TY  - JOUR
AU  - Milutinović, Milica
AU  - Nakarada, Đura
AU  - Božunović, Jelena
AU  - Todorović, Miloš
AU  - Gašić, Uroš M.
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Ivković, Đurđa
AU  - Savić, Jelena
AU  - Devrnja, Nina
AU  - Aničić, Neda
AU  - Banjanac, Tijana
AU  - Mojović, Miloš
AU  - Mišić, Danijela
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5896
AB  - The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.
PB  - MDPI
T2  - Antioxidants
T2  - Antioxidants
T1  - Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening
VL  - 12
IS  - 2
SP  - 346
DO  - 10.3390/antiox12020346
ER  - 

@article{
author = "Milutinović, Milica and Nakarada, Đura and Božunović, Jelena and Todorović, Miloš and Gašić, Uroš M. and Živković, Suzana and Skorić, Marijana and Ivković, Đurđa and Savić, Jelena and Devrnja, Nina and Aničić, Neda and Banjanac, Tijana and Mojović, Miloš and Mišić, Danijela",
year = "2023",
abstract = "The present study provides, for the first time, a physicochemical and biochemical characterization of the redox processes associated with the ripening of Solanum dulcamara L. (bittersweet) berries. Electron Paramagnetic Resonance Spectroscopy (EPRS) and Imaging (EPRI) measurements of reactive oxygen species (ROS) were performed in parallel with the tissue-specific metabolic profiling of major antioxidants and assessment of antioxidant enzymes activity. Fruit transition from the mature green (MG) to ripe red (RR) stage involved changes in the qualitative and quantitative content of antioxidants and the associated cellular oxidation and peroxidation processes. The skin of bittersweet berries, which was the major source of antioxidants, exhibited the highest antioxidant potential against DPPH radicals and nitroxyl spin probe 3CP. The efficient enzymatic antioxidant system played a critical protective role against the deleterious effects of progressive oxidative stress during ripening. Here, we present the EPRI methodology to assess the redox status of fruits and to discriminate between the redox states of different tissues. Interestingly, the intracellular reoxidation of cell-permeable nitroxide probe 3CP was observed for the first time in fruits or any other plant tissue, and its intensity is herein proposed as a reliable indicator of oxidative stress during ripening. The described noninvasive EPRI technique has the potential to have broader application in the study of redox processes associated with the development, senescence, and postharvest storage of fruits, as well as other circumstances in which oxidative stress is implicated.",
publisher = "MDPI",
journal = "Antioxidants, Antioxidants",
title = "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening",
volume = "12",
number = "2",
pages = "346",
doi = "10.3390/antiox12020346"
}

Milutinović, M., Nakarada, Đ., Božunović, J., Todorović, M., Gašić, U. M., Živković, S., Skorić, M., Ivković, Đ., Savić, J., Devrnja, N., Aničić, N., Banjanac, T., Mojović, M.,& Mišić, D.. (2023). Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants
MDPI., 12(2), 346.
https://doi.org/10.3390/antiox12020346

Milutinović M, Nakarada Đ, Božunović J, Todorović M, Gašić UM, Živković S, Skorić M, Ivković Đ, Savić J, Devrnja N, Aničić N, Banjanac T, Mojović M, Mišić D. Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening. in Antioxidants. 2023;12(2):346.
doi:10.3390/antiox12020346 .

Milutinović, Milica, Nakarada, Đura, Božunović, Jelena, Todorović, Miloš, Gašić, Uroš M., Živković, Suzana, Skorić, Marijana, Ivković, Đurđa, Savić, Jelena, Devrnja, Nina, Aničić, Neda, Banjanac, Tijana, Mojović, Miloš, Mišić, Danijela, "Solanum dulcamara L. Berries: A Convenient Model System to Study Redox Processes in Relation to Fruit Ripening" in Antioxidants, 12, no. 2 (2023):346,
https://doi.org/10.3390/antiox12020346 . .

TY  - JOUR
AU  - Ćirković, Dušica
AU  - Matijašević, Saša
AU  - Ćirković, Bratislav
AU  - Laketić, Darko
AU  - Jovanović, Zoran
AU  - Kostić, Boban
AU  - Bešlić, Zoran
AU  - Sredojević, Milica
AU  - Tešić, Živoslav Lj.
AU  - Banjanac, Tijana
AU  - Gašić, Uroš M.
PY  - 2022
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/5317
AB  - The variety Prokupac is the dominant variety in the vineyards of Southern Serbia, which
produces quality wines of characteristic and unique tastes. In the agroecological conditions of the
Prokuplje vine district, the influence of manual defoliation on the phenolic profile of the wine
produced from the variety Prokupac was examined. Four experimental treatments with different
timings of manual defoliation were applied: early defoliation—treatment I, early defoliation—
treatment II, late defoliation—treatment III and the control. The phenolic profile of the wine
was determined for the three treatments of defoliation and the control treatment. Additionally,
a multivariate analysis was applied on the obtained results, together with already published data
(grape seeds and skins phenolic profiles). Identification and quantification of the phenolic compounds was performed using ultra-high-performance liquid chromatography (UHPLC) with an ultraviolet multi-diode detector (DAD) and mass detector with three analyzers—triple quadrupole (QQQ). Based on the obtained results, it was determined that there are significant differences between the experimental treatments in the content of individual polyphenols, total polyphenols and the antioxidant capacity. Twenty (20) phenolic compounds were identified in the wine samples of the experimental treatments. Defoliation significantly affected the variations of the contents of
phenolic acids and flavonoids. In treatment III, the highest content of gallic acid was obtained,
while the treatments with early defoliation did not differ in relation to the control sample. Early
defoliation in treatments I and II had an effect on the phenolic composition of the wine by favoring
the accumulation of flavonol, while the content of hydroxycinnamic acid and total anthocyanins
(TAC) was higher in treatment III. The TAC increases with later defoliation. The wines obtained by
the defoliation treatments did not show higher antioxidant activity compared to the control sample. A principal component analysis resulted in clustering of the samples based on the phenolic components characteristic for each group of samples.
PB  - MDPI
T2  - Horticulturae
T1  - Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.)
VL  - 8
SP  - 296
DO  - 10.3390/horticulturae8040296
ER  - 

@article{
author = "Ćirković, Dušica and Matijašević, Saša and Ćirković, Bratislav and Laketić, Darko and Jovanović, Zoran and Kostić, Boban and Bešlić, Zoran and Sredojević, Milica and Tešić, Živoslav Lj. and Banjanac, Tijana and Gašić, Uroš M.",
year = "2022",
abstract = "The variety Prokupac is the dominant variety in the vineyards of Southern Serbia, which
produces quality wines of characteristic and unique tastes. In the agroecological conditions of the
Prokuplje vine district, the influence of manual defoliation on the phenolic profile of the wine
produced from the variety Prokupac was examined. Four experimental treatments with different
timings of manual defoliation were applied: early defoliation—treatment I, early defoliation—
treatment II, late defoliation—treatment III and the control. The phenolic profile of the wine
was determined for the three treatments of defoliation and the control treatment. Additionally,
a multivariate analysis was applied on the obtained results, together with already published data
(grape seeds and skins phenolic profiles). Identification and quantification of the phenolic compounds was performed using ultra-high-performance liquid chromatography (UHPLC) with an ultraviolet multi-diode detector (DAD) and mass detector with three analyzers—triple quadrupole (QQQ). Based on the obtained results, it was determined that there are significant differences between the experimental treatments in the content of individual polyphenols, total polyphenols and the antioxidant capacity. Twenty (20) phenolic compounds were identified in the wine samples of the experimental treatments. Defoliation significantly affected the variations of the contents of
phenolic acids and flavonoids. In treatment III, the highest content of gallic acid was obtained,
while the treatments with early defoliation did not differ in relation to the control sample. Early
defoliation in treatments I and II had an effect on the phenolic composition of the wine by favoring
the accumulation of flavonol, while the content of hydroxycinnamic acid and total anthocyanins
(TAC) was higher in treatment III. The TAC increases with later defoliation. The wines obtained by
the defoliation treatments did not show higher antioxidant activity compared to the control sample. A principal component analysis resulted in clustering of the samples based on the phenolic components characteristic for each group of samples.",
publisher = "MDPI",
journal = "Horticulturae",
title = "Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.)",
volume = "8",
pages = "296",
doi = "10.3390/horticulturae8040296"
}

Ćirković, D., Matijašević, S., Ćirković, B., Laketić, D., Jovanović, Z., Kostić, B., Bešlić, Z., Sredojević, M., Tešić, Ž. Lj., Banjanac, T.,& Gašić, U. M.. (2022). Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.). in Horticulturae
MDPI., 8, 296.
https://doi.org/10.3390/horticulturae8040296

Ćirković D, Matijašević S, Ćirković B, Laketić D, Jovanović Z, Kostić B, Bešlić Z, Sredojević M, Tešić ŽL, Banjanac T, Gašić UM. Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.). in Horticulturae. 2022;8:296.
doi:10.3390/horticulturae8040296 .

Ćirković, Dušica, Matijašević, Saša, Ćirković, Bratislav, Laketić, Darko, Jovanović, Zoran, Kostić, Boban, Bešlić, Zoran, Sredojević, Milica, Tešić, Živoslav Lj., Banjanac, Tijana, Gašić, Uroš M., "Influence of Different Defoliation Timings on Quality and Phenolic Composition of the Wines Produced from the Serbian Autochthonous Variety Prokupac (Vitis vinifera L.)" in Horticulturae, 8 (2022):296,
https://doi.org/10.3390/horticulturae8040296 . .

TY  - JOUR
AU  - Banjanac, Tijana
AU  - Dragičević, Milan
AU  - Šiler, Branislav
AU  - Gašić, Uroš M.
AU  - Bohanec, Borut
AU  - Nestorović Živković, Jasmina
AU  - Trifunović, Snežana S.
AU  - Mišić, Danijela
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2477
AB  - Species within the genus Centaurium readily hybridize and polyploid complexes are often seen in natural populations. We describe phytochemical profiles of newly discovered allohexaploid hybrid, here named Centaurium pannonicum, and its parental tetraploid species C. erythraea and rare C. littorale ssp. compressum. Our aim was to examine chemodiversity of these taxa in the area of Vojvodina (North Serbia) and to perform metabolomics search for chemical classifiers which would provide high resolution discrimination of parental and hybrid individuals. In sum, UHPLC-MS/MS Orbitrap metabolomics fingerprinting revealed seventy compounds in methanol extracts. Despite the lack of qualitative chemical novelty in hybrid plants, UHPLC-qqqMS targeted metabolomics approach, aimed at three secoiridoid compounds and seventeen phenolics, pointed to considerable differences in quantitative composition of these dominant compounds among the plant taxa studied. In addition to the difference in the ploidy levels, the hybrid taxon was well distinguished from both parental species based on metabolite profiles, and, for most individuals, positioned intermediately to the parental taxa in both PCA and hierarchical clustering. After optimizing and comparing several statistical learning methods, it was possible to narrow the number of taxonomic classifiers to five (three xanthones, one secoiridoid glycoside, and one phenolic acid), while increasing the differentiation resolution. The presented metabolomics approach will certainly, along with morphometrics and molecular genetics studies, have high impact on further elucidation of complex relationships among taxa within the genus Centaurium.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Phytochemistry
T1  - Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers
VL  - 140
SP  - 27
EP  - 44
DO  - 10.1016/j.phytochem.2017.04.005
ER  - 

@article{
author = "Banjanac, Tijana and Dragičević, Milan and Šiler, Branislav and Gašić, Uroš M. and Bohanec, Borut and Nestorović Živković, Jasmina and Trifunović, Snežana S. and Mišić, Danijela",
year = "2017",
abstract = "Species within the genus Centaurium readily hybridize and polyploid complexes are often seen in natural populations. We describe phytochemical profiles of newly discovered allohexaploid hybrid, here named Centaurium pannonicum, and its parental tetraploid species C. erythraea and rare C. littorale ssp. compressum. Our aim was to examine chemodiversity of these taxa in the area of Vojvodina (North Serbia) and to perform metabolomics search for chemical classifiers which would provide high resolution discrimination of parental and hybrid individuals. In sum, UHPLC-MS/MS Orbitrap metabolomics fingerprinting revealed seventy compounds in methanol extracts. Despite the lack of qualitative chemical novelty in hybrid plants, UHPLC-qqqMS targeted metabolomics approach, aimed at three secoiridoid compounds and seventeen phenolics, pointed to considerable differences in quantitative composition of these dominant compounds among the plant taxa studied. In addition to the difference in the ploidy levels, the hybrid taxon was well distinguished from both parental species based on metabolite profiles, and, for most individuals, positioned intermediately to the parental taxa in both PCA and hierarchical clustering. After optimizing and comparing several statistical learning methods, it was possible to narrow the number of taxonomic classifiers to five (three xanthones, one secoiridoid glycoside, and one phenolic acid), while increasing the differentiation resolution. The presented metabolomics approach will certainly, along with morphometrics and molecular genetics studies, have high impact on further elucidation of complex relationships among taxa within the genus Centaurium.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Phytochemistry",
title = "Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers",
volume = "140",
pages = "27-44",
doi = "10.1016/j.phytochem.2017.04.005"
}

Banjanac, T., Dragičević, M., Šiler, B., Gašić, U. M., Bohanec, B., Nestorović Živković, J., Trifunović, S. S.,& Mišić, D.. (2017). Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers. in Phytochemistry
Pergamon-Elsevier Science Ltd, Oxford., 140, 27-44.
https://doi.org/10.1016/j.phytochem.2017.04.005

Banjanac T, Dragičević M, Šiler B, Gašić UM, Bohanec B, Nestorović Živković J, Trifunović SS, Mišić D. Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers. in Phytochemistry. 2017;140:27-44.
doi:10.1016/j.phytochem.2017.04.005 .

Banjanac, Tijana, Dragičević, Milan, Šiler, Branislav, Gašić, Uroš M., Bohanec, Borut, Nestorović Živković, Jasmina, Trifunović, Snežana S., Mišić, Danijela, "Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers" in Phytochemistry, 140 (2017):27-44,
https://doi.org/10.1016/j.phytochem.2017.04.005 . .

TY  - DATA
AU  - Banjanac, Tijana
AU  - Dragičević, Milan
AU  - Šiler, Branislav
AU  - Gašić, Uroš M.
AU  - Bohanec, Borut
AU  - Nestorović Živković, Jasmina
AU  - Trifunović, Snežana S.
AU  - Mišić, Danijela
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3098
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Phytochemistry
T1  - Supplementary data for article: Banjanac, T.; Dragićević, M.; Šiler, B.; Gašić, U.; Bohanec, B.; Nestorović Živković, J.; Trifunović, S.; Mišić, D. Chemodiversity of Two Closely Related Tetraploid Centaurium Species and Their Hexaploid Hybrid: Metabolomic Search for High-Resolution Taxonomic Classifiers. Phytochemistry 2017, 140, 27–44. https://doi.org/10.1016/j.phytochem.2017.04.005
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3098
ER  - 

@misc{
author = "Banjanac, Tijana and Dragičević, Milan and Šiler, Branislav and Gašić, Uroš M. and Bohanec, Borut and Nestorović Živković, Jasmina and Trifunović, Snežana S. and Mišić, Danijela",
year = "2017",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Phytochemistry",
title = "Supplementary data for article: Banjanac, T.; Dragićević, M.; Šiler, B.; Gašić, U.; Bohanec, B.; Nestorović Živković, J.; Trifunović, S.; Mišić, D. Chemodiversity of Two Closely Related Tetraploid Centaurium Species and Their Hexaploid Hybrid: Metabolomic Search for High-Resolution Taxonomic Classifiers. Phytochemistry 2017, 140, 27–44. https://doi.org/10.1016/j.phytochem.2017.04.005",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3098"
}

Banjanac, T., Dragičević, M., Šiler, B., Gašić, U. M., Bohanec, B., Nestorović Živković, J., Trifunović, S. S.,& Mišić, D.. (2017). Supplementary data for article: Banjanac, T.; Dragićević, M.; Šiler, B.; Gašić, U.; Bohanec, B.; Nestorović Živković, J.; Trifunović, S.; Mišić, D. Chemodiversity of Two Closely Related Tetraploid Centaurium Species and Their Hexaploid Hybrid: Metabolomic Search for High-Resolution Taxonomic Classifiers. Phytochemistry 2017, 140, 27–44. https://doi.org/10.1016/j.phytochem.2017.04.005. in Phytochemistry
Pergamon-Elsevier Science Ltd, Oxford..
https://hdl.handle.net/21.15107/rcub_cherry_3098

Banjanac T, Dragičević M, Šiler B, Gašić UM, Bohanec B, Nestorović Živković J, Trifunović SS, Mišić D. Supplementary data for article: Banjanac, T.; Dragićević, M.; Šiler, B.; Gašić, U.; Bohanec, B.; Nestorović Živković, J.; Trifunović, S.; Mišić, D. Chemodiversity of Two Closely Related Tetraploid Centaurium Species and Their Hexaploid Hybrid: Metabolomic Search for High-Resolution Taxonomic Classifiers. Phytochemistry 2017, 140, 27–44. https://doi.org/10.1016/j.phytochem.2017.04.005. in Phytochemistry. 2017;.
https://hdl.handle.net/21.15107/rcub_cherry_3098 .

Banjanac, Tijana, Dragičević, Milan, Šiler, Branislav, Gašić, Uroš M., Bohanec, Borut, Nestorović Živković, Jasmina, Trifunović, Snežana S., Mišić, Danijela, "Supplementary data for article: Banjanac, T.; Dragićević, M.; Šiler, B.; Gašić, U.; Bohanec, B.; Nestorović Živković, J.; Trifunović, S.; Mišić, D. Chemodiversity of Two Closely Related Tetraploid Centaurium Species and Their Hexaploid Hybrid: Metabolomic Search for High-Resolution Taxonomic Classifiers. Phytochemistry 2017, 140, 27–44. https://doi.org/10.1016/j.phytochem.2017.04.005" in Phytochemistry (2017),
https://hdl.handle.net/21.15107/rcub_cherry_3098 .