TY  - JOUR
AU  - Vujčić, Miroslava
AU  - Tufegdžić, Srđan
AU  - Novaković, Irena T.
AU  - Djikanovic, Daniela
AU  - Gasic, Miroslav J.
AU  - Sladić, Dušan
PY  - 2013
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1484
AB  - The interactions of avarone, a quinone from the marine sponge Dysideaavara, and the methylamino derivatives of avarone (2), 3'-(methylamino)avarone (3) and 4'-(methylamino)avarone (4) with calf thymus DNA (CT-DNA) were studied. Agarose gel electrophoreticanalysis showed that binding of the quinones quenched fluorescence of ethidium bromide (EB). The extent of fluorescence quenching of intercalator EB by competitive displacement from EB-CT-DNA system and of groove binder Hoechst 33258 (H) from H-CT-DNA system with the quinones was analyzed by fluorescence spectroscopy. The obtained results demonstrated that the quinones reduced binding of both the intercalator EB and the minor groove binder H, indicating possible degradation of DNA. The substituent on the quinone moiety determined the extent of DNA damaging effect of the quinone, which was the most extensive with 3'-(methylamino)avarone and the least extensive with its regioisomer 4'-(methylamino)avarone. The results were confirmed by the observed hyperchromic effects in UV-visible spectra measured after interactions of the derivatives with CT-DNA.
PB  - Elsevier Science Bv, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Studies on the interactions of bioactive quinone avarone and its methylamino derivatives with calf thymus DNA
VL  - 62
SP  - 405
EP  - 410
DO  - 10.1016/j.ijbiomac.2013.09.013
ER  - 

@article{
author = "Vujčić, Miroslava and Tufegdžić, Srđan and Novaković, Irena T. and Djikanovic, Daniela and Gasic, Miroslav J. and Sladić, Dušan",
year = "2013",
abstract = "The interactions of avarone, a quinone from the marine sponge Dysideaavara, and the methylamino derivatives of avarone (2), 3'-(methylamino)avarone (3) and 4'-(methylamino)avarone (4) with calf thymus DNA (CT-DNA) were studied. Agarose gel electrophoreticanalysis showed that binding of the quinones quenched fluorescence of ethidium bromide (EB). The extent of fluorescence quenching of intercalator EB by competitive displacement from EB-CT-DNA system and of groove binder Hoechst 33258 (H) from H-CT-DNA system with the quinones was analyzed by fluorescence spectroscopy. The obtained results demonstrated that the quinones reduced binding of both the intercalator EB and the minor groove binder H, indicating possible degradation of DNA. The substituent on the quinone moiety determined the extent of DNA damaging effect of the quinone, which was the most extensive with 3'-(methylamino)avarone and the least extensive with its regioisomer 4'-(methylamino)avarone. The results were confirmed by the observed hyperchromic effects in UV-visible spectra measured after interactions of the derivatives with CT-DNA.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Studies on the interactions of bioactive quinone avarone and its methylamino derivatives with calf thymus DNA",
volume = "62",
pages = "405-410",
doi = "10.1016/j.ijbiomac.2013.09.013"
}

Vujčić, M., Tufegdžić, S., Novaković, I. T., Djikanovic, D., Gasic, M. J.,& Sladić, D.. (2013). Studies on the interactions of bioactive quinone avarone and its methylamino derivatives with calf thymus DNA. in International Journal of Biological Macromolecules
Elsevier Science Bv, Amsterdam., 62, 405-410.
https://doi.org/10.1016/j.ijbiomac.2013.09.013

Vujčić M, Tufegdžić S, Novaković IT, Djikanovic D, Gasic MJ, Sladić D. Studies on the interactions of bioactive quinone avarone and its methylamino derivatives with calf thymus DNA. in International Journal of Biological Macromolecules. 2013;62:405-410.
doi:10.1016/j.ijbiomac.2013.09.013 .

Vujčić, Miroslava, Tufegdžić, Srđan, Novaković, Irena T., Djikanovic, Daniela, Gasic, Miroslav J., Sladić, Dušan, "Studies on the interactions of bioactive quinone avarone and its methylamino derivatives with calf thymus DNA" in International Journal of Biological Macromolecules, 62 (2013):405-410,
https://doi.org/10.1016/j.ijbiomac.2013.09.013 . .

TY  - JOUR
AU  - Novaković, Irena T.
AU  - Anđelković, Uroš
AU  - Zlatović, Mario
AU  - Gasic, Miroslav J.
AU  - Sladić, Dušan
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1241
AB  - A conjugate of lysozyme with avarone, a bioactive sesquiterpene quinone of marine origin, and its three derivatives were synthesized. MALDI TOF mass spectral analysis and tryptic digestion showed that the only residue in lysozyme that was modified by all derivatives was lysine 97. The identity of the residue was in full correlation with the prediction obtained by molecular modeling. All bioconjugates preserved most of the enzymatic activity of lysozyme. The melting point of the conjugates was slightly increased in comparison to lysozyme, indicating a slight stabilization of structure. The antibacterial activity of all the conjugates to both Gram positive and Gram negative bacteria was stronger than the activity of either lysozyme or the quinones, the MIC values being in low micromolar range for some conjugates.
PB  - Amer Chemical Soc, Washington
T2  - Bioconjugate Chemistry
T1  - Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives
VL  - 23
IS  - 1
SP  - 57
EP  - 65
DO  - 10.1021/bc200330m
ER  - 

@article{
author = "Novaković, Irena T. and Anđelković, Uroš and Zlatović, Mario and Gasic, Miroslav J. and Sladić, Dušan",
year = "2012",
abstract = "A conjugate of lysozyme with avarone, a bioactive sesquiterpene quinone of marine origin, and its three derivatives were synthesized. MALDI TOF mass spectral analysis and tryptic digestion showed that the only residue in lysozyme that was modified by all derivatives was lysine 97. The identity of the residue was in full correlation with the prediction obtained by molecular modeling. All bioconjugates preserved most of the enzymatic activity of lysozyme. The melting point of the conjugates was slightly increased in comparison to lysozyme, indicating a slight stabilization of structure. The antibacterial activity of all the conjugates to both Gram positive and Gram negative bacteria was stronger than the activity of either lysozyme or the quinones, the MIC values being in low micromolar range for some conjugates.",
publisher = "Amer Chemical Soc, Washington",
journal = "Bioconjugate Chemistry",
title = "Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives",
volume = "23",
number = "1",
pages = "57-65",
doi = "10.1021/bc200330m"
}

Novaković, I. T., Anđelković, U., Zlatović, M., Gasic, M. J.,& Sladić, D.. (2012). Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives. in Bioconjugate Chemistry
Amer Chemical Soc, Washington., 23(1), 57-65.
https://doi.org/10.1021/bc200330m

Novaković IT, Anđelković U, Zlatović M, Gasic MJ, Sladić D. Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives. in Bioconjugate Chemistry. 2012;23(1):57-65.
doi:10.1021/bc200330m .

Novaković, Irena T., Anđelković, Uroš, Zlatović, Mario, Gasic, Miroslav J., Sladić, Dušan, "Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives" in Bioconjugate Chemistry, 23, no. 1 (2012):57-65,
https://doi.org/10.1021/bc200330m . .

TY  - JOUR
AU  - Novaković, Irena T.
AU  - Anđelković, Uroš
AU  - Zlatović, Mario
AU  - Gasic, Miroslav J.
AU  - Sladić, Dušan
PY  - 2012
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2786
AB  - A conjugate of lysozyme with avarone, a bioactive sesquiterpene quinone of marine origin, and its three derivatives were synthesized. MALDI TOF mass spectral analysis and tryptic digestion showed that the only residue in lysozyme that was modified by all derivatives was lysine 97. The identity of the residue was in full correlation with the prediction obtained by molecular modeling. All bioconjugates preserved most of the enzymatic activity of lysozyme. The melting point of the conjugates was slightly increased in comparison to lysozyme, indicating a slight stabilization of structure. The antibacterial activity of all the conjugates to both Gram positive and Gram negative bacteria was stronger than the activity of either lysozyme or the quinones, the MIC values being in low micromolar range for some conjugates.
PB  - Amer Chemical Soc, Washington
T2  - Bioconjugate Chemistry
T1  - Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives
VL  - 23
IS  - 1
SP  - 57
EP  - 65
DO  - 10.1021/bc200330m
ER  - 

@article{
author = "Novaković, Irena T. and Anđelković, Uroš and Zlatović, Mario and Gasic, Miroslav J. and Sladić, Dušan",
year = "2012",
abstract = "A conjugate of lysozyme with avarone, a bioactive sesquiterpene quinone of marine origin, and its three derivatives were synthesized. MALDI TOF mass spectral analysis and tryptic digestion showed that the only residue in lysozyme that was modified by all derivatives was lysine 97. The identity of the residue was in full correlation with the prediction obtained by molecular modeling. All bioconjugates preserved most of the enzymatic activity of lysozyme. The melting point of the conjugates was slightly increased in comparison to lysozyme, indicating a slight stabilization of structure. The antibacterial activity of all the conjugates to both Gram positive and Gram negative bacteria was stronger than the activity of either lysozyme or the quinones, the MIC values being in low micromolar range for some conjugates.",
publisher = "Amer Chemical Soc, Washington",
journal = "Bioconjugate Chemistry",
title = "Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives",
volume = "23",
number = "1",
pages = "57-65",
doi = "10.1021/bc200330m"
}

Novaković, I. T., Anđelković, U., Zlatović, M., Gasic, M. J.,& Sladić, D.. (2012). Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives. in Bioconjugate Chemistry
Amer Chemical Soc, Washington., 23(1), 57-65.
https://doi.org/10.1021/bc200330m

Novaković IT, Anđelković U, Zlatović M, Gasic MJ, Sladić D. Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives. in Bioconjugate Chemistry. 2012;23(1):57-65.
doi:10.1021/bc200330m .

Novaković, Irena T., Anđelković, Uroš, Zlatović, Mario, Gasic, Miroslav J., Sladić, Dušan, "Bioconjugate of Lysozyme and the Antibacterial Marine Sesquiterpene Quinone Avarone and Its Derivatives" in Bioconjugate Chemistry, 23, no. 1 (2012):57-65,
https://doi.org/10.1021/bc200330m . .

TY  - JOUR
AU  - Božić, Tatjana T.
AU  - Novaković, Irena T.
AU  - Gasic, Miroslav J.
AU  - Juranić, Zorica D.
AU  - Stanojković, Tatjana
AU  - Tufegdžić, Srđan
AU  - Kljajić, Zoran
AU  - Sladić, Dušan
PY  - 2010
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1057
AB  - Nine alkyl(aryl)thio derivatives of the marine sesquiterpene quinone avarone were synthesized by nucleophilic addition of thiols or thiophenol to avarone. In most cases only one regioisomer was obtained. Their cytotoxic activities, brine shrimp lethality and antibacterial activity were evaluated, as well as those of some previously synthesized avarone derivatives. Anti-HIV activity of two derivatives was tested. Electrochemical properties were determined for all the derivatives in Order to obtain more accurate information on structure-activity relationships. Most derivatives showed cytotoxic activity against tumor cell lines, with IC(50) values less than 10 mu M for some of them, in particular those with electron-donating substituents. The most active Compound was 4'-(methylamino)avarone, with IC(50) value of 2.4 mu M to melanoma Fem-X cells, and no cytotoxicity to normal lymphocytes. (C) 2009 Elsevier Masson SAS. All Fights reserved.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Paris
T2  - European Journal of Medicinal Chemistry
T1  - Synthesis and biological activity of derivatives of the marine quinone avarone
VL  - 45
IS  - 3
SP  - 923
EP  - 929
DO  - 10.1016/j.ejmech.2009.11.033
ER  - 

@article{
author = "Božić, Tatjana T. and Novaković, Irena T. and Gasic, Miroslav J. and Juranić, Zorica D. and Stanojković, Tatjana and Tufegdžić, Srđan and Kljajić, Zoran and Sladić, Dušan",
year = "2010",
abstract = "Nine alkyl(aryl)thio derivatives of the marine sesquiterpene quinone avarone were synthesized by nucleophilic addition of thiols or thiophenol to avarone. In most cases only one regioisomer was obtained. Their cytotoxic activities, brine shrimp lethality and antibacterial activity were evaluated, as well as those of some previously synthesized avarone derivatives. Anti-HIV activity of two derivatives was tested. Electrochemical properties were determined for all the derivatives in Order to obtain more accurate information on structure-activity relationships. Most derivatives showed cytotoxic activity against tumor cell lines, with IC(50) values less than 10 mu M for some of them, in particular those with electron-donating substituents. The most active Compound was 4'-(methylamino)avarone, with IC(50) value of 2.4 mu M to melanoma Fem-X cells, and no cytotoxicity to normal lymphocytes. (C) 2009 Elsevier Masson SAS. All Fights reserved.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Paris",
journal = "European Journal of Medicinal Chemistry",
title = "Synthesis and biological activity of derivatives of the marine quinone avarone",
volume = "45",
number = "3",
pages = "923-929",
doi = "10.1016/j.ejmech.2009.11.033"
}

Božić, T. T., Novaković, I. T., Gasic, M. J., Juranić, Z. D., Stanojković, T., Tufegdžić, S., Kljajić, Z.,& Sladić, D.. (2010). Synthesis and biological activity of derivatives of the marine quinone avarone. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Paris., 45(3), 923-929.
https://doi.org/10.1016/j.ejmech.2009.11.033

Božić TT, Novaković IT, Gasic MJ, Juranić ZD, Stanojković T, Tufegdžić S, Kljajić Z, Sladić D. Synthesis and biological activity of derivatives of the marine quinone avarone. in European Journal of Medicinal Chemistry. 2010;45(3):923-929.
doi:10.1016/j.ejmech.2009.11.033 .

Božić, Tatjana T., Novaković, Irena T., Gasic, Miroslav J., Juranić, Zorica D., Stanojković, Tatjana, Tufegdžić, Srđan, Kljajić, Zoran, Sladić, Dušan, "Synthesis and biological activity of derivatives of the marine quinone avarone" in European Journal of Medicinal Chemistry, 45, no. 3 (2010):923-929,
https://doi.org/10.1016/j.ejmech.2009.11.033 . .

TY  - JOUR
AU  - Milić, Dragana
AU  - Kop, Tatjana
AU  - Csanadi, Janos
AU  - Juranić, Zorica D.
AU  - Žižak, Željko S.
AU  - Gasic, Miroslav J.
AU  - Šolaja, Bogdan A.
PY  - 2009
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/1020
AB  - A simple approach to a stable steroidal estrone derived A.B-spiro system is reported. Treatment of estrone derived A-ring diepoxyalcohol with the Ac(2)O-TMSOTf system at the ambient temperature led to acetylation, while at the reflux temperature the acid-catalysed rearrangement took place affording the spiro-compound. Results of extensive in vitro and in vivo anticancer tests on the diepoxide, as well as preliminary data on the anti proliferative activity of the spiro-product against three cancer cell lines, are also presented. (C) 2009 Elsevier Inc. All rights reserved.
PB  - Elsevier Science Inc, New York
T2  - Steroids
T1  - Estrone derived steroidal diepoxide: Biologically active compound and precursor of a stable steroidal A,B-spiro system
VL  - 74
IS  - 12
SP  - 890
EP  - 895
DO  - 10.1016/j.steroids.2009.06.002
ER  - 

@article{
author = "Milić, Dragana and Kop, Tatjana and Csanadi, Janos and Juranić, Zorica D. and Žižak, Željko S. and Gasic, Miroslav J. and Šolaja, Bogdan A.",
year = "2009",
abstract = "A simple approach to a stable steroidal estrone derived A.B-spiro system is reported. Treatment of estrone derived A-ring diepoxyalcohol with the Ac(2)O-TMSOTf system at the ambient temperature led to acetylation, while at the reflux temperature the acid-catalysed rearrangement took place affording the spiro-compound. Results of extensive in vitro and in vivo anticancer tests on the diepoxide, as well as preliminary data on the anti proliferative activity of the spiro-product against three cancer cell lines, are also presented. (C) 2009 Elsevier Inc. All rights reserved.",
publisher = "Elsevier Science Inc, New York",
journal = "Steroids",
title = "Estrone derived steroidal diepoxide: Biologically active compound and precursor of a stable steroidal A,B-spiro system",
volume = "74",
number = "12",
pages = "890-895",
doi = "10.1016/j.steroids.2009.06.002"
}

Milić, D., Kop, T., Csanadi, J., Juranić, Z. D., Žižak, Ž. S., Gasic, M. J.,& Šolaja, B. A.. (2009). Estrone derived steroidal diepoxide: Biologically active compound and precursor of a stable steroidal A,B-spiro system. in Steroids
Elsevier Science Inc, New York., 74(12), 890-895.
https://doi.org/10.1016/j.steroids.2009.06.002

Milić D, Kop T, Csanadi J, Juranić ZD, Žižak ŽS, Gasic MJ, Šolaja BA. Estrone derived steroidal diepoxide: Biologically active compound and precursor of a stable steroidal A,B-spiro system. in Steroids. 2009;74(12):890-895.
doi:10.1016/j.steroids.2009.06.002 .

Milić, Dragana, Kop, Tatjana, Csanadi, Janos, Juranić, Zorica D., Žižak, Željko S., Gasic, Miroslav J., Šolaja, Bogdan A., "Estrone derived steroidal diepoxide: Biologically active compound and precursor of a stable steroidal A,B-spiro system" in Steroids, 74, no. 12 (2009):890-895,
https://doi.org/10.1016/j.steroids.2009.06.002 . .

TY  - JOUR
AU  - Tsoukatou, Maria
AU  - Marechal, Jean Philippe
AU  - Hellio, Claire
AU  - Novaković, Irena T.
AU  - Tufegdzie, Srdan
AU  - Sladić, Dušan
AU  - Gasic, Miroslav J.
AU  - Clare, Anthony S.
AU  - Vagias, Constantinos
AU  - Roussis, Vassilios
PY  - 2007
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/840
AB  - The sesquiterpene hydroquinone avarol (1) was isolated from the marine sponge Dysidea avara, whereas the corresponding quitione, avarone (2), was obtained by oxidation of avarol, and the significantly more lipophilic compounds [3 '-(P-chlorophenyl)avarone (3), 3 ',4 '-ethylenedithioavarone (4), 4 '-isopropylthioavarone (5), 4 '-tertbutylthioavarone (6), 4 '-propylthioavarone (7), 4 '-octylthioavarone (8)] were obtained by nucleophilic addition of thiols or p-chloroaniline to avarone. All these compounds were tested, at concentrations ranging from 0.5 to 50 mu g/mL, for their effect on the settlement of the cyprid stage of Balanus amphitrite, for toxicity to both nauplii and cyprids and for their growth inhibitory activity on marine bacteria (Cobetia marina, Marinobacterium stanieri, Vibrio fischeri and Pseudoalteromonas haloplanktis) and marine fungi (Halosphaeriopsis mediosetigera, Asteromyces cruciatus, Lulworthia uniseptata and Monodictys pelagica).
PB  - Mdpi Ag, Basel
T2  - Molecules
T1  - Evaluation of the activity of the sponge metabolites avarol and avarone and their synthetic derivatives against fouling micro- and macroorganisms
VL  - 12
IS  - 5
SP  - 1022
EP  - 1034
DO  - 10.3390/12051022
ER  - 

@article{
author = "Tsoukatou, Maria and Marechal, Jean Philippe and Hellio, Claire and Novaković, Irena T. and Tufegdzie, Srdan and Sladić, Dušan and Gasic, Miroslav J. and Clare, Anthony S. and Vagias, Constantinos and Roussis, Vassilios",
year = "2007",
abstract = "The sesquiterpene hydroquinone avarol (1) was isolated from the marine sponge Dysidea avara, whereas the corresponding quitione, avarone (2), was obtained by oxidation of avarol, and the significantly more lipophilic compounds [3 '-(P-chlorophenyl)avarone (3), 3 ',4 '-ethylenedithioavarone (4), 4 '-isopropylthioavarone (5), 4 '-tertbutylthioavarone (6), 4 '-propylthioavarone (7), 4 '-octylthioavarone (8)] were obtained by nucleophilic addition of thiols or p-chloroaniline to avarone. All these compounds were tested, at concentrations ranging from 0.5 to 50 mu g/mL, for their effect on the settlement of the cyprid stage of Balanus amphitrite, for toxicity to both nauplii and cyprids and for their growth inhibitory activity on marine bacteria (Cobetia marina, Marinobacterium stanieri, Vibrio fischeri and Pseudoalteromonas haloplanktis) and marine fungi (Halosphaeriopsis mediosetigera, Asteromyces cruciatus, Lulworthia uniseptata and Monodictys pelagica).",
publisher = "Mdpi Ag, Basel",
journal = "Molecules",
title = "Evaluation of the activity of the sponge metabolites avarol and avarone and their synthetic derivatives against fouling micro- and macroorganisms",
volume = "12",
number = "5",
pages = "1022-1034",
doi = "10.3390/12051022"
}

Tsoukatou, M., Marechal, J. P., Hellio, C., Novaković, I. T., Tufegdzie, S., Sladić, D., Gasic, M. J., Clare, A. S., Vagias, C.,& Roussis, V.. (2007). Evaluation of the activity of the sponge metabolites avarol and avarone and their synthetic derivatives against fouling micro- and macroorganisms. in Molecules
Mdpi Ag, Basel., 12(5), 1022-1034.
https://doi.org/10.3390/12051022

Tsoukatou M, Marechal JP, Hellio C, Novaković IT, Tufegdzie S, Sladić D, Gasic MJ, Clare AS, Vagias C, Roussis V. Evaluation of the activity of the sponge metabolites avarol and avarone and their synthetic derivatives against fouling micro- and macroorganisms. in Molecules. 2007;12(5):1022-1034.
doi:10.3390/12051022 .

Tsoukatou, Maria, Marechal, Jean Philippe, Hellio, Claire, Novaković, Irena T., Tufegdzie, Srdan, Sladić, Dušan, Gasic, Miroslav J., Clare, Anthony S., Vagias, Constantinos, Roussis, Vassilios, "Evaluation of the activity of the sponge metabolites avarol and avarone and their synthetic derivatives against fouling micro- and macroorganisms" in Molecules, 12, no. 5 (2007):1022-1034,
https://doi.org/10.3390/12051022 . .

TY  - JOUR
AU  - Pajic, Ivana
AU  - Vujčić, Zoran
AU  - Vujčić, Miroslava
AU  - Novaković, Irena T.
AU  - Sladić, Dušan
AU  - Gasic, Miroslav J.
PY  - 2007
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/906
AB  - The quinone avarone, isolated from the marine sponge Dysidea avara, possesses the ability to chemically modify proteins. In this work, modification of lectin isolated from the coral Gerardia savaglia by avarone was examined. The techniques used for studying the modification were: SDS PAGE, isoelectric focusing and hemagglutination testing. The results of the SDS PAGE indicate dimerization of the protein. A shift of the pI toward lower value occurs upon modification. The change of the hemagglutination activity of the protein confirms that chemical modification of G. savaglia lectin by avarone changes its ability to interact with the membrane of erythrocytes.
AB  - Avaron, hinon izolovan iz morskog sunđera Dysidea avara, poseduje sposobnost da hemijski modifikuje proteine. U ovom radu ispitivana je modifikacija lektina izolovanog iz korala Gerardia savaglia avaronom. Tehnike za praćenje hemijske modifikacije bile su: SDS PAGE, izoelektrično fokusiranje i hemaglutinacioni test. Rezultati SDS PAGE upućuju na dimerizaciju proteina. Dolazi do pomeranja pI vrednosti proteina. Promena hemaglutinacione aktivnosti G. savaglia lektina avaronom uticala je na njegovu sposobnost interakcije sa membranom eritrocita.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone
T1  - Hemijske modifikacije lektina morskog korala Gerardia savaglia morskim hinonom avaronom
VL  - 72
IS  - 12
SP  - 1271
EP  - 1274
DO  - 10.2298/JSC0712271P
ER  - 

@article{
author = "Pajic, Ivana and Vujčić, Zoran and Vujčić, Miroslava and Novaković, Irena T. and Sladić, Dušan and Gasic, Miroslav J.",
year = "2007",
abstract = "The quinone avarone, isolated from the marine sponge Dysidea avara, possesses the ability to chemically modify proteins. In this work, modification of lectin isolated from the coral Gerardia savaglia by avarone was examined. The techniques used for studying the modification were: SDS PAGE, isoelectric focusing and hemagglutination testing. The results of the SDS PAGE indicate dimerization of the protein. A shift of the pI toward lower value occurs upon modification. The change of the hemagglutination activity of the protein confirms that chemical modification of G. savaglia lectin by avarone changes its ability to interact with the membrane of erythrocytes., Avaron, hinon izolovan iz morskog sunđera Dysidea avara, poseduje sposobnost da hemijski modifikuje proteine. U ovom radu ispitivana je modifikacija lektina izolovanog iz korala Gerardia savaglia avaronom. Tehnike za praćenje hemijske modifikacije bile su: SDS PAGE, izoelektrično fokusiranje i hemaglutinacioni test. Rezultati SDS PAGE upućuju na dimerizaciju proteina. Dolazi do pomeranja pI vrednosti proteina. Promena hemaglutinacione aktivnosti G. savaglia lektina avaronom uticala je na njegovu sposobnost interakcije sa membranom eritrocita.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone, Hemijske modifikacije lektina morskog korala Gerardia savaglia morskim hinonom avaronom",
volume = "72",
number = "12",
pages = "1271-1274",
doi = "10.2298/JSC0712271P"
}

Pajic, I., Vujčić, Z., Vujčić, M., Novaković, I. T., Sladić, D.,& Gasic, M. J.. (2007). Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 72(12), 1271-1274.
https://doi.org/10.2298/JSC0712271P

Pajic I, Vujčić Z, Vujčić M, Novaković IT, Sladić D, Gasic MJ. Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone. in Journal of the Serbian Chemical Society. 2007;72(12):1271-1274.
doi:10.2298/JSC0712271P .

Pajic, Ivana, Vujčić, Zoran, Vujčić, Miroslava, Novaković, Irena T., Sladić, Dušan, Gasic, Miroslav J., "Chemical modification of the lectin of the marine coral Gerardia savaglia by marine quinone avarone" in Journal of the Serbian Chemical Society, 72, no. 12 (2007):1271-1274,
https://doi.org/10.2298/JSC0712271P . .

TY  - JOUR
AU  - Vujčić, Miroslava
AU  - Tufegdžić, Srđan
AU  - Vujčić, Zoran
AU  - Gasic, Miroslav J.
AU  - Sladić, Dušan
PY  - 2007
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/905
AB  - Changes in electrophoresis pattern after interaction of supercoiled plasmid pBR322 DNA with avarol was studied at a micromolar concentration of reactants under mild reaction conditions. Interactions of avarol with linear high-molecular CT-DNA at millimolar concentrations were analyzed by electrophoresis and UV spectrophotometry. It was observed that avarol is capable of quenching ethidium bromide fluorescence in DNA bands. An increase in the absorbance of DNA was detected. The results indicate the binding of avarol to DNA and/or modification of nucleotide bases.
AB  - Proučavane su promene elektroforetskog ponašanja DNA posle interakcija superhelikoidalnog plazmida pBR322 s avarolom pri mikromolarnim koncentracijama reaktanata pod blagim reakcionim uslovima. Interakcije avarola sa linearnom visokomolekulskom CT-DNA pri milimolarnim koncentracijama analizirane su elektroforezom i UV spektrofotometrijom. Uočeno je da je avarol u stanju da gasi fluorescenciju etidijum-bromida u trakama koje potiču od DNA. Detektovan je porast apsorbancije DNA. Rezultati ukazuju na vezivanje avarona za DNA i/ili modifikaciju nukleotidnih baza.
PB  - Serbian Chemical Soc, Belgrade
T2  - Journal of the Serbian Chemical Society
T1  - Interactions of the anti-tumor sesquiterpene hydroquinone avarol with DNA in vitro
T1  - Interakcije antitumorskog seskviterpenskog hidrohinona avarola sa DNA in vitro
VL  - 72
IS  - 12
SP  - 1265
EP  - 1269
DO  - 10.2298/JSC0712265V
ER  - 

@article{
author = "Vujčić, Miroslava and Tufegdžić, Srđan and Vujčić, Zoran and Gasic, Miroslav J. and Sladić, Dušan",
year = "2007",
abstract = "Changes in electrophoresis pattern after interaction of supercoiled plasmid pBR322 DNA with avarol was studied at a micromolar concentration of reactants under mild reaction conditions. Interactions of avarol with linear high-molecular CT-DNA at millimolar concentrations were analyzed by electrophoresis and UV spectrophotometry. It was observed that avarol is capable of quenching ethidium bromide fluorescence in DNA bands. An increase in the absorbance of DNA was detected. The results indicate the binding of avarol to DNA and/or modification of nucleotide bases., Proučavane su promene elektroforetskog ponašanja DNA posle interakcija superhelikoidalnog plazmida pBR322 s avarolom pri mikromolarnim koncentracijama reaktanata pod blagim reakcionim uslovima. Interakcije avarola sa linearnom visokomolekulskom CT-DNA pri milimolarnim koncentracijama analizirane su elektroforezom i UV spektrofotometrijom. Uočeno je da je avarol u stanju da gasi fluorescenciju etidijum-bromida u trakama koje potiču od DNA. Detektovan je porast apsorbancije DNA. Rezultati ukazuju na vezivanje avarona za DNA i/ili modifikaciju nukleotidnih baza.",
publisher = "Serbian Chemical Soc, Belgrade",
journal = "Journal of the Serbian Chemical Society",
title = "Interactions of the anti-tumor sesquiterpene hydroquinone avarol with DNA in vitro, Interakcije antitumorskog seskviterpenskog hidrohinona avarola sa DNA in vitro",
volume = "72",
number = "12",
pages = "1265-1269",
doi = "10.2298/JSC0712265V"
}

Vujčić, M., Tufegdžić, S., Vujčić, Z., Gasic, M. J.,& Sladić, D.. (2007). Interactions of the anti-tumor sesquiterpene hydroquinone avarol with DNA in vitro. in Journal of the Serbian Chemical Society
Serbian Chemical Soc, Belgrade., 72(12), 1265-1269.
https://doi.org/10.2298/JSC0712265V

Vujčić M, Tufegdžić S, Vujčić Z, Gasic MJ, Sladić D. Interactions of the anti-tumor sesquiterpene hydroquinone avarol with DNA in vitro. in Journal of the Serbian Chemical Society. 2007;72(12):1265-1269.
doi:10.2298/JSC0712265V .

Vujčić, Miroslava, Tufegdžić, Srđan, Vujčić, Zoran, Gasic, Miroslav J., Sladić, Dušan, "Interactions of the anti-tumor sesquiterpene hydroquinone avarol with DNA in vitro" in Journal of the Serbian Chemical Society, 72, no. 12 (2007):1265-1269,
https://doi.org/10.2298/JSC0712265V . .