TY  - JOUR
AU  - Đurašinović, Tatjana
AU  - Lopandić, Zorana
AU  - Protić-Rosić, Isidora
AU  - Nešić, Andrijana
AU  - Trbojević-Ivić, Jovana
AU  - Jappe, Uta
AU  - Gavrović-Jankulović, Marija
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6338
AB  - Banana allergy is often associated with the pollen and latex allergies, which led us to the  hypothesis that some yet unidentified banana allergen could provide a basis of the latex-pollen-fruit syndrome. S-adenosyl-L-homocysteine hydrolase (SAHH) was recently identified in the literature as a novel plant allergen. This study aimed to assess the allergenic potential of the naturally occurring banana SAHH (nSAHH) and its recombinant homolog produced in E. coli (rSAHH). nSAHH showed IgE reactivity with a serum pool of twelve banana-allergic persons,
while rSAHH displayed IgE reactivity in ten out of the twelve tested patients. Five linear B-cell epitopes were identified on the rSAHH surface, exhibiting ≥ 90 % sequence homology with relevant plant SAHH allergens. Our findings have elucidated SAHH as a novel plant panallergen, underlying the cross-reactivity between plant derived food and respiratory allergens, confirming our initial hypothesis.
PB  - Elsevier
T2  - Food Chemistry
T1  - Identification of S-adenosyl-L-homocysteine hydrolase from banana fruit as a novel plant panallergen
VL  - 437
SP  - 137782
DO  - 10.1016/j.foodchem.2023.137782
ER  - 

@article{
author = "Đurašinović, Tatjana and Lopandić, Zorana and Protić-Rosić, Isidora and Nešić, Andrijana and Trbojević-Ivić, Jovana and Jappe, Uta and Gavrović-Jankulović, Marija",
year = "2024",
abstract = "Banana allergy is often associated with the pollen and latex allergies, which led us to the  hypothesis that some yet unidentified banana allergen could provide a basis of the latex-pollen-fruit syndrome. S-adenosyl-L-homocysteine hydrolase (SAHH) was recently identified in the literature as a novel plant allergen. This study aimed to assess the allergenic potential of the naturally occurring banana SAHH (nSAHH) and its recombinant homolog produced in E. coli (rSAHH). nSAHH showed IgE reactivity with a serum pool of twelve banana-allergic persons,
while rSAHH displayed IgE reactivity in ten out of the twelve tested patients. Five linear B-cell epitopes were identified on the rSAHH surface, exhibiting ≥ 90 % sequence homology with relevant plant SAHH allergens. Our findings have elucidated SAHH as a novel plant panallergen, underlying the cross-reactivity between plant derived food and respiratory allergens, confirming our initial hypothesis.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Identification of S-adenosyl-L-homocysteine hydrolase from banana fruit as a novel plant panallergen",
volume = "437",
pages = "137782",
doi = "10.1016/j.foodchem.2023.137782"
}

Đurašinović, T., Lopandić, Z., Protić-Rosić, I., Nešić, A., Trbojević-Ivić, J., Jappe, U.,& Gavrović-Jankulović, M.. (2024). Identification of S-adenosyl-L-homocysteine hydrolase from banana fruit as a novel plant panallergen. in Food Chemistry
Elsevier., 437, 137782.
https://doi.org/10.1016/j.foodchem.2023.137782

Đurašinović T, Lopandić Z, Protić-Rosić I, Nešić A, Trbojević-Ivić J, Jappe U, Gavrović-Jankulović M. Identification of S-adenosyl-L-homocysteine hydrolase from banana fruit as a novel plant panallergen. in Food Chemistry. 2024;437:137782.
doi:10.1016/j.foodchem.2023.137782 .

Đurašinović, Tatjana, Lopandić, Zorana, Protić-Rosić, Isidora, Nešić, Andrijana, Trbojević-Ivić, Jovana, Jappe, Uta, Gavrović-Jankulović, Marija, "Identification of S-adenosyl-L-homocysteine hydrolase from banana fruit as a novel plant panallergen" in Food Chemistry, 437 (2024):137782,
https://doi.org/10.1016/j.foodchem.2023.137782 . .

TY  - JOUR
AU  - Cavic, Milena
AU  - Nešić, Andrijana N.
AU  - Mirjacic Martinovic, Katarina
AU  - Vuletic, Ana
AU  - Besu Zizak, Irina
AU  - Tisma Miletic, Nevena
AU  - Krivokuca, Ana
AU  - Jankovic, Radmila
AU  - Gavrović-Jankulović, Marija
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6247
AB  - This study explored humoral and cellular responses to anti-SARS-CoV-2 BNT162b2 mRNA vaccine in breastfeeding women and naïve and seropositive individuals in the first six months after vaccination.Sixty-one volunteers vaccinated with two doses of the BNT162b2 mRNA vaccine were enrolled in the study. In-house developed ELISA was used for the quantification of SARS-CoV-2 RBD-specific antibodies. Cell surface marker expression and intracellular IFN-γ analysis were carried out by flow cytometry. The concentrations of IFN-γ, IL-6 and TNF were determined by ELISA. A significant rise in anti-RBD IgG antibody levels was observed 14 days after the first vaccine dose (p < 0.0001) in serum and milk. The expression of CD28 on CD4+ T cells was significantly higher compared to baseline (p < 0.05). There was a significant increase (p ≤ 0.05) in B cell lymphocyte subset after revaccination, and increased percentage of CD80+ B cells. The expression of IFN-γ in peripheral blood lymphocytes, CD3+ T cells and serum was significantly increased (p < 0.05). No significant difference in immune response was observed between breastfeeding women and other study participants. The anti-SARS-CoV-2 BNT162b2 mRNA vaccine-induced measurable and durable immune response in breastfeeding women and in naïve and previously infected individuals.
PB  - Nature Research
T2  - Scientific Reports
T1  - Detection of humoral and cellular immune response to anti-SARS-CoV-2 BNT162b2 vaccine in breastfeeding women and naïve and previously infected individuals
VL  - 13
IS  - 1
SP  - 6271
DO  - 10.1038/s41598-023-33516-1
ER  - 

@article{
author = "Cavic, Milena and Nešić, Andrijana N. and Mirjacic Martinovic, Katarina and Vuletic, Ana and Besu Zizak, Irina and Tisma Miletic, Nevena and Krivokuca, Ana and Jankovic, Radmila and Gavrović-Jankulović, Marija",
year = "2023",
abstract = "This study explored humoral and cellular responses to anti-SARS-CoV-2 BNT162b2 mRNA vaccine in breastfeeding women and naïve and seropositive individuals in the first six months after vaccination.Sixty-one volunteers vaccinated with two doses of the BNT162b2 mRNA vaccine were enrolled in the study. In-house developed ELISA was used for the quantification of SARS-CoV-2 RBD-specific antibodies. Cell surface marker expression and intracellular IFN-γ analysis were carried out by flow cytometry. The concentrations of IFN-γ, IL-6 and TNF were determined by ELISA. A significant rise in anti-RBD IgG antibody levels was observed 14 days after the first vaccine dose (p < 0.0001) in serum and milk. The expression of CD28 on CD4+ T cells was significantly higher compared to baseline (p < 0.05). There was a significant increase (p ≤ 0.05) in B cell lymphocyte subset after revaccination, and increased percentage of CD80+ B cells. The expression of IFN-γ in peripheral blood lymphocytes, CD3+ T cells and serum was significantly increased (p < 0.05). No significant difference in immune response was observed between breastfeeding women and other study participants. The anti-SARS-CoV-2 BNT162b2 mRNA vaccine-induced measurable and durable immune response in breastfeeding women and in naïve and previously infected individuals.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Detection of humoral and cellular immune response to anti-SARS-CoV-2 BNT162b2 vaccine in breastfeeding women and naïve and previously infected individuals",
volume = "13",
number = "1",
pages = "6271",
doi = "10.1038/s41598-023-33516-1"
}

Cavic, M., Nešić, A. N., Mirjacic Martinovic, K., Vuletic, A., Besu Zizak, I., Tisma Miletic, N., Krivokuca, A., Jankovic, R.,& Gavrović-Jankulović, M.. (2023). Detection of humoral and cellular immune response to anti-SARS-CoV-2 BNT162b2 vaccine in breastfeeding women and naïve and previously infected individuals. in Scientific Reports
Nature Research., 13(1), 6271.
https://doi.org/10.1038/s41598-023-33516-1

Cavic M, Nešić AN, Mirjacic Martinovic K, Vuletic A, Besu Zizak I, Tisma Miletic N, Krivokuca A, Jankovic R, Gavrović-Jankulović M. Detection of humoral and cellular immune response to anti-SARS-CoV-2 BNT162b2 vaccine in breastfeeding women and naïve and previously infected individuals. in Scientific Reports. 2023;13(1):6271.
doi:10.1038/s41598-023-33516-1 .

Cavic, Milena, Nešić, Andrijana N., Mirjacic Martinovic, Katarina, Vuletic, Ana, Besu Zizak, Irina, Tisma Miletic, Nevena, Krivokuca, Ana, Jankovic, Radmila, Gavrović-Jankulović, Marija, "Detection of humoral and cellular immune response to anti-SARS-CoV-2 BNT162b2 vaccine in breastfeeding women and naïve and previously infected individuals" in Scientific Reports, 13, no. 1 (2023):6271,
https://doi.org/10.1038/s41598-023-33516-1 . .

TY  - JOUR
AU  - Nešić, Andrijana N.
AU  - Stojsavljević, Aleksandar
AU  - Jagodić, Jovana
AU  - Čavić, Milena
AU  - Stefanović, Aleksandra
AU  - Manojlović, Dragan D.
AU  - Gavrović-Jankulović, Marija
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5636
AB  - Background: Although essential trace elements (ETEs) play pivotal roles in life-supporting biochemical processes, their function in innate and adaptive immunity has not been fully elucidated, particularly during immunization. Furthermore, the association between anti-SARS-CoV-2 specific IgG antibodies and ETE levels with vaccine responsiveness has not been investigated. Methods: The present study explored the status of ETEs (Mn, Cu, Zn, and Se) in sera of healthy women before and after vaccination with the anti-SARS-CoV-2 BNT162b2 mRNA vaccine in a follow-up period of six months. The main aim was to explore links between ETE levels and IgG antibodies produced against Spike glycoprotein's Receptor-Binding Domain (RBD). Results: A recombinant protein of SARS-CoV-2 comprising the receptor binding domain was successfully expressed in HEK-293 T cells. The purified protein was suitable for producing a sensitive antibody detection assay for human serum and monitored seropositivity, indicating a transient response with peak anti-SARS-CoV-2 IgG levels 2 months after vaccination. In parallel to increasing antibody titers, serum concentrations of Cu, Mn, and Se were not affected by vaccination, and concentrations remained relatively constant at the different sampling times during the 6-month observation period. Total serum Zn concentrations were slightly elevated when compared between the first and last sampling dates. Overall, no consistent effects of vaccination on any of the three trace elements analyzed in our study were observed. Conclusion: Vaccination of adult healthy female volunteers with an mRNA vaccine was not associated with consistent changes in serum trace element concentrations over a six-month observation period.
PB  - Elsevier
T2  - Journal of Trace Elements in Medicine and Biology
T1  - A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels
VL  - 74
SP  - 127079
DO  - 10.1016/j.jtemb.2022.127079
ER  - 

@article{
author = "Nešić, Andrijana N. and Stojsavljević, Aleksandar and Jagodić, Jovana and Čavić, Milena and Stefanović, Aleksandra and Manojlović, Dragan D. and Gavrović-Jankulović, Marija",
year = "2022",
abstract = "Background: Although essential trace elements (ETEs) play pivotal roles in life-supporting biochemical processes, their function in innate and adaptive immunity has not been fully elucidated, particularly during immunization. Furthermore, the association between anti-SARS-CoV-2 specific IgG antibodies and ETE levels with vaccine responsiveness has not been investigated. Methods: The present study explored the status of ETEs (Mn, Cu, Zn, and Se) in sera of healthy women before and after vaccination with the anti-SARS-CoV-2 BNT162b2 mRNA vaccine in a follow-up period of six months. The main aim was to explore links between ETE levels and IgG antibodies produced against Spike glycoprotein's Receptor-Binding Domain (RBD). Results: A recombinant protein of SARS-CoV-2 comprising the receptor binding domain was successfully expressed in HEK-293 T cells. The purified protein was suitable for producing a sensitive antibody detection assay for human serum and monitored seropositivity, indicating a transient response with peak anti-SARS-CoV-2 IgG levels 2 months after vaccination. In parallel to increasing antibody titers, serum concentrations of Cu, Mn, and Se were not affected by vaccination, and concentrations remained relatively constant at the different sampling times during the 6-month observation period. Total serum Zn concentrations were slightly elevated when compared between the first and last sampling dates. Overall, no consistent effects of vaccination on any of the three trace elements analyzed in our study were observed. Conclusion: Vaccination of adult healthy female volunteers with an mRNA vaccine was not associated with consistent changes in serum trace element concentrations over a six-month observation period.",
publisher = "Elsevier",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels",
volume = "74",
pages = "127079",
doi = "10.1016/j.jtemb.2022.127079"
}

Nešić, A. N., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D. D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology
Elsevier., 74, 127079.
https://doi.org/10.1016/j.jtemb.2022.127079

Nešić AN, Stojsavljević A, Jagodić J, Čavić M, Stefanović A, Manojlović DD, Gavrović-Jankulović M. A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology. 2022;74:127079.
doi:10.1016/j.jtemb.2022.127079 .

Nešić, Andrijana N., Stojsavljević, Aleksandar, Jagodić, Jovana, Čavić, Milena, Stefanović, Aleksandra, Manojlović, Dragan D., Gavrović-Jankulović, Marija, "A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels" in Journal of Trace Elements in Medicine and Biology, 74 (2022):127079,
https://doi.org/10.1016/j.jtemb.2022.127079 . .

TY  - DATA
AU  - Nešić, Andrijana N.
AU  - Stojsavljević, Aleksandar
AU  - Jagodić, Jovana
AU  - Čavić, Milena
AU  - Stefanović, Aleksandra
AU  - Manojlović, Dragan D.
AU  - Gavrović-Jankulović, Marija
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5658
AB  - Background: Although essential trace elements (ETEs) play pivotal roles in life-supporting biochemical processes, their function in innate and adaptive immunity has not been fully elucidated, particularly during immunization. Furthermore, the association between anti-SARS-CoV-2 specific IgG antibodies and ETE levels with vaccine responsiveness has not been investigated. Methods: The present study explored the status of ETEs (Mn, Cu, Zn, and Se) in sera of healthy women before and after vaccination with the anti-SARS-CoV-2 BNT162b2 mRNA vaccine in a follow-up period of six months. The main aim was to explore links between ETE levels and IgG antibodies produced against Spike glycoprotein's Receptor-Binding Domain (RBD). Results: A recombinant protein of SARS-CoV-2 comprising the receptor binding domain was successfully expressed in HEK-293 T cells. The purified protein was suitable for producing a sensitive antibody detection assay for human serum and monitored seropositivity, indicating a transient response with peak anti-SARS-CoV-2 IgG levels 2 months after vaccination. In parallel to increasing antibody titers, serum concentrations of Cu, Mn, and Se were not affected by vaccination, and concentrations remained relatively constant at the different sampling times during the 6-month observation period. Total serum Zn concentrations were slightly elevated when compared between the first and last sampling dates. Overall, no consistent effects of vaccination on any of the three trace elements analyzed in our study were observed. Conclusion: Vaccination of adult healthy female volunteers with an mRNA vaccine was not associated with consistent changes in serum trace element concentrations over a six-month observation period.
PB  - Elsevier
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Supplementary material for: Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology Elsevier., 74, 127079. https://doi.org/10.1016/j.jtemb.2022.127079
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5658
ER  - 

@misc{
author = "Nešić, Andrijana N. and Stojsavljević, Aleksandar and Jagodić, Jovana and Čavić, Milena and Stefanović, Aleksandra and Manojlović, Dragan D. and Gavrović-Jankulović, Marija",
year = "2022",
abstract = "Background: Although essential trace elements (ETEs) play pivotal roles in life-supporting biochemical processes, their function in innate and adaptive immunity has not been fully elucidated, particularly during immunization. Furthermore, the association between anti-SARS-CoV-2 specific IgG antibodies and ETE levels with vaccine responsiveness has not been investigated. Methods: The present study explored the status of ETEs (Mn, Cu, Zn, and Se) in sera of healthy women before and after vaccination with the anti-SARS-CoV-2 BNT162b2 mRNA vaccine in a follow-up period of six months. The main aim was to explore links between ETE levels and IgG antibodies produced against Spike glycoprotein's Receptor-Binding Domain (RBD). Results: A recombinant protein of SARS-CoV-2 comprising the receptor binding domain was successfully expressed in HEK-293 T cells. The purified protein was suitable for producing a sensitive antibody detection assay for human serum and monitored seropositivity, indicating a transient response with peak anti-SARS-CoV-2 IgG levels 2 months after vaccination. In parallel to increasing antibody titers, serum concentrations of Cu, Mn, and Se were not affected by vaccination, and concentrations remained relatively constant at the different sampling times during the 6-month observation period. Total serum Zn concentrations were slightly elevated when compared between the first and last sampling dates. Overall, no consistent effects of vaccination on any of the three trace elements analyzed in our study were observed. Conclusion: Vaccination of adult healthy female volunteers with an mRNA vaccine was not associated with consistent changes in serum trace element concentrations over a six-month observation period.",
publisher = "Elsevier",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Supplementary material for: Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology Elsevier., 74, 127079. https://doi.org/10.1016/j.jtemb.2022.127079",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5658"
}

Nešić, A. N., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D. D.,& Gavrović-Jankulović, M.. (2022). Supplementary material for: Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology Elsevier., 74, 127079. https://doi.org/10.1016/j.jtemb.2022.127079. in Journal of Trace Elements in Medicine and Biology
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_5658

Nešić AN, Stojsavljević A, Jagodić J, Čavić M, Stefanović A, Manojlović DD, Gavrović-Jankulović M. Supplementary material for: Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology Elsevier., 74, 127079. https://doi.org/10.1016/j.jtemb.2022.127079. in Journal of Trace Elements in Medicine and Biology. 2022;.
https://hdl.handle.net/21.15107/rcub_cherry_5658 .

Nešić, Andrijana N., Stojsavljević, Aleksandar, Jagodić, Jovana, Čavić, Milena, Stefanović, Aleksandra, Manojlović, Dragan D., Gavrović-Jankulović, Marija, "Supplementary material for: Nešić, A., Stojsavljević, A., Jagodić, J., Čavić, M., Stefanović, A., Manojlović, D.,& Gavrović-Jankulović, M.. (2022). A six-month study of anti-SARS-CoV-2 BNT162b2 mRNA vaccination: A comparative analysis of essential trace elements and anti-RBD IgG sera levels. in Journal of Trace Elements in Medicine and Biology Elsevier., 74, 127079. https://doi.org/10.1016/j.jtemb.2022.127079" in Journal of Trace Elements in Medicine and Biology (2022),
https://hdl.handle.net/21.15107/rcub_cherry_5658 .

TY  - JOUR
AU  - Knežević, Sara
AU  - Ognjanović, Miloš
AU  - Stanković, Vesna
AU  - Zlatanova, Milena
AU  - Nešić, Andrijana N.
AU  - Gavrović-Jankulović, Marija
AU  - Stanković, Dalibor
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5622
AB  - This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.
PB  - MDPI
T2  - Biosensors
T1  - La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells
VL  - 12
IS  - 9
SP  - 705
DO  - 10.3390/bios12090705
ER  - 

@article{
author = "Knežević, Sara and Ognjanović, Miloš and Stanković, Vesna and Zlatanova, Milena and Nešić, Andrijana N. and Gavrović-Jankulović, Marija and Stanković, Dalibor",
year = "2022",
abstract = "This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.",
publisher = "MDPI",
journal = "Biosensors",
title = "La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells",
volume = "12",
number = "9",
pages = "705",
doi = "10.3390/bios12090705"
}

Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A. N., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors
MDPI., 12(9), 705.
https://doi.org/10.3390/bios12090705

Knežević S, Ognjanović M, Stanković V, Zlatanova M, Nešić AN, Gavrović-Jankulović M, Stanković D. La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors. 2022;12(9):705.
doi:10.3390/bios12090705 .

Knežević, Sara, Ognjanović, Miloš, Stanković, Vesna, Zlatanova, Milena, Nešić, Andrijana N., Gavrović-Jankulović, Marija, Stanković, Dalibor, "La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells" in Biosensors, 12, no. 9 (2022):705,
https://doi.org/10.3390/bios12090705 . .

TY  - DATA
AU  - Knežević, Sara
AU  - Ognjanović, Miloš
AU  - Stanković, Vesna
AU  - Zlatanova, Milena
AU  - Nešić, Andrijana N.
AU  - Gavrović-Jankulović, Marija
AU  - Stanković, Dalibor
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5632
AB  - This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.
PB  - MDPI
T2  - Biosensors
T1  - Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705
VL  - 12
IS  - 9
SP  - 705
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5632
ER  - 

@misc{
author = "Knežević, Sara and Ognjanović, Miloš and Stanković, Vesna and Zlatanova, Milena and Nešić, Andrijana N. and Gavrović-Jankulović, Marija and Stanković, Dalibor",
year = "2022",
abstract = "This paper aims to develop an amperometric, non-enzymatic sensor for detecting and quantifying UA as an alert signal induced by allergens with protease activity in human cell lines (HEK293 and HeLa). Uric acid (UA) has been classified as a damage-associated molecular pattern (DAMP) molecule that serves a physiological purpose inside the cell, while outside the cell it can be an indicator of cell damage. Cell damage or stress can be caused by different health problems or by environmental irritants, such as allergens. We can act and prevent the events that generate stress by determining the extent to which cells are under stress. Amperometric calibration measurements were performed with a carbon paste electrode modified with La(OH)3@MWCNT, at the potential of 0.3 V. The calibration curve was constructed in a linear operating range from 0.67 μM to 121 μM UA. The proposed sensor displayed good reproducibility with an RSD of 3.65% calculated for five subsequent measurements, and a low detection limit of 64.28 nM, determined using the 3 S/m method. Interference studies and the real sample analysis of allergen-treated cell lines proved that the proposed sensing platform possesses excellent sensitivity, reproducibility, and stability. Therefore, it can potentially be used to evaluate stress factors in medical research and clinical practice.",
publisher = "MDPI",
journal = "Biosensors",
title = "Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705",
volume = "12",
number = "9",
pages = "705",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5632"
}

Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A. N., Gavrović-Jankulović, M.,& Stanković, D.. (2022). Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705. in Biosensors
MDPI., 12(9), 705.
https://hdl.handle.net/21.15107/rcub_cherry_5632

Knežević S, Ognjanović M, Stanković V, Zlatanova M, Nešić AN, Gavrović-Jankulović M, Stanković D. Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705. in Biosensors. 2022;12(9):705.
https://hdl.handle.net/21.15107/rcub_cherry_5632 .

Knežević, Sara, Ognjanović, Miloš, Stanković, Vesna, Zlatanova, Milena, Nešić, Andrijana N., Gavrović-Jankulović, Marija, Stanković, Dalibor, "Supplementary material for: Knežević, S., Ognjanović, M., Stanković, V., Zlatanova, M., Nešić, A., Gavrović-Jankulović, M.,& Stanković, D.. (2022). La(OH)3 Multi-Walled Carbon Nanotube/Carbon Paste-Based Sensing Approach for the Detection of Uric Acid—A Product of Environmentally Stressed Cells. in Biosensors MDPI., 12(9), 705. https://doi.org/10.3390/bios12090705" in Biosensors, 12, no. 9 (2022):705,
https://hdl.handle.net/21.15107/rcub_cherry_5632 .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Perović, Milan
AU  - Nešić, Andrijana N.
AU  - Miković, Željko
AU  - Manojlović, Dragan D.
PY  - 2022
UR  - https://link.springer.com/article/10.1007/s11356-022-20205-1
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5116
AB  - According to recent research, even low levels of environmental chemicals, particularly heavy metals, can considerably disrupt placental homeostasis. This review aims to explore the profile of non-essential trace metals in placental tissues across the globe and to specify trace metal(s) that can be candidates for impaired placental health. Accordingly, we conducted an extensive survey on relevant databases of peer-reviewed papers published in the last two decades. Among a considerable number of non-essential trace metals, arsenic (As), lead (Pb), cadmium (Cd), and mercury (Hg) were identified as the most detrimental to placental health. Comparative analysis showed remarkable differences in placental levels of these trace metals worldwide. Based on current data reported across the globe, a median (min–max) range from 0.55 to 15 ng/g for placental As levels could be deemed safe. The placental Cd and Pb levels were markedly higher in smokers than in non-smokers. Occupationally exposed pregnant women had several orders of magnitude higher Cd, Pb, and Hg levels in placental tissues than non-occupationally exposed women. Also, we concluded that even low-level exposure to As, Cd, Pb, and Hg could be deleterious to proper fetal development. This review implies the need to reduce exposure to non-essential trace metals to preserve placental health and prevent numerous poor pregnancy outcomes. Overall, the information presented is expected to help plan future fundamental and applied investigations on the placental toxicity of As, Cd, Pb, and Hg. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
PB  - SpringerLink
T2  - Environmental Science and Pollution Research
T1  - Levels of non-essential trace metals and their impact on placental health: a review
VL  - 29
SP  - 43662
EP  - 43674
DO  - 10.1007/s11356-022-20205-1
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Perović, Milan and Nešić, Andrijana N. and Miković, Željko and Manojlović, Dragan D.",
year = "2022",
abstract = "According to recent research, even low levels of environmental chemicals, particularly heavy metals, can considerably disrupt placental homeostasis. This review aims to explore the profile of non-essential trace metals in placental tissues across the globe and to specify trace metal(s) that can be candidates for impaired placental health. Accordingly, we conducted an extensive survey on relevant databases of peer-reviewed papers published in the last two decades. Among a considerable number of non-essential trace metals, arsenic (As), lead (Pb), cadmium (Cd), and mercury (Hg) were identified as the most detrimental to placental health. Comparative analysis showed remarkable differences in placental levels of these trace metals worldwide. Based on current data reported across the globe, a median (min–max) range from 0.55 to 15 ng/g for placental As levels could be deemed safe. The placental Cd and Pb levels were markedly higher in smokers than in non-smokers. Occupationally exposed pregnant women had several orders of magnitude higher Cd, Pb, and Hg levels in placental tissues than non-occupationally exposed women. Also, we concluded that even low-level exposure to As, Cd, Pb, and Hg could be deleterious to proper fetal development. This review implies the need to reduce exposure to non-essential trace metals to preserve placental health and prevent numerous poor pregnancy outcomes. Overall, the information presented is expected to help plan future fundamental and applied investigations on the placental toxicity of As, Cd, Pb, and Hg. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.",
publisher = "SpringerLink",
journal = "Environmental Science and Pollution Research",
title = "Levels of non-essential trace metals and their impact on placental health: a review",
volume = "29",
pages = "43662-43674",
doi = "10.1007/s11356-022-20205-1"
}

Stojsavljević, A., Perović, M., Nešić, A. N., Miković, Ž.,& Manojlović, D. D.. (2022). Levels of non-essential trace metals and their impact on placental health: a review. in Environmental Science and Pollution Research
SpringerLink., 29, 43662-43674.
https://doi.org/10.1007/s11356-022-20205-1

Stojsavljević A, Perović M, Nešić AN, Miković Ž, Manojlović DD. Levels of non-essential trace metals and their impact on placental health: a review. in Environmental Science and Pollution Research. 2022;29:43662-43674.
doi:10.1007/s11356-022-20205-1 .

Stojsavljević, Aleksandar, Perović, Milan, Nešić, Andrijana N., Miković, Željko, Manojlović, Dragan D., "Levels of non-essential trace metals and their impact on placental health: a review" in Environmental Science and Pollution Research, 29 (2022):43662-43674,
https://doi.org/10.1007/s11356-022-20205-1 . .

TY  - CONF
AU  - Đurašinović, Tatjana
AU  - Bazović, Vedrana
AU  - Trbojević-Ivić, Jovana
AU  - Nešić, Andrijana N.
AU  - Gavrović-Jankulović, Marija
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5165
PB  - Belgrade : Faculty of Chemistry
C3  - Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia
T1  - Screening of potential inhibitors of recombinant S-adenosyl-L-homocysteine hydrolase from banana
SP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5165
ER  - 

@conference{
author = "Đurašinović, Tatjana and Bazović, Vedrana and Trbojević-Ivić, Jovana and Nešić, Andrijana N. and Gavrović-Jankulović, Marija",
year = "2021",
publisher = "Belgrade : Faculty of Chemistry",
journal = "Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia",
title = "Screening of potential inhibitors of recombinant S-adenosyl-L-homocysteine hydrolase from banana",
pages = "50",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5165"
}

Đurašinović, T., Bazović, V., Trbojević-Ivić, J., Nešić, A. N.,& Gavrović-Jankulović, M.. (2021). Screening of potential inhibitors of recombinant S-adenosyl-L-homocysteine hydrolase from banana. in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia
Belgrade : Faculty of Chemistry., 50.
https://hdl.handle.net/21.15107/rcub_cherry_5165

Đurašinović T, Bazović V, Trbojević-Ivić J, Nešić AN, Gavrović-Jankulović M. Screening of potential inhibitors of recombinant S-adenosyl-L-homocysteine hydrolase from banana. in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia. 2021;:50.
https://hdl.handle.net/21.15107/rcub_cherry_5165 .

Đurašinović, Tatjana, Bazović, Vedrana, Trbojević-Ivić, Jovana, Nešić, Andrijana N., Gavrović-Jankulović, Marija, "Screening of potential inhibitors of recombinant S-adenosyl-L-homocysteine hydrolase from banana" in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia (2021):50,
https://hdl.handle.net/21.15107/rcub_cherry_5165 .

TY  - CONF
AU  - Pantović, Jelena
AU  - Trbojević-Ivić, Jovana
AU  - Nešić, Andrijana N.
AU  - Gavrović-Jankulović, Marija
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5166
PB  - Belgrade : Faculty of Chemistry
C3  - Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia
T1  - Expression, purification and characterization of recombinant L-phenylalanine dehydrogenase
SP  - 119
EP  - 120
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5166
ER  - 

@conference{
author = "Pantović, Jelena and Trbojević-Ivić, Jovana and Nešić, Andrijana N. and Gavrović-Jankulović, Marija",
year = "2021",
publisher = "Belgrade : Faculty of Chemistry",
journal = "Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia",
title = "Expression, purification and characterization of recombinant L-phenylalanine dehydrogenase",
pages = "119-120",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5166"
}

Pantović, J., Trbojević-Ivić, J., Nešić, A. N.,& Gavrović-Jankulović, M.. (2021). Expression, purification and characterization of recombinant L-phenylalanine dehydrogenase. in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia
Belgrade : Faculty of Chemistry., 119-120.
https://hdl.handle.net/21.15107/rcub_cherry_5166

Pantović J, Trbojević-Ivić J, Nešić AN, Gavrović-Jankulović M. Expression, purification and characterization of recombinant L-phenylalanine dehydrogenase. in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia. 2021;:119-120.
https://hdl.handle.net/21.15107/rcub_cherry_5166 .

Pantović, Jelena, Trbojević-Ivić, Jovana, Nešić, Andrijana N., Gavrović-Jankulović, Marija, "Expression, purification and characterization of recombinant L-phenylalanine dehydrogenase" in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia (2021):119-120,
https://hdl.handle.net/21.15107/rcub_cherry_5166 .

TY  - CONF
AU  - Zlatanova, Milena
AU  - Nešić, Andrijana N.
AU  - Trbojević-Ivić, Jovana
AU  - Gavrović-Jankulović, Marija
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5167
PB  - Belgrade : Faculty of Chemistry
C3  - Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia
T1  - Small molecules attenuate activation of the NF-kB signaling in epithelial cells by Act d 1 kiwifruit allergen
SP  - 186
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5167
ER  - 

@conference{
author = "Zlatanova, Milena and Nešić, Andrijana N. and Trbojević-Ivić, Jovana and Gavrović-Jankulović, Marija",
year = "2021",
publisher = "Belgrade : Faculty of Chemistry",
journal = "Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia",
title = "Small molecules attenuate activation of the NF-kB signaling in epithelial cells by Act d 1 kiwifruit allergen",
pages = "186",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5167"
}

Zlatanova, M., Nešić, A. N., Trbojević-Ivić, J.,& Gavrović-Jankulović, M.. (2021). Small molecules attenuate activation of the NF-kB signaling in epithelial cells by Act d 1 kiwifruit allergen. in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia
Belgrade : Faculty of Chemistry., 186.
https://hdl.handle.net/21.15107/rcub_cherry_5167

Zlatanova M, Nešić AN, Trbojević-Ivić J, Gavrović-Jankulović M. Small molecules attenuate activation of the NF-kB signaling in epithelial cells by Act d 1 kiwifruit allergen. in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia. 2021;:186.
https://hdl.handle.net/21.15107/rcub_cherry_5167 .

Zlatanova, Milena, Nešić, Andrijana N., Trbojević-Ivić, Jovana, Gavrović-Jankulović, Marija, "Small molecules attenuate activation of the NF-kB signaling in epithelial cells by Act d 1 kiwifruit allergen" in Proceedings of the Tenth Conference of the Serbian Biochemical Society, Biochemical Insight into Molecular Mechanicms, Serbia (2021):186,
https://hdl.handle.net/21.15107/rcub_cherry_5167 .

TY  - JOUR
AU  - Nešić, Andrijana N.
AU  - Čavić, Milena
AU  - Popović, Milica M.
AU  - Gavrović-Jankulović, Marija
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4549
AB  - Actinidin (Act d 1), a highly abundant cysteine protease from kiwifruit, is one of the major contributors to the development of kiwifruit allergy. Many studies have focused on the optimization of Act d 1 purification and its role in the development of food allergies. Testing on cell culture monolayers is a common step in the elucidation of food allergen sensitization. In the case of cysteine proteases, an additional activation step with l-cysteine is required before the testing. Hence, we aimed to evaluate whether l-cysteine already present in commonly used cell culture media would suffice for Act d 1 activation. Successfully activated Act d 1 (98.1% of proteolytic activity, as compared to l-cysteine activated Act d 1) was further tested in two commonly used 2D model systems (Caco-2 and HEK293 cells) to evaluate its role on the mRNA expression of cytokines involved in the innate immunity (IL-1β, IL-6, TNFα, TSLP). Furthermore, the contribution of Act d 1 in the promotion of inflammation through regulation of inducible nitric oxide synthase (iNOS) mRNA expression was also examined. These results demonstrate that activation of cysteine proteases can be achieved without previous enzyme incubation in l-cysteine -containing solution. Act d 1 incubated in cell culture medium was able to modulate gene expression of pro-inflammatory cytokines when tested on two model systems of the epithelial barrier. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.
PB  - Springer Nature
T2  - Molecular Biology Reports
T1  - A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing
VL  - 48
IS  - 5
SP  - 4065
EP  - 4072
DO  - 10.1007/s11033-021-06416-8
ER  - 

@article{
author = "Nešić, Andrijana N. and Čavić, Milena and Popović, Milica M. and Gavrović-Jankulović, Marija",
year = "2021",
abstract = "Actinidin (Act d 1), a highly abundant cysteine protease from kiwifruit, is one of the major contributors to the development of kiwifruit allergy. Many studies have focused on the optimization of Act d 1 purification and its role in the development of food allergies. Testing on cell culture monolayers is a common step in the elucidation of food allergen sensitization. In the case of cysteine proteases, an additional activation step with l-cysteine is required before the testing. Hence, we aimed to evaluate whether l-cysteine already present in commonly used cell culture media would suffice for Act d 1 activation. Successfully activated Act d 1 (98.1% of proteolytic activity, as compared to l-cysteine activated Act d 1) was further tested in two commonly used 2D model systems (Caco-2 and HEK293 cells) to evaluate its role on the mRNA expression of cytokines involved in the innate immunity (IL-1β, IL-6, TNFα, TSLP). Furthermore, the contribution of Act d 1 in the promotion of inflammation through regulation of inducible nitric oxide synthase (iNOS) mRNA expression was also examined. These results demonstrate that activation of cysteine proteases can be achieved without previous enzyme incubation in l-cysteine -containing solution. Act d 1 incubated in cell culture medium was able to modulate gene expression of pro-inflammatory cytokines when tested on two model systems of the epithelial barrier. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.",
publisher = "Springer Nature",
journal = "Molecular Biology Reports",
title = "A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing",
volume = "48",
number = "5",
pages = "4065-4072",
doi = "10.1007/s11033-021-06416-8"
}

Nešić, A. N., Čavić, M., Popović, M. M.,& Gavrović-Jankulović, M.. (2021). A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing. in Molecular Biology Reports
Springer Nature., 48(5), 4065-4072.
https://doi.org/10.1007/s11033-021-06416-8

Nešić AN, Čavić M, Popović MM, Gavrović-Jankulović M. A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing. in Molecular Biology Reports. 2021;48(5):4065-4072.
doi:10.1007/s11033-021-06416-8 .

Nešić, Andrijana N., Čavić, Milena, Popović, Milica M., Gavrović-Jankulović, Marija, "A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing" in Molecular Biology Reports, 48, no. 5 (2021):4065-4072,
https://doi.org/10.1007/s11033-021-06416-8 . .

TY  - JOUR
AU  - Milovanović, Vladimir
AU  - Minić, Rajna
AU  - Vakić, Jelena
AU  - Ivanović, Saša
AU  - Čupić, Vitomir
AU  - Borozan, Sunćica
AU  - Nešić, Andrijana N.
AU  - Živković, Irena
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0041010121000362
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4490
AB  - The MTT assay is routinely used to detect the activity of living cells. While working with Vipera ammodytes venom we detected the reduction of MTT without the presence of cells, in a concentration-dependent manner. By combining non-reducing PAGE, L-amino acid oxidase (LAAO) assays, and standard MTT assays, we established and confirmed that venom MTT reduction is catalyzed by only one enzyme, the LAAO. Even though it was previously known that the dimeric and tetrameric forms of LAAO are active, we conclude that the enzyme is also active in the monomeric form. Our results have led to the definition of a new MTT assay in a microtiter plate for in vitro testing of svLAAO activity i.e. from the venom of the V. ammodytes snake. Potentially, this method can be used for testing hemorrhagic venoms of other snakes as well as the LAAO neutralization capability of appropriate antivenoms.
PB  - Elsevier
T2  - Toxicon
T2  - ToxiconToxicon
T1  - MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation
VL  - 192
SP  - 57
EP  - 65
DO  - 10.1016/j.toxicon.2021.01.012
ER  - 

@article{
author = "Milovanović, Vladimir and Minić, Rajna and Vakić, Jelena and Ivanović, Saša and Čupić, Vitomir and Borozan, Sunćica and Nešić, Andrijana N. and Živković, Irena",
year = "2021",
abstract = "The MTT assay is routinely used to detect the activity of living cells. While working with Vipera ammodytes venom we detected the reduction of MTT without the presence of cells, in a concentration-dependent manner. By combining non-reducing PAGE, L-amino acid oxidase (LAAO) assays, and standard MTT assays, we established and confirmed that venom MTT reduction is catalyzed by only one enzyme, the LAAO. Even though it was previously known that the dimeric and tetrameric forms of LAAO are active, we conclude that the enzyme is also active in the monomeric form. Our results have led to the definition of a new MTT assay in a microtiter plate for in vitro testing of svLAAO activity i.e. from the venom of the V. ammodytes snake. Potentially, this method can be used for testing hemorrhagic venoms of other snakes as well as the LAAO neutralization capability of appropriate antivenoms.",
publisher = "Elsevier",
journal = "Toxicon, ToxiconToxicon",
title = "MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation",
volume = "192",
pages = "57-65",
doi = "10.1016/j.toxicon.2021.01.012"
}

Milovanović, V., Minić, R., Vakić, J., Ivanović, S., Čupić, V., Borozan, S., Nešić, A. N.,& Živković, I.. (2021). MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation. in Toxicon
Elsevier., 192, 57-65.
https://doi.org/10.1016/j.toxicon.2021.01.012

Milovanović V, Minić R, Vakić J, Ivanović S, Čupić V, Borozan S, Nešić AN, Živković I. MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation. in Toxicon. 2021;192:57-65.
doi:10.1016/j.toxicon.2021.01.012 .

Milovanović, Vladimir, Minić, Rajna, Vakić, Jelena, Ivanović, Saša, Čupić, Vitomir, Borozan, Sunćica, Nešić, Andrijana N., Živković, Irena, "MTT based L-aminoacid oxidase activity test for determination of antivenom potency against Vipera ammodytes envenomation" in Toxicon, 192 (2021):57-65,
https://doi.org/10.1016/j.toxicon.2021.01.012 . .

TY  - JOUR
AU  - Protić-Rosić, Isidora
AU  - Nešić, Andrijana N.
AU  - Lukić, Ivana
AU  - Miljković, Radmila
AU  - Popović, Dragan M.
AU  - Atanasković-Marković, Marina
AU  - Stojanović, Marijana M.
AU  - Gavrović-Jankulović, Marija
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0161589021001905
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4604
AB  - Allergen-specific immunotherapy (AIT) is a desensitizing treatment for allergic diseases that corrects the underlined pathological immune response to innocuous protein antigens, called allergens. Recombinant allergens employed in the AIT allowed the production of well-defined formulations that possessed consistent quality but were often less efficient than natural allergen extracts. Combining recombinant allergens with an adjuvant or immunomodulatory agent could improve AIT efficacy. This study aimed to perform structural and functional characterization of newly designed recombinant chimera composed of the Bet v 1, the major birch pollen allergen, and Banana Lectin (BanLec), TLR2, and CD14 binding protein, for the application in AIT. rBet v 1-BanLec chimera was designed in silico and expressed as a soluble fraction in Escherichia coli. Purified rBet v 1-BanLec (33.4 kDa) retained BanLec-associated biological activity of carbohydrate-binding and preserved IgE reactive epitopes of Bet v 1. The chimera revealed secondary structures with predominant β sheets. The immunomodulatory capacity of rBet v 1-BanLec tested on macrophages showed changes in myeloperoxidase activity, reduced NO production, and significant alterations in the production of cytokines when compared to both rBanLec and rBet v 1. Comparing to rBet v 1, rBet v 1-BanLec was demonstrated to be more efficient promoter of IL-10 production as well as weaker inducer of NO production and secretion of pro-inflammatory cytokines TNFα, and IL-6. The ability of rBet v 1-BanLec to promote IL-10 in together with the preserved 3D structure of Bet v 1 part implies that the construct might exert a beneficial effect in the allergen-specific immunotherapy.
PB  - Elsevier
T2  - Molecular Immunology
T2  - Molecular ImmunologyMolecular Immunology
T1  - Recombinant Bet v 1-BanLec chimera modulates functional characteristics of peritoneal murine macrophages by promoting IL-10 secretion
VL  - 138
SP  - 58
EP  - 67
DO  - 10.1016/j.molimm.2021.06.015
ER  - 

@article{
author = "Protić-Rosić, Isidora and Nešić, Andrijana N. and Lukić, Ivana and Miljković, Radmila and Popović, Dragan M. and Atanasković-Marković, Marina and Stojanović, Marijana M. and Gavrović-Jankulović, Marija",
year = "2021",
abstract = "Allergen-specific immunotherapy (AIT) is a desensitizing treatment for allergic diseases that corrects the underlined pathological immune response to innocuous protein antigens, called allergens. Recombinant allergens employed in the AIT allowed the production of well-defined formulations that possessed consistent quality but were often less efficient than natural allergen extracts. Combining recombinant allergens with an adjuvant or immunomodulatory agent could improve AIT efficacy. This study aimed to perform structural and functional characterization of newly designed recombinant chimera composed of the Bet v 1, the major birch pollen allergen, and Banana Lectin (BanLec), TLR2, and CD14 binding protein, for the application in AIT. rBet v 1-BanLec chimera was designed in silico and expressed as a soluble fraction in Escherichia coli. Purified rBet v 1-BanLec (33.4 kDa) retained BanLec-associated biological activity of carbohydrate-binding and preserved IgE reactive epitopes of Bet v 1. The chimera revealed secondary structures with predominant β sheets. The immunomodulatory capacity of rBet v 1-BanLec tested on macrophages showed changes in myeloperoxidase activity, reduced NO production, and significant alterations in the production of cytokines when compared to both rBanLec and rBet v 1. Comparing to rBet v 1, rBet v 1-BanLec was demonstrated to be more efficient promoter of IL-10 production as well as weaker inducer of NO production and secretion of pro-inflammatory cytokines TNFα, and IL-6. The ability of rBet v 1-BanLec to promote IL-10 in together with the preserved 3D structure of Bet v 1 part implies that the construct might exert a beneficial effect in the allergen-specific immunotherapy.",
publisher = "Elsevier",
journal = "Molecular Immunology, Molecular ImmunologyMolecular Immunology",
title = "Recombinant Bet v 1-BanLec chimera modulates functional characteristics of peritoneal murine macrophages by promoting IL-10 secretion",
volume = "138",
pages = "58-67",
doi = "10.1016/j.molimm.2021.06.015"
}

Protić-Rosić, I., Nešić, A. N., Lukić, I., Miljković, R., Popović, D. M., Atanasković-Marković, M., Stojanović, M. M.,& Gavrović-Jankulović, M.. (2021). Recombinant Bet v 1-BanLec chimera modulates functional characteristics of peritoneal murine macrophages by promoting IL-10 secretion. in Molecular Immunology
Elsevier., 138, 58-67.
https://doi.org/10.1016/j.molimm.2021.06.015

Protić-Rosić I, Nešić AN, Lukić I, Miljković R, Popović DM, Atanasković-Marković M, Stojanović MM, Gavrović-Jankulović M. Recombinant Bet v 1-BanLec chimera modulates functional characteristics of peritoneal murine macrophages by promoting IL-10 secretion. in Molecular Immunology. 2021;138:58-67.
doi:10.1016/j.molimm.2021.06.015 .

Protić-Rosić, Isidora, Nešić, Andrijana N., Lukić, Ivana, Miljković, Radmila, Popović, Dragan M., Atanasković-Marković, Marina, Stojanović, Marijana M., Gavrović-Jankulović, Marija, "Recombinant Bet v 1-BanLec chimera modulates functional characteristics of peritoneal murine macrophages by promoting IL-10 secretion" in Molecular Immunology, 138 (2021):58-67,
https://doi.org/10.1016/j.molimm.2021.06.015 . .

TY  - JOUR
AU  - Nešić, Andrijana N.
AU  - Čavić, Milena
AU  - Popović, Milica M.
AU  - Zlatanova, Milena
AU  - Pieters, Raymond
AU  - Smit, Joost
AU  - Gavrović-Jankulović, Marija
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3799
AB  - Impairment of the intestinal barrier is one of the key events in the initiation of the sensitization process in food allergy. The aim of this study was to explore the effects of kiwifruit allergen Act d 1 on intestinal permeability and tight junction protein (TJP) gene expression in vivo and to explore its potential to activate the NF-ĸB signaling pathway and to regulate expression of epithelial pro-allergenic cytokines. Influences of Act d 1 on TJP gene expression and pro-allergenic cytokines in the mouse intestine was analyzed by qPCR upon allergen administration by oral gavage. The effect on the in vivo intestinal permeability was assessed in ELISA by measuring the translocation of β-lactoglobulin (BLG) into circulation. The capacity of Act d 1 to activate the NF-ĸB pathway was tested in HEK293 cells by fluorescent microscopy and flow cytometry. Administration of Actinidin (Act d 1) increased intestinal permeability to the BLG. This was accompanied by changes in gene expression of TJP mRNA and pro-allergenic cytokines IL-25, IL-33, and thymic stromal lymphopoietin (TSLP) compared to the control. Act d 1 reduced TEER of the HEK293 monolayer, was positive in an NF-ĸB-reporter HEK293 cell assay, and induced secretion of TSLP. These findings shed more light on the molecular events in the sensitization process of kiwifruit but possibly also of other protease food allergens.
PB  - MDPI
T2  - Biomolecules
T1  - The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines
VL  - 9
IS  - 12
SP  - 816
DO  - 10.3390/biom9120816
ER  - 

@article{
author = "Nešić, Andrijana N. and Čavić, Milena and Popović, Milica M. and Zlatanova, Milena and Pieters, Raymond and Smit, Joost and Gavrović-Jankulović, Marija",
year = "2019",
abstract = "Impairment of the intestinal barrier is one of the key events in the initiation of the sensitization process in food allergy. The aim of this study was to explore the effects of kiwifruit allergen Act d 1 on intestinal permeability and tight junction protein (TJP) gene expression in vivo and to explore its potential to activate the NF-ĸB signaling pathway and to regulate expression of epithelial pro-allergenic cytokines. Influences of Act d 1 on TJP gene expression and pro-allergenic cytokines in the mouse intestine was analyzed by qPCR upon allergen administration by oral gavage. The effect on the in vivo intestinal permeability was assessed in ELISA by measuring the translocation of β-lactoglobulin (BLG) into circulation. The capacity of Act d 1 to activate the NF-ĸB pathway was tested in HEK293 cells by fluorescent microscopy and flow cytometry. Administration of Actinidin (Act d 1) increased intestinal permeability to the BLG. This was accompanied by changes in gene expression of TJP mRNA and pro-allergenic cytokines IL-25, IL-33, and thymic stromal lymphopoietin (TSLP) compared to the control. Act d 1 reduced TEER of the HEK293 monolayer, was positive in an NF-ĸB-reporter HEK293 cell assay, and induced secretion of TSLP. These findings shed more light on the molecular events in the sensitization process of kiwifruit but possibly also of other protease food allergens.",
publisher = "MDPI",
journal = "Biomolecules",
title = "The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines",
volume = "9",
number = "12",
pages = "816",
doi = "10.3390/biom9120816"
}

Nešić, A. N., Čavić, M., Popović, M. M., Zlatanova, M., Pieters, R., Smit, J.,& Gavrović-Jankulović, M.. (2019). The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines. in Biomolecules
MDPI., 9(12), 816.
https://doi.org/10.3390/biom9120816

Nešić AN, Čavić M, Popović MM, Zlatanova M, Pieters R, Smit J, Gavrović-Jankulović M. The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines. in Biomolecules. 2019;9(12):816.
doi:10.3390/biom9120816 .

Nešić, Andrijana N., Čavić, Milena, Popović, Milica M., Zlatanova, Milena, Pieters, Raymond, Smit, Joost, Gavrović-Jankulović, Marija, "The kiwifruit allergen act d 1 activates NF-κB signaling and affects mRNA expression of TJ proteins and innate pro-allergenic cytokines" in Biomolecules, 9, no. 12 (2019):816,
https://doi.org/10.3390/biom9120816 . .

TY  - THES
AU  - Nešić, Andrijana N.
PY  - 2019
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7662
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:22831/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=23740425
UR  - https://nardus.mpn.gov.rs/handle/123456789/17606
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4382
AB  - Gastrointestinalna barijera izgrađena je od veoma kompleksne mreže ćelija, mukusa i drugih važnih komponenti imunog sistema. Epitelni sloj ćelija gastrointestinalnog trakta predstavlja važnu komponentu urođene imunosti koja na dinamičan način održava homeostazu. Međutim, narušavanje integriteta gastrointestinalne barijere može se nepovoljno odraziti na organizam. Takav poremećaj dovodi do razvoja alergije na hranu, koja predstavlja neprimeren odgovor ćelija imunog sistema na proteine hrane. Epitelne ćelije gastrointestinalnog trakta, kao posledica kontakta sa alergenom hrane, utiču na komponente imunog sistema (smeštene u sloju lamina propria) u pravcu razvoja Th2 ćelijskog odgovora i proizvodnje specifičnih IgE antitela.Alergije na hranu nastaju kao posledica narušavanja imunološkog odgovora označenog kao oralna toleranca na proteine hrane koji podrazumeva kako proizvodnju specifičnih regulatornih ćelija tako i sintezu određenih signalnih molekula. Alergijske reakcije izazivaju širok spektar kliničkih simptoma i mogu se manifestovati od vrlo blagih reakcija do fatalnih, kao što je anafilaktički šok.Alergeni hrane koji poseduju proteaznu aktivnost u gastrointestinalnom traktu mogu da dovedu do narušavanja integriteta intestinalne barijere i time izazovu neprimerenu reakciju imunog sistema. Osim što pokazuju otpornost na proteolizu i drastične uslove digestivnog trakta, proteaze iz hrane mogu da zadrže i imunogena svojstva. Alergija na kivi je veoma rasprostranjena i značajan je uzročnik alergija na voće. Glavni alergen ploda zelenog kivija, aktinidin (Act d 1), je cistein proteaza (EC 3.4.22.14) koja je ujedno najzastupljeniji i najvažniji alergen kivija, čineći preko 50 % ukupnih solubilnih proteina kivija.U ovoj disertaciji ispitivan je efekat aktinidina i posledice njegove proteazne aktivnosti na epitelne ćelije gastrointestinalnog trakta...
AB  - The gastrointestinal (GI) barrier consists of a complex network of cells, mucus and other important components of the immune system. The epithelial cell layer of the gastrointestinal tract (GIT) is an important component of innate immunity, which dynamically maintains homeostasis. Therefore, disruption of the integrity of the gastrointestinal barrier can adversely affect the body. Food allergy, an inappropriate response of immune cells to food proteins, is an example of homeostasis disruption. After contact with a food allergen, gastrointestinal epithelial cells stimulate immune cells of lamina propria to induce Th2 cell response and produce specific immunoglobulin E (IgE). Oral tolerance is a normal response of the immune system to food proteins, which includes the production of specific regulatory cells and signal molecules. Disruption of oral tolerance gives rise to food allergy with a wide array of clinical symptoms from mild to life-threatening anaphylaxis. Food allergens with protease activity can impair the integrity of the gastrointestinal barrier and thus cause an inappropriate response of the immune system. These allergens are resistant to proteolysis in the harsh environment of the digestive tract and can also be immunogenic. Allergy to kiwi is among the most common fruit allergies with worldwide prevalence. Actinidin (Act d 1), cysteine protease (EC 3.4.22.14) is the most abundant allergen of green kiwi-fruit. In this dissertation, the effect of Act d 1 proteolytic activity on gastrointestinal epithelial cells was investigated in vitro and in vivo. It is shown that the proteolytic activity of actinidin impairs the integrity of the gastrointestinal barrier in C57BL/6 mice. The same was confirmed in human intestinal epithelial Caco-2 cells. Actinidin hydrolyzes tight junction (TJ) proteins in situ (occludin, ZO 1, claudin 3 and E-cadherin) in Caco-2 cells and 2D organoids derived from murine intestinal epithelial cells. Additionally, active actinidin affects the expression of mRNA for tight junction proteins in vitro. In vivo study in mouse animal model confirmed the results from in vitro study...
PB  - Универзитет у Београду, Хемијски факултет
T2  - Универзитет у Београду
T1  - Efekti proteazne aktivnosti aktinidina na integritet ćelija intestinalnog epitela
UR  - https://hdl.handle.net/21.15107/rcub_nardus_17606
ER  - 

@phdthesis{
author = "Nešić, Andrijana N.",
year = "2019",
abstract = "Gastrointestinalna barijera izgrađena je od veoma kompleksne mreže ćelija, mukusa i drugih važnih komponenti imunog sistema. Epitelni sloj ćelija gastrointestinalnog trakta predstavlja važnu komponentu urođene imunosti koja na dinamičan način održava homeostazu. Međutim, narušavanje integriteta gastrointestinalne barijere može se nepovoljno odraziti na organizam. Takav poremećaj dovodi do razvoja alergije na hranu, koja predstavlja neprimeren odgovor ćelija imunog sistema na proteine hrane. Epitelne ćelije gastrointestinalnog trakta, kao posledica kontakta sa alergenom hrane, utiču na komponente imunog sistema (smeštene u sloju lamina propria) u pravcu razvoja Th2 ćelijskog odgovora i proizvodnje specifičnih IgE antitela.Alergije na hranu nastaju kao posledica narušavanja imunološkog odgovora označenog kao oralna toleranca na proteine hrane koji podrazumeva kako proizvodnju specifičnih regulatornih ćelija tako i sintezu određenih signalnih molekula. Alergijske reakcije izazivaju širok spektar kliničkih simptoma i mogu se manifestovati od vrlo blagih reakcija do fatalnih, kao što je anafilaktički šok.Alergeni hrane koji poseduju proteaznu aktivnost u gastrointestinalnom traktu mogu da dovedu do narušavanja integriteta intestinalne barijere i time izazovu neprimerenu reakciju imunog sistema. Osim što pokazuju otpornost na proteolizu i drastične uslove digestivnog trakta, proteaze iz hrane mogu da zadrže i imunogena svojstva. Alergija na kivi je veoma rasprostranjena i značajan je uzročnik alergija na voće. Glavni alergen ploda zelenog kivija, aktinidin (Act d 1), je cistein proteaza (EC 3.4.22.14) koja je ujedno najzastupljeniji i najvažniji alergen kivija, čineći preko 50 % ukupnih solubilnih proteina kivija.U ovoj disertaciji ispitivan je efekat aktinidina i posledice njegove proteazne aktivnosti na epitelne ćelije gastrointestinalnog trakta..., The gastrointestinal (GI) barrier consists of a complex network of cells, mucus and other important components of the immune system. The epithelial cell layer of the gastrointestinal tract (GIT) is an important component of innate immunity, which dynamically maintains homeostasis. Therefore, disruption of the integrity of the gastrointestinal barrier can adversely affect the body. Food allergy, an inappropriate response of immune cells to food proteins, is an example of homeostasis disruption. After contact with a food allergen, gastrointestinal epithelial cells stimulate immune cells of lamina propria to induce Th2 cell response and produce specific immunoglobulin E (IgE). Oral tolerance is a normal response of the immune system to food proteins, which includes the production of specific regulatory cells and signal molecules. Disruption of oral tolerance gives rise to food allergy with a wide array of clinical symptoms from mild to life-threatening anaphylaxis. Food allergens with protease activity can impair the integrity of the gastrointestinal barrier and thus cause an inappropriate response of the immune system. These allergens are resistant to proteolysis in the harsh environment of the digestive tract and can also be immunogenic. Allergy to kiwi is among the most common fruit allergies with worldwide prevalence. Actinidin (Act d 1), cysteine protease (EC 3.4.22.14) is the most abundant allergen of green kiwi-fruit. In this dissertation, the effect of Act d 1 proteolytic activity on gastrointestinal epithelial cells was investigated in vitro and in vivo. It is shown that the proteolytic activity of actinidin impairs the integrity of the gastrointestinal barrier in C57BL/6 mice. The same was confirmed in human intestinal epithelial Caco-2 cells. Actinidin hydrolyzes tight junction (TJ) proteins in situ (occludin, ZO 1, claudin 3 and E-cadherin) in Caco-2 cells and 2D organoids derived from murine intestinal epithelial cells. Additionally, active actinidin affects the expression of mRNA for tight junction proteins in vitro. In vivo study in mouse animal model confirmed the results from in vitro study...",
publisher = "Универзитет у Београду, Хемијски факултет",
journal = "Универзитет у Београду",
title = "Efekti proteazne aktivnosti aktinidina na integritet ćelija intestinalnog epitela",
url = "https://hdl.handle.net/21.15107/rcub_nardus_17606"
}

Nešić, A. N.. (2019). Efekti proteazne aktivnosti aktinidina na integritet ćelija intestinalnog epitela. in Универзитет у Београду
Универзитет у Београду, Хемијски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_17606

Nešić AN. Efekti proteazne aktivnosti aktinidina na integritet ćelija intestinalnog epitela. in Универзитет у Београду. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_17606 .

Nešić, Andrijana N., "Efekti proteazne aktivnosti aktinidina na integritet ćelija intestinalnog epitela" in Универзитет у Београду (2019),
https://hdl.handle.net/21.15107/rcub_nardus_17606 .

TY  - JOUR
AU  - Nešić, Andrijana N.
AU  - Stam, Annemarie
AU  - Čavić, Milena
AU  - Ten Klooster, Jean Paul
AU  - Pieters, Raymond
AU  - Smit, Joost
AU  - Gavrović-Jankulović, Marija
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3485
AB  - In this study, two intestinal models were employed to assess the modulatory potential of a major kiwifruit allergen on the innate immunity of epithelial cells. Effects of Act d 1 were analyzed in terms of gene expression and structural changes of tight junction (TJ) proteins, as well as up-regulation of pro-inflammatory cytokines in Caco-2 cells and, for the first time, in mouse-derived intestinal 2-dimensional (2D) organoids.

Biologically active Act d 1 induced up-regulation of TJ genes for CLDN-2, CLDN-3, CLDN-4, ZO-1, and on the protein level induced release of pro-inflammatory cytokines IL-1β, TNFα and IL-33 in both employed model systems. In 2D-organoids, active Act d 1 impaired the TJ protein networks of E-cadherin, claudin-3, and ZO-1.

2D-organoids generated from mouse intestine are a promising new model system for the assessment of allergen-induced intestinal cell responses and a useful tool for mitigation of risks associated with novel food proteins.
T2  - Journal of Functional Foods
T1  - Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model
VL  - 62
DO  - 10.1016/j.jff.2019.103556
ER  - 

@article{
author = "Nešić, Andrijana N. and Stam, Annemarie and Čavić, Milena and Ten Klooster, Jean Paul and Pieters, Raymond and Smit, Joost and Gavrović-Jankulović, Marija",
year = "2019",
abstract = "In this study, two intestinal models were employed to assess the modulatory potential of a major kiwifruit allergen on the innate immunity of epithelial cells. Effects of Act d 1 were analyzed in terms of gene expression and structural changes of tight junction (TJ) proteins, as well as up-regulation of pro-inflammatory cytokines in Caco-2 cells and, for the first time, in mouse-derived intestinal 2-dimensional (2D) organoids.

Biologically active Act d 1 induced up-regulation of TJ genes for CLDN-2, CLDN-3, CLDN-4, ZO-1, and on the protein level induced release of pro-inflammatory cytokines IL-1β, TNFα and IL-33 in both employed model systems. In 2D-organoids, active Act d 1 impaired the TJ protein networks of E-cadherin, claudin-3, and ZO-1.

2D-organoids generated from mouse intestine are a promising new model system for the assessment of allergen-induced intestinal cell responses and a useful tool for mitigation of risks associated with novel food proteins.",
journal = "Journal of Functional Foods",
title = "Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model",
volume = "62",
doi = "10.1016/j.jff.2019.103556"
}

Nešić, A. N., Stam, A., Čavić, M., Ten Klooster, J. P., Pieters, R., Smit, J.,& Gavrović-Jankulović, M.. (2019). Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model. in Journal of Functional Foods, 62.
https://doi.org/10.1016/j.jff.2019.103556

Nešić AN, Stam A, Čavić M, Ten Klooster JP, Pieters R, Smit J, Gavrović-Jankulović M. Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model. in Journal of Functional Foods. 2019;62.
doi:10.1016/j.jff.2019.103556 .

Nešić, Andrijana N., Stam, Annemarie, Čavić, Milena, Ten Klooster, Jean Paul, Pieters, Raymond, Smit, Joost, Gavrović-Jankulović, Marija, "Activation of epithelial cells by the major kiwifruit allergen Act d 1 in human and mouse-derived intestinal model" in Journal of Functional Foods, 62 (2019),
https://doi.org/10.1016/j.jff.2019.103556 . .

TY  - JOUR
AU  - Nikolić, Jasna
AU  - Nešić, Andrijana N.
AU  - Kull, Skadi
AU  - Schocker, Frauke
AU  - Jappe, Uta
AU  - Gavrović-Jankulović, Marija
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2117
AB  - Diagnostic reagents based on food allergen extracts often lack sufficient sensitivity. The introduction of well characterized food allergens in molecular allergy diagnosis has been recognized as valid approach to circumvent unstandardized allergen extracts. Banana fruit (Musa acuminate) is a well-established allergen source which besides six characterized allergens, contains unidentified IgE reactive proteins whose clinical relevance remains undefined. By employment of a combinatorial peptide ligand library (CPLL) methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis, a novel allergen from banana fruit was detected in banana as catalase. A recombinant homologue of natural catalase was produced, isolated and biochemically characterized. The recombinant protein showed IgE reactivity in 7 out of 13 tested patients with suspected allergy to banana in immunoblot. Novel banana fruit allergens should be added as components to allergen-microarrays for the diagnosis and the monitoring of banana allergy. Significance: By employment of CPLL methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis catalase from banana fruit is identified as a novel allergen, with proposed designation as Mus a 7. IgE reactive recombinant Mus a 7 was produced and should be included in a component-resolved allergy diagnosis.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Proteomics
T1  - Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana
VL  - 175
SP  - 87
EP  - 94
DO  - 10.1016/j.jprot.2018.01.007
ER  - 

@article{
author = "Nikolić, Jasna and Nešić, Andrijana N. and Kull, Skadi and Schocker, Frauke and Jappe, Uta and Gavrović-Jankulović, Marija",
year = "2018",
abstract = "Diagnostic reagents based on food allergen extracts often lack sufficient sensitivity. The introduction of well characterized food allergens in molecular allergy diagnosis has been recognized as valid approach to circumvent unstandardized allergen extracts. Banana fruit (Musa acuminate) is a well-established allergen source which besides six characterized allergens, contains unidentified IgE reactive proteins whose clinical relevance remains undefined. By employment of a combinatorial peptide ligand library (CPLL) methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis, a novel allergen from banana fruit was detected in banana as catalase. A recombinant homologue of natural catalase was produced, isolated and biochemically characterized. The recombinant protein showed IgE reactivity in 7 out of 13 tested patients with suspected allergy to banana in immunoblot. Novel banana fruit allergens should be added as components to allergen-microarrays for the diagnosis and the monitoring of banana allergy. Significance: By employment of CPLL methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis catalase from banana fruit is identified as a novel allergen, with proposed designation as Mus a 7. IgE reactive recombinant Mus a 7 was produced and should be included in a component-resolved allergy diagnosis.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Proteomics",
title = "Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana",
volume = "175",
pages = "87-94",
doi = "10.1016/j.jprot.2018.01.007"
}

Nikolić, J., Nešić, A. N., Kull, S., Schocker, F., Jappe, U.,& Gavrović-Jankulović, M.. (2018). Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana. in Journal of Proteomics
Elsevier Science Bv, Amsterdam., 175, 87-94.
https://doi.org/10.1016/j.jprot.2018.01.007

Nikolić J, Nešić AN, Kull S, Schocker F, Jappe U, Gavrović-Jankulović M. Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana. in Journal of Proteomics. 2018;175:87-94.
doi:10.1016/j.jprot.2018.01.007 .

Nikolić, Jasna, Nešić, Andrijana N., Kull, Skadi, Schocker, Frauke, Jappe, Uta, Gavrović-Jankulović, Marija, "Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana" in Journal of Proteomics, 175 (2018):87-94,
https://doi.org/10.1016/j.jprot.2018.01.007 . .

TY  - DATA
AU  - Nikolić, Jasna
AU  - Nešić, Andrijana N.
AU  - Kull, Skadi
AU  - Schocker, Frauke
AU  - Jappe, Uta
AU  - Gavrović-Jankulović, Marija
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3203
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Proteomics
T1  - Supplementary material for the article: Nikolić, J.; Nešić, A.; Kull, S.; Schocker, F.; Jappe, U.; Gavrović-Jankulović, M.  Employment of Proteomic and Immunological Based Methods for the Identification of  Catalase as Novel Allergen from Banana. Journal of Proteomics 2018, 175, 87–94.  https://doi.org/10.1016/j.jprot.2018.01.007
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3203
ER  - 

@misc{
author = "Nikolić, Jasna and Nešić, Andrijana N. and Kull, Skadi and Schocker, Frauke and Jappe, Uta and Gavrović-Jankulović, Marija",
year = "2018",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Proteomics",
title = "Supplementary material for the article: Nikolić, J.; Nešić, A.; Kull, S.; Schocker, F.; Jappe, U.; Gavrović-Jankulović, M.  Employment of Proteomic and Immunological Based Methods for the Identification of  Catalase as Novel Allergen from Banana. Journal of Proteomics 2018, 175, 87–94.  https://doi.org/10.1016/j.jprot.2018.01.007",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3203"
}

Nikolić, J., Nešić, A. N., Kull, S., Schocker, F., Jappe, U.,& Gavrović-Jankulović, M.. (2018). Supplementary material for the article: Nikolić, J.; Nešić, A.; Kull, S.; Schocker, F.; Jappe, U.; Gavrović-Jankulović, M.  Employment of Proteomic and Immunological Based Methods for the Identification of  Catalase as Novel Allergen from Banana. Journal of Proteomics 2018, 175, 87–94.  https://doi.org/10.1016/j.jprot.2018.01.007. in Journal of Proteomics
Elsevier Science Bv, Amsterdam..
https://hdl.handle.net/21.15107/rcub_cherry_3203

Nikolić J, Nešić AN, Kull S, Schocker F, Jappe U, Gavrović-Jankulović M. Supplementary material for the article: Nikolić, J.; Nešić, A.; Kull, S.; Schocker, F.; Jappe, U.; Gavrović-Jankulović, M.  Employment of Proteomic and Immunological Based Methods for the Identification of  Catalase as Novel Allergen from Banana. Journal of Proteomics 2018, 175, 87–94.  https://doi.org/10.1016/j.jprot.2018.01.007. in Journal of Proteomics. 2018;.
https://hdl.handle.net/21.15107/rcub_cherry_3203 .

Nikolić, Jasna, Nešić, Andrijana N., Kull, Skadi, Schocker, Frauke, Jappe, Uta, Gavrović-Jankulović, Marija, "Supplementary material for the article: Nikolić, J.; Nešić, A.; Kull, S.; Schocker, F.; Jappe, U.; Gavrović-Jankulović, M.  Employment of Proteomic and Immunological Based Methods for the Identification of  Catalase as Novel Allergen from Banana. Journal of Proteomics 2018, 175, 87–94.  https://doi.org/10.1016/j.jprot.2018.01.007" in Journal of Proteomics (2018),
https://hdl.handle.net/21.15107/rcub_cherry_3203 .

TY  - JOUR
AU  - Nikolić, Jasna
AU  - Nešić, Andrijana N.
AU  - Čavić, Milena
AU  - Đorđević, Neda O.
AU  - Anđelković, Uroš
AU  - Atanasković-Marković, Marina
AU  - Drakulić, Branko J.
AU  - Gavrović-Jankulović, Marija
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2384
AB  - Background: Protein oxidation can occur as a consequence of lipid peroxidation during food processing. The aim of this work was to explore the effect of malondialdehyde (MDA) modification of ovalbumin (OVA) on its interaction with T84 intestinal cells. Methods: Molecular dynamics simulation was employed for the prediction of MDA modification in the OVA, while introduced structural changes were evaluated by measurement of carbonyl group content, fluorescence spectra, MS/MS analysis, and IgE reactivity. Effects of MDA modified OVA on T84 epithelial cells were analyzed by gene expression for pro-inflammatory cytokines and protein secretion. Results: Out of 9 predicted, five modified Lys residues were confirmed by MS/MS analysis: (51)TQINKVVR(58), (85)DILNQITKPNDVYSFSLASR(104), (111)YPILPEYLQCVKELYR(126), (187)AFKDEDTQAMPFR(99), (KIKVYLPR284)-K-277, and (IKVYLPR284)-I-278. The introduced MDA modifications influenced profile of IgE reactivity to OVA. Treatment of T84 epithelial cells with OVA and OVA modified with 1 mM MDA, induced up-regulation of pro-inflammatory cytokines (IL-1 beta,IL-25, IL-33, TSLP and TNF alpha), while OVA modification with 10 mM MDA induced down regulation of the cytokine expression profile, except for IL-1 beta. OVA and OVA modified with 1 mM MDA induced secretion of epithelial cells specific cytokine IL-33. Conclusions: This finding indicated that OVA and its MDA modified form have the potential to trigger the innate immunity by inducing up-regulation and secretion of pro-allergenic IL-33 in T84 intestinal epithelial cells. General significance: Interactions of ovalbumin and its MDA modified form with intestinal epithelial cells can induce a specific immunological priming necessary for the downstream activation of innate immunity. (C) 2016 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Biochimica et Biophysica Acta: General Subjects
T1  - Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells
VL  - 1861
IS  - 2
SP  - 126
EP  - 134
DO  - 10.1016/j.bbagen.2016.11.021
ER  - 

@article{
author = "Nikolić, Jasna and Nešić, Andrijana N. and Čavić, Milena and Đorđević, Neda O. and Anđelković, Uroš and Atanasković-Marković, Marina and Drakulić, Branko J. and Gavrović-Jankulović, Marija",
year = "2017",
abstract = "Background: Protein oxidation can occur as a consequence of lipid peroxidation during food processing. The aim of this work was to explore the effect of malondialdehyde (MDA) modification of ovalbumin (OVA) on its interaction with T84 intestinal cells. Methods: Molecular dynamics simulation was employed for the prediction of MDA modification in the OVA, while introduced structural changes were evaluated by measurement of carbonyl group content, fluorescence spectra, MS/MS analysis, and IgE reactivity. Effects of MDA modified OVA on T84 epithelial cells were analyzed by gene expression for pro-inflammatory cytokines and protein secretion. Results: Out of 9 predicted, five modified Lys residues were confirmed by MS/MS analysis: (51)TQINKVVR(58), (85)DILNQITKPNDVYSFSLASR(104), (111)YPILPEYLQCVKELYR(126), (187)AFKDEDTQAMPFR(99), (KIKVYLPR284)-K-277, and (IKVYLPR284)-I-278. The introduced MDA modifications influenced profile of IgE reactivity to OVA. Treatment of T84 epithelial cells with OVA and OVA modified with 1 mM MDA, induced up-regulation of pro-inflammatory cytokines (IL-1 beta,IL-25, IL-33, TSLP and TNF alpha), while OVA modification with 10 mM MDA induced down regulation of the cytokine expression profile, except for IL-1 beta. OVA and OVA modified with 1 mM MDA induced secretion of epithelial cells specific cytokine IL-33. Conclusions: This finding indicated that OVA and its MDA modified form have the potential to trigger the innate immunity by inducing up-regulation and secretion of pro-allergenic IL-33 in T84 intestinal epithelial cells. General significance: Interactions of ovalbumin and its MDA modified form with intestinal epithelial cells can induce a specific immunological priming necessary for the downstream activation of innate immunity. (C) 2016 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Biochimica et Biophysica Acta: General Subjects",
title = "Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells",
volume = "1861",
number = "2",
pages = "126-134",
doi = "10.1016/j.bbagen.2016.11.021"
}

Nikolić, J., Nešić, A. N., Čavić, M., Đorđević, N. O., Anđelković, U., Atanasković-Marković, M., Drakulić, B. J.,& Gavrović-Jankulović, M.. (2017). Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells. in Biochimica et Biophysica Acta: General Subjects
Elsevier Science Bv, Amsterdam., 1861(2), 126-134.
https://doi.org/10.1016/j.bbagen.2016.11.021

Nikolić J, Nešić AN, Čavić M, Đorđević NO, Anđelković U, Atanasković-Marković M, Drakulić BJ, Gavrović-Jankulović M. Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells. in Biochimica et Biophysica Acta: General Subjects. 2017;1861(2):126-134.
doi:10.1016/j.bbagen.2016.11.021 .

Nikolić, Jasna, Nešić, Andrijana N., Čavić, Milena, Đorđević, Neda O., Anđelković, Uroš, Atanasković-Marković, Marina, Drakulić, Branko J., Gavrović-Jankulović, Marija, "Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells" in Biochimica et Biophysica Acta: General Subjects, 1861, no. 2 (2017):126-134,
https://doi.org/10.1016/j.bbagen.2016.11.021 . .

TY  - CONF
AU  - Gavrović-Jankulović, Marija
AU  - Nešić, Andrijana N.
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2409
PB  - Wiley, Hoboken
C3  - FEBS Journal / Federation of European of Biochemical Societies
T1  - Evaluation of structural features of recombinant Mus a 5 allergen from Musa acuminata
VL  - 284
SP  - 215
EP  - 215
UR  - https://hdl.handle.net/21.15107/rcub_cherry_2409
ER  - 

@conference{
author = "Gavrović-Jankulović, Marija and Nešić, Andrijana N.",
year = "2017",
publisher = "Wiley, Hoboken",
journal = "FEBS Journal / Federation of European of Biochemical Societies",
title = "Evaluation of structural features of recombinant Mus a 5 allergen from Musa acuminata",
volume = "284",
pages = "215-215",
url = "https://hdl.handle.net/21.15107/rcub_cherry_2409"
}

Gavrović-Jankulović, M.,& Nešić, A. N.. (2017). Evaluation of structural features of recombinant Mus a 5 allergen from Musa acuminata. in FEBS Journal / Federation of European of Biochemical Societies
Wiley, Hoboken., 284, 215-215.
https://hdl.handle.net/21.15107/rcub_cherry_2409

Gavrović-Jankulović M, Nešić AN. Evaluation of structural features of recombinant Mus a 5 allergen from Musa acuminata. in FEBS Journal / Federation of European of Biochemical Societies. 2017;284:215-215.
https://hdl.handle.net/21.15107/rcub_cherry_2409 .

Gavrović-Jankulović, Marija, Nešić, Andrijana N., "Evaluation of structural features of recombinant Mus a 5 allergen from Musa acuminata" in FEBS Journal / Federation of European of Biochemical Societies, 284 (2017):215-215,
https://hdl.handle.net/21.15107/rcub_cherry_2409 .

TY  - JOUR
AU  - Nikolić, Jasna
AU  - Nešić, Andrijana N.
AU  - Čavić, Milena
AU  - Đorđević, Neda O.
AU  - Anđelković, Uroš
AU  - Atanasković-Marković, Marina
AU  - Drakulić, Branko J.
AU  - Gavrović-Jankulović, Marija
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2384
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2954
AB  - ackground: Protein oxidation can occur as a consequence of lipid peroxidation during food processing. The aim of this work was to explore the effect of malondialdehyde (MDA) modification of ovalbumin (OVA) on its interaction with T84 intestinal cells. Methods: Molecular dynamics simulation was employed for the prediction of MDA modification in the OVA, while introduced structural changes were evaluated by measurement of carbonyl group content, fluorescence spectra, MS/MS analysis, and IgE reactivity. Effects of MDA modified OVA on T84 epithelial cells were analyzed by gene expression for pro-inflammatory cytokines and protein secretion. Results: Out of 9 predicted, five modified Lys residues were confirmed by MS/MS analysis: (51)TQINKVVR(58), (85)DILNQITKPNDVYSFSLASR(104), (111)YPILPEYLQCVKELYR(126), (187)AFKDEDTQAMPFR(99), (KIKVYLPR284)-K-277, and (IKVYLPR284)-I-278. The introduced MDA modifications influenced profile of IgE reactivity to OVA. Treatment of T84 epithelial cells with OVA and OVA modified with 1 mM MDA, induced up-regulation of pro-inflammatory cytokines (IL-1 beta,IL-25, IL-33, TSLP and TNF alpha), while OVA modification with 10 mM MDA induced down regulation of the cytokine expression profile, except for IL-1 beta. OVA and OVA modified with 1 mM MDA induced secretion of epithelial cells specific cytokine IL-33. Conclusions: This finding indicated that OVA and its MDA modified form have the potential to trigger the innate immunity by inducing up-regulation and secretion of pro-allergenic IL-33 in T84 intestinal epithelial cells. General significance: Interactions of ovalbumin and its MDA modified form with intestinal epithelial cells can induce a specific immunological priming necessary for the downstream activation of innate immunity. (C) 2016 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Biochimica et Biophysica Acta: General Subjects
T1  - Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells
VL  - 1861
IS  - 2
SP  - 126
EP  - 134
DO  - 10.1016/j.bbagen.2016.11.021
ER  - 

@article{
author = "Nikolić, Jasna and Nešić, Andrijana N. and Čavić, Milena and Đorđević, Neda O. and Anđelković, Uroš and Atanasković-Marković, Marina and Drakulić, Branko J. and Gavrović-Jankulović, Marija",
year = "2017",
abstract = "ackground: Protein oxidation can occur as a consequence of lipid peroxidation during food processing. The aim of this work was to explore the effect of malondialdehyde (MDA) modification of ovalbumin (OVA) on its interaction with T84 intestinal cells. Methods: Molecular dynamics simulation was employed for the prediction of MDA modification in the OVA, while introduced structural changes were evaluated by measurement of carbonyl group content, fluorescence spectra, MS/MS analysis, and IgE reactivity. Effects of MDA modified OVA on T84 epithelial cells were analyzed by gene expression for pro-inflammatory cytokines and protein secretion. Results: Out of 9 predicted, five modified Lys residues were confirmed by MS/MS analysis: (51)TQINKVVR(58), (85)DILNQITKPNDVYSFSLASR(104), (111)YPILPEYLQCVKELYR(126), (187)AFKDEDTQAMPFR(99), (KIKVYLPR284)-K-277, and (IKVYLPR284)-I-278. The introduced MDA modifications influenced profile of IgE reactivity to OVA. Treatment of T84 epithelial cells with OVA and OVA modified with 1 mM MDA, induced up-regulation of pro-inflammatory cytokines (IL-1 beta,IL-25, IL-33, TSLP and TNF alpha), while OVA modification with 10 mM MDA induced down regulation of the cytokine expression profile, except for IL-1 beta. OVA and OVA modified with 1 mM MDA induced secretion of epithelial cells specific cytokine IL-33. Conclusions: This finding indicated that OVA and its MDA modified form have the potential to trigger the innate immunity by inducing up-regulation and secretion of pro-allergenic IL-33 in T84 intestinal epithelial cells. General significance: Interactions of ovalbumin and its MDA modified form with intestinal epithelial cells can induce a specific immunological priming necessary for the downstream activation of innate immunity. (C) 2016 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Biochimica et Biophysica Acta: General Subjects",
title = "Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells",
volume = "1861",
number = "2",
pages = "126-134",
doi = "10.1016/j.bbagen.2016.11.021"
}

Nikolić, J., Nešić, A. N., Čavić, M., Đorđević, N. O., Anđelković, U., Atanasković-Marković, M., Drakulić, B. J.,& Gavrović-Jankulović, M.. (2017). Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells. in Biochimica et Biophysica Acta: General Subjects
Elsevier Science Bv, Amsterdam., 1861(2), 126-134.
https://doi.org/10.1016/j.bbagen.2016.11.021

Nikolić J, Nešić AN, Čavić M, Đorđević NO, Anđelković U, Atanasković-Marković M, Drakulić BJ, Gavrović-Jankulović M. Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells. in Biochimica et Biophysica Acta: General Subjects. 2017;1861(2):126-134.
doi:10.1016/j.bbagen.2016.11.021 .

Nikolić, Jasna, Nešić, Andrijana N., Čavić, Milena, Đorđević, Neda O., Anđelković, Uroš, Atanasković-Marković, Marina, Drakulić, Branko J., Gavrović-Jankulović, Marija, "Effect of malondialdehyde on the ovalbumin structure and its interactions with T84 epithelial cells" in Biochimica et Biophysica Acta: General Subjects, 1861, no. 2 (2017):126-134,
https://doi.org/10.1016/j.bbagen.2016.11.021 . .

TY  - JOUR
AU  - Grozdanović, Milica M.
AU  - Čavić, Milena
AU  - Nešić, Andrijana N.
AU  - Anđelković, Uroš
AU  - Akbari, Peyman
AU  - Smit, Joost
AU  - Gavrović-Jankulović, Marija
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2039
AB  - Background: The intestinal epithelium forms a barrier that food allergens must cross in order to induce sensitization. The aim of this study was to evaluate the impact of the plant-derived food cysteine protease - actinidin (Act d1) on the integrity of intestinal epithelium tight junctions (TJs). Methods: Effects of Act d1 on the intestinal epithelium were evaluated in Caco-2 monolayers and in a mouse model by measuring transepithelial resistance and in vivo permeability. Integrity of the tight junctions was analyzed by confocal microscopy. Proteolysis of TJ protein occludin was evaluated by mass spectrometry. Results: Actinidin (1 mg/mL) reduced the transepithelial resistance of the cell monolayer by 18.1% (after 1 h) and 25.6% (after 4 h). This loss of barrier function was associated with Act d 1 disruption of the occludin and zonula occludens (ZO)-1 network. The effect on intestinal permeability in vivo was demonstrated by the significantly higher concentration of 40 kDa FITC-dextran (233 mu g/mL) that passed from the intestine into the serum of Act d1 treated mice in comparison to the control group (0.5 mu g/mL). Human occludin was fragmented, and putative Act d1 cleavage sites were identified in extracellular loops of human occludin. Conclusion: Act d1 caused protease-dependent disruption of tight junctions in confluent Caco-2 cells and increased intestinal permeability in mice. General significance: In line with the observed effects of food cysteine proteases in occupational allergy, these results suggest that disruption of tight junctions by food cysteine proteases may contribute to the process of sensitization in food allergy. (C) 2015 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Biochimica et Biophysica Acta: General Subjects
T1  - Kiwifruit cysteine protease actinidin compromises the intestinal barrier by disrupting tight junctions
VL  - 1860
IS  - 3
SP  - 516
EP  - 526
DO  - 10.1016/j.bbagen.2015.12.005
ER  - 

@article{
author = "Grozdanović, Milica M. and Čavić, Milena and Nešić, Andrijana N. and Anđelković, Uroš and Akbari, Peyman and Smit, Joost and Gavrović-Jankulović, Marija",
year = "2016",
abstract = "Background: The intestinal epithelium forms a barrier that food allergens must cross in order to induce sensitization. The aim of this study was to evaluate the impact of the plant-derived food cysteine protease - actinidin (Act d1) on the integrity of intestinal epithelium tight junctions (TJs). Methods: Effects of Act d1 on the intestinal epithelium were evaluated in Caco-2 monolayers and in a mouse model by measuring transepithelial resistance and in vivo permeability. Integrity of the tight junctions was analyzed by confocal microscopy. Proteolysis of TJ protein occludin was evaluated by mass spectrometry. Results: Actinidin (1 mg/mL) reduced the transepithelial resistance of the cell monolayer by 18.1% (after 1 h) and 25.6% (after 4 h). This loss of barrier function was associated with Act d 1 disruption of the occludin and zonula occludens (ZO)-1 network. The effect on intestinal permeability in vivo was demonstrated by the significantly higher concentration of 40 kDa FITC-dextran (233 mu g/mL) that passed from the intestine into the serum of Act d1 treated mice in comparison to the control group (0.5 mu g/mL). Human occludin was fragmented, and putative Act d1 cleavage sites were identified in extracellular loops of human occludin. Conclusion: Act d1 caused protease-dependent disruption of tight junctions in confluent Caco-2 cells and increased intestinal permeability in mice. General significance: In line with the observed effects of food cysteine proteases in occupational allergy, these results suggest that disruption of tight junctions by food cysteine proteases may contribute to the process of sensitization in food allergy. (C) 2015 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Biochimica et Biophysica Acta: General Subjects",
title = "Kiwifruit cysteine protease actinidin compromises the intestinal barrier by disrupting tight junctions",
volume = "1860",
number = "3",
pages = "516-526",
doi = "10.1016/j.bbagen.2015.12.005"
}

Grozdanović, M. M., Čavić, M., Nešić, A. N., Anđelković, U., Akbari, P., Smit, J.,& Gavrović-Jankulović, M.. (2016). Kiwifruit cysteine protease actinidin compromises the intestinal barrier by disrupting tight junctions. in Biochimica et Biophysica Acta: General Subjects
Elsevier Science Bv, Amsterdam., 1860(3), 516-526.
https://doi.org/10.1016/j.bbagen.2015.12.005

Grozdanović MM, Čavić M, Nešić AN, Anđelković U, Akbari P, Smit J, Gavrović-Jankulović M. Kiwifruit cysteine protease actinidin compromises the intestinal barrier by disrupting tight junctions. in Biochimica et Biophysica Acta: General Subjects. 2016;1860(3):516-526.
doi:10.1016/j.bbagen.2015.12.005 .

Grozdanović, Milica M., Čavić, Milena, Nešić, Andrijana N., Anđelković, Uroš, Akbari, Peyman, Smit, Joost, Gavrović-Jankulović, Marija, "Kiwifruit cysteine protease actinidin compromises the intestinal barrier by disrupting tight junctions" in Biochimica et Biophysica Acta: General Subjects, 1860, no. 3 (2016):516-526,
https://doi.org/10.1016/j.bbagen.2015.12.005 . .

TY  - JOUR
AU  - Grozdanović, Milica M.
AU  - Čavić, Milena
AU  - Nešić, Andrijana N.
AU  - Anđelković, Uroš
AU  - Akbari, Peyman
AU  - Smit, Joost
AU  - Gavrović-Jankulović, Marija
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3559
AB  - Background: The intestinal epithelium forms a barrier that food allergens must cross in order to induce sensitization. The aim of this study was to evaluate the impact of the plant-derived food cysteine protease - actinidin (Act d1) on the integrity of intestinal epithelium tight junctions (TJs). Methods: Effects of Act d1 on the intestinal epithelium were evaluated in Caco-2 monolayers and in a mouse model by measuring transepithelial resistance and in vivo permeability. Integrity of the tight junctions was analyzed by confocal microscopy. Proteolysis of TJ protein occludin was evaluated by mass spectrometry. Results: Actinidin (1 mg/mL) reduced the transepithelial resistance of the cell monolayer by 18.1% (after 1 h) and 25.6% (after 4 h). This loss of barrier function was associated with Act d 1 disruption of the occludin and zonula occludens (ZO)-1 network. The effect on intestinal permeability in vivo was demonstrated by the significantly higher concentration of 40 kDa FITC-dextran (233 mu g/mL) that passed from the intestine into the serum of Act d1 treated mice in comparison to the control group (0.5 mu g/mL). Human occludin was fragmented, and putative Act d1 cleavage sites were identified in extracellular loops of human occludin. Conclusion: Act d1 caused protease-dependent disruption of tight junctions in confluent Caco-2 cells and increased intestinal permeability in mice. General significance: In line with the observed effects of food cysteine proteases in occupational allergy, these results suggest that disruption of tight junctions by food cysteine proteases may contribute to the process of sensitization in food allergy. (C) 2015 Elsevier B.V. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Biochimica et Biophysica Acta: General Subjects
T1  - Kiwifruit cysteine protease actinidin compromises the intestinal barrier by disrupting tight junctions
VL  - 1860
IS  - 3
SP  - 516
EP  - 526
DO  - 10.1016/j.bbagen.2015.12.005
ER  - 

@article{
author = "Grozdanović, Milica M. and Čavić, Milena and Nešić, Andrijana N. and Anđelković, Uroš and Akbari, Peyman and Smit, Joost and Gavrović-Jankulović, Marija",
year = "2016",
abstract = "Background: The intestinal epithelium forms a barrier that food allergens must cross in order to induce sensitization. The aim of this study was to evaluate the impact of the plant-derived food cysteine protease - actinidin (Act d1) on the integrity of intestinal epithelium tight junctions (TJs). Methods: Effects of Act d1 on the intestinal epithelium were evaluated in Caco-2 monolayers and in a mouse model by measuring transepithelial resistance and in vivo permeability. Integrity of the tight junctions was analyzed by confocal microscopy. Proteolysis of TJ protein occludin was evaluated by mass spectrometry. Results: Actinidin (1 mg/mL) reduced the transepithelial resistance of the cell monolayer by 18.1% (after 1 h) and 25.6% (after 4 h). This loss of barrier function was associated with Act d 1 disruption of the occludin and zonula occludens (ZO)-1 network. The effect on intestinal permeability in vivo was demonstrated by the significantly higher concentration of 40 kDa FITC-dextran (233 mu g/mL) that passed from the intestine into the serum of Act d1 treated mice in comparison to the control group (0.5 mu g/mL). Human occludin was fragmented, and putative Act d1 cleavage sites were identified in extracellular loops of human occludin. Conclusion: Act d1 caused protease-dependent disruption of tight junctions in confluent Caco-2 cells and increased intestinal permeability in mice. General significance: In line with the observed effects of food cysteine proteases in occupational allergy, these results suggest that disruption of tight junctions by food cysteine proteases may contribute to the process of sensitization in food allergy. (C) 2015 Elsevier B.V. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Biochimica et Biophysica Acta: General Subjects",
title = "Kiwifruit cysteine protease actinidin compromises the intestinal barrier by disrupting tight junctions",
volume = "1860",
number = "3",
pages = "516-526",
doi = "10.1016/j.bbagen.2015.12.005"
}

Grozdanović, M. M., Čavić, M., Nešić, A. N., Anđelković, U., Akbari, P., Smit, J.,& Gavrović-Jankulović, M.. (2016). Kiwifruit cysteine protease actinidin compromises the intestinal barrier by disrupting tight junctions. in Biochimica et Biophysica Acta: General Subjects
Elsevier Science Bv, Amsterdam., 1860(3), 516-526.
https://doi.org/10.1016/j.bbagen.2015.12.005

Grozdanović MM, Čavić M, Nešić AN, Anđelković U, Akbari P, Smit J, Gavrović-Jankulović M. Kiwifruit cysteine protease actinidin compromises the intestinal barrier by disrupting tight junctions. in Biochimica et Biophysica Acta: General Subjects. 2016;1860(3):516-526.
doi:10.1016/j.bbagen.2015.12.005 .

Grozdanović, Milica M., Čavić, Milena, Nešić, Andrijana N., Anđelković, Uroš, Akbari, Peyman, Smit, Joost, Gavrović-Jankulović, Marija, "Kiwifruit cysteine protease actinidin compromises the intestinal barrier by disrupting tight junctions" in Biochimica et Biophysica Acta: General Subjects, 1860, no. 3 (2016):516-526,
https://doi.org/10.1016/j.bbagen.2015.12.005 . .