TY  - JOUR
AU  - Lopandić, Zorana
AU  - Protić-Rosić, Isidora
AU  - Todorović, Aleksandra
AU  - Glamočlija, Sofija
AU  - Gnjatović, Marija Lj.
AU  - Ćujic, Danica
AU  - Gavrović-Jankulović, Marija
PY  - 2021
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/4800
AB  - Diagnostic evaluation of specific antibodies against the SARS-CoV-2 virus is mainly based on spike (S) and nucleocapsid (N) proteins. Despite the critical functions in virus infection and contribution to the pattern of immunodominance in COVID-19, exploitation of the most abundant membrane (M) protein in the SARS-CoV-2 serology tests is minimal. This study investigated the recombinant M protein’s immunoreactivity with the sera from COVID-19 convalescents. In silico designed protein was created from the outer N-terminal part (19 aa) and internal C-terminal tail (101–222 aa) of the M protein (YP_009724393.1) and was recombinantly produced and purified. The designed M protein (16,498.74 Da, pI 8.79) revealed both IgM and IgG reactivity with serum samples from COVID-19 convalescents in Western blot. In ELISA, more than 93% (28/30) of COVID-19 sera were positive for IgM detection, and more than 96% (29/30) were positive for specific IgG detection to M protein. Based on the capacity to provoke an immune response and its strong antigenic properties, as shown here, and the fact that it is also involved in the virion entry into host cells, the M protein of the SARS-CoV-2 virus as a good antigen has the potential in diagnostic purposes and vaccine design.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein
VL  - 22
IS  - 9
SP  - 4951
DO  - 10.3390/ijms22094951
ER  - 

@article{
author = "Lopandić, Zorana and Protić-Rosić, Isidora and Todorović, Aleksandra and Glamočlija, Sofija and Gnjatović, Marija Lj. and Ćujic, Danica and Gavrović-Jankulović, Marija",
year = "2021",
abstract = "Diagnostic evaluation of specific antibodies against the SARS-CoV-2 virus is mainly based on spike (S) and nucleocapsid (N) proteins. Despite the critical functions in virus infection and contribution to the pattern of immunodominance in COVID-19, exploitation of the most abundant membrane (M) protein in the SARS-CoV-2 serology tests is minimal. This study investigated the recombinant M protein’s immunoreactivity with the sera from COVID-19 convalescents. In silico designed protein was created from the outer N-terminal part (19 aa) and internal C-terminal tail (101–222 aa) of the M protein (YP_009724393.1) and was recombinantly produced and purified. The designed M protein (16,498.74 Da, pI 8.79) revealed both IgM and IgG reactivity with serum samples from COVID-19 convalescents in Western blot. In ELISA, more than 93% (28/30) of COVID-19 sera were positive for IgM detection, and more than 96% (29/30) were positive for specific IgG detection to M protein. Based on the capacity to provoke an immune response and its strong antigenic properties, as shown here, and the fact that it is also involved in the virion entry into host cells, the M protein of the SARS-CoV-2 virus as a good antigen has the potential in diagnostic purposes and vaccine design.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein",
volume = "22",
number = "9",
pages = "4951",
doi = "10.3390/ijms22094951"
}

Lopandić, Z., Protić-Rosić, I., Todorović, A., Glamočlija, S., Gnjatović, M. Lj., Ćujic, D.,& Gavrović-Jankulović, M.. (2021). IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein. in International Journal of Molecular Sciences
MDPI., 22(9), 4951.
https://doi.org/10.3390/ijms22094951

Lopandić Z, Protić-Rosić I, Todorović A, Glamočlija S, Gnjatović ML, Ćujic D, Gavrović-Jankulović M. IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein. in International Journal of Molecular Sciences. 2021;22(9):4951.
doi:10.3390/ijms22094951 .

Lopandić, Zorana, Protić-Rosić, Isidora, Todorović, Aleksandra, Glamočlija, Sofija, Gnjatović, Marija Lj., Ćujic, Danica, Gavrović-Jankulović, Marija, "IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein" in International Journal of Molecular Sciences, 22, no. 9 (2021):4951,
https://doi.org/10.3390/ijms22094951 . .