Gopčević, Kristina

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  • Gopčević, Kristina (7)
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Author's Bibliography

Redox Status and Antioxidative Cofactor Metals Influence Clinical and Pathological Characteristics of Papillary Thyroid Carcinoma and Colloid Goiter

Rovčanin, Branislav; Stojsavljević, Aleksandar; Kekić, Dušan; Gopčević, Kristina; Manojlović, Dragan D.; Jovanović, Milan; Knežević, Saša; Živaljević, Vladan; Diklić, Aleksandar; Paunovic, Ivan

(2020)

TY  - JOUR
AU  - Rovčanin, Branislav
AU  - Stojsavljević, Aleksandar
AU  - Kekić, Dušan
AU  - Gopčević, Kristina
AU  - Manojlović, Dragan D.
AU  - Jovanović, Milan
AU  - Knežević, Saša
AU  - Živaljević, Vladan
AU  - Diklić, Aleksandar
AU  - Paunovic, Ivan
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4282
AB  - Papillary thyroid carcinoma (PTC) is the endocrine neoplasm that occurs the most often worldwide, and its molecular pathophysiology is still not well characterized. Redox status is recognized as an important factor of carcinogenesis, but its influence on the PTC's clinical course needs to be better elucidated. The aim of this research was to determine the tissue redox status of 65 PTC and 45 colloid goiter (CG) patients together with antioxidative cofactor metal profiling. The malondialdehyde (MDA) concentration was used to access the prooxidation level, while antioxidant mechanisms were estimated by assaying the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR). The antioxidative cofactor metals included quantification of Se, Cu, Zn, and Mn concentration. PTC tissues had normal prooxidation levels and increased GPx and GR activity. The activity of SOD has been significantly reduced in multicentric PTC dissemination and increased in smokers. SOD activity was directly dependent on MDA levels in CG tissues. CG patients with retrosternal goiter had reduced MDA concentration and SOD activity. Numerous correlations between redox parameters in PTC tissues reveal good co-activation of antioxidative mechanisms and cooperative response on prooxidation. PTC tissues had decreased Se levels and increased concentration of Cu and Mn in comparison to other tissues. MDA concentration and SOD activity were significant predictors of PTC's multicentric dissemination and for the existence of lymph node metastases, respectively. Particularly, the concentration of Cu predicted the retrosternal localization in CG patients. Significant findings presented in this study provide a possibility for development of novel prognostic molecular biomarkers of PTC and CG.
T2  - Biological Trace Element Research
T1  - Redox Status and Antioxidative Cofactor Metals Influence Clinical and Pathological Characteristics of Papillary Thyroid Carcinoma and Colloid Goiter
VL  - 197
IS  - 2
SP  - 349
EP  - 359
DO  - 10.1007/s12011-019-01995-x
ER  - 
@article{
author = "Rovčanin, Branislav and Stojsavljević, Aleksandar and Kekić, Dušan and Gopčević, Kristina and Manojlović, Dragan D. and Jovanović, Milan and Knežević, Saša and Živaljević, Vladan and Diklić, Aleksandar and Paunovic, Ivan",
year = "2020",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/4282",
abstract = "Papillary thyroid carcinoma (PTC) is the endocrine neoplasm that occurs the most often worldwide, and its molecular pathophysiology is still not well characterized. Redox status is recognized as an important factor of carcinogenesis, but its influence on the PTC's clinical course needs to be better elucidated. The aim of this research was to determine the tissue redox status of 65 PTC and 45 colloid goiter (CG) patients together with antioxidative cofactor metal profiling. The malondialdehyde (MDA) concentration was used to access the prooxidation level, while antioxidant mechanisms were estimated by assaying the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR). The antioxidative cofactor metals included quantification of Se, Cu, Zn, and Mn concentration. PTC tissues had normal prooxidation levels and increased GPx and GR activity. The activity of SOD has been significantly reduced in multicentric PTC dissemination and increased in smokers. SOD activity was directly dependent on MDA levels in CG tissues. CG patients with retrosternal goiter had reduced MDA concentration and SOD activity. Numerous correlations between redox parameters in PTC tissues reveal good co-activation of antioxidative mechanisms and cooperative response on prooxidation. PTC tissues had decreased Se levels and increased concentration of Cu and Mn in comparison to other tissues. MDA concentration and SOD activity were significant predictors of PTC's multicentric dissemination and for the existence of lymph node metastases, respectively. Particularly, the concentration of Cu predicted the retrosternal localization in CG patients. Significant findings presented in this study provide a possibility for development of novel prognostic molecular biomarkers of PTC and CG.",
journal = "Biological Trace Element Research",
title = "Redox Status and Antioxidative Cofactor Metals Influence Clinical and Pathological Characteristics of Papillary Thyroid Carcinoma and Colloid Goiter",
volume = "197",
number = "2",
pages = "349-359",
doi = "10.1007/s12011-019-01995-x"
}
Rovčanin, B., Stojsavljević, A., Kekić, D., Gopčević, K., Manojlović, D. D., Jovanović, M., Knežević, S., Živaljević, V., Diklić, A.,& Paunovic, I. (2020). Redox Status and Antioxidative Cofactor Metals Influence Clinical and Pathological Characteristics of Papillary Thyroid Carcinoma and Colloid Goiter.
Biological Trace Element Research, 197(2), 349-359.
https://doi.org/10.1007/s12011-019-01995-x
Rovčanin B, Stojsavljević A, Kekić D, Gopčević K, Manojlović DD, Jovanović M, Knežević S, Živaljević V, Diklić A, Paunovic I. Redox Status and Antioxidative Cofactor Metals Influence Clinical and Pathological Characteristics of Papillary Thyroid Carcinoma and Colloid Goiter. Biological Trace Element Research. 2020;197(2):349-359
Rovčanin Branislav, Stojsavljević Aleksandar, Kekić Dušan, Gopčević Kristina, Manojlović Dragan D., Jovanović Milan, Knežević Saša, Živaljević Vladan, Diklić Aleksandar, Paunovic Ivan, "Redox Status and Antioxidative Cofactor Metals Influence Clinical and Pathological Characteristics of Papillary Thyroid Carcinoma and Colloid Goiter" Biological Trace Element Research, 197, no. 2 (2020):349-359,
https://doi.org/10.1007/s12011-019-01995-x .
1
1
1

Correction to: Redox Status and Antioxidative Cofactor Metals Influence Clinical and Pathological Characteristics of Papillary Thyroid Carcinoma and Colloid Goiter (Biological Trace Element Research, (2020), 197, 2, (349-359), 10.1007/s12011-019-01995-x)

Rovčanin, Branislav; Stojsavljević, Aleksandar; Kekić, Dušan; Gopčević, Kristina; Manojlović, Dragan D.; Jovanović, Milan; Knežević, Saša; Živaljević, Vladan; Diklić, Aleksandar; Paunovic, Ivan

(Springer, 2020)

TY  - JOUR
AU  - Rovčanin, Branislav
AU  - Stojsavljević, Aleksandar
AU  - Kekić, Dušan
AU  - Gopčević, Kristina
AU  - Manojlović, Dragan D.
AU  - Jovanović, Milan
AU  - Knežević, Saša
AU  - Živaljević, Vladan
AU  - Diklić, Aleksandar
AU  - Paunovic, Ivan
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4283
AB  - Papillary thyroid carcinoma (PTC) is the endocrine neoplasm that occurs the most often worldwide, and its molecular pathophysiology is still not well characterized. Redox status is recognized as an important factor of carcinogenesis, but its influence on the PTC's clinical course needs to be better elucidated. The aim of this research was to determine the tissue redox status of 65 PTC and 45 colloid goiter (CG) patients together with antioxidative cofactor metal profiling. The malondialdehyde (MDA) concentration was used to access the prooxidation level, while antioxidant mechanisms were estimated by assaying the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR). The antioxidative cofactor metals included quantification of Se, Cu, Zn, and Mn concentration. PTC tissues had normal prooxidation levels and increased GPx and GR activity. The activity of SOD has been significantly reduced in multicentric PTC dissemination and increased in smokers. SOD activity was directly dependent on MDA levels in CG tissues. CG patients with retrosternal goiter had reduced MDA concentration and SOD activity. Numerous correlations between redox parameters in PTC tissues reveal good co-activation of antioxidative mechanisms and cooperative response on prooxidation. PTC tissues had decreased Se levels and increased concentration of Cu and Mn in comparison to other tissues. MDA concentration and SOD activity were significant predictors of PTC's multicentric dissemination and for the existence of lymph node metastases, respectively. Particularly, the concentration of Cu predicted the retrosternal localization in CG patients. Significant findings presented in this study provide a possibility for development of novel prognostic molecular biomarkers of PTC and CG.
PB  - Springer
T2  - Biological Trace Element Research
T1  - Correction to: Redox Status and Antioxidative Cofactor Metals Influence Clinical and Pathological Characteristics of Papillary Thyroid Carcinoma and Colloid Goiter (Biological Trace Element Research, (2020), 197, 2, (349-359), 10.1007/s12011-019-01995-x)
VL  - 197
IS  - 2
SP  - 349
EP  - 359
DO  - 10.1007/s12011-019-01995-x
ER  - 
@article{
author = "Rovčanin, Branislav and Stojsavljević, Aleksandar and Kekić, Dušan and Gopčević, Kristina and Manojlović, Dragan D. and Jovanović, Milan and Knežević, Saša and Živaljević, Vladan and Diklić, Aleksandar and Paunovic, Ivan",
year = "2020",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/4283",
abstract = "Papillary thyroid carcinoma (PTC) is the endocrine neoplasm that occurs the most often worldwide, and its molecular pathophysiology is still not well characterized. Redox status is recognized as an important factor of carcinogenesis, but its influence on the PTC's clinical course needs to be better elucidated. The aim of this research was to determine the tissue redox status of 65 PTC and 45 colloid goiter (CG) patients together with antioxidative cofactor metal profiling. The malondialdehyde (MDA) concentration was used to access the prooxidation level, while antioxidant mechanisms were estimated by assaying the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR). The antioxidative cofactor metals included quantification of Se, Cu, Zn, and Mn concentration. PTC tissues had normal prooxidation levels and increased GPx and GR activity. The activity of SOD has been significantly reduced in multicentric PTC dissemination and increased in smokers. SOD activity was directly dependent on MDA levels in CG tissues. CG patients with retrosternal goiter had reduced MDA concentration and SOD activity. Numerous correlations between redox parameters in PTC tissues reveal good co-activation of antioxidative mechanisms and cooperative response on prooxidation. PTC tissues had decreased Se levels and increased concentration of Cu and Mn in comparison to other tissues. MDA concentration and SOD activity were significant predictors of PTC's multicentric dissemination and for the existence of lymph node metastases, respectively. Particularly, the concentration of Cu predicted the retrosternal localization in CG patients. Significant findings presented in this study provide a possibility for development of novel prognostic molecular biomarkers of PTC and CG.",
publisher = "Springer",
journal = "Biological Trace Element Research",
title = "Correction to: Redox Status and Antioxidative Cofactor Metals Influence Clinical and Pathological Characteristics of Papillary Thyroid Carcinoma and Colloid Goiter (Biological Trace Element Research, (2020), 197, 2, (349-359), 10.1007/s12011-019-01995-x)",
volume = "197",
number = "2",
pages = "349-359",
doi = "10.1007/s12011-019-01995-x"
}
Rovčanin, B., Stojsavljević, A., Kekić, D., Gopčević, K., Manojlović, D. D., Jovanović, M., Knežević, S., Živaljević, V., Diklić, A.,& Paunovic, I. (2020). Correction to: Redox Status and Antioxidative Cofactor Metals Influence Clinical and Pathological Characteristics of Papillary Thyroid Carcinoma and Colloid Goiter (Biological Trace Element Research, (2020), 197, 2, (349-359), 10.1007/s12011-019-01995-x).
Biological Trace Element Research
Springer., 197(2), 349-359.
https://doi.org/10.1007/s12011-019-01995-x
Rovčanin B, Stojsavljević A, Kekić D, Gopčević K, Manojlović DD, Jovanović M, Knežević S, Živaljević V, Diklić A, Paunovic I. Correction to: Redox Status and Antioxidative Cofactor Metals Influence Clinical and Pathological Characteristics of Papillary Thyroid Carcinoma and Colloid Goiter (Biological Trace Element Research, (2020), 197, 2, (349-359), 10.1007/s12011-019-01995-x). Biological Trace Element Research. 2020;197(2):349-359
Rovčanin Branislav, Stojsavljević Aleksandar, Kekić Dušan, Gopčević Kristina, Manojlović Dragan D., Jovanović Milan, Knežević Saša, Živaljević Vladan, Diklić Aleksandar, Paunovic Ivan, "Correction to: Redox Status and Antioxidative Cofactor Metals Influence Clinical and Pathological Characteristics of Papillary Thyroid Carcinoma and Colloid Goiter (Biological Trace Element Research, (2020), 197, 2, (349-359), 10.1007/s12011-019-01995-x)" Biological Trace Element Research, 197, no. 2 (2020):349-359,
https://doi.org/10.1007/s12011-019-01995-x .
1
1

Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai

Izrael-Živković, Lidija; Rikalović, Milena G.; Gojgić-Cvijović, Gordana D.; Kazazić, Saša; Vrvić, Miroslav M.; Brčeski, Ilija; Beškoski, Vladimir; Lončarević, Branka D.; Gopčević, Kristina; Karadžić, Ivanka M.

(Royal Soc Chemistry, Cambridge, 2018)

TY  - JOUR
AU  - Izrael-Živković, Lidija
AU  - Rikalović, Milena G.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Kazazić, Saša
AU  - Vrvić, Miroslav M.
AU  - Brčeski, Ilija
AU  - Beškoski, Vladimir
AU  - Lončarević, Branka D.
AU  - Gopčević, Kristina
AU  - Karadžić, Ivanka M.
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2118
AB  - Pseudomonas aeruginosa san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of P. aeruginosa san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of cadmium on protein level by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins. Size exclusion chromatography was used for protein prefractionation to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 60 proteins were observed as up-regulated in cadmium-amended culture. Almost a third of the total numbers of up-regulated were metalloproteins. Particularly interesting are denitrification proteins which are over expressed but not active, suggesting their protective role in conditions of heavy metal exposure. P. aeruginosa san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in: cell energy metabolism, including denitrification proteins; amino acid metabolism; cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium-amended culture versus control was found. Our results signify that P. aeruginosa san ai is naturally well equipped to overcome and survive high doses of cadmium and, as such, has a great potential for application in bioremediation of cadmium polluted sites.
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai
VL  - 8
IS  - 19
SP  - 10549
EP  - 10560
DO  - 10.1039/c8ra00371h
ER  - 
@article{
author = "Izrael-Živković, Lidija and Rikalović, Milena G. and Gojgić-Cvijović, Gordana D. and Kazazić, Saša and Vrvić, Miroslav M. and Brčeski, Ilija and Beškoski, Vladimir and Lončarević, Branka D. and Gopčević, Kristina and Karadžić, Ivanka M.",
year = "2018",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2118",
abstract = "Pseudomonas aeruginosa san ai is a promising candidate for bioremediation of cadmium pollution, as it resists a high concentration of up to 7.2 mM of cadmium. Leaving biomass of P. aeruginosa san ai exposed to cadmium has a large biosorption potential, implying its capacity to extract heavy metal from contaminated medium. In the present study, we investigated tolerance and accumulation of cadmium on protein level by shotgun proteomics approach based on liquid chromatography and tandem mass spectrometry coupled with bioinformatics to identify proteins. Size exclusion chromatography was used for protein prefractionation to preserve native forms of metalloproteins and protein complexes. Using this approach a total of 60 proteins were observed as up-regulated in cadmium-amended culture. Almost a third of the total numbers of up-regulated were metalloproteins. Particularly interesting are denitrification proteins which are over expressed but not active, suggesting their protective role in conditions of heavy metal exposure. P. aeruginosa san ai developed a complex mechanism to adapt to cadmium, based on: extracellular biosorption, bioaccumulation, the formation of biofilm, controlled siderophore production, enhanced respiration and modified protein profile. An increased abundance of proteins involved in: cell energy metabolism, including denitrification proteins; amino acid metabolism; cell motility and posttranslational modifications, primarily based on thiol-disulfide exchange, were observed. Enhanced oxygen consumption of biomass in cadmium-amended culture versus control was found. Our results signify that P. aeruginosa san ai is naturally well equipped to overcome and survive high doses of cadmium and, as such, has a great potential for application in bioremediation of cadmium polluted sites.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai",
volume = "8",
number = "19",
pages = "10549-10560",
doi = "10.1039/c8ra00371h"
}
Izrael-Živković, L., Rikalović, M. G., Gojgić-Cvijović, G. D., Kazazić, S., Vrvić, M. M., Brčeski, I., Beškoski, V., Lončarević, B. D., Gopčević, K.,& Karadžić, I. M. (2018). Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai.
RSC Advances
Royal Soc Chemistry, Cambridge., 8(19), 10549-10560.
https://doi.org/10.1039/c8ra00371h
Izrael-Živković L, Rikalović MG, Gojgić-Cvijović GD, Kazazić S, Vrvić MM, Brčeski I, Beškoski V, Lončarević BD, Gopčević K, Karadžić IM. Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai. RSC Advances. 2018;8(19):10549-10560
Izrael-Živković Lidija, Rikalović Milena G., Gojgić-Cvijović Gordana D., Kazazić Saša, Vrvić Miroslav M., Brčeski Ilija, Beškoski Vladimir, Lončarević Branka D., Gopčević Kristina, Karadžić Ivanka M., "Cadmium specific proteomic responses of a highly resistant Pseudomonas aeruginosa san ai" RSC Advances, 8, no. 19 (2018):10549-10560,
https://doi.org/10.1039/c8ra00371h .
1
27
17
21

Supplementary material for the article: Izrael-Živković, L.; Rikalović, M.; Gojgić-Cvijović, G.; Kazazić, S.; Vrvić, M.; Brčeski, I.; Beškoski, V.; Lončarević, B.; Gopčević, K.; Karadžić, I. Cadmium Specific Proteomic Responses of a Highly Resistant: Pseudomonas Aeruginosa San Ai. RSC Advances 2018, 8 (19), 10549–10560. https://doi.org/10.1039/c8ra00371h

Izrael-Živković, Lidija; Rikalović, Milena G.; Gojgić-Cvijović, Gordana D.; Kazazić, Saša; Vrvić, Miroslav M.; Brčeski, Ilija; Beškoski, Vladimir; Lončarević, Branka D.; Gopčević, Kristina; Karadžić, Ivanka M.

(Royal Soc Chemistry, Cambridge, 2018)

TY  - BOOK
AU  - Izrael-Živković, Lidija
AU  - Rikalović, Milena G.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Kazazić, Saša
AU  - Vrvić, Miroslav M.
AU  - Brčeski, Ilija
AU  - Beškoski, Vladimir
AU  - Lončarević, Branka D.
AU  - Gopčević, Kristina
AU  - Karadžić, Ivanka M.
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3179
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - Supplementary material for the article: Izrael-Živković, L.; Rikalović, M.; Gojgić-Cvijović, G.; Kazazić, S.; Vrvić, M.; Brčeski, I.;  Beškoski, V.; Lončarević, B.; Gopčević, K.; Karadžić, I. Cadmium Specific Proteomic  Responses of a Highly Resistant: Pseudomonas Aeruginosa San Ai. RSC Advances 2018, 8  (19), 10549–10560. https://doi.org/10.1039/c8ra00371h
ER  - 
@book{
author = "Izrael-Živković, Lidija and Rikalović, Milena G. and Gojgić-Cvijović, Gordana D. and Kazazić, Saša and Vrvić, Miroslav M. and Brčeski, Ilija and Beškoski, Vladimir and Lončarević, Branka D. and Gopčević, Kristina and Karadžić, Ivanka M.",
year = "2018",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3179",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "Supplementary material for the article: Izrael-Živković, L.; Rikalović, M.; Gojgić-Cvijović, G.; Kazazić, S.; Vrvić, M.; Brčeski, I.;  Beškoski, V.; Lončarević, B.; Gopčević, K.; Karadžić, I. Cadmium Specific Proteomic  Responses of a Highly Resistant: Pseudomonas Aeruginosa San Ai. RSC Advances 2018, 8  (19), 10549–10560. https://doi.org/10.1039/c8ra00371h"
}
Izrael-Živković, L., Rikalović, M. G., Gojgić-Cvijović, G. D., Kazazić, S., Vrvić, M. M., Brčeski, I., Beškoski, V., Lončarević, B. D., Gopčević, K.,& Karadžić, I. M. (2018). Supplementary material for the article: Izrael-Živković, L.; Rikalović, M.; Gojgić-Cvijović, G.; Kazazić, S.; Vrvić, M.; Brčeski, I.;  Beškoski, V.; Lončarević, B.; Gopčević, K.; Karadžić, I. Cadmium Specific Proteomic  Responses of a Highly Resistant: Pseudomonas Aeruginosa San Ai. RSC Advances 2018, 8  (19), 10549–10560. https://doi.org/10.1039/c8ra00371h.
RSC Advances
Royal Soc Chemistry, Cambridge..
Izrael-Živković L, Rikalović MG, Gojgić-Cvijović GD, Kazazić S, Vrvić MM, Brčeski I, Beškoski V, Lončarević BD, Gopčević K, Karadžić IM. Supplementary material for the article: Izrael-Živković, L.; Rikalović, M.; Gojgić-Cvijović, G.; Kazazić, S.; Vrvić, M.; Brčeski, I.;  Beškoski, V.; Lončarević, B.; Gopčević, K.; Karadžić, I. Cadmium Specific Proteomic  Responses of a Highly Resistant: Pseudomonas Aeruginosa San Ai. RSC Advances 2018, 8  (19), 10549–10560. https://doi.org/10.1039/c8ra00371h. RSC Advances. 2018;
Izrael-Živković Lidija, Rikalović Milena G., Gojgić-Cvijović Gordana D., Kazazić Saša, Vrvić Miroslav M., Brčeski Ilija, Beškoski Vladimir, Lončarević Branka D., Gopčević Kristina, Karadžić Ivanka M., "Supplementary material for the article: Izrael-Živković, L.; Rikalović, M.; Gojgić-Cvijović, G.; Kazazić, S.; Vrvić, M.; Brčeski, I.;  Beškoski, V.; Lončarević, B.; Gopčević, K.; Karadžić, I. Cadmium Specific Proteomic  Responses of a Highly Resistant: Pseudomonas Aeruginosa San Ai. RSC Advances 2018, 8  (19), 10549–10560. https://doi.org/10.1039/c8ra00371h" RSC Advances (2018)

The Candida rugosa lipase adsorbed onto titania as nano biocatalyst with improved thermostability and reuse potential in aqueous and organic media

Izrael-Živković, Lidija; Živković, Ljiljana S.; Beškoski, Vladimir; Gopčević, Kristina; Jokić, Bojan; Radosavljević, Dragoslav S.; Karadžić, Ivanka M.

(2016)

TY  - JOUR
AU  - Izrael-Živković, Lidija
AU  - Živković, Ljiljana S.
AU  - Beškoski, Vladimir
AU  - Gopčević, Kristina
AU  - Jokić, Bojan
AU  - Radosavljević, Dragoslav S.
AU  - Karadžić, Ivanka M.
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3648
AB  - The immobilization of Candida rugosa lipase by adsorption was performed onto commercial titania powder (Degussa P25). The change of titania particles surface was diagnosed by means of FTIR and FESEM analysis, as well as by shift of zeta potential value towards that of lipase. A detailed study of the effect of immobilization on enzyme kinetic, temperature stability, as well as on potential for its reuse in aqueous organic media was undertaken. Immobilization of lipase altered enzyme affinity toward substrates with different length of carbon chain in hydrolytic reaction. The Vmax value decreased 2–8-fold, where major constraint was registered for the ester containing the longest carbon chain. Thermostability of lipase improved more than 7-fold at 60 °C. Significant potential for reuse in water solutions was also found after immobilization. In cyclohexane immobilized lipase catalyzed synthesis of amyl octanoate by ping-pong bi–bi mechanism with inhibition by amyl alcohol. Obtained kinetic constants were Vmax = 26.4 μmol min−1, KAc = 0.52 mol/L, KAl = 0.2 mol/L and Ki,Al = 0.644 mol/L. Esterification activity remained 60% after 5 reuse cycles in cyclohexane indicating moderate reuse stability. © 2017 Elsevier B.V.
T2  - Journal of Molecular Catalysis. B: Enzymatic
T1  - The Candida rugosa lipase adsorbed onto titania as nano biocatalyst with improved thermostability and reuse potential in aqueous and organic media
VL  - 133
DO  - 10.1016/j.molcatb.2017.06.001
ER  - 
@article{
author = "Izrael-Živković, Lidija and Živković, Ljiljana S. and Beškoski, Vladimir and Gopčević, Kristina and Jokić, Bojan and Radosavljević, Dragoslav S. and Karadžić, Ivanka M.",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3648",
abstract = "The immobilization of Candida rugosa lipase by adsorption was performed onto commercial titania powder (Degussa P25). The change of titania particles surface was diagnosed by means of FTIR and FESEM analysis, as well as by shift of zeta potential value towards that of lipase. A detailed study of the effect of immobilization on enzyme kinetic, temperature stability, as well as on potential for its reuse in aqueous organic media was undertaken. Immobilization of lipase altered enzyme affinity toward substrates with different length of carbon chain in hydrolytic reaction. The Vmax value decreased 2–8-fold, where major constraint was registered for the ester containing the longest carbon chain. Thermostability of lipase improved more than 7-fold at 60 °C. Significant potential for reuse in water solutions was also found after immobilization. In cyclohexane immobilized lipase catalyzed synthesis of amyl octanoate by ping-pong bi–bi mechanism with inhibition by amyl alcohol. Obtained kinetic constants were Vmax = 26.4 μmol min−1, KAc = 0.52 mol/L, KAl = 0.2 mol/L and Ki,Al = 0.644 mol/L. Esterification activity remained 60% after 5 reuse cycles in cyclohexane indicating moderate reuse stability. © 2017 Elsevier B.V.",
journal = "Journal of Molecular Catalysis. B: Enzymatic",
title = "The Candida rugosa lipase adsorbed onto titania as nano biocatalyst with improved thermostability and reuse potential in aqueous and organic media",
volume = "133",
doi = "10.1016/j.molcatb.2017.06.001"
}
Izrael-Živković, L., Živković, L. S., Beškoski, V., Gopčević, K., Jokić, B., Radosavljević, D. S.,& Karadžić, I. M. (2016). The Candida rugosa lipase adsorbed onto titania as nano biocatalyst with improved thermostability and reuse potential in aqueous and organic media.
Journal of Molecular Catalysis. B: Enzymatic, 133.
https://doi.org/10.1016/j.molcatb.2017.06.001
Izrael-Živković L, Živković LS, Beškoski V, Gopčević K, Jokić B, Radosavljević DS, Karadžić IM. The Candida rugosa lipase adsorbed onto titania as nano biocatalyst with improved thermostability and reuse potential in aqueous and organic media. Journal of Molecular Catalysis. B: Enzymatic. 2016;133
Izrael-Živković Lidija, Živković Ljiljana S., Beškoski Vladimir, Gopčević Kristina, Jokić Bojan, Radosavljević Dragoslav S., Karadžić Ivanka M., "The Candida rugosa lipase adsorbed onto titania as nano biocatalyst with improved thermostability and reuse potential in aqueous and organic media" Journal of Molecular Catalysis. B: Enzymatic, 133 (2016),
https://doi.org/10.1016/j.molcatb.2017.06.001 .
4
3

Supplementary data for the article: Izrael Živković, L. T.; Živković, L. S.; Beškoski, V. P.; Gopčević, K. R.; Jokić, B. M.; Radosavljević, D. S.; Karadžić, I. M. The Candida Rugosa Lipase Adsorbed onto Titania as Nano Biocatalyst with Improved Thermostability and Reuse Potential in Aqueous and Organic Media. Journal of Molecular Catalysis B: Enzymatic 2016, 133, S533–S542. https://doi.org/10.1016/j.molcatb.2017.06.001

Izrael-Živković, Lidija; Živković, Ljiljana S.; Beškoski, Vladimir; Gopčević, Kristina; Jokić, Bojan; Radosavljević, Dragoslav S.; Karadžić, Ivanka M.

(2016)

TY  - BOOK
AU  - Izrael-Živković, Lidija
AU  - Živković, Ljiljana S.
AU  - Beškoski, Vladimir
AU  - Gopčević, Kristina
AU  - Jokić, Bojan
AU  - Radosavljević, Dragoslav S.
AU  - Karadžić, Ivanka M.
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3649
T2  - Journal of Molecular Catalysis. B: Enzymatic
T1  - Supplementary data for the article: Izrael Živković, L. T.; Živković, L. S.; Beškoski, V. P.; Gopčević, K. R.; Jokić, B. M.; Radosavljević, D. S.; Karadžić, I. M. The Candida Rugosa Lipase Adsorbed onto Titania as Nano Biocatalyst with Improved Thermostability and Reuse Potential in Aqueous and Organic Media. Journal of Molecular Catalysis B: Enzymatic 2016, 133, S533–S542. https://doi.org/10.1016/j.molcatb.2017.06.001
ER  - 
@book{
author = "Izrael-Živković, Lidija and Živković, Ljiljana S. and Beškoski, Vladimir and Gopčević, Kristina and Jokić, Bojan and Radosavljević, Dragoslav S. and Karadžić, Ivanka M.",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3649",
journal = "Journal of Molecular Catalysis. B: Enzymatic",
title = "Supplementary data for the article: Izrael Živković, L. T.; Živković, L. S.; Beškoski, V. P.; Gopčević, K. R.; Jokić, B. M.; Radosavljević, D. S.; Karadžić, I. M. The Candida Rugosa Lipase Adsorbed onto Titania as Nano Biocatalyst with Improved Thermostability and Reuse Potential in Aqueous and Organic Media. Journal of Molecular Catalysis B: Enzymatic 2016, 133, S533–S542. https://doi.org/10.1016/j.molcatb.2017.06.001"
}
Izrael-Živković, L., Živković, L. S., Beškoski, V., Gopčević, K., Jokić, B., Radosavljević, D. S.,& Karadžić, I. M. (2016). Supplementary data for the article: Izrael Živković, L. T.; Živković, L. S.; Beškoski, V. P.; Gopčević, K. R.; Jokić, B. M.; Radosavljević, D. S.; Karadžić, I. M. The Candida Rugosa Lipase Adsorbed onto Titania as Nano Biocatalyst with Improved Thermostability and Reuse Potential in Aqueous and Organic Media. Journal of Molecular Catalysis B: Enzymatic 2016, 133, S533–S542. https://doi.org/10.1016/j.molcatb.2017.06.001.
Journal of Molecular Catalysis. B: Enzymatic.
Izrael-Živković L, Živković LS, Beškoski V, Gopčević K, Jokić B, Radosavljević DS, Karadžić IM. Supplementary data for the article: Izrael Živković, L. T.; Živković, L. S.; Beškoski, V. P.; Gopčević, K. R.; Jokić, B. M.; Radosavljević, D. S.; Karadžić, I. M. The Candida Rugosa Lipase Adsorbed onto Titania as Nano Biocatalyst with Improved Thermostability and Reuse Potential in Aqueous and Organic Media. Journal of Molecular Catalysis B: Enzymatic 2016, 133, S533–S542. https://doi.org/10.1016/j.molcatb.2017.06.001. Journal of Molecular Catalysis. B: Enzymatic. 2016;
Izrael-Živković Lidija, Živković Ljiljana S., Beškoski Vladimir, Gopčević Kristina, Jokić Bojan, Radosavljević Dragoslav S., Karadžić Ivanka M., "Supplementary data for the article: Izrael Živković, L. T.; Živković, L. S.; Beškoski, V. P.; Gopčević, K. R.; Jokić, B. M.; Radosavljević, D. S.; Karadžić, I. M. The Candida Rugosa Lipase Adsorbed onto Titania as Nano Biocatalyst with Improved Thermostability and Reuse Potential in Aqueous and Organic Media. Journal of Molecular Catalysis B: Enzymatic 2016, 133, S533–S542. https://doi.org/10.1016/j.molcatb.2017.06.001" Journal of Molecular Catalysis. B: Enzymatic (2016)

The Candida rugosa lipase adsorbed onto titania as nano biocatalyst with improved thermostability and reuse potential in aqueous and organic media

Izrael-Živković, Lidija; Živković, Ljiljana S.; Beškoski, Vladimir; Gopčević, Kristina; Jokić, Bojan; Radosavljević, Dragoslav S.; Karadžić, Ivanka M.

(2016)

TY  - JOUR
AU  - Izrael-Živković, Lidija
AU  - Živković, Ljiljana S.
AU  - Beškoski, Vladimir
AU  - Gopčević, Kristina
AU  - Jokić, Bojan
AU  - Radosavljević, Dragoslav S.
AU  - Karadžić, Ivanka M.
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/315
AB  - The immobilization of Candida rugosa lipase by adsorption was performed onto commercial titania powder (Degussa P25). The change of titania particles surface was diagnosed by means of FTIR and FESEM analysis, as well as by shift of zeta potential value towards that of lipase. A detailed study of the effect of immobilization on enzyme kinetic, temperature stability, as well as on potential for its reuse in aqueous organic media was undertaken. Immobilization of lipase altered enzyme affinity toward substrates with different length of carbon chain in hydrolytic reaction. The Vmax value decreased 2–8-fold, where major constraint was registered for the ester containing the longest carbon chain. Thermostability of lipase improved more than 7-fold at 60 °C. Significant potential for reuse in water solutions was also found after immobilization. In cyclohexane immobilized lipase catalyzed synthesis of amyl octanoate by ping-pong bi–bi mechanism with inhibition by amyl alcohol. Obtained kinetic constants were Vmax = 26.4 μmol min−1, KAc = 0.52 mol/L, KAl = 0.2 mol/L and Ki,Al = 0.644 mol/L. Esterification activity remained 60% after 5 reuse cycles in cyclohexane indicating moderate reuse stability. © 2017 Elsevier B.V.
T2  - Journal of Molecular Catalysis. B: Enzymatic
T1  - The Candida rugosa lipase adsorbed onto titania as nano biocatalyst with improved thermostability and reuse potential in aqueous and organic media
VL  - 133
DO  - 10.1016/j.molcatb.2017.06.001
ER  - 
@article{
author = "Izrael-Živković, Lidija and Živković, Ljiljana S. and Beškoski, Vladimir and Gopčević, Kristina and Jokić, Bojan and Radosavljević, Dragoslav S. and Karadžić, Ivanka M.",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/315",
abstract = "The immobilization of Candida rugosa lipase by adsorption was performed onto commercial titania powder (Degussa P25). The change of titania particles surface was diagnosed by means of FTIR and FESEM analysis, as well as by shift of zeta potential value towards that of lipase. A detailed study of the effect of immobilization on enzyme kinetic, temperature stability, as well as on potential for its reuse in aqueous organic media was undertaken. Immobilization of lipase altered enzyme affinity toward substrates with different length of carbon chain in hydrolytic reaction. The Vmax value decreased 2–8-fold, where major constraint was registered for the ester containing the longest carbon chain. Thermostability of lipase improved more than 7-fold at 60 °C. Significant potential for reuse in water solutions was also found after immobilization. In cyclohexane immobilized lipase catalyzed synthesis of amyl octanoate by ping-pong bi–bi mechanism with inhibition by amyl alcohol. Obtained kinetic constants were Vmax = 26.4 μmol min−1, KAc = 0.52 mol/L, KAl = 0.2 mol/L and Ki,Al = 0.644 mol/L. Esterification activity remained 60% after 5 reuse cycles in cyclohexane indicating moderate reuse stability. © 2017 Elsevier B.V.",
journal = "Journal of Molecular Catalysis. B: Enzymatic",
title = "The Candida rugosa lipase adsorbed onto titania as nano biocatalyst with improved thermostability and reuse potential in aqueous and organic media",
volume = "133",
doi = "10.1016/j.molcatb.2017.06.001"
}
Izrael-Živković, L., Živković, L. S., Beškoski, V., Gopčević, K., Jokić, B., Radosavljević, D. S.,& Karadžić, I. M. (2016). The Candida rugosa lipase adsorbed onto titania as nano biocatalyst with improved thermostability and reuse potential in aqueous and organic media.
Journal of Molecular Catalysis. B: Enzymatic, 133.
https://doi.org/10.1016/j.molcatb.2017.06.001
Izrael-Živković L, Živković LS, Beškoski V, Gopčević K, Jokić B, Radosavljević DS, Karadžić IM. The Candida rugosa lipase adsorbed onto titania as nano biocatalyst with improved thermostability and reuse potential in aqueous and organic media. Journal of Molecular Catalysis. B: Enzymatic. 2016;133
Izrael-Živković Lidija, Živković Ljiljana S., Beškoski Vladimir, Gopčević Kristina, Jokić Bojan, Radosavljević Dragoslav S., Karadžić Ivanka M., "The Candida rugosa lipase adsorbed onto titania as nano biocatalyst with improved thermostability and reuse potential in aqueous and organic media" Journal of Molecular Catalysis. B: Enzymatic, 133 (2016),
https://doi.org/10.1016/j.molcatb.2017.06.001 .
4
3