TY  - JOUR
AU  - Nedić, Olgica
AU  - Nikolić, J. A.
AU  - Prisic, S
AU  - Aćimović, Jelena M.
AU  - Hajdukovic-Dragojlovic, L
PY  - 2003
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/543
AB  - The capacity of the liver to synthesize insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) may be compromised by alcohol. The characteristics of IGFBP-3 variants obtained from healthy individuals and patients with alcoholic cirrhosis (ALC) were compared. Concanavalin A (Con A) affinity electrophoresis and ligand blotting demonstrated that there was a gradual change in carbohydrate properties of putative IGFBP-3 with progression of ALC from stages A to C. As many as 12 ionic species of IGFBP-3 could be distinguished, corresponding probably to variously glycosylated and/or phosphorylated isoforms of the core protein. Three of them reacted significantly with the immobilized Con A, the pattern being altered in patients with ALC. Patients with ALC in stage B exhibited the presence of clearly differentiated IGFBP-3 variants less and more Con A reactive, suggesting this stage to be a turning point with the most intensive changes in the IGF-IGFBP system. Because the glycosylation pattern is tissue specific, pathological post-translational modifications found for one glycoprotein (IGFBP-3) are probably shared by others of the same tissue origin. This may affect their susceptibility to proteolysis and subsequently their function.
PB  - Carfax Publishing, Basingstoke
T2  - Addiction Biology
T1  - Reactivity of IGF binding protein-3 isoforms towards concanavalin A in healthy adults and subjects with cirrhosis
VL  - 8
IS  - 1
SP  - 81
EP  - 88
DO  - 10.1080/1355621031000069927
ER  - 

@article{
author = "Nedić, Olgica and Nikolić, J. A. and Prisic, S and Aćimović, Jelena M. and Hajdukovic-Dragojlovic, L",
year = "2003",
abstract = "The capacity of the liver to synthesize insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) may be compromised by alcohol. The characteristics of IGFBP-3 variants obtained from healthy individuals and patients with alcoholic cirrhosis (ALC) were compared. Concanavalin A (Con A) affinity electrophoresis and ligand blotting demonstrated that there was a gradual change in carbohydrate properties of putative IGFBP-3 with progression of ALC from stages A to C. As many as 12 ionic species of IGFBP-3 could be distinguished, corresponding probably to variously glycosylated and/or phosphorylated isoforms of the core protein. Three of them reacted significantly with the immobilized Con A, the pattern being altered in patients with ALC. Patients with ALC in stage B exhibited the presence of clearly differentiated IGFBP-3 variants less and more Con A reactive, suggesting this stage to be a turning point with the most intensive changes in the IGF-IGFBP system. Because the glycosylation pattern is tissue specific, pathological post-translational modifications found for one glycoprotein (IGFBP-3) are probably shared by others of the same tissue origin. This may affect their susceptibility to proteolysis and subsequently their function.",
publisher = "Carfax Publishing, Basingstoke",
journal = "Addiction Biology",
title = "Reactivity of IGF binding protein-3 isoforms towards concanavalin A in healthy adults and subjects with cirrhosis",
volume = "8",
number = "1",
pages = "81-88",
doi = "10.1080/1355621031000069927"
}

Nedić, O., Nikolić, J. A., Prisic, S., Aćimović, J. M.,& Hajdukovic-Dragojlovic, L.. (2003). Reactivity of IGF binding protein-3 isoforms towards concanavalin A in healthy adults and subjects with cirrhosis. in Addiction Biology
Carfax Publishing, Basingstoke., 8(1), 81-88.
https://doi.org/10.1080/1355621031000069927

Nedić O, Nikolić JA, Prisic S, Aćimović JM, Hajdukovic-Dragojlovic L. Reactivity of IGF binding protein-3 isoforms towards concanavalin A in healthy adults and subjects with cirrhosis. in Addiction Biology. 2003;8(1):81-88.
doi:10.1080/1355621031000069927 .

Nedić, Olgica, Nikolić, J. A., Prisic, S, Aćimović, Jelena M., Hajdukovic-Dragojlovic, L, "Reactivity of IGF binding protein-3 isoforms towards concanavalin A in healthy adults and subjects with cirrhosis" in Addiction Biology, 8, no. 1 (2003):81-88,
https://doi.org/10.1080/1355621031000069927 . .