TY  - JOUR
AU  - Ćilerdžić, Jasmina
AU  - Sofrenić, Ivana V.
AU  - Tešević, Vele
AU  - Brčeski, Ilija
AU  - Duletić-Laušević, Sonja
AU  - Vukojević, Jelena
AU  - Stajić, Mirjana
PY  - 2018
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2153
AB  - Various neurodegenerative diseases are the main challenges to the modern medicine and there is a great need for novel, natural, neuroprotective agents. Ganoderma lucidum is a well-known medicinal mushroom, which health benefits have been confirmed by numerous studies. As demand for its basidiocarps is increased and traditional cultivation on hardwoods is not environmentally friendly and economically justified, finding of alternative substrates is necessary. The aim of the study was to assess the effect of alternative cultivation substrates on the chemical profile of G.lucidum basidiocarps and their capacity to inhibit acetylcholinesterase and tyrosinase, which higher activity is directly associated with neurodegenerative processes. Extracts of basidiocarps cultivated on alternative substrates, especially on clear wheat straw, showed significantly higher inhibition capacities than extracts of commercially-grown ones. These extracts were considerably different chemically from commercial basidiocarps extracts and even nine new compounds were isolated from them. Our results suggest that cultivation substrate greatly affect the chemical profile and neuroprotective capacity of obtained basidiocarps and wheat straw is a promising cultivation substrate.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry and Biodiversity
T1  - Neuroprotective Potential and Chemical Profile of Alternatively Cultivated Ganoderma lucidum Basidiocarps
VL  - 15
IS  - 5
DO  - 10.1002/cbdv.201800036
ER  - 

@article{
author = "Ćilerdžić, Jasmina and Sofrenić, Ivana V. and Tešević, Vele and Brčeski, Ilija and Duletić-Laušević, Sonja and Vukojević, Jelena and Stajić, Mirjana",
year = "2018",
abstract = "Various neurodegenerative diseases are the main challenges to the modern medicine and there is a great need for novel, natural, neuroprotective agents. Ganoderma lucidum is a well-known medicinal mushroom, which health benefits have been confirmed by numerous studies. As demand for its basidiocarps is increased and traditional cultivation on hardwoods is not environmentally friendly and economically justified, finding of alternative substrates is necessary. The aim of the study was to assess the effect of alternative cultivation substrates on the chemical profile of G.lucidum basidiocarps and their capacity to inhibit acetylcholinesterase and tyrosinase, which higher activity is directly associated with neurodegenerative processes. Extracts of basidiocarps cultivated on alternative substrates, especially on clear wheat straw, showed significantly higher inhibition capacities than extracts of commercially-grown ones. These extracts were considerably different chemically from commercial basidiocarps extracts and even nine new compounds were isolated from them. Our results suggest that cultivation substrate greatly affect the chemical profile and neuroprotective capacity of obtained basidiocarps and wheat straw is a promising cultivation substrate.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry and Biodiversity",
title = "Neuroprotective Potential and Chemical Profile of Alternatively Cultivated Ganoderma lucidum Basidiocarps",
volume = "15",
number = "5",
doi = "10.1002/cbdv.201800036"
}

Ćilerdžić, J., Sofrenić, I. V., Tešević, V., Brčeski, I., Duletić-Laušević, S., Vukojević, J.,& Stajić, M.. (2018). Neuroprotective Potential and Chemical Profile of Alternatively Cultivated Ganoderma lucidum Basidiocarps. in Chemistry and Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 15(5).
https://doi.org/10.1002/cbdv.201800036

Ćilerdžić J, Sofrenić IV, Tešević V, Brčeski I, Duletić-Laušević S, Vukojević J, Stajić M. Neuroprotective Potential and Chemical Profile of Alternatively Cultivated Ganoderma lucidum Basidiocarps. in Chemistry and Biodiversity. 2018;15(5).
doi:10.1002/cbdv.201800036 .

Ćilerdžić, Jasmina, Sofrenić, Ivana V., Tešević, Vele, Brčeski, Ilija, Duletić-Laušević, Sonja, Vukojević, Jelena, Stajić, Mirjana, "Neuroprotective Potential and Chemical Profile of Alternatively Cultivated Ganoderma lucidum Basidiocarps" in Chemistry and Biodiversity, 15, no. 5 (2018),
https://doi.org/10.1002/cbdv.201800036 . .

TY  - JOUR
AU  - Simonic, Jasmina
AU  - Stajić, Mirjana
AU  - Glamočlija, Jasmina
AU  - Vukojević, Jelena
AU  - Duletić-Laušević, Sonja
AU  - Brčeski, Ilija
PY  - 2008
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/979
AB  - This study showed that cultivation conditions (medium composition, initial medium pH, aeration rate, inoculum density) affect biomass and polysaccharide production in Ganoderma lucidum strain HAI 447. The synthetic medium (pH 5.5) with maltose at the concentration of 65.0 g L-1, peptone of 0.2%, as well as in the presence of K, Na, and Mg at concentrations of 6.0, 3.0, and 2.0 mM, respectively, presented the best conditions for biomass (29.2 g L-1) and extra- and intracellular polysaccharide (2.9 mg mL(-1) and 73.3 mg g(-1), respectively) production in the analyzed strain. Constant aeration was optimal for biomass and extracellular polysaccharide production, whereas cultivation under stationary conditions was the best for intracellular polysaccharide production. A peak in biomass and polysaccharide production was observed at an inoculum density of 20.0 mL.
PB  - Begell House Inc, Redding
T2  - International Journal of Medicinal Mushrooms
T1  - Optimization of Submerged Cultivation Conditions for Extra- and Intracellular Polysaccharide Production by Medicinal Ling Zhi or Reishi Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae)
VL  - 10
IS  - 4
SP  - 351
EP  - 360
DO  - 10.1615/IntJMedMushr.v10.i4.80
ER  - 

@article{
author = "Simonic, Jasmina and Stajić, Mirjana and Glamočlija, Jasmina and Vukojević, Jelena and Duletić-Laušević, Sonja and Brčeski, Ilija",
year = "2008",
abstract = "This study showed that cultivation conditions (medium composition, initial medium pH, aeration rate, inoculum density) affect biomass and polysaccharide production in Ganoderma lucidum strain HAI 447. The synthetic medium (pH 5.5) with maltose at the concentration of 65.0 g L-1, peptone of 0.2%, as well as in the presence of K, Na, and Mg at concentrations of 6.0, 3.0, and 2.0 mM, respectively, presented the best conditions for biomass (29.2 g L-1) and extra- and intracellular polysaccharide (2.9 mg mL(-1) and 73.3 mg g(-1), respectively) production in the analyzed strain. Constant aeration was optimal for biomass and extracellular polysaccharide production, whereas cultivation under stationary conditions was the best for intracellular polysaccharide production. A peak in biomass and polysaccharide production was observed at an inoculum density of 20.0 mL.",
publisher = "Begell House Inc, Redding",
journal = "International Journal of Medicinal Mushrooms",
title = "Optimization of Submerged Cultivation Conditions for Extra- and Intracellular Polysaccharide Production by Medicinal Ling Zhi or Reishi Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae)",
volume = "10",
number = "4",
pages = "351-360",
doi = "10.1615/IntJMedMushr.v10.i4.80"
}

Simonic, J., Stajić, M., Glamočlija, J., Vukojević, J., Duletić-Laušević, S.,& Brčeski, I.. (2008). Optimization of Submerged Cultivation Conditions for Extra- and Intracellular Polysaccharide Production by Medicinal Ling Zhi or Reishi Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae). in International Journal of Medicinal Mushrooms
Begell House Inc, Redding., 10(4), 351-360.
https://doi.org/10.1615/IntJMedMushr.v10.i4.80

Simonic J, Stajić M, Glamočlija J, Vukojević J, Duletić-Laušević S, Brčeski I. Optimization of Submerged Cultivation Conditions for Extra- and Intracellular Polysaccharide Production by Medicinal Ling Zhi or Reishi Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae). in International Journal of Medicinal Mushrooms. 2008;10(4):351-360.
doi:10.1615/IntJMedMushr.v10.i4.80 .

Simonic, Jasmina, Stajić, Mirjana, Glamočlija, Jasmina, Vukojević, Jelena, Duletić-Laušević, Sonja, Brčeski, Ilija, "Optimization of Submerged Cultivation Conditions for Extra- and Intracellular Polysaccharide Production by Medicinal Ling Zhi or Reishi Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae)" in International Journal of Medicinal Mushrooms, 10, no. 4 (2008):351-360,
https://doi.org/10.1615/IntJMedMushr.v10.i4.80 . .

TY  - JOUR
AU  - Janaćković, Peđa T.
AU  - Tešević, Vele
AU  - Marin, Petar D.
AU  - Milosljevic, S.
AU  - Duletić-Laušević, Sonja
AU  - Janackovic, Slavica
AU  - Veljić, Milan
PY  - 2008
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/597
AB  - Ether extracts of 15 Centaurea L. species (Asteraceae) methanol extracts of 12 species, and cnicin isolated from C derventana were tested for general bioactivity using the brine shrimp lethality test. Cnicin showed the most potent activity with LC50 0.2. Also, ether extract of C. splendens showed significant activity with LC50 7.3, as did methanol extract of C. arenaria with LC50 12.4.
AB  - U radu je ispitivana biološka aktivnost metanolnih i etarskih ekstrakata 18 vrsta roda Centaurea pomoću Biotesta Artemiasalina. Vrste ovog roda predstavljaju značajne prirodne izvore biološki aktivnih sekundarnih metabolita. Takođe, određena je i biološka aktivnost (toksičnost) seskviterpenskog laktona knicina, čiste supstance, izdvojene iz C. derventana. Ekstrakti su dobijeni ekstrakcijom 5 g listova u etru i metanolu, knicin je izolovan iz C derventana, prema Tešević et. al. (1998). Uzorci i test su pripremljeni i sprovedeni prema 2Meyeret al. (1982) i McLaughlin (-1991). Kao kontrola korišćen je kalijum dihromat (VI). Utvrđeno je da metanolni i etarski ekstrakti svih ispitivanih vrsta pokazuju značajnu biološku aktivnost. Najveću aktivnost ispitivanih metanolnih ekstrakata pokazao je ekstrakt vrste C. arenaria LC50 12.4 μg/ml, dok je najmanju aktivnost pokazala vrsta C. chrysolepis, čija vrednost LC50 iznosi 277.7 μg/ml. Najveću aktivnost etarskih ekstrakata pokazao je ekstrakt vrste C. splendens, čija vrednost LC50 iznosi 7.3 μg/ml, a najmanju aktivnost pokazala je vrsta C. scabiosa čija vrednost LC50 iznosi 2605.9 μg/ml, tj. - gt 1000. Seskviterpenski lakton knicin je pokazao najveću biološku aktivnost (LC50 0.2μg/ml). Rezultati biotesta soni račić (Artemia salina Leach) pokazuju značajnu aktivnost svih ispitivanih ekstrakata analiziranih vrsta roda Centaurea. Naši rezultati, literaturni podaci, kao i upotreba vrsta roda Centaurea u etnomedicini, navode na zaključak da je istraživanje vrsta ovog roda sa aspekta biološke aktivnosti kako ukupnog ekstrakta, tako i pojedinačnih komponenti, veoma značajno. Pored ostalog, na osnovu rezultata može se zaključiti da biotest soni račić predstavlja jednostavan, statistički prihvatljiv, brz i pouzdan metod za detekciju komponenti viših biljaka koje mogu ispoljavati čitav spektar farmakoloških aktivnosti.
PB  - Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd
T2  - Archives of biological sciences
T1  - Brine Shrimp Lethality Bioassay of Selected Centaurea L. Species (Asteraceae)
T1  - Biotest artemia salina kod odabranih vrsta roda Centaurea L. (Asteraceae)
VL  - 60
IS  - 4
SP  - 681
EP  - 685
DO  - 10.2298/ABS0804681J
ER  - 

@article{
author = "Janaćković, Peđa T. and Tešević, Vele and Marin, Petar D. and Milosljevic, S. and Duletić-Laušević, Sonja and Janackovic, Slavica and Veljić, Milan",
year = "2008",
abstract = "Ether extracts of 15 Centaurea L. species (Asteraceae) methanol extracts of 12 species, and cnicin isolated from C derventana were tested for general bioactivity using the brine shrimp lethality test. Cnicin showed the most potent activity with LC50 0.2. Also, ether extract of C. splendens showed significant activity with LC50 7.3, as did methanol extract of C. arenaria with LC50 12.4., U radu je ispitivana biološka aktivnost metanolnih i etarskih ekstrakata 18 vrsta roda Centaurea pomoću Biotesta Artemiasalina. Vrste ovog roda predstavljaju značajne prirodne izvore biološki aktivnih sekundarnih metabolita. Takođe, određena je i biološka aktivnost (toksičnost) seskviterpenskog laktona knicina, čiste supstance, izdvojene iz C. derventana. Ekstrakti su dobijeni ekstrakcijom 5 g listova u etru i metanolu, knicin je izolovan iz C derventana, prema Tešević et. al. (1998). Uzorci i test su pripremljeni i sprovedeni prema 2Meyeret al. (1982) i McLaughlin (-1991). Kao kontrola korišćen je kalijum dihromat (VI). Utvrđeno je da metanolni i etarski ekstrakti svih ispitivanih vrsta pokazuju značajnu biološku aktivnost. Najveću aktivnost ispitivanih metanolnih ekstrakata pokazao je ekstrakt vrste C. arenaria LC50 12.4 μg/ml, dok je najmanju aktivnost pokazala vrsta C. chrysolepis, čija vrednost LC50 iznosi 277.7 μg/ml. Najveću aktivnost etarskih ekstrakata pokazao je ekstrakt vrste C. splendens, čija vrednost LC50 iznosi 7.3 μg/ml, a najmanju aktivnost pokazala je vrsta C. scabiosa čija vrednost LC50 iznosi 2605.9 μg/ml, tj. - gt 1000. Seskviterpenski lakton knicin je pokazao najveću biološku aktivnost (LC50 0.2μg/ml). Rezultati biotesta soni račić (Artemia salina Leach) pokazuju značajnu aktivnost svih ispitivanih ekstrakata analiziranih vrsta roda Centaurea. Naši rezultati, literaturni podaci, kao i upotreba vrsta roda Centaurea u etnomedicini, navode na zaključak da je istraživanje vrsta ovog roda sa aspekta biološke aktivnosti kako ukupnog ekstrakta, tako i pojedinačnih komponenti, veoma značajno. Pored ostalog, na osnovu rezultata može se zaključiti da biotest soni račić predstavlja jednostavan, statistički prihvatljiv, brz i pouzdan metod za detekciju komponenti viših biljaka koje mogu ispoljavati čitav spektar farmakoloških aktivnosti.",
publisher = "Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd",
journal = "Archives of biological sciences",
title = "Brine Shrimp Lethality Bioassay of Selected Centaurea L. Species (Asteraceae), Biotest artemia salina kod odabranih vrsta roda Centaurea L. (Asteraceae)",
volume = "60",
number = "4",
pages = "681-685",
doi = "10.2298/ABS0804681J"
}

Janaćković, P. T., Tešević, V., Marin, P. D., Milosljevic, S., Duletić-Laušević, S., Janackovic, S.,& Veljić, M.. (2008). Brine Shrimp Lethality Bioassay of Selected Centaurea L. Species (Asteraceae). in Archives of biological sciences
Inst Bioloska Istrazivanja Sinisa Stankovic, Beograd., 60(4), 681-685.
https://doi.org/10.2298/ABS0804681J

Janaćković PT, Tešević V, Marin PD, Milosljevic S, Duletić-Laušević S, Janackovic S, Veljić M. Brine Shrimp Lethality Bioassay of Selected Centaurea L. Species (Asteraceae). in Archives of biological sciences. 2008;60(4):681-685.
doi:10.2298/ABS0804681J .

Janaćković, Peđa T., Tešević, Vele, Marin, Petar D., Milosljevic, S., Duletić-Laušević, Sonja, Janackovic, Slavica, Veljić, Milan, "Brine Shrimp Lethality Bioassay of Selected Centaurea L. Species (Asteraceae)" in Archives of biological sciences, 60, no. 4 (2008):681-685,
https://doi.org/10.2298/ABS0804681J . .