TY  - JOUR
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Gašić, Uroš M.
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Božunović, Jelena
AU  - Milutinović, Milica
AU  - Petrović, Luka
AU  - Dimitrijević, Milena
AU  - Anđelković, Boban D.
AU  - Mišić, Danijela
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6454
AB  - Iridoids, a class of atypical monoterpenes, exhibit exceptional diversity within the Nepeta genus (subfam. Nepetoidae, fam. Lamiaceae).The majority of these plants produce iridoids of the unique stereochemistry, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. By comparatively analyzing metabolomics, transcriptomics, gene co-expression, and phylogenetic data of the iridoid-producing N. rtanjensis Diklić & Milojević and iridoid-lacking N. nervosa Royle & Bentham, we presumed that one of the factors responsible for the absence of these compounds in N. nervosa is iridoid synthase (ISY). Two orthologues of ISY were mined from leaves transcriptome of N. rtanjensis (NrPRISE1 and NrPRISE2), while in N. nervosa only one (NnPRISE) was identified, and it was phylogenetically closer to the representatives of the Family 1 isoforms, designated as P5βRs. Organ-specific and MeJA-elicited profiling of iridoid content and co-expression analysis of IBG candidates, highlighted NrPRISE2 and NnPRISE as promising candidates for ISY orthologues, and their function was confirmed using in vitro assays with recombinant proteins, after heterologous expression of recombinant proteins in E. coli and their His-tag affinity purification. NrPRISE2 demonstrated ISY activity both in vitro and likely in planta, which was supported by the 3D modeling and molecular docking analysis, thus reclassification of NrPRISE2 to NrISY is accordingly recommended. NnPRISE also displays in vitro ISY-like activity, while its role under in vivo conditions was not here unambiguously confirmed. Most probably under in vivo conditions the NnPRISE lacks substrates to act upon, as a result of the loss of function of some of the upstream enzymes of the iridoid pathway. Our ongoing work is conducted towards re-establishing the biosynthesis of iridoids in N. nervosa.
PB  - Frontiers Media
T2  - Frontiers in Plant Science
T1  - Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae)
VL  - 14
DO  - 10.3389/fpls.2023.1211453
ER  - 

@article{
author = "Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Gašić, Uroš M. and Nestorović Živković, Jasmina and Dmitrović, Slavica and Božunović, Jelena and Milutinović, Milica and Petrović, Luka and Dimitrijević, Milena and Anđelković, Boban D. and Mišić, Danijela",
year = "2024",
abstract = "Iridoids, a class of atypical monoterpenes, exhibit exceptional diversity within the Nepeta genus (subfam. Nepetoidae, fam. Lamiaceae).The majority of these plants produce iridoids of the unique stereochemistry, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. By comparatively analyzing metabolomics, transcriptomics, gene co-expression, and phylogenetic data of the iridoid-producing N. rtanjensis Diklić & Milojević and iridoid-lacking N. nervosa Royle & Bentham, we presumed that one of the factors responsible for the absence of these compounds in N. nervosa is iridoid synthase (ISY). Two orthologues of ISY were mined from leaves transcriptome of N. rtanjensis (NrPRISE1 and NrPRISE2), while in N. nervosa only one (NnPRISE) was identified, and it was phylogenetically closer to the representatives of the Family 1 isoforms, designated as P5βRs. Organ-specific and MeJA-elicited profiling of iridoid content and co-expression analysis of IBG candidates, highlighted NrPRISE2 and NnPRISE as promising candidates for ISY orthologues, and their function was confirmed using in vitro assays with recombinant proteins, after heterologous expression of recombinant proteins in E. coli and their His-tag affinity purification. NrPRISE2 demonstrated ISY activity both in vitro and likely in planta, which was supported by the 3D modeling and molecular docking analysis, thus reclassification of NrPRISE2 to NrISY is accordingly recommended. NnPRISE also displays in vitro ISY-like activity, while its role under in vivo conditions was not here unambiguously confirmed. Most probably under in vivo conditions the NnPRISE lacks substrates to act upon, as a result of the loss of function of some of the upstream enzymes of the iridoid pathway. Our ongoing work is conducted towards re-establishing the biosynthesis of iridoids in N. nervosa.",
publisher = "Frontiers Media",
journal = "Frontiers in Plant Science",
title = "Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae)",
volume = "14",
doi = "10.3389/fpls.2023.1211453"
}

Aničić, N., Matekalo, D., Skorić, M., Gašić, U. M., Nestorović Živković, J., Dmitrović, S., Božunović, J., Milutinović, M., Petrović, L., Dimitrijević, M., Anđelković, B. D.,& Mišić, D.. (2024). Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae). in Frontiers in Plant Science
Frontiers Media., 14.
https://doi.org/10.3389/fpls.2023.1211453

Aničić N, Matekalo D, Skorić M, Gašić UM, Nestorović Živković J, Dmitrović S, Božunović J, Milutinović M, Petrović L, Dimitrijević M, Anđelković BD, Mišić D. Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae). in Frontiers in Plant Science. 2024;14.
doi:10.3389/fpls.2023.1211453 .

Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Gašić, Uroš M., Nestorović Živković, Jasmina, Dmitrović, Slavica, Božunović, Jelena, Milutinović, Milica, Petrović, Luka, Dimitrijević, Milena, Anđelković, Boban D., Mišić, Danijela, "Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae)" in Frontiers in Plant Science, 14 (2024),
https://doi.org/10.3389/fpls.2023.1211453 . .

TY  - JOUR
AU  - Milutinović, Milica
AU  - Nakarada, Đura
AU  - Božunović, Jelena
AU  - Todorović, Miloš
AU  - Gašić, Uroš M.
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Ivković, Đurđa
AU  - Savić, Jelena
AU  - Devrnja, Nina
AU  - Aničić, Neda
AU  - Banjanac, Tijana
AU  - Mojović, Miloš
AU  - Mišić, Danijela
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5896
AB  - The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.
PB  - MDPI
T2  - Antioxidants
T2  - Antioxidants
T1  - Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening
VL  - 12
IS  - 2
SP  - 346
DO  - 10.3390/antiox12020346
ER  - 

@article{
author = "Milutinović, Milica and Nakarada, Đura and Božunović, Jelena and Todorović, Miloš and Gašić, Uroš M. and Živković, Suzana and Skorić, Marijana and Ivković, Đurđa and Savić, Jelena and Devrnja, Nina and Aničić, Neda and Banjanac, Tijana and Mojović, Miloš and Mišić, Danijela",
year = "2023",
abstract = "The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.",
publisher = "MDPI",
journal = "Antioxidants, Antioxidants",
title = "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening",
volume = "12",
number = "2",
pages = "346",
doi = "10.3390/antiox12020346"
}

Milutinović, M., Nakarada, Đ., Božunović, J., Todorović, M., Gašić, U. M., Živković, S., Skorić, M., Ivković, Đ., Savić, J., Devrnja, N., Aničić, N., Banjanac, T., Mojović, M.,& Mišić, D.. (2023). Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants
MDPI., 12(2), 346.
https://doi.org/10.3390/antiox12020346

Milutinović M, Nakarada Đ, Božunović J, Todorović M, Gašić UM, Živković S, Skorić M, Ivković Đ, Savić J, Devrnja N, Aničić N, Banjanac T, Mojović M, Mišić D. Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants. 2023;12(2):346.
doi:10.3390/antiox12020346 .

Milutinović, Milica, Nakarada, Đura, Božunović, Jelena, Todorović, Miloš, Gašić, Uroš M., Živković, Suzana, Skorić, Marijana, Ivković, Đurđa, Savić, Jelena, Devrnja, Nina, Aničić, Neda, Banjanac, Tijana, Mojović, Miloš, Mišić, Danijela, "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening" in Antioxidants, 12, no. 2 (2023):346,
https://doi.org/10.3390/antiox12020346 . .

TY  - DATA
AU  - Božunović, Jelena
AU  - Živković, Suzana
AU  - Gašić, Uroš M.
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana D.
AU  - Matekalo, Dragana
AU  - Šiler, Branislav
AU  - Soković, Marina
AU  - Tešić, Živoslav Lj.
AU  - Mišić, Danijela
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3208
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - Supplementary material for the article: Božunović, J.; Živković, S.; Gašić, U.; Glamočlija, J.; Ćirić, A.; Matekalo, D.; Šiler, B.; Soković, M.; Tešić, Ž.; Mišić, D. In Vitro and in Vivo Transformations of Centaurium Erythraea Secoiridoid Glucosides Alternate Their Antioxidant and Antimicrobial Capacity. Industrial Crops and Products 2018, 111, 705–721. https://doi.org/10.1016/j.indcrop.2017.11.040
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3208
ER  - 

@misc{
author = "Božunović, Jelena and Živković, Suzana and Gašić, Uroš M. and Glamočlija, Jasmina and Ćirić, Ana D. and Matekalo, Dragana and Šiler, Branislav and Soković, Marina and Tešić, Živoslav Lj. and Mišić, Danijela",
year = "2018",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "Supplementary material for the article: Božunović, J.; Živković, S.; Gašić, U.; Glamočlija, J.; Ćirić, A.; Matekalo, D.; Šiler, B.; Soković, M.; Tešić, Ž.; Mišić, D. In Vitro and in Vivo Transformations of Centaurium Erythraea Secoiridoid Glucosides Alternate Their Antioxidant and Antimicrobial Capacity. Industrial Crops and Products 2018, 111, 705–721. https://doi.org/10.1016/j.indcrop.2017.11.040",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3208"
}

Božunović, J., Živković, S., Gašić, U. M., Glamočlija, J., Ćirić, A. D., Matekalo, D., Šiler, B., Soković, M., Tešić, Ž. Lj.,& Mišić, D.. (2018). Supplementary material for the article: Božunović, J.; Živković, S.; Gašić, U.; Glamočlija, J.; Ćirić, A.; Matekalo, D.; Šiler, B.; Soković, M.; Tešić, Ž.; Mišić, D. In Vitro and in Vivo Transformations of Centaurium Erythraea Secoiridoid Glucosides Alternate Their Antioxidant and Antimicrobial Capacity. Industrial Crops and Products 2018, 111, 705–721. https://doi.org/10.1016/j.indcrop.2017.11.040. in Industrial Crops and Products
Elsevier Science Bv, Amsterdam..
https://hdl.handle.net/21.15107/rcub_cherry_3208

Božunović J, Živković S, Gašić UM, Glamočlija J, Ćirić AD, Matekalo D, Šiler B, Soković M, Tešić ŽL, Mišić D. Supplementary material for the article: Božunović, J.; Živković, S.; Gašić, U.; Glamočlija, J.; Ćirić, A.; Matekalo, D.; Šiler, B.; Soković, M.; Tešić, Ž.; Mišić, D. In Vitro and in Vivo Transformations of Centaurium Erythraea Secoiridoid Glucosides Alternate Their Antioxidant and Antimicrobial Capacity. Industrial Crops and Products 2018, 111, 705–721. https://doi.org/10.1016/j.indcrop.2017.11.040. in Industrial Crops and Products. 2018;.
https://hdl.handle.net/21.15107/rcub_cherry_3208 .

Božunović, Jelena, Živković, Suzana, Gašić, Uroš M., Glamočlija, Jasmina, Ćirić, Ana D., Matekalo, Dragana, Šiler, Branislav, Soković, Marina, Tešić, Živoslav Lj., Mišić, Danijela, "Supplementary material for the article: Božunović, J.; Živković, S.; Gašić, U.; Glamočlija, J.; Ćirić, A.; Matekalo, D.; Šiler, B.; Soković, M.; Tešić, Ž.; Mišić, D. In Vitro and in Vivo Transformations of Centaurium Erythraea Secoiridoid Glucosides Alternate Their Antioxidant and Antimicrobial Capacity. Industrial Crops and Products 2018, 111, 705–721. https://doi.org/10.1016/j.indcrop.2017.11.040" in Industrial Crops and Products (2018),
https://hdl.handle.net/21.15107/rcub_cherry_3208 .