TY  - JOUR
AU  - Ranđelović, Dragana
AU  - Gajić, Gordana
AU  - Mutić, Jelena
AU  - Pavlović, Pavle
AU  - Mihailović, Nevena
AU  - Jovanović, Slobodan
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2332
AB  - Metalliferous mine wastes represent one of the major sources of environmental contamination from mining activities. Bor region (Serbia) is one of the largest copper mine basins in Europe where long-term mining caused severe environmental deterioration and created one of the most degraded locations in Serbia and Europe. At the spontaneously colonized metalliferous mine wastes in Bor, plant species Epilobium dodonaei dominates in the mine slopes and mine waste surfaces. Epilobium dodonaei has the status of endangered and protected species in parts of European range (i. e. plant is included in the Red lists of the countries in the Carpathian mountains region), primarily due to losses of natural gravel habitats. The main focus of this research was physico-chemical characterization of mine waste, assessment of phytoremediation potential and plant metabolic stress response of Epilobium dodonaei at the hot spot metalliferous mine site in order to evaluate the possibility for application of endangered species in ecological restoration. The Bor mine wastes are characterized by coarse soil texture, various pH (4.58-8.30), and elevated concentrations of arsenic (44.5-271 mg kg(-1)) and copper (311-2820 mg kg(-1)) that exceed the Serbian limiting threshold and remediation values. Oxidation of metal-sulfide minerals on waste surface leads to increased acidity, followed by elevated metal mobility of the mine spoil solution. Content of arsenic, copper, lead and zinc in roots of E. dodonaei was correlated with pseudo-total and EDTA-available concentrations in Bor mine spoils. Furthermore, the content of arsenic, copper, lead and zinc in roots (3.98 mg kg(-1), 140 mg kg(-1), 3.19 mg kg(-1), and 72.8 mg kg(-1), respectively) and shoots (4.69 mg kg(-1),57.7 mg kg(-1), 1.17 mg kg(-1), and 59.3 mg kg(-1), respectively) of E. dodonaei reflected the multi-metal pollution at the investigated site. Epilobium dodonaei largely retains copper, lead and zinc in roots than in shoots and has the potential for phytoremediation of mine wastes. Epilobium dodonaei at Bor mine spoil had a high content of malondialdehyde in roots and leaves as well as reduced chlorophylls and carotenoids content in leaves, indicating great oxidative stress. However, elevated arsenic and copper content could promote biosynthesis of antioxidants in roots and leaves of E. dodonaei at mine spoil. Creation of an endangered species habitat on mine waste rocks of the Bor mining area and similar sites of Carpatho-Balkan metallogenic province could successfully contribute to the preservation of E. dodonaei. Development of practical procedures for the selection and application of endangered plant species in reclamation should create stronger link between ecological restoration and conservation biology. Finally, the application of endangered plant species should take a more prominent role in the restoration process and ecosystem design. (C) 2016 Elsevier B. V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Ecological Engineering
T1  - Ecological potential of Epilobium dodonaei Vill. for restoration of metalliferous mine wastes
VL  - 95
SP  - 800
EP  - 810
DO  - 10.1016/j.ecoleng.2016.07.015
ER  - 

@article{
author = "Ranđelović, Dragana and Gajić, Gordana and Mutić, Jelena and Pavlović, Pavle and Mihailović, Nevena and Jovanović, Slobodan",
year = "2016",
abstract = "Metalliferous mine wastes represent one of the major sources of environmental contamination from mining activities. Bor region (Serbia) is one of the largest copper mine basins in Europe where long-term mining caused severe environmental deterioration and created one of the most degraded locations in Serbia and Europe. At the spontaneously colonized metalliferous mine wastes in Bor, plant species Epilobium dodonaei dominates in the mine slopes and mine waste surfaces. Epilobium dodonaei has the status of endangered and protected species in parts of European range (i. e. plant is included in the Red lists of the countries in the Carpathian mountains region), primarily due to losses of natural gravel habitats. The main focus of this research was physico-chemical characterization of mine waste, assessment of phytoremediation potential and plant metabolic stress response of Epilobium dodonaei at the hot spot metalliferous mine site in order to evaluate the possibility for application of endangered species in ecological restoration. The Bor mine wastes are characterized by coarse soil texture, various pH (4.58-8.30), and elevated concentrations of arsenic (44.5-271 mg kg(-1)) and copper (311-2820 mg kg(-1)) that exceed the Serbian limiting threshold and remediation values. Oxidation of metal-sulfide minerals on waste surface leads to increased acidity, followed by elevated metal mobility of the mine spoil solution. Content of arsenic, copper, lead and zinc in roots of E. dodonaei was correlated with pseudo-total and EDTA-available concentrations in Bor mine spoils. Furthermore, the content of arsenic, copper, lead and zinc in roots (3.98 mg kg(-1), 140 mg kg(-1), 3.19 mg kg(-1), and 72.8 mg kg(-1), respectively) and shoots (4.69 mg kg(-1),57.7 mg kg(-1), 1.17 mg kg(-1), and 59.3 mg kg(-1), respectively) of E. dodonaei reflected the multi-metal pollution at the investigated site. Epilobium dodonaei largely retains copper, lead and zinc in roots than in shoots and has the potential for phytoremediation of mine wastes. Epilobium dodonaei at Bor mine spoil had a high content of malondialdehyde in roots and leaves as well as reduced chlorophylls and carotenoids content in leaves, indicating great oxidative stress. However, elevated arsenic and copper content could promote biosynthesis of antioxidants in roots and leaves of E. dodonaei at mine spoil. Creation of an endangered species habitat on mine waste rocks of the Bor mining area and similar sites of Carpatho-Balkan metallogenic province could successfully contribute to the preservation of E. dodonaei. Development of practical procedures for the selection and application of endangered plant species in reclamation should create stronger link between ecological restoration and conservation biology. Finally, the application of endangered plant species should take a more prominent role in the restoration process and ecosystem design. (C) 2016 Elsevier B. V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Ecological Engineering",
title = "Ecological potential of Epilobium dodonaei Vill. for restoration of metalliferous mine wastes",
volume = "95",
pages = "800-810",
doi = "10.1016/j.ecoleng.2016.07.015"
}

Ranđelović, D., Gajić, G., Mutić, J., Pavlović, P., Mihailović, N.,& Jovanović, S.. (2016). Ecological potential of Epilobium dodonaei Vill. for restoration of metalliferous mine wastes. in Ecological Engineering
Elsevier Science Bv, Amsterdam., 95, 800-810.
https://doi.org/10.1016/j.ecoleng.2016.07.015

Ranđelović D, Gajić G, Mutić J, Pavlović P, Mihailović N, Jovanović S. Ecological potential of Epilobium dodonaei Vill. for restoration of metalliferous mine wastes. in Ecological Engineering. 2016;95:800-810.
doi:10.1016/j.ecoleng.2016.07.015 .

Ranđelović, Dragana, Gajić, Gordana, Mutić, Jelena, Pavlović, Pavle, Mihailović, Nevena, Jovanović, Slobodan, "Ecological potential of Epilobium dodonaei Vill. for restoration of metalliferous mine wastes" in Ecological Engineering, 95 (2016):800-810,
https://doi.org/10.1016/j.ecoleng.2016.07.015 . .

TY  - JOUR
AU  - Prodanović, Radivoje
AU  - Milosavić, Nenad
AU  - Jovanović, Slobodan
AU  - Prodanović, Olivera
AU  - Ćirković-Veličković, Tanja
AU  - Vujčić, Zoran
AU  - Jankov, Ratko M.
PY  - 2006
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/791
AB  - The activity of alpha-glucosidase from baker's yeast was determined in various concentrations of dioxan, tetrahydrofuran, tert-butanol, dimethylformamide, methanol and dimethylsulfoxide (DMSO). Higher activities were observed with sucrose than with nitrophenylglucoside as substrate in cosolvent mixtures. In 30% (v/v) DMSO, 25% of the activity obtained in pure water was detected, and in 30% (v/v) methanol 12.5% of the activity in pure water was detected, while in other cosolvents there was almost no activity under these conditions. alpha-glucosidase was immobilized onto a macroporous copolymer of ethylene glycol dimethacrylate and glycidyl methacrylate, poly(GMA-co-EGDMA), by the glutaraldehyde method. By immobilization, the half-life of the enzyme in 35% (v/v) methanol was increased from 6 to 60min and from 4 to 15min in 45% (v/v) DMSO. The activity of the immobilized enzyme in 30% (v/v) DMSO and 30% (v/v) methanol was 22% and 18% of the activity in pure water, respectively.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Biocatalysis and Biotransformation
T1  - Activity and stability of soluble and immobilized alpha-glucosidase from baker's yeast in cosolvent systems
VL  - 24
IS  - 3
SP  - 195
EP  - 200
DO  - 10.1080/10242420600655903
ER  - 

@article{
author = "Prodanović, Radivoje and Milosavić, Nenad and Jovanović, Slobodan and Prodanović, Olivera and Ćirković-Veličković, Tanja and Vujčić, Zoran and Jankov, Ratko M.",
year = "2006",
abstract = "The activity of alpha-glucosidase from baker's yeast was determined in various concentrations of dioxan, tetrahydrofuran, tert-butanol, dimethylformamide, methanol and dimethylsulfoxide (DMSO). Higher activities were observed with sucrose than with nitrophenylglucoside as substrate in cosolvent mixtures. In 30% (v/v) DMSO, 25% of the activity obtained in pure water was detected, and in 30% (v/v) methanol 12.5% of the activity in pure water was detected, while in other cosolvents there was almost no activity under these conditions. alpha-glucosidase was immobilized onto a macroporous copolymer of ethylene glycol dimethacrylate and glycidyl methacrylate, poly(GMA-co-EGDMA), by the glutaraldehyde method. By immobilization, the half-life of the enzyme in 35% (v/v) methanol was increased from 6 to 60min and from 4 to 15min in 45% (v/v) DMSO. The activity of the immobilized enzyme in 30% (v/v) DMSO and 30% (v/v) methanol was 22% and 18% of the activity in pure water, respectively.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Biocatalysis and Biotransformation",
title = "Activity and stability of soluble and immobilized alpha-glucosidase from baker's yeast in cosolvent systems",
volume = "24",
number = "3",
pages = "195-200",
doi = "10.1080/10242420600655903"
}

Prodanović, R., Milosavić, N., Jovanović, S., Prodanović, O., Ćirković-Veličković, T., Vujčić, Z.,& Jankov, R. M.. (2006). Activity and stability of soluble and immobilized alpha-glucosidase from baker's yeast in cosolvent systems. in Biocatalysis and Biotransformation
Taylor & Francis Ltd, Abingdon., 24(3), 195-200.
https://doi.org/10.1080/10242420600655903

Prodanović R, Milosavić N, Jovanović S, Prodanović O, Ćirković-Veličković T, Vujčić Z, Jankov RM. Activity and stability of soluble and immobilized alpha-glucosidase from baker's yeast in cosolvent systems. in Biocatalysis and Biotransformation. 2006;24(3):195-200.
doi:10.1080/10242420600655903 .

Prodanović, Radivoje, Milosavić, Nenad, Jovanović, Slobodan, Prodanović, Olivera, Ćirković-Veličković, Tanja, Vujčić, Zoran, Jankov, Ratko M., "Activity and stability of soluble and immobilized alpha-glucosidase from baker's yeast in cosolvent systems" in Biocatalysis and Biotransformation, 24, no. 3 (2006):195-200,
https://doi.org/10.1080/10242420600655903 . .