TY  - DATA
AU  - Mutić, Tamara
AU  - Stanić-Vučinić, Dragana
AU  - Mutić, Jelena
AU  - Ilić, Miloš
AU  - Jovanović, Vesna
AU  - Aćimović, Jelena
AU  - Anđelković, Boban
AU  - Krishna de Guzman, Maria
AU  - Anđelković, Mirjana
AU  - Turkalj, Mirjana
AU  - Ćirković Veličković, Tanja
PY  - 2024
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6484
AB  - The experimental data for the results shown in the manuscript "Microplastic contamination of edible parts of commercially important mussels, clams and shrimps in different markets". Statistical investigation of the presence of microplastics in bivalve species using microFTIR. This dataset includes samples from various origins, including Sebia, South Korea, Croatia and Belgium, and includes species as diverse as shrimp, mussels and clams.
T1  - Research data no. 1 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets
UR  - https://hdl.handle.net/21.15107/rcub_cherry_6484
ER  - 

@misc{
author = "Mutić, Tamara and Stanić-Vučinić, Dragana and Mutić, Jelena and Ilić, Miloš and Jovanović, Vesna and Aćimović, Jelena and Anđelković, Boban and Krishna de Guzman, Maria and Anđelković, Mirjana and Turkalj, Mirjana and Ćirković Veličković, Tanja",
year = "2024",
abstract = "The experimental data for the results shown in the manuscript "Microplastic contamination of edible parts of commercially important mussels, clams and shrimps in different markets". Statistical investigation of the presence of microplastics in bivalve species using microFTIR. This dataset includes samples from various origins, including Sebia, South Korea, Croatia and Belgium, and includes species as diverse as shrimp, mussels and clams.",
title = "Research data no. 1 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets",
url = "https://hdl.handle.net/21.15107/rcub_cherry_6484"
}

Mutić, T., Stanić-Vučinić, D., Mutić, J., Ilić, M., Jovanović, V., Aćimović, J., Anđelković, B., Krishna de Guzman, M., Anđelković, M., Turkalj, M.,& Ćirković Veličković, T.. (2024). Research data no. 1 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets. .
https://hdl.handle.net/21.15107/rcub_cherry_6484

Mutić T, Stanić-Vučinić D, Mutić J, Ilić M, Jovanović V, Aćimović J, Anđelković B, Krishna de Guzman M, Anđelković M, Turkalj M, Ćirković Veličković T. Research data no. 1 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets. 2024;.
https://hdl.handle.net/21.15107/rcub_cherry_6484 .

Mutić, Tamara, Stanić-Vučinić, Dragana, Mutić, Jelena, Ilić, Miloš, Jovanović, Vesna, Aćimović, Jelena, Anđelković, Boban, Krishna de Guzman, Maria, Anđelković, Mirjana, Turkalj, Mirjana, Ćirković Veličković, Tanja, "Research data no. 1 for the manuscript: Microplastics contamination of edible parts of commercially relevant species of mussels, clams and shrimps across various markets" (2024),
https://hdl.handle.net/21.15107/rcub_cherry_6484 .

TY  - JOUR
AU  - Krishna de Guzman, Maria
AU  - Stanić-Vučinić, Dragana
AU  - Gligorijević, Nikola
AU  - Wimmer, Lukas
AU  - Gasparyan, Manvel
AU  - Lujić, Tamara
AU  - Vasović, Tamara
AU  - Dailey, Lea Ann
AU  - Van Haute, Sam
AU  - Ćirković-Veličković, Tanja
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6320
AB  - Human ingestion of microplastics (MPs) is common and inevitable due to the widespread contamination of food items, but implications on the gastric digestion of food proteins are still unknown. In this study, the interactions between pepsin and polystyrene (PS) MPs were evaluated by investigating enzyme activity and conformation in a simulated human gastric environment in the presence or absence of PS MPs. The impact on food digestion was also assessed by monitoring the kinetics of protein hydrolysis through static in vitro gastric digestion of cow's milk contaminated with PS. The binding of pepsin to PS showed that the surface chemistry of MPs dictates binding affinity. The key contributor to pepsin adsorption seems to be π−π interactions between the aromatic residues and the PS phenyl rings. During quick exposure (10 min) of pepsin to increasing concentrations (222, 2219, 22188 particles/mL) of 10 μm PS (PS10) and 100 μm PS (PS100), total enzymatic activities were not affected remarkably. However, upon prolonged exposure at 1 and 2 h, preferential binding of pepsin to the small, low zeta-potential PS caused structural changes in the protein which led to a significant reduction of its activity. Digestion of cow's milk mixed with PS10 resulted in transient accumulation of larger peptides (10–35 kDa) and reduced bioavailability of short peptides (2–9 kDa) in the gastric phase. This, however, was only observed at extremely high PS10 concentration (0.3 mg/mL or 5.46E+05 particles/mL). The digestion of milk peptides, bound preferentially over pepsin within the hard corona on the PS10 surface, was delayed up to 15 min in comparison to bulk protein digestion. Intact caseins, otherwise rapidly digested, remained bound to PS10 in the hard corona for up to 15 min. This work presents valuable insights regarding the interaction of MPs, food proteins, and pepsin, and their dynamics during gastric digestion.
PB  - Elsevier
T2  - Environmental Pollution
T1  - Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion
VL  - 335
SP  - 122282
DO  - 10.1016/j.envpol.2023.122282
ER  - 

@article{
author = "Krishna de Guzman, Maria and Stanić-Vučinić, Dragana and Gligorijević, Nikola and Wimmer, Lukas and Gasparyan, Manvel and Lujić, Tamara and Vasović, Tamara and Dailey, Lea Ann and Van Haute, Sam and Ćirković-Veličković, Tanja",
year = "2023",
abstract = "Human ingestion of microplastics (MPs) is common and inevitable due to the widespread contamination of food items, but implications on the gastric digestion of food proteins are still unknown. In this study, the interactions between pepsin and polystyrene (PS) MPs were evaluated by investigating enzyme activity and conformation in a simulated human gastric environment in the presence or absence of PS MPs. The impact on food digestion was also assessed by monitoring the kinetics of protein hydrolysis through static in vitro gastric digestion of cow's milk contaminated with PS. The binding of pepsin to PS showed that the surface chemistry of MPs dictates binding affinity. The key contributor to pepsin adsorption seems to be π−π interactions between the aromatic residues and the PS phenyl rings. During quick exposure (10 min) of pepsin to increasing concentrations (222, 2219, 22188 particles/mL) of 10 μm PS (PS10) and 100 μm PS (PS100), total enzymatic activities were not affected remarkably. However, upon prolonged exposure at 1 and 2 h, preferential binding of pepsin to the small, low zeta-potential PS caused structural changes in the protein which led to a significant reduction of its activity. Digestion of cow's milk mixed with PS10 resulted in transient accumulation of larger peptides (10–35 kDa) and reduced bioavailability of short peptides (2–9 kDa) in the gastric phase. This, however, was only observed at extremely high PS10 concentration (0.3 mg/mL or 5.46E+05 particles/mL). The digestion of milk peptides, bound preferentially over pepsin within the hard corona on the PS10 surface, was delayed up to 15 min in comparison to bulk protein digestion. Intact caseins, otherwise rapidly digested, remained bound to PS10 in the hard corona for up to 15 min. This work presents valuable insights regarding the interaction of MPs, food proteins, and pepsin, and their dynamics during gastric digestion.",
publisher = "Elsevier",
journal = "Environmental Pollution",
title = "Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion",
volume = "335",
pages = "122282",
doi = "10.1016/j.envpol.2023.122282"
}

Krishna de Guzman, M., Stanić-Vučinić, D., Gligorijević, N., Wimmer, L., Gasparyan, M., Lujić, T., Vasović, T., Dailey, L. A., Van Haute, S.,& Ćirković-Veličković, T.. (2023). Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion. in Environmental Pollution
Elsevier., 335, 122282.
https://doi.org/10.1016/j.envpol.2023.122282

Krishna de Guzman M, Stanić-Vučinić D, Gligorijević N, Wimmer L, Gasparyan M, Lujić T, Vasović T, Dailey LA, Van Haute S, Ćirković-Veličković T. Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion. in Environmental Pollution. 2023;335:122282.
doi:10.1016/j.envpol.2023.122282 .

Krishna de Guzman, Maria, Stanić-Vučinić, Dragana, Gligorijević, Nikola, Wimmer, Lukas, Gasparyan, Manvel, Lujić, Tamara, Vasović, Tamara, Dailey, Lea Ann, Van Haute, Sam, Ćirković-Veličković, Tanja, "Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion" in Environmental Pollution, 335 (2023):122282,
https://doi.org/10.1016/j.envpol.2023.122282 . .