TY  - JOUR
AU  - Topakas, Evangelos
AU  - Siaperas, Romanos
AU  - Taxeidis, George
AU  - Makryniotis, Konstantinos
AU  - Guzik, Maciej
AU  - Maslak, Veselin
AU  - Beškoski, Vladimir
AU  - Lončarević, Branka
AU  - Ilić-Tomić, Tatjana
AU  - Milivojević, Dušan
AU  - Škaro Bogojević, Sanja
AU  - Pantelić, Brana
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5969
AB  - Polyurethanes (PUs) are an exceedingly heterogeneous group of plastic polymers, widely used in a variety of industries from construction to medical implants. In the past decades, we have witnessed the accumulation of PU waste and its detrimental environmental impacts. PUs have been identified as one of the most toxic polymers leaching hazardous compounds derived both from the polymer itself and the additives used in production. Further environmental impact assessment, identification and characterization of substances derived from PU materials and establishing efficient degradation strategies are crucial. Thus, a selection of eight synthetic model compounds which represent partial PU hydrolysis products were synthesized and characterized both in terms of toxicity and suitability to be used as substrates for the identification of novel biocatalysts for PU biodegradation. Overall, the compounds exhibited low in vitro cytotoxicity against a healthy human fibroblast cell line and virtually no toxic effect on the nematode Caenorhabditis elegans up to 500 µg mL−1, and two of the substrates showed moderate aquatic ecotoxicity with EC50 values 53 µg mL−1 and 45 µg mL−1, respectively, on Aliivibrio fischeri. The compounds were successfully applied to study the mechanism of ester and urethane bond cleaving preference of known plastic-degrading enzymes and were used to single out a novel PU-degrading biocatalyst, Amycolatopsis mediterranei ISP5501, among 220 microbial strains. A. mediterranei ISP5501 can also degrade commercially available polyether and polyester PU materials, reducing the average molecular number of the polymer up to 13.5%. This study uncovered a biocatalyst capable of degrading different types of PUs and identified potential enzymes responsible as a key step in developing biotechnological process for PU waste treatment options.
PB  - MDPI
T2  - Catalysts
T1  - Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts
VL  - 13
IS  - 2
SP  - 278
DO  - 10.3390/catal13020278
ER  - 

@article{
author = "Topakas, Evangelos and Siaperas, Romanos and Taxeidis, George and Makryniotis, Konstantinos and Guzik, Maciej and Maslak, Veselin and Beškoski, Vladimir and Lončarević, Branka and Ilić-Tomić, Tatjana and Milivojević, Dušan and Škaro Bogojević, Sanja and Pantelić, Brana",
year = "2023",
abstract = "Polyurethanes (PUs) are an exceedingly heterogeneous group of plastic polymers, widely used in a variety of industries from construction to medical implants. In the past decades, we have witnessed the accumulation of PU waste and its detrimental environmental impacts. PUs have been identified as one of the most toxic polymers leaching hazardous compounds derived both from the polymer itself and the additives used in production. Further environmental impact assessment, identification and characterization of substances derived from PU materials and establishing efficient degradation strategies are crucial. Thus, a selection of eight synthetic model compounds which represent partial PU hydrolysis products were synthesized and characterized both in terms of toxicity and suitability to be used as substrates for the identification of novel biocatalysts for PU biodegradation. Overall, the compounds exhibited low in vitro cytotoxicity against a healthy human fibroblast cell line and virtually no toxic effect on the nematode Caenorhabditis elegans up to 500 µg mL−1, and two of the substrates showed moderate aquatic ecotoxicity with EC50 values 53 µg mL−1 and 45 µg mL−1, respectively, on Aliivibrio fischeri. The compounds were successfully applied to study the mechanism of ester and urethane bond cleaving preference of known plastic-degrading enzymes and were used to single out a novel PU-degrading biocatalyst, Amycolatopsis mediterranei ISP5501, among 220 microbial strains. A. mediterranei ISP5501 can also degrade commercially available polyether and polyester PU materials, reducing the average molecular number of the polymer up to 13.5%. This study uncovered a biocatalyst capable of degrading different types of PUs and identified potential enzymes responsible as a key step in developing biotechnological process for PU waste treatment options.",
publisher = "MDPI",
journal = "Catalysts",
title = "Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts",
volume = "13",
number = "2",
pages = "278",
doi = "10.3390/catal13020278"
}

Topakas, E., Siaperas, R., Taxeidis, G., Makryniotis, K., Guzik, M., Maslak, V., Beškoski, V., Lončarević, B., Ilić-Tomić, T., Milivojević, D., Škaro Bogojević, S.,& Pantelić, B.. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. in Catalysts
MDPI., 13(2), 278.
https://doi.org/10.3390/catal13020278

Topakas E, Siaperas R, Taxeidis G, Makryniotis K, Guzik M, Maslak V, Beškoski V, Lončarević B, Ilić-Tomić T, Milivojević D, Škaro Bogojević S, Pantelić B. Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. in Catalysts. 2023;13(2):278.
doi:10.3390/catal13020278 .

Topakas, Evangelos, Siaperas, Romanos, Taxeidis, George, Makryniotis, Konstantinos, Guzik, Maciej, Maslak, Veselin, Beškoski, Vladimir, Lončarević, Branka, Ilić-Tomić, Tatjana, Milivojević, Dušan, Škaro Bogojević, Sanja, Pantelić, Brana, "Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts" in Catalysts, 13, no. 2 (2023):278,
https://doi.org/10.3390/catal13020278 . .

TY  - GEN
AU  - Beškoski, Vladimir
AU  - Lješević, Marija
AU  - Lončarević, Branka
AU  - Božić, Tatjana T.
AU  - Relić, Dubravka
AU  - Vujisić, Ljubodrag V.
AU  - Gruden-Pavlović, Maja
AU  - Lugonja, Nikoleta
AU  - Jiménez, Begoña
AU  - Colomer Vidal, Pere
AU  - Muñoz Arnanz, Juan
AU  - Battaglia, Fabienne
AU  - Crampon, Marc
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6048
AB  - Per- and polyfluoroalkyl substances (PFAS) are a group of synthetic chemicals that have been widely used in various industrial and consumer products due to their unique properties, such as oil and water repellency, thermal stability, and durability. However, the persistence and mobility of these chemicals in the environment have raised concerns about their potential adverse effects on human health and the environment. PFAS have been detected on a global scale in various environmental media, such as soil, water, air, and biota. As a country undergoing economic development and transition, Serbia faces the challenge of managing and addressing the PFAS contamination in its environment. This challenge requires a comprehensive and science-based strategy that can effectively reduce the exposure and risks of PFAS to human health and the environment. This document aims to provide a scientific strategy for solving the PFAS challenge in Serbia. Firstly, it is important to acknowledge that PFAS are persistent and bioaccumulative in the environment, which means that they do not break down easily and can accumulate in the food chain, posing a long-term risk to human health and the environment. Therefore, a precautionary approach is necessary to minimize the exposure and risks of PFAS. International experience and cooperation are very important for developing an effective scientifc strategy for addressing the PFAS challenge in Serbia. PFAS are a global issue, and many countries have already implemented measures to manage and reduce the exposure and risks of PFAS. Therefore, it is important to draw on international experience and best practices when developing the strategy for Serbia. International experience can provide valuable insights into the sources, pathways, and fate of PFAS, as well as the e"ectiveness of various risk management measures. For example, the United States, Canada, and some European countries have established regulatory frameworks for PFAS, which can serve as a model for Serbia. Other countries have implemented remediation measures for contaminated sites, which can provide valuable insights for selecting appropriate remediation technologies in Serbia. Moreover, international experience can provide access to the latest scientific knowledge, methods, and technologies for assessing and managing PFAS contamination. For example, international organizations such as the United Nations Environment Programme (UNEP) and the Organization for Economic Co-operation and Development (OECD) have developed guidance documents and tools for assessing and managing PFAS contamination. The scientific strategy for solving the PFAS challenge in Serbia is based on a thorough understanding of the sources, pathways, and fate of PFAS in the environment. It is also based on a comprehensive and systematic approach, including risk assessment, monitoring, regulation, remediation, and communication. This strategy is tailored to the specific context and needs of Serbia. It is based on the latest scientifc knowledge and practical experience from other countries and regions, focusing on the European Union, USA, China and Japan. The cultural, social, economic, and political factors can affect the implementation and effectiveness of the strategy, and therefore, the strategy is developed through a collaborative and participatory process involving stakeholders from different sectors and levels. The international experience provided helpful guidance and lessons learned, but ultimately, the strategy is based on local knowledge, priorities, and capacities. By implementing this strategy, we aim to contribute to that Serbia can protect its citizens and environment from the potential harm of PFAS and achieve sustainable development.
PB  - University of Belgrade – Faculty of Chemistry
T1  - Scientifc Strategy for PFAS Analysis and Bioremediation at UBFC  (2023-2033)
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6048
ER  - 

@misc{
author = "Beškoski, Vladimir and Lješević, Marija and Lončarević, Branka and Božić, Tatjana T. and Relić, Dubravka and Vujisić, Ljubodrag V. and Gruden-Pavlović, Maja and Lugonja, Nikoleta and Jiménez, Begoña and Colomer Vidal, Pere and Muñoz Arnanz, Juan and Battaglia, Fabienne and Crampon, Marc",
year = "2023",
abstract = "Per- and polyfluoroalkyl substances (PFAS) are a group of synthetic chemicals that have been widely used in various industrial and consumer products due to their unique properties, such as oil and water repellency, thermal stability, and durability. However, the persistence and mobility of these chemicals in the environment have raised concerns about their potential adverse effects on human health and the environment. PFAS have been detected on a global scale in various environmental media, such as soil, water, air, and biota. As a country undergoing economic development and transition, Serbia faces the challenge of managing and addressing the PFAS contamination in its environment. This challenge requires a comprehensive and science-based strategy that can effectively reduce the exposure and risks of PFAS to human health and the environment. This document aims to provide a scientific strategy for solving the PFAS challenge in Serbia. Firstly, it is important to acknowledge that PFAS are persistent and bioaccumulative in the environment, which means that they do not break down easily and can accumulate in the food chain, posing a long-term risk to human health and the environment. Therefore, a precautionary approach is necessary to minimize the exposure and risks of PFAS. International experience and cooperation are very important for developing an effective scientifc strategy for addressing the PFAS challenge in Serbia. PFAS are a global issue, and many countries have already implemented measures to manage and reduce the exposure and risks of PFAS. Therefore, it is important to draw on international experience and best practices when developing the strategy for Serbia. International experience can provide valuable insights into the sources, pathways, and fate of PFAS, as well as the e"ectiveness of various risk management measures. For example, the United States, Canada, and some European countries have established regulatory frameworks for PFAS, which can serve as a model for Serbia. Other countries have implemented remediation measures for contaminated sites, which can provide valuable insights for selecting appropriate remediation technologies in Serbia. Moreover, international experience can provide access to the latest scientific knowledge, methods, and technologies for assessing and managing PFAS contamination. For example, international organizations such as the United Nations Environment Programme (UNEP) and the Organization for Economic Co-operation and Development (OECD) have developed guidance documents and tools for assessing and managing PFAS contamination. The scientific strategy for solving the PFAS challenge in Serbia is based on a thorough understanding of the sources, pathways, and fate of PFAS in the environment. It is also based on a comprehensive and systematic approach, including risk assessment, monitoring, regulation, remediation, and communication. This strategy is tailored to the specific context and needs of Serbia. It is based on the latest scientifc knowledge and practical experience from other countries and regions, focusing on the European Union, USA, China and Japan. The cultural, social, economic, and political factors can affect the implementation and effectiveness of the strategy, and therefore, the strategy is developed through a collaborative and participatory process involving stakeholders from different sectors and levels. The international experience provided helpful guidance and lessons learned, but ultimately, the strategy is based on local knowledge, priorities, and capacities. By implementing this strategy, we aim to contribute to that Serbia can protect its citizens and environment from the potential harm of PFAS and achieve sustainable development.",
publisher = "University of Belgrade – Faculty of Chemistry",
title = "Scientifc Strategy for PFAS Analysis and Bioremediation at UBFC  (2023-2033)",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6048"
}

Beškoski, V., Lješević, M., Lončarević, B., Božić, T. T., Relić, D., Vujisić, L. V., Gruden-Pavlović, M., Lugonja, N., Jiménez, B., Colomer Vidal, P., Muñoz Arnanz, J., Battaglia, F.,& Crampon, M.. (2023). Scientifc Strategy for PFAS Analysis and Bioremediation at UBFC  (2023-2033). 
University of Belgrade – Faculty of Chemistry..
https://hdl.handle.net/21.15107/rcub_cherry_6048

Beškoski V, Lješević M, Lončarević B, Božić TT, Relić D, Vujisić LV, Gruden-Pavlović M, Lugonja N, Jiménez B, Colomer Vidal P, Muñoz Arnanz J, Battaglia F, Crampon M. Scientifc Strategy for PFAS Analysis and Bioremediation at UBFC  (2023-2033). 2023;.
https://hdl.handle.net/21.15107/rcub_cherry_6048 .

Beškoski, Vladimir, Lješević, Marija, Lončarević, Branka, Božić, Tatjana T., Relić, Dubravka, Vujisić, Ljubodrag V., Gruden-Pavlović, Maja, Lugonja, Nikoleta, Jiménez, Begoña, Colomer Vidal, Pere, Muñoz Arnanz, Juan, Battaglia, Fabienne, Crampon, Marc, "Scientifc Strategy for PFAS Analysis and Bioremediation at UBFC  (2023-2033)" (2023),
https://hdl.handle.net/21.15107/rcub_cherry_6048 .

TY  - CONF
AU  - Bubanja, Itana Nuša
AU  - Lončarević, Branka D.
AU  - Lješević, Marija
AU  - Stanisavljev, Dragomir
AU  - Beškoski, Vladimir
AU  - Gojgić-Cvijović, Gordana D.
AU  - Bajuk-Bogdanović, Danica
AU  - Gizdavić-Nikolaidis, Marija
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5430
AB  - Microwave assisted synthesis of polyaniline modified by pullulan (PANI/Pull) composite wasperformed by aniline oxidation with potassium iodate. The PANI/Pull composite was characterizedusing ATR-FTIR technique. FTIR spectra confirm presence of both components in PANI/Pullcomposite. Antimicrobial evaluation of PANI/Pull material performed by using a qualitative diskdiffusion method on Candida albicans (C. albicans) culture showed very high sensitivity toPANI/Pull composite. Observed FTIR and antifungal activity represent a promising results especiallyfor potential biomedical applications of PANI/Pull composite.
PB  - Society of Physical Chemists of Serbia
C3  - Proceedings - Physical Chemistry 2021, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Microwave assisted synthesis of polyaniline/pullulan (pani/pull) composite
VL  - 2
SP  - 445
EP  - 448
UR  - https://hdl.handle.net/21.15107/rcub_cer_4955
ER  - 

@conference{
author = "Bubanja, Itana Nuša and Lončarević, Branka D. and Lješević, Marija and Stanisavljev, Dragomir and Beškoski, Vladimir and Gojgić-Cvijović, Gordana D. and Bajuk-Bogdanović, Danica and Gizdavić-Nikolaidis, Marija",
year = "2021",
abstract = "Microwave assisted synthesis of polyaniline modified by pullulan (PANI/Pull) composite wasperformed by aniline oxidation with potassium iodate. The PANI/Pull composite was characterizedusing ATR-FTIR technique. FTIR spectra confirm presence of both components in PANI/Pullcomposite. Antimicrobial evaluation of PANI/Pull material performed by using a qualitative diskdiffusion method on Candida albicans (C. albicans) culture showed very high sensitivity toPANI/Pull composite. Observed FTIR and antifungal activity represent a promising results especiallyfor potential biomedical applications of PANI/Pull composite.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Proceedings - Physical Chemistry 2021, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Microwave assisted synthesis of polyaniline/pullulan (pani/pull) composite",
volume = "2",
pages = "445-448",
url = "https://hdl.handle.net/21.15107/rcub_cer_4955"
}

Bubanja, I. N., Lončarević, B. D., Lješević, M., Stanisavljev, D., Beškoski, V., Gojgić-Cvijović, G. D., Bajuk-Bogdanović, D.,& Gizdavić-Nikolaidis, M.. (2021). Microwave assisted synthesis of polyaniline/pullulan (pani/pull) composite. in Proceedings - Physical Chemistry 2021, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., 2, 445-448.
https://hdl.handle.net/21.15107/rcub_cer_4955

Bubanja IN, Lončarević BD, Lješević M, Stanisavljev D, Beškoski V, Gojgić-Cvijović GD, Bajuk-Bogdanović D, Gizdavić-Nikolaidis M. Microwave assisted synthesis of polyaniline/pullulan (pani/pull) composite. in Proceedings - Physical Chemistry 2021, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2021;2:445-448.
https://hdl.handle.net/21.15107/rcub_cer_4955 .

Bubanja, Itana Nuša, Lončarević, Branka D., Lješević, Marija, Stanisavljev, Dragomir, Beškoski, Vladimir, Gojgić-Cvijović, Gordana D., Bajuk-Bogdanović, Danica, Gizdavić-Nikolaidis, Marija, "Microwave assisted synthesis of polyaniline/pullulan (pani/pull) composite" in Proceedings - Physical Chemistry 2021, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 2 (2021):445-448,
https://hdl.handle.net/21.15107/rcub_cer_4955 .

TY  - CONF
AU  - Bubanja, Itana Nuša
AU  - Lončarević, Branka D.
AU  - Lješević, Marija
AU  - Stanisavljev, Dragomir
AU  - Beškoski, Vladimir
AU  - Gojgić-Cvijović, Gordana D.
AU  - Bajuk-Bogdanović, Danica
AU  - Gizdavić-Nikolaidis, Marija
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5431
AB  - Poster presented at Physical Chemistry 2021, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Microwave assisted synthesis of polyaniline/pullulan (pani/pull) composite
UR  - https://hdl.handle.net/21.15107/rcub_cer_4956
ER  - 

@conference{
author = "Bubanja, Itana Nuša and Lončarević, Branka D. and Lješević, Marija and Stanisavljev, Dragomir and Beškoski, Vladimir and Gojgić-Cvijović, Gordana D. and Bajuk-Bogdanović, Danica and Gizdavić-Nikolaidis, Marija",
year = "2021",
abstract = "Poster presented at Physical Chemistry 2021, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Microwave assisted synthesis of polyaniline/pullulan (pani/pull) composite",
url = "https://hdl.handle.net/21.15107/rcub_cer_4956"
}

Bubanja, I. N., Lončarević, B. D., Lješević, M., Stanisavljev, D., Beškoski, V., Gojgić-Cvijović, G. D., Bajuk-Bogdanović, D.,& Gizdavić-Nikolaidis, M.. (2021). Microwave assisted synthesis of polyaniline/pullulan (pani/pull) composite. .
https://hdl.handle.net/21.15107/rcub_cer_4956

Bubanja IN, Lončarević BD, Lješević M, Stanisavljev D, Beškoski V, Gojgić-Cvijović GD, Bajuk-Bogdanović D, Gizdavić-Nikolaidis M. Microwave assisted synthesis of polyaniline/pullulan (pani/pull) composite. 2021;.
https://hdl.handle.net/21.15107/rcub_cer_4956 .

Bubanja, Itana Nuša, Lončarević, Branka D., Lješević, Marija, Stanisavljev, Dragomir, Beškoski, Vladimir, Gojgić-Cvijović, Gordana D., Bajuk-Bogdanović, Danica, Gizdavić-Nikolaidis, Marija, "Microwave assisted synthesis of polyaniline/pullulan (pani/pull) composite" (2021),
https://hdl.handle.net/21.15107/rcub_cer_4956 .

TY  - JOUR
AU  - Lugonja, Nikoleta
AU  - Lončarević, Branka D.
AU  - Stanković, Dalibor
AU  - Marinković, Vesna S.
AU  - Lješević, Marija
AU  - Spasić, Snežana
AU  - Beškoski, Vladimir
PY  - 2021
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4432
AB  - BACKGROUND: Pectin is a complex polysaccharide, present in fresh and processed food as an additive, which also serves as an important source of fiber. In this work, the aim was to investigate in-vitro application of apple pectin as a possible antimicrobial, antioxidant and prebiotic agent for growth of bacteria selected from human milk and infant feces, as well as standard strains Lactobacillus rhamnosus and Bifidobacterium bifidum.
METHODS : All the bacteria isolated from the milk of mothers of preterm and term-born infants, bacteria from infant feces and standard strains were tested for fermentation of apple pectin as the main carbohydrate in selective broth media.
RESULTS: An increase in the growth of bacteria Bifidobacteria, Lactobacillus, and a consortium of bacteria selected from human milk and infant feces was noticed after pectin fermentation. It was found that pectin has a positive impact on the increase of the antioxidant capacity in all the tested samples. A high correlation was achieved, comparing electrochemical with spectrophotometric methods. Antimicrobial tests showed that pectin and fermentation products have slight or no influence at all on the growth of standard strains of Escherichia coli, Staphylococcus aureus and Candida albicans.
CONCLUSIONS: The results showed the importance of pectin as an antioxidant and its impact on the development of potential probiotic microbiota in mothers and infants. The study indicated a new potential application of pectin as a supplement in infant food.
PB  - Turin : Edizioni Minerva Medica
T2  - Minerva Biotechnology and Biomolecular Research
T1  - Investigation of pectin as a prebiotic, antioxidant and antimicrobial agent for the bacteria selected from human milk of mothers of premature infants
VL  - 33
IS  - 2
SP  - 86
EP  - 92
DO  - 10.23736/S2724-542X.21.02760-9
ER  - 

@article{
author = "Lugonja, Nikoleta and Lončarević, Branka D. and Stanković, Dalibor and Marinković, Vesna S. and Lješević, Marija and Spasić, Snežana and Beškoski, Vladimir",
year = "2021",
abstract = "BACKGROUND: Pectin is a complex polysaccharide, present in fresh and processed food as an additive, which also serves as an important source of fiber. In this work, the aim was to investigate in-vitro application of apple pectin as a possible antimicrobial, antioxidant and prebiotic agent for growth of bacteria selected from human milk and infant feces, as well as standard strains Lactobacillus rhamnosus and Bifidobacterium bifidum.
METHODS : All the bacteria isolated from the milk of mothers of preterm and term-born infants, bacteria from infant feces and standard strains were tested for fermentation of apple pectin as the main carbohydrate in selective broth media.
RESULTS: An increase in the growth of bacteria Bifidobacteria, Lactobacillus, and a consortium of bacteria selected from human milk and infant feces was noticed after pectin fermentation. It was found that pectin has a positive impact on the increase of the antioxidant capacity in all the tested samples. A high correlation was achieved, comparing electrochemical with spectrophotometric methods. Antimicrobial tests showed that pectin and fermentation products have slight or no influence at all on the growth of standard strains of Escherichia coli, Staphylococcus aureus and Candida albicans.
CONCLUSIONS: The results showed the importance of pectin as an antioxidant and its impact on the development of potential probiotic microbiota in mothers and infants. The study indicated a new potential application of pectin as a supplement in infant food.",
publisher = "Turin : Edizioni Minerva Medica",
journal = "Minerva Biotechnology and Biomolecular Research",
title = "Investigation of pectin as a prebiotic, antioxidant and antimicrobial agent for the bacteria selected from human milk of mothers of premature infants",
volume = "33",
number = "2",
pages = "86-92",
doi = "10.23736/S2724-542X.21.02760-9"
}

Lugonja, N., Lončarević, B. D., Stanković, D., Marinković, V. S., Lješević, M., Spasić, S.,& Beškoski, V.. (2021). Investigation of pectin as a prebiotic, antioxidant and antimicrobial agent for the bacteria selected from human milk of mothers of premature infants. in Minerva Biotechnology and Biomolecular Research
Turin : Edizioni Minerva Medica., 33(2), 86-92.
https://doi.org/10.23736/S2724-542X.21.02760-9

Lugonja N, Lončarević BD, Stanković D, Marinković VS, Lješević M, Spasić S, Beškoski V. Investigation of pectin as a prebiotic, antioxidant and antimicrobial agent for the bacteria selected from human milk of mothers of premature infants. in Minerva Biotechnology and Biomolecular Research. 2021;33(2):86-92.
doi:10.23736/S2724-542X.21.02760-9 .

Lugonja, Nikoleta, Lončarević, Branka D., Stanković, Dalibor, Marinković, Vesna S., Lješević, Marija, Spasić, Snežana, Beškoski, Vladimir, "Investigation of pectin as a prebiotic, antioxidant and antimicrobial agent for the bacteria selected from human milk of mothers of premature infants" in Minerva Biotechnology and Biomolecular Research, 33, no. 2 (2021):86-92,
https://doi.org/10.23736/S2724-542X.21.02760-9 . .

TY  - JOUR
AU  - Milenković, Ivana
AU  - Radotić, Ksenija
AU  - Despotović, Jovana
AU  - Lončarević, Branka D.
AU  - Lješević, Marija
AU  - Spasić, Slađana Z.
AU  - Nikolić, Aleksandra
AU  - Beškoski, Vladimir
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0166445X21001260
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4566
AB  - Cerium oxide nanoparticles (nCeO2) have widespread applications, but they can be hazardous to the environment. Some reports indicate the toxic effect of nCeO2 on tested animals, but literature data are mainly contradictory. Coating of nCeO2 can improve their suspension stability and change their interaction with the environment, which can consequently decrease their toxic effects. Herein, the exopolysaccharides levan and pullulan, due to their high water solubility, biocompatibility, and ability to form film, were used to coat nCeO2. Additionally, the monosaccharide glucose was used, since it is a common material for nanoparticle coating. This is the first study investigating the impact of carbohydrate-coated nCeO2 in comparison to uncoated nCeO2 using different model organisms. The aim of this study was to test the acute toxicity of carbohydrate-coated nCeO2 on the bacterium Vibrio fischeri NRRL B-11177, the crustacean Daphnia magna, and zebrafish Danio rerio. The second aim was to investigate the effects of nCeO2 on respiration in Daphnia magna which was performed for the first time. Finally, it was important to see the relation between Ce bioaccumulation in Daphnia magna and Danio rerio and other investigated parameters. Our results revealed that the coating decreased the toxicity of nCeO2 on Vibrio fischeri. The coating of nCeO2 did not affect the nanoparticles’ accumulation/adsorption or mortality in Daphnia magna or Danio rerio. Monitoring of respiration in Daphnia magna revealed changes in CO2 production after exposure to coated nCeO2, while the crustacean's O2 consumption was not affected by any of the coated nCeO2. In summary, this study revealed that, at 200 mg L−1, uncoated and carbohydrate-coated nCeO2 are not toxic for the tested organisms, however, the CO2 production in Daphnia magna is different when they are treated with coated and uncoated nCeO2. The highest production was in glucose and levan-coated nCeO2 according to their highest suspension stability. Daphnia magna (D. magna), Danio rerio (D. rerio), Vibrio fischeri (V. fischeri)
PB  - Elsevier
T2  - Aquatic Toxicology
T1  - Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio
VL  - 236
SP  - 105867
DO  - 10.1016/j.aquatox.2021.105867
ER  - 

@article{
author = "Milenković, Ivana and Radotić, Ksenija and Despotović, Jovana and Lončarević, Branka D. and Lješević, Marija and Spasić, Slađana Z. and Nikolić, Aleksandra and Beškoski, Vladimir",
year = "2021",
abstract = "Cerium oxide nanoparticles (nCeO2) have widespread applications, but they can be hazardous to the environment. Some reports indicate the toxic effect of nCeO2 on tested animals, but literature data are mainly contradictory. Coating of nCeO2 can improve their suspension stability and change their interaction with the environment, which can consequently decrease their toxic effects. Herein, the exopolysaccharides levan and pullulan, due to their high water solubility, biocompatibility, and ability to form film, were used to coat nCeO2. Additionally, the monosaccharide glucose was used, since it is a common material for nanoparticle coating. This is the first study investigating the impact of carbohydrate-coated nCeO2 in comparison to uncoated nCeO2 using different model organisms. The aim of this study was to test the acute toxicity of carbohydrate-coated nCeO2 on the bacterium Vibrio fischeri NRRL B-11177, the crustacean Daphnia magna, and zebrafish Danio rerio. The second aim was to investigate the effects of nCeO2 on respiration in Daphnia magna which was performed for the first time. Finally, it was important to see the relation between Ce bioaccumulation in Daphnia magna and Danio rerio and other investigated parameters. Our results revealed that the coating decreased the toxicity of nCeO2 on Vibrio fischeri. The coating of nCeO2 did not affect the nanoparticles’ accumulation/adsorption or mortality in Daphnia magna or Danio rerio. Monitoring of respiration in Daphnia magna revealed changes in CO2 production after exposure to coated nCeO2, while the crustacean's O2 consumption was not affected by any of the coated nCeO2. In summary, this study revealed that, at 200 mg L−1, uncoated and carbohydrate-coated nCeO2 are not toxic for the tested organisms, however, the CO2 production in Daphnia magna is different when they are treated with coated and uncoated nCeO2. The highest production was in glucose and levan-coated nCeO2 according to their highest suspension stability. Daphnia magna (D. magna), Danio rerio (D. rerio), Vibrio fischeri (V. fischeri)",
publisher = "Elsevier",
journal = "Aquatic Toxicology",
title = "Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio",
volume = "236",
pages = "105867",
doi = "10.1016/j.aquatox.2021.105867"
}

Milenković, I., Radotić, K., Despotović, J., Lončarević, B. D., Lješević, M., Spasić, S. Z., Nikolić, A.,& Beškoski, V.. (2021). Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio. in Aquatic Toxicology
Elsevier., 236, 105867.
https://doi.org/10.1016/j.aquatox.2021.105867

Milenković I, Radotić K, Despotović J, Lončarević BD, Lješević M, Spasić SZ, Nikolić A, Beškoski V. Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio. in Aquatic Toxicology. 2021;236:105867.
doi:10.1016/j.aquatox.2021.105867 .

Milenković, Ivana, Radotić, Ksenija, Despotović, Jovana, Lončarević, Branka D., Lješević, Marija, Spasić, Slađana Z., Nikolić, Aleksandra, Beškoski, Vladimir, "Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio" in Aquatic Toxicology, 236 (2021):105867,
https://doi.org/10.1016/j.aquatox.2021.105867 . .

TY  - CONF
AU  - Beškoski, Vladimir
AU  - Munćan, J.
AU  - Lončarević, Branka D.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Jakovljević, Dragica
AU  - Inui, H.
AU  - Tsenkova, R.
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5235
AB  - Aquaphotomics is a relatively novel scientific discipline which can be used for elucidation of structural and  related functional properties of aqueous systems. The aim of this study was to analyse water solutions of carbohydrates of different size from monosaccharides (glucose and fructose) via, disaccharides (sucrose), gluco and fructo-oligosaccharides and various selected polisaccharides (based on glucose and fructose, respectively) using Aquaphotomics. Knowing that main functions of carbohydrates within our body are various: providing energy and regulation of blood glucose; sparing the use of proteins for energy; breakdown of fatty acids and preventing ketosis; biological recognition processes; flavor and sweeteners and dietary fibers who can promote digestive health, knowledge about their interaction with water molecules can have strong influence on our understanding of some basic but also complex metabolic processes.
PB  - Aquaphotomics International Society
C3  - Proceedings of the 4th Aquaphotomics International Conference March 20 – 22, 2021, Hybrid Event - Kobe University, Centennial Hall (Rokko Hall)
T1  - Can carbohydrates change the shape of water?
SP  - 125
EP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5235
ER  - 

@conference{
author = "Beškoski, Vladimir and Munćan, J. and Lončarević, Branka D. and Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica and Inui, H. and Tsenkova, R.",
year = "2021",
abstract = "Aquaphotomics is a relatively novel scientific discipline which can be used for elucidation of structural and  related functional properties of aqueous systems. The aim of this study was to analyse water solutions of carbohydrates of different size from monosaccharides (glucose and fructose) via, disaccharides (sucrose), gluco and fructo-oligosaccharides and various selected polisaccharides (based on glucose and fructose, respectively) using Aquaphotomics. Knowing that main functions of carbohydrates within our body are various: providing energy and regulation of blood glucose; sparing the use of proteins for energy; breakdown of fatty acids and preventing ketosis; biological recognition processes; flavor and sweeteners and dietary fibers who can promote digestive health, knowledge about their interaction with water molecules can have strong influence on our understanding of some basic but also complex metabolic processes.",
publisher = "Aquaphotomics International Society",
journal = "Proceedings of the 4th Aquaphotomics International Conference March 20 – 22, 2021, Hybrid Event - Kobe University, Centennial Hall (Rokko Hall)",
title = "Can carbohydrates change the shape of water?",
pages = "125-126",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5235"
}

Beškoski, V., Munćan, J., Lončarević, B. D., Gojgić-Cvijović, G. D., Jakovljević, D., Inui, H.,& Tsenkova, R.. (2021). Can carbohydrates change the shape of water?. in Proceedings of the 4th Aquaphotomics International Conference March 20 – 22, 2021, Hybrid Event - Kobe University, Centennial Hall (Rokko Hall)
Aquaphotomics International Society., 125-126.
https://hdl.handle.net/21.15107/rcub_cherry_5235

Beškoski V, Munćan J, Lončarević BD, Gojgić-Cvijović GD, Jakovljević D, Inui H, Tsenkova R. Can carbohydrates change the shape of water?. in Proceedings of the 4th Aquaphotomics International Conference March 20 – 22, 2021, Hybrid Event - Kobe University, Centennial Hall (Rokko Hall). 2021;:125-126.
https://hdl.handle.net/21.15107/rcub_cherry_5235 .

Beškoski, Vladimir, Munćan, J., Lončarević, Branka D., Gojgić-Cvijović, Gordana D., Jakovljević, Dragica, Inui, H., Tsenkova, R., "Can carbohydrates change the shape of water?" in Proceedings of the 4th Aquaphotomics International Conference March 20 – 22, 2021, Hybrid Event - Kobe University, Centennial Hall (Rokko Hall) (2021):125-126,
https://hdl.handle.net/21.15107/rcub_cherry_5235 .

TY  - THES
AU  - Lončarević, Branka D.
PY  - 2019
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6985
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:20475/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51644687
UR  - http://nardus.mpn.gov.rs/123456789/11668
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3900
AB  - U okviru ove doktorske disertacije proučavan je uticaj različitih uslova fermentacijena produkciju levana bakterije Bacillus lichenifomis NS032, ispitivana struktura dobijenogpolisaharida i sintetisani kalemljeni kopolimeri levana i polistirena (Lev-g-PS).U prvoj seriji eksperimenata optimizovane su podloge za produkciju levanatehnikom jedne promenljive u vremenu i metodom površine odgovora. Najviši prinosipolisaharida se dobijaju na temperaturi od 37 °C i bez mešanja. Upotrebom podloge sa196,75 g/L saharoze, 2,44 g/L amonijum-hlorida i inicijalnog pH od 6,97 dobija se 47,84g/L levana LS200, a na podlozi sa 397,65 g/L saharoze, 4,62 g/L amonijum-hlorida iinicijalnog pH od 7,31 dobija se 99,19 g/L levana LS400. U sledećim uslovima: 62,6 %melase, 4,66 g/L fosfata i inicijalnim pH od 7,2 dobija se 53,6 g/L levana LM.Strukturna ispitivanja (kisela hidroliza, metilaciona analiza, FTIR, 13C- i 1H-NMR,COSY, HSQC i HMBC) su pokazala da su dobijeni levani polifruktani sa β-(2,6)-glikozidnom vezom u osnovnom nizu i grananjem u položaju β-(2,1). Stepeni grananja su11,04 ± 0,57 %, 11,99 ± 0,63 % 12,68 ± 0,71 % za levane LS200, LS400 i LMrespektivno. Levani LS200 i LM relativno su visoke molekulske mase (opseg > 106 Da) saviskozitetima 0,25 dL/g i 0,24 dL/g, dok je levan LS400 niže molekulske mase (105 Da) iviskoziteta 0,14 dL/g.U poslednjoj seriji eksperimenata ispitivan je uticaj različitih reakcionih uslova nasintezu Lev-g-PS slobodnoradikalskim mehanizmom i upotrebom kalijum-persulfata kaoinicijatora. Maksimalni G (%) = 58,1 dobijen je na 70 °C, u atmosferi azota i upotrebomaminoaktivatora DMEA. FTIR, SEM, TG/DTA, XRD i 13C NMR spektroskopija dobijenogkopolimera su pokazali da je došlo do reakcije kalemljenja između levana i polistirena.
AB  - Within this doctoral dissertation, the effects of various fermentation factors on levanproduction by Bacillus lichenifomis NS032 were studied, structure of obtainedpolysaccharide was investigated and grafted copolymers of levan and polystyrene (Lev-g-PS) were synthetized.In the first series of experiments, media for levan production were optimized usingone factor at a time and response surface methods. The highest levan yield was obtained at37 °C and without agitation. Using the medium with 196.75 g/L sucrose, 2.44 g/Lammonium chloride and pH 6.97, obtained amount of LS200 levan was 47.84 g/L and inmedium with 397.65 g/L sucrose, 4.62 g/L ammonium chloride and pH 7.31 amount ofLS400 levan was 99.19 g/L. In the following conditions: 62.6 % molasses, 4.66 g/Lphosphate and an initial pH 7.2, LM levan (53.6 g/L) was obtained.Structural characterization (acid hydrolisis, methylation analysis, FTIR, 13C- and1H-NMR, COSY, HSQC i HMBC) showed that investigated levans are polyfructans with β-(2,6)- glycosidic linkages in backbone and branching through β-(2,1) positions. The extentsof branching are 11.04 ± 0.57 %, 11.99 ± 0.63 % 12.68 ± 0.71 % for LS200, LS400 andLM levans, respectively. LS200 and LM have relatively high molecular weight (range >106 Da) and viscosity (0.25 dL/g and 0.24 dL/g), contrary to LS400 with lower molecularweight (105 Da) and viscosity (0.14 dL/g).Finally, influence of different reaction conditions on synthesis of Lev-g-PS by freeradical mechanism and potassium persulfate as initiator was investigated. Maximum G (%)= 58.1 was obtained at 70 °C, in nitrogen atmosphere and using the amine activator DMEA.FTIR, SEM, TG/DTA, XRD and 13C NMR spectroscopy of copolymer showed thatgrafting reaction of levan and polystyrene was successful.
PB  - Универзитет у Београду, Хемијски факултет
T2  - Универзитет у Београду
T1  - Optimizacija produkcije levana bakterije Bacillus licheniformis i primena u sintezi kopolimera
UR  - https://hdl.handle.net/21.15107/rcub_nardus_11668
ER  - 

@phdthesis{
author = "Lončarević, Branka D.",
year = "2019",
abstract = "U okviru ove doktorske disertacije proučavan je uticaj različitih uslova fermentacijena produkciju levana bakterije Bacillus lichenifomis NS032, ispitivana struktura dobijenogpolisaharida i sintetisani kalemljeni kopolimeri levana i polistirena (Lev-g-PS).U prvoj seriji eksperimenata optimizovane su podloge za produkciju levanatehnikom jedne promenljive u vremenu i metodom površine odgovora. Najviši prinosipolisaharida se dobijaju na temperaturi od 37 °C i bez mešanja. Upotrebom podloge sa196,75 g/L saharoze, 2,44 g/L amonijum-hlorida i inicijalnog pH od 6,97 dobija se 47,84g/L levana LS200, a na podlozi sa 397,65 g/L saharoze, 4,62 g/L amonijum-hlorida iinicijalnog pH od 7,31 dobija se 99,19 g/L levana LS400. U sledećim uslovima: 62,6 %melase, 4,66 g/L fosfata i inicijalnim pH od 7,2 dobija se 53,6 g/L levana LM.Strukturna ispitivanja (kisela hidroliza, metilaciona analiza, FTIR, 13C- i 1H-NMR,COSY, HSQC i HMBC) su pokazala da su dobijeni levani polifruktani sa β-(2,6)-glikozidnom vezom u osnovnom nizu i grananjem u položaju β-(2,1). Stepeni grananja su11,04 ± 0,57 %, 11,99 ± 0,63 % 12,68 ± 0,71 % za levane LS200, LS400 i LMrespektivno. Levani LS200 i LM relativno su visoke molekulske mase (opseg > 106 Da) saviskozitetima 0,25 dL/g i 0,24 dL/g, dok je levan LS400 niže molekulske mase (105 Da) iviskoziteta 0,14 dL/g.U poslednjoj seriji eksperimenata ispitivan je uticaj različitih reakcionih uslova nasintezu Lev-g-PS slobodnoradikalskim mehanizmom i upotrebom kalijum-persulfata kaoinicijatora. Maksimalni G (%) = 58,1 dobijen je na 70 °C, u atmosferi azota i upotrebomaminoaktivatora DMEA. FTIR, SEM, TG/DTA, XRD i 13C NMR spektroskopija dobijenogkopolimera su pokazali da je došlo do reakcije kalemljenja između levana i polistirena., Within this doctoral dissertation, the effects of various fermentation factors on levanproduction by Bacillus lichenifomis NS032 were studied, structure of obtainedpolysaccharide was investigated and grafted copolymers of levan and polystyrene (Lev-g-PS) were synthetized.In the first series of experiments, media for levan production were optimized usingone factor at a time and response surface methods. The highest levan yield was obtained at37 °C and without agitation. Using the medium with 196.75 g/L sucrose, 2.44 g/Lammonium chloride and pH 6.97, obtained amount of LS200 levan was 47.84 g/L and inmedium with 397.65 g/L sucrose, 4.62 g/L ammonium chloride and pH 7.31 amount ofLS400 levan was 99.19 g/L. In the following conditions: 62.6 % molasses, 4.66 g/Lphosphate and an initial pH 7.2, LM levan (53.6 g/L) was obtained.Structural characterization (acid hydrolisis, methylation analysis, FTIR, 13C- and1H-NMR, COSY, HSQC i HMBC) showed that investigated levans are polyfructans with β-(2,6)- glycosidic linkages in backbone and branching through β-(2,1) positions. The extentsof branching are 11.04 ± 0.57 %, 11.99 ± 0.63 % 12.68 ± 0.71 % for LS200, LS400 andLM levans, respectively. LS200 and LM have relatively high molecular weight (range >106 Da) and viscosity (0.25 dL/g and 0.24 dL/g), contrary to LS400 with lower molecularweight (105 Da) and viscosity (0.14 dL/g).Finally, influence of different reaction conditions on synthesis of Lev-g-PS by freeradical mechanism and potassium persulfate as initiator was investigated. Maximum G (%)= 58.1 was obtained at 70 °C, in nitrogen atmosphere and using the amine activator DMEA.FTIR, SEM, TG/DTA, XRD and 13C NMR spectroscopy of copolymer showed thatgrafting reaction of levan and polystyrene was successful.",
publisher = "Универзитет у Београду, Хемијски факултет",
journal = "Универзитет у Београду",
title = "Optimizacija produkcije levana bakterije Bacillus licheniformis i primena u sintezi kopolimera",
url = "https://hdl.handle.net/21.15107/rcub_nardus_11668"
}

Lončarević, B. D.. (2019). Optimizacija produkcije levana bakterije Bacillus licheniformis i primena u sintezi kopolimera. in Универзитет у Београду
Универзитет у Београду, Хемијски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_11668

Lončarević BD. Optimizacija produkcije levana bakterije Bacillus licheniformis i primena u sintezi kopolimera. in Универзитет у Београду. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_11668 .

Lončarević, Branka D., "Optimizacija produkcije levana bakterije Bacillus licheniformis i primena u sintezi kopolimera" in Универзитет у Београду (2019),
https://hdl.handle.net/21.15107/rcub_nardus_11668 .

TY  - CONF
AU  - Lončarević, Branka D.
AU  - Lješević, Marija
AU  - Bubanja, Itana Nuša
AU  - Beškoski, Vladimir
AU  - Gojgić-Cvijović, Gordana D.
AU  - Velikić, Zoran
AU  - Stanisavljev, Dragomir
PY  - 2019
UR  - https://www.shd.org.rs/index.php/proceedings-56
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4951
AB  - The analysis of the electric, magnetic and electromagnetic fields influence on microorganisms is a very popular research topic, since these fields could potentially act as stressors and affect the microbial metabolism and survival. The aim of this work was to investigate the influence of the low frequency magnetic field (MF) with scan regime from 10 Hz to 50 Hz on S. cerevisiae respiration. The experiment was performed in five replicates and monitored using the Micro-Oxymax® respirometer. All five experiments showed lower cumulative O2 consumption in MF exposed samples, compared to the control sample and inconsistent cumulative CO2 production. However, these differences in O2 consumption and CO2 production were statistically significant. Even though additional experiments are necessary, these results strongly suggest that this is a good basis for further investigation in this field.
AB  - Ispitivanje uticaja električnog, magnetnog i elektromagnetnog polja na mikroorganizme jeveoma aktuelni predmet istrazivanja, jer ova fizička polja potencijalno deluju kao faktoristresa i tako utiču na mikrobni metabolizam, ponašanje i preživljavanje. U ovom raduispitivan je uticaj niskofrekventnog magnetnog polja (MP) sa konstantnim intervalomskeniranja od 10 do 50 Hz na respiraciju ćelija kvasca, S. cerevisiae. Eksperiment je rađen upet ponavljanja i praćen Micro-Oxymax® respirometrom. Kumulativna potrošnja kiseonikaje bila manja kod ćelija izloženih MP u svih pet ponavljanja, dok je produkcija CO2 bilanekonzistentna. Međutim, ove razlike u potrošnji O2 i produkciji CO2 su statistički značajne.Iako su dodatna ispitivanja neophodna, dobijeni rezultati ovih inicijalnih eksperimenatapredstavljaju dobru osnovu za dalja istraživanja u ovoj oblasti.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 56th Meeting of the Serbian chemical Society - Proceedings / 56. Savetovanje Srpskog hemijskog društva - Knjiga radova Niš 7-8.9. 2019.
T1  - The influence of the low frequency magnetic field with scan regime from 10 Hz to 50 Hz on Saccharomyces cerevisiae respiration
T1  - Uticaj niskofrekventnog magnetnog polja (10-50 Hz) na respiracionu aktivnost ćelija kvasca Saccharomyces cerevisiae
SP  - 24
EP  - 29
UR  - https://hdl.handle.net/21.15107/rcub_cer_3289
ER  - 

@conference{
author = "Lončarević, Branka D. and Lješević, Marija and Bubanja, Itana Nuša and Beškoski, Vladimir and Gojgić-Cvijović, Gordana D. and Velikić, Zoran and Stanisavljev, Dragomir",
year = "2019",
abstract = "The analysis of the electric, magnetic and electromagnetic fields influence on microorganisms is a very popular research topic, since these fields could potentially act as stressors and affect the microbial metabolism and survival. The aim of this work was to investigate the influence of the low frequency magnetic field (MF) with scan regime from 10 Hz to 50 Hz on S. cerevisiae respiration. The experiment was performed in five replicates and monitored using the Micro-Oxymax® respirometer. All five experiments showed lower cumulative O2 consumption in MF exposed samples, compared to the control sample and inconsistent cumulative CO2 production. However, these differences in O2 consumption and CO2 production were statistically significant. Even though additional experiments are necessary, these results strongly suggest that this is a good basis for further investigation in this field., Ispitivanje uticaja električnog, magnetnog i elektromagnetnog polja na mikroorganizme jeveoma aktuelni predmet istrazivanja, jer ova fizička polja potencijalno deluju kao faktoristresa i tako utiču na mikrobni metabolizam, ponašanje i preživljavanje. U ovom raduispitivan je uticaj niskofrekventnog magnetnog polja (MP) sa konstantnim intervalomskeniranja od 10 do 50 Hz na respiraciju ćelija kvasca, S. cerevisiae. Eksperiment je rađen upet ponavljanja i praćen Micro-Oxymax® respirometrom. Kumulativna potrošnja kiseonikaje bila manja kod ćelija izloženih MP u svih pet ponavljanja, dok je produkcija CO2 bilanekonzistentna. Međutim, ove razlike u potrošnji O2 i produkciji CO2 su statistički značajne.Iako su dodatna ispitivanja neophodna, dobijeni rezultati ovih inicijalnih eksperimenatapredstavljaju dobru osnovu za dalja istraživanja u ovoj oblasti.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "56th Meeting of the Serbian chemical Society - Proceedings / 56. Savetovanje Srpskog hemijskog društva - Knjiga radova Niš 7-8.9. 2019.",
title = "The influence of the low frequency magnetic field with scan regime from 10 Hz to 50 Hz on Saccharomyces cerevisiae respiration, Uticaj niskofrekventnog magnetnog polja (10-50 Hz) na respiracionu aktivnost ćelija kvasca Saccharomyces cerevisiae",
pages = "24-29",
url = "https://hdl.handle.net/21.15107/rcub_cer_3289"
}

Lončarević, B. D., Lješević, M., Bubanja, I. N., Beškoski, V., Gojgić-Cvijović, G. D., Velikić, Z.,& Stanisavljev, D.. (2019). The influence of the low frequency magnetic field with scan regime from 10 Hz to 50 Hz on Saccharomyces cerevisiae respiration. in 56th Meeting of the Serbian chemical Society - Proceedings / 56. Savetovanje Srpskog hemijskog društva - Knjiga radova Niš 7-8.9. 2019.
Beograd : Srpsko hemijsko društvo., 24-29.
https://hdl.handle.net/21.15107/rcub_cer_3289

Lončarević BD, Lješević M, Bubanja IN, Beškoski V, Gojgić-Cvijović GD, Velikić Z, Stanisavljev D. The influence of the low frequency magnetic field with scan regime from 10 Hz to 50 Hz on Saccharomyces cerevisiae respiration. in 56th Meeting of the Serbian chemical Society - Proceedings / 56. Savetovanje Srpskog hemijskog društva - Knjiga radova Niš 7-8.9. 2019.. 2019;:24-29.
https://hdl.handle.net/21.15107/rcub_cer_3289 .

Lončarević, Branka D., Lješević, Marija, Bubanja, Itana Nuša, Beškoski, Vladimir, Gojgić-Cvijović, Gordana D., Velikić, Zoran, Stanisavljev, Dragomir, "The influence of the low frequency magnetic field with scan regime from 10 Hz to 50 Hz on Saccharomyces cerevisiae respiration" in 56th Meeting of the Serbian chemical Society - Proceedings / 56. Savetovanje Srpskog hemijskog društva - Knjiga radova Niš 7-8.9. 2019. (2019):24-29,
https://hdl.handle.net/21.15107/rcub_cer_3289 .

TY  - CONF
AU  - Lončarević, Branka D.
AU  - Lješević, Marija
AU  - Bubanja, Itana Nuša
AU  - Beškoski, Vladimir
AU  - Gojgić-Cvijović, Gordana D.
AU  - Velikić, Zoran
AU  - Stanisavljev, Dragomir
PY  - 2019
UR  - https://www.shd.org.rs/index.php/abstracts-56
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4952
AB  - The analysis of the electric, magnetic and electromagnetic fields influence on microorganisms is a very popular research topic, since these fields could potentially act as stressors and affect the microbial metabolism and survival. The aim of this work was to investigate the influence of the low frequency magnetic field (MF) with scan regime from 10 Hz to 50 Hz on S. cerevisiae respiration. The experiment was performed in five replicates and monitored using the Micro-Oxymax® respirometer. All five experiments showed lower cumulative O2 consumption in MF exposed samples, compared to the control sample and inconsistent cumulative CO2 production. However, these differences in O2 consumption and CO2 production were statistically significant. Even though additional experiments are necessary, these results strongly suggest that this is a good basis for further investigation in this field.
AB  - Ispitivanje uticaja električnog, magnetnog i elektromagnetnog polja na mikroorganizme je veoma aktuelni predmet istrazivanja, jer ova fizička polja potencijalno deluju kao faktori stresa i tako utiču na mikrobni metabolizam i preživljavanje. U ovom radu ispitivan je uticaj niskofrekventnog magnetnog polja (MP) sa konstantnim intervalom skeniranja od 10 do 50 Hz na respiraciju ćelija kvasca, S. cerevisiae. Eksperiment je rađen u pet ponavljanja i praćen Micro-Oxymax® respirometrom. Kumulativna potrošnja kiseonika je bila manja kod ćelija izloženih MP u svih pet ponavljanja, dok je produkcija CO2 bila nekonzistentna. Međutim, ove razlike u potrošnji O2 i produkciji CO2 su statistički značajne. Iako su dodatna ispitivanja neophodna, dobijeni rezultati ovih inicijalnih eksperimenata predstavljaju dobru osnovu za dalja istraživanja u ovoj oblasti.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 56th Meeting of the Serbian chemical Society - Book of Abstracts / 56. Savetovanje Srpskog hemijskog društva - Kratki izvodi radova Niš 7-8.9. 2019.
T1  - Influence of the low frequency magnetic field with scan regime from 10 Hz to 50 Hz on Saccharomyces cerevisiae respiration
T1  - Uticaj niskofrekventnog magnetnog polja (10-50 Hz) na respiracionu aktivnost ćelija kvasca Saccharomyces cerevisiae
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_cer_3288
ER  - 

@conference{
author = "Lončarević, Branka D. and Lješević, Marija and Bubanja, Itana Nuša and Beškoski, Vladimir and Gojgić-Cvijović, Gordana D. and Velikić, Zoran and Stanisavljev, Dragomir",
year = "2019",
abstract = "The analysis of the electric, magnetic and electromagnetic fields influence on microorganisms is a very popular research topic, since these fields could potentially act as stressors and affect the microbial metabolism and survival. The aim of this work was to investigate the influence of the low frequency magnetic field (MF) with scan regime from 10 Hz to 50 Hz on S. cerevisiae respiration. The experiment was performed in five replicates and monitored using the Micro-Oxymax® respirometer. All five experiments showed lower cumulative O2 consumption in MF exposed samples, compared to the control sample and inconsistent cumulative CO2 production. However, these differences in O2 consumption and CO2 production were statistically significant. Even though additional experiments are necessary, these results strongly suggest that this is a good basis for further investigation in this field., Ispitivanje uticaja električnog, magnetnog i elektromagnetnog polja na mikroorganizme je veoma aktuelni predmet istrazivanja, jer ova fizička polja potencijalno deluju kao faktori stresa i tako utiču na mikrobni metabolizam i preživljavanje. U ovom radu ispitivan je uticaj niskofrekventnog magnetnog polja (MP) sa konstantnim intervalom skeniranja od 10 do 50 Hz na respiraciju ćelija kvasca, S. cerevisiae. Eksperiment je rađen u pet ponavljanja i praćen Micro-Oxymax® respirometrom. Kumulativna potrošnja kiseonika je bila manja kod ćelija izloženih MP u svih pet ponavljanja, dok je produkcija CO2 bila nekonzistentna. Međutim, ove razlike u potrošnji O2 i produkciji CO2 su statistički značajne. Iako su dodatna ispitivanja neophodna, dobijeni rezultati ovih inicijalnih eksperimenata predstavljaju dobru osnovu za dalja istraživanja u ovoj oblasti.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "56th Meeting of the Serbian chemical Society - Book of Abstracts / 56. Savetovanje Srpskog hemijskog društva - Kratki izvodi radova Niš 7-8.9. 2019.",
title = "Influence of the low frequency magnetic field with scan regime from 10 Hz to 50 Hz on Saccharomyces cerevisiae respiration, Uticaj niskofrekventnog magnetnog polja (10-50 Hz) na respiracionu aktivnost ćelija kvasca Saccharomyces cerevisiae",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_cer_3288"
}

Lončarević, B. D., Lješević, M., Bubanja, I. N., Beškoski, V., Gojgić-Cvijović, G. D., Velikić, Z.,& Stanisavljev, D.. (2019). Influence of the low frequency magnetic field with scan regime from 10 Hz to 50 Hz on Saccharomyces cerevisiae respiration. in 56th Meeting of the Serbian chemical Society - Book of Abstracts / 56. Savetovanje Srpskog hemijskog društva - Kratki izvodi radova Niš 7-8.9. 2019.
Beograd : Srpsko hemijsko društvo., 8.
https://hdl.handle.net/21.15107/rcub_cer_3288

Lončarević BD, Lješević M, Bubanja IN, Beškoski V, Gojgić-Cvijović GD, Velikić Z, Stanisavljev D. Influence of the low frequency magnetic field with scan regime from 10 Hz to 50 Hz on Saccharomyces cerevisiae respiration. in 56th Meeting of the Serbian chemical Society - Book of Abstracts / 56. Savetovanje Srpskog hemijskog društva - Kratki izvodi radova Niš 7-8.9. 2019.. 2019;:8.
https://hdl.handle.net/21.15107/rcub_cer_3288 .

Lončarević, Branka D., Lješević, Marija, Bubanja, Itana Nuša, Beškoski, Vladimir, Gojgić-Cvijović, Gordana D., Velikić, Zoran, Stanisavljev, Dragomir, "Influence of the low frequency magnetic field with scan regime from 10 Hz to 50 Hz on Saccharomyces cerevisiae respiration" in 56th Meeting of the Serbian chemical Society - Book of Abstracts / 56. Savetovanje Srpskog hemijskog društva - Kratki izvodi radova Niš 7-8.9. 2019. (2019):8,
https://hdl.handle.net/21.15107/rcub_cer_3288 .

TY  - CONF
AU  - Beškoski, Vladimir
AU  - Lješević, Marija
AU  - Lončarević, Branka D.
AU  - Troncozo, M. I.
AU  - Balatti, P.A.
AU  - Saparrat, M.C.N.
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5236
AB  - The main objective of this paper was to analyze the transformation of grape pomace by a fungal strain isolated from leaf-litter and assess if it can be a successful pretreatment method for the grape pomace waste.
PB  - Association of chemistry and environment
C3  - 20th European Meeting on Environmental Chemistry, Association of Chemistry and the environment, Lodz, Poland, December 2-5, 2019
T1  - From environmental burden to valuable resource- a fungal reduction of phytotoxicity of grape pomace waste
SP  - 164
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5236
ER  - 

@conference{
author = "Beškoski, Vladimir and Lješević, Marija and Lončarević, Branka D. and Troncozo, M. I. and Balatti, P.A. and Saparrat, M.C.N.",
year = "2019",
abstract = "The main objective of this paper was to analyze the transformation of grape pomace by a fungal strain isolated from leaf-litter and assess if it can be a successful pretreatment method for the grape pomace waste.",
publisher = "Association of chemistry and environment",
journal = "20th European Meeting on Environmental Chemistry, Association of Chemistry and the environment, Lodz, Poland, December 2-5, 2019",
title = "From environmental burden to valuable resource- a fungal reduction of phytotoxicity of grape pomace waste",
pages = "164",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5236"
}

Beškoski, V., Lješević, M., Lončarević, B. D., Troncozo, M. I., Balatti, P.A.,& Saparrat, M.C.N.. (2019). From environmental burden to valuable resource- a fungal reduction of phytotoxicity of grape pomace waste. in 20th European Meeting on Environmental Chemistry, Association of Chemistry and the environment, Lodz, Poland, December 2-5, 2019
Association of chemistry and environment., 164.
https://hdl.handle.net/21.15107/rcub_cherry_5236

Beškoski V, Lješević M, Lončarević BD, Troncozo MI, Balatti P, Saparrat M. From environmental burden to valuable resource- a fungal reduction of phytotoxicity of grape pomace waste. in 20th European Meeting on Environmental Chemistry, Association of Chemistry and the environment, Lodz, Poland, December 2-5, 2019. 2019;:164.
https://hdl.handle.net/21.15107/rcub_cherry_5236 .

Beškoski, Vladimir, Lješević, Marija, Lončarević, Branka D., Troncozo, M. I., Balatti, P.A., Saparrat, M.C.N., "From environmental burden to valuable resource- a fungal reduction of phytotoxicity of grape pomace waste" in 20th European Meeting on Environmental Chemistry, Association of Chemistry and the environment, Lodz, Poland, December 2-5, 2019 (2019):164,
https://hdl.handle.net/21.15107/rcub_cherry_5236 .

TY  - JOUR
AU  - Bubanja, Itana Nuša
AU  - Lončarević, Branka D.
AU  - Lješević, Marija
AU  - Beškoski, Vladimir
AU  - Gojgić-Cvijović, Gordana D.
AU  - Velikić, Zoran
AU  - Stanisavljev, Dragomir
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3322
AB  - The influence of four low-frequency magnetic field (MF) ranges 10–300 Hz, 10–100 Hz, 10–50 Hz and 50–100 Hz in scanning regime (all frequencies from selected range were scanned during 100 s repetitively during 24 h) on baker's yeast cells Saccharomyces cerevisiae was examined by continuous measurements of cumulative O2 consumption and cumulative CO2 production over 24 h with Micro-Oxymax® respirometer. Besides respiration activity, measurements of cell growth and glucose uptake were performed as well. Statistical analysis indicated that, among all investigated low-frequency MF ranges, range from 10 Hz to 50 Hz had the greatest influence to yeast cell respiration and cell growth. More precisely, for this region, paired two sample one-tail t-test showed statistically significant differences in cumulative O2 consumption, cumulative CO2 production and S. cerevisiae cell number. Moreover samples exposed to MF range from 10 Hz to 50 Hz showed the same behavior in all five replicates: lower cumulative O2 consumption, higher cumulative CO2 production and higher cell number compared to control sample. This could be important from the application aspect, in industry (food, feed, brewery etc.) and biotechnology, because changes in cells metabolism are not caused by chemical treatment.
T2  - Chemical Engineering and Processing - Process Intensification
T1  - The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth
VL  - 143
SP  - 1
EP  - 7
DO  - 10.1016/j.cep.2019.107593
ER  - 

@article{
author = "Bubanja, Itana Nuša and Lončarević, Branka D. and Lješević, Marija and Beškoski, Vladimir and Gojgić-Cvijović, Gordana D. and Velikić, Zoran and Stanisavljev, Dragomir",
year = "2019",
abstract = "The influence of four low-frequency magnetic field (MF) ranges 10–300 Hz, 10–100 Hz, 10–50 Hz and 50–100 Hz in scanning regime (all frequencies from selected range were scanned during 100 s repetitively during 24 h) on baker's yeast cells Saccharomyces cerevisiae was examined by continuous measurements of cumulative O2 consumption and cumulative CO2 production over 24 h with Micro-Oxymax® respirometer. Besides respiration activity, measurements of cell growth and glucose uptake were performed as well. Statistical analysis indicated that, among all investigated low-frequency MF ranges, range from 10 Hz to 50 Hz had the greatest influence to yeast cell respiration and cell growth. More precisely, for this region, paired two sample one-tail t-test showed statistically significant differences in cumulative O2 consumption, cumulative CO2 production and S. cerevisiae cell number. Moreover samples exposed to MF range from 10 Hz to 50 Hz showed the same behavior in all five replicates: lower cumulative O2 consumption, higher cumulative CO2 production and higher cell number compared to control sample. This could be important from the application aspect, in industry (food, feed, brewery etc.) and biotechnology, because changes in cells metabolism are not caused by chemical treatment.",
journal = "Chemical Engineering and Processing - Process Intensification",
title = "The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth",
volume = "143",
pages = "1-7",
doi = "10.1016/j.cep.2019.107593"
}

Bubanja, I. N., Lončarević, B. D., Lješević, M., Beškoski, V., Gojgić-Cvijović, G. D., Velikić, Z.,& Stanisavljev, D.. (2019). The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth. in Chemical Engineering and Processing - Process Intensification, 143, 1-7.
https://doi.org/10.1016/j.cep.2019.107593

Bubanja IN, Lončarević BD, Lješević M, Beškoski V, Gojgić-Cvijović GD, Velikić Z, Stanisavljev D. The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth. in Chemical Engineering and Processing - Process Intensification. 2019;143:1-7.
doi:10.1016/j.cep.2019.107593 .

Bubanja, Itana Nuša, Lončarević, Branka D., Lješević, Marija, Beškoski, Vladimir, Gojgić-Cvijović, Gordana D., Velikić, Zoran, Stanisavljev, Dragomir, "The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth" in Chemical Engineering and Processing - Process Intensification, 143 (2019):1-7,
https://doi.org/10.1016/j.cep.2019.107593 . .

TY  - JOUR
AU  - Gojgić-Cvijović, Gordana D.
AU  - Jakovljević, Dragica M.
AU  - Lončarević, Branka D.
AU  - Todorović, Nevena M.
AU  - Pergal, Marija V.
AU  - Ćirić, J.
AU  - Loos, K.
AU  - Beškoski, Vladimir
AU  - Vrvić, Miroslav M.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/350
AB  - The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100–140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 53.2 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 × 106 Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose. © 2018 Elsevier B.V.
T2  - International Journal of Biological Macromolecules
T1  - Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium
VL  - 121
SP  - 142
EP  - 151
DO  - 10.1016/j.ijbiomac.2018.10.019
ER  - 

@article{
author = "Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica M. and Lončarević, Branka D. and Todorović, Nevena M. and Pergal, Marija V. and Ćirić, J. and Loos, K. and Beškoski, Vladimir and Vrvić, Miroslav M.",
year = "2019",
abstract = "The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100–140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 53.2 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 × 106 Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose. © 2018 Elsevier B.V.",
journal = "International Journal of Biological Macromolecules",
title = "Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium",
volume = "121",
pages = "142-151",
doi = "10.1016/j.ijbiomac.2018.10.019"
}

Gojgić-Cvijović, G. D., Jakovljević, D. M., Lončarević, B. D., Todorović, N. M., Pergal, M. V., Ćirić, J., Loos, K., Beškoski, V.,& Vrvić, M. M.. (2019). Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium. in International Journal of Biological Macromolecules, 121, 142-151.
https://doi.org/10.1016/j.ijbiomac.2018.10.019

Gojgić-Cvijović GD, Jakovljević DM, Lončarević BD, Todorović NM, Pergal MV, Ćirić J, Loos K, Beškoski V, Vrvić MM. Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium. in International Journal of Biological Macromolecules. 2019;121:142-151.
doi:10.1016/j.ijbiomac.2018.10.019 .

Gojgić-Cvijović, Gordana D., Jakovljević, Dragica M., Lončarević, Branka D., Todorović, Nevena M., Pergal, Marija V., Ćirić, J., Loos, K., Beškoski, Vladimir, Vrvić, Miroslav M., "Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium" in International Journal of Biological Macromolecules, 121 (2019):142-151,
https://doi.org/10.1016/j.ijbiomac.2018.10.019 . .

TY  - JOUR
AU  - Gojgić-Cvijović, Gordana D.
AU  - Jakovljević, Dragica M.
AU  - Lončarević, Branka D.
AU  - Todorović, Nevena M.
AU  - Pergal, Marija V.
AU  - Ćirić, J.
AU  - Loos, K.
AU  - Beškoski, Vladimir
AU  - Vrvić, Miroslav M.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2799
AB  - The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100–140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 53.2 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 × 106 Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose. © 2018 Elsevier B.V.
T2  - International Journal of Biological Macromolecules
T1  - Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium
VL  - 121
SP  - 142
EP  - 151
DO  - 10.1016/j.ijbiomac.2018.10.019
ER  - 

@article{
author = "Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica M. and Lončarević, Branka D. and Todorović, Nevena M. and Pergal, Marija V. and Ćirić, J. and Loos, K. and Beškoski, Vladimir and Vrvić, Miroslav M.",
year = "2019",
abstract = "The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100–140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 53.2 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 × 106 Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose. © 2018 Elsevier B.V.",
journal = "International Journal of Biological Macromolecules",
title = "Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium",
volume = "121",
pages = "142-151",
doi = "10.1016/j.ijbiomac.2018.10.019"
}

Gojgić-Cvijović, G. D., Jakovljević, D. M., Lončarević, B. D., Todorović, N. M., Pergal, M. V., Ćirić, J., Loos, K., Beškoski, V.,& Vrvić, M. M.. (2019). Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium. in International Journal of Biological Macromolecules, 121, 142-151.
https://doi.org/10.1016/j.ijbiomac.2018.10.019

Gojgić-Cvijović GD, Jakovljević DM, Lončarević BD, Todorović NM, Pergal MV, Ćirić J, Loos K, Beškoski V, Vrvić MM. Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium. in International Journal of Biological Macromolecules. 2019;121:142-151.
doi:10.1016/j.ijbiomac.2018.10.019 .

Gojgić-Cvijović, Gordana D., Jakovljević, Dragica M., Lončarević, Branka D., Todorović, Nevena M., Pergal, Marija V., Ćirić, J., Loos, K., Beškoski, Vladimir, Vrvić, Miroslav M., "Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium" in International Journal of Biological Macromolecules, 121 (2019):142-151,
https://doi.org/10.1016/j.ijbiomac.2018.10.019 . .

TY  - DATA
AU  - Gojgić-Cvijović, Gordana D.
AU  - Jakovljević, Dragica M.
AU  - Lončarević, Branka D.
AU  - Todorović, Nevena M.
AU  - Pergal, Marija V.
AU  - Ćirić, J.
AU  - Loos, K.
AU  - Beškoski, Vladimir
AU  - Vrvić, Miroslav M.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2943
T2  - International Journal of Biological Macromolecules
T1  - Supplementary data for the article: Gojgic-Cvijovic, G. D.; Jakovljevic, D. M.; Loncarevic, B. D.; Todorovic, N. M.; Pergal, M. V.; Ciric, J.; Loos, K.; Beškoski, V.; Vrvić, M. M. Production of Levan by Bacillus Licheniformis NS032 in Sugar Beet Molasses-Based Medium. International Journal of Biological Macromolecules 2019, 121, 142–151. https://doi.org/10.1016/j.ijbiomac.2018.10.019
UR  - https://hdl.handle.net/21.15107/rcub_cherry_2943
ER  - 

@misc{
author = "Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica M. and Lončarević, Branka D. and Todorović, Nevena M. and Pergal, Marija V. and Ćirić, J. and Loos, K. and Beškoski, Vladimir and Vrvić, Miroslav M.",
year = "2019",
journal = "International Journal of Biological Macromolecules",
title = "Supplementary data for the article: Gojgic-Cvijovic, G. D.; Jakovljevic, D. M.; Loncarevic, B. D.; Todorovic, N. M.; Pergal, M. V.; Ciric, J.; Loos, K.; Beškoski, V.; Vrvić, M. M. Production of Levan by Bacillus Licheniformis NS032 in Sugar Beet Molasses-Based Medium. International Journal of Biological Macromolecules 2019, 121, 142–151. https://doi.org/10.1016/j.ijbiomac.2018.10.019",
url = "https://hdl.handle.net/21.15107/rcub_cherry_2943"
}

Gojgić-Cvijović, G. D., Jakovljević, D. M., Lončarević, B. D., Todorović, N. M., Pergal, M. V., Ćirić, J., Loos, K., Beškoski, V.,& Vrvić, M. M.. (2019). Supplementary data for the article: Gojgic-Cvijovic, G. D.; Jakovljevic, D. M.; Loncarevic, B. D.; Todorovic, N. M.; Pergal, M. V.; Ciric, J.; Loos, K.; Beškoski, V.; Vrvić, M. M. Production of Levan by Bacillus Licheniformis NS032 in Sugar Beet Molasses-Based Medium. International Journal of Biological Macromolecules 2019, 121, 142–151. https://doi.org/10.1016/j.ijbiomac.2018.10.019. in International Journal of Biological Macromolecules.
https://hdl.handle.net/21.15107/rcub_cherry_2943

Gojgić-Cvijović GD, Jakovljević DM, Lončarević BD, Todorović NM, Pergal MV, Ćirić J, Loos K, Beškoski V, Vrvić MM. Supplementary data for the article: Gojgic-Cvijovic, G. D.; Jakovljevic, D. M.; Loncarevic, B. D.; Todorovic, N. M.; Pergal, M. V.; Ciric, J.; Loos, K.; Beškoski, V.; Vrvić, M. M. Production of Levan by Bacillus Licheniformis NS032 in Sugar Beet Molasses-Based Medium. International Journal of Biological Macromolecules 2019, 121, 142–151. https://doi.org/10.1016/j.ijbiomac.2018.10.019. in International Journal of Biological Macromolecules. 2019;.
https://hdl.handle.net/21.15107/rcub_cherry_2943 .

Gojgić-Cvijović, Gordana D., Jakovljević, Dragica M., Lončarević, Branka D., Todorović, Nevena M., Pergal, Marija V., Ćirić, J., Loos, K., Beškoski, Vladimir, Vrvić, Miroslav M., "Supplementary data for the article: Gojgic-Cvijovic, G. D.; Jakovljevic, D. M.; Loncarevic, B. D.; Todorovic, N. M.; Pergal, M. V.; Ciric, J.; Loos, K.; Beškoski, V.; Vrvić, M. M. Production of Levan by Bacillus Licheniformis NS032 in Sugar Beet Molasses-Based Medium. International Journal of Biological Macromolecules 2019, 121, 142–151. https://doi.org/10.1016/j.ijbiomac.2018.10.019" in International Journal of Biological Macromolecules (2019),
https://hdl.handle.net/21.15107/rcub_cherry_2943 .

TY  - JOUR
AU  - Lončarević, Branka D.
AU  - Lješević, Marija
AU  - Marković, Marijana
AU  - Anđelković, Ivan
AU  - Gojgić-Cvijović, Gordana D.
AU  - Jakovljević, Dragica M.
AU  - Beškoski, Vladimir
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3158
AB  - Microbial polysaccharides, due to their unique physiochemical properties, have found application in the food industry, cosmetics, pharmacy and medicine. In the environment, microbes can use polysaccharides to alleviate the adverse effects of heavy metals in their close proximity. This adaptive property shows interesting potential for bioremediation. Herein, the effects of the exopolysaccharides (EPS) levan, produced by the bacterium Bacillus licheniformis NS032 and pullulan, produced by the fungus Aureobasidium pullulans CH-1 in the presence of copper (Cu2+) have been investigated for the first time on antioxidant enzyme activity, respiration and Cu2+ bioaccumulation of Daphnia magna as well as the bioluminescence of Vibrio fischeri. Both EPS decreased toxicity of Cu2+ in the acute test with D. magna. The activity of catalase (CAT) was significantly diminished after acute exposure to Cu2+ in comparison to treatments with Cu2+ and EPS, while in the prolonged acute exposure the CAT activity did not show statistically significant (P ≤ 0.05) differences between treatments with and without the EPS. According to ICP-MS results, during prolonged acute exposure of neonates, the bioaccumulation of Cu2+ in treatments without the EPS was 52.03 μg/g of biomass (wet), while in treatments with EPS, the bioaccumulation was lower by one order of magnitude. The respiration of neonates during acute exposure to Cu2+ with or without the EPS was monitored using the MicroOxymax respirometer, and the results show the EPS can positively effect the respiration. In the case of bacterial bioluminescence, the toxicity of Cu2+ decreased in treatments with EPS (30 min EC10) from 3.54 mg/L to 140.61 mg/L (levan) and 45.00 mg/L (pullulan). This study demonstrates protective effect of EPS against Cu2+ toxicity on D. magna and V. fischeri, and opens the door for further investigation of potential application of levan and pullulan in bioremediation of heavy metals and mitigation of their adverse effects in the aquatic environment.
PB  - Elsevier
T2  - Ecotoxicology and Environmental Safety
T1  - Microbial levan and pullulan as potential protective agents for reducing adverse effects of copper on Daphnia magna and Vibrio fischeri
VL  - 181
SP  - 187
EP  - 193
DO  - 10.1016/j.ecoenv.2019.06.002
ER  - 

@article{
author = "Lončarević, Branka D. and Lješević, Marija and Marković, Marijana and Anđelković, Ivan and Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica M. and Beškoski, Vladimir",
year = "2019",
abstract = "Microbial polysaccharides, due to their unique physiochemical properties, have found application in the food industry, cosmetics, pharmacy and medicine. In the environment, microbes can use polysaccharides to alleviate the adverse effects of heavy metals in their close proximity. This adaptive property shows interesting potential for bioremediation. Herein, the effects of the exopolysaccharides (EPS) levan, produced by the bacterium Bacillus licheniformis NS032 and pullulan, produced by the fungus Aureobasidium pullulans CH-1 in the presence of copper (Cu2+) have been investigated for the first time on antioxidant enzyme activity, respiration and Cu2+ bioaccumulation of Daphnia magna as well as the bioluminescence of Vibrio fischeri. Both EPS decreased toxicity of Cu2+ in the acute test with D. magna. The activity of catalase (CAT) was significantly diminished after acute exposure to Cu2+ in comparison to treatments with Cu2+ and EPS, while in the prolonged acute exposure the CAT activity did not show statistically significant (P ≤ 0.05) differences between treatments with and without the EPS. According to ICP-MS results, during prolonged acute exposure of neonates, the bioaccumulation of Cu2+ in treatments without the EPS was 52.03 μg/g of biomass (wet), while in treatments with EPS, the bioaccumulation was lower by one order of magnitude. The respiration of neonates during acute exposure to Cu2+ with or without the EPS was monitored using the MicroOxymax respirometer, and the results show the EPS can positively effect the respiration. In the case of bacterial bioluminescence, the toxicity of Cu2+ decreased in treatments with EPS (30 min EC10) from 3.54 mg/L to 140.61 mg/L (levan) and 45.00 mg/L (pullulan). This study demonstrates protective effect of EPS against Cu2+ toxicity on D. magna and V. fischeri, and opens the door for further investigation of potential application of levan and pullulan in bioremediation of heavy metals and mitigation of their adverse effects in the aquatic environment.",
publisher = "Elsevier",
journal = "Ecotoxicology and Environmental Safety",
title = "Microbial levan and pullulan as potential protective agents for reducing adverse effects of copper on Daphnia magna and Vibrio fischeri",
volume = "181",
pages = "187-193",
doi = "10.1016/j.ecoenv.2019.06.002"
}

Lončarević, B. D., Lješević, M., Marković, M., Anđelković, I., Gojgić-Cvijović, G. D., Jakovljević, D. M.,& Beškoski, V.. (2019). Microbial levan and pullulan as potential protective agents for reducing adverse effects of copper on Daphnia magna and Vibrio fischeri. in Ecotoxicology and Environmental Safety
Elsevier., 181, 187-193.
https://doi.org/10.1016/j.ecoenv.2019.06.002

Lončarević BD, Lješević M, Marković M, Anđelković I, Gojgić-Cvijović GD, Jakovljević DM, Beškoski V. Microbial levan and pullulan as potential protective agents for reducing adverse effects of copper on Daphnia magna and Vibrio fischeri. in Ecotoxicology and Environmental Safety. 2019;181:187-193.
doi:10.1016/j.ecoenv.2019.06.002 .

Lončarević, Branka D., Lješević, Marija, Marković, Marijana, Anđelković, Ivan, Gojgić-Cvijović, Gordana D., Jakovljević, Dragica M., Beškoski, Vladimir, "Microbial levan and pullulan as potential protective agents for reducing adverse effects of copper on Daphnia magna and Vibrio fischeri" in Ecotoxicology and Environmental Safety, 181 (2019):187-193,
https://doi.org/10.1016/j.ecoenv.2019.06.002 . .

TY  - DATA
AU  - Lončarević, Branka D.
AU  - Lješević, Marija
AU  - Marković, Marijana
AU  - Anđelković, Ivan
AU  - Gojgić-Cvijović, Gordana D.
AU  - Jakovljević, Dragica M.
AU  - Beškoski, Vladimir
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3159
PB  - Elsevier
T2  - Ecotoxicology and Environmental Safety
T1  - Supplementary material for the article: Lončarević, B.; Lješević, M.; Marković, M.; Anđelković, I.; Gojgić-Cvijović, G.; Jakovljević, D.; Beškoski, V. Microbial Levan and Pullulan as Potential Protective Agents for Reducing Adverse Effects of Copper on Daphnia Magna and Vibrio Fischeri. Ecotoxicology and Environmental Safety 2019, 181, 187–193. https://doi.org/10.1016/j.ecoenv.2019.06.002
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3159
ER  - 

@misc{
author = "Lončarević, Branka D. and Lješević, Marija and Marković, Marijana and Anđelković, Ivan and Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica M. and Beškoski, Vladimir",
year = "2019",
publisher = "Elsevier",
journal = "Ecotoxicology and Environmental Safety",
title = "Supplementary material for the article: Lončarević, B.; Lješević, M.; Marković, M.; Anđelković, I.; Gojgić-Cvijović, G.; Jakovljević, D.; Beškoski, V. Microbial Levan and Pullulan as Potential Protective Agents for Reducing Adverse Effects of Copper on Daphnia Magna and Vibrio Fischeri. Ecotoxicology and Environmental Safety 2019, 181, 187–193. https://doi.org/10.1016/j.ecoenv.2019.06.002",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3159"
}

Lončarević, B. D., Lješević, M., Marković, M., Anđelković, I., Gojgić-Cvijović, G. D., Jakovljević, D. M.,& Beškoski, V.. (2019). Supplementary material for the article: Lončarević, B.; Lješević, M.; Marković, M.; Anđelković, I.; Gojgić-Cvijović, G.; Jakovljević, D.; Beškoski, V. Microbial Levan and Pullulan as Potential Protective Agents for Reducing Adverse Effects of Copper on Daphnia Magna and Vibrio Fischeri. Ecotoxicology and Environmental Safety 2019, 181, 187–193. https://doi.org/10.1016/j.ecoenv.2019.06.002. in Ecotoxicology and Environmental Safety
Elsevier..
https://hdl.handle.net/21.15107/rcub_cherry_3159

Lončarević BD, Lješević M, Marković M, Anđelković I, Gojgić-Cvijović GD, Jakovljević DM, Beškoski V. Supplementary material for the article: Lončarević, B.; Lješević, M.; Marković, M.; Anđelković, I.; Gojgić-Cvijović, G.; Jakovljević, D.; Beškoski, V. Microbial Levan and Pullulan as Potential Protective Agents for Reducing Adverse Effects of Copper on Daphnia Magna and Vibrio Fischeri. Ecotoxicology and Environmental Safety 2019, 181, 187–193. https://doi.org/10.1016/j.ecoenv.2019.06.002. in Ecotoxicology and Environmental Safety. 2019;.
https://hdl.handle.net/21.15107/rcub_cherry_3159 .

Lončarević, Branka D., Lješević, Marija, Marković, Marijana, Anđelković, Ivan, Gojgić-Cvijović, Gordana D., Jakovljević, Dragica M., Beškoski, Vladimir, "Supplementary material for the article: Lončarević, B.; Lješević, M.; Marković, M.; Anđelković, I.; Gojgić-Cvijović, G.; Jakovljević, D.; Beškoski, V. Microbial Levan and Pullulan as Potential Protective Agents for Reducing Adverse Effects of Copper on Daphnia Magna and Vibrio Fischeri. Ecotoxicology and Environmental Safety 2019, 181, 187–193. https://doi.org/10.1016/j.ecoenv.2019.06.002" in Ecotoxicology and Environmental Safety (2019),
https://hdl.handle.net/21.15107/rcub_cherry_3159 .

TY  - JOUR
AU  - Bubanja, Itana Nuša
AU  - Lončarević, Branka D.
AU  - Lješević, Marija
AU  - Beškoski, Vladimir
AU  - Gojgić-Cvijović, Gordana D.
AU  - Velikić, Zoran
AU  - Stanisavljev, Dragomir
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3321
AB  - The influence of four low-frequency magnetic field (MF) ranges 10–300 Hz, 10–100 Hz, 10–50 Hz and 50–100 Hz in scanning regime (all frequencies from selected range were scanned during 100 s repetitively during 24 h) on baker's yeast cells Saccharomyces cerevisiae was examined by continuous measurements of cumulative O2 consumption and cumulative CO2 production over 24 h with Micro-Oxymax® respirometer. Besides respiration activity, measurements of cell growth and glucose uptake were performed as well. Statistical analysis indicated that, among all investigated low-frequency MF ranges, range from 10 Hz to 50 Hz had the greatest influence to yeast cell respiration and cell growth. More precisely, for this region, paired two sample one-tail t-test showed statistically significant differences in cumulative O2 consumption, cumulative CO2 production and S. cerevisiae cell number. Moreover samples exposed to MF range from 10 Hz to 50 Hz showed the same behavior in all five replicates: lower cumulative O2 consumption, higher cumulative CO2 production and higher cell number compared to control sample. This could be important from the application aspect, in industry (food, feed, brewery etc.) and biotechnology, because changes in cells metabolism are not caused by chemical treatment.
T2  - Chemical Engineering and Processing - Process Intensification
T1  - The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth
VL  - 143
SP  - 1
EP  - 7
DO  - 10.1016/j.cep.2019.107593
ER  - 

@article{
author = "Bubanja, Itana Nuša and Lončarević, Branka D. and Lješević, Marija and Beškoski, Vladimir and Gojgić-Cvijović, Gordana D. and Velikić, Zoran and Stanisavljev, Dragomir",
year = "2019",
abstract = "The influence of four low-frequency magnetic field (MF) ranges 10–300 Hz, 10–100 Hz, 10–50 Hz and 50–100 Hz in scanning regime (all frequencies from selected range were scanned during 100 s repetitively during 24 h) on baker's yeast cells Saccharomyces cerevisiae was examined by continuous measurements of cumulative O2 consumption and cumulative CO2 production over 24 h with Micro-Oxymax® respirometer. Besides respiration activity, measurements of cell growth and glucose uptake were performed as well. Statistical analysis indicated that, among all investigated low-frequency MF ranges, range from 10 Hz to 50 Hz had the greatest influence to yeast cell respiration and cell growth. More precisely, for this region, paired two sample one-tail t-test showed statistically significant differences in cumulative O2 consumption, cumulative CO2 production and S. cerevisiae cell number. Moreover samples exposed to MF range from 10 Hz to 50 Hz showed the same behavior in all five replicates: lower cumulative O2 consumption, higher cumulative CO2 production and higher cell number compared to control sample. This could be important from the application aspect, in industry (food, feed, brewery etc.) and biotechnology, because changes in cells metabolism are not caused by chemical treatment.",
journal = "Chemical Engineering and Processing - Process Intensification",
title = "The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth",
volume = "143",
pages = "1-7",
doi = "10.1016/j.cep.2019.107593"
}

Bubanja, I. N., Lončarević, B. D., Lješević, M., Beškoski, V., Gojgić-Cvijović, G. D., Velikić, Z.,& Stanisavljev, D.. (2019). The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth. in Chemical Engineering and Processing - Process Intensification, 143, 1-7.
https://doi.org/10.1016/j.cep.2019.107593

Bubanja IN, Lončarević BD, Lješević M, Beškoski V, Gojgić-Cvijović GD, Velikić Z, Stanisavljev D. The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth. in Chemical Engineering and Processing - Process Intensification. 2019;143:1-7.
doi:10.1016/j.cep.2019.107593 .

Bubanja, Itana Nuša, Lončarević, Branka D., Lješević, Marija, Beškoski, Vladimir, Gojgić-Cvijović, Gordana D., Velikić, Zoran, Stanisavljev, Dragomir, "The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth" in Chemical Engineering and Processing - Process Intensification, 143 (2019):1-7,
https://doi.org/10.1016/j.cep.2019.107593 . .

TY  - JOUR
AU  - Medić, Ana
AU  - Stojanović, Ksenija A.
AU  - Izrael-Živković, Lidija
AU  - Beškoski, Vladimir
AU  - Lončarević, Branka D.
AU  - Kazazić, Saša
AU  - Karadžić, Ivanka M.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3346
AB  - The Pseudomonas aeruginosa san ai strain was investigated for its capability to degrade the 2,6-di-tert-butylphenol (2,6-DTBP) plastic additive, a hazardous and toxic substance for aquatic life. This investigation was performed under different parameter values: 2,6-DTBP concentration, inoculum size, pH, and temperature. The GC-MS study showed that P. aeruginosa efficiently degraded 2,6-DTBP in the pH range of 5-8 at higher temperatures. Under exposure to 2,6-DTBP concentrations of 2, 10, and 100 mg L-1, the strain degraded by 100, 100, and 85%, respectively, for 7 days. Crude enzyme preparation from the biomass of P. aeruginosa san ai showed higher efficiency in 2,6-DTBP removal than that shown by whole microbial cells. Gene encoding for the enzymes involved in the degradation of aromatic compounds in P. aeruginosa san ai was identified. To complement the genomic data, a comparative proteomic study of P. aeruginosa san ai grown on 2,6-DTBP or sunflower oil was conducted by means of nanoLC-MS/MS. The presence of aromatic substances resulted in the upregulation of aromatic ring cleavage enzymes, whose activity was confirmed by enzymatic tests; therefore, it could be concluded that 2,6-DTBP might be degraded by ortho-ring cleavage. A comparative proteomics study of P. aeruginosa san ai indicated that the core molecular responses to aromatic substances can be summarized as the upregulation of proteins responsible for amino acid metabolism with emphasized glutamate metabolism and energy production with upregulated enzymes of glyoxylate bypass. P. aeruginosa san ai has a high capacity to efficiently degrade aromatic compounds, and therefore its whole cells or enzymes could be used in the treatment of contaminated areas.
T2  - RSC Advances
T1  - A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-: Tert -butylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai
VL  - 9
IS  - 41
SP  - 23696
EP  - 23710
DO  - 10.1039/C9RA04298A
ER  - 

@article{
author = "Medić, Ana and Stojanović, Ksenija A. and Izrael-Živković, Lidija and Beškoski, Vladimir and Lončarević, Branka D. and Kazazić, Saša and Karadžić, Ivanka M.",
year = "2019",
abstract = "The Pseudomonas aeruginosa san ai strain was investigated for its capability to degrade the 2,6-di-tert-butylphenol (2,6-DTBP) plastic additive, a hazardous and toxic substance for aquatic life. This investigation was performed under different parameter values: 2,6-DTBP concentration, inoculum size, pH, and temperature. The GC-MS study showed that P. aeruginosa efficiently degraded 2,6-DTBP in the pH range of 5-8 at higher temperatures. Under exposure to 2,6-DTBP concentrations of 2, 10, and 100 mg L-1, the strain degraded by 100, 100, and 85%, respectively, for 7 days. Crude enzyme preparation from the biomass of P. aeruginosa san ai showed higher efficiency in 2,6-DTBP removal than that shown by whole microbial cells. Gene encoding for the enzymes involved in the degradation of aromatic compounds in P. aeruginosa san ai was identified. To complement the genomic data, a comparative proteomic study of P. aeruginosa san ai grown on 2,6-DTBP or sunflower oil was conducted by means of nanoLC-MS/MS. The presence of aromatic substances resulted in the upregulation of aromatic ring cleavage enzymes, whose activity was confirmed by enzymatic tests; therefore, it could be concluded that 2,6-DTBP might be degraded by ortho-ring cleavage. A comparative proteomics study of P. aeruginosa san ai indicated that the core molecular responses to aromatic substances can be summarized as the upregulation of proteins responsible for amino acid metabolism with emphasized glutamate metabolism and energy production with upregulated enzymes of glyoxylate bypass. P. aeruginosa san ai has a high capacity to efficiently degrade aromatic compounds, and therefore its whole cells or enzymes could be used in the treatment of contaminated areas.",
journal = "RSC Advances",
title = "A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-: Tert -butylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai",
volume = "9",
number = "41",
pages = "23696-23710",
doi = "10.1039/C9RA04298A"
}

Medić, A., Stojanović, K. A., Izrael-Živković, L., Beškoski, V., Lončarević, B. D., Kazazić, S.,& Karadžić, I. M.. (2019). A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-: Tert -butylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai. in RSC Advances, 9(41), 23696-23710.
https://doi.org/10.1039/C9RA04298A

Medić A, Stojanović KA, Izrael-Živković L, Beškoski V, Lončarević BD, Kazazić S, Karadžić IM. A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-: Tert -butylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai. in RSC Advances. 2019;9(41):23696-23710.
doi:10.1039/C9RA04298A .

Medić, Ana, Stojanović, Ksenija A., Izrael-Živković, Lidija, Beškoski, Vladimir, Lončarević, Branka D., Kazazić, Saša, Karadžić, Ivanka M., "A comprehensive study of conditions of the biodegradation of a plastic additive 2,6-di-: Tert -butylphenol and proteomic changes in the degrader Pseudomonas aeruginosa san ai" in RSC Advances, 9, no. 41 (2019):23696-23710,
https://doi.org/10.1039/C9RA04298A . .

TY  - DATA
AU  - Medić, Ana
AU  - Stojanović, Ksenija A.
AU  - Izrael-Živković, Lidija
AU  - Beškoski, Vladimir
AU  - Lončarević, Branka D.
AU  - Kazazić, Saša
AU  - Karadžić, Ivanka M.
PY  - 2019
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3347
T2  - RSC Advances
T1  - Supplementary data for the article: Medić, A.; Stojanović, K.; Izrael-Živković, L.; Beškoski, V.; Lončarević, B.; Kazazić, S.; Karadžić, I. A Comprehensive Study of Conditions of the Biodegradation of a Plastic Additive 2,6-Di-: Tert -Butylphenol and Proteomic Changes in the Degrader Pseudomonas Aeruginosa San Ai. RSC Advances 2019, 9 (41), 23696–23710. https://doi.org/10.1039/c9ra04298a
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3347
ER  - 

@misc{
author = "Medić, Ana and Stojanović, Ksenija A. and Izrael-Živković, Lidija and Beškoski, Vladimir and Lončarević, Branka D. and Kazazić, Saša and Karadžić, Ivanka M.",
year = "2019",
journal = "RSC Advances",
title = "Supplementary data for the article: Medić, A.; Stojanović, K.; Izrael-Živković, L.; Beškoski, V.; Lončarević, B.; Kazazić, S.; Karadžić, I. A Comprehensive Study of Conditions of the Biodegradation of a Plastic Additive 2,6-Di-: Tert -Butylphenol and Proteomic Changes in the Degrader Pseudomonas Aeruginosa San Ai. RSC Advances 2019, 9 (41), 23696–23710. https://doi.org/10.1039/c9ra04298a",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3347"
}

Medić, A., Stojanović, K. A., Izrael-Živković, L., Beškoski, V., Lončarević, B. D., Kazazić, S.,& Karadžić, I. M.. (2019). Supplementary data for the article: Medić, A.; Stojanović, K.; Izrael-Živković, L.; Beškoski, V.; Lončarević, B.; Kazazić, S.; Karadžić, I. A Comprehensive Study of Conditions of the Biodegradation of a Plastic Additive 2,6-Di-: Tert -Butylphenol and Proteomic Changes in the Degrader Pseudomonas Aeruginosa San Ai. RSC Advances 2019, 9 (41), 23696–23710. https://doi.org/10.1039/c9ra04298a. in RSC Advances.
https://hdl.handle.net/21.15107/rcub_cherry_3347

Medić A, Stojanović KA, Izrael-Živković L, Beškoski V, Lončarević BD, Kazazić S, Karadžić IM. Supplementary data for the article: Medić, A.; Stojanović, K.; Izrael-Živković, L.; Beškoski, V.; Lončarević, B.; Kazazić, S.; Karadžić, I. A Comprehensive Study of Conditions of the Biodegradation of a Plastic Additive 2,6-Di-: Tert -Butylphenol and Proteomic Changes in the Degrader Pseudomonas Aeruginosa San Ai. RSC Advances 2019, 9 (41), 23696–23710. https://doi.org/10.1039/c9ra04298a. in RSC Advances. 2019;.
https://hdl.handle.net/21.15107/rcub_cherry_3347 .

Medić, Ana, Stojanović, Ksenija A., Izrael-Živković, Lidija, Beškoski, Vladimir, Lončarević, Branka D., Kazazić, Saša, Karadžić, Ivanka M., "Supplementary data for the article: Medić, A.; Stojanović, K.; Izrael-Živković, L.; Beškoski, V.; Lončarević, B.; Kazazić, S.; Karadžić, I. A Comprehensive Study of Conditions of the Biodegradation of a Plastic Additive 2,6-Di-: Tert -Butylphenol and Proteomic Changes in the Degrader Pseudomonas Aeruginosa San Ai. RSC Advances 2019, 9 (41), 23696–23710. https://doi.org/10.1039/c9ra04298a" in RSC Advances (2019),
https://hdl.handle.net/21.15107/rcub_cherry_3347 .

TY  - CONF
AU  - Jakovljević, Dragica
AU  - Lončarević, Branka D.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Žerađanin, Aleksandra
AU  - Lugonja, Nikoleta
AU  - Joksimović, Kristina
AU  - Beškoski, Vladimir
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5391
AB  - In this work, pullulan based films with water extracts of medicinal herbs thyme and sage, were prepared. The resulting films were characterized by FT-IR spectroscopy and their antioxidant and antimicrobial activities were evaluated. These products have potential use in food applications, as a supplement to functional food and/or as coatings for food packaging material.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - Proceedings - Physical Chemistry 2018, 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Bioactive pullulan based edible films with medicinal herbal extracts
VL  - 2
SP  - 963
EP  - 966
UR  - https://hdl.handle.net/21.15107/rcub_cer_4958
ER  - 

@conference{
author = "Jakovljević, Dragica and Lončarević, Branka D. and Gojgić-Cvijović, Gordana D. and Žerađanin, Aleksandra and Lugonja, Nikoleta and Joksimović, Kristina and Beškoski, Vladimir",
year = "2018",
abstract = "In this work, pullulan based films with water extracts of medicinal herbs thyme and sage, were prepared. The resulting films were characterized by FT-IR spectroscopy and their antioxidant and antimicrobial activities were evaluated. These products have potential use in food applications, as a supplement to functional food and/or as coatings for food packaging material.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "Proceedings - Physical Chemistry 2018, 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Bioactive pullulan based edible films with medicinal herbal extracts",
volume = "2",
pages = "963-966",
url = "https://hdl.handle.net/21.15107/rcub_cer_4958"
}

Jakovljević, D., Lončarević, B. D., Gojgić-Cvijović, G. D., Žerađanin, A., Lugonja, N., Joksimović, K.,& Beškoski, V.. (2018). Bioactive pullulan based edible films with medicinal herbal extracts. in Proceedings - Physical Chemistry 2018, 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Belgrade : Society of Physical Chemists of Serbia., 2, 963-966.
https://hdl.handle.net/21.15107/rcub_cer_4958

Jakovljević D, Lončarević BD, Gojgić-Cvijović GD, Žerađanin A, Lugonja N, Joksimović K, Beškoski V. Bioactive pullulan based edible films with medicinal herbal extracts. in Proceedings - Physical Chemistry 2018, 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2018;2:963-966.
https://hdl.handle.net/21.15107/rcub_cer_4958 .

Jakovljević, Dragica, Lončarević, Branka D., Gojgić-Cvijović, Gordana D., Žerađanin, Aleksandra, Lugonja, Nikoleta, Joksimović, Kristina, Beškoski, Vladimir, "Bioactive pullulan based edible films with medicinal herbal extracts" in Proceedings - Physical Chemistry 2018, 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 2 (2018):963-966,
https://hdl.handle.net/21.15107/rcub_cer_4958 .

TY  - CONF
AU  - Joksimović, Kristina
AU  - Žerađanin, Aleksandra
AU  - Lješević, Marija
AU  - Lončarević, Branka D.
AU  - Jakovljević, Dragica
AU  - Gojgić-Cvijović, Gordana D.
AU  - Beškoski, Vladimir
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5403
AB  - Development of innovative granular micro-composite fertilizers based on vermicompost and metabolic activity testing are presented.
PB  - Beograd: Udruženje mikrobioologa Srbije
C3  - XII Kongres mikrobiologa Srbije sa međunadornim učešćem - Zbornik apstrakata, Beograd 10-12. maj 2018.
T1  - Inovativno granulisano organomineralno multikompozitno đubrivo na bazi vermikomposta za stimulisanje respiracije zemljišta – studija na micro-oxymax respirometru
SP  - 174
EP  - 175
UR  - https://hdl.handle.net/21.15107/rcub_cer_5031
ER  - 

@conference{
author = "Joksimović, Kristina and Žerađanin, Aleksandra and Lješević, Marija and Lončarević, Branka D. and Jakovljević, Dragica and Gojgić-Cvijović, Gordana D. and Beškoski, Vladimir",
year = "2018",
abstract = "Development of innovative granular micro-composite fertilizers based on vermicompost and metabolic activity testing are presented.",
publisher = "Beograd: Udruženje mikrobioologa Srbije",
journal = "XII Kongres mikrobiologa Srbije sa međunadornim učešćem - Zbornik apstrakata, Beograd 10-12. maj 2018.",
title = "Inovativno granulisano organomineralno multikompozitno đubrivo na bazi vermikomposta za stimulisanje respiracije zemljišta – studija na micro-oxymax respirometru",
pages = "174-175",
url = "https://hdl.handle.net/21.15107/rcub_cer_5031"
}

Joksimović, K., Žerađanin, A., Lješević, M., Lončarević, B. D., Jakovljević, D., Gojgić-Cvijović, G. D.,& Beškoski, V.. (2018). Inovativno granulisano organomineralno multikompozitno đubrivo na bazi vermikomposta za stimulisanje respiracije zemljišta – studija na micro-oxymax respirometru. in XII Kongres mikrobiologa Srbije sa međunadornim učešćem - Zbornik apstrakata, Beograd 10-12. maj 2018.
Beograd: Udruženje mikrobioologa Srbije., 174-175.
https://hdl.handle.net/21.15107/rcub_cer_5031

Joksimović K, Žerađanin A, Lješević M, Lončarević BD, Jakovljević D, Gojgić-Cvijović GD, Beškoski V. Inovativno granulisano organomineralno multikompozitno đubrivo na bazi vermikomposta za stimulisanje respiracije zemljišta – studija na micro-oxymax respirometru. in XII Kongres mikrobiologa Srbije sa međunadornim učešćem - Zbornik apstrakata, Beograd 10-12. maj 2018.. 2018;:174-175.
https://hdl.handle.net/21.15107/rcub_cer_5031 .

Joksimović, Kristina, Žerađanin, Aleksandra, Lješević, Marija, Lončarević, Branka D., Jakovljević, Dragica, Gojgić-Cvijović, Gordana D., Beškoski, Vladimir, "Inovativno granulisano organomineralno multikompozitno đubrivo na bazi vermikomposta za stimulisanje respiracije zemljišta – studija na micro-oxymax respirometru" in XII Kongres mikrobiologa Srbije sa međunadornim učešćem - Zbornik apstrakata, Beograd 10-12. maj 2018. (2018):174-175,
https://hdl.handle.net/21.15107/rcub_cer_5031 .

TY  - CONF
AU  - Joksimović, Kristina
AU  - Žerađanin, Aleksandra
AU  - Lješević, Marija
AU  - Lončarević, Branka D.
AU  - Jakovljević, Dragica
AU  - Gojgić-Cvijović, Gordana D.
AU  - Beškoski, Vladimir
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5404
AB  - Poster presented at XII Kongres mikrobiologa Srbije sa međunadornim učešćem, Beograd 10-12. maj 2018.
T1  - Inovativno granulisano organomineralno multikompozitno đubrivo na bazi vermikomposta za stimulisanje respiracije zemljišta – studija na micro-oxymax respirometru
UR  - https://hdl.handle.net/21.15107/rcub_cer_5032
ER  - 

@conference{
author = "Joksimović, Kristina and Žerađanin, Aleksandra and Lješević, Marija and Lončarević, Branka D. and Jakovljević, Dragica and Gojgić-Cvijović, Gordana D. and Beškoski, Vladimir",
year = "2018",
abstract = "Poster presented at XII Kongres mikrobiologa Srbije sa međunadornim učešćem, Beograd 10-12. maj 2018.",
title = "Inovativno granulisano organomineralno multikompozitno đubrivo na bazi vermikomposta za stimulisanje respiracije zemljišta – studija na micro-oxymax respirometru",
url = "https://hdl.handle.net/21.15107/rcub_cer_5032"
}

Joksimović, K., Žerađanin, A., Lješević, M., Lončarević, B. D., Jakovljević, D., Gojgić-Cvijović, G. D.,& Beškoski, V.. (2018). Inovativno granulisano organomineralno multikompozitno đubrivo na bazi vermikomposta za stimulisanje respiracije zemljišta – studija na micro-oxymax respirometru. .
https://hdl.handle.net/21.15107/rcub_cer_5032

Joksimović K, Žerađanin A, Lješević M, Lončarević BD, Jakovljević D, Gojgić-Cvijović GD, Beškoski V. Inovativno granulisano organomineralno multikompozitno đubrivo na bazi vermikomposta za stimulisanje respiracije zemljišta – studija na micro-oxymax respirometru. 2018;.
https://hdl.handle.net/21.15107/rcub_cer_5032 .

Joksimović, Kristina, Žerađanin, Aleksandra, Lješević, Marija, Lončarević, Branka D., Jakovljević, Dragica, Gojgić-Cvijović, Gordana D., Beškoski, Vladimir, "Inovativno granulisano organomineralno multikompozitno đubrivo na bazi vermikomposta za stimulisanje respiracije zemljišta – studija na micro-oxymax respirometru" (2018),
https://hdl.handle.net/21.15107/rcub_cer_5032 .

TY  - CONF
AU  - Lončarević, Branka D.
AU  - Nikolić, Vladimir
AU  - Lugonja, Nikoleta
AU  - Randjelović, Danijela
AU  - Gojgić-Cvijović, Gordana D.
AU  - Jakovljević, Dragica
AU  - Beškoski, Vladimir
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5426
AB  - The numerous naturally occurring biopolymeric materials are used as edible films and coatings (lipids, proteins, and polysaccharides). Microbial levan and pullulan have been already considered as biopolymers in food industry. The aim of this work was to obtain the levan and levan/pullulan blend films and compare them using the Atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR). Two different films compositions were prepared by casting method: levan and levan/pullulan in the proportions 1:1. AFM measurements showed that levan film had a smoother surface and, consequently, a lower degree of roughness comparing to the levan/pullulan blend films. FTIR spectra showed peaks, which corresponded to all components of the obtained films.
PB  - Belgrade :  Society of Physical Chemists of Serbia
C3  - Proceedings - 6th Workshop Specific Methods for Food Safety and Quality
T1  - Levan and Levan/Pullulan Blend Films: AFM and FTIR Spectroscopy Characterization
SP  - 33
EP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_cer_3983
ER  - 

@conference{
author = "Lončarević, Branka D. and Nikolić, Vladimir and Lugonja, Nikoleta and Randjelović, Danijela and Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica and Beškoski, Vladimir",
year = "2018",
abstract = "The numerous naturally occurring biopolymeric materials are used as edible films and coatings (lipids, proteins, and polysaccharides). Microbial levan and pullulan have been already considered as biopolymers in food industry. The aim of this work was to obtain the levan and levan/pullulan blend films and compare them using the Atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR). Two different films compositions were prepared by casting method: levan and levan/pullulan in the proportions 1:1. AFM measurements showed that levan film had a smoother surface and, consequently, a lower degree of roughness comparing to the levan/pullulan blend films. FTIR spectra showed peaks, which corresponded to all components of the obtained films.",
publisher = "Belgrade :  Society of Physical Chemists of Serbia",
journal = "Proceedings - 6th Workshop Specific Methods for Food Safety and Quality",
title = "Levan and Levan/Pullulan Blend Films: AFM and FTIR Spectroscopy Characterization",
pages = "33-36",
url = "https://hdl.handle.net/21.15107/rcub_cer_3983"
}

Lončarević, B. D., Nikolić, V., Lugonja, N., Randjelović, D., Gojgić-Cvijović, G. D., Jakovljević, D.,& Beškoski, V.. (2018). Levan and Levan/Pullulan Blend Films: AFM and FTIR Spectroscopy Characterization. in Proceedings - 6th Workshop Specific Methods for Food Safety and Quality
Belgrade :  Society of Physical Chemists of Serbia., 33-36.
https://hdl.handle.net/21.15107/rcub_cer_3983

Lončarević BD, Nikolić V, Lugonja N, Randjelović D, Gojgić-Cvijović GD, Jakovljević D, Beškoski V. Levan and Levan/Pullulan Blend Films: AFM and FTIR Spectroscopy Characterization. in Proceedings - 6th Workshop Specific Methods for Food Safety and Quality. 2018;:33-36.
https://hdl.handle.net/21.15107/rcub_cer_3983 .

Lončarević, Branka D., Nikolić, Vladimir, Lugonja, Nikoleta, Randjelović, Danijela, Gojgić-Cvijović, Gordana D., Jakovljević, Dragica, Beškoski, Vladimir, "Levan and Levan/Pullulan Blend Films: AFM and FTIR Spectroscopy Characterization" in Proceedings - 6th Workshop Specific Methods for Food Safety and Quality (2018):33-36,
https://hdl.handle.net/21.15107/rcub_cer_3983 .

TY  - CONF
AU  - Lončarević, Branka D.
AU  - Nikolić, Vladimir
AU  - Lugonja, Nikoleta
AU  - Randjelović, Danijela
AU  - Gojgić-Cvijović, Gordana D.
AU  - Jakovljević, Dragica
AU  - Beškoski, Vladimir
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5427
AB  - Poster presented at 6th Workshop Specific Methods for Food Safety and Quality, 2018
T1  - Levan and Levan/Pullulan Blend Films: AFM and FTIR Spectroscopy Characterization
UR  - https://hdl.handle.net/21.15107/rcub_cer_3984
ER  - 

@conference{
author = "Lončarević, Branka D. and Nikolić, Vladimir and Lugonja, Nikoleta and Randjelović, Danijela and Gojgić-Cvijović, Gordana D. and Jakovljević, Dragica and Beškoski, Vladimir",
year = "2018",
abstract = "Poster presented at 6th Workshop Specific Methods for Food Safety and Quality, 2018",
title = "Levan and Levan/Pullulan Blend Films: AFM and FTIR Spectroscopy Characterization",
url = "https://hdl.handle.net/21.15107/rcub_cer_3984"
}

Lončarević, B. D., Nikolić, V., Lugonja, N., Randjelović, D., Gojgić-Cvijović, G. D., Jakovljević, D.,& Beškoski, V.. (2018). Levan and Levan/Pullulan Blend Films: AFM and FTIR Spectroscopy Characterization. .
https://hdl.handle.net/21.15107/rcub_cer_3984

Lončarević BD, Nikolić V, Lugonja N, Randjelović D, Gojgić-Cvijović GD, Jakovljević D, Beškoski V. Levan and Levan/Pullulan Blend Films: AFM and FTIR Spectroscopy Characterization. 2018;.
https://hdl.handle.net/21.15107/rcub_cer_3984 .

Lončarević, Branka D., Nikolić, Vladimir, Lugonja, Nikoleta, Randjelović, Danijela, Gojgić-Cvijović, Gordana D., Jakovljević, Dragica, Beškoski, Vladimir, "Levan and Levan/Pullulan Blend Films: AFM and FTIR Spectroscopy Characterization" (2018),
https://hdl.handle.net/21.15107/rcub_cer_3984 .