Radotić, Ksenija

Link to this page

Authority KeyName Variants
orcid::0000-0002-9770-0788
  • Radotić, Ksenija (32)
Projects
Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance
Synthesis, processing and characterization of nanostructured materials for application in the field of energy, mechanical engineering, environmental protection and biomedicine Structure-properties relationships of natural and synthetic molecules and their metal complexes
The membranes as sites of interaction between the intracellular and apoplastic environments: studies of the bioenergetics and signaling using biophysical and biochemical techniques. Simultaneous Bioremediation and Soilification of Degraded Areas to Preserve Natural Resources of Biologically Active Substances, and Development and Production of Biomaterials and Dietetic Products
Ispitivanja novih biosenzora za monitoring i dijagnostiku biljaka Natural products of wild, cultivated and edible plants: structure and bioactivity determination
Characterization and application of fungal metabolites and assessment of new biofungicides potential Develooment and utilization of novel and traditional technologies in production of competitive food products with added valued for national and global market - CREATING WEALTH FROM THE WEALTH OF SERBIA
ARDITI-Agência Regional para o Desenvolvimento da Investigação Tecnologia e Inovação, project M1420-01-0145-FEDER-000005 - Centro de Química da Madeira - CQM+ Fishes as water quality indicators in open waters of Serbia
Biomarkers in neurodegenerative and malignant processes project RTI2018-099668-BC22 of Ministerio de Ciencia, Innovación y Universidades
Conservation strategy for protected and strictly protected hoverflies (Insecta: Diptera: Syrphidae) species in Serbia - Case study Advanced technologies for monitoring and environmental protection from chemical pollutants and radiation burden

Author's Bibliography

Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose–response relationship

Bartolić, Dragana; Maksimović, Vuk; Maksimović, Jelena D.; Stanković, Mira; Krstović, Saša; Baošić, Rada; Radotić, Ksenija

(Wiley, 2020)

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Maksimović, Vuk
AU  - Maksimović, Jelena D.
AU  - Stanković, Mira
AU  - Krstović, Saša
AU  - Baošić, Rada
AU  - Radotić, Ksenija
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3949
AB  - BACKGROUND: Cereal seeds, such as maize seeds, are frequently contaminated with aflatoxin B1 (AFB1), one of the most dangerous naturally occurring carcinogens. In plants, phenolamides are involved in biotic stress response. The data on variations of phenolamides in AFB1-containing seeds are limited. RESULTS: Five polyamine conjugates, including two spermidine and three putrescine conjugates, were tentatively identified in methanolic extracts, using HPLC-DAD-MS. The ratio of putrescine to spermidine conjugates changed with increasing AFB1 concentration in a logistic dose–response manner, with a ratio of below 1 up to a concentration of 51.51 μg kg−1, and approximately 2.54 and 3 at higher concentrations of 177.4 and 308.13 μg kg−1, respectively. The observed variations of the total antioxidant activity and the total phenolic content may support this biphasic behaviour of the seeds against AFB1 stress. CONCLUSIONS: The obtained data are a contribution to the understanding of the roles of polyamine conjugates in seed defence to increasing AFB1 concentrations. According to our knowledge, this study reports for the first time the biphasic response of maize seeds to increasing AFB1 contamination level, comprising the induction of polyamine conjugate accumulation and variation in the ratio of conjugates. This dose–response relationship may provide useful information in the field of agricultural and food chemistry as an indicator of AFB1 contamination level and, hence, for selecting an appropriate seed quality.
PB  - Wiley
T2  - Journal of the Science of Food and Agriculture
T1  - Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose–response relationship
VL  - 100
IS  - 7
SP  - 2905
EP  - 2910
DO  - 10.1002/jsfa.10317
ER  - 
@article{
author = "Bartolić, Dragana and Maksimović, Vuk and Maksimović, Jelena D. and Stanković, Mira and Krstović, Saša and Baošić, Rada and Radotić, Ksenija",
year = "2020",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3949",
abstract = "BACKGROUND: Cereal seeds, such as maize seeds, are frequently contaminated with aflatoxin B1 (AFB1), one of the most dangerous naturally occurring carcinogens. In plants, phenolamides are involved in biotic stress response. The data on variations of phenolamides in AFB1-containing seeds are limited. RESULTS: Five polyamine conjugates, including two spermidine and three putrescine conjugates, were tentatively identified in methanolic extracts, using HPLC-DAD-MS. The ratio of putrescine to spermidine conjugates changed with increasing AFB1 concentration in a logistic dose–response manner, with a ratio of below 1 up to a concentration of 51.51 μg kg−1, and approximately 2.54 and 3 at higher concentrations of 177.4 and 308.13 μg kg−1, respectively. The observed variations of the total antioxidant activity and the total phenolic content may support this biphasic behaviour of the seeds against AFB1 stress. CONCLUSIONS: The obtained data are a contribution to the understanding of the roles of polyamine conjugates in seed defence to increasing AFB1 concentrations. According to our knowledge, this study reports for the first time the biphasic response of maize seeds to increasing AFB1 contamination level, comprising the induction of polyamine conjugate accumulation and variation in the ratio of conjugates. This dose–response relationship may provide useful information in the field of agricultural and food chemistry as an indicator of AFB1 contamination level and, hence, for selecting an appropriate seed quality.",
publisher = "Wiley",
journal = "Journal of the Science of Food and Agriculture",
title = "Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose–response relationship",
volume = "100",
number = "7",
pages = "2905-2910",
doi = "10.1002/jsfa.10317"
}
Bartolić, D., Maksimović, V., Maksimović, J. D., Stanković, M., Krstović, S., Baošić, R.,& Radotić, K. (2020). Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose–response relationship.
Journal of the Science of Food and Agriculture
Wiley., 100(7), 2905-2910.
https://doi.org/10.1002/jsfa.10317
Bartolić D, Maksimović V, Maksimović JD, Stanković M, Krstović S, Baošić R, Radotić K. Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose–response relationship. Journal of the Science of Food and Agriculture. 2020;100(7):2905-2910
Bartolić Dragana, Maksimović Vuk, Maksimović Jelena D., Stanković Mira, Krstović Saša, Baošić Rada, Radotić Ksenija, "Variations in polyamine conjugates in maize (Zea mays L.) seeds contaminated with aflatoxin B1: a dose–response relationship" Journal of the Science of Food and Agriculture, 100, no. 7 (2020):2905-2910,
https://doi.org/10.1002/jsfa.10317 .
2
2

Peroxidase-Sensitive Tyramine Carboxymethyl Xylan Hydrogels for Enzyme Encapsulation

Spasojević, Dragica; Prokopijević, Miloš; Prodanović, Olivera; Zelenović, Nevena D.; Polović, Natalija; Radotić, Ksenija; Prodanović, Radivoje

(The Polymer Society of Korea, 2019)

TY  - JOUR
AU  - Spasojević, Dragica
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Zelenović, Nevena D.
AU  - Polović, Natalija
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3732
AB  - Derivatives of xylans were synthesized from corncob xylan by carboxymethylation, oxidization with different molar ratios of periodate (5, 10 15 and 20 mol%) and by reductive amination with tyramine. Modifications of tyramine carboxymethyl xylans (Tyr-CMX) were confirmed by FTIR, UV and NMR spectra. Concentration of ionizable groups increased from 1.5 mmol/g for carboxymethyl xylan (CMX) to 5.4 mmol/g for Tyr-CMX oxidized with 20 mol% of periodate. All Tyr-CMXs were able to form hydrogels the cross-linking reaction with horseradish peroxidase and peroxide. Tyr-CMXs were tested for amyloglucosidase (AG) encapsulation within hydrogel microbeads obtained in a reaction of emulsion polymerization with peroxidase. Average diameter of Tyr-CMX hydrogel microbeads was 52±25 µm and after encapsulation optimization with respect to the extent of CMX modification with tyramine, the concentration of Tyr-CMX, and the amount of added AG, microbeads with AG specific activity of 2 U/mL and 20% yield of immobilization were obtained. The optimum pH of the immobilized AG was not changed compared to the soluble one, while half-life at 60 °C was increased around 10 times. The Michaelis-Menten constant for the immobilized enzyme, 1.03 mM, was significantly lower than that for the soluble one, 1.54 mM. After 5 cycles of repetitive use in batch reactor, the immobilized AG retained 68% of initial activity.
PB  - The Polymer Society of Korea
T2  - Macromolecular Research
T1  - Peroxidase-Sensitive Tyramine Carboxymethyl Xylan Hydrogels for Enzyme Encapsulation
VL  - 27
IS  - 8
SP  - 764
EP  - 771
DO  - 10.1007/s13233-019-7111-7
ER  - 
@article{
author = "Spasojević, Dragica and Prokopijević, Miloš and Prodanović, Olivera and Zelenović, Nevena D. and Polović, Natalija and Radotić, Ksenija and Prodanović, Radivoje",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3732",
abstract = "Derivatives of xylans were synthesized from corncob xylan by carboxymethylation, oxidization with different molar ratios of periodate (5, 10 15 and 20 mol%) and by reductive amination with tyramine. Modifications of tyramine carboxymethyl xylans (Tyr-CMX) were confirmed by FTIR, UV and NMR spectra. Concentration of ionizable groups increased from 1.5 mmol/g for carboxymethyl xylan (CMX) to 5.4 mmol/g for Tyr-CMX oxidized with 20 mol% of periodate. All Tyr-CMXs were able to form hydrogels the cross-linking reaction with horseradish peroxidase and peroxide. Tyr-CMXs were tested for amyloglucosidase (AG) encapsulation within hydrogel microbeads obtained in a reaction of emulsion polymerization with peroxidase. Average diameter of Tyr-CMX hydrogel microbeads was 52±25 µm and after encapsulation optimization with respect to the extent of CMX modification with tyramine, the concentration of Tyr-CMX, and the amount of added AG, microbeads with AG specific activity of 2 U/mL and 20% yield of immobilization were obtained. The optimum pH of the immobilized AG was not changed compared to the soluble one, while half-life at 60 °C was increased around 10 times. The Michaelis-Menten constant for the immobilized enzyme, 1.03 mM, was significantly lower than that for the soluble one, 1.54 mM. After 5 cycles of repetitive use in batch reactor, the immobilized AG retained 68% of initial activity.",
publisher = "The Polymer Society of Korea",
journal = "Macromolecular Research",
title = "Peroxidase-Sensitive Tyramine Carboxymethyl Xylan Hydrogels for Enzyme Encapsulation",
volume = "27",
number = "8",
pages = "764-771",
doi = "10.1007/s13233-019-7111-7"
}
Spasojević, D., Prokopijević, M., Prodanović, O., Zelenović, N. D., Polović, N., Radotić, K.,& Prodanović, R. (2019). Peroxidase-Sensitive Tyramine Carboxymethyl Xylan Hydrogels for Enzyme Encapsulation.
Macromolecular Research
The Polymer Society of Korea., 27(8), 764-771.
https://doi.org/10.1007/s13233-019-7111-7
Spasojević D, Prokopijević M, Prodanović O, Zelenović ND, Polović N, Radotić K, Prodanović R. Peroxidase-Sensitive Tyramine Carboxymethyl Xylan Hydrogels for Enzyme Encapsulation. Macromolecular Research. 2019;27(8):764-771
Spasojević Dragica, Prokopijević Miloš, Prodanović Olivera, Zelenović Nevena D., Polović Natalija, Radotić Ksenija, Prodanović Radivoje, "Peroxidase-Sensitive Tyramine Carboxymethyl Xylan Hydrogels for Enzyme Encapsulation" Macromolecular Research, 27, no. 8 (2019):764-771,
https://doi.org/10.1007/s13233-019-7111-7 .
3
2
3

Supplementary data for the article: Smailagić, A.; Veljović, S.; Gašić, U. M.; Dabić-Zagorac, D.; Stanković, M.; Radotić, K.; Natić, M. Phenolic Profile, Chromatic Parameters and Fluorescence of Different Woods Used in Balkan Cooperage. Industrial Crops and Products 2019, 132, 156–167. https://doi.org/10.1016/j.indcrop.2019.02.017

Smailagić, Anita; Veljović, Sonja; Gašić, Uroš M.; Dabić-Zagorac, Dragana; Stanković, Mira; Radotić, Ksenija; Natić, Maja

(Elsevier, 2019)

TY  - BOOK
AU  - Smailagić, Anita
AU  - Veljović, Sonja
AU  - Gašić, Uroš M.
AU  - Dabić-Zagorac, Dragana
AU  - Stanković, Mira
AU  - Radotić, Ksenija
AU  - Natić, Maja
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3921
PB  - Elsevier
T2  - Industrial Crops and Products
T1  - Supplementary data for the article: Smailagić, A.; Veljović, S.; Gašić, U. M.; Dabić-Zagorac, D.; Stanković, M.; Radotić, K.; Natić, M. Phenolic Profile, Chromatic Parameters and Fluorescence of Different Woods Used in Balkan Cooperage. Industrial Crops and Products 2019, 132, 156–167. https://doi.org/10.1016/j.indcrop.2019.02.017
ER  - 
@book{
author = "Smailagić, Anita and Veljović, Sonja and Gašić, Uroš M. and Dabić-Zagorac, Dragana and Stanković, Mira and Radotić, Ksenija and Natić, Maja",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3921",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "Supplementary data for the article: Smailagić, A.; Veljović, S.; Gašić, U. M.; Dabić-Zagorac, D.; Stanković, M.; Radotić, K.; Natić, M. Phenolic Profile, Chromatic Parameters and Fluorescence of Different Woods Used in Balkan Cooperage. Industrial Crops and Products 2019, 132, 156–167. https://doi.org/10.1016/j.indcrop.2019.02.017"
}
Smailagić, A., Veljović, S., Gašić, U. M., Dabić-Zagorac, D., Stanković, M., Radotić, K.,& Natić, M. (2019). Supplementary data for the article: Smailagić, A.; Veljović, S.; Gašić, U. M.; Dabić-Zagorac, D.; Stanković, M.; Radotić, K.; Natić, M. Phenolic Profile, Chromatic Parameters and Fluorescence of Different Woods Used in Balkan Cooperage. Industrial Crops and Products 2019, 132, 156–167. https://doi.org/10.1016/j.indcrop.2019.02.017.
Industrial Crops and Products
Elsevier..
Smailagić A, Veljović S, Gašić UM, Dabić-Zagorac D, Stanković M, Radotić K, Natić M. Supplementary data for the article: Smailagić, A.; Veljović, S.; Gašić, U. M.; Dabić-Zagorac, D.; Stanković, M.; Radotić, K.; Natić, M. Phenolic Profile, Chromatic Parameters and Fluorescence of Different Woods Used in Balkan Cooperage. Industrial Crops and Products 2019, 132, 156–167. https://doi.org/10.1016/j.indcrop.2019.02.017. Industrial Crops and Products. 2019;
Smailagić Anita, Veljović Sonja, Gašić Uroš M., Dabić-Zagorac Dragana, Stanković Mira, Radotić Ksenija, Natić Maja, "Supplementary data for the article: Smailagić, A.; Veljović, S.; Gašić, U. M.; Dabić-Zagorac, D.; Stanković, M.; Radotić, K.; Natić, M. Phenolic Profile, Chromatic Parameters and Fluorescence of Different Woods Used in Balkan Cooperage. Industrial Crops and Products 2019, 132, 156–167. https://doi.org/10.1016/j.indcrop.2019.02.017" Industrial Crops and Products (2019)

Phenolic profile, chromatic parameters and fluorescence of different woods used in Balkan cooperage

Smailagić, Anita; Veljović, Sonja; Gašić, Uroš M.; Dabić-Zagorac, Dragana; Stanković, Mira; Radotić, Ksenija; Natić, Maja

(Elsevier, 2019)

TY  - JOUR
AU  - Smailagić, Anita
AU  - Veljović, Sonja
AU  - Gašić, Uroš M.
AU  - Dabić-Zagorac, Dragana
AU  - Stanković, Mira
AU  - Radotić, Ksenija
AU  - Natić, Maja
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2832
AB  - The aim of this research was to study phenolic compounds of diverse botanical species of wood commonly used in cooperage in Balkan countries. Several botanical species have been considered including mulberry (Morus alba L.), myrobalan plum (Prunus cerasifera Ehrh.), black locust (Robinia pseudoacacia L.), wild cherry (Prunus avium (L.) L.), and oak (Q. petraea (Matt.) Liebl., Q. robur L., and Q. cerris L.). A total of 37 compounds were quantified, demonstrating the presence of phenolic acids, flavonols, flavones, flavanones, flavanonol taxifolin, stilbenoids, and coumarins. Taxifolin was the most abundant in wild cherry (8455.70 mg kg−1), while ellagic acid predominated in oak wood (8872.05–10099.32 mg kg−1 in sessile oaks, and up to 15,958.80 mg kg−1 in pedunculate oak from Slavonia). The highest content of protocatechuic acid (533.39 mg kg−1) was found in myrobalan plum. Also, isoflavones were characteristic of wild cherry, while mulberry was abundant in stilbenoids. Total phenolic content, as well as antioxidant, chromatic, and fluorescence properties were studied. The spectral shapes and maxima of fluorescence emission spectra of bare wood samples were compared with those of the corresponding wood extracts. The Principal Component Analysis (PCA) was applied in order to find patterns in emission spectra for differentiation among wood samples.
PB  - Elsevier
T2  - Industrial Crops and Products
T1  - Phenolic profile, chromatic parameters and fluorescence of different woods used in Balkan cooperage
VL  - 132
SP  - 156
EP  - 167
DO  - 10.1016/j.indcrop.2019.02.017
ER  - 
@article{
author = "Smailagić, Anita and Veljović, Sonja and Gašić, Uroš M. and Dabić-Zagorac, Dragana and Stanković, Mira and Radotić, Ksenija and Natić, Maja",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2832",
abstract = "The aim of this research was to study phenolic compounds of diverse botanical species of wood commonly used in cooperage in Balkan countries. Several botanical species have been considered including mulberry (Morus alba L.), myrobalan plum (Prunus cerasifera Ehrh.), black locust (Robinia pseudoacacia L.), wild cherry (Prunus avium (L.) L.), and oak (Q. petraea (Matt.) Liebl., Q. robur L., and Q. cerris L.). A total of 37 compounds were quantified, demonstrating the presence of phenolic acids, flavonols, flavones, flavanones, flavanonol taxifolin, stilbenoids, and coumarins. Taxifolin was the most abundant in wild cherry (8455.70 mg kg−1), while ellagic acid predominated in oak wood (8872.05–10099.32 mg kg−1 in sessile oaks, and up to 15,958.80 mg kg−1 in pedunculate oak from Slavonia). The highest content of protocatechuic acid (533.39 mg kg−1) was found in myrobalan plum. Also, isoflavones were characteristic of wild cherry, while mulberry was abundant in stilbenoids. Total phenolic content, as well as antioxidant, chromatic, and fluorescence properties were studied. The spectral shapes and maxima of fluorescence emission spectra of bare wood samples were compared with those of the corresponding wood extracts. The Principal Component Analysis (PCA) was applied in order to find patterns in emission spectra for differentiation among wood samples.",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "Phenolic profile, chromatic parameters and fluorescence of different woods used in Balkan cooperage",
volume = "132",
pages = "156-167",
doi = "10.1016/j.indcrop.2019.02.017"
}
Smailagić, A., Veljović, S., Gašić, U. M., Dabić-Zagorac, D., Stanković, M., Radotić, K.,& Natić, M. (2019). Phenolic profile, chromatic parameters and fluorescence of different woods used in Balkan cooperage.
Industrial Crops and Products
Elsevier., 132, 156-167.
https://doi.org/10.1016/j.indcrop.2019.02.017
Smailagić A, Veljović S, Gašić UM, Dabić-Zagorac D, Stanković M, Radotić K, Natić M. Phenolic profile, chromatic parameters and fluorescence of different woods used in Balkan cooperage. Industrial Crops and Products. 2019;132:156-167
Smailagić Anita, Veljović Sonja, Gašić Uroš M., Dabić-Zagorac Dragana, Stanković Mira, Radotić Ksenija, Natić Maja, "Phenolic profile, chromatic parameters and fluorescence of different woods used in Balkan cooperage" Industrial Crops and Products, 132 (2019):156-167,
https://doi.org/10.1016/j.indcrop.2019.02.017 .
7
6
6

Phenolic profile, chromatic parameters and fluorescence of different woods used in Balkan cooperage

Smailagić, Anita; Veljović, Sonja; Gašić, Uroš M.; Dabić-Zagorac, Dragana; Stanković, Mira; Radotić, Ksenija; Natić, Maja

(Elsevier, 2019)

TY  - JOUR
AU  - Smailagić, Anita
AU  - Veljović, Sonja
AU  - Gašić, Uroš M.
AU  - Dabić-Zagorac, Dragana
AU  - Stanković, Mira
AU  - Radotić, Ksenija
AU  - Natić, Maja
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2835
AB  - The aim of this research was to study phenolic compounds of diverse botanical species of wood commonly used in cooperage in Balkan countries. Several botanical species have been considered including mulberry (Morus alba L.), myrobalan plum (Prunus cerasifera Ehrh.), black locust (Robinia pseudoacacia L.), wild cherry (Prunus avium (L.) L.), and oak (Q. petraea (Matt.) Liebl., Q. robur L., and Q. cerris L.). A total of 37 compounds were quantified, demonstrating the presence of phenolic acids, flavonols, flavones, flavanones, flavanonol taxifolin, stilbenoids, and coumarins. Taxifolin was the most abundant in wild cherry (8455.70 mg kg−1), while ellagic acid predominated in oak wood (8872.05–10099.32 mg kg−1 in sessile oaks, and up to 15,958.80 mg kg−1 in pedunculate oak from Slavonia). The highest content of protocatechuic acid (533.39 mg kg−1) was found in myrobalan plum. Also, isoflavones were characteristic of wild cherry, while mulberry was abundant in stilbenoids. Total phenolic content, as well as antioxidant, chromatic, and fluorescence properties were studied. The spectral shapes and maxima of fluorescence emission spectra of bare wood samples were compared with those of the corresponding wood extracts. The Principal Component Analysis (PCA) was applied in order to find patterns in emission spectra for differentiation among wood samples.
PB  - Elsevier
T2  - Industrial Crops and Products
T1  - Phenolic profile, chromatic parameters and fluorescence of different woods used in Balkan cooperage
VL  - 132
SP  - 156
EP  - 167
DO  - 10.1016/j.indcrop.2019.02.017
ER  - 
@article{
author = "Smailagić, Anita and Veljović, Sonja and Gašić, Uroš M. and Dabić-Zagorac, Dragana and Stanković, Mira and Radotić, Ksenija and Natić, Maja",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2835",
abstract = "The aim of this research was to study phenolic compounds of diverse botanical species of wood commonly used in cooperage in Balkan countries. Several botanical species have been considered including mulberry (Morus alba L.), myrobalan plum (Prunus cerasifera Ehrh.), black locust (Robinia pseudoacacia L.), wild cherry (Prunus avium (L.) L.), and oak (Q. petraea (Matt.) Liebl., Q. robur L., and Q. cerris L.). A total of 37 compounds were quantified, demonstrating the presence of phenolic acids, flavonols, flavones, flavanones, flavanonol taxifolin, stilbenoids, and coumarins. Taxifolin was the most abundant in wild cherry (8455.70 mg kg−1), while ellagic acid predominated in oak wood (8872.05–10099.32 mg kg−1 in sessile oaks, and up to 15,958.80 mg kg−1 in pedunculate oak from Slavonia). The highest content of protocatechuic acid (533.39 mg kg−1) was found in myrobalan plum. Also, isoflavones were characteristic of wild cherry, while mulberry was abundant in stilbenoids. Total phenolic content, as well as antioxidant, chromatic, and fluorescence properties were studied. The spectral shapes and maxima of fluorescence emission spectra of bare wood samples were compared with those of the corresponding wood extracts. The Principal Component Analysis (PCA) was applied in order to find patterns in emission spectra for differentiation among wood samples.",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "Phenolic profile, chromatic parameters and fluorescence of different woods used in Balkan cooperage",
volume = "132",
pages = "156-167",
doi = "10.1016/j.indcrop.2019.02.017"
}
Smailagić, A., Veljović, S., Gašić, U. M., Dabić-Zagorac, D., Stanković, M., Radotić, K.,& Natić, M. (2019). Phenolic profile, chromatic parameters and fluorescence of different woods used in Balkan cooperage.
Industrial Crops and Products
Elsevier., 132, 156-167.
https://doi.org/10.1016/j.indcrop.2019.02.017
Smailagić A, Veljović S, Gašić UM, Dabić-Zagorac D, Stanković M, Radotić K, Natić M. Phenolic profile, chromatic parameters and fluorescence of different woods used in Balkan cooperage. Industrial Crops and Products. 2019;132:156-167
Smailagić Anita, Veljović Sonja, Gašić Uroš M., Dabić-Zagorac Dragana, Stanković Mira, Radotić Ksenija, Natić Maja, "Phenolic profile, chromatic parameters and fluorescence of different woods used in Balkan cooperage" Industrial Crops and Products, 132 (2019):156-167,
https://doi.org/10.1016/j.indcrop.2019.02.017 .
7
6
6

Interaction of carbohydrate coated cerium-oxide nanoparticles with wheat and pea: Stress induction potential and effect on development

Milenković, Ivana; Mitrović, Aleksandra D.; Algarra, Manuel; Lázaro-Martínez, Juan M.; Rodríguez-Castellón, Enrique; Maksimović, Vuk; Spasić, Slađana Z.; Beškoski, Vladimir; Radotić, Ksenija

(MDPI, 2019)

TY  - JOUR
AU  - Milenković, Ivana
AU  - Mitrović, Aleksandra D.
AU  - Algarra, Manuel
AU  - Lázaro-Martínez, Juan M.
AU  - Rodríguez-Castellón, Enrique
AU  - Maksimović, Vuk
AU  - Spasić, Slađana Z.
AU  - Beškoski, Vladimir
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3698
AB  - Reports about the influence of cerium-oxide nanoparticles (nCeO2) on plants are contradictory due to their positive and negative effects on plants. Surface modification may affect the interaction of nCeO2 with the environment, and hence its availability to plants. In this study, the uncoated and glucose-, levan-, and pullulan-coated nCeO2 were synthesized and characterized. The aim was to determine whether nontoxic carbohydrates alter the effect of nCeO2 on the seed germination, plant growth, and metabolism of wheat and pea. We applied 200 mgL-1 of nCeO2 on plants during germination (Ger treatment) or three week-growth (Gro treatment) in hydroponics. The plant response to nCeO2 was studied by measuring changes in Ce concentration, total antioxidative activity (TAA), total phenolic content (TPC), and phenolic profile. Our results generally revealed higher Ce concentration in plants after the treatment with coated nanoparticles compared to uncoated ones. Considering all obtained results, Ger treatment had a stronger impact on the later stages of plant development than Gro treatment. The Ger treatment had a stronger impact on TPC and plant elongation, whereas Gro treatment affected more TAA and phenolic profile. Among nanoparticles, levan-coated nCeO2 had the strongest and positive impact on tested plants. Wheat showed higher sensitivity to all treatments.
PB  - MDPI
T2  - Plants
T1  - Interaction of carbohydrate coated cerium-oxide nanoparticles with wheat and pea: Stress induction potential and effect on development
VL  - 8
IS  - 11
DO  - 10.3390/plants8110478
ER  - 
@article{
author = "Milenković, Ivana and Mitrović, Aleksandra D. and Algarra, Manuel and Lázaro-Martínez, Juan M. and Rodríguez-Castellón, Enrique and Maksimović, Vuk and Spasić, Slađana Z. and Beškoski, Vladimir and Radotić, Ksenija",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3698",
abstract = "Reports about the influence of cerium-oxide nanoparticles (nCeO2) on plants are contradictory due to their positive and negative effects on plants. Surface modification may affect the interaction of nCeO2 with the environment, and hence its availability to plants. In this study, the uncoated and glucose-, levan-, and pullulan-coated nCeO2 were synthesized and characterized. The aim was to determine whether nontoxic carbohydrates alter the effect of nCeO2 on the seed germination, plant growth, and metabolism of wheat and pea. We applied 200 mgL-1 of nCeO2 on plants during germination (Ger treatment) or three week-growth (Gro treatment) in hydroponics. The plant response to nCeO2 was studied by measuring changes in Ce concentration, total antioxidative activity (TAA), total phenolic content (TPC), and phenolic profile. Our results generally revealed higher Ce concentration in plants after the treatment with coated nanoparticles compared to uncoated ones. Considering all obtained results, Ger treatment had a stronger impact on the later stages of plant development than Gro treatment. The Ger treatment had a stronger impact on TPC and plant elongation, whereas Gro treatment affected more TAA and phenolic profile. Among nanoparticles, levan-coated nCeO2 had the strongest and positive impact on tested plants. Wheat showed higher sensitivity to all treatments.",
publisher = "MDPI",
journal = "Plants",
title = "Interaction of carbohydrate coated cerium-oxide nanoparticles with wheat and pea: Stress induction potential and effect on development",
volume = "8",
number = "11",
doi = "10.3390/plants8110478"
}
Milenković, I., Mitrović, A. D., Algarra, M., Lázaro-Martínez, J. M., Rodríguez-Castellón, E., Maksimović, V., Spasić, S. Z., Beškoski, V.,& Radotić, K. (2019). Interaction of carbohydrate coated cerium-oxide nanoparticles with wheat and pea: Stress induction potential and effect on development.
Plants
MDPI., 8(11).
https://doi.org/10.3390/plants8110478
Milenković I, Mitrović AD, Algarra M, Lázaro-Martínez JM, Rodríguez-Castellón E, Maksimović V, Spasić SZ, Beškoski V, Radotić K. Interaction of carbohydrate coated cerium-oxide nanoparticles with wheat and pea: Stress induction potential and effect on development. Plants. 2019;8(11)
Milenković Ivana, Mitrović Aleksandra D., Algarra Manuel, Lázaro-Martínez Juan M., Rodríguez-Castellón Enrique, Maksimović Vuk, Spasić Slađana Z., Beškoski Vladimir, Radotić Ksenija, "Interaction of carbohydrate coated cerium-oxide nanoparticles with wheat and pea: Stress induction potential and effect on development" Plants, 8, no. 11 (2019),
https://doi.org/10.3390/plants8110478 .
4
1
3

Supplementary data for the article: Milenković, I.; Mitrović, A.; Algarra, M.; Lázaro-Martínez, J. M.; Rodríguez-Castellón, E.; Maksimović, V.; Spasić, S. Z.; Beškoski, V. P.; Radotić, K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. Plants 2019, 8 (11). https://doi.org/10.3390/plants8110478

Milenković, Ivana; Mitrović, Aleksandra D.; Algarra, Manuel; Lázaro-Martínez, Juan M.; Rodríguez-Castellón, Enrique; Maksimović, Vuk; Spasić, Slađana Z.; Beškoski, Vladimir; Radotić, Ksenija

(MDPI, 2019)

TY  - BOOK
AU  - Milenković, Ivana
AU  - Mitrović, Aleksandra D.
AU  - Algarra, Manuel
AU  - Lázaro-Martínez, Juan M.
AU  - Rodríguez-Castellón, Enrique
AU  - Maksimović, Vuk
AU  - Spasić, Slađana Z.
AU  - Beškoski, Vladimir
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3699
PB  - MDPI
T2  - Plants
T1  - Supplementary data for the article: Milenković, I.; Mitrović, A.; Algarra, M.; Lázaro-Martínez, J. M.; Rodríguez-Castellón, E.; Maksimović, V.; Spasić, S. Z.; Beškoski, V. P.; Radotić, K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. Plants 2019, 8 (11). https://doi.org/10.3390/plants8110478
ER  - 
@book{
author = "Milenković, Ivana and Mitrović, Aleksandra D. and Algarra, Manuel and Lázaro-Martínez, Juan M. and Rodríguez-Castellón, Enrique and Maksimović, Vuk and Spasić, Slađana Z. and Beškoski, Vladimir and Radotić, Ksenija",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3699",
publisher = "MDPI",
journal = "Plants",
title = "Supplementary data for the article: Milenković, I.; Mitrović, A.; Algarra, M.; Lázaro-Martínez, J. M.; Rodríguez-Castellón, E.; Maksimović, V.; Spasić, S. Z.; Beškoski, V. P.; Radotić, K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. Plants 2019, 8 (11). https://doi.org/10.3390/plants8110478"
}
Milenković, I., Mitrović, A. D., Algarra, M., Lázaro-Martínez, J. M., Rodríguez-Castellón, E., Maksimović, V., Spasić, S. Z., Beškoski, V.,& Radotić, K. (2019). Supplementary data for the article: Milenković, I.; Mitrović, A.; Algarra, M.; Lázaro-Martínez, J. M.; Rodríguez-Castellón, E.; Maksimović, V.; Spasić, S. Z.; Beškoski, V. P.; Radotić, K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. Plants 2019, 8 (11). https://doi.org/10.3390/plants8110478.
Plants
MDPI..
Milenković I, Mitrović AD, Algarra M, Lázaro-Martínez JM, Rodríguez-Castellón E, Maksimović V, Spasić SZ, Beškoski V, Radotić K. Supplementary data for the article: Milenković, I.; Mitrović, A.; Algarra, M.; Lázaro-Martínez, J. M.; Rodríguez-Castellón, E.; Maksimović, V.; Spasić, S. Z.; Beškoski, V. P.; Radotić, K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. Plants 2019, 8 (11). https://doi.org/10.3390/plants8110478. Plants. 2019;
Milenković Ivana, Mitrović Aleksandra D., Algarra Manuel, Lázaro-Martínez Juan M., Rodríguez-Castellón Enrique, Maksimović Vuk, Spasić Slađana Z., Beškoski Vladimir, Radotić Ksenija, "Supplementary data for the article: Milenković, I.; Mitrović, A.; Algarra, M.; Lázaro-Martínez, J. M.; Rodríguez-Castellón, E.; Maksimović, V.; Spasić, S. Z.; Beškoski, V. P.; Radotić, K. Interaction of Carbohydrate Coated Cerium-Oxide Nanoparticles with Wheat and Pea: Stress Induction Potential and Effect on Development. Plants 2019, 8 (11). https://doi.org/10.3390/plants8110478" Plants (2019)

Effects of Ag+ ion doping on UV radiation absorption and luminescence profiles of fluorapatite nanomaterials obtained by neutralization method

Milojkov, Dušan; Stanić, Vojislav; Dimović, Slavko; Mutavdžić, Dragosav; Živković-Radovanović, Vukosava; Janjić, Goran V.; Radotić, Ksenija

(Polish Academy of Sciences, 2019)

TY  - JOUR
AU  - Milojkov, Dušan
AU  - Stanić, Vojislav
AU  - Dimović, Slavko
AU  - Mutavdžić, Dragosav
AU  - Živković-Radovanović, Vukosava
AU  - Janjić, Goran V.
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3717
AB  - In the present study we have analyzed effects of Ag+ ions doping on energetic profiles of nanophosphors materials based on fluorapatite crystal system. The UV radiation absorption and luminescence properties of monophase fluorapatite (FAP) and Ag+ doped fluorapatite (AgFAP) nanomaterials obtained by neutralization method were investigated using the photoluminescence spectrophotometry. The excitation-emission profiles of nanomaterials were analyzed statistically by MCR-ALS method and number of fluorophores was extracted. FAP lattice absorbed light at 350 nm in the UVA part of spectrum, and with increasing concentration of Ag+ ions new absorption maximum appeared at 270 nm in the UVC part. Fluorescence of FAP nanoparticles was in violet region of visible part of the spectrum, with a red shift to the green region when Ag+ was doped in lattice. MCR-ALS analyses of fluorescence spectra confirm formation of two maxima, at 484 and 505 nm, as a consequence of Ag+ ions doping in FAP lattice at Ca1 (4f) sites. The results of quantum chemical calculations showed that an Ag+ ion is stronger bonded to the binding site 1 (-1352:6 kcal/mol) than to the binding site 2 (-1249:0 kcal/mol). Considering that AgFAP1 nanopowder absorbs photons over all part of UV radiation spectrum, this material might be used as potential radiation protective nanomaterial.
PB  - Polish Academy of Sciences
T2  - Acta Physica Polonica A
T1  - Effects of Ag+ ion doping on UV radiation absorption and luminescence profiles of fluorapatite nanomaterials obtained by neutralization method
VL  - 136
IS  - 1
SP  - 86
EP  - 91
DO  - 10.12693/APhysPolA.136.86
ER  - 
@article{
author = "Milojkov, Dušan and Stanić, Vojislav and Dimović, Slavko and Mutavdžić, Dragosav and Živković-Radovanović, Vukosava and Janjić, Goran V. and Radotić, Ksenija",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3717",
abstract = "In the present study we have analyzed effects of Ag+ ions doping on energetic profiles of nanophosphors materials based on fluorapatite crystal system. The UV radiation absorption and luminescence properties of monophase fluorapatite (FAP) and Ag+ doped fluorapatite (AgFAP) nanomaterials obtained by neutralization method were investigated using the photoluminescence spectrophotometry. The excitation-emission profiles of nanomaterials were analyzed statistically by MCR-ALS method and number of fluorophores was extracted. FAP lattice absorbed light at 350 nm in the UVA part of spectrum, and with increasing concentration of Ag+ ions new absorption maximum appeared at 270 nm in the UVC part. Fluorescence of FAP nanoparticles was in violet region of visible part of the spectrum, with a red shift to the green region when Ag+ was doped in lattice. MCR-ALS analyses of fluorescence spectra confirm formation of two maxima, at 484 and 505 nm, as a consequence of Ag+ ions doping in FAP lattice at Ca1 (4f) sites. The results of quantum chemical calculations showed that an Ag+ ion is stronger bonded to the binding site 1 (-1352:6 kcal/mol) than to the binding site 2 (-1249:0 kcal/mol). Considering that AgFAP1 nanopowder absorbs photons over all part of UV radiation spectrum, this material might be used as potential radiation protective nanomaterial.",
publisher = "Polish Academy of Sciences",
journal = "Acta Physica Polonica A",
title = "Effects of Ag+ ion doping on UV radiation absorption and luminescence profiles of fluorapatite nanomaterials obtained by neutralization method",
volume = "136",
number = "1",
pages = "86-91",
doi = "10.12693/APhysPolA.136.86"
}
Milojkov, D., Stanić, V., Dimović, S., Mutavdžić, D., Živković-Radovanović, V., Janjić, G. V.,& Radotić, K. (2019). Effects of Ag+ ion doping on UV radiation absorption and luminescence profiles of fluorapatite nanomaterials obtained by neutralization method.
Acta Physica Polonica A
Polish Academy of Sciences., 136(1), 86-91.
https://doi.org/10.12693/APhysPolA.136.86
Milojkov D, Stanić V, Dimović S, Mutavdžić D, Živković-Radovanović V, Janjić GV, Radotić K. Effects of Ag+ ion doping on UV radiation absorption and luminescence profiles of fluorapatite nanomaterials obtained by neutralization method. Acta Physica Polonica A. 2019;136(1):86-91
Milojkov Dušan, Stanić Vojislav, Dimović Slavko, Mutavdžić Dragosav, Živković-Radovanović Vukosava, Janjić Goran V., Radotić Ksenija, "Effects of Ag+ ion doping on UV radiation absorption and luminescence profiles of fluorapatite nanomaterials obtained by neutralization method" Acta Physica Polonica A, 136, no. 1 (2019):86-91,
https://doi.org/10.12693/APhysPolA.136.86 .

Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis

Stanković, M.; Bartolić, D.; Šikoparija, Branko; Spasojević, Dragica; Mutavdžić, Dragosav; Natić, Maja; Radotić, Ksenija

(2019)

TY  - JOUR
AU  - Stanković, M.
AU  - Bartolić, D.
AU  - Šikoparija, Branko
AU  - Spasojević, Dragica
AU  - Mutavdžić, Dragosav
AU  - Natić, Maja
AU  - Radotić, Ksenija
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3115
AB  - It has been explored to what extent the ratio of the two main fluorophores in honey, originating from proteins and phenolic compounds, change between the honey extraction stage and packaging in the jars. Fluorescence spectroscopy combined with multivariate curve resolution–alternating least squares (MCR–ALS) was used to determine the ratio of the spectral components originating from phenol and proteins (Ph/Pr) fl , as a ratiometric indicator of variability in selected Lime tree (Tilia L.) honey samples. Spectrophotometric quantification of phenols and proteins in the honey samples was also performed and their ratio (Ph/Pr) sp was calculated. The values of the (Ph/Pr) fl ratio and honey protein content after packaging depended on the quality of homogenization before packaging in jars. Colorimetric and fluorometric results for phenols and proteins were in compliance. The examples are the values 3.34, 3.30, and 9, 3.14 for (Ph/Pr) fl and corresponding values 2.64, 2.18, and 12.75, 2.31 for (Ph/Pr) sp , in all pairs, the first value presenting the sample after extraction and the second value the sample after packaging in jars. Both methods show that in the former case there is no change in the phenol/protein ratio, and in the latter case the ratio decreased.
T2  - Journal of Applied Spectroscopy
T1  - Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis
VL  - 86
IS  - 2
SP  - 256
EP  - 263
DO  - 10.1007/s10812-019-00809-1
ER  - 
@article{
author = "Stanković, M. and Bartolić, D. and Šikoparija, Branko and Spasojević, Dragica and Mutavdžić, Dragosav and Natić, Maja and Radotić, Ksenija",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3115",
abstract = "It has been explored to what extent the ratio of the two main fluorophores in honey, originating from proteins and phenolic compounds, change between the honey extraction stage and packaging in the jars. Fluorescence spectroscopy combined with multivariate curve resolution–alternating least squares (MCR–ALS) was used to determine the ratio of the spectral components originating from phenol and proteins (Ph/Pr) fl , as a ratiometric indicator of variability in selected Lime tree (Tilia L.) honey samples. Spectrophotometric quantification of phenols and proteins in the honey samples was also performed and their ratio (Ph/Pr) sp was calculated. The values of the (Ph/Pr) fl ratio and honey protein content after packaging depended on the quality of homogenization before packaging in jars. Colorimetric and fluorometric results for phenols and proteins were in compliance. The examples are the values 3.34, 3.30, and 9, 3.14 for (Ph/Pr) fl and corresponding values 2.64, 2.18, and 12.75, 2.31 for (Ph/Pr) sp , in all pairs, the first value presenting the sample after extraction and the second value the sample after packaging in jars. Both methods show that in the former case there is no change in the phenol/protein ratio, and in the latter case the ratio decreased.",
journal = "Journal of Applied Spectroscopy",
title = "Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis",
volume = "86",
number = "2",
pages = "256-263",
doi = "10.1007/s10812-019-00809-1"
}
Stanković, M., Bartolić, D., Šikoparija, B., Spasojević, D., Mutavdžić, D., Natić, M.,& Radotić, K. (2019). Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis.
Journal of Applied Spectroscopy, 86(2), 256-263.
https://doi.org/10.1007/s10812-019-00809-1
Stanković M, Bartolić D, Šikoparija B, Spasojević D, Mutavdžić D, Natić M, Radotić K. Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis. Journal of Applied Spectroscopy. 2019;86(2):256-263
Stanković M., Bartolić D., Šikoparija Branko, Spasojević Dragica, Mutavdžić Dragosav, Natić Maja, Radotić Ksenija, "Variability Estimation of the Protein and Phenol Total Content in Honey Using Front Face Fluorescence Spectroscopy Coupled with MCR–ALS Analysis" Journal of Applied Spectroscopy, 86, no. 2 (2019):256-263,
https://doi.org/10.1007/s10812-019-00809-1 .
3
3
2

Phenolic profile of Morus alba L. (mulberry) barrel staves

Smailagić, Anita; Dabić-Zagorac, Dragana; Veljović, Sonja; Gašić, Uroš M.; Pergal, Marija V.; Stanković, Mira; Radotić, Ksenija; Natić, Maja

(Kraków : Wydawnictwo Instytutu Zrównoważonej Energetyki, 2018)

TY  - CONF
AU  - Smailagić, Anita
AU  - Dabić-Zagorac, Dragana
AU  - Veljović, Sonja
AU  - Gašić, Uroš M.
AU  - Pergal, Marija V.
AU  - Stanković, Mira
AU  - Radotić, Ksenija
AU  - Natić, Maja
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2842
AB  - One of the most important practices in the production of some alcoholic beverages is
ageing process. During that process, important changes occur, which results in improved
sensory characteristics. 
The phenolic fraction was considered to be one of the most important parameters for
evaluating quality in the choice of good alternative aging wood [3]. So far, mulberry
heartwood hasn’t been investigated too much in order to characterize polyphenols. Therefore, the aim of this
research was to investigate phenolic profile of industrially dried mulberry staves originating
from Serbia. The results showed that mulberry was abundant in oxyresveratrol, taxifolin, (-
)-catechin gallate, and phenolic acids, such as protocatechuic, p-hydroxybenzoic and ellagic
acid.  Characteristic emission spectrum of the wood sample showed the maximum at 420
nm, being blue shifted comparing with the maxima of most hard- and soft- wood species.
This may be addressed to a relatively low content of lignin in mulberry wood. The extract
showed a maximum characteristic for the major polyphenols of oxyresveratrol and coumarin
type.
PB  - Kraków : Wydawnictwo Instytutu Zrównoważonej Energetyki
C3  - AGH International Student Conference Knowledge, Technology and Society - Book of Abstracts
T1  - Phenolic profile of Morus alba L. (mulberry) barrel staves
SP  - 71
EP  - 71
ER  - 
@conference{
author = "Smailagić, Anita and Dabić-Zagorac, Dragana and Veljović, Sonja and Gašić, Uroš M. and Pergal, Marija V. and Stanković, Mira and Radotić, Ksenija and Natić, Maja",
year = "2018",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2842",
abstract = "One of the most important practices in the production of some alcoholic beverages is
ageing process. During that process, important changes occur, which results in improved
sensory characteristics. 
The phenolic fraction was considered to be one of the most important parameters for
evaluating quality in the choice of good alternative aging wood [3]. So far, mulberry
heartwood hasn’t been investigated too much in order to characterize polyphenols. Therefore, the aim of this
research was to investigate phenolic profile of industrially dried mulberry staves originating
from Serbia. The results showed that mulberry was abundant in oxyresveratrol, taxifolin, (-
)-catechin gallate, and phenolic acids, such as protocatechuic, p-hydroxybenzoic and ellagic
acid.  Characteristic emission spectrum of the wood sample showed the maximum at 420
nm, being blue shifted comparing with the maxima of most hard- and soft- wood species.
This may be addressed to a relatively low content of lignin in mulberry wood. The extract
showed a maximum characteristic for the major polyphenols of oxyresveratrol and coumarin
type.",
publisher = "Kraków : Wydawnictwo Instytutu Zrównoważonej Energetyki",
journal = "AGH International Student Conference Knowledge, Technology and Society - Book of Abstracts",
title = "Phenolic profile of Morus alba L. (mulberry) barrel staves",
pages = "71-71"
}
Smailagić, A., Dabić-Zagorac, D., Veljović, S., Gašić, U. M., Pergal, M. V., Stanković, M., Radotić, K.,& Natić, M. (2018). Phenolic profile of Morus alba L. (mulberry) barrel staves.
AGH International Student Conference Knowledge, Technology and Society - Book of Abstracts
Kraków : Wydawnictwo Instytutu Zrównoważonej Energetyki., 71-71.
Smailagić A, Dabić-Zagorac D, Veljović S, Gašić UM, Pergal MV, Stanković M, Radotić K, Natić M. Phenolic profile of Morus alba L. (mulberry) barrel staves. AGH International Student Conference Knowledge, Technology and Society - Book of Abstracts. 2018;:71-71
Smailagić Anita, Dabić-Zagorac Dragana, Veljović Sonja, Gašić Uroš M., Pergal Marija V., Stanković Mira, Radotić Ksenija, Natić Maja, "Phenolic profile of Morus alba L. (mulberry) barrel staves" AGH International Student Conference Knowledge, Technology and Society - Book of Abstracts (2018):71-71

Supplementary material for the article: Milenković, I.; Radotić, K.; Matović, B.; Prekajski, M.; Živković, L.; Jakovljević, D.; Gojgić-Cvijović, G.; Beškoski, V. Improving Stability of Cerium Oxide Nanoparticles by Microbial Polysaccharides Coating. Journal of the Serbian Chemical Society 2018, 83 (6), 745–757. https://doi.org/10.2298/JSC171205031M

Milenković, Ivana; Radotić, Ksenija; Matović, Branko; Prekajski, Marija; Živković, Ljiljana S.; Jakovljević, Dragica M.; Gojgić-Cvijović, Gordana D.; Beškoski, Vladimir

(Serbian Chemical Soc, Belgrade, 2018)

TY  - BOOK
AU  - Milenković, Ivana
AU  - Radotić, Ksenija
AU  - Matović, Branko
AU  - Prekajski, Marija
AU  - Živković, Ljiljana S.
AU  - Jakovljević, Dragica M.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Beškoski, Vladimir
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3207
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Supplementary material for the article: Milenković, I.; Radotić, K.; Matović, B.; Prekajski, M.; Živković, L.; Jakovljević, D.; Gojgić-Cvijović, G.; Beškoski, V. Improving Stability of Cerium Oxide Nanoparticles by Microbial Polysaccharides Coating. Journal of the Serbian Chemical Society 2018, 83 (6), 745–757. https://doi.org/10.2298/JSC171205031M
ER  - 
@book{
author = "Milenković, Ivana and Radotić, Ksenija and Matović, Branko and Prekajski, Marija and Živković, Ljiljana S. and Jakovljević, Dragica M. and Gojgić-Cvijović, Gordana D. and Beškoski, Vladimir",
year = "2018",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3207",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Supplementary material for the article: Milenković, I.; Radotić, K.; Matović, B.; Prekajski, M.; Živković, L.; Jakovljević, D.; Gojgić-Cvijović, G.; Beškoski, V. Improving Stability of Cerium Oxide Nanoparticles by Microbial Polysaccharides Coating. Journal of the Serbian Chemical Society 2018, 83 (6), 745–757. https://doi.org/10.2298/JSC171205031M"
}
Milenković, I., Radotić, K., Matović, B., Prekajski, M., Živković, L. S., Jakovljević, D. M., Gojgić-Cvijović, G. D.,& Beškoski, V. (2018). Supplementary material for the article: Milenković, I.; Radotić, K.; Matović, B.; Prekajski, M.; Živković, L.; Jakovljević, D.; Gojgić-Cvijović, G.; Beškoski, V. Improving Stability of Cerium Oxide Nanoparticles by Microbial Polysaccharides Coating. Journal of the Serbian Chemical Society 2018, 83 (6), 745–757. https://doi.org/10.2298/JSC171205031M.
Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade..
Milenković I, Radotić K, Matović B, Prekajski M, Živković LS, Jakovljević DM, Gojgić-Cvijović GD, Beškoski V. Supplementary material for the article: Milenković, I.; Radotić, K.; Matović, B.; Prekajski, M.; Živković, L.; Jakovljević, D.; Gojgić-Cvijović, G.; Beškoski, V. Improving Stability of Cerium Oxide Nanoparticles by Microbial Polysaccharides Coating. Journal of the Serbian Chemical Society 2018, 83 (6), 745–757. https://doi.org/10.2298/JSC171205031M. Journal of the Serbian Chemical Society. 2018;
Milenković Ivana, Radotić Ksenija, Matović Branko, Prekajski Marija, Živković Ljiljana S., Jakovljević Dragica M., Gojgić-Cvijović Gordana D., Beškoski Vladimir, "Supplementary material for the article: Milenković, I.; Radotić, K.; Matović, B.; Prekajski, M.; Živković, L.; Jakovljević, D.; Gojgić-Cvijović, G.; Beškoski, V. Improving Stability of Cerium Oxide Nanoparticles by Microbial Polysaccharides Coating. Journal of the Serbian Chemical Society 2018, 83 (6), 745–757. https://doi.org/10.2298/JSC171205031M" Journal of the Serbian Chemical Society (2018)

Improving stability of cerium oxide nanoparticles by microbial polysaccharides coating

Milenković, Ivana; Radotić, Ksenija; Matović, Branko; Prekajski, Marija; Živković, Ljiljana S.; Jakovljević, Dragica M.; Gojgić-Cvijović, Gordana D.; Beškoski, Vladimir

(Serbian Chemical Soc, Belgrade, 2018)

TY  - JOUR
AU  - Milenković, Ivana
AU  - Radotić, Ksenija
AU  - Matović, Branko
AU  - Prekajski, Marija
AU  - Živković, Ljiljana S.
AU  - Jakovljević, Dragica M.
AU  - Gojgić-Cvijović, Gordana D.
AU  - Beškoski, Vladimir
PY  - 2018
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2087
AB  - Cerium oxide (CeO2) nanoparticles (CONPs) are interesting biomaterials with various applications in biomedicine, cosmetics and the pharmaceutical industry, but with limited practical application because of their low stability in aqueous media. The aim of this study was to obtain CONPs with increased stability by coating the particles. Microbial exopolysaccharides (levan, pullulan) and glucose were used to prepare CONPs under different synthesis conditions. Coating was attempted by adding the carbohydrates during (direct coating) or after (subsequent coating) the synthesis of CONPs. The obtained nanoparticles were characterized by X-Ray diffraction analysis, Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The suspension stability of the uncoated and coated CONPs in aqueous media was evaluated by measuring the hydrodynamic size, zeta potential and turbidity. The FT-IR spectra revealed the differences between coated CONPs and showed the success of subsequent coating with carbohydrates. Coating with carbohydrates improved the stability the CONP suspension by decreasing the size of aggregated particles. The suspensions of levan- and glucose-coated CONPs had the best stability. In this study, CONPs were prepared using non-toxic materials, which were completely environmentally friendly. The obtained results open new horizons for CONP synthesis, improving their biological applications.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Improving stability of cerium oxide nanoparticles by microbial polysaccharides coating
VL  - 83
IS  - 6
SP  - 745
EP  - 757
DO  - 10.2298/JSC171205031M
ER  - 
@article{
author = "Milenković, Ivana and Radotić, Ksenija and Matović, Branko and Prekajski, Marija and Živković, Ljiljana S. and Jakovljević, Dragica M. and Gojgić-Cvijović, Gordana D. and Beškoski, Vladimir",
year = "2018",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2087",
abstract = "Cerium oxide (CeO2) nanoparticles (CONPs) are interesting biomaterials with various applications in biomedicine, cosmetics and the pharmaceutical industry, but with limited practical application because of their low stability in aqueous media. The aim of this study was to obtain CONPs with increased stability by coating the particles. Microbial exopolysaccharides (levan, pullulan) and glucose were used to prepare CONPs under different synthesis conditions. Coating was attempted by adding the carbohydrates during (direct coating) or after (subsequent coating) the synthesis of CONPs. The obtained nanoparticles were characterized by X-Ray diffraction analysis, Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The suspension stability of the uncoated and coated CONPs in aqueous media was evaluated by measuring the hydrodynamic size, zeta potential and turbidity. The FT-IR spectra revealed the differences between coated CONPs and showed the success of subsequent coating with carbohydrates. Coating with carbohydrates improved the stability the CONP suspension by decreasing the size of aggregated particles. The suspensions of levan- and glucose-coated CONPs had the best stability. In this study, CONPs were prepared using non-toxic materials, which were completely environmentally friendly. The obtained results open new horizons for CONP synthesis, improving their biological applications.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Improving stability of cerium oxide nanoparticles by microbial polysaccharides coating",
volume = "83",
number = "6",
pages = "745-757",
doi = "10.2298/JSC171205031M"
}
Milenković, I., Radotić, K., Matović, B., Prekajski, M., Živković, L. S., Jakovljević, D. M., Gojgić-Cvijović, G. D.,& Beškoski, V. (2018). Improving stability of cerium oxide nanoparticles by microbial polysaccharides coating.
Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 83(6), 745-757.
https://doi.org/10.2298/JSC171205031M
Milenković I, Radotić K, Matović B, Prekajski M, Živković LS, Jakovljević DM, Gojgić-Cvijović GD, Beškoski V. Improving stability of cerium oxide nanoparticles by microbial polysaccharides coating. Journal of the Serbian Chemical Society. 2018;83(6):745-757
Milenković Ivana, Radotić Ksenija, Matović Branko, Prekajski Marija, Živković Ljiljana S., Jakovljević Dragica M., Gojgić-Cvijović Gordana D., Beškoski Vladimir, "Improving stability of cerium oxide nanoparticles by microbial polysaccharides coating" Journal of the Serbian Chemical Society, 83, no. 6 (2018):745-757,
https://doi.org/10.2298/JSC171205031M .
9
6
9

Supplementary data for article: Prokopijevic, M.; Prodanovic, O.; Spasojevic, D.; Kovacevic, G.; Polovic, N.; Radotic, K.; Prodanovic, R. Tyramine-Modified Pectins via Periodate Oxidation for Soybean Hull Peroxidase Induced Hydrogel Formation and Immobilization. Applied Microbiology and Biotechnology 2017, 101 (6), 2281–2290. https://doi.org/10.1007/s00253-016-8002-x

Prokopijević, Miloš; Prodanović, Olivera; Spasojević, Dragica; Kovačević, Gordana; Polović, Natalija; Radotić, Ksenija; Prodanović, Radivoje

(Springer, New York, 2017)

TY  - BOOK
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Spasojević, Dragica
AU  - Kovačević, Gordana
AU  - Polović, Natalija
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2017
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3053
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Supplementary data for article: Prokopijevic, M.; Prodanovic, O.; Spasojevic, D.; Kovacevic, G.; Polovic, N.; Radotic, K.; Prodanovic, R. Tyramine-Modified Pectins via Periodate Oxidation for Soybean Hull Peroxidase Induced Hydrogel Formation and Immobilization. Applied Microbiology and Biotechnology 2017, 101 (6), 2281–2290. https://doi.org/10.1007/s00253-016-8002-x
ER  - 
@book{
author = "Prokopijević, Miloš and Prodanović, Olivera and Spasojević, Dragica and Kovačević, Gordana and Polović, Natalija and Radotić, Ksenija and Prodanović, Radivoje",
year = "2017",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3053",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Supplementary data for article: Prokopijevic, M.; Prodanovic, O.; Spasojevic, D.; Kovacevic, G.; Polovic, N.; Radotic, K.; Prodanovic, R. Tyramine-Modified Pectins via Periodate Oxidation for Soybean Hull Peroxidase Induced Hydrogel Formation and Immobilization. Applied Microbiology and Biotechnology 2017, 101 (6), 2281–2290. https://doi.org/10.1007/s00253-016-8002-x"
}
Prokopijević, M., Prodanović, O., Spasojević, D., Kovačević, G., Polović, N., Radotić, K.,& Prodanović, R. (2017). Supplementary data for article: Prokopijevic, M.; Prodanovic, O.; Spasojevic, D.; Kovacevic, G.; Polovic, N.; Radotic, K.; Prodanovic, R. Tyramine-Modified Pectins via Periodate Oxidation for Soybean Hull Peroxidase Induced Hydrogel Formation and Immobilization. Applied Microbiology and Biotechnology 2017, 101 (6), 2281–2290. https://doi.org/10.1007/s00253-016-8002-x.
Applied Microbiology and Biotechnology
Springer, New York..
Prokopijević M, Prodanović O, Spasojević D, Kovačević G, Polović N, Radotić K, Prodanović R. Supplementary data for article: Prokopijevic, M.; Prodanovic, O.; Spasojevic, D.; Kovacevic, G.; Polovic, N.; Radotic, K.; Prodanovic, R. Tyramine-Modified Pectins via Periodate Oxidation for Soybean Hull Peroxidase Induced Hydrogel Formation and Immobilization. Applied Microbiology and Biotechnology 2017, 101 (6), 2281–2290. https://doi.org/10.1007/s00253-016-8002-x. Applied Microbiology and Biotechnology. 2017;
Prokopijević Miloš, Prodanović Olivera, Spasojević Dragica, Kovačević Gordana, Polović Natalija, Radotić Ksenija, Prodanović Radivoje, "Supplementary data for article: Prokopijevic, M.; Prodanovic, O.; Spasojevic, D.; Kovacevic, G.; Polovic, N.; Radotic, K.; Prodanovic, R. Tyramine-Modified Pectins via Periodate Oxidation for Soybean Hull Peroxidase Induced Hydrogel Formation and Immobilization. Applied Microbiology and Biotechnology 2017, 101 (6), 2281–2290. https://doi.org/10.1007/s00253-016-8002-x" Applied Microbiology and Biotechnology (2017)

Tyramine-modified pectins via periodate oxidation for soybean hull peroxidase induced hydrogel formation and immobilization

Prokopijević, Miloš; Prodanović, Olivera; Spasojević, Dragica; Kovačević, Gordana; Polović, Natalija; Radotić, Ksenija; Prodanović, Radivoje

(Springer, New York, 2017)

TY  - JOUR
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Spasojević, Dragica
AU  - Kovačević, Gordana
AU  - Polović, Natalija
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2017
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2421
AB  - Pectin was modified by oxidation with sodium periodate at molar ratios of 2.5, 5, 10, 15 and 20 mol% and reductive amination with tyramine and sodium cyanoborohydride afterwards. Concentration of tyramine groups within modified pectin ranged from 54.5 to 538 mu mol/g of dry pectin while concentration of ionizable groups ranged from 3.0 to 4.0 mmol/g of dry polymer compared to 1.5 mmol/g before modification due to the introduction of amino group. All tyramine-pectins showed exceptional gelling properties and could form hydrogel both by cross-linking of carboxyl groups with calcium or by cross-linking phenol groups with peroxidase in the presence of hydrogen peroxide. These hydrogels were tested as carriers for soybean hull peroxidase (SHP) immobilization within microbeads formed in an emulsion based enzymatic polymerization reaction. SHP immobilized within tyramine-pectin microbeads had an increased thermal and organic solvent stability compared to the soluble enzyme. Immobilized SHP was more active in acidic pH region and had slightly decreased K (m) value of 2.61 mM compared to the soluble enzyme. After 7 cycles of repeated use in batch reactor for pyrogallol oxidation microbeads, immobilized SHP retained half of the initial activity.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Tyramine-modified pectins via periodate oxidation for soybean hull peroxidase induced hydrogel formation and immobilization
VL  - 101
IS  - 6
SP  - 2281
EP  - 2290
DO  - 10.1007/s00253-016-8002-x
ER  - 
@article{
author = "Prokopijević, Miloš and Prodanović, Olivera and Spasojević, Dragica and Kovačević, Gordana and Polović, Natalija and Radotić, Ksenija and Prodanović, Radivoje",
year = "2017",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2421",
abstract = "Pectin was modified by oxidation with sodium periodate at molar ratios of 2.5, 5, 10, 15 and 20 mol% and reductive amination with tyramine and sodium cyanoborohydride afterwards. Concentration of tyramine groups within modified pectin ranged from 54.5 to 538 mu mol/g of dry pectin while concentration of ionizable groups ranged from 3.0 to 4.0 mmol/g of dry polymer compared to 1.5 mmol/g before modification due to the introduction of amino group. All tyramine-pectins showed exceptional gelling properties and could form hydrogel both by cross-linking of carboxyl groups with calcium or by cross-linking phenol groups with peroxidase in the presence of hydrogen peroxide. These hydrogels were tested as carriers for soybean hull peroxidase (SHP) immobilization within microbeads formed in an emulsion based enzymatic polymerization reaction. SHP immobilized within tyramine-pectin microbeads had an increased thermal and organic solvent stability compared to the soluble enzyme. Immobilized SHP was more active in acidic pH region and had slightly decreased K (m) value of 2.61 mM compared to the soluble enzyme. After 7 cycles of repeated use in batch reactor for pyrogallol oxidation microbeads, immobilized SHP retained half of the initial activity.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Tyramine-modified pectins via periodate oxidation for soybean hull peroxidase induced hydrogel formation and immobilization",
volume = "101",
number = "6",
pages = "2281-2290",
doi = "10.1007/s00253-016-8002-x"
}
Prokopijević, M., Prodanović, O., Spasojević, D., Kovačević, G., Polović, N., Radotić, K.,& Prodanović, R. (2017). Tyramine-modified pectins via periodate oxidation for soybean hull peroxidase induced hydrogel formation and immobilization.
Applied Microbiology and Biotechnology
Springer, New York., 101(6), 2281-2290.
https://doi.org/10.1007/s00253-016-8002-x
Prokopijević M, Prodanović O, Spasojević D, Kovačević G, Polović N, Radotić K, Prodanović R. Tyramine-modified pectins via periodate oxidation for soybean hull peroxidase induced hydrogel formation and immobilization. Applied Microbiology and Biotechnology. 2017;101(6):2281-2290
Prokopijević Miloš, Prodanović Olivera, Spasojević Dragica, Kovačević Gordana, Polović Natalija, Radotić Ksenija, Prodanović Radivoje, "Tyramine-modified pectins via periodate oxidation for soybean hull peroxidase induced hydrogel formation and immobilization" Applied Microbiology and Biotechnology, 101, no. 6 (2017):2281-2290,
https://doi.org/10.1007/s00253-016-8002-x .
8
5
7

Supplementary data for the article: Spasojević, D.; Zmejkoski, D.; Glamočlija, J.; Nikolić, M.; Soković, M.; Milošević, V.; Jarić, I.; Stojanović, M.; Marinković, E.; Barisani-Asenbauer, T.; et al. Lignin Model Compound in Alginate Hydrogel: A Strong Antimicrobial Agent with High Potential in Wound Treatment. International Journal of Antimicrobial Agents 2016, 48 (6), 732–735. https://doi.org/10.1016/j.ijantimicag.2016.08.014

Spasojević, Dragica; Zmejkoski, Danica; Glamoclija, Jasmina; Nikolić, Miloš; Soković, Marina; Milošević, Verica; Jarić, Ivana; Stojanović, Marijana M.; Marinković, Emilija; Barisani-Asenbauer, Talin; Prodanović, Radivoje; Jovanović, Miloš; Radotić, Ksenija

(Elsevier Science Bv, Amsterdam, 2016)

TY  - BOOK
AU  - Spasojević, Dragica
AU  - Zmejkoski, Danica
AU  - Glamoclija, Jasmina
AU  - Nikolić, Miloš
AU  - Soković, Marina
AU  - Milošević, Verica
AU  - Jarić, Ivana
AU  - Stojanović, Marijana M.
AU  - Marinković, Emilija
AU  - Barisani-Asenbauer, Talin
AU  - Prodanović, Radivoje
AU  - Jovanović, Miloš
AU  - Radotić, Ksenija
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3560
PB  - Elsevier Science Bv, Amsterdam
T2  - International Journal of Antimicrobial Agents
T1  - Supplementary data for the article: Spasojević, D.; Zmejkoski, D.; Glamočlija, J.; Nikolić, M.; Soković, M.; Milošević, V.; Jarić, I.; Stojanović, M.; Marinković, E.; Barisani-Asenbauer, T.; et al. Lignin Model Compound in Alginate Hydrogel: A Strong Antimicrobial Agent with High Potential in Wound Treatment. International Journal of Antimicrobial Agents 2016, 48 (6), 732–735. https://doi.org/10.1016/j.ijantimicag.2016.08.014
ER  - 
@book{
author = "Spasojević, Dragica and Zmejkoski, Danica and Glamoclija, Jasmina and Nikolić, Miloš and Soković, Marina and Milošević, Verica and Jarić, Ivana and Stojanović, Marijana M. and Marinković, Emilija and Barisani-Asenbauer, Talin and Prodanović, Radivoje and Jovanović, Miloš and Radotić, Ksenija",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3560",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Journal of Antimicrobial Agents",
title = "Supplementary data for the article: Spasojević, D.; Zmejkoski, D.; Glamočlija, J.; Nikolić, M.; Soković, M.; Milošević, V.; Jarić, I.; Stojanović, M.; Marinković, E.; Barisani-Asenbauer, T.; et al. Lignin Model Compound in Alginate Hydrogel: A Strong Antimicrobial Agent with High Potential in Wound Treatment. International Journal of Antimicrobial Agents 2016, 48 (6), 732–735. https://doi.org/10.1016/j.ijantimicag.2016.08.014"
}
Spasojević, D., Zmejkoski, D., Glamoclija, J., Nikolić, M., Soković, M., Milošević, V., Jarić, I., Stojanović, M. M., Marinković, E., Barisani-Asenbauer, T., Prodanović, R., Jovanović, M.,& Radotić, K. (2016). Supplementary data for the article: Spasojević, D.; Zmejkoski, D.; Glamočlija, J.; Nikolić, M.; Soković, M.; Milošević, V.; Jarić, I.; Stojanović, M.; Marinković, E.; Barisani-Asenbauer, T.; et al. Lignin Model Compound in Alginate Hydrogel: A Strong Antimicrobial Agent with High Potential in Wound Treatment. International Journal of Antimicrobial Agents 2016, 48 (6), 732–735. https://doi.org/10.1016/j.ijantimicag.2016.08.014.
International Journal of Antimicrobial Agents
Elsevier Science Bv, Amsterdam..
Spasojević D, Zmejkoski D, Glamoclija J, Nikolić M, Soković M, Milošević V, Jarić I, Stojanović MM, Marinković E, Barisani-Asenbauer T, Prodanović R, Jovanović M, Radotić K. Supplementary data for the article: Spasojević, D.; Zmejkoski, D.; Glamočlija, J.; Nikolić, M.; Soković, M.; Milošević, V.; Jarić, I.; Stojanović, M.; Marinković, E.; Barisani-Asenbauer, T.; et al. Lignin Model Compound in Alginate Hydrogel: A Strong Antimicrobial Agent with High Potential in Wound Treatment. International Journal of Antimicrobial Agents 2016, 48 (6), 732–735. https://doi.org/10.1016/j.ijantimicag.2016.08.014. International Journal of Antimicrobial Agents. 2016;
Spasojević Dragica, Zmejkoski Danica, Glamoclija Jasmina, Nikolić Miloš, Soković Marina, Milošević Verica, Jarić Ivana, Stojanović Marijana M., Marinković Emilija, Barisani-Asenbauer Talin, Prodanović Radivoje, Jovanović Miloš, Radotić Ksenija, "Supplementary data for the article: Spasojević, D.; Zmejkoski, D.; Glamočlija, J.; Nikolić, M.; Soković, M.; Milošević, V.; Jarić, I.; Stojanović, M.; Marinković, E.; Barisani-Asenbauer, T.; et al. Lignin Model Compound in Alginate Hydrogel: A Strong Antimicrobial Agent with High Potential in Wound Treatment. International Journal of Antimicrobial Agents 2016, 48 (6), 732–735. https://doi.org/10.1016/j.ijantimicag.2016.08.014" International Journal of Antimicrobial Agents (2016)

Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment

Spasojević, Dragica; Zmejkoski, Danica; Glamoclija, Jasmina; Nikolić, Miloš; Soković, Marina; Milošević, Verica; Jarić, Ivana; Stojanović, Marijana M.; Marinković, Emilija; Barisani-Asenbauer, Talin; Prodanović, Radivoje; Jovanović, Miloš; Radotić, Ksenija

(Elsevier Science Bv, Amsterdam, 2016)

TY  - JOUR
AU  - Spasojević, Dragica
AU  - Zmejkoski, Danica
AU  - Glamoclija, Jasmina
AU  - Nikolić, Miloš
AU  - Soković, Marina
AU  - Milošević, Verica
AU  - Jarić, Ivana
AU  - Stojanović, Marijana M.
AU  - Marinković, Emilija
AU  - Barisani-Asenbauer, Talin
AU  - Prodanović, Radivoje
AU  - Jovanović, Miloš
AU  - Radotić, Ksenija
PY  - 2016
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/2364
AB  - Nowadays bacterial resistance to known antibiotics is a serious health problem. In order to achieve more efficient treatment, lately there is an effort to find new substances, such as certain biomaterials, that are non-toxic to humans with antibiotic potential. Lignins and lignin-derived compounds have been proposed to be good candidates for use in medicine and health maintenance. In this study, the antibacterial activity of the lignin model polymer dehydrogenate polymer (DHP) in alginate hydrogel (Alg) was studied. The obtained results show that DHP-Alg has strong antimicrobial activity against several bacterial strains and biofilms and does not have a toxic effect on human epithelial cells. These results strongly suggest its application as a wound healing agent or as an adjunct substance for wound treatments. (C) 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - International Journal of Antimicrobial Agents
T1  - Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment
VL  - 48
IS  - 6
SP  - 732
EP  - 735
DO  - 10.1016/j.ijantimicag.2016.08.014
ER  - 
@article{
author = "Spasojević, Dragica and Zmejkoski, Danica and Glamoclija, Jasmina and Nikolić, Miloš and Soković, Marina and Milošević, Verica and Jarić, Ivana and Stojanović, Marijana M. and Marinković, Emilija and Barisani-Asenbauer, Talin and Prodanović, Radivoje and Jovanović, Miloš and Radotić, Ksenija",
year = "2016",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/2364",
abstract = "Nowadays bacterial resistance to known antibiotics is a serious health problem. In order to achieve more efficient treatment, lately there is an effort to find new substances, such as certain biomaterials, that are non-toxic to humans with antibiotic potential. Lignins and lignin-derived compounds have been proposed to be good candidates for use in medicine and health maintenance. In this study, the antibacterial activity of the lignin model polymer dehydrogenate polymer (DHP) in alginate hydrogel (Alg) was studied. The obtained results show that DHP-Alg has strong antimicrobial activity against several bacterial strains and biofilms and does not have a toxic effect on human epithelial cells. These results strongly suggest its application as a wound healing agent or as an adjunct substance for wound treatments. (C) 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Journal of Antimicrobial Agents",
title = "Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment",
volume = "48",
number = "6",
pages = "732-735",
doi = "10.1016/j.ijantimicag.2016.08.014"
}
Spasojević, D., Zmejkoski, D., Glamoclija, J., Nikolić, M., Soković, M., Milošević, V., Jarić, I., Stojanović, M. M., Marinković, E., Barisani-Asenbauer, T., Prodanović, R., Jovanović, M.,& Radotić, K. (2016). Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment.
International Journal of Antimicrobial Agents
Elsevier Science Bv, Amsterdam., 48(6), 732-735.
https://doi.org/10.1016/j.ijantimicag.2016.08.014
Spasojević D, Zmejkoski D, Glamoclija J, Nikolić M, Soković M, Milošević V, Jarić I, Stojanović MM, Marinković E, Barisani-Asenbauer T, Prodanović R, Jovanović M, Radotić K. Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment. International Journal of Antimicrobial Agents. 2016;48(6):732-735
Spasojević Dragica, Zmejkoski Danica, Glamoclija Jasmina, Nikolić Miloš, Soković Marina, Milošević Verica, Jarić Ivana, Stojanović Marijana M., Marinković Emilija, Barisani-Asenbauer Talin, Prodanović Radivoje, Jovanović Miloš, Radotić Ksenija, "Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment" International Journal of Antimicrobial Agents, 48, no. 6 (2016):732-735,
https://doi.org/10.1016/j.ijantimicag.2016.08.014 .
22
17
18

Supplementary data for article: Prodanović, O.; Spasojević, D.; Prokopijević, M.; Radotić, K.; Markovic, N.; Blažić, M.; Prodanović, R. Tyramine Modified Alginates via Periodate Oxidation for Peroxidase Induced Hydrogel Formation and Immobilization. Reactive and Functional Polymers 2015, 93, 77–83. https://doi.org/10.1016/j.reactfunctpolym.2015.06.004

Prodanović, Olivera; Spasojević, Dragica; Prokopijević, Miloš; Radotić, Ksenija; Marković, Nevena; Blažić, Marija; Prodanović, Radivoje

(Elsevier Science Bv, Amsterdam, 2015)

TY  - BOOK
AU  - Prodanović, Olivera
AU  - Spasojević, Dragica
AU  - Prokopijević, Miloš
AU  - Radotić, Ksenija
AU  - Marković, Nevena
AU  - Blažić, Marija
AU  - Prodanović, Radivoje
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3457
PB  - Elsevier Science Bv, Amsterdam
T2  - Reactive and Functional Polymers
T1  - Supplementary data for article: Prodanović, O.; Spasojević, D.; Prokopijević, M.; Radotić, K.; Markovic, N.; Blažić, M.; Prodanović, R. Tyramine Modified Alginates via Periodate Oxidation for Peroxidase Induced Hydrogel Formation and Immobilization. Reactive and Functional Polymers 2015, 93, 77–83. https://doi.org/10.1016/j.reactfunctpolym.2015.06.004
ER  - 
@book{
author = "Prodanović, Olivera and Spasojević, Dragica and Prokopijević, Miloš and Radotić, Ksenija and Marković, Nevena and Blažić, Marija and Prodanović, Radivoje",
year = "2015",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3457",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Reactive and Functional Polymers",
title = "Supplementary data for article: Prodanović, O.; Spasojević, D.; Prokopijević, M.; Radotić, K.; Markovic, N.; Blažić, M.; Prodanović, R. Tyramine Modified Alginates via Periodate Oxidation for Peroxidase Induced Hydrogel Formation and Immobilization. Reactive and Functional Polymers 2015, 93, 77–83. https://doi.org/10.1016/j.reactfunctpolym.2015.06.004"
}
Prodanović, O., Spasojević, D., Prokopijević, M., Radotić, K., Marković, N., Blažić, M.,& Prodanović, R. (2015). Supplementary data for article: Prodanović, O.; Spasojević, D.; Prokopijević, M.; Radotić, K.; Markovic, N.; Blažić, M.; Prodanović, R. Tyramine Modified Alginates via Periodate Oxidation for Peroxidase Induced Hydrogel Formation and Immobilization. Reactive and Functional Polymers 2015, 93, 77–83. https://doi.org/10.1016/j.reactfunctpolym.2015.06.004.
Reactive and Functional Polymers
Elsevier Science Bv, Amsterdam..
Prodanović O, Spasojević D, Prokopijević M, Radotić K, Marković N, Blažić M, Prodanović R. Supplementary data for article: Prodanović, O.; Spasojević, D.; Prokopijević, M.; Radotić, K.; Markovic, N.; Blažić, M.; Prodanović, R. Tyramine Modified Alginates via Periodate Oxidation for Peroxidase Induced Hydrogel Formation and Immobilization. Reactive and Functional Polymers 2015, 93, 77–83. https://doi.org/10.1016/j.reactfunctpolym.2015.06.004. Reactive and Functional Polymers. 2015;
Prodanović Olivera, Spasojević Dragica, Prokopijević Miloš, Radotić Ksenija, Marković Nevena, Blažić Marija, Prodanović Radivoje, "Supplementary data for article: Prodanović, O.; Spasojević, D.; Prokopijević, M.; Radotić, K.; Markovic, N.; Blažić, M.; Prodanović, R. Tyramine Modified Alginates via Periodate Oxidation for Peroxidase Induced Hydrogel Formation and Immobilization. Reactive and Functional Polymers 2015, 93, 77–83. https://doi.org/10.1016/j.reactfunctpolym.2015.06.004" Reactive and Functional Polymers (2015)

Tyramine modified alginates via periodate oxidation for peroxidase induced hydrogel formation and immobilization

Prodanović, Olivera; Spasojević, Dragica; Prokopijević, Miloš; Radotić, Ksenija; Marković, Nevena; Blažić, Marija; Prodanović, Radivoje

(Elsevier Science Bv, Amsterdam, 2015)

TY  - JOUR
AU  - Prodanović, Olivera
AU  - Spasojević, Dragica
AU  - Prokopijević, Miloš
AU  - Radotić, Ksenija
AU  - Marković, Nevena
AU  - Blažić, Marija
AU  - Prodanović, Radivoje
PY  - 2015
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1966
AB  - Phenol and amino groups were introduced into alginate to different degrees via oxidation with 2.5, 5, 10, 15 and 20 mol% of periodate and reductive amination by tyramine. Modification of alginate with tyramine was confirmed by FTIR spectroscopy and UV-VIS spectroscopy, while concentration of phenol and ionizable groups was determined using absorbance at 275 nm and acid-base titration. All tyramine-alginates were able to form hydrogels after cross-linking with horse radish peroxidase (HRP) and hydrogen peroxide. Tyramine-alginates oxidized with up to 10 mol% of periodate were also capable of forming hydrogels with calcium ions. Tyramine-alginates were tested for HRP immobilization within micro-beads obtained by peroxidase catalyzed droplet polymerization using internal delivery of hydrogen peroxide via glucose oxidase and glucose. Highest activity of immobilized peroxidase was obtained with 20% (w/v) tyramine-alginate obtained via 20 mol% periodate oxidation. Immobilized enzyme was not leaking from the micro-beads and was further kinetically characterized for pyrogallol oxidation. Km for pyrogallol was increased after immobilization from 1.93 mM for soluble HRP to 734 mM for immobilized HRP. The optimum pH was also increased from pH 7.0 to 8.0. Temperature and organic solvent stability improved significantly after immobilization, so that half-life at 70 degrees C increased around four times, while half-life in 80% (v/v) dioxane increased 22 times. After repeated use of 6 times in batch reactor for pyrogallol oxidation immobilized HRP retained 45% of original activity.
PB  - Elsevier Science Bv, Amsterdam
T2  - Reactive and Functional Polymers
T1  - Tyramine modified alginates via periodate oxidation for peroxidase induced hydrogel formation and immobilization
VL  - 93
SP  - 77
EP  - 83
DO  - 10.1016/j.reactfunctpolym.2015.06.004
ER  - 
@article{
author = "Prodanović, Olivera and Spasojević, Dragica and Prokopijević, Miloš and Radotić, Ksenija and Marković, Nevena and Blažić, Marija and Prodanović, Radivoje",
year = "2015",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1966",
abstract = "Phenol and amino groups were introduced into alginate to different degrees via oxidation with 2.5, 5, 10, 15 and 20 mol% of periodate and reductive amination by tyramine. Modification of alginate with tyramine was confirmed by FTIR spectroscopy and UV-VIS spectroscopy, while concentration of phenol and ionizable groups was determined using absorbance at 275 nm and acid-base titration. All tyramine-alginates were able to form hydrogels after cross-linking with horse radish peroxidase (HRP) and hydrogen peroxide. Tyramine-alginates oxidized with up to 10 mol% of periodate were also capable of forming hydrogels with calcium ions. Tyramine-alginates were tested for HRP immobilization within micro-beads obtained by peroxidase catalyzed droplet polymerization using internal delivery of hydrogen peroxide via glucose oxidase and glucose. Highest activity of immobilized peroxidase was obtained with 20% (w/v) tyramine-alginate obtained via 20 mol% periodate oxidation. Immobilized enzyme was not leaking from the micro-beads and was further kinetically characterized for pyrogallol oxidation. Km for pyrogallol was increased after immobilization from 1.93 mM for soluble HRP to 734 mM for immobilized HRP. The optimum pH was also increased from pH 7.0 to 8.0. Temperature and organic solvent stability improved significantly after immobilization, so that half-life at 70 degrees C increased around four times, while half-life in 80% (v/v) dioxane increased 22 times. After repeated use of 6 times in batch reactor for pyrogallol oxidation immobilized HRP retained 45% of original activity.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Reactive and Functional Polymers",
title = "Tyramine modified alginates via periodate oxidation for peroxidase induced hydrogel formation and immobilization",
volume = "93",
pages = "77-83",
doi = "10.1016/j.reactfunctpolym.2015.06.004"
}
Prodanović, O., Spasojević, D., Prokopijević, M., Radotić, K., Marković, N., Blažić, M.,& Prodanović, R. (2015). Tyramine modified alginates via periodate oxidation for peroxidase induced hydrogel formation and immobilization.
Reactive and Functional Polymers
Elsevier Science Bv, Amsterdam., 93, 77-83.
https://doi.org/10.1016/j.reactfunctpolym.2015.06.004
Prodanović O, Spasojević D, Prokopijević M, Radotić K, Marković N, Blažić M, Prodanović R. Tyramine modified alginates via periodate oxidation for peroxidase induced hydrogel formation and immobilization. Reactive and Functional Polymers. 2015;93:77-83
Prodanović Olivera, Spasojević Dragica, Prokopijević Miloš, Radotić Ksenija, Marković Nevena, Blažić Marija, Prodanović Radivoje, "Tyramine modified alginates via periodate oxidation for peroxidase induced hydrogel formation and immobilization" Reactive and Functional Polymers, 93 (2015):77-83,
https://doi.org/10.1016/j.reactfunctpolym.2015.06.004 .
19
13
18

Supplementary material for the article: Pejin, B.; Nakarada, D.; Novakovic, M.; Tesevic, V.; Savic, A.; Radotic, K.; Mojovic, M. Antioxidant Volatiles of the Freshwater Bryozoan Hyalinella Punctata. Natural Product Research 2014, 28 (18), 1471–1475. https://doi.org/10.1080/14786419.2014.905565

Pejin, Boris; Nakarada, Djura; Novaković, Miroslav M.; Tešević, Vele; Savić, Aleksandar; Radotić, Ksenija; Mojović, Miloš

(Taylor & Francis Ltd, Abingdon, 2014)

TY  - BOOK
AU  - Pejin, Boris
AU  - Nakarada, Djura
AU  - Novaković, Miroslav M.
AU  - Tešević, Vele
AU  - Savić, Aleksandar
AU  - Radotić, Ksenija
AU  - Mojović, Miloš
PY  - 2014
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3672
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Supplementary material for the article: Pejin, B.; Nakarada, D.; Novakovic, M.; Tesevic, V.; Savic, A.; Radotic, K.; Mojovic, M.  Antioxidant Volatiles of the Freshwater Bryozoan Hyalinella Punctata. Natural Product  Research 2014, 28 (18), 1471–1475. https://doi.org/10.1080/14786419.2014.905565
ER  - 
@book{
author = "Pejin, Boris and Nakarada, Djura and Novaković, Miroslav M. and Tešević, Vele and Savić, Aleksandar and Radotić, Ksenija and Mojović, Miloš",
year = "2014",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3672",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Supplementary material for the article: Pejin, B.; Nakarada, D.; Novakovic, M.; Tesevic, V.; Savic, A.; Radotic, K.; Mojovic, M.  Antioxidant Volatiles of the Freshwater Bryozoan Hyalinella Punctata. Natural Product  Research 2014, 28 (18), 1471–1475. https://doi.org/10.1080/14786419.2014.905565"
}
Pejin, B., Nakarada, D., Novaković, M. M., Tešević, V., Savić, A., Radotić, K.,& Mojović, M. (2014). Supplementary material for the article: Pejin, B.; Nakarada, D.; Novakovic, M.; Tesevic, V.; Savic, A.; Radotic, K.; Mojovic, M.  Antioxidant Volatiles of the Freshwater Bryozoan Hyalinella Punctata. Natural Product  Research 2014, 28 (18), 1471–1475. https://doi.org/10.1080/14786419.2014.905565.
Natural Product Research
Taylor & Francis Ltd, Abingdon..
Pejin B, Nakarada D, Novaković MM, Tešević V, Savić A, Radotić K, Mojović M. Supplementary material for the article: Pejin, B.; Nakarada, D.; Novakovic, M.; Tesevic, V.; Savic, A.; Radotic, K.; Mojovic, M.  Antioxidant Volatiles of the Freshwater Bryozoan Hyalinella Punctata. Natural Product  Research 2014, 28 (18), 1471–1475. https://doi.org/10.1080/14786419.2014.905565. Natural Product Research. 2014;
Pejin Boris, Nakarada Djura, Novaković Miroslav M., Tešević Vele, Savić Aleksandar, Radotić Ksenija, Mojović Miloš, "Supplementary material for the article: Pejin, B.; Nakarada, D.; Novakovic, M.; Tesevic, V.; Savic, A.; Radotic, K.; Mojovic, M.  Antioxidant Volatiles of the Freshwater Bryozoan Hyalinella Punctata. Natural Product  Research 2014, 28 (18), 1471–1475. https://doi.org/10.1080/14786419.2014.905565" Natural Product Research (2014)

Soybean hull peroxidase immobilization on macroporous glycidyl methacrylates with different surface characteristics

Prokopijević, Miloš; Prodanović, Olivera; Spasojević, Dragica; Stojanović, Željko; Radotić, Ksenija; Prodanović, Radivoje

(Springer, New York, 2014)

TY  - JOUR
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Spasojević, Dragica
AU  - Stojanović, Željko
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2014
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1768
AB  - Soybean hull peroxidase (SHP, E.C. 1.11.1.7) was immobilized by a glutaraldehyde and periodate method onto series of macroporous copolymers of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA), poly(GMA-co-EGDMA) with various surface characteristics and pore size diameters ranging from 44 to 200 nm. Glutaraldehyde immobilization method and poly(GMA-co-EGDMA) named SGE 20/12 with pore sizes of 120 nm gave immobilized enzyme with highest specific activity of 25 U/g. Deactivation studies showed that immobilization increased stability of SHP and that surface characteristics of the used copolymer had a major influence on a stability of immobilized enzyme at high temperatures and in an organic solvent. The highest thermostability was obtained using the copolymer SGE 20/12 with pore size of 120 nm, while the highest stability in dioxane had SHP immobilized onto copolymer SGE 10/4 with pore size of 44 nm. Immobilized SHP showed a wider pH optimum as compared to the native enzyme especially at alkaline pH values and 3.2 times increased K (m) value for pyrogallol. After 6 cycles of repeated use in batch reactor, immobilized SHP retained 25 % of its original activity. Macroporous copolymers with different surface characteristics can be used for fine tuning of activity and stability of immobilized SHP to obtain a biocatalyst suitable for phenol oxidation or polymer synthesis in organic solvents.
PB  - Springer, New York
T2  - Bioprocess and Biosystems Engineering
T1  - Soybean hull peroxidase immobilization on macroporous glycidyl methacrylates with different surface characteristics
VL  - 37
IS  - 5
SP  - 799
EP  - 804
DO  - 10.1007/s00449-013-1050-z
ER  - 
@article{
author = "Prokopijević, Miloš and Prodanović, Olivera and Spasojević, Dragica and Stojanović, Željko and Radotić, Ksenija and Prodanović, Radivoje",
year = "2014",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1768",
abstract = "Soybean hull peroxidase (SHP, E.C. 1.11.1.7) was immobilized by a glutaraldehyde and periodate method onto series of macroporous copolymers of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA), poly(GMA-co-EGDMA) with various surface characteristics and pore size diameters ranging from 44 to 200 nm. Glutaraldehyde immobilization method and poly(GMA-co-EGDMA) named SGE 20/12 with pore sizes of 120 nm gave immobilized enzyme with highest specific activity of 25 U/g. Deactivation studies showed that immobilization increased stability of SHP and that surface characteristics of the used copolymer had a major influence on a stability of immobilized enzyme at high temperatures and in an organic solvent. The highest thermostability was obtained using the copolymer SGE 20/12 with pore size of 120 nm, while the highest stability in dioxane had SHP immobilized onto copolymer SGE 10/4 with pore size of 44 nm. Immobilized SHP showed a wider pH optimum as compared to the native enzyme especially at alkaline pH values and 3.2 times increased K (m) value for pyrogallol. After 6 cycles of repeated use in batch reactor, immobilized SHP retained 25 % of its original activity. Macroporous copolymers with different surface characteristics can be used for fine tuning of activity and stability of immobilized SHP to obtain a biocatalyst suitable for phenol oxidation or polymer synthesis in organic solvents.",
publisher = "Springer, New York",
journal = "Bioprocess and Biosystems Engineering",
title = "Soybean hull peroxidase immobilization on macroporous glycidyl methacrylates with different surface characteristics",
volume = "37",
number = "5",
pages = "799-804",
doi = "10.1007/s00449-013-1050-z"
}
Prokopijević, M., Prodanović, O., Spasojević, D., Stojanović, Ž., Radotić, K.,& Prodanović, R. (2014). Soybean hull peroxidase immobilization on macroporous glycidyl methacrylates with different surface characteristics.
Bioprocess and Biosystems Engineering
Springer, New York., 37(5), 799-804.
https://doi.org/10.1007/s00449-013-1050-z
Prokopijević M, Prodanović O, Spasojević D, Stojanović Ž, Radotić K, Prodanović R. Soybean hull peroxidase immobilization on macroporous glycidyl methacrylates with different surface characteristics. Bioprocess and Biosystems Engineering. 2014;37(5):799-804
Prokopijević Miloš, Prodanović Olivera, Spasojević Dragica, Stojanović Željko, Radotić Ksenija, Prodanović Radivoje, "Soybean hull peroxidase immobilization on macroporous glycidyl methacrylates with different surface characteristics" Bioprocess and Biosystems Engineering, 37, no. 5 (2014):799-804,
https://doi.org/10.1007/s00449-013-1050-z .
14
11
13

Immobilization of chemically modified horseradish peroxidase within activated alginate beads

Spasojević, Dragica; Prokopijević, Miloš; Prodanović, Olivera; Pirtea, Marilen Gabriel; Radotić, Ksenija; Prodanović, Radivoje

(Assoc Chemical Engineers Serbia, Belgrade, 2014)

TY  - JOUR
AU  - Spasojević, Dragica
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Pirtea, Marilen Gabriel
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2014
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1770
AB  - Immobilization of horseradish peroxidase (HRP) within alginate beads was enabled by chemical modification of the enzyme and polysaccharide chains. HRP and alginate were oxidized by periodate and subsequently modified with ethylenediamine. Highest specific activity of 0.43 U/ml of gel and 81% of bound enzyme activity was obtained using aminated HRP and alginate oxidized by periodate. Immobilized enzyme retained 75% of its original activity after 2 days of incubation in 80% (v/v) dioxane and had increased activity in basic solutions compared to native enzyme. During repeated use in batch reactor for pyrogallol oxidation immobilized peroxidase retained 75% of its original activity.
AB  - Imobilizacija peroksidaze iz rena unutar alginatnih kuglica je poboljšana hemijskom modifikacijom enzima i polisaharidnih lanaca. Peroksidaza i alginat su oksidovani perjodatom i naknadno modifikovani etilendiaminom. Najveća specifična aktivnost od 0,43 U/ml gela i 81% vezane aktivnosti je dobijeno korišćenjem aminovane peroksidaze i alginata oksidovanog perjodatom. Imobilizovani enzim je zadržao 75% originalne aktivnosti nakon 2 dana inkubacije u 80% (v/v) dioksanu i imao je povećanu aktivnost pri baznim pH vrednostima u poređenju sa nativnim enzimom. Tokom višestruke upotrebe u šaržnom reaktoru za oksidaciju pirogalola imobilizovana peroksidaza je zadržala 75% početne aktivnosti.
PB  - Assoc Chemical Engineers Serbia, Belgrade
T2  - Hemijska industrija
T1  - Immobilization of chemically modified horseradish peroxidase within activated alginate beads
T1  - Imobilizacija hemijski modifikovane peroksidaze iz rena unutar aktiviranih alginatnih kuglica
VL  - 68
IS  - 1
SP  - 117
EP  - 122
DO  - 10.2298/HEMIND121122036S
ER  - 
@article{
author = "Spasojević, Dragica and Prokopijević, Miloš and Prodanović, Olivera and Pirtea, Marilen Gabriel and Radotić, Ksenija and Prodanović, Radivoje",
year = "2014",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1770",
abstract = "Immobilization of horseradish peroxidase (HRP) within alginate beads was enabled by chemical modification of the enzyme and polysaccharide chains. HRP and alginate were oxidized by periodate and subsequently modified with ethylenediamine. Highest specific activity of 0.43 U/ml of gel and 81% of bound enzyme activity was obtained using aminated HRP and alginate oxidized by periodate. Immobilized enzyme retained 75% of its original activity after 2 days of incubation in 80% (v/v) dioxane and had increased activity in basic solutions compared to native enzyme. During repeated use in batch reactor for pyrogallol oxidation immobilized peroxidase retained 75% of its original activity., Imobilizacija peroksidaze iz rena unutar alginatnih kuglica je poboljšana hemijskom modifikacijom enzima i polisaharidnih lanaca. Peroksidaza i alginat su oksidovani perjodatom i naknadno modifikovani etilendiaminom. Najveća specifična aktivnost od 0,43 U/ml gela i 81% vezane aktivnosti je dobijeno korišćenjem aminovane peroksidaze i alginata oksidovanog perjodatom. Imobilizovani enzim je zadržao 75% originalne aktivnosti nakon 2 dana inkubacije u 80% (v/v) dioksanu i imao je povećanu aktivnost pri baznim pH vrednostima u poređenju sa nativnim enzimom. Tokom višestruke upotrebe u šaržnom reaktoru za oksidaciju pirogalola imobilizovana peroksidaza je zadržala 75% početne aktivnosti.",
publisher = "Assoc Chemical Engineers Serbia, Belgrade",
journal = "Hemijska industrija",
title = "Immobilization of chemically modified horseradish peroxidase within activated alginate beads, Imobilizacija hemijski modifikovane peroksidaze iz rena unutar aktiviranih alginatnih kuglica",
volume = "68",
number = "1",
pages = "117-122",
doi = "10.2298/HEMIND121122036S"
}
Spasojević, D., Prokopijević, M., Prodanović, O., Pirtea, M. G., Radotić, K.,& Prodanović, R. (2014). Imobilizacija hemijski modifikovane peroksidaze iz rena unutar aktiviranih alginatnih kuglica.
Hemijska industrija
Assoc Chemical Engineers Serbia, Belgrade., 68(1), 117-122.
https://doi.org/10.2298/HEMIND121122036S
Spasojević D, Prokopijević M, Prodanović O, Pirtea MG, Radotić K, Prodanović R. Imobilizacija hemijski modifikovane peroksidaze iz rena unutar aktiviranih alginatnih kuglica. Hemijska industrija. 2014;68(1):117-122
Spasojević Dragica, Prokopijević Miloš, Prodanović Olivera, Pirtea Marilen Gabriel, Radotić Ksenija, Prodanović Radivoje, "Imobilizacija hemijski modifikovane peroksidaze iz rena unutar aktiviranih alginatnih kuglica" Hemijska industrija, 68, no. 1 (2014):117-122,
https://doi.org/10.2298/HEMIND121122036S .
6
6
7

Antioxidant volatiles of the freshwater bryozoan Hyalinella punctata

Pejin, Boris; Nakarada, Djura; Novaković, Miroslav M.; Tešević, Vele; Savić, Aleksandar; Radotić, Ksenija; Mojović, Miloš

(Taylor & Francis Ltd, Abingdon, 2014)

TY  - JOUR
AU  - Pejin, Boris
AU  - Nakarada, Djura
AU  - Novaković, Miroslav M.
AU  - Tešević, Vele
AU  - Savić, Aleksandar
AU  - Radotić, Ksenija
AU  - Mojović, Miloš
PY  - 2014
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1828
AB  - Two volatile samples of the bryozoan Hyalinella punctata (seasonally collected from the same locality) were isolated (hydrodistillation, Clevenger apparatus), identified (GC and GC-MS) and screened in vitro (EPR) for anti-hydroxyl radical activity. The main components of sample 1 (2-ethyl-1-hexanol 37.00%, dodecanol 21.40% and hexanal 8.40%) and sample 2 (2-ethyl-1-hexanol 30.50%, 7-tridecanol 24.60% and 1-hexadecanol 11.80%) were relatively similar. However, more components were present in the sample 2 (17 and 25, respectively). EPR measurements indicated significant anti-hydroxyl radical activity of the both samples (75.00 +/- 6.00% and 87.00 +/- 8.00%, respectively) whereas the generation of other types of free radicals in reaction with hydroxyl radicals was not observed. According to the best of our knowledge, this is the first report of 31 organic compounds from the phylum Bryozoa. Alcohols, aldehydes, ketones, esters and ethers of lower molecular mass appear to be characteristic for the volatiles of these organisms commonly known as moss animals.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Antioxidant volatiles of the freshwater bryozoan Hyalinella punctata
VL  - 28
IS  - 18
SP  - 1471
EP  - 1475
DO  - 10.1080/14786419.2014.905565
ER  - 
@article{
author = "Pejin, Boris and Nakarada, Djura and Novaković, Miroslav M. and Tešević, Vele and Savić, Aleksandar and Radotić, Ksenija and Mojović, Miloš",
year = "2014",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1828",
abstract = "Two volatile samples of the bryozoan Hyalinella punctata (seasonally collected from the same locality) were isolated (hydrodistillation, Clevenger apparatus), identified (GC and GC-MS) and screened in vitro (EPR) for anti-hydroxyl radical activity. The main components of sample 1 (2-ethyl-1-hexanol 37.00%, dodecanol 21.40% and hexanal 8.40%) and sample 2 (2-ethyl-1-hexanol 30.50%, 7-tridecanol 24.60% and 1-hexadecanol 11.80%) were relatively similar. However, more components were present in the sample 2 (17 and 25, respectively). EPR measurements indicated significant anti-hydroxyl radical activity of the both samples (75.00 +/- 6.00% and 87.00 +/- 8.00%, respectively) whereas the generation of other types of free radicals in reaction with hydroxyl radicals was not observed. According to the best of our knowledge, this is the first report of 31 organic compounds from the phylum Bryozoa. Alcohols, aldehydes, ketones, esters and ethers of lower molecular mass appear to be characteristic for the volatiles of these organisms commonly known as moss animals.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Antioxidant volatiles of the freshwater bryozoan Hyalinella punctata",
volume = "28",
number = "18",
pages = "1471-1475",
doi = "10.1080/14786419.2014.905565"
}
Pejin, B., Nakarada, D., Novaković, M. M., Tešević, V., Savić, A., Radotić, K.,& Mojović, M. (2014). Antioxidant volatiles of the freshwater bryozoan Hyalinella punctata.
Natural Product Research
Taylor & Francis Ltd, Abingdon., 28(18), 1471-1475.
https://doi.org/10.1080/14786419.2014.905565
Pejin B, Nakarada D, Novaković MM, Tešević V, Savić A, Radotić K, Mojović M. Antioxidant volatiles of the freshwater bryozoan Hyalinella punctata. Natural Product Research. 2014;28(18):1471-1475
Pejin Boris, Nakarada Djura, Novaković Miroslav M., Tešević Vele, Savić Aleksandar, Radotić Ksenija, Mojović Miloš, "Antioxidant volatiles of the freshwater bryozoan Hyalinella punctata" Natural Product Research, 28, no. 18 (2014):1471-1475,
https://doi.org/10.1080/14786419.2014.905565 .
3
3
3

Differentiation of wine commercial samples by using fluorescence spectroscopy and multivariate analysis

Mutavdžić, Mira; Mutavdžić, Dragosav; Radotić, Ksenija; Milojković-Opsenica, Dušanka

(2013)

TY  - JOUR
AU  - Mutavdžić, Mira
AU  - Mutavdžić, Dragosav
AU  - Radotić, Ksenija
AU  - Milojković-Opsenica, Dušanka
PY  - 2013
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/88
AB  - Steady state fluorescence spectroscopy in combination with Multivariate Curve Resolution - Alternating Least Squares (MCR-ALS) for spectral analysis has been applied to differentiate commercial samples of wines available on the market. We have chosen the wine trade marks from the two Serbian producers that contain only one wine type, since such approach is a starting step for further analyses of the more complex samples containing different wine types. We also studied changes of the emission spectra of these samples during seven days after opening of the wine bottle. The emission spectra were recorded in the wavelength range 275 - 500 nm, after excitation in the range 255 - 300 nm. The spectra of the wine samples obtained from the same producer are very similar, i.e. contain the same components in the similar ratios. The changes of spectral components at 315 nm and 430 nm were the basis for differentiation of the wine samples from the two producers, as well as for estimation of the wine stability in time after the bottle opening. The results indicate that this may be a useful approach in fingerprinting wines from various producers, as well as in screening the stability of wine.
AB  - Fluorescentna spektroskopija u kombinaciji sa metodom Multivarijaciona Rezolucija Krivih - Naizmenični Najmanji Kvadrati (Multivariate Curve Resolution - Alternating Least Squares, MCR-ALS), je primenjena za razlikovanje komercijalnih uzoraka vina dostupnih na tržištu. Odabrali smo trgovačke marke vina od dva srpska proizvođača koje sadrže samo jednu vrstu vina, jer je ovakav pristup početni korak za kasnije analize složenijih uzoraka koji sadrže različite vrste vina. Takođe smo proučavali promene emisionih spektara ovih uzoraka u toku sedam dana posle otvaranja boce. Emisioni spektri su mereni u opsegu talasnih dužina 275 - 500 nm, posle pobuđivanja u opsegu 255 - 300 nm. Spektri uzoraka vina od istog proizvođača su veoma slični, to jest sadrže iste komponente u sličnom međusobnom odnosu. Promene komponenata spektara na 315 nm i 430 nm bile su osnova za razlikovanje uzoraka vina od dva proizvođača, kao i za procenu stabilnosti vina u vremenu posle otvaranja boce. Rezultati ukazuju da ovo može biti koristan pristup u razlikovanju vina od različitih proizvođača, kao i u praćenju stabilnosti sastava vina.
T2  - Acta agriculturae Serbica
T1  - Differentiation of wine commercial samples by using fluorescence spectroscopy and multivariate analysis
T1  - Razlikovanje komercijalnih uzoraka vina korišćenjem fluorescentne spektroskopije i multivarijacione analize
VL  - 18
IS  - 36
SP  - 169
EP  - 177
ER  - 
@article{
author = "Mutavdžić, Mira and Mutavdžić, Dragosav and Radotić, Ksenija and Milojković-Opsenica, Dušanka",
year = "2013",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/88",
abstract = "Steady state fluorescence spectroscopy in combination with Multivariate Curve Resolution - Alternating Least Squares (MCR-ALS) for spectral analysis has been applied to differentiate commercial samples of wines available on the market. We have chosen the wine trade marks from the two Serbian producers that contain only one wine type, since such approach is a starting step for further analyses of the more complex samples containing different wine types. We also studied changes of the emission spectra of these samples during seven days after opening of the wine bottle. The emission spectra were recorded in the wavelength range 275 - 500 nm, after excitation in the range 255 - 300 nm. The spectra of the wine samples obtained from the same producer are very similar, i.e. contain the same components in the similar ratios. The changes of spectral components at 315 nm and 430 nm were the basis for differentiation of the wine samples from the two producers, as well as for estimation of the wine stability in time after the bottle opening. The results indicate that this may be a useful approach in fingerprinting wines from various producers, as well as in screening the stability of wine., Fluorescentna spektroskopija u kombinaciji sa metodom Multivarijaciona Rezolucija Krivih - Naizmenični Najmanji Kvadrati (Multivariate Curve Resolution - Alternating Least Squares, MCR-ALS), je primenjena za razlikovanje komercijalnih uzoraka vina dostupnih na tržištu. Odabrali smo trgovačke marke vina od dva srpska proizvođača koje sadrže samo jednu vrstu vina, jer je ovakav pristup početni korak za kasnije analize složenijih uzoraka koji sadrže različite vrste vina. Takođe smo proučavali promene emisionih spektara ovih uzoraka u toku sedam dana posle otvaranja boce. Emisioni spektri su mereni u opsegu talasnih dužina 275 - 500 nm, posle pobuđivanja u opsegu 255 - 300 nm. Spektri uzoraka vina od istog proizvođača su veoma slični, to jest sadrže iste komponente u sličnom međusobnom odnosu. Promene komponenata spektara na 315 nm i 430 nm bile su osnova za razlikovanje uzoraka vina od dva proizvođača, kao i za procenu stabilnosti vina u vremenu posle otvaranja boce. Rezultati ukazuju da ovo može biti koristan pristup u razlikovanju vina od različitih proizvođača, kao i u praćenju stabilnosti sastava vina.",
journal = "Acta agriculturae Serbica",
title = "Differentiation of wine commercial samples by using fluorescence spectroscopy and multivariate analysis, Razlikovanje komercijalnih uzoraka vina korišćenjem fluorescentne spektroskopije i multivarijacione analize",
volume = "18",
number = "36",
pages = "169-177"
}
Mutavdžić, M., Mutavdžić, D., Radotić, K.,& Milojković-Opsenica, D. (2013). Razlikovanje komercijalnih uzoraka vina korišćenjem fluorescentne spektroskopije i multivarijacione analize.
Acta agriculturae Serbica, 18(36), 169-177.
Mutavdžić M, Mutavdžić D, Radotić K, Milojković-Opsenica D. Razlikovanje komercijalnih uzoraka vina korišćenjem fluorescentne spektroskopije i multivarijacione analize. Acta agriculturae Serbica. 2013;18(36):169-177
Mutavdžić Mira, Mutavdžić Dragosav, Radotić Ksenija, Milojković-Opsenica Dušanka, "Razlikovanje komercijalnih uzoraka vina korišćenjem fluorescentne spektroskopije i multivarijacione analize" Acta agriculturae Serbica, 18, no. 36 (2013):169-177

Antimicrobial Activity of the Freshwater Bryozoan Hyalinella Punctata (Hancock, 1850)

Pejin, Boris; Glamočlija, Jasmina; Ćirić, Ana D.; Radotić, Ksenija; Vajs, Vlatka; Tešević, Vele; Hegedis, Aleksandar; Karaman, Ivo; Horvatovic, Mladen; Soković, Marina

(Inst Materials Physics, Bucharest, 2012)

TY  - JOUR
AU  - Pejin, Boris
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana D.
AU  - Radotić, Ksenija
AU  - Vajs, Vlatka
AU  - Tešević, Vele
AU  - Hegedis, Aleksandar
AU  - Karaman, Ivo
AU  - Horvatovic, Mladen
AU  - Soković, Marina
PY  - 2012
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1565
AB  - The antimicrobial activity of the freshwater bryozoan Hyalinella punctata (Hancock, 1850) was tested by microdilution method against eight bacteria and eight fungi for the first time. All five crude extracts (hexane, acetone, dimethyl sulfoxide, methanol and water) showed good antibacterial and antifungal potential in vitro wherein the acetone extract was the most active (MICs 0.50-7.00 mu g/ml and MBCs 2.50-10.00 mu g/ml).
PB  - Inst Materials Physics, Bucharest
T2  - Digest Journal of Nanomaterials and Biostructures
T1  - Antimicrobial Activity of the Freshwater Bryozoan Hyalinella Punctata (Hancock, 1850)
VL  - 7
IS  - 3
SP  - 1021
EP  - 1026
ER  - 
@article{
author = "Pejin, Boris and Glamočlija, Jasmina and Ćirić, Ana D. and Radotić, Ksenija and Vajs, Vlatka and Tešević, Vele and Hegedis, Aleksandar and Karaman, Ivo and Horvatovic, Mladen and Soković, Marina",
year = "2012",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1565",
abstract = "The antimicrobial activity of the freshwater bryozoan Hyalinella punctata (Hancock, 1850) was tested by microdilution method against eight bacteria and eight fungi for the first time. All five crude extracts (hexane, acetone, dimethyl sulfoxide, methanol and water) showed good antibacterial and antifungal potential in vitro wherein the acetone extract was the most active (MICs 0.50-7.00 mu g/ml and MBCs 2.50-10.00 mu g/ml).",
publisher = "Inst Materials Physics, Bucharest",
journal = "Digest Journal of Nanomaterials and Biostructures",
title = "Antimicrobial Activity of the Freshwater Bryozoan Hyalinella Punctata (Hancock, 1850)",
volume = "7",
number = "3",
pages = "1021-1026"
}
Pejin, B., Glamočlija, J., Ćirić, A. D., Radotić, K., Vajs, V., Tešević, V., Hegedis, A., Karaman, I., Horvatovic, M.,& Soković, M. (2012). Antimicrobial Activity of the Freshwater Bryozoan Hyalinella Punctata (Hancock, 1850).
Digest Journal of Nanomaterials and Biostructures
Inst Materials Physics, Bucharest., 7(3), 1021-1026.
Pejin B, Glamočlija J, Ćirić AD, Radotić K, Vajs V, Tešević V, Hegedis A, Karaman I, Horvatovic M, Soković M. Antimicrobial Activity of the Freshwater Bryozoan Hyalinella Punctata (Hancock, 1850). Digest Journal of Nanomaterials and Biostructures. 2012;7(3):1021-1026
Pejin Boris, Glamočlija Jasmina, Ćirić Ana D., Radotić Ksenija, Vajs Vlatka, Tešević Vele, Hegedis Aleksandar, Karaman Ivo, Horvatovic Mladen, Soković Marina, "Antimicrobial Activity of the Freshwater Bryozoan Hyalinella Punctata (Hancock, 1850)" Digest Journal of Nanomaterials and Biostructures, 7, no. 3 (2012):1021-1026
7
7

Improved Covalent Immobilization of Horseradish Peroxidase on Macroporous Glycidyl Methacrylate-Based Copolymers

Prodanović, Olivera; Prokopijević, Miloš; Spasojević, Dragica; Stojanović, Željko; Radotić, Ksenija; Knezevic-Jugovic, Zorica D.; Prodanović, Radivoje

(Humana Press Inc, Totowa, 2012)

TY  - JOUR
AU  - Prodanović, Olivera
AU  - Prokopijević, Miloš
AU  - Spasojević, Dragica
AU  - Stojanović, Željko
AU  - Radotić, Ksenija
AU  - Knezevic-Jugovic, Zorica D.
AU  - Prodanović, Radivoje
PY  - 2012
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/1552
AB  - A macroporous copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate, poly(GMA-co-EGDMA), with various surface characteristics and mean pore size diameters ranging from 44 to 200 nm was synthesized, modified with 1,2-diaminoethane, and tested as a carrier for immobilization of horseradish peroxidase (HRP) by two covalent methods, glutaraldehyde and periodate. The highest specific activity of around 35 U g(-1) dry weight of carrier was achieved on poly(GMA-co-EGDMA) copolymers with mean pore diameters of 200 and 120 nm by the periodate method. A study of deactivation kinetics at 65 A degrees C and in 80 % dioxane revealed that periodate immobilization also produced an appreciable stabilization of the biocatalyst, while stabilization factor depended strongly on the surface characteristics of the copolymers. HRP immobilized on copolymer with a mean pore diameter of 120 nm by periodate method showing not only the highest specific activity but also good stability was further characterized. It appeared that the immobilization resulted in the stabilization of enzyme over a broader pH range while the Michaelis constant value (K (m)) of the immobilized HRP was 10.8 mM, approximately 5.6 times higher than that of the free enzyme. After 6 cycles of repeated use in a batch reactor for pyrogallol oxidation, the immobilized HRP retained 45 % of its original activity.
PB  - Humana Press Inc, Totowa
T2  - Applied Biochemistry and Biotechnology
T1  - Improved Covalent Immobilization of Horseradish Peroxidase on Macroporous Glycidyl Methacrylate-Based Copolymers
VL  - 168
IS  - 5
SP  - 1288
EP  - 1301
DO  - 10.1007/s12010-012-9857-7
ER  - 
@article{
author = "Prodanović, Olivera and Prokopijević, Miloš and Spasojević, Dragica and Stojanović, Željko and Radotić, Ksenija and Knezevic-Jugovic, Zorica D. and Prodanović, Radivoje",
year = "2012",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/1552",
abstract = "A macroporous copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate, poly(GMA-co-EGDMA), with various surface characteristics and mean pore size diameters ranging from 44 to 200 nm was synthesized, modified with 1,2-diaminoethane, and tested as a carrier for immobilization of horseradish peroxidase (HRP) by two covalent methods, glutaraldehyde and periodate. The highest specific activity of around 35 U g(-1) dry weight of carrier was achieved on poly(GMA-co-EGDMA) copolymers with mean pore diameters of 200 and 120 nm by the periodate method. A study of deactivation kinetics at 65 A degrees C and in 80 % dioxane revealed that periodate immobilization also produced an appreciable stabilization of the biocatalyst, while stabilization factor depended strongly on the surface characteristics of the copolymers. HRP immobilized on copolymer with a mean pore diameter of 120 nm by periodate method showing not only the highest specific activity but also good stability was further characterized. It appeared that the immobilization resulted in the stabilization of enzyme over a broader pH range while the Michaelis constant value (K (m)) of the immobilized HRP was 10.8 mM, approximately 5.6 times higher than that of the free enzyme. After 6 cycles of repeated use in a batch reactor for pyrogallol oxidation, the immobilized HRP retained 45 % of its original activity.",
publisher = "Humana Press Inc, Totowa",
journal = "Applied Biochemistry and Biotechnology",
title = "Improved Covalent Immobilization of Horseradish Peroxidase on Macroporous Glycidyl Methacrylate-Based Copolymers",
volume = "168",
number = "5",
pages = "1288-1301",
doi = "10.1007/s12010-012-9857-7"
}
Prodanović, O., Prokopijević, M., Spasojević, D., Stojanović, Ž., Radotić, K., Knezevic-Jugovic, Z. D.,& Prodanović, R. (2012). Improved Covalent Immobilization of Horseradish Peroxidase on Macroporous Glycidyl Methacrylate-Based Copolymers.
Applied Biochemistry and Biotechnology
Humana Press Inc, Totowa., 168(5), 1288-1301.
https://doi.org/10.1007/s12010-012-9857-7
Prodanović O, Prokopijević M, Spasojević D, Stojanović Ž, Radotić K, Knezevic-Jugovic ZD, Prodanović R. Improved Covalent Immobilization of Horseradish Peroxidase on Macroporous Glycidyl Methacrylate-Based Copolymers. Applied Biochemistry and Biotechnology. 2012;168(5):1288-1301
Prodanović Olivera, Prokopijević Miloš, Spasojević Dragica, Stojanović Željko, Radotić Ksenija, Knezevic-Jugovic Zorica D., Prodanović Radivoje, "Improved Covalent Immobilization of Horseradish Peroxidase on Macroporous Glycidyl Methacrylate-Based Copolymers" Applied Biochemistry and Biotechnology, 168, no. 5 (2012):1288-1301,
https://doi.org/10.1007/s12010-012-9857-7 .
16
15
19