TY  - JOUR
AU  - Jovanović, Vesna B.
AU  - Aćimović, Jelena M.
AU  - Sreckovic, Vesna S. Dimitrijevic
AU  - Mandić, Ljuba M.
PY  - 2014
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1647
AB  - It was verified that the serum N-acetyl-beta-D-glucosaminidase (NAG) activity is elevated in diabetes, but there are no reports about changes in the sialic acid (SA) content in the carbohydrate parts of the NAG A form and its influence on the total changes in NAG activity in type 1 diabetes mellitus patients with and without secondary complications. The NAG A form was isolated from the serum of 81 insulin-dependent diabetes mellitus (IDDM) patients with and without secondary complications (retinopathy, polyneuropathy and nephropathy) and 25 healthy persons, and purified and characterised. The content of alpha-2,6-bound SA, the isoenzyme patterns of the purified A form, and the total NAG and A form activities were determined. In all diabetic groups, the sialylation levels of the A form were 2-3.5 times lower compared to control, while their acidities (fractions with pI 4.25-5.1) increased, particularly with progression of secondary complications. Total serum NAG activities and percentages of A form were significantly higher (P  lt  0.001) in all diabetic groups and negatively correlated with the alpha-2,6-bound SA content of the A form. In addition, they decreased as secondary diabetic complications became more complex. The observed changes could be the consequence of structural changes in the A form due to significant increases in its acidity, i.e., negative charge, which originated from groups other than SA.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity?
VL  - 79
IS  - 12
SP  - 1491
EP  - 1503
DO  - 10.2298/JSC140430076J
ER  - 

@article{
author = "Jovanović, Vesna B. and Aćimović, Jelena M. and Sreckovic, Vesna S. Dimitrijevic and Mandić, Ljuba M.",
year = "2014",
abstract = "It was verified that the serum N-acetyl-beta-D-glucosaminidase (NAG) activity is elevated in diabetes, but there are no reports about changes in the sialic acid (SA) content in the carbohydrate parts of the NAG A form and its influence on the total changes in NAG activity in type 1 diabetes mellitus patients with and without secondary complications. The NAG A form was isolated from the serum of 81 insulin-dependent diabetes mellitus (IDDM) patients with and without secondary complications (retinopathy, polyneuropathy and nephropathy) and 25 healthy persons, and purified and characterised. The content of alpha-2,6-bound SA, the isoenzyme patterns of the purified A form, and the total NAG and A form activities were determined. In all diabetic groups, the sialylation levels of the A form were 2-3.5 times lower compared to control, while their acidities (fractions with pI 4.25-5.1) increased, particularly with progression of secondary complications. Total serum NAG activities and percentages of A form were significantly higher (P  lt  0.001) in all diabetic groups and negatively correlated with the alpha-2,6-bound SA content of the A form. In addition, they decreased as secondary diabetic complications became more complex. The observed changes could be the consequence of structural changes in the A form due to significant increases in its acidity, i.e., negative charge, which originated from groups other than SA.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity?",
volume = "79",
number = "12",
pages = "1491-1503",
doi = "10.2298/JSC140430076J"
}

Jovanović, V. B., Aćimović, J. M., Sreckovic, V. S. D.,& Mandić, L. M.. (2014). How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity?. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 79(12), 1491-1503.
https://doi.org/10.2298/JSC140430076J

Jovanović VB, Aćimović JM, Sreckovic VSD, Mandić LM. How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity?. in Journal of the Serbian Chemical Society. 2014;79(12):1491-1503.
doi:10.2298/JSC140430076J .

Jovanović, Vesna B., Aćimović, Jelena M., Sreckovic, Vesna S. Dimitrijevic, Mandić, Ljuba M., "How the sialylation level of serum N-acetyl-beta-D-glucosaminidase A form in Type 1 diabetes mellitus influences their activity?" in Journal of the Serbian Chemical Society, 79, no. 12 (2014):1491-1503,
https://doi.org/10.2298/JSC140430076J . .