@article{
author = "Ljoljić Bilić, Vanja and Gašić, Uroš M. and Milojković-Opsenica, Dušanka and Rimac, Hrvoje and Vuković Rodriguez, Jadranka and Vlainić, Josipa and Brlek‐Gorski, Diana and Kosalec, Ivan",
year = "2022",
abstract = "Followed by a buildup of its phytochemical profile, Erodium cicutarium is being subjected
to antimicrobial investigation guided with its ethnobotanical use. The results of performed in vitro
screening on Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans strains, show that
E. cicutarium has antimicrobial activity, with a particular emphasis on clinical S. aureus strains—
both the methicillin sensitive (MSSA) and the methicillin resistant (MRSA) S. aureus. Experimental
design consisted of general methods (the serial microdilution broth assay and the agar well diffusion
assay), as well as observing bactericidal/bacteriostatic activity through time (the “time‐kill”
assay), investigating the effect on cell wall integrity and biofilm formation, and modulation of bacterial
hemolysis. Observed antibacterial activity from above‐described methods led to further activity‐
guided fractionation of water and methanol extracts using bioautography coupled with UHPLCLTQ
OrbiTrap MS4. It was determined that active fractions are predominantly formed by gallic acid
derivatives and flavonol glycosides. Among the most active phytochemicals, galloyl‐shikimic acid
was identified as the most abundant compound. These results point to a direct connection between
galloyl‐shikimic acid and the observed E. cicutarium antibacterial activity, and open several new
research approaches for future investigation",
publisher = "MDPI",
journal = "Аntibiotics",
title = "Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus",
volume = "11",
pages = "492",
doi = "10.3390/antibiotics11040492"
}