ECL generation at NEAs. In particular, at high concentrations of TPrA, well-separated individual ECL spots or bands revealed an ECL signal which forms a pattern matching the nanofabricated structure. The analysis of the imaging data indicated that the thickness of the ECL-emitting zone at each nanoelectrode scales inversely with the co-reactant concentration, while significantly stronger ECL signals were detected for NEAs operating under overlap conditions.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Analytical and Bioanalytical Chemistry",
title = "Microscopic imaging and tuning of electrogenerated chemiluminescence with boron-doped diamond nanoelectrode arrays",
volume = "408",
number = "25",
pages = "7085-7094",
doi = "10.1007/s00216-016-9504-1"
}

Sentić, M., Virgilio, F., Zanut, A., Manojlović, D. D., Arbault, S., Tormen, M., Šojić, N.,& Ugo, P.. (2016). Microscopic imaging and tuning of electrogenerated chemiluminescence with boron-doped diamond nanoelectrode arrays. in Analytical and Bioanalytical Chemistry
Springer Heidelberg, Heidelberg., 408(25), 7085-7094.
https://doi.org/10.1007/s00216-016-9504-1

Sentić M, Virgilio F, Zanut A, Manojlović DD, Arbault S, Tormen M, Šojić N, Ugo P. Microscopic imaging and tuning of electrogenerated chemiluminescence with boron-doped diamond nanoelectrode arrays. in Analytical and Bioanalytical Chemistry. 2016;408(25):7085-7094.
doi:10.1007/s00216-016-9504-1 .

Sentić, Milica, Virgilio, Francesca, Zanut, Alessandra, Manojlović, Dragan D., Arbault, Stephane, Tormen, Massimo, Šojić, Nešo, Ugo, Paolo, "Microscopic imaging and tuning of electrogenerated chemiluminescence with boron-doped diamond nanoelectrode arrays" in Analytical and Bioanalytical Chemistry, 408, no. 25 (2016):7085-7094,
https://doi.org/10.1007/s00216-016-9504-1 . .

TY  - DATA
AU  - Habtamu, Henok B.
AU  - Sentić, Milica
AU  - Silvestrini, Morena
AU  - De Leo, Luigina
AU  - Not, Tarcisio
AU  - Arbault, Stephane
AU  - Manojlović, Dragan D.
AU  - Šojić, Nešo
AU  - Ugo, Paolo
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3329
PB  - Amer Chemical Soc, Washington
T2  - Analytical Chemistry
T1  - Supplementary data for the article: Habtamu, H. B.; Sentic, M.; Silvestrini, M.; De Leo, L.; Not, T.; Arbault, S.; Manojlovic, D.; Sojic, N.; Ugo, P. A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles. Analytical Chemistry 2015, 87 (24), 12080–12087. https://doi.org/10.1021/acs.analchem.5b02801
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3329
ER  - 

@misc{
author = "Habtamu, Henok B. and Sentić, Milica and Silvestrini, Morena and De Leo, Luigina and Not, Tarcisio and Arbault, Stephane and Manojlović, Dragan D. and Šojić, Nešo and Ugo, Paolo",
year = "2015",
publisher = "Amer Chemical Soc, Washington",
journal = "Analytical Chemistry",
title = "Supplementary data for the article: Habtamu, H. B.; Sentic, M.; Silvestrini, M.; De Leo, L.; Not, T.; Arbault, S.; Manojlovic, D.; Sojic, N.; Ugo, P. A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles. Analytical Chemistry 2015, 87 (24), 12080–12087. https://doi.org/10.1021/acs.analchem.5b02801",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3329"
}

Habtamu, H. B., Sentić, M., Silvestrini, M., De Leo, L., Not, T., Arbault, S., Manojlović, D. D., Šojić, N.,& Ugo, P.. (2015). Supplementary data for the article: Habtamu, H. B.; Sentic, M.; Silvestrini, M.; De Leo, L.; Not, T.; Arbault, S.; Manojlovic, D.; Sojic, N.; Ugo, P. A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles. Analytical Chemistry 2015, 87 (24), 12080–12087. https://doi.org/10.1021/acs.analchem.5b02801. in Analytical Chemistry
Amer Chemical Soc, Washington..
https://hdl.handle.net/21.15107/rcub_cherry_3329

Habtamu HB, Sentić M, Silvestrini M, De Leo L, Not T, Arbault S, Manojlović DD, Šojić N, Ugo P. Supplementary data for the article: Habtamu, H. B.; Sentic, M.; Silvestrini, M.; De Leo, L.; Not, T.; Arbault, S.; Manojlovic, D.; Sojic, N.; Ugo, P. A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles. Analytical Chemistry 2015, 87 (24), 12080–12087. https://doi.org/10.1021/acs.analchem.5b02801. in Analytical Chemistry. 2015;.
https://hdl.handle.net/21.15107/rcub_cherry_3329 .

Habtamu, Henok B., Sentić, Milica, Silvestrini, Morena, De Leo, Luigina, Not, Tarcisio, Arbault, Stephane, Manojlović, Dragan D., Šojić, Nešo, Ugo, Paolo, "Supplementary data for the article: Habtamu, H. B.; Sentic, M.; Silvestrini, M.; De Leo, L.; Not, T.; Arbault, S.; Manojlovic, D.; Sojic, N.; Ugo, P. A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles. Analytical Chemistry 2015, 87 (24), 12080–12087. https://doi.org/10.1021/acs.analchem.5b02801" in Analytical Chemistry (2015),
https://hdl.handle.net/21.15107/rcub_cherry_3329 .

TY  - JOUR
AU  - Habtamu, Henok B.
AU  - Sentić, Milica
AU  - Silvestrini, Morena
AU  - De Leo, Luigina
AU  - Not, Tarcisio
AU  - Arbault, Stephane
AU  - Manojlović, Dragan D.
AU  - Šojić, Nešo
AU  - Ugo, Paolo
PY  - 2015
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2014
AB  - We report here the design of a novel immunosensor and its application for celiac disease diagnosis, based on an electrogenerated chemiluminescence (ECL) readout, using membrane-templated gold nanoelectrode ensembles (NEEs) as a detection platform. An original sensing strategy is presented by segregating spatially the initial electrochemical reaction and the location of the immobilized biomolecules where ECL is finally emitted. The recognition scaffold is the following: tissue transglutaminase (tTG) is immobilized as a capturing agent on the polycarbonate (PC) surface of the track-etched templating membrane. It captures the target tissue transglutaminase antibody (anti-tTG), and finally allows the immobilization of a streptavidin-modified ruthenium-based ECL label via reaction with a suitable biotinylated secondary antibody. The application of an oxidizing potential in a tri-n-propylamine (TPrA) solution generates an intense and sharp ECL signal, suitable for analytical purposes. Voltammetric and ECL analyses evidenced that the ruthenium complex is not oxidized directly at the surface of the nanoelectrodes; instead ECL is generated following the TPrA oxidation, which produces the TPrA(center dot+) and TPrA(center dot) radicals. With NEEs operating under total overlap diffusion conditions, high local fluxes of these reactive radicals are produced by the nanoelectrodes in the immediate vicinity of the ECL labels, so that they efficiently generate the ECL signal. The radicals can diffuse over short distances and react with the Ru(bpy)(3)(2+) label. In addition, the ECL emission is obtained by applying a potential of 0.88 V versus Ag/AgCl, which is about 0.3 V lower than when ECL is initiated by the electrochemical oxidation of Ru(bpy). The immunosensor provides ECL signals which scale with anti-tTG concentration with a linearity range between 1.5 ng.mL(-1) and 10 mu g.mL(-1) and a detection limit of 0.5 ng.mL(-1). The sensor is finally applied to the analysis of anti-tTG in human serum samples, showing to be suitable to discriminate between healthy and celiac patients.
PB  - Amer Chemical Soc, Washington
T2  - Analytical Chemistry
T1  - A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles
VL  - 87
IS  - 24
SP  - 12080
EP  - 12087
DO  - 10.1021/acs.analchem.5b02801
ER  - 

@article{
author = "Habtamu, Henok B. and Sentić, Milica and Silvestrini, Morena and De Leo, Luigina and Not, Tarcisio and Arbault, Stephane and Manojlović, Dragan D. and Šojić, Nešo and Ugo, Paolo",
year = "2015",
abstract = "We report here the design of a novel immunosensor and its application for celiac disease diagnosis, based on an electrogenerated chemiluminescence (ECL) readout, using membrane-templated gold nanoelectrode ensembles (NEEs) as a detection platform. An original sensing strategy is presented by segregating spatially the initial electrochemical reaction and the location of the immobilized biomolecules where ECL is finally emitted. The recognition scaffold is the following: tissue transglutaminase (tTG) is immobilized as a capturing agent on the polycarbonate (PC) surface of the track-etched templating membrane. It captures the target tissue transglutaminase antibody (anti-tTG), and finally allows the immobilization of a streptavidin-modified ruthenium-based ECL label via reaction with a suitable biotinylated secondary antibody. The application of an oxidizing potential in a tri-n-propylamine (TPrA) solution generates an intense and sharp ECL signal, suitable for analytical purposes. Voltammetric and ECL analyses evidenced that the ruthenium complex is not oxidized directly at the surface of the nanoelectrodes; instead ECL is generated following the TPrA oxidation, which produces the TPrA(center dot+) and TPrA(center dot) radicals. With NEEs operating under total overlap diffusion conditions, high local fluxes of these reactive radicals are produced by the nanoelectrodes in the immediate vicinity of the ECL labels, so that they efficiently generate the ECL signal. The radicals can diffuse over short distances and react with the Ru(bpy)(3)(2+) label. In addition, the ECL emission is obtained by applying a potential of 0.88 V versus Ag/AgCl, which is about 0.3 V lower than when ECL is initiated by the electrochemical oxidation of Ru(bpy). The immunosensor provides ECL signals which scale with anti-tTG concentration with a linearity range between 1.5 ng.mL(-1) and 10 mu g.mL(-1) and a detection limit of 0.5 ng.mL(-1). The sensor is finally applied to the analysis of anti-tTG in human serum samples, showing to be suitable to discriminate between healthy and celiac patients.",
publisher = "Amer Chemical Soc, Washington",
journal = "Analytical Chemistry",
title = "A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles",
volume = "87",
number = "24",
pages = "12080-12087",
doi = "10.1021/acs.analchem.5b02801"
}

Habtamu, H. B., Sentić, M., Silvestrini, M., De Leo, L., Not, T., Arbault, S., Manojlović, D. D., Šojić, N.,& Ugo, P.. (2015). A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles. in Analytical Chemistry
Amer Chemical Soc, Washington., 87(24), 12080-12087.
https://doi.org/10.1021/acs.analchem.5b02801

Habtamu HB, Sentić M, Silvestrini M, De Leo L, Not T, Arbault S, Manojlović DD, Šojić N, Ugo P. A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles. in Analytical Chemistry. 2015;87(24):12080-12087.
doi:10.1021/acs.analchem.5b02801 .

Habtamu, Henok B., Sentić, Milica, Silvestrini, Morena, De Leo, Luigina, Not, Tarcisio, Arbault, Stephane, Manojlović, Dragan D., Šojić, Nešo, Ugo, Paolo, "A Sensitive Electrochemiluminescence Immunosensor for Celiac Disease Diagnosis Based on Nanoelectrode Ensembles" in Analytical Chemistry, 87, no. 24 (2015):12080-12087,
https://doi.org/10.1021/acs.analchem.5b02801 . .