TY  - JOUR
AU  - Jevtic, Bojan
AU  - Djedovic, Neda
AU  - Stanisavljevic, Suzana
AU  - Gašić, Uroš M.
AU  - Mišić, Danijela
AU  - Despotović, Jovana
AU  - Samardžić, Jelena
AU  - Miljković, Đorđe
AU  - Timotijević, Gordana
PY  - 2017
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2537
AB  - Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties. (C) 2017 Elsevier Ltd. All rights reserved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Functional Foods
T1  - Anti-encephalitogenic effects of cucumber leaf extract
VL  - 37
SP  - 249
EP  - 262
DO  - 10.1016/j.jff.2017.07.060
ER  - 

@article{
author = "Jevtic, Bojan and Djedovic, Neda and Stanisavljevic, Suzana and Gašić, Uroš M. and Mišić, Danijela and Despotović, Jovana and Samardžić, Jelena and Miljković, Đorđe and Timotijević, Gordana",
year = "2017",
abstract = "Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties. (C) 2017 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Functional Foods",
title = "Anti-encephalitogenic effects of cucumber leaf extract",
volume = "37",
pages = "249-262",
doi = "10.1016/j.jff.2017.07.060"
}

Jevtic, B., Djedovic, N., Stanisavljevic, S., Gašić, U. M., Mišić, D., Despotović, J., Samardžić, J., Miljković, Đ.,& Timotijević, G.. (2017). Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods
Elsevier Science Bv, Amsterdam., 37, 249-262.
https://doi.org/10.1016/j.jff.2017.07.060

Jevtic B, Djedovic N, Stanisavljevic S, Gašić UM, Mišić D, Despotović J, Samardžić J, Miljković Đ, Timotijević G. Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods. 2017;37:249-262.
doi:10.1016/j.jff.2017.07.060 .

Jevtic, Bojan, Djedovic, Neda, Stanisavljevic, Suzana, Gašić, Uroš M., Mišić, Danijela, Despotović, Jovana, Samardžić, Jelena, Miljković, Đorđe, Timotijević, Gordana, "Anti-encephalitogenic effects of cucumber leaf extract" in Journal of Functional Foods, 37 (2017):249-262,
https://doi.org/10.1016/j.jff.2017.07.060 . .

TY  - JOUR
AU  - Pavlović, Jelena
AU  - Samardžić, Jelena
AU  - Kostić, Ljiljana
AU  - Laursen, Kristian H.
AU  - Natić, Maja
AU  - Timotijević, Gordana
AU  - Schjoerring, Jan K.
AU  - Nikolić, Miroslav
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/2305
AB  - Background and Aims Retranslocation of iron (Fe) from source tissues enhances plant tolerance to Fe deficiency. Previous work has shown that silicon (Si) can alleviate Fe deficiency by enhancing acquisition and root to shoot translocation of Fe. Here the role of Si in Fe mobilization in older leaves and the subsequent retranslocation of Fe to young leaves of cucumber (Cucumis sativus) plants growing under Fe-limiting conditions was investigated. Methods Iron (Fe-57 or naturally occurring isotopes) was measured in leaves at different positions on plants hydroponically growing with or without Si supply. In parallel, the concentration of the Fe chelator nicotianamine (NA) along with the expression of nicotianamine synthase (NAS) involved in its biosynthesis and the expression of yellow stripe-like (YSL) transcripts mediating Fe-NA transport were also determined. Key Results In plants not receiving Si, approximately half of the total Fe content remained in the oldest leaf. In contrast, Si-treated plants showed an almost even Fe distribution among leaves with four different developmental stages, thus providing evidence of enhanced Fe remobilization from source leaves. This Si-stimulated Fe export was paralleled by an increased NA accumulation and expression of the YSL1 transporter for phloem loading/unloading of the Fe-NA complex. Conclusions The results suggest that Si enhances remobilization of Fe from older to younger leaves by a more efficient NA-mediated Fe transport via the phloem. In addition, from this and previous work, a model is proposed of how Si acts to improve Fe homeostasis under Fe deficiency in cucumber.
PB  - Oxford Univ Press, Oxford
T2  - Annals of Botany
T1  - Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions
VL  - 118
IS  - 2
SP  - 271
EP  - 280
DO  - 10.1093/aob/mcw105
ER  - 

@article{
author = "Pavlović, Jelena and Samardžić, Jelena and Kostić, Ljiljana and Laursen, Kristian H. and Natić, Maja and Timotijević, Gordana and Schjoerring, Jan K. and Nikolić, Miroslav",
year = "2016",
abstract = "Background and Aims Retranslocation of iron (Fe) from source tissues enhances plant tolerance to Fe deficiency. Previous work has shown that silicon (Si) can alleviate Fe deficiency by enhancing acquisition and root to shoot translocation of Fe. Here the role of Si in Fe mobilization in older leaves and the subsequent retranslocation of Fe to young leaves of cucumber (Cucumis sativus) plants growing under Fe-limiting conditions was investigated. Methods Iron (Fe-57 or naturally occurring isotopes) was measured in leaves at different positions on plants hydroponically growing with or without Si supply. In parallel, the concentration of the Fe chelator nicotianamine (NA) along with the expression of nicotianamine synthase (NAS) involved in its biosynthesis and the expression of yellow stripe-like (YSL) transcripts mediating Fe-NA transport were also determined. Key Results In plants not receiving Si, approximately half of the total Fe content remained in the oldest leaf. In contrast, Si-treated plants showed an almost even Fe distribution among leaves with four different developmental stages, thus providing evidence of enhanced Fe remobilization from source leaves. This Si-stimulated Fe export was paralleled by an increased NA accumulation and expression of the YSL1 transporter for phloem loading/unloading of the Fe-NA complex. Conclusions The results suggest that Si enhances remobilization of Fe from older to younger leaves by a more efficient NA-mediated Fe transport via the phloem. In addition, from this and previous work, a model is proposed of how Si acts to improve Fe homeostasis under Fe deficiency in cucumber.",
publisher = "Oxford Univ Press, Oxford",
journal = "Annals of Botany",
title = "Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions",
volume = "118",
number = "2",
pages = "271-280",
doi = "10.1093/aob/mcw105"
}

Pavlović, J., Samardžić, J., Kostić, L., Laursen, K. H., Natić, M., Timotijević, G., Schjoerring, J. K.,& Nikolić, M.. (2016). Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions. in Annals of Botany
Oxford Univ Press, Oxford., 118(2), 271-280.
https://doi.org/10.1093/aob/mcw105

Pavlović J, Samardžić J, Kostić L, Laursen KH, Natić M, Timotijević G, Schjoerring JK, Nikolić M. Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions. in Annals of Botany. 2016;118(2):271-280.
doi:10.1093/aob/mcw105 .

Pavlović, Jelena, Samardžić, Jelena, Kostić, Ljiljana, Laursen, Kristian H., Natić, Maja, Timotijević, Gordana, Schjoerring, Jan K., Nikolić, Miroslav, "Silicon enhances leaf remobilization of iron in cucumber under limited iron conditions" in Annals of Botany, 118, no. 2 (2016):271-280,
https://doi.org/10.1093/aob/mcw105 . .

TY  - DATA
AU  - Pavlović, Jelena
AU  - Samardžić, Jelena
AU  - Kostić, Ljiljana
AU  - Laursen, Kristian H.
AU  - Natić, Maja
AU  - Timotijević, Gordana
AU  - Schjoerring, Jan K.
AU  - Nikolić, Miroslav
PY  - 2016
UR  - https://cherry.chem.bg.ac.rs/handle/123456789/3608
PB  - Oxford Univ Press, Oxford
T2  - Annals of Botany
T1  - Supplementary data for the article: Pavlovic, J.; Samardzic, J.; Kostic, L.; Laursen, K. H.; Natic, M.; Timotijevic, G.; Schjoerring, J. K.; Nikolic, M. Silicon Enhances Leaf Remobilization of Iron in Cucumber under Limited Iron Conditions. Annals of Botany 2016, 118 (2), 271–280. https://doi.org/10.1093/aob/mcw105
UR  - https://hdl.handle.net/21.15107/rcub_cherry_3608
ER  - 

@misc{
author = "Pavlović, Jelena and Samardžić, Jelena and Kostić, Ljiljana and Laursen, Kristian H. and Natić, Maja and Timotijević, Gordana and Schjoerring, Jan K. and Nikolić, Miroslav",
year = "2016",
publisher = "Oxford Univ Press, Oxford",
journal = "Annals of Botany",
title = "Supplementary data for the article: Pavlovic, J.; Samardzic, J.; Kostic, L.; Laursen, K. H.; Natic, M.; Timotijevic, G.; Schjoerring, J. K.; Nikolic, M. Silicon Enhances Leaf Remobilization of Iron in Cucumber under Limited Iron Conditions. Annals of Botany 2016, 118 (2), 271–280. https://doi.org/10.1093/aob/mcw105",
url = "https://hdl.handle.net/21.15107/rcub_cherry_3608"
}

Pavlović, J., Samardžić, J., Kostić, L., Laursen, K. H., Natić, M., Timotijević, G., Schjoerring, J. K.,& Nikolić, M.. (2016). Supplementary data for the article: Pavlovic, J.; Samardzic, J.; Kostic, L.; Laursen, K. H.; Natic, M.; Timotijevic, G.; Schjoerring, J. K.; Nikolic, M. Silicon Enhances Leaf Remobilization of Iron in Cucumber under Limited Iron Conditions. Annals of Botany 2016, 118 (2), 271–280. https://doi.org/10.1093/aob/mcw105. in Annals of Botany
Oxford Univ Press, Oxford..
https://hdl.handle.net/21.15107/rcub_cherry_3608

Pavlović J, Samardžić J, Kostić L, Laursen KH, Natić M, Timotijević G, Schjoerring JK, Nikolić M. Supplementary data for the article: Pavlovic, J.; Samardzic, J.; Kostic, L.; Laursen, K. H.; Natic, M.; Timotijevic, G.; Schjoerring, J. K.; Nikolic, M. Silicon Enhances Leaf Remobilization of Iron in Cucumber under Limited Iron Conditions. Annals of Botany 2016, 118 (2), 271–280. https://doi.org/10.1093/aob/mcw105. in Annals of Botany. 2016;.
https://hdl.handle.net/21.15107/rcub_cherry_3608 .

Pavlović, Jelena, Samardžić, Jelena, Kostić, Ljiljana, Laursen, Kristian H., Natić, Maja, Timotijević, Gordana, Schjoerring, Jan K., Nikolić, Miroslav, "Supplementary data for the article: Pavlovic, J.; Samardzic, J.; Kostic, L.; Laursen, K. H.; Natic, M.; Timotijevic, G.; Schjoerring, J. K.; Nikolic, M. Silicon Enhances Leaf Remobilization of Iron in Cucumber under Limited Iron Conditions. Annals of Botany 2016, 118 (2), 271–280. https://doi.org/10.1093/aob/mcw105" in Annals of Botany (2016),
https://hdl.handle.net/21.15107/rcub_cherry_3608 .