TY  - JOUR
AU  - Nikolić, Aleksandra
AU  - Mark, Kevin J.
AU  - Dragičević, Sandra
AU  - Babić, Tamara
AU  - Milošević, Katarina
AU  - Nestorović, Branimir
AU  - Beškoski, Vladimir
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6285
AB  - Background: Leukocytes are key cellular effectors of inflammation in asthma and understandingtheir function in this disease is of crucial importance. Blood leukocytes reflect the actions oftheir counterparts in the lungs and they can be obtained through minimal invasive procedures as partof the peripheral blood.Objective: The aim of the study was to identify proteins in blood leukocyte proteomes that respond toex vivo treatment by prednisone in order to pinpoint candidates for predictive biomarkers in corticosteroid-responsive asthma.Methods: The study included five children diagnosed with asthma and five healthy children. After theex vivo treatment of blood samples with prednisone, lysis of erythrocytes was performed and proteinswere extracted from the remaining leukocytes by ultrasonic disintegration. Protein extracts were analyzedby reversed phase nano-liquidchromatography–tandem mass spectrometry (LC–MS/MS).Results: The stimulation of asthmatics' leukocytes with prednisone has led to an increase in the levelsof FYB (fold change 3.4) and LYZ (fold change 2.2) with a statistical significance of p
T2  - Current Proteomics
T1  - Pilot Study of the Ex Vivo Blood Leukocytes’ Proteomic Response to Prednisone Stimulation in Corticosteroid-responsive Asthma
VL  - 20
IS  - 1
SP  - 12
EP  - 18
DO  - 10.2174/1570164620666230220112500
ER  - 

@article{
author = "Nikolić, Aleksandra and Mark, Kevin J. and Dragičević, Sandra and Babić, Tamara and Milošević, Katarina and Nestorović, Branimir and Beškoski, Vladimir",
year = "2023",
abstract = "Background: Leukocytes are key cellular effectors of inflammation in asthma and understandingtheir function in this disease is of crucial importance. Blood leukocytes reflect the actions oftheir counterparts in the lungs and they can be obtained through minimal invasive procedures as partof the peripheral blood.Objective: The aim of the study was to identify proteins in blood leukocyte proteomes that respond toex vivo treatment by prednisone in order to pinpoint candidates for predictive biomarkers in corticosteroid-responsive asthma.Methods: The study included five children diagnosed with asthma and five healthy children. After theex vivo treatment of blood samples with prednisone, lysis of erythrocytes was performed and proteinswere extracted from the remaining leukocytes by ultrasonic disintegration. Protein extracts were analyzedby reversed phase nano-liquidchromatography–tandem mass spectrometry (LC–MS/MS).Results: The stimulation of asthmatics' leukocytes with prednisone has led to an increase in the levelsof FYB (fold change 3.4) and LYZ (fold change 2.2) with a statistical significance of p",
journal = "Current Proteomics",
title = "Pilot Study of the Ex Vivo Blood Leukocytes’ Proteomic Response to Prednisone Stimulation in Corticosteroid-responsive Asthma",
volume = "20",
number = "1",
pages = "12-18",
doi = "10.2174/1570164620666230220112500"
}

Nikolić, A., Mark, K. J., Dragičević, S., Babić, T., Milošević, K., Nestorović, B.,& Beškoski, V.. (2023). Pilot Study of the Ex Vivo Blood Leukocytes’ Proteomic Response to Prednisone Stimulation in Corticosteroid-responsive Asthma. in Current Proteomics, 20(1), 12-18.
https://doi.org/10.2174/1570164620666230220112500

Nikolić A, Mark KJ, Dragičević S, Babić T, Milošević K, Nestorović B, Beškoski V. Pilot Study of the Ex Vivo Blood Leukocytes’ Proteomic Response to Prednisone Stimulation in Corticosteroid-responsive Asthma. in Current Proteomics. 2023;20(1):12-18.
doi:10.2174/1570164620666230220112500 .

Nikolić, Aleksandra, Mark, Kevin J., Dragičević, Sandra, Babić, Tamara, Milošević, Katarina, Nestorović, Branimir, Beškoski, Vladimir, "Pilot Study of the Ex Vivo Blood Leukocytes’ Proteomic Response to Prednisone Stimulation in Corticosteroid-responsive Asthma" in Current Proteomics, 20, no. 1 (2023):12-18,
https://doi.org/10.2174/1570164620666230220112500 . .

TY  - THES
AU  - Nikolić, Aleksandra
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5568
AB  - Shizofrenija je široko rasprostranjena mentalna bolest koja dovodi do različitih funkcionalnih oštećenja i prerane smrti. Uzroci nastanka ovog oboljenja još uvek nisu sa sigurnošću utvrđeni i objašnjeni, pretpostavlja se da je shizofrenija uzrokovana kombinacijom genetike, uslova odrastanja i uticaja okoline. Psihijatrijska dijagnoza se zasniva na kliničkoj fenomenologiji i bazira se na proceni psihijatra na osnovu anamneze i informacija dobivenim od staratelja obolelih. Za razvoj instrumentalne metode za dijagnostiku ovih bolesti, zasnovane na analizama krvi, neophodno je uspostaviti univerzalni set biomarkera. Cilj ovog rada je ispitivanje sadržaja metabolita u uzorcima seruma obolelih pacijenata i kontrolnih uzoraka seruma zdravih osoba, tehnikom GC-MS. Analizirane su razlike u sadržaju metabolita između obolelih i zdravih. Utvrđeno je prisustvo 51 metabolita u uzorcima seruma, a poređenjem sadržaja metabolita u serumima obolelih sa sadržajem metabolita u serumu zdravih individua (kontrolni uzorci), utvrđeno je prisustvo 22 potencijalna biomarkera za shizofreniju.
T1  - Ispitivanje metabolita u serumu obolelih od shizofrenije GC/MS analizom
SP  - 1
EP  - 29
UR  - https://hdl.handle.net/21.15107/rcub_cherry_5568
ER  - 

@mastersthesis{
author = "Nikolić, Aleksandra",
year = "2022",
abstract = "Shizofrenija je široko rasprostranjena mentalna bolest koja dovodi do različitih funkcionalnih oštećenja i prerane smrti. Uzroci nastanka ovog oboljenja još uvek nisu sa sigurnošću utvrđeni i objašnjeni, pretpostavlja se da je shizofrenija uzrokovana kombinacijom genetike, uslova odrastanja i uticaja okoline. Psihijatrijska dijagnoza se zasniva na kliničkoj fenomenologiji i bazira se na proceni psihijatra na osnovu anamneze i informacija dobivenim od staratelja obolelih. Za razvoj instrumentalne metode za dijagnostiku ovih bolesti, zasnovane na analizama krvi, neophodno je uspostaviti univerzalni set biomarkera. Cilj ovog rada je ispitivanje sadržaja metabolita u uzorcima seruma obolelih pacijenata i kontrolnih uzoraka seruma zdravih osoba, tehnikom GC-MS. Analizirane su razlike u sadržaju metabolita između obolelih i zdravih. Utvrđeno je prisustvo 51 metabolita u uzorcima seruma, a poređenjem sadržaja metabolita u serumima obolelih sa sadržajem metabolita u serumu zdravih individua (kontrolni uzorci), utvrđeno je prisustvo 22 potencijalna biomarkera za shizofreniju.",
title = "Ispitivanje metabolita u serumu obolelih od shizofrenije GC/MS analizom",
pages = "1-29",
url = "https://hdl.handle.net/21.15107/rcub_cherry_5568"
}

Nikolić, A.. (2022). Ispitivanje metabolita u serumu obolelih od shizofrenije GC/MS analizom. , 1-29.
https://hdl.handle.net/21.15107/rcub_cherry_5568

Nikolić A. Ispitivanje metabolita u serumu obolelih od shizofrenije GC/MS analizom. 2022;:1-29.
https://hdl.handle.net/21.15107/rcub_cherry_5568 .

Nikolić, Aleksandra, "Ispitivanje metabolita u serumu obolelih od shizofrenije GC/MS analizom" (2022):1-29,
https://hdl.handle.net/21.15107/rcub_cherry_5568 .

TY  - JOUR
AU  - Milenković, Ivana
AU  - Radotić, Ksenija
AU  - Despotović, Jovana
AU  - Lončarević, Branka D.
AU  - Lješević, Marija
AU  - Spasić, Slađana Z.
AU  - Nikolić, Aleksandra
AU  - Beškoski, Vladimir
PY  - 2021
UR  - https://www.sciencedirect.com/science/article/pii/S0166445X21001260
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/4566
AB  - Cerium oxide nanoparticles (nCeO2) have widespread applications, but they can be hazardous to the environment. Some reports indicate the toxic effect of nCeO2 on tested animals, but literature data are mainly contradictory. Coating of nCeO2 can improve their suspension stability and change their interaction with the environment, which can consequently decrease their toxic effects. Herein, the exopolysaccharides levan and pullulan, due to their high water solubility, biocompatibility, and ability to form film, were used to coat nCeO2. Additionally, the monosaccharide glucose was used, since it is a common material for nanoparticle coating. This is the first study investigating the impact of carbohydrate-coated nCeO2 in comparison to uncoated nCeO2 using different model organisms. The aim of this study was to test the acute toxicity of carbohydrate-coated nCeO2 on the bacterium Vibrio fischeri NRRL B-11177, the crustacean Daphnia magna, and zebrafish Danio rerio. The second aim was to investigate the effects of nCeO2 on respiration in Daphnia magna which was performed for the first time. Finally, it was important to see the relation between Ce bioaccumulation in Daphnia magna and Danio rerio and other investigated parameters. Our results revealed that the coating decreased the toxicity of nCeO2 on Vibrio fischeri. The coating of nCeO2 did not affect the nanoparticles’ accumulation/adsorption or mortality in Daphnia magna or Danio rerio. Monitoring of respiration in Daphnia magna revealed changes in CO2 production after exposure to coated nCeO2, while the crustacean's O2 consumption was not affected by any of the coated nCeO2. In summary, this study revealed that, at 200 mg L−1, uncoated and carbohydrate-coated nCeO2 are not toxic for the tested organisms, however, the CO2 production in Daphnia magna is different when they are treated with coated and uncoated nCeO2. The highest production was in glucose and levan-coated nCeO2 according to their highest suspension stability. Daphnia magna (D. magna), Danio rerio (D. rerio), Vibrio fischeri (V. fischeri)
PB  - Elsevier
T2  - Aquatic Toxicology
T1  - Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio
VL  - 236
SP  - 105867
DO  - 10.1016/j.aquatox.2021.105867
ER  - 

@article{
author = "Milenković, Ivana and Radotić, Ksenija and Despotović, Jovana and Lončarević, Branka D. and Lješević, Marija and Spasić, Slađana Z. and Nikolić, Aleksandra and Beškoski, Vladimir",
year = "2021",
abstract = "Cerium oxide nanoparticles (nCeO2) have widespread applications, but they can be hazardous to the environment. Some reports indicate the toxic effect of nCeO2 on tested animals, but literature data are mainly contradictory. Coating of nCeO2 can improve their suspension stability and change their interaction with the environment, which can consequently decrease their toxic effects. Herein, the exopolysaccharides levan and pullulan, due to their high water solubility, biocompatibility, and ability to form film, were used to coat nCeO2. Additionally, the monosaccharide glucose was used, since it is a common material for nanoparticle coating. This is the first study investigating the impact of carbohydrate-coated nCeO2 in comparison to uncoated nCeO2 using different model organisms. The aim of this study was to test the acute toxicity of carbohydrate-coated nCeO2 on the bacterium Vibrio fischeri NRRL B-11177, the crustacean Daphnia magna, and zebrafish Danio rerio. The second aim was to investigate the effects of nCeO2 on respiration in Daphnia magna which was performed for the first time. Finally, it was important to see the relation between Ce bioaccumulation in Daphnia magna and Danio rerio and other investigated parameters. Our results revealed that the coating decreased the toxicity of nCeO2 on Vibrio fischeri. The coating of nCeO2 did not affect the nanoparticles’ accumulation/adsorption or mortality in Daphnia magna or Danio rerio. Monitoring of respiration in Daphnia magna revealed changes in CO2 production after exposure to coated nCeO2, while the crustacean's O2 consumption was not affected by any of the coated nCeO2. In summary, this study revealed that, at 200 mg L−1, uncoated and carbohydrate-coated nCeO2 are not toxic for the tested organisms, however, the CO2 production in Daphnia magna is different when they are treated with coated and uncoated nCeO2. The highest production was in glucose and levan-coated nCeO2 according to their highest suspension stability. Daphnia magna (D. magna), Danio rerio (D. rerio), Vibrio fischeri (V. fischeri)",
publisher = "Elsevier",
journal = "Aquatic Toxicology",
title = "Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio",
volume = "236",
pages = "105867",
doi = "10.1016/j.aquatox.2021.105867"
}

Milenković, I., Radotić, K., Despotović, J., Lončarević, B. D., Lješević, M., Spasić, S. Z., Nikolić, A.,& Beškoski, V.. (2021). Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio. in Aquatic Toxicology
Elsevier., 236, 105867.
https://doi.org/10.1016/j.aquatox.2021.105867

Milenković I, Radotić K, Despotović J, Lončarević BD, Lješević M, Spasić SZ, Nikolić A, Beškoski V. Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio. in Aquatic Toxicology. 2021;236:105867.
doi:10.1016/j.aquatox.2021.105867 .

Milenković, Ivana, Radotić, Ksenija, Despotović, Jovana, Lončarević, Branka D., Lješević, Marija, Spasić, Slađana Z., Nikolić, Aleksandra, Beškoski, Vladimir, "Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio" in Aquatic Toxicology, 236 (2021):105867,
https://doi.org/10.1016/j.aquatox.2021.105867 . .