TY  - CONF
AU  - Waden, Konrad
AU  - Polović, Natalija
AU  - Binnmyr, J.
AU  - Hamsten, Carl
AU  - Gronneberg, R.
AU  - Palmberg, C.
AU  - Milčić-Matić, Natalija
AU  - Bergman, T.
AU  - Gronlund, Hans
AU  - van Hage, Marianne
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1409
AB  - Background Allergy to dog (Canis familiaris) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of IgE-mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. Methods IgE-binding proteins in dog saliva and dander extract were analysed by immunoblot and mass spectrometry (LC-MS/MS) using pooled or individual sera from dog-allergic patients (n = 13). Sera from 59 patients IgE positive to dander and 55 patients IgE negative to dander but with symptoms to dog were analysed for IgE against saliva and dander by ELISA. Basophil stimulation with dog saliva and dander extract was measured by flow cytometry among three dog-allergic patients. Additionally, IgE-binding protein profiles of saliva from different breeds were investigated by immunoblot. Results Greater number and diversity of IgE-binding proteins was found in saliva compared to dander extract and varied among dog breeds. In saliva, Can f 1, 2, 3 and 6 were identified but also four new saliva allergen candidates. The majority of the 59 dog dander-positive sera (n = 44) were IgE positive to dog saliva. Among patients IgE negative to dander, but with symptoms to dog, 20% were IgE positive to saliva. The biological activity of saliva was confirmed by basophil degranulation. Conclusions Dog saliva is an allergen source for improved diagnostics of dog allergy. The IgE-binding protein profile of saliva from different dogs varies. © 2013 John Wiley & Sons A/S. Published by Blackwell Publishing Ltd.
PB  - Wiley-Blackwell, Hoboken
C3  - Allergy
T1  - Dog saliva - an important source of dog allergens
VL  - 68
SP  - 35
EP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_cherry_1409
ER  - 

@conference{
author = "Waden, Konrad and Polović, Natalija and Binnmyr, J. and Hamsten, Carl and Gronneberg, R. and Palmberg, C. and Milčić-Matić, Natalija and Bergman, T. and Gronlund, Hans and van Hage, Marianne",
year = "2013",
abstract = "Background Allergy to dog (Canis familiaris) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of IgE-mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. Methods IgE-binding proteins in dog saliva and dander extract were analysed by immunoblot and mass spectrometry (LC-MS/MS) using pooled or individual sera from dog-allergic patients (n = 13). Sera from 59 patients IgE positive to dander and 55 patients IgE negative to dander but with symptoms to dog were analysed for IgE against saliva and dander by ELISA. Basophil stimulation with dog saliva and dander extract was measured by flow cytometry among three dog-allergic patients. Additionally, IgE-binding protein profiles of saliva from different breeds were investigated by immunoblot. Results Greater number and diversity of IgE-binding proteins was found in saliva compared to dander extract and varied among dog breeds. In saliva, Can f 1, 2, 3 and 6 were identified but also four new saliva allergen candidates. The majority of the 59 dog dander-positive sera (n = 44) were IgE positive to dog saliva. Among patients IgE negative to dander, but with symptoms to dog, 20% were IgE positive to saliva. The biological activity of saliva was confirmed by basophil degranulation. Conclusions Dog saliva is an allergen source for improved diagnostics of dog allergy. The IgE-binding protein profile of saliva from different dogs varies. © 2013 John Wiley & Sons A/S. Published by Blackwell Publishing Ltd.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "Dog saliva - an important source of dog allergens",
volume = "68",
pages = "35-35",
url = "https://hdl.handle.net/21.15107/rcub_cherry_1409"
}

Waden, K., Polović, N., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, N., Bergman, T., Gronlund, H.,& van Hage, M.. (2013). Dog saliva - an important source of dog allergens. in Allergy
Wiley-Blackwell, Hoboken., 68, 35-35.
https://hdl.handle.net/21.15107/rcub_cherry_1409

Waden K, Polović N, Binnmyr J, Hamsten C, Gronneberg R, Palmberg C, Milčić-Matić N, Bergman T, Gronlund H, van Hage M. Dog saliva - an important source of dog allergens. in Allergy. 2013;68:35-35.
https://hdl.handle.net/21.15107/rcub_cherry_1409 .

Waden, Konrad, Polović, Natalija, Binnmyr, J., Hamsten, Carl, Gronneberg, R., Palmberg, C., Milčić-Matić, Natalija, Bergman, T., Gronlund, Hans, van Hage, Marianne, "Dog saliva - an important source of dog allergens" in Allergy, 68 (2013):35-35,
https://hdl.handle.net/21.15107/rcub_cherry_1409 .

TY  - JOUR
AU  - van Hage, Marianne
AU  - Polović, Natalija
AU  - Waden, Konrad
AU  - Binnmyr, J.
AU  - Hamsten, Carl
AU  - Gronneberg, R.
AU  - Palmberg, C.
AU  - Milčić-Matić, Natalija
AU  - Bergman, T.
AU  - Gronlund, Hans
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1488
PB  - Wiley-Blackwell, Hoboken
T2  - Allergy
T1  - Diversity of allergens contained in dog saliva REPLY
VL  - 68
IS  - 11
SP  - 1485
EP  - 1486
DO  - 10.1111/all.12264
ER  - 

@article{
author = "van Hage, Marianne and Polović, Natalija and Waden, Konrad and Binnmyr, J. and Hamsten, Carl and Gronneberg, R. and Palmberg, C. and Milčić-Matić, Natalija and Bergman, T. and Gronlund, Hans",
year = "2013",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "Diversity of allergens contained in dog saliva REPLY",
volume = "68",
number = "11",
pages = "1485-1486",
doi = "10.1111/all.12264"
}

van Hage, M., Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, N., Bergman, T.,& Gronlund, H.. (2013). Diversity of allergens contained in dog saliva REPLY. in Allergy
Wiley-Blackwell, Hoboken., 68(11), 1485-1486.
https://doi.org/10.1111/all.12264

van Hage M, Polović N, Waden K, Binnmyr J, Hamsten C, Gronneberg R, Palmberg C, Milčić-Matić N, Bergman T, Gronlund H. Diversity of allergens contained in dog saliva REPLY. in Allergy. 2013;68(11):1485-1486.
doi:10.1111/all.12264 .

van Hage, Marianne, Polović, Natalija, Waden, Konrad, Binnmyr, J., Hamsten, Carl, Gronneberg, R., Palmberg, C., Milčić-Matić, Natalija, Bergman, T., Gronlund, Hans, "Diversity of allergens contained in dog saliva REPLY" in Allergy, 68, no. 11 (2013):1485-1486,
https://doi.org/10.1111/all.12264 . .

TY  - JOUR
AU  - Polović, Natalija
AU  - Waden, Konrad
AU  - Binnmyr, J.
AU  - Hamsten, Carl
AU  - Gronneberg, R.
AU  - Palmberg, C.
AU  - Milčić-Matić, Natalija
AU  - Bergman, T.
AU  - Gronlund, Hans
AU  - van Hage, Marianne
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1634
AB  - Background Allergy to dog (Canis familiaris) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of IgE-mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. Methods IgE-binding proteins in dog saliva and dander extract were analysed by immunoblot and mass spectrometry (LC-MS/MS) using pooled or individual sera from dog-allergic patients (n=13). Sera from 59 patients IgE positive to dander and 55 patients IgE negative to dander but with symptoms to dog were analysed for IgE against saliva and dander by ELISA. Basophil stimulation with dog saliva and dander extract was measured by flow cytometry among three dog-allergic patients. Additionally, IgE-binding protein profiles of saliva from different breeds were investigated by immunoblot. Results Greater number and diversity of IgE-binding proteins was found in saliva compared to dander extract and varied among dog breeds. In saliva, Can f 1, 2, 3 and 6 were identified but also four new saliva allergen candidates. The majority of the 59 dog danderpositive sera (n=44) were IgE positive to dog saliva. Among patients IgE negative to dander, but with symptoms to dog, 20% were IgE positive to saliva. The biological activity of saliva was confirmed by basophil degranulation. Conclusions Dog saliva is an allergen source for improved diagnostics of dog allergy. The IgE-binding protein profile of saliva from different dogs varies.
PB  - Wiley-Blackwell, Hoboken
T2  - Allergy
T1  - Dog saliva - an important source of dog allergens
VL  - 68
IS  - 5
SP  - 585
EP  - 592
DO  - 10.1111/all.12130
ER  - 

@article{
author = "Polović, Natalija and Waden, Konrad and Binnmyr, J. and Hamsten, Carl and Gronneberg, R. and Palmberg, C. and Milčić-Matić, Natalija and Bergman, T. and Gronlund, Hans and van Hage, Marianne",
year = "2013",
abstract = "Background Allergy to dog (Canis familiaris) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of IgE-mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. Methods IgE-binding proteins in dog saliva and dander extract were analysed by immunoblot and mass spectrometry (LC-MS/MS) using pooled or individual sera from dog-allergic patients (n=13). Sera from 59 patients IgE positive to dander and 55 patients IgE negative to dander but with symptoms to dog were analysed for IgE against saliva and dander by ELISA. Basophil stimulation with dog saliva and dander extract was measured by flow cytometry among three dog-allergic patients. Additionally, IgE-binding protein profiles of saliva from different breeds were investigated by immunoblot. Results Greater number and diversity of IgE-binding proteins was found in saliva compared to dander extract and varied among dog breeds. In saliva, Can f 1, 2, 3 and 6 were identified but also four new saliva allergen candidates. The majority of the 59 dog danderpositive sera (n=44) were IgE positive to dog saliva. Among patients IgE negative to dander, but with symptoms to dog, 20% were IgE positive to saliva. The biological activity of saliva was confirmed by basophil degranulation. Conclusions Dog saliva is an allergen source for improved diagnostics of dog allergy. The IgE-binding protein profile of saliva from different dogs varies.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "Dog saliva - an important source of dog allergens",
volume = "68",
number = "5",
pages = "585-592",
doi = "10.1111/all.12130"
}

Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, N., Bergman, T., Gronlund, H.,& van Hage, M.. (2013). Dog saliva - an important source of dog allergens. in Allergy
Wiley-Blackwell, Hoboken., 68(5), 585-592.
https://doi.org/10.1111/all.12130

Polović N, Waden K, Binnmyr J, Hamsten C, Gronneberg R, Palmberg C, Milčić-Matić N, Bergman T, Gronlund H, van Hage M. Dog saliva - an important source of dog allergens. in Allergy. 2013;68(5):585-592.
doi:10.1111/all.12130 .

Polović, Natalija, Waden, Konrad, Binnmyr, J., Hamsten, Carl, Gronneberg, R., Palmberg, C., Milčić-Matić, Natalija, Bergman, T., Gronlund, Hans, van Hage, Marianne, "Dog saliva - an important source of dog allergens" in Allergy, 68, no. 5 (2013):585-592,
https://doi.org/10.1111/all.12130 . .

TY  - DATA
AU  - Polović, Natalija
AU  - Waden, Konrad
AU  - Binnmyr, J.
AU  - Hamsten, Carl
AU  - Gronneberg, R.
AU  - Palmberg, C.
AU  - Milčić-Matić, Natalija
AU  - Bergman, T.
AU  - Gronlund, Hans
AU  - van Hage, Marianne
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3488
PB  - Wiley-Blackwell, Hoboken
T2  - Allergy
T1  - Supplementary data for article: Polović, N.; Waden, K.; Binnmyr, J.; Hamsten, C.; Gronneberg, R.; Palmberg, C.; Milčić-Matić, N.; Bergman, T.; Gronlund, H.; van Hage, M. Dog Saliva - an Important Source of Dog Allergens. Allergy 2013, 68 (5), 585–592. https://doi.org/10.1111/all.12130
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3488
ER  - 

@misc{
author = "Polović, Natalija and Waden, Konrad and Binnmyr, J. and Hamsten, Carl and Gronneberg, R. and Palmberg, C. and Milčić-Matić, Natalija and Bergman, T. and Gronlund, Hans and van Hage, Marianne",
year = "2013",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "Supplementary data for article: Polović, N.; Waden, K.; Binnmyr, J.; Hamsten, C.; Gronneberg, R.; Palmberg, C.; Milčić-Matić, N.; Bergman, T.; Gronlund, H.; van Hage, M. Dog Saliva - an Important Source of Dog Allergens. Allergy 2013, 68 (5), 585–592. https://doi.org/10.1111/all.12130",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3488"
}

Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, N., Bergman, T., Gronlund, H.,& van Hage, M.. (2013). Supplementary data for article: Polović, N.; Waden, K.; Binnmyr, J.; Hamsten, C.; Gronneberg, R.; Palmberg, C.; Milčić-Matić, N.; Bergman, T.; Gronlund, H.; van Hage, M. Dog Saliva - an Important Source of Dog Allergens. Allergy 2013, 68 (5), 585–592. https://doi.org/10.1111/all.12130. in Allergy
Wiley-Blackwell, Hoboken..
https://hdl.handle.net/21.15107/rcub_cherry_3488

Polović N, Waden K, Binnmyr J, Hamsten C, Gronneberg R, Palmberg C, Milčić-Matić N, Bergman T, Gronlund H, van Hage M. Supplementary data for article: Polović, N.; Waden, K.; Binnmyr, J.; Hamsten, C.; Gronneberg, R.; Palmberg, C.; Milčić-Matić, N.; Bergman, T.; Gronlund, H.; van Hage, M. Dog Saliva - an Important Source of Dog Allergens. Allergy 2013, 68 (5), 585–592. https://doi.org/10.1111/all.12130. in Allergy. 2013;.
https://hdl.handle.net/21.15107/rcub_cherry_3488 .

Polović, Natalija, Waden, Konrad, Binnmyr, J., Hamsten, Carl, Gronneberg, R., Palmberg, C., Milčić-Matić, Natalija, Bergman, T., Gronlund, Hans, van Hage, Marianne, "Supplementary data for article: Polović, N.; Waden, K.; Binnmyr, J.; Hamsten, C.; Gronneberg, R.; Palmberg, C.; Milčić-Matić, N.; Bergman, T.; Gronlund, H.; van Hage, M. Dog Saliva - an Important Source of Dog Allergens. Allergy 2013, 68 (5), 585–592. https://doi.org/10.1111/all.12130" in Allergy (2013),
https://hdl.handle.net/21.15107/rcub_cherry_3488 .

TY  - JOUR
AU  - Codina, R.
AU  - van Hage, Marianne
AU  - Polović, Natalija
AU  - Wadén, K.
AU  - Binnmyr, J.
AU  - Hamsten, Carl
AU  - Grönneberg, R.
AU  - Palmberg, C.
AU  - Milčić-Matić, Natalija
AU  - Bergman, T.
AU  - Grönlund, H.
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/121
T2  - Allergy
T1  - Diversity of allergens contained in dog saliva
VL  - 68
IS  - 11
SP  - 1484
EP  - 1485
DO  - 10.1111/all.12264
ER  - 

@article{
author = "Codina, R. and van Hage, Marianne and Polović, Natalija and Wadén, K. and Binnmyr, J. and Hamsten, Carl and Grönneberg, R. and Palmberg, C. and Milčić-Matić, Natalija and Bergman, T. and Grönlund, H.",
year = "2013",
journal = "Allergy",
title = "Diversity of allergens contained in dog saliva",
volume = "68",
number = "11",
pages = "1484-1485",
doi = "10.1111/all.12264"
}

Codina, R., van Hage, M., Polović, N., Wadén, K., Binnmyr, J., Hamsten, C., Grönneberg, R., Palmberg, C., Milčić-Matić, N., Bergman, T.,& Grönlund, H.. (2013). Diversity of allergens contained in dog saliva. in Allergy, 68(11), 1484-1485.
https://doi.org/10.1111/all.12264

Codina R, van Hage M, Polović N, Wadén K, Binnmyr J, Hamsten C, Grönneberg R, Palmberg C, Milčić-Matić N, Bergman T, Grönlund H. Diversity of allergens contained in dog saliva. in Allergy. 2013;68(11):1484-1485.
doi:10.1111/all.12264 .

Codina, R., van Hage, Marianne, Polović, Natalija, Wadén, K., Binnmyr, J., Hamsten, Carl, Grönneberg, R., Palmberg, C., Milčić-Matić, Natalija, Bergman, T., Grönlund, H., "Diversity of allergens contained in dog saliva" in Allergy, 68, no. 11 (2013):1484-1485,
https://doi.org/10.1111/all.12264 . .

TY  - CONF
AU  - Mihajlović, L.
AU  - Ćirković-Veličković, Tanja
AU  - Jadranin, Milka
AU  - Burazer, Lidija M.
AU  - Milčić-Matić, Natalija
PY  - 2010
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1469
PB  - Wiley-Blackwell, Hoboken
C3  - Allergy
T1  - The role of phenolic components of short ragweed pollen in igE binding and immune response to allergens
VL  - 65
SP  - 331
EP  - 331
UR  - https://hdl.handle.net/21.15107/rcub_cherry_1469
ER  - 

@conference{
author = "Mihajlović, L. and Ćirković-Veličković, Tanja and Jadranin, Milka and Burazer, Lidija M. and Milčić-Matić, Natalija",
year = "2010",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "The role of phenolic components of short ragweed pollen in igE binding and immune response to allergens",
volume = "65",
pages = "331-331",
url = "https://hdl.handle.net/21.15107/rcub_cherry_1469"
}

Mihajlović, L., Ćirković-Veličković, T., Jadranin, M., Burazer, L. M.,& Milčić-Matić, N.. (2010). The role of phenolic components of short ragweed pollen in igE binding and immune response to allergens. in Allergy
Wiley-Blackwell, Hoboken., 65, 331-331.
https://hdl.handle.net/21.15107/rcub_cherry_1469

Mihajlović L, Ćirković-Veličković T, Jadranin M, Burazer LM, Milčić-Matić N. The role of phenolic components of short ragweed pollen in igE binding and immune response to allergens. in Allergy. 2010;65:331-331.
https://hdl.handle.net/21.15107/rcub_cherry_1469 .

Mihajlović, L., Ćirković-Veličković, Tanja, Jadranin, Milka, Burazer, Lidija M., Milčić-Matić, Natalija, "The role of phenolic components of short ragweed pollen in igE binding and immune response to allergens" in Allergy, 65 (2010):331-331,
https://hdl.handle.net/21.15107/rcub_cherry_1469 .

TY  - CONF
AU  - Polović, Natalija
AU  - Bergman, T.
AU  - Milčić-Matić, Natalija
AU  - Gronlund, Hans
AU  - van Hage, Marianne
PY  - 2010
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1464
PB  - Wiley-Blackwell, Hoboken
C3  - Allergy
T1  - Dog saliva - a source of dog allergens
VL  - 65
SP  - 105
EP  - 105
UR  - https://hdl.handle.net/21.15107/rcub_cherry_1464
ER  - 

@conference{
author = "Polović, Natalija and Bergman, T. and Milčić-Matić, Natalija and Gronlund, Hans and van Hage, Marianne",
year = "2010",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "Dog saliva - a source of dog allergens",
volume = "65",
pages = "105-105",
url = "https://hdl.handle.net/21.15107/rcub_cherry_1464"
}

Polović, N., Bergman, T., Milčić-Matić, N., Gronlund, H.,& van Hage, M.. (2010). Dog saliva - a source of dog allergens. in Allergy
Wiley-Blackwell, Hoboken., 65, 105-105.
https://hdl.handle.net/21.15107/rcub_cherry_1464

Polović N, Bergman T, Milčić-Matić N, Gronlund H, van Hage M. Dog saliva - a source of dog allergens. in Allergy. 2010;65:105-105.
https://hdl.handle.net/21.15107/rcub_cherry_1464 .

Polović, Natalija, Bergman, T., Milčić-Matić, Natalija, Gronlund, Hans, van Hage, Marianne, "Dog saliva - a source of dog allergens" in Allergy, 65 (2010):105-105,
https://hdl.handle.net/21.15107/rcub_cherry_1464 .

TY  - JOUR
AU  - Perovic, I.
AU  - Milovanovic, M.
AU  - Stanić, Dragana
AU  - Burazer, Lidija M.
AU  - Petrović, D.
AU  - Milčić-Matić, Natalija
AU  - Gafvelin, Guro
AU  - van Hage, Marianne
AU  - Jankov, Ratko M.
AU  - Ćirković-Veličković, Tanja
PY  - 2009
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/600
AB  - Treating allergies with modified allergens is an approach to make the treatment safer and more efficient. Art v 1 is the most prominent allergen of mugwort pollen and a significant cause of hayfever around Europe. The aim of this study was to reduce the allergenicity of Art v 1 by acetylation, and to investigate the capacity of the modified protein to generate blocking antibodies. The reduction of allergenicity of Art v 1 following acetylation was monitored by immunoblot, ELISA inhibition using a pool of sera from mugwort pollen allergic patients, basophil activation assay and by skin prick testing of mugwort-allergic patients. Rabbits were immunized against Art v 1 and acetylated Art v 1 (acArt v 1) and the rabbit antisera were tested for their capacity to block human IgE binding in ELISA. Human T cell proliferation against Art v 1 and acArt v 1 was examined in peripheral blood mononuclear cells (PBMCs) of mugwort pollen allergic patients and cytokine release in PBMC cultures was monitored. Acetylation of Art v 1 gave a derivative of reduced allergenicity in the in vitro and ex vivo tests applied. The skin test reactivity to acArt v 1 was significantly reduced in 19 patients when compared with the reactivity to Art v 1. Rabbit antibodies to acArt v 1 and Art v 1 showed similar capacity to block human IgE binding to Art v 1 in inhibition ELISA. Both proteins were able to induce proliferation of PBMCs and CD3/CD4(+) cells of mugwort-allergic patients. Release of IL-5 was significantly reduced in cultures stimulated with acArt v 1. Art v 1 modified by acetylation had a significantly reduced allergenicity in vitro and in vivo, while its immunogenicity was retained. Modification of allergens by acetylation could be a new strategy for allergen-specific immunotherapy. Cite this as: I. Perovic, M. Milovanovic, D. Stanic, L. Burazer, D. Petrovic, N. Milcic-Matic, G. Gafvelin, M. van Hage, R. Jankov and T. Cirkovic Velickovic, Clinical and Experimental Allergy, 2009 (39) 435-446.
PB  - Wiley-Blackwell Publishing, Inc, Malden
T2  - Clinical and Experimental Allergy
T1  - Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation
VL  - 39
IS  - 3
SP  - 435
EP  - 446
DO  - 10.1111/j.1365-2222.2008.03158.x
ER  - 

@article{
author = "Perovic, I. and Milovanovic, M. and Stanić, Dragana and Burazer, Lidija M. and Petrović, D. and Milčić-Matić, Natalija and Gafvelin, Guro and van Hage, Marianne and Jankov, Ratko M. and Ćirković-Veličković, Tanja",
year = "2009",
abstract = "Treating allergies with modified allergens is an approach to make the treatment safer and more efficient. Art v 1 is the most prominent allergen of mugwort pollen and a significant cause of hayfever around Europe. The aim of this study was to reduce the allergenicity of Art v 1 by acetylation, and to investigate the capacity of the modified protein to generate blocking antibodies. The reduction of allergenicity of Art v 1 following acetylation was monitored by immunoblot, ELISA inhibition using a pool of sera from mugwort pollen allergic patients, basophil activation assay and by skin prick testing of mugwort-allergic patients. Rabbits were immunized against Art v 1 and acetylated Art v 1 (acArt v 1) and the rabbit antisera were tested for their capacity to block human IgE binding in ELISA. Human T cell proliferation against Art v 1 and acArt v 1 was examined in peripheral blood mononuclear cells (PBMCs) of mugwort pollen allergic patients and cytokine release in PBMC cultures was monitored. Acetylation of Art v 1 gave a derivative of reduced allergenicity in the in vitro and ex vivo tests applied. The skin test reactivity to acArt v 1 was significantly reduced in 19 patients when compared with the reactivity to Art v 1. Rabbit antibodies to acArt v 1 and Art v 1 showed similar capacity to block human IgE binding to Art v 1 in inhibition ELISA. Both proteins were able to induce proliferation of PBMCs and CD3/CD4(+) cells of mugwort-allergic patients. Release of IL-5 was significantly reduced in cultures stimulated with acArt v 1. Art v 1 modified by acetylation had a significantly reduced allergenicity in vitro and in vivo, while its immunogenicity was retained. Modification of allergens by acetylation could be a new strategy for allergen-specific immunotherapy. Cite this as: I. Perovic, M. Milovanovic, D. Stanic, L. Burazer, D. Petrovic, N. Milcic-Matic, G. Gafvelin, M. van Hage, R. Jankov and T. Cirkovic Velickovic, Clinical and Experimental Allergy, 2009 (39) 435-446.",
publisher = "Wiley-Blackwell Publishing, Inc, Malden",
journal = "Clinical and Experimental Allergy",
title = "Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation",
volume = "39",
number = "3",
pages = "435-446",
doi = "10.1111/j.1365-2222.2008.03158.x"
}

Perovic, I., Milovanovic, M., Stanić, D., Burazer, L. M., Petrović, D., Milčić-Matić, N., Gafvelin, G., van Hage, M., Jankov, R. M.,& Ćirković-Veličković, T.. (2009). Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation. in Clinical and Experimental Allergy
Wiley-Blackwell Publishing, Inc, Malden., 39(3), 435-446.
https://doi.org/10.1111/j.1365-2222.2008.03158.x

Perovic I, Milovanovic M, Stanić D, Burazer LM, Petrović D, Milčić-Matić N, Gafvelin G, van Hage M, Jankov RM, Ćirković-Veličković T. Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation. in Clinical and Experimental Allergy. 2009;39(3):435-446.
doi:10.1111/j.1365-2222.2008.03158.x .

Perovic, I., Milovanovic, M., Stanić, Dragana, Burazer, Lidija M., Petrović, D., Milčić-Matić, Natalija, Gafvelin, Guro, van Hage, Marianne, Jankov, Ratko M., Ćirković-Veličković, Tanja, "Allergenicity and immunogenicity of the major mugwort pollen allergen Art v 1 chemically modified by acetylation" in Clinical and Experimental Allergy, 39, no. 3 (2009):435-446,
https://doi.org/10.1111/j.1365-2222.2008.03158.x . .