TY  - JOUR
AU  - Nikodinović-Runić, Jasmina
AU  - Martin, Leona B.
AU  - Babu, Ramesh P.
AU  - Blau, Werner
AU  - O'Connor, Kevin E.
PY  - 2009
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1008
AB  - Two melanin-overproducing Pseudomonas putida F6 mutants were generated using transposon (Tn5) mutagenesis. Mutants were disrupted in a transcriptional regulator (TR) and a homogentisate 1,2-dioxygenase (HDO) gene. Colonies of mutant F6-TR overproduced a black pigment on solid medium. The same mutant (F6-TR) had a 3.7-fold higher tyrosinase activity compared with the wild-type strain when induced with ferulic acid. However in tyrosine uptake assays whole cells of the mutant strain F6-TR consumed eight times less tyrosine compared with the wild-type strain. Mutant F6-HDO produced a diffusible red pigment into the growth medium. Pigment production by mutant F6-HDO is sixfold higher than the wild-type strain. The biomass yield of mutant F6-HDO grown on tyrosine as the sole source of carbon and energy was 1.2-fold lower than the wild-type strain. While the growth of the wild-type strain was completely inhibited by 5 min of exposure to UV light (254 nm) both mutant strains showed survival rates  gt  30%. Mutant F6-HDO was able to tolerate higher concentrations of hydrogen peroxide (H(2)O(2)) exhibiting 1.5 times smaller zones of inhibition at 10 mM H(2)O(2) compared with mutant F6-TR and the wild-type strain. The pigments produced by all strains were purified and confirmed to be melanins.
PB  - Wiley-Blackwell Publishing, Inc, Malden
T2  - FEMS Microbiology Letters / Federation of European Microbiological Societies
T1  - Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6
VL  - 298
IS  - 2
SP  - 174
EP  - 183
DO  - 10.1111/j.1574-6968.2009.01716.x
ER  - 

@article{
author = "Nikodinović-Runić, Jasmina and Martin, Leona B. and Babu, Ramesh P. and Blau, Werner and O'Connor, Kevin E.",
year = "2009",
abstract = "Two melanin-overproducing Pseudomonas putida F6 mutants were generated using transposon (Tn5) mutagenesis. Mutants were disrupted in a transcriptional regulator (TR) and a homogentisate 1,2-dioxygenase (HDO) gene. Colonies of mutant F6-TR overproduced a black pigment on solid medium. The same mutant (F6-TR) had a 3.7-fold higher tyrosinase activity compared with the wild-type strain when induced with ferulic acid. However in tyrosine uptake assays whole cells of the mutant strain F6-TR consumed eight times less tyrosine compared with the wild-type strain. Mutant F6-HDO produced a diffusible red pigment into the growth medium. Pigment production by mutant F6-HDO is sixfold higher than the wild-type strain. The biomass yield of mutant F6-HDO grown on tyrosine as the sole source of carbon and energy was 1.2-fold lower than the wild-type strain. While the growth of the wild-type strain was completely inhibited by 5 min of exposure to UV light (254 nm) both mutant strains showed survival rates  gt  30%. Mutant F6-HDO was able to tolerate higher concentrations of hydrogen peroxide (H(2)O(2)) exhibiting 1.5 times smaller zones of inhibition at 10 mM H(2)O(2) compared with mutant F6-TR and the wild-type strain. The pigments produced by all strains were purified and confirmed to be melanins.",
publisher = "Wiley-Blackwell Publishing, Inc, Malden",
journal = "FEMS Microbiology Letters / Federation of European Microbiological Societies",
title = "Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6",
volume = "298",
number = "2",
pages = "174-183",
doi = "10.1111/j.1574-6968.2009.01716.x"
}

Nikodinović-Runić, J., Martin, L. B., Babu, R. P., Blau, W.,& O'Connor, K. E.. (2009). Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6. in FEMS Microbiology Letters / Federation of European Microbiological Societies
Wiley-Blackwell Publishing, Inc, Malden., 298(2), 174-183.
https://doi.org/10.1111/j.1574-6968.2009.01716.x

Nikodinović-Runić J, Martin LB, Babu RP, Blau W, O'Connor KE. Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6. in FEMS Microbiology Letters / Federation of European Microbiological Societies. 2009;298(2):174-183.
doi:10.1111/j.1574-6968.2009.01716.x .

Nikodinović-Runić, Jasmina, Martin, Leona B., Babu, Ramesh P., Blau, Werner, O'Connor, Kevin E., "Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6" in FEMS Microbiology Letters / Federation of European Microbiological Societies, 298, no. 2 (2009):174-183,
https://doi.org/10.1111/j.1574-6968.2009.01716.x . .

TY  - JOUR
AU  - Nikodinović-Runić, Jasmina
AU  - Kenny, Shane T.
AU  - Babu, Ramesh P.
AU  - Woods, Trevor
AU  - Blau, Werner J.
AU  - O'Connor, Kevin E.
PY  - 2008
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/967
AB  - Here, we report the use of petrochemical aromatic hydrocarbons as a feedstock for the biotechnological conversion into valuable biodegradable plastic polymers-polyhydroxyalkanoates (PHAs). We assessed the ability of the known Pseudomonas putida species that are able to utilize benzene, toluene, ethylbenzene, p-xylene (BTEX) compounds as a sole carbon and energy source for their ability to produce PHA from the single substrates. P. putida F1 is able to accumulate medium-chain-length (mcl) PHA when supplied with toluene, benzene, or ethylbenzene. P. putida mt-2 accumulates mcl-PHA when supplied with toluene or p-xylene. The highest level of PHA accumulated by cultures in shake flask was 26% cell dry weight for P. putida mt-2 supplied with p-xylene. A synthetic mixture of benzene, toluene, ethylbenzene, p-xylene, and styrene (BTEXS) which mimics the aromatic fraction of mixed plastic pyrolysis oil was supplied to a defined mixed culture of P. putida F1, mt-2, and CA-3 in the shake flasks and fermentation experiments. PHA was accumulated to 24% and to 36% of the cell dry weight of the shake flask and fermentation grown cultures respectively. In addition a three-fold higher cell density was achieved with the mixed culture grown in the bioreactor compared to shake flask experiments. A run in the 5-l fermentor resulted in the utilization of 59.6 g (67.5 ml) of the BTEXS mixture and the production of 6 g of mcl-PHA. The monomer composition of PHA accumulated by the mixed culture was the same as that accumulated by single strains supplied with single substrates with 3-hydroxydecanoic acid occurring as the predominant monomer. The purified polymer was partially crystalline with an average molecular weight of 86.9 kDa. It has a thermal degradation temperature of 350 degrees C and a glass transition temperature of -48.5 degrees C.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate
VL  - 80
IS  - 4
SP  - 665
EP  - 673
DO  - 10.1007/s00253-008-1593-0
ER  - 

@article{
author = "Nikodinović-Runić, Jasmina and Kenny, Shane T. and Babu, Ramesh P. and Woods, Trevor and Blau, Werner J. and O'Connor, Kevin E.",
year = "2008",
abstract = "Here, we report the use of petrochemical aromatic hydrocarbons as a feedstock for the biotechnological conversion into valuable biodegradable plastic polymers-polyhydroxyalkanoates (PHAs). We assessed the ability of the known Pseudomonas putida species that are able to utilize benzene, toluene, ethylbenzene, p-xylene (BTEX) compounds as a sole carbon and energy source for their ability to produce PHA from the single substrates. P. putida F1 is able to accumulate medium-chain-length (mcl) PHA when supplied with toluene, benzene, or ethylbenzene. P. putida mt-2 accumulates mcl-PHA when supplied with toluene or p-xylene. The highest level of PHA accumulated by cultures in shake flask was 26% cell dry weight for P. putida mt-2 supplied with p-xylene. A synthetic mixture of benzene, toluene, ethylbenzene, p-xylene, and styrene (BTEXS) which mimics the aromatic fraction of mixed plastic pyrolysis oil was supplied to a defined mixed culture of P. putida F1, mt-2, and CA-3 in the shake flasks and fermentation experiments. PHA was accumulated to 24% and to 36% of the cell dry weight of the shake flask and fermentation grown cultures respectively. In addition a three-fold higher cell density was achieved with the mixed culture grown in the bioreactor compared to shake flask experiments. A run in the 5-l fermentor resulted in the utilization of 59.6 g (67.5 ml) of the BTEXS mixture and the production of 6 g of mcl-PHA. The monomer composition of PHA accumulated by the mixed culture was the same as that accumulated by single strains supplied with single substrates with 3-hydroxydecanoic acid occurring as the predominant monomer. The purified polymer was partially crystalline with an average molecular weight of 86.9 kDa. It has a thermal degradation temperature of 350 degrees C and a glass transition temperature of -48.5 degrees C.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate",
volume = "80",
number = "4",
pages = "665-673",
doi = "10.1007/s00253-008-1593-0"
}

Nikodinović-Runić, J., Kenny, S. T., Babu, R. P., Woods, T., Blau, W. J.,& O'Connor, K. E.. (2008). The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate. in Applied Microbiology and Biotechnology
Springer, New York., 80(4), 665-673.
https://doi.org/10.1007/s00253-008-1593-0

Nikodinović-Runić J, Kenny ST, Babu RP, Woods T, Blau WJ, O'Connor KE. The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate. in Applied Microbiology and Biotechnology. 2008;80(4):665-673.
doi:10.1007/s00253-008-1593-0 .

Nikodinović-Runić, Jasmina, Kenny, Shane T., Babu, Ramesh P., Woods, Trevor, Blau, Werner J., O'Connor, Kevin E., "The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate" in Applied Microbiology and Biotechnology, 80, no. 4 (2008):665-673,
https://doi.org/10.1007/s00253-008-1593-0 . .

TY  - JOUR
AU  - Kenny, Shane T.
AU  - Nikodinović-Runić, Jasmina
AU  - Kaminsky, Walter
AU  - Woods, Trevor
AU  - Babu, Ramesh P.
AU  - Keely, Chris M.
AU  - Blau, Werner
AU  - O'Connor, Kevin E.
PY  - 2008
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/978
AB  - The conversion of the petrochemical polymer polyethylene terephthalate (PET) to a biodegradable plastic polyhydroxyalkanoate (PHA) is described here. PET was pyrolised at 450 degrees C resulting in the production of a solid, liquid, and gaseous fraction. The liquid and gaseous fractions were burnt for energy recovery, whereas the solid fraction terephthalic acid (TA) was used as the feedstock for bacterial production of PHA. Strains previously reported to grow on TA were unable to accumulate PHA. We therefore isolated bacteria from soil exposed to PET granules at a PET bottle processing plant. From the 32 strains isolated, three strains capable of accumulation of medium chain length PHA (mclPHA) from TA as a sole source of carbon and energy were selected for further study. These isolates were identified using 16S rDNA techniques as P. putida (GO16), P. putida (GO19), and P. frederiksbergensis (GO23). P. putida GO16 and GO19 accumulate PHA composed predominantly of a 3-hydroxydecanoic acid monomer while P. frederiksbergensis GO23 accumulates 3-hydroxydecanoic acid as the predominant monomer with increased amounts of 3-hydroxydodecanoic acid and 3-hydroxydodecenoic acid compared to the other two strains. PHA was detected in all three strains when nitrogen depleted below detectable levels in the growth medium. Strains GO16 and GO19 accumulate PHA at a maximal rate of approximately 8.4 mg PHA/l/h for 12 h before the rate of PHA accumulation decreased dramatically. Strain GO23 accumulates PHA at a lower maximal rate of 4.4 mg PHA/l/h but there was no slow down in the rate of PHA accumulation over time. Each of the PHA polymers is a thermoplastic with the onset of thermal degradation occurring around 308 degrees C with the complete degradation occurring by 370 degrees C. The molecular weight ranged from 74 to 123 kDa. X-ray diffraction indicated crystallinity of the order of 18-31%. Thermal analysis shows a low glass transition (-53 degrees C) with a broad melting endotherm between 0 and 45 degrees C.
PB  - Amer Chemical Soc, Washington
T2  - Environmental Science and Technology
T1  - Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)
VL  - 42
IS  - 20
SP  - 7696
EP  - 7701
DO  - 10.1021/es801010e
ER  - 

@article{
author = "Kenny, Shane T. and Nikodinović-Runić, Jasmina and Kaminsky, Walter and Woods, Trevor and Babu, Ramesh P. and Keely, Chris M. and Blau, Werner and O'Connor, Kevin E.",
year = "2008",
abstract = "The conversion of the petrochemical polymer polyethylene terephthalate (PET) to a biodegradable plastic polyhydroxyalkanoate (PHA) is described here. PET was pyrolised at 450 degrees C resulting in the production of a solid, liquid, and gaseous fraction. The liquid and gaseous fractions were burnt for energy recovery, whereas the solid fraction terephthalic acid (TA) was used as the feedstock for bacterial production of PHA. Strains previously reported to grow on TA were unable to accumulate PHA. We therefore isolated bacteria from soil exposed to PET granules at a PET bottle processing plant. From the 32 strains isolated, three strains capable of accumulation of medium chain length PHA (mclPHA) from TA as a sole source of carbon and energy were selected for further study. These isolates were identified using 16S rDNA techniques as P. putida (GO16), P. putida (GO19), and P. frederiksbergensis (GO23). P. putida GO16 and GO19 accumulate PHA composed predominantly of a 3-hydroxydecanoic acid monomer while P. frederiksbergensis GO23 accumulates 3-hydroxydecanoic acid as the predominant monomer with increased amounts of 3-hydroxydodecanoic acid and 3-hydroxydodecenoic acid compared to the other two strains. PHA was detected in all three strains when nitrogen depleted below detectable levels in the growth medium. Strains GO16 and GO19 accumulate PHA at a maximal rate of approximately 8.4 mg PHA/l/h for 12 h before the rate of PHA accumulation decreased dramatically. Strain GO23 accumulates PHA at a lower maximal rate of 4.4 mg PHA/l/h but there was no slow down in the rate of PHA accumulation over time. Each of the PHA polymers is a thermoplastic with the onset of thermal degradation occurring around 308 degrees C with the complete degradation occurring by 370 degrees C. The molecular weight ranged from 74 to 123 kDa. X-ray diffraction indicated crystallinity of the order of 18-31%. Thermal analysis shows a low glass transition (-53 degrees C) with a broad melting endotherm between 0 and 45 degrees C.",
publisher = "Amer Chemical Soc, Washington",
journal = "Environmental Science and Technology",
title = "Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)",
volume = "42",
number = "20",
pages = "7696-7701",
doi = "10.1021/es801010e"
}

Kenny, S. T., Nikodinović-Runić, J., Kaminsky, W., Woods, T., Babu, R. P., Keely, C. M., Blau, W.,& O'Connor, K. E.. (2008). Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate). in Environmental Science and Technology
Amer Chemical Soc, Washington., 42(20), 7696-7701.
https://doi.org/10.1021/es801010e

Kenny ST, Nikodinović-Runić J, Kaminsky W, Woods T, Babu RP, Keely CM, Blau W, O'Connor KE. Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate). in Environmental Science and Technology. 2008;42(20):7696-7701.
doi:10.1021/es801010e .

Kenny, Shane T., Nikodinović-Runić, Jasmina, Kaminsky, Walter, Woods, Trevor, Babu, Ramesh P., Keely, Chris M., Blau, Werner, O'Connor, Kevin E., "Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)" in Environmental Science and Technology, 42, no. 20 (2008):7696-7701,
https://doi.org/10.1021/es801010e . .